CN210401156U

CN210401156U - Flow cytometer

Info

Publication number: CN210401156U
Application number: CN201921170154.XU
Authority: CN
Inventors: 闵少雄; 赵胜利; 邱素均; 史本超; 殷杰; 张国炜; 于庆贺
Original assignee: Southern Medical University Zhujiang Hospital
Current assignee: Southern Medical University Zhujiang Hospital
Priority date: 2019-07-24
Filing date: 2019-07-24
Publication date: 2020-04-24
Anticipated expiration: 2029-07-24

Abstract

The utility model discloses a flow cytometer, which comprises a fixed cover, a mounting ring and a limit cover, wherein the middle parts of the two sides of the fixed cover, which are close to the top end, are fixedly connected with a mounting plate through bolts; the utility model discloses an at the inside stand pipe that is equipped with of fixed cover, and implement the location through ring gear and ball, make motor drive back-up bar drive gear drive ring gear and drive the stand pipe when implementing the rotation in fixed cover, can effectually make the cell suspension when implementing the transmission through spacing cover and transmission pipe, can implement effectual drive to the sheath liquid that carries through the conveyer pipe and make, the drainage can be implemented through the drive plate to the sheath liquid when the transmission in the stand pipe, thereby can effectually make the sheath liquid implement the effect of effectual parcel drainage to the cell suspension, thereby make the cell suspension implement the detection effect of the more accurate effectual improvement cell suspension of position precision when spraying the detection and then compensate not enough among the prior art.

Description

Flow cytometer

Technical Field

The utility model relates to a cell appearance technical field specifically is a flow cytometer.

Background

Flow cytometers are devices that automatically analyze and sort cells. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to the preselected parameter range. Most flow cytometers are zero-resolution instruments, which can only measure indexes such as total nucleic acid amount and total protein amount of a cell, but cannot identify and measure the amount of nucleic acid or protein in a specific part, and a flow chamber is an important component of the flow cytometers, through which cells can be effectively promoted to be effectively transported, so that the detection effect of the cells can be conveniently implemented, while in the prior art, certain defects exist when the cells are transported through sheath fluid in the flow cytometers, such as:

when the flow cytometer in the prior art transfers and sprays cell suspension through sheath liquid through the flow chamber, the cell suspension is easy to deviate due to incomplete sheath liquid wrapping during transportation, so that detection deviation of the cell suspension is easy to occur during detection, and the requirements of the prior art cannot be met.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a flow cytometer to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a flow cytometer comprises a fixed cover, a mounting ring and a limiting cover, wherein the middle parts of the two sides of the fixed cover are fixedly connected with a mounting plate by bolts near the top end, the middle part of the lower surface of the mounting plate is fixedly connected with a motor by bolts, the power output end of the motor is fixedly connected with a support rod by a coupler, the bottom end of the support rod is fixedly connected with a gear by bolts, the inner wall of the fixed cover is inserted and connected with a gear ring near the bottom end, one end of the gear penetrates through the fixed cover to be meshed with the gear ring, the top end of the inner wall of the fixed cover is fixedly connected with the mounting ring by bolts, a guide pipe is inserted and connected in the fixed cover, the peripheral bottom end of the guide pipe is fixedly connected with the gear ring by bolts, the edge of the top end of the guide pipe is embedded with balls, the guide pipe, the middle of the lower surface of the limiting cover is fixedly connected with a transmission pipe through a bolt, the top end of the transmission pipe is communicated with the limiting cover, the lower surfaces of the limiting covers on two sides of the transmission pipe are fixedly connected with transmission pipes through bolts, and the lower surfaces of the transmission pipes are fixedly connected with the fixing cover through bolts.

Preferably, the middle parts of the two sides of the fixed cover, which are close to the bottom end, are fixedly connected with limit frames through bolts, the middle parts of the limit frames are rotatably connected with the supporting rods through bearings, and the limit frames are used as limit devices of the supporting rods.

Preferably, the fixed cover is hollow cylinder type, the mounting panel is the quadrangular prism type, the motor is connected with external power supply electric property, branch length dimension is not more than fixed cover length dimension.

Preferably, the gear ring with fixed cover is clearance fit, two the gear all with fixed cover is clearance fit, the gear is used for the drive arrangement as gear ring, gear ring internal diameter size with fixed cover internal diameter size is the same.

Preferably, the drive plate is in a quadrangular frustum pyramid shape, the length of the drive plate is not greater than that of the guide pipe, the length of the guide pipe is not greater than that of the fixed cover, the inner wall of the guide pipe is in a frustum cone shape, and the gear ring is used as a transmission device for driving the guide pipe by a gear.

