CN210314293U

CN210314293U - Small-size disconnect-type cell culture case

Info

Publication number: CN210314293U
Application number: CN201920733081.4U
Authority: CN
Inventors: 陈文凯; 林文平
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-05-21
Filing date: 2019-05-21
Publication date: 2020-04-14
Anticipated expiration: 2029-05-21

Abstract

The utility model discloses a small-sized separate cell culture box, which comprises a box body, a communicating pipe and a box body, wherein each wall of the box body is made of transparent materials and can be placed on a microscope objective table, and the side wall is connected with a spring and a baffle plate for fixing a cell culture dish, a culture plate or a culture bottle; the communicating pipe is connected out of the side wall of the box body and is jointed with the top wall of the box body, and the starting end and the tail end of the communicating pipe are both connected with a sealing ring and a filter screen and are used for communicating the box body with the gas environment in the box body; the casing of the box body is provided with a control panel and an alarm, the inside of the box body is provided with temperature, humidity and gas concentration sensors, and the inside of the box body is also provided with a temperature regulator, a humidity regulator, a ventilator, a carbon dioxide gas bottle and other equipment, so that the special external environment required by cell culture can be maintained. The utility model discloses rational utilization the finite space on the microscope objective table, provided a suitable external environment that can ensure cell growth, be favorable to the novel optimization platform of researcher real-time dynamic observation cell again.

Description

Small-size disconnect-type cell culture case

Technical Field

The utility model relates to a bioengineering field, concretely relates to small-size disconnect-type cell culture case.

Background

With the development and popularization of bioengineering technology, cell culture has become an experimental step generally operated in various biological laboratories, and meanwhile, a cell culture box has become a conventional instrument frequently used by researchers.

When observing the growth state of cells, an operator usually takes out a culture dish or a culture bottle from a large cell culture box and then places the culture dish or the culture bottle on a microscope objective table for observation, but the cells are separated from the original suitable growth environment, so the represented vitality state is greatly reduced, and particularly when the cell growth cycle and the differentiation state are dynamically observed for a long time, the operator needs to hold the culture dish by hand to frequently and repeatedly operate between the culture box and the microscope, and the cell is easily polluted and dead, even the culture box and other culture dishes are polluted.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned deficiencies of the prior art, it would be desirable to provide a cell culture incubator that is compact and separable and that can be placed on a microscope stage, according to an embodiment of the present invention.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a small-size disconnect-type cell culture case which characterized in that: the cell culture plate comprises a box body, a communicating pipe and a box body, wherein each wall of the box body is made of transparent materials and can be placed on an object stage of an inverted microscope, and the side wall of the box body is connected with a spring and a baffle plate for fixing a cell culture dish, a culture plate or a culture bottle; the transparent top cover of the box body is of a push-pull structure, a groove is formed in the periphery of the transparent top cover, a power switch is arranged on the shell of the side wall of the box body, an ultraviolet sterilizing lamp is connected inside the shell of the side wall of the box body and separated from the spring by a transparent partition plate, and a pressure balance valve, a filter screen and a sealing ring are arranged on the side wall of the box body; the communicating pipe is connected out of the side wall of the box body and is jointed with the top wall of the box body, and the starting end and the tail end of the communicating pipe are both connected with the sealing ring and the filter screen. The communicating pipe is a heat-insulating and gas-tight hose, is used for conveying gas generated in the box body to the box body, and can be used for balancing the temperature, the humidity and various gas concentrations in the box body and the box body.

Further, the box shell is provided with a control panel and an alarm, the control panel is composed of a display screen, a keyboard and an intelligent control unit, and the display screen, the keyboard and the alarm are connected to the intelligent control unit respectively.

Furthermore, the shell of the box body is connected with an air conduit and a pressure balance valve, and a closed heat-insulating layer is arranged in the box body.

Furthermore, the box is internally provided with an ultraviolet sterilizing lamp, an air filter, a temperature controller, a humidity controller, a ventilator and a carbon dioxide storage bottle, and is also provided with a gas concentration sensor, a humidity sensor and a temperature sensor, and the intelligent control unit is used for adjusting the gas concentration sensor, the humidity sensor and the temperature sensor, so that the intelligent control unit has the function of automatically controlling the gas environment in the closed heat insulation layer.

Further, the ventilator is communicated with the carbon dioxide storage bottle through a carbon dioxide conduit.

Further, the gas flow direction of the pressure balance valve is from the inside of the box or the inside of the box to the outside.

Compared with the prior art, the beneficial effects of the utility model are that:

1. through the setting of a small-size disconnect-type incubator, divide into function case, communicating pipe and the transparent thing box three major parts of putting with the cell culture case, the transparent thing box of putting can place on microscope objective table for build the suitable external environment that can ensure cell growth, be favorable to the novel optimization platform of researcher real-time dynamic observation cell again.

2. Through the setting of spring and baffle in the transparent thing box of putting, fix culture dish or blake bottle, can improve stability and the security that culture dish or blake bottle placed.

3. Through the setting of communicating pipe and pressure balance valve, can balance transparent temperature, humidity, gas concentration and the gas pressure of putting in box and the function box in real time.

Drawings

FIG. 1 is a front view of the structure of the present invention;

FIG. 2 is a sectional view of the structure of the case of the present invention;

FIG. 3 is a cross-sectional view of the structure of the box body of the present invention;

FIG. 4 is a top view of the box structure of the present invention;

fig. 5 is a side view of the box structure of the present invention.

