CN210394317U

CN210394317U - DNA synthesis plate

Info

Publication number: CN210394317U
Application number: CN201920875845.3U
Authority: CN
Inventors: 秦伟; 姜燕平; 陈高明; 刘祺霞; 胡玉梅
Original assignee: Biocomma Biotech Co ltd
Current assignee: Biocomma Biotech Co ltd
Priority date: 2019-06-11
Filing date: 2019-06-11
Publication date: 2020-04-24
Anticipated expiration: 2029-06-11

Abstract

The utility model discloses a DNA synthesis board, which comprises a plurality of assembly pipes, wherein each assembly pipe is internally provided with a first assembly position, a second assembly position and a third assembly position in sequence, and the inner diameter of each assembly pipe is reduced in a gradient manner from the first assembly position to the third assembly position; a CPG carrier assembled within the first assembly site or the second assembly site or the third assembly site of each of the assembly tubes. The technical proposal of the utility model aims at improving the compatibility of the DNA synthesis plate.

Description

DNA synthesis plate

Technical Field

The utility model relates to a DNA synthesis technical field, in particular to a DNA synthesis board.

Background

With the rapid development of gene drug screening, functional genomics, proteomics, synthetic biology, and clinical diagnostics, massive DNA synthesis is required, and rapid synthesis of genes has become the heat of synthetic biology. Compared with single-column tubes, high-flux 96-well plates, 384-well plates and 1536-well plates are a development trend of rapid synthesis.

The current DNA synthesis plate is mainly designed for conventional DNA synthesis, for example, more than or equal to 25nmol, is mainly used for conventional DNA synthesis, and is not suitable for DNA synthesis of 0.1-10 nmol. Currently, the mainstream DNA synthesizers include dr. oligo192 series from Biolytic, MM192 series from Bioautomation, TAG Copenhagen a/S Oligomaker 192 series, and 192 series from Thermo Fisher, and several commercially available DNA synthesizers are not fully compatible or universal. Meanwhile, one synthetic plate can satisfy only conventional or minute amounts of DNA synthesis, cannot satisfy both conventional and/or minute amounts of DNA synthesis, and causes waste of resources due to liquid residues, due to problems in design.

The above is only for the purpose of assisting understanding of the technical solutions of the present application, and does not represent an admission that the above is prior art.

SUMMERY OF THE UTILITY MODEL

The main object of the present invention is to provide a DNA synthesis plate, which aims to improve the compatibility of the DNA synthesis plate.

To achieve the above object, the present invention provides a DNA synthesis plate comprising:

a plurality of fitting pipes, each of which has a first fitting position, a second fitting position, and a third fitting position formed therein in this order, and whose inner diameter decreases in a gradient from the first fitting position to the third fitting position;

a CPG carrier assembled within the first assembly site or the second assembly site or the third assembly site of each of the assembly tubes.

Further, a first transition pipe is arranged between the first assembling position and the second assembling position, the first assembling position and the second assembling position are communicated through the first transition pipe, the inner diameter of the first transition pipe is gradually reduced from the first assembling position to the second assembling position, the CPG carrier comprises a first carrier and a second carrier, the first carrier is located at the first assembling position, and the second carrier is located at the second assembling position.

Further, an included angle formed by the inner wall of the first assembling position and the inner wall of the first transition pipe is defined as α 1 degrees, and the included angle is more than 90 degrees and less than α 1 degrees and less than 180 degrees;

or the joint of the inner wall of the first assembling position and the inner wall of the first transition pipe is in arc transition arrangement.

Furthermore, a second transition pipe is arranged between the second assembling position and the third assembling position, the second assembling position and the third assembling position are communicated through the second transition pipe, the inner diameter of the second transition pipe is gradually reduced from the second assembling position to the third assembling position, the CPG carrier further comprises a third carrier, and the third carrier is located at the third assembling position.

