CN210419821U

CN210419821U - Magnetic separation device for magnetic bead labeled HE4

Info

Publication number: CN210419821U
Application number: CN201920775894.XU
Authority: CN
Inventors: 逯岭松; 刘蓓; 冷建杭; 马霄
Original assignee: Hangzhou First Peoples Hospital
Current assignee: Hangzhou First Peoples Hospital
Priority date: 2019-05-27
Filing date: 2019-05-27
Publication date: 2020-04-28
Anticipated expiration: 2029-05-27

Abstract

The utility model provides a magnetic separation device that is used for magnetic bead marked HE4 belongs to the magnetic separation field. It has solved current magnetic separation device and has only had single function, and do not possess other functions, for example the scheduling problem of empting of supernatant, a magnetic separation device for magnetic bead mark's HE4, a serial communication port, including the support and set up the magnetic force subassembly on the support, magnetic force subassembly includes the base plate and can dismantle the magnetic force board of connection with the base plate, the base plate is provided with a plurality of chambeies that hold that are used for holding the centrifuging tube, the lateral wall that holds the chamber is provided with the mounting that is used for fixed centrifuging tube, be provided with on the magnetic force board and hold the magnet of chamber one-to-one, after magnetic force board and substrate connection, magnet is located the bottom that holds the chamber, the support includes the base and sets up in the branch of base both sides, be provided with the rotatory drive arrangement of drive branch on the base, the. The utility model has the advantages of multiple functions, etc.

Description

Magnetic separation device for magnetic bead labeled HE4

Technical Field

The utility model belongs to the magnetic separation field, in particular to a magnetic separation device that is used for magnetic bead marked HE 4.

Background

Ovarian cancer is a common malignant tumor of a female reproductive system, and Human epididymis-specific protein 4 (HE 4) is a tumor cell surface antigen of female ovarian cancer, has extremely low content in benign tumors and normal tissues, but has high content in ovarian cancer, and has been approved by FDA as a new tumor marker.

In the process of establishing the HE4 detection methodology in the laboratory, magnetic bead labeling of HE4 is involved, i.e., the biological magnetic beads and HE4 are incubated on a shaker at room temperature, HE4 which is not bound to the magnetic beads and HE4 which is bound to the magnetic beads need to be separated by a magnet, after separation, the supernatant is removed, PBS solution is added, and HE4 which is bound to the magnetic beads is suspended in PBS. The magnetic bead labeled HE4 suspended in PBS can be stored in a refrigerator for later use.

The existing magnetic separation device has only a single function, but does not have other functions, such as supernatant pouring and the like.

Disclosure of Invention

The utility model aims at providing a magnetic separation device that is used for magnetic bead labeled HE4 to the above-mentioned problem that exists among the prior art.

The purpose of the utility model can be realized by the following technical proposal: the utility model provides a magnetic separation device that is used for HE4 of magnetic bead mark, a serial communication port, include the support and set up the magnetic force subassembly on the support, the magnetic force subassembly include the base plate and can dismantle the magnetic plate of connection with the base plate, the base plate be provided with a plurality of chambeies that hold that are used for holding the centrifuging tube, the lateral wall that holds the chamber is provided with the mounting that is used for fixed centrifuging tube, be provided with on the magnetic plate and hold the magnet of chamber one-to-one, work as after magnetic plate and substrate connection, magnet is located the bottom that holds the chamber, the support include the base and set up in the branch of base both sides, the base on be provided with the rotatory drive arrangement of drive branch, the connection can be dismantled to the both sides of branch and base plate.

The utility model discloses a theory of operation: after the biological magnetic beads and HE4 are incubated on a shaking table at room temperature, the biological magnetic beads and HE4 are added into a centrifugal tube (generally an EP tube), then the centrifugal tube is placed into a containing cavity, a fixing part fixes the centrifugal tube to prevent the centrifugal tube from falling out of the containing cavity, magnets which correspond to the containing cavity one by one are arranged on a magnetic plate, the magnets are positioned at the bottom of the containing cavity after the magnetic plate is connected with a base plate, HE4 combined with the biological magnetic beads can be attached to the bottom of the centrifugal tube due to the attraction of the magnets, HE4 not combined with the biological magnetic beads is suspended in the centrifugal tube, after a period of time, a driving device drives a supporting rod to rotate, the supporting rod is connected with the base plate to drive the magnetic assembly to rotate, the magnetic assembly inclines, so that the centrifugal tube placed in the containing cavity inclines, supernatant can be poured and removed from the centrifugal tube, after the supernatant is removed, the magnetic assembly, the tube was then re-centrifuged and eluted with PBS solution, and the magnetic bead-bound HE4 was suspended in PBS. After the centrifuge tube is closed (typical centrifuge tubes are covered). The base plate and the centrifuge tube were stored together at a certain temperature. The base plate can be used as a centrifuge tube bracket at the moment, the centrifuge tube does not need to be taken down from the base plate, and the centrifuge tube and the base plate can be directly stored together. The utility model discloses a magnetic separation device has multiple functions, carries out magnetic separation promptly, can empty again and get rid of the supernatant, and wherein the base plate can also regard as a centrifuging tube support to support the centrifuging tube, has brought the convenience for the staff of experiment, has improved the efficiency of experiment.

