CN210287375U

CN210287375U - Cell culture plate

Info

Publication number: CN210287375U
Application number: CN201920988997.4U
Authority: CN
Inventors: 孟荣华
Original assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Current assignee: Taipingyang Kangtai Scientific Apparatus Jinan Co ltd
Priority date: 2019-06-27
Filing date: 2019-06-27
Publication date: 2020-04-10
Anticipated expiration: 2029-06-27

Abstract

The utility model relates to a cell culture plate, which comprises a base plate, a plurality of unit holes arranged on the top surface of the base plate and a cover body covered above the base plate. The top surface of the base plate is provided with a boss, the top surface of the boss is provided with a concave groove, and the plurality of unit holes are alternately dispersed in the groove. The inner wall of the cover body is opposite to the side wall of the boss. The isolation groove regions are arranged among the unit holes, so that the problem that the current cell culture plate is easy to cause cross contamination is solved.

Description

Cell culture plate

Technical Field

The utility model relates to a cell culture household utensils field, concretely relates to cell culture board.

Background

The cell culture technology is widely applied to the fields of medical science, biological research and application. Among them, the cell culture dish is the most commonly used vessel for containing liquid culture medium, so that the cells can grow adherently or in suspension in the liquid. The cell culture plate is used as an integrally formed multi-hole type dish device, a plurality of unit holes are arranged to be respectively used for containing culture solution, and cultivators place the multi-hole cell culture plate in the same cell culture box, so that the same cell can grow and propagate in a uniform and balanced environment. However, the existing porous cell culture plate has the problems that the culture solution is easy to overflow and cross contamination is easy to cause in the using process.

SUMMERY OF THE UTILITY MODEL

To the problem that current cell culture board caused cross contamination easily, the utility model provides a cell culture board of novel structure, it has set up isolation groove district between each unit hole, has solved the problem that the culture board caused cross contamination easily in shifting and the operation effectively.

The utility model provides a technical scheme that its technical problem adopted is: a cell culture plate comprises a base plate, a plurality of unit holes arranged on the top surface of the base plate, and a cover body covering the base plate. The top surface of the base plate is provided with a boss, the top surface of the boss is provided with a concave groove, and the plurality of unit holes are alternately dispersed in the groove. The inner wall of the cover body is opposite to the side wall of the boss.

In some embodiments, the joint of the side wall and the bottom wall of each unit hole is provided with an arc surface, and two ends of the arc surface are tangent to the side wall and the bottom wall respectively.

In some embodiments, the bore groove of the cell bore is a conical bore with an inner diameter tapering from the outer port to the bottom surface.

In some embodiments, a vertically upward extending flange is provided on the top surface of the boss and around the groove. On the interior diapire of lid, and at least in the inboard of part relative lateral wall of lid is equipped with respectively along the card strip of vertical direction extension. When the cover body is arranged on the boss, the inner wall surface of the clamping strip is in contact with the outer wall surface of the flange, and pressure can be applied to the flange by means of deformation of the clamping strip. Preferably, a contact surface between the clip strip and the flange is in a line-surface contact relationship, and in particular, an inner wall surface of the clip strip may be set to be a curved surface.

In some embodiments, the locking strips correspond to the left and right side walls of the cover body. The cover body can be in translational sliding relative to the boss along the front-back direction. The outer side wall of the flange is provided with a groove, and the clamping strip can be switched to move into and out of the groove in the process that the cover body slides back and forth relative to the boss. The front-back width of the groove is larger than that of the clamping strip. A gap can be formed between the inner wall of the clamping strip and the bottom wall of the groove. The card strip can be selected to be plate-shaped or cylindrical. When the clamping strip is plate-shaped, in order to ensure that the clamping strip can smoothly move in and out of the groove, the front side edge and the rear side edge of the clamping strip are both set to be curved surfaces.

The utility model has the advantages that: the isolation groove regions are arranged among the unit holes, so that the problem that the current cell culture plate is easy to cause cross contamination is solved.

Drawings

Fig. 1 is a schematic top view of the first embodiment of the present patent.

Fig. 2 is a schematic top view of the second embodiment of the present invention.

Fig. 3 is a schematic diagram of a modified structure of the patent.

Fig. 4 is a cross-sectional structural view of a transverse cross-section of the structure shown in fig. 3.

Fig. 5 is a schematic diagram of a further improved structure of the patent.

1 base plate, 2 bosses, 21 flanges, 22 grooves, 31 and 31' unit holes, 32 grooves, 4 cover bodies and 41 clamping teeth

Detailed Description

The drawings in the specification show the structure, ratio, size, etc. only for the purpose of matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and not for the purpose of limiting the present invention, so the present invention does not have the essential meaning in the art, and any structure modification, ratio relationship change or size adjustment should still fall within the scope covered by the technical content disclosed in the present invention without affecting the function and achievable purpose of the present invention. Meanwhile, the terms "upper", "lower", "front", "rear", "middle", and the like used in the present specification are for the sake of clarity only, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof are also considered to be the scope of the present invention without substantial changes in the technical content.

As shown in FIGS. 1 to 5, a cell culture plate includes a base plate 1, a plurality of unit wells 31, 31' provided on the top surface of the base plate 1, and a cover 4 covering the base plate 1. The top surface of the substrate 1 is provided with a boss 2, the top surface of the boss 2 is provided with a concave groove 32, and the plurality of unit holes 31 and 31' are alternately dispersed in the groove 32. The inner wall of the cover 4 is opposite to the side wall of the boss 2.