Further, an included angle formed by the second assembling position and the inner wall of the second transition pipe is defined as α 2 degrees, and 90 degrees is more than α 2 degrees and less than 180 degrees;

or the joint of the second assembling position and the inner wall of the second transition pipe is in arc transition arrangement.

Further, the second fitting position has an inner diameter of 0.1mm to 9.0mm, the second fitting position has a height of 0.5mm to 11.0mm in the axial direction of the fitting pipe, the third fitting position has an inner diameter of 0.1mm to 5.0mm, and the third fitting position has a height of 0.5mm to 8.0mm in the axial direction of the fitting pipe.

Furthermore, the diameter of the second carrier is less than or equal to 8.8mm, the thickness of the second carrier is 0.5mm-11.0 mm, the diameter of the third carrier is less than or equal to 4.8mm, and the thickness of the third carrier is 0.5mm-8.0 mm.

Further, one end of the assembling pipe adjacent to the third assembling position is a liquid outlet, and the liquid outlet is of an inclined opening structure, a flat opening structure or a sharp opening structure.

Furthermore, the DNA synthesis plate also comprises a frame for connecting the assembly pipes, and the frame and the limiting pipes are of an integrally formed structure.

Further, the frame is a cuboid, the length of the frame is 60 mm-140 mm, the width of the frame is 40 mm-110 mm, and the height of the frame is 8 mm-50 mm.

The utility model discloses among the technical scheme, first assembly position, second assembly position and third assembly position are formed in proper order in the spacing intraductal, and three assembly position is used for assembling the CPG carrier of different specifications respectively, and a DNA synthetic board realizes simultaneously a large amount of and/or conventional and/or trace DNA's synthesis to improve the compatibility of DNA synthetic board. The inner diameter of the assembly pipe is in a structural design of reducing in a gradient manner, so that liquid can flow down along the inner wall, the residue of the liquid is reduced, the using amount of a synthetic reagent can be effectively saved, the cost is reduced, and the gradual amplification effect caused by the residue of the liquid can be avoided; and the assembly pipe is cylindricly set up, makes things convenient for the machine to realize the assembly of CPG carrier, is favorable to realizing the automation, and then improves assembly efficiency.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic structural view of an embodiment of the DNA synthesis plate of the present invention;

FIG. 2 is a cross-sectional view of the DNA synthesis plate of FIG. 1;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

FIG. 4 is a schematic structural view of an embodiment of a partial structure of the DNA synthesis plate of FIG. 1;

FIG. 5 is a schematic structural view of another example of a partial structure of the DNA synthesis plate of FIG. 1.

The reference numbers illustrate:

reference numerals	Name (R)	Reference numerals	Name (R)
				100	DNA synthesis plate	14	First transition pipe
10	Assembling pipe	15	Second transition pipe
				11	First assembly station	20	Rims
12	Second assembly position	40	Third carrier
				13	Second assembly position

The objects, features and advantages of the present invention will be further described with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that all the directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.

In the present application, unless expressly stated or limited otherwise, the terms "connected" and "fixed" are to be construed broadly, e.g., "fixed" may be fixedly connected or detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

The utility model provides a DNA synthesis board 100.

Referring to fig. 1 to 3, the DNA synthesis plate 100 includes a plurality of assembly tubes 10 and CPG carriers, the assembly tubes 10 are sequentially formed with a first assembly line 11, a second assembly line 12 and a third assembly line 13 in each assembly tube 10, and the inner diameter of the assembly tubes decreases in a gradient from the first assembly line 11 to the third assembly line 13, and the CPG carriers are assembled in the first assembly line 11, the second assembly line 11 or the third assembly line 13 of each assembly tube 10.

In this embodiment, the DNA synthesis plate 100 can be used for oligonucleotide (oligo) synthesis. Specifically, the DNA synthesis plate 100 is provided with 96 assembling tubes 10, and the 96 assembling tubes 10 are arranged at even intervals so that the structural strength of each portion is equal, thereby making the entire structure uniform. The first assembly site 11 is designed for mass DNA synthesis on the μmol scale; the second assembly site 12 is designed for conventional DNA synthesis at a scale of 25-200 nmol; the third assembly site 13 is designed for the synthesis of a trace amount of DNA, and the synthesis scale is 0 to 10 nmol.