Preferably, a cover plate is fixedly connected between the conveying pipe and the fixing cover through bolts, the cover plate is used as a sealing device between the fixing cover and the conveying pipe, the limiting cover is disc-shaped, and the middle part of the upper surface of the limiting cover is conical.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model discloses an at the inside stand pipe that is equipped with of fixed cover, and implement the location through ring gear and ball, make motor drive back-up bar drive gear drive ring gear and drive the stand pipe when implementing the rotation in fixed cover, can effectually make the cell suspension when implementing the transmission through spacing cover and transmission pipe, can implement effectual drive to the sheath liquid that carries through the conveyer pipe and make, the drainage can be implemented through the drive plate to the sheath liquid when the transmission in the stand pipe, thereby can effectually make the sheath liquid implement the effect of effectual parcel drainage to the cell suspension, thereby make the cell suspension implement the detection effect of the more accurate effectual improvement cell suspension of position precision when spraying the detection and then compensate not enough among the prior art.

Drawings

FIG. 1 is a schematic view of the whole half-section structure of the present invention;

FIG. 2 is a schematic view of the structure of the present invention;

FIG. 3 is a schematic top view of the present invention;

fig. 4 is a schematic view of the bottom view of the present invention.

In the figure: 1-fixing the cover; 11-a mounting plate; 12-a motor; 13-a strut; 14-a gear; 15-a gear ring; 16-a limiting frame; 2-mounting a ring; 21-a ball; 22-a guide tube; 23-a drive plate; 3-a limit cover; 31-a transfer pipe; 32-conveying pipe.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: a flow cytometer comprises a fixed cover 1, a mounting ring 2 and a limiting cover 3, wherein the middle parts of the two sides of the fixed cover 1 are close to the top end and are respectively fixedly connected with a mounting plate 11 through bolts, the mounting plate 11 is of a quadrangular prism shape, the fixed cover 1 is of a hollow cylindrical shape, the middle part of the lower surface of the mounting plate 11 is fixedly connected with a motor 12 through bolts, the motor 12 is electrically connected with an external power supply, the power output end of the motor 12 is fixedly connected with a support rod 13 through a coupler, the support rod 13 can be effectively controlled and driven to rotate through the motor 12, the length of the support rod 13 is not more than that of the fixed cover 1, the middle parts of the two sides of the fixed cover 1 are close to the bottom end and are respectively fixedly connected with a limiting frame 16 through bolts, the middle part of the limiting frame 16 is rotatably connected with the support rod, the positioning of the support rod 13 can be effectively realized through the limiting frame 16, so that the support rod 13 is more stable when rotating, the bottom end of the support rod 13 is fixedly connected with a gear 14 through a bolt, a gear ring 15 is inserted at the position, close to the bottom end, of the inner wall of the fixed cover 1, the inner diameter of the gear ring 15 is the same as that of the fixed cover 1, the gear ring 15 is in clearance fit with the fixed cover 1, so that the gear ring 15 can effectively rotate in the fixed cover 1, one end of the gear 14 penetrates through the fixed cover 1 to be meshed with the gear ring 15, the two gears 14 are in clearance fit with the fixed cover 1, the gear 14 is used as a driving device of the gear ring 15, the top end of the inner wall of the fixed cover 1 is fixedly connected with an installation ring 2 through a bolt, a guide pipe 22 is inserted in the fixed cover 1, and the guide pipe 22 rotates in the, the length of the drive plate 23 is not greater than the length of the guide tube 22, the length of the guide tube 22 is not greater than the length of the fixed cover 1, the inner wall of the guide tube 22 is in a circular truncated cone shape, the peripheral bottom end of the guide tube 22 is fixedly connected with the gear ring 15 through a bolt, the gear ring 15 is used as a transmission device for driving the guide tube 22 through the gear 14, the edge of the top end of the guide tube 22 is inlaid with a ball 21, the guide tube 22 is rotatably connected with the lower surface of the mounting ring 2 through the ball 21, the positioning effect of the guide tube 22 inside the fixed cover 1 can be effectively achieved through the ball 21, the middle part of the inner wall of the guide tube 22 is fixedly connected with the drive plate 23 through a bolt, the drive plate 23 is in a four-truncated-pyramid shape, the guiding effect of sheath liquid conveyed in the conveying tube 32 can be effectively achieved through the drive plate 23, the middle part of, the bottom end of the transmission pipe 31 penetrates through the mounting ring 2 and is inserted into the guide pipe 22, the limiting cover 3 is disc-shaped, the middle of the upper surface of the limiting cover 3 is conical, the top end of the transmission pipe 31 is communicated with the limiting cover 3, the lower surfaces of the limiting covers 3 on two sides of the transmission pipe 31 are fixedly connected with a transmission pipe 32 through bolts, the lower surface of the transmission pipe 32 is fixedly connected with the fixed cover 1 through bolts, a cover plate is fixedly connected between the transmission pipe 32 and the fixed cover 1 through bolts, and the cover plate is used as a sealing device between the fixed cover 1 and the transmission pipe 32.