Among the above-mentioned embodiment, the type groove that sets up can be at the in-process of implementing the operation to each unit hole, and as the buffer area, because the notch in each unit hole is the parallel and level with the notch in type groove, so the culture solution can not cause the influence to other unit holes after falling into the type groove during the operation, can effectively solve cross contamination's problem between the unit hole. Moreover, the washing operation can be carried out by the aid of the groove, and the washing operation cannot affect the unit holes.

As shown in fig. 2, the bore groove of the unit bore 31' is a tapered bore with an inner diameter gradually decreasing from the outer port to the bottom surface. The arrangement can save the use amount of the culture solution and simultaneously facilitate the cell extraction from the unit hole.

In the embodiment of the present patent, preferably, the junction of the side wall and the bottom wall of each unit hole is set to be an arc surface, and two ends of the arc surface are tangent to the side wall and the bottom wall respectively. This arrangement can prevent cells from being trapped in the corners where the side walls and the bottom wall meet (in a typical cell, the corners between the side walls and the bottom wall are 90 degrees, so that cells can be easily trapped therein), and facilitates the removal of all cells from the cell wells.

As shown in fig. 3 and 4, a vertically upward extending flange 21 is provided on the top surface of the boss 2 at the periphery of the groove 32. On the inner bottom wall of the lid 4, and inside the opposite side walls of the lid, there are provided vertically extending locking strips 41, respectively. When the lid body 4 is placed on the boss 2, the inner wall surface of the clip strip 41 is in contact with the outer wall surface of the flange 21, and pressure can be applied to the flange 21 by plastic deformation of the clip strip 41. The arrangement can ensure the fastening performance of the cover body after being combined with the substrate (or the lug boss), and is favorable for ensuring the stability in stacking. After the clamping strip is matched with the flange, a clearance space can be formed between the inner side wall surface of the cover body and the side wall surface of the flange, a clearance can be formed between the inner bottom wall of the cover body and the top surface of the flange, or a through hole or a through groove is formed in the wall body of the flange (if the groove mentioned later is set as the through groove), so that the air supply exchange effect can be ensured. Preferably, a contact surface between the clip strip 41 and the flange 21 is in a line-surface contact relationship, and specifically, as shown in fig. 4, an inner wall surface of the clip strip 41 may be set to be a curved surface. In the embodiment shown in fig. 4, the cover body is covered above the boss from top to bottom, so the lower end of the clamping strip is a curved surface tilted outwards, and the middle part of the clamping strip is a curved surface tangent to the side wall surface of the flange.

As shown in fig. 5, the locking strips 41 correspond to the left and right side walls of the cover 4. The cover 41 is capable of sliding in a forward-backward direction with respect to the boss 2. The outer side wall of the flange 21 is provided with a groove 22, and the clamping strip 41 can be switched to move into and out of the groove 22 in the process that the cover body 4 slides back and forth relative to the boss 2. The front-back width of the groove 22 is larger than the front-back width of the clamping strip 41. A gap can be formed between the inner wall of the clamping strip 41 and the bottom wall of the groove 22. The card strip 41 may be selected to be plate-shaped or cylindrical. When the clamping strip is plate-shaped, in order to ensure that the clamping strip can smoothly move in and out of the groove, the front side edge and the rear side edge of the clamping strip are both set to be curved surfaces.

In the above embodiment, after the cover is placed on the boss from top to bottom, the cover is slid backward relative to the substrate, so that the cover and the boss are fixedly combined together, as shown in fig. 5. Therefore, in the embodiment shown in fig. 5, the vertical distance between the front and rear inner wall surfaces of the lid body is larger than the length of the boss in the front-rear direction.

The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. The present invention can be modified in many ways without departing from the spirit and scope of the present invention, and those skilled in the art can modify or change the embodiments described above without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims

1. A cell culture plate comprises a base plate, a plurality of unit holes arranged on the top surface of the base plate, and a cover body covering the base plate; the method is characterized in that: the top surface of the substrate is provided with a boss, the top surface of the boss is provided with a concave groove, and the plurality of unit holes are alternately dispersed in the groove; the inner wall of the cover body is opposite to the side wall of the boss.

2. The cell culture plate of claim 1, wherein: the hole groove of the unit hole is a conical hole cavity with the inner diameter gradually reduced from the outer port to the bottom surface.

3. A cell culture plate according to claim 1 or 2, wherein: the lateral wall and the diapire linking department in each unit hole all set up to the cambered surface, and the both ends of cambered surface are tangent with lateral wall and diapire respectively.

4. A cell culture plate according to claim 1 or 2, wherein: a flange which extends vertically and upwards is arranged on the periphery of the groove on the top surface of the boss; the inner bottom wall of the cover body and at least the inner sides of the partial opposite side walls of the cover body are respectively provided with a clamping strip extending along the vertical direction; when the cover body is arranged on the boss, the inner wall surface of the clamping strip is in contact with the outer wall surface of the flange, and pressure can be applied to the flange by means of plastic deformation of the clamping strip.

5. The cell culture plate of claim 4, wherein: the contact surface between the clamping strip and the flange is in line-surface contact relation.

6. The cell culture plate of claim 4, wherein: a plurality of clamping strips are arranged on the inner side of each side wall corresponding to the cover body.

7. The cell culture plate of claim 4, wherein: the clamping strips are correspondingly arranged on the left side wall and the right side wall of the cover body; the cover body can be in translational sliding relative to the boss along the front and back directions; the outer side wall of the flange is provided with a groove, and the clamping strip can be switched to move into and out of the groove in the process that the cover body slides back and forth relative to the boss; the front-back width of the groove is larger than that of the clamping strip.

8. The cell culture plate of claim 7, wherein: the clamping strip is cylindrical.

9. The cell culture plate of claim 7, wherein: the clamping strip is plate-shaped, and the front side edge and the rear side edge of the clamping strip are both set to be curved surfaces.