The utility model discloses among the technical scheme, form first assembly position 11, second assembly position 12 and third assembly position 13 in proper order in the spacing pipe 10, three assembly position is used for assembling the CPG carrier of different specifications respectively, and a DNA synthetic board 100 realizes the synthesis of a large amount of and/or conventional and/or trace DNA simultaneously to improve the compatibility of DNA synthetic board 100. The inner diameter of the assembly pipe 10 is in a structural design of reducing the gradient, so that liquid can flow down along the inner wall, the residue of the liquid is reduced, the using amount of a synthetic reagent can be effectively saved, the cost is reduced, and the gradual amplification effect caused by the residue of the liquid can be avoided; and the assembly pipe 10 is cylindrically arranged, so that the assembly of the CPG carrier is conveniently realized by a machine, the automation is favorably realized, and the assembly efficiency is further improved.

Referring to fig. 3, a first transition pipe 14 is disposed between the first assembling location 11 and the second assembling location 12, the first assembling location 11 and the second assembling location 12 are communicated through the first transition pipe 14, and the inner diameter of the first transition pipe 14 is gradually decreased from the first assembling location 11 to the second assembling location 12, the CPG carrier includes a first carrier and a second carrier, the first carrier is located at the first assembling location 11, and the second carrier is located at the second assembling location 12. 2

In the present embodiment, a first transition pipe 14 is further disposed between the first assembling location 11 and the second assembling location 12, so as to realize the transition between the first assembling location 11 and the second assembling location 12, and make the liquid smoothly flow down along the inner wall of the transition pipe 14, thereby reducing the residue of the liquid.

Referring to fig. 4, the included angle formed by the inner wall of the first fitting location 11 and the inner wall of the first transition pipe 14 is α 1, 90 ° < α 1 < 180 °.

In the present embodiment, the included angle α 1 is in the range of 90 ° < α 1 < 180 °, that is, the inner wall of the first fitting location 11 and the inner wall of the first transition pipe 14 are arranged at an obtuse angle, and the inner wall of the first transition pipe 14 is contracted toward the center of the fitting pipe 10, so that the liquid flows down along the inner wall of the first fitting location 11 and the inner wall of the first transition pipe 14 under the action of gravity, thereby reducing the residue of the liquid.

Referring to fig. 5, a connection portion between an inner wall of the first assembling location 11 and an inner wall of the first transition pipe 14 is in a circular arc transition arrangement.

Optionally, the inner wall of the first assembling location 11 and the inner wall of the first transition pipe 14 adopt circular arc transition, that is, the inner wall of the first assembling location 11 and the inner wall of the first transition pipe 14 adopt smooth arc surfaces for smooth transition, and the smooth arc surfaces are favorable for the downward sliding of the liquid, so as to reduce the residue of the liquid at the joint of the inner wall of the first assembling location 11 and the inner wall of the first transition pipe 14. Specifically, the length of the arc is greater than the arc length corresponding to the sector with the radius of 1.5mm and the central angle of greater than 0.1 °, and the greater the arc length of the arc is, the more gradual the transition between the inner wall of the first fitting position 11 and the inner wall of the first transition pipe 14 is, and the more favorable the reduction of the liquid residue is.

Referring to fig. 3, a second transition pipe 15 is disposed between the second assembling station 12 and the third assembling station 13, the second assembling station 12 and the third assembling station 13 are communicated through the second transition pipe 15, and the inner diameter of the second transition pipe 15 is gradually decreased from the second assembling station 12 to the third assembling station 13, the CPG carrier further includes a third carrier 40, and the third carrier 40 is located at the third assembling station 13.

In this embodiment, the inner diameter of the second transition pipe 15 is gradually reduced from the second assembling location 12 to the third assembling location 13, which is beneficial for the liquid to smoothly flow down along the inner wall of the second transition pipe 15, thereby reducing the residue of the liquid and avoiding the waste of resources.