In the figure: 1-box, 2-communicating pipe, 3-box, 4-alarm, 5-control panel, 6-closed heat preservation layer, 7-air conduit, 8-air filter screen, 91-sealing ring, 92-sealing ring, 101-first ultraviolet sterilizing lamp, 102-second ultraviolet sterilizing lamp, 111-first pressure balancing valve, 112-second pressure balancing valve, 12-gas concentration sensor, 13-humidity sensor, 14-temperature sensor, 15-air filter, 16-carbon dioxide conduit, 17-temperature controller, 18-humidity controller, 19-ventilator, 20-carbon dioxide storage bottle, 21-rubber pad, 22-transparent top cover, 23-transparent partition board, 24-filter screen, 25-switch, 26-spring, 27-baffle, 28-transparent bottom plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope. Based on the embodiments of the present invention, those skilled in the art should equally replace some of the technical features of the embodiments, and any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Referring to fig. 1-5, a small-sized separable cell culture chamber according to a preferred embodiment of the present invention includes a chamber body 3, a communicating tube 2, and a chamber body 1. Each wall of the box body 3 is made of transparent materials and can be placed on an object stage of the inverted microscope, and the side wall is connected with a spring 26 and a baffle plate 27 for fixing a cell culture dish, a culture plate or a culture bottle. The transparent top cover 22 of the box body 3 is a push-pull structure, a groove is arranged on the periphery of the transparent top cover, a power switch 25 is arranged on the outer shell of the side wall of the box body 3, the second ultraviolet sterilizing lamp 102 is connected inside the box body 3 and is separated from the spring 26 by a transparent partition plate 23, and the side wall of the box body 3 is provided with a second pressure balance valve 112, a filter screen 24 and a sealing ring 92.

The communicating pipe 2 is connected out of the side wall of the box body 3 and is jointed with the top wall of the box body 1, the starting end and the tail end of the communicating pipe are both connected with the sealing ring 92 and the filter screen 24, the communicating pipe is a heat-insulating and gas-tight hose which is used for conveying gas generated in the box body 1 to the box body 3 and balancing the temperature, the humidity and various gas concentrations in the box body 1 and the box body 3.

Wherein, 1 shell of box is equipped with control panel 4 and siren 5, control panel 4 comprises display screen, keyboard and intelligent control unit, and display screen, keyboard and siren 5 are connected to intelligent control unit respectively.

Wherein, the shell of the box body 1 is connected with an air conduit 7 and a first pressure balance valve 111, and a closed heat preservation layer 6 is arranged inside the box body 1.

The box body 1 is internally provided with a first ultraviolet sterilizing lamp 101, an air filter 15, a temperature controller 17, a humidity controller 18, a ventilator 19, a carbon dioxide storage bottle 20, a gas concentration sensor 12, a humidity sensor 13 and a temperature sensor 14, and an intelligent control unit analyzes received data and outputs a control instruction, so that the box body has the function of automatically controlling the gas environment in the closed heat-insulating layer 6.

Wherein the ventilator 19 inside the box body 1 is communicated with the carbon dioxide storage bottle 20 through the carbon dioxide conduit 16.

The gas flow direction of the first pressure balance valve 111 on the box body 1 is from the inside to the outside of the box, and the gas flow direction of the second pressure balance valve 112 on the box body 3 is from the inside to the outside of the box.

Claims

1. The utility model provides a small-size disconnect-type cell culture case which characterized in that: the box comprises a box body (3), a communicating pipe (2) and a box body (1), wherein each wall of the box body (3) is made of transparent materials, and a spring (26) and a baffle (27) are connected to the side wall of the box body; the transparent top cover (22) of the box body (3) is of a push-pull type structure, grooves are formed in the periphery of the transparent top cover, a power switch (25) is arranged on the outer shell of the side wall of the box body (3), a second ultraviolet sterilizing lamp (102) is connected inside the box body and is separated from the spring (26) through a transparent partition plate (23), and a second pressure balance valve (112), a filter screen (24) and a sealing ring (92) are arranged on the side wall of the box body (3); the communicating pipe (2) is connected out of the side wall of the box body (3) and is jointed with the top wall of the box body (1), and the starting end and the tail end of the communicating pipe are both connected with the sealing ring (92) and the filter screen (24).

2. The compact detached cell incubator of claim 1, wherein: the box (1) shell is equipped with control panel (4) and siren (5), control panel (4) comprise display screen, keyboard and intelligent control unit, display screen, keyboard and siren (5) are connected to intelligent control unit respectively.

3. A compact split cell incubator as recited in claim 1, wherein: the box body (1) shell has connect air pipe (7) and first pressure balance valve (111), box body (1) inside is equipped with seals heat preservation (6).

4. A compact split cell incubator as recited in claim 3, wherein: the box body (1) is internally provided with a first ultraviolet sterilizing lamp (101), an air filter (15), a temperature controller (17), a humidity controller (18), a ventilator (19) and a carbon dioxide storage bottle (20), and is also provided with a gas concentration sensor (12), a humidity sensor (13) and a temperature sensor (14), and the box body is adjusted by an intelligent control unit and has the function of automatically controlling the gas environment in the sealed heat-insulating layer (6).

5. The compact detached cell incubator of claim 4, wherein: the ventilator (19) is in communication with the carbon dioxide storage bottle (20) via a carbon dioxide conduit (16).

6. A compact split cell incubator as recited in claim 3, wherein: the gas flow direction of the first pressure balance valve (111) is from the inside of the box to the outside, and the gas flow direction of the second pressure balance valve (112) is from the inside of the box to the outside.