In the magnetic separation device for HE4 labeled with magnetic beads, the insertion rods are arranged on both sides of the substrate, the fixing rods are arranged on the supporting rods, and the insertion rods can be inserted into the fixing rods to fix the substrate and the bracket.

In the magnetic separation device for the HE4 marked by the magnetic beads, the insertion rod is externally provided with an outer tube, the outer tube is provided with a sliding groove, the two ends of the sliding groove are provided with a round groove, the insertion rod is externally provided with a round bulge, the bulge is further provided with a sliding block, the sliding block can slide in the sliding groove, the bulge is used for being clamped with the round groove, and the bulge can be pressed by an operator.

In the above magnetic separation device for HE4 labeled with magnetic beads, the insertion rod is provided with an open slot, the open slot and the protrusion are connected by a spring, and the protrusion can be pressed into the open slot by an operator.

In the magnetic separation device for the HE4 marked by the magnetic beads, the insertion rod is further provided with a clamping block, the fixing rod is provided with a groove for inserting the insertion rod, one side of the groove is provided with a clamping groove, and the clamping block is used for clamping with the clamping groove and preventing the insertion rod from rotating.

In the magnetic separation device for HE4 labeled with magnetic beads, the bottom of the substrate is provided with an insertion slot for inserting the magnetic plate, the two sides of the substrate are provided with rotating rods for fixing the magnetic plate, the substrate is provided with a shaft lever, the rotating rods can rotate around the shaft lever, a friction layer is arranged between the rotating rods and the shaft lever, and the friction layer is used for preventing the rotating rods from freely rotating.

In the magnetic separation device for the magnetic bead labeled HE4, the fixing member is a rubber ring, the rubber ring is fixedly connected with the peripheral wall of the accommodating cavity, and the rubber ring is pressed against the peripheral wall of the centrifugal tube and fixes the centrifugal tube.

In the magnetic separation device for the magnetic bead labeled HE4, the magnet is embedded in the magnetic plate, and the top surface of the magnet is flush with the top surface of the magnetic plate.

In the magnetic separation device for magnetic bead labeled HE4, the accommodating chambers are arranged in parallel.

Compared with the prior art, the utility model has the advantages of it is following:

1. utility model's magnetic separation device has multiple functions, carries out magnetic separation promptly, can empty again and get rid of the supernatant, and wherein the base plate can also regard as a centrifuging tube support to support the centrifuging tube, has brought the convenience for the staff of experiment, has improved the efficiency of experiment.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

fig. 2 is a schematic structural diagram of the substrate of the present invention;

FIG. 3 is a schematic structural view of the magnetic plate of the present invention;

FIG. 4 is a schematic view of the structure of the insertion rod and the outer tube of the present invention;

fig. 5 is a schematic structural diagram of the open slot of the present invention;

fig. 6 is a schematic structural diagram of the friction layer of the present invention.

In the figure, 1, a bracket; 2. a magnetic assembly; 3. a substrate; 4. a magnetic plate; 5. an accommodating chamber; 6. a fixing member; 7. a magnet; 8. a base; 9. a strut; 10. a drive device; 11. an insertion rod; 12. fixing the rod; 13. an outer tube; 14. a chute; 15. a round slot; 16. a protrusion; 17. a slider; 18. an open slot; 19. a spring; 20. clamping the block; 21. a groove; 22. a clamping groove is formed; 23. rotating the rod; 24. a shaft lever; 25. a friction layer; 26. a rotating shaft; 27. and inserting the groove.

Detailed Description

The following are specific embodiments of the present invention and the accompanying drawings are used to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

As shown in fig. 1-6, a magnetic separation device for HE4 marked by magnetic beads, including support 1 and the magnetic force component 2 arranged on support 1, magnetic force component 2 includes base plate 3 and magnetic plate 4 detachably connected with base plate 3, base plate 3 is provided with a plurality of holding cavities 5 for holding centrifuge tubes, the side wall holding cavity 5 is provided with fixing parts 6 for fixing centrifuge tubes, magnet 7 one-to-one corresponding to holding cavity 5 is arranged on magnetic plate 4, after magnetic plate 4 and base plate 3 are connected, magnet 7 is located the bottom of holding cavity 5, support 1 includes base 8 and support rod 9 arranged on both sides of base 8, drive device 10 for driving support rod 9 to rotate is arranged on base 8, and support rod 9 and base plate 3 are detachably connected on both sides.