Referring to FIG. 4, the included angle formed by the second fitting location 12 and the inner wall of the second transition pipe 15 is defined as α 2, 90 ° < α 2 < 180 °.

In the embodiment, the included angle α 2 is in the range of 90 ° < α 2 < 180 °, that is, the second assembling location 12 is arranged at an obtuse angle with the inner wall of the second transition pipe 15, so that the liquid can flow down along the inner wall of the second transition pipe 15 under the action of gravity, and the residue of the liquid can be reduced.

Referring to fig. 5, the joint between the second assembling location 12 and the inner wall of the second transition pipe 15 is disposed in a circular arc transition manner.

Optionally, the second assembling location 12 and the second transition pipe 15 use a smooth arc surface for smooth transition, and the smooth arc surface is beneficial to the downward sliding of the liquid. Specifically, the length of the arc is greater than the arc length corresponding to the sector with the radius of 1.5mm and the central angle of greater than 0.1 degrees, and the arc length of the arc is larger and the transition is more gradual in a certain range, so that the liquid residue is reduced.

Specifically, the inner diameter of the second fitting site 12 is 0.1mm to 9.0mm, the height of the second fitting site 12 in the axial direction of the fitting pipe 10 is 0.5mm to 11.0mm, the inner diameter of the third fitting site 13 is 0.1mm to 5.0mm, and the height of the third fitting site 13 in the axial direction of the fitting pipe 10 is 0.5mm to 8.0 mm.

In this example, the second assembly site 12 is used for conventional DNA synthesis on a scale of 25-200 nmol; the third assembly site 13 is used for micro-DNA synthesis with a synthesis scale of 0-10 nmol. The second fitting site 12 has a size larger than that of the third fitting site 13, and facilitates fitting of the CPG carrier from the limiting tube 20 to the third fitting site 13 through the second fitting site 12.

Further, the diameter of the second carrier is not more than 8.8mm, the thickness of the second carrier is 0.5mm to 11.0mm, the diameter of the third carrier 40 is not more than 4.8mm, and the thickness of the third carrier 40 is 0.5mm to 8.0 mm.

The diameter of the second carrier is less than or equal to 8.8mm, and the thickness of the second carrier is between 0.5mm and 11.0m, so that DNA with the synthetic scale of between 25 and 200nmol is convenient to synthesize; the third carrier 40 has a diameter of 5.0mm or less and a thickness of 0.5mm to 8.0mm, thereby facilitating the synthesis of DNA on a scale of 0 to 10 nmol.

Further, the end of the assembling pipe 10 adjacent to the third assembling line 13 is a liquid outlet, and the liquid outlet is in a bevel or flat or sharp structure.

In this embodiment, an end of the assembling tube 10 adjacent to the third assembling line 13 is a liquid outlet, and the liquid outlet is in a bevel or flat or sharp structure. It can be understood that when the liquid outlet is of a bevel-opening and sharp-opening structure, the liquid can flow out conveniently, and the residue of the liquid on the inner wall can be reduced.

The DNA synthesis plate 100 further includes a frame 20 connecting the plurality of assembly tubes 10, and the frame 20 and the assembly tubes 10 are integrally formed.

In this embodiment, the frame 20 connects the plurality of assembling pipes 10, that is, the frame 20 and the assembling pipes 10 are integrally connected, so that the handling of the DNA synthesis plate 100 is facilitated.

Specifically, the frame 20 is a cuboid, the length of the frame 20 is 60mm to 140mm, the width of the frame 20 is 40mm to 110mm, and the height of the frame 20 is 8mm to 50 mm.