The working principle is as follows: when in use, the limiting cover 3 is fixedly arranged in the cell suspension injection device of the flow cytometer, so that the cell suspension can be effectively concentrated inside the limiting cover 3 and transmitted to the guide tube 22 through the transmission tube 31, one end of the transmission tube 32 is fixedly connected with the sheath liquid transmission port, so that the sheath liquid can be transmitted to the guide tube 22 through the transmission tube 31, the strut 13 is driven by the motor 12, the strut 13 drives the gear 14 and drives the gear ring 15, so that the guide tube 22 can implement directional rotation inside the fixed cover 1 through the balls 21, and the driving plate 23 inside the guide tube 22 can implement effective driving on the sheath liquid transmitted inside the transmission tube 32, so that the sheath liquid can be effectively transmitted to the cell suspension.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a flow cytometer, includes fixed cover (1), collar (2) and spacing cover (3), its characterized in that: the middle parts of two sides of the fixed cover (1) are fixedly connected with a mounting plate (11) through bolts, the middle part of the lower surface of the mounting plate (11) is fixedly connected with a motor (12) through bolts, the power output end of the motor (12) is fixedly connected with a supporting rod (13) through a coupler, the bottom end of the supporting rod (13) is fixedly connected with a gear (14) through bolts, a gear ring (15) is inserted in the position of the inner wall of the fixed cover (1) close to the bottom end, one end of the gear (14) penetrates through the fixed cover (1) to be meshed with the gear ring (15), the top end of the inner wall of the fixed cover (1) is fixedly connected with a mounting ring (2) through bolts, a guide pipe (22) is inserted in the fixed cover (1), the peripheral bottom end of the guide pipe (22) is fixedly connected with the gear ring (15) through bolts, the guide pipe (22) is rotatably connected with the lower surface of the mounting ring (2) through balls (21), the middle part of the inner wall of the guide pipe (22) is fixedly connected with a drive plate (23) through bolts, the middle part of the lower surface of the limiting cover (3) is fixedly connected with a transmission pipe (31) through bolts, the top end of the transmission pipe (31) is mutually communicated with the limiting cover (3), the lower surfaces of the limiting covers (3) on the two sides of the transmission pipe (31) are fixedly connected with conveying pipes (32) through bolts, and the lower surfaces of the conveying pipes (32) are fixedly connected with the fixing cover (1) through bolts.

2. A flow cytometer as described in claim 1 wherein: the middle parts of the two sides of the fixed cover (1) are close to the bottom end and are fixedly connected with a limiting frame (16) through bolts, the middle parts of the limiting frame (16) are rotatably connected with the supporting rods (13) through bearings, and the limiting frame (16) is used as a limiting device of the supporting rods (13).

3. A flow cytometer as described in claim 1 wherein: fixed cover (1) is hollow cylinder type, mounting panel (11) are the quadrangular prism type, motor (12) and external power source electric connection, branch (13) length dimension is not more than fixed cover (1) length dimension.

4. A flow cytometer as described in claim 1 wherein: gear ring (15) with fixed cover (1) is clearance fit, two gear (14) all with fixed cover (1) is clearance fit, gear (14) are used for as the drive arrangement of gear ring (15), gear ring (15) internal diameter size with fixed cover (1) internal diameter size is the same.

5. A flow cytometer as described in claim 1 wherein: the driving plate (23) is in a quadrangular frustum pyramid shape, the length dimension of the driving plate (23) is not larger than that of the guide pipe (22), the length dimension of the guide pipe (22) is not larger than that of the fixed cover (1), the inner wall of the guide pipe (22) is in a circular truncated cone shape, and the gear ring (15) is used as a transmission device for driving the guide pipe (22) through the gear (14).

6. A flow cytometer as described in claim 1 wherein: conveyer pipe (32) with through bolt fixedly connected with apron between fixed cover (1), the apron be used for as fixed cover (1) with sealing device between conveyer pipe (32), spacing cover (3) are the disc, spacing cover (3) upper surface middle part is the circular cone type.