The drive means 10 may be a motor. The motor is provided with a rotating shaft 26, and the rotating shaft 26 is connected with the bottom of the supporting rod 9. The base 8 is covered outside the motor 10

More specifically, the insertion rods 11 are disposed on both sides of the substrate 3, the fixing rods 12 are disposed on the supporting rods 9, and the insertion rods 11 can be inserted into the fixing rods 12 to fix the substrate 3 and the bracket 1. The outer tube 13 is further sleeved outside the insertion rod 11, the sliding groove 14 is formed in the outer tube 13, round grooves 15 are formed in two ends of the sliding groove 14, a round protrusion 16 is arranged outside the insertion rod 11, a sliding block 17 is further arranged on the protrusion 16, the sliding block 17 can slide in the sliding groove 14, the protrusion 16 is used for being clamped with the round grooves 15, and the protrusion 16 can be pressed by an operator. The insertion rod 11 is provided with an open slot 18, the open slot 18 and the protrusion 16 are connected by a spring 19, and the protrusion 16 can be pressed into the open slot 18 by an operator. The inserting rod 11 is further provided with a clamping block 20, the fixing rod 12 is provided with a groove 21 for the inserting rod 11 to insert, one side of the groove 21 is provided with a clamping groove 22, and the clamping block 20 is used for being clamped with the clamping groove 22 and preventing the inserting rod 11 from rotating.

During installation, the insertion rod 11 is aligned with the groove 21, the clamping block 20 is aligned with the clamping groove 22, an operator presses the protrusion 16, the protrusion 16 is pressed into the open groove 18, the protrusion 16 is separated from the circular groove 15 on the outer tube 13, the sliding block 17 is pushed to enable the sliding block 17 to slide along the sliding groove 14, the protrusion 16 is pushed into the circular groove 15 on the other side, after hands are placed on the device, the protrusion 16 is clamped into the circular groove 15 on the other side due to the elastic force of the spring 19, the insertion rod 11 is inserted into the groove 21 at the moment, the clamping block 20 is clamped with the clamping groove 22, and the insertion rod 11 on the other side of the substrate 3 is also placed into the insertion fixing rod 12 according to the method. So that the base plate 3 and the support 9 are fixed and the snap-in blocks 20 prevent the base plate 3 from rotating relative to the support 9, the base plate 3 rotating with the support 9. Similarly, the operator presses the protrusion 16 to move the protrusion 16 out of the circular slot 15 at the front end of the outer tube 13 and out of the circular slot 15 at the rear end, i.e., the insertion rod 11 moves out of the groove 21.

In more detail, the bottom of the base plate 3 is provided with an insertion groove 27 for inserting the magnetic plate 4, the two sides of the base plate 3 are provided with a rotating rod 23 for fixing the magnetic plate 4, the base plate 3 is provided with a shaft rod 24, the rotating rod 23 can rotate around the shaft rod 24, a friction layer 25 is arranged between the rotating rod 23 and the shaft rod 24, and the friction layer 25 is used for preventing the rotating rod 23 from freely rotating. The operator manually breaks the rotating lever 23 to rotate the rotating lever 23 against the friction force, but the gravity of the rotating lever 23 cannot overcome the friction force, so that the rotating lever 23 cannot rotate freely. After the magnetic plate 4 is inserted into the insertion slot 27, the operator pulls the rotating lever 23 so that the rotating lever 23 rotates against the surface of the magnetic plate 4, preventing the magnetic plate 4 from being removed from the insertion slot 27.

Further saying in a detailed way, mounting 6 is the rubber circle, rubber circle and the perisporium fixed connection who holds chamber 5, and the rubber circle supports presses on the periphery wall of centrifuging tube and fixed centrifuging tube. After the centrifugal tube is inserted into the accommodating cavity 5, because the rubber ring is fixedly connected with the peripheral wall of the accommodating cavity 5, after the centrifugal tube is inserted, the peripheral wall of the centrifugal tube extrudes the rubber ring, so that the rubber ring is deformed, and friction force exists between the rubber ring and the centrifugal tube,

in further detail, the magnet 7 is embedded on the magnetic plate 4, and the top surface of the magnet 7 is flush with the top surface of the magnetic plate 4. Facilitating insertion of magnetic plate 4 from insertion slot 27.