It can be understood that the arrangement density of the assembly tubes 10 determines the size of the frame 10, and the arrangement of the assembly tubes 10 is sparse, so that the size of the frame 10 is too large, which results in a large occupied space and is inconvenient for simultaneous synthesis of multiple DNAs, thereby reducing the synthesis efficiency; the assembling pipe 10 is densely arranged, so that the size of the frame 10 is too small, which is not beneficial for assembling the CPG carrier into the assembling pipe 10 by automatic assembling equipment. Preferably, the frame 10 has an outer dimension of 80mm to 136mm in length, 60mm to 98mm in width and 10mm to 40mm in height, and the size of the frame 10 is within this range, so as to facilitate the assembly of the CPG carrier.

The first embodiment is as follows:

1. design of 96-well DNA synthesis blank plate

The length of the hollow plate is 125.6mm, the width is 82mm, the height is 26mm, 96 holes are uniformly distributed in the plate body at equal intervals, the inner diameter of the circular hole part at the upper end of each hole is 6.0mm, and the height of the cavity part of the circular hole is 13.0 mm. The circular hole part at the upper end is gradually and smoothly transited to a second assembly position through a fillet (R is 1.5mm) with an included angle of more than R0.1 degrees to form an assembly position with the diameter of 2.5mm and the height of 5.0 mm; the second assembly position and the third assembly position are in gradual and smooth transition through a fillet (R is 1.5mm) with an included angle of more than R0.1 degrees, the transition gradually shrinks to 0.8mm, and finally the assembly position with the diameter of 0.8mm and the height of 3.0mm is formed. The sharp mouth part of the liquid outlet adopts a flat mouth design.

2. Preparation and assembly of CPG support:

2.1 preparation and Assembly of Synthesis Specification 25nmol CPG support

1) Raw materials: the loading capacity of the raw material CPG is 50 mu mol/g, and each CPG carrier with the synthetic amount of 25nmol contains 0.5mg of CPG; the molecular weight of the ultra-high molecular weight polyethylene (UHMW-PE) is 300 ten thousand.

2) Calculating the volume of the raw materials: the CPG carrier, each 2.5mm in diameter and 2.0mm in height, was 9.8125. mu.L in volume and was formulated as 1000 particles, having a total volume of about 9.8 mL.

3) Preparing CPG carrier sintering raw materials: weighing 500mg CPG, putting into a 10mL measuring cylinder, slowly adding UHMW-PE powder to 9.0mL scale, reversing and mixing uniformly for several times, then filling up UHMW-PE powder to 9.8mL position, reversing and mixing uniformly for later use.

4) Die filling: an aluminum die with a plurality of holes is adopted, the diameter of each hole is 2.5mm, the height of each hole is 2.0mm, the standby raw materials mixed with CPG and UHMW-PE powder are uniformly added into the holes, and the holes are compressed and vibrated to be flat.

5) And (3) sintering: sintering at 190 deg.C for 20min, cooling, and taking out the synthesized carrier to obtain CPG carrier with diameter of 2.5mm, height of 2.0mm and synthesis scale of 25 nmol.

6) Assembling: and assembling the CPG carrier with the diameter of 2.5mm and the height of 2.0mm into an upper second assembling position of the 96-hole empty plate by adopting full-automatic assembling equipment to obtain the 96-hole DNA synthetic plate with the synthetic scale of 25 nmol.

2.2 Synthesis Specification preparation and Assembly of 0.05nmol CPG support:

1) raw materials: the loading amount of the raw material CPG is 20 mu mol/g, and each CPG carrier with the synthetic amount of 0.05nmol contains 0.0025mg of CPG; the molecular weight of the ultra-high molecular weight polyethylene (UHMW-PE) is 300 ten thousand.

2) Calculating the volume of the raw materials: the volume of each CPG carrier with the diameter of 0.8mm and the height of 1.0mm is 0.5024 mu L, and the CPG carrier is prepared into 10000 particles, and the total volume is about 5.0 mL.

3) Preparing CPG carrier sintering raw materials: weighing 25mg of CPG, putting the CPG into a 10mL measuring cylinder, slowly adding UHMW-PE powder to 4.5mL scale, reversing and mixing uniformly for several times, then supplementing the UHMW-PE powder to 5.0mL position, reversing and mixing uniformly for later use.

4) Die filling: an aluminum die with a plurality of holes is adopted, the diameter of each hole is 0.8mm, the height of each hole is 1.0mm, the standby raw materials mixed with CPG and UHMW-PE powder are uniformly added into the holes, and the holes are compressed and vibrated to be flat.

5) And (3) sintering: sintering at 180 deg.C for 20min, cooling, and taking out the synthesized carrier to obtain CPG carrier with diameter of 0.8mm, height of 1.0mm and synthesis scale of 0.05 nmol.

6) Assembling: and assembling the CPG carrier with the diameter of 0.8mm and the height of 1.0mm to the third assembling position of the 96-hole empty plate by adopting full-automatic assembling equipment to obtain the 96-hole DNA synthetic plate with the synthetic scale of 0.05 nmol.

Example two:

1. design of 96-well DNA synthesis blank plate

The length of the hollow plate is 124.6mm, the width is 86mm, the height is 30mm, 96 holes are uniformly distributed on the plate body at equal intervals, the inner diameter of the circular hole part at the upper end of each hole is 6.2mm, and the height of the cavity part of the circular hole is 15.0 mm. The circular hole part at the upper end is gradually and smoothly transited to a second assembly position through an oblique angle with an included angle of 170 degrees to form an assembly position with the diameter of 3.0mm and the height of 6.0 mm; the second assembly position and the third assembly position are in gradual and smooth transition through an inclined angle with an included angle of 170 degrees, the gradual necking is gradually carried out to 1.0mm, and finally, the assembly position with the diameter of 1.0mm and the height of 4.0mm is formed. The sharp mouth part of the liquid outlet adopts a sharp mouth design.

2. Preparation and assembly of CPG support:

2.1 Synthesis Specification preparation of 50nmol CPG support:

1) raw materials: the loading capacity of the raw material CPG is 50 mu mol/g, and each CPG carrier with the synthetic amount of 50nmol contains 1mg of CPG; the molecular weight of the ultra-high molecular weight polyethylene (UHMW-PE) is 300 ten thousand.

2) Calculating the volume of the raw materials: each CPG carrier with a diameter of 3.0mm and a height of 4.0mm had a volume of 28.26. mu.L, and was formulated in 1000 pieces with a total volume of about 28.3 mL.

3) Preparing CPG carrier sintering raw materials: weighing 1g of CPG, putting the CPG into a 50mL measuring cylinder, slowly adding UHMW-PE powder to a scale of 25mL, reversing and uniformly mixing the mixture for a plurality of times, then supplementing the UHMW-PE powder to a position of 28.3mL, and reversing and uniformly mixing the mixture for later use.

4) Die filling: an aluminum die with a plurality of holes is adopted, the diameter of each hole is 3.0mm, the height of each hole is 4.0mm, the standby raw materials mixed with CPG and UHMW-PE powder are uniformly added into the holes, and the holes are compressed and vibrated to be flat.

5) And (3) sintering: sintering at 185 deg.C for 30min, cooling, and taking out; thus, a CPG support having a diameter of 3.0mm, a height of 4.0mm and a synthesis scale of 50nmol was obtained.

6) Assembling: and assembling the CPG carrier with the diameter of 3.0mm and the height of 4.0mm into an upper second assembling position of the 96-hole empty plate by adopting full-automatic assembling equipment to obtain the 96-hole DNA synthetic plate with the synthetic scale of 50 nmol.

2.2 Synthesis Specification preparation and Assembly of 2nmol CPG support:

1) raw materials: the loading capacity of the raw material CPG is 20 mu mol/g, and each CPG carrier with the synthetic amount of 2nmol contains 0.1mg of CPG; the molecular weight of the ultra-high molecular weight polyethylene (UHMW-PE) is 400 ten thousand.

2) Calculating the volume of the raw materials: the volume of each CPG carrier with the diameter of 1.0mm and the height of 1.2mm is 0.942 mu L, and the CPG carriers are prepared according to 10000 particles and have the total volume of 9.42 mL.