The accommodation chambers 5 are arranged in parallel. The receiving chambers 5 are arranged in parallel, so that the centrifuge tubes are also arranged in parallel. Is relatively tidy.

The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications, additions and substitutions for the specific embodiments described herein may be made by those skilled in the art without departing from the spirit of the invention or exceeding the scope of the invention as defined in the accompanying claims.

Although the terms of the bracket 1, the magnetic assembly 2, the base plate 3, the magnetic plate 4, the accommodating cavity 5, the fixing member 6, the magnet 7, the base 8, the supporting rod 9, the driving device 10, the insertion rod 11, the fixing rod 12, the outer tube 13, the sliding groove 14, the circular groove 15, the protrusion 16, the sliding block 17, the open groove 18, the spring 19, the snap-in block 20, the groove 21, the snap-in groove 22, the rotating rod 23, the shaft rod 24, the friction layer 25, the rotating shaft 26, the insertion groove 27, etc. are used more frequently, the possibility of using other terms is not excluded. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed in a manner that is inconsistent with the spirit of the invention.

Claims

1. The utility model provides a magnetic separation device that is used for HE4 of magnetic bead mark, its characterized in that includes support (1) and sets up magnetic force component (2) on support (1), magnetic force component (2) include base plate (3) and can dismantle magnetic force board (4) of being connected with base plate (3), base plate (3) be provided with a plurality of holding chamber (5) that are used for holding the centrifuging tube, the lateral wall that holds chamber (5) is provided with mounting (6) that are used for fixed centrifuging tube, be provided with on magnetic force board (4) and hold chamber (5) one-to-one magnet (7), work as magnetic force board (4) and base plate (3) are connected the back, magnet (7) are located the bottom that holds chamber (5), support (1) include base (8) and set up branch (9) in base (8) both sides, base (8) on be provided with rotatory drive arrangement (10) of drive branch (9), the support rod (9) is detachably connected with the two sides of the base plate (3).

2. The magnetic separation device for the HE4 labeled with magnetic beads as claimed in claim 1, wherein the substrate (3) is provided with insertion rods (11) on both sides, the support rod (9) is provided with a fixing rod (12), and the insertion rods (11) can be inserted into the fixing rod (12) to fix the substrate (3) and the support (1).

3. The magnetic separation device for the HE4 labeled by the magnetic beads as claimed in claim 2, wherein an outer tube (13) is further sleeved outside the insertion rod (11), a sliding groove (14) is formed in the outer tube (13), circular groove slots (15) are formed in two ends of the sliding groove (14), a circular protrusion (16) is arranged outside the insertion rod (11), a sliding block (17) is further arranged on the protrusion (16), the sliding block (17) can slide in the sliding groove (14), the protrusion (16) is used for being clamped with the circular groove slots (15), and the protrusion (16) can be pressed by an operator.

4. The magnetic separation device for magnetic bead labeled HE4 as claimed in claim 2, wherein the insertion rod (11) is provided with an open slot (18), the open slot (18) and the protrusion (16) are connected by a spring (19), and the protrusion (16) can be pressed into the open slot (18) by an operator.

5. The magnetic separation device for the HE4 labeled with magnetic beads as claimed in claim 2, wherein the insertion rod (11) is further provided with a latch block (20), the fixing rod (12) is provided with a groove (21) for the insertion rod (11) to insert into, one side of the groove (21) is provided with a latch slot (22), and the latch block (20) is used for latching with the latch slot (22) and preventing the insertion rod (11) from rotating.

6. The magnetic separation device for the magnetic bead labeled HE4 according to claim 1, wherein the bottom of the base plate (3) is provided with an insertion slot (27) for inserting the magnetic plate (4), the two sides of the base plate (3) are provided with rotating rods (23) for fixing the magnetic plate (4), the base plate (3) is provided with a shaft rod (24), the rotating rods (23) can rotate around the shaft rod (24), a friction layer (25) is arranged between the rotating rods (23) and the shaft rod (24), and the friction layer (25) is used for preventing the rotating rods (23) from freely rotating.

7. The magnetic separation device for the HE4 labeled with magnetic beads as claimed in claim 1, wherein the fixing member (6) is a rubber ring fixedly connected to the peripheral wall of the containing chamber (5), and the rubber ring presses against the peripheral wall of the centrifuge tube and fixes the centrifuge tube.

8. The magnetic separation device for the HE4 labeled with magnetic beads of claim 1, wherein the magnet (7) is embedded on the magnetic plate (4), and the top surface of the magnet (7) is flush with the top surface of the magnetic plate (4).

9. The magnetic separation device for the magnetic bead labeled HE4 of claim 1, wherein the receiving cavities are arranged in parallel.