3) Preparing CPG carrier sintering raw materials: weighing 1.0mg CPG, placing into a 10mL measuring cylinder, slowly adding UHMW-PE powder to 9.0mL scale, reversing and mixing uniformly for several times, then filling up UHMW-PE powder to 9.4mL position, reversing and mixing uniformly for later use.

4) Die filling: an aluminum die with a plurality of holes is adopted, the diameter of each hole is 1.0mm, the height of each hole is 1.2mm, the standby raw materials mixed with CPG and UHMW-PE powder are uniformly added into the holes, and the holes are compressed and vibrated to be flat.

5) And (3) sintering: sintering at 180 deg.C for 20min, cooling, and taking out; thus, a CPG support having a diameter of 1.0mm, a height of 1.2mm and a synthesis scale of 2nmol was obtained.

6) Assembling: and assembling the CPG carrier with the diameter of 1.0mm and the height of 1.2mm to the third assembling position of the 96-hole empty plate by adopting full-automatic assembling equipment to obtain the 96-hole DNA synthetic plate with the synthetic scale of 2 nmol.

Example three:

1. design of 96-well DNA synthesis blank plate:

the length of the hollow plate is 126.0mm, the width is 86mm, the height is 33mm, 96 holes are uniformly distributed in the plate body at equal intervals, the inner diameter of the circular hole part at the upper end of each hole is 6.3mm, and the height of the cavity part of the circular hole is 18.0 mm. The circular hole part at the upper end is gradually and smoothly transited to a second assembly position through an oblique angle with an included angle of 160 degrees to form an assembly position with the diameter of 3.6mm and the height of 6.5 mm; the second assembly position and the third assembly position are in gradual and smooth transition through an oblique angle with an included angle of 160 degrees, the gradual transition is gradually closed to 2.0mm, and finally, the assembly position with the diameter of 2.0mm and the height of 4.5mm is formed. The sharp mouth part of the liquid outlet adopts an inclined opening design.

2. Preparation and assembly of CPG support:

2.1 Synthesis Specification preparation and Assembly of 200nmol CPG support:

1) raw materials: the loading capacity of the raw material CPG is 70 mu mol/g, and each CPG carrier with the synthetic amount of 50nmol contains CPG 2.85mg; the molecular weight of the ultra-high molecular weight polyethylene (UHMW-PE) is 500 ten thousand.

2) Calculating the volume of the raw materials: each CPG carrier with a diameter of 3.6mm and a height of 4.0mm had a volume of 40.69. mu.L, and was formulated in 1000 pieces with a total volume of about 40.7 mL.

3) Preparing CPG carrier sintering raw materials: weighing 2.85g of CPG, putting the CPG into a 50mL measuring cylinder, slowly adding UHMW-PE powder to 35mL scale, reversing and mixing uniformly for several times, then supplementing the UHMW-PE powder to 40.7mL position, reversing and mixing uniformly for later use.

4) Die filling: an aluminum die with a plurality of holes is adopted, the diameter of each hole is 3.6mm, the height of each hole is 4.0mm, the standby raw materials mixed with CPG and UHMW-PE powder are uniformly added into the holes, and the holes are compressed and vibrated to be flat.

5) And (3) sintering: sintering at 195 deg.C for 30min, cooling, and taking out; thus, a CPG support having a diameter of 3.6mm, a height of 4.0mm and a synthesis scale of 200nmol was obtained.

6) Assembling: and assembling the CPG carrier with the diameter of 3.6mm and the height of 4.0mm into an upper second assembling position of the 96-hole empty plate by adopting full-automatic assembling equipment to obtain the 96-hole DNA synthetic plate with the synthetic scale of 200 nmol.

2.2 Synthesis Specification preparation and Assembly of 5nmol CPG support:

1) raw materials: the loading capacity of the raw material CPG is 20 mu mol/g, and each CPG carrier with the synthesis amount of 5nmol contains 0.25mg of CPG; the molecular weight of the ultra-high molecular weight polyethylene (UHMW-PE) is 400 ten thousand.

2) Calculating the volume of the raw materials: the CPG carrier, each 2.0mm in diameter and 2.0mm in height, was 6.28. mu.L in volume and was formulated in 1000 particles, with a total volume of about 6.3 mL.

3) Preparing CPG carrier sintering raw materials: weighing 0.25g of CPG, putting the CPG into a 10mL measuring cylinder, slowly adding UHMW-PE powder to a scale of 5.8mL, reversing and uniformly mixing for several times, then supplementing the UHMW-PE powder to a position of 6.3mL, and reversing and uniformly mixing for later use.

4) Die filling: an aluminum die with a plurality of holes is adopted, the diameter of each hole is 2.0mm, the height of each hole is 2.0mm, the standby raw materials mixed with CPG and UHMW-PE powder are uniformly added into the holes, and the holes are compressed and vibrated to be flat.

5) And (3) sintering: sintering at 180 deg.C for 25min, cooling, and taking out; thus, a CPG support having a diameter of 2.0mm, a height of 2.0mm and a synthesis scale of 5nmol was obtained.

6) Assembling: and assembling the CPG carrier with the diameter of 2.0mm and the height of 2.0mm to the third assembling position of the 96-hole empty plate by adopting full-automatic assembling equipment to obtain the 96-hole DNA synthetic plate with the synthetic scale of 5 nmol.

The above only is the preferred embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structure changes made by the contents of the specification and the drawings under the inventive concept of the present invention, or the direct/indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A DNA synthesis plate, comprising:

2. The DNA synthesis plate according to claim 1, wherein a first transition pipe is provided between the first assembly site and the second assembly site, the first assembly site and the second assembly site are communicated through the first transition pipe, and the first transition pipe has an inner diameter gradually decreasing from the first assembly site to the second assembly site, and the CPG carrier comprises a first carrier and a second carrier, the first carrier being located at the first assembly site, and the second carrier being located at the second assembly site.

3. The DNA synthesis plate of claim 2, wherein the inner walls defining the first assembly positions form an angle with the inner walls of the first transition tube of α 1, 90 ° < α 1 < 180 °;

4. The DNA synthesis plate according to claim 2 or 3, wherein a second transition tube is provided between the second assembly site and the third assembly site, the second assembly site and the third assembly site communicating through the second transition tube, and the second transition tube has an inner diameter gradually decreasing from the second assembly site to the third assembly site, the CPG carrier further comprising a third carrier, the third carrier being located at the third assembly site.

5. The DNA synthesis plate of claim 4, wherein the second assembly site is defined to form an angle of α 2, 90 ° < α 2 < 180 °;

6. The DNA synthesis plate according to claim 5, wherein the inner diameter of the second fitting site is 0.1mm to 9.0mm, the height of the second fitting site in the axial direction of the fitting pipe is 0.5mm to 11.0mm, the inner diameter of the third fitting site is 0.1mm to 5.0mm, and the height of the third fitting site in the axial direction of the fitting pipe is 0.5mm to 8.0 mm.

7. The DNA synthesis plate according to claim 6, wherein the diameter of the second support is 8.8mm or less, the thickness of the second support is 0.5mm to 11.0mm, the diameter of the third support is 4.8mm or less, and the thickness of the third support is 0.5mm to 8.0 mm.

8. The DNA synthesis plate according to claim 7, wherein the end of the assembly tube adjacent to the third assembly site is a liquid outlet, and the liquid outlet has a bevel or flat or pointed configuration.

9. The DNA synthesis plate of claim 1, further comprising a frame connecting the plurality of assembly tubes, wherein the frame and the retainer tubes are integrally formed.

10. The DNA synthesis plate according to claim 9, wherein the frame is a rectangular parallelepiped, the length of the frame is 60mm to 140mm, the width of the frame is 40mm to 110mm, and the height of the frame is 8mm to 50 mm.