CN210269342U

CN210269342U - Water environment DNA extraction element

Info

Publication number: CN210269342U
Application number: CN201920884328.2U
Authority: CN
Inventors: 董姗姗; 章嫡妮; 张振华
Original assignee: Nanjing Institute of Environmental Sciences MEE
Current assignee: Nanjing Institute of Environmental Sciences MEE
Priority date: 2019-06-13
Filing date: 2019-06-13
Publication date: 2020-04-07
Anticipated expiration: 2029-06-13

Abstract

The utility model discloses a water environment DNA extraction device, which comprises a fixed bottle and a bracket, wherein the fixed bottle is arranged on the bracket and is provided with a plurality of feed inlets, the bracket is connected with a mixing structure, and an automatic grouping component is arranged below the mixing structure; the utility model relates to a water environment DNA technical field, this water environment DNA extraction element, moreover, the steam generator is simple in structure, high durability and convenient use, measure the solution quantity of at every turn testing through deciding the bottling, carry out autogiration through mixed structure, carry out intensive mixing to internal solution, save the experimenter physical power on the one hand, increase efficiency of software testing on the one hand, through automatic grouping subassembly, will decide in the bottle in the mixed solution automatic shunt to each reagent bottle, can directly carry out the centrifugation, further accelerated test efficiency, realized saving the experimenter physical power through the cooperation of above structure, the simplified operation step, the purpose of increase efficiency of software testing.

Description

Water environment DNA extraction element

Technical Field

The utility model relates to a water environment DNA technical field specifically is a water environment DNA extraction element.

Background

As a technique, the purpose of analyzing eDNA is to obtain taxonomic and genetic functional information about the species to which the DNA belongs in these environmental samples. To put it more colloquially, scientists extract eDNA from environmental samples in order to analyze which species these DNAs belong to, respectively, and thus verify which species are present in the corresponding environment. The purpose of eDNA is in agreement with traditional animal and plant classification studies.

The DNA detection of the water environment can help people to evaluate the water environment so as to obtain related parameters such as pollutant types, microbial community distribution conditions and the like, and has high practical significance and economic value, so that the DNA detection has great significance for collecting and detecting the DNA of the water environment.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art, the utility model provides a water environment DNA extraction element has solved current DNA extraction element and has made the reagent of smallclothes many through grouping, it is even to rock through various additives of filling in to the smallclothes reagent, can examine after the centrifugation, this step is many through manual operation, not only waste time, operating procedure has been increased, people's dropwise add reagent has slowly reduced the experimental efficiency, and when the manpower rocks the reagent, can't thoroughly mix it usually, the problem of experimental efficiency has more been reduced.

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: a water body environment DNA extraction device comprises a fixed bottle and a bracket, wherein the fixed bottle is arranged on the bracket and is provided with a plurality of feed inlets, the bracket is connected with a mixing structure, and an automatic grouping assembly is arranged below the mixing structure;

the hybrid structure includes: the driving machine, the external tooth groove structure and the driving gear;

the fixed bottle is movably arranged in the circular through hole and can rotate in the circular through hole, the lower end of the outer wall surface of the fixed bottle is provided with an external tooth groove structure, the driving machine is arranged on the support, the driving end of the driving machine is provided with a driving gear, the driving gear is meshed with the external tooth groove structure, the upper end of the fixed bottle is open, and the inner upper end of the fixed bottle is provided with a filtering assembly;

the automatic grouping component includes: the reagent bottle comprises a rotary joint, a shunt, a plurality of shunt tubes with the same structure and a plurality of reagent bottles with the same structure;

rotary joint connects in surely adorning the bottle lower part, the shunt is connected on rotary joint, and is a plurality of the shunt tubes is connected in the shunt below, and is a plurality of the reagent bottle is installed on the support, and is located a plurality of the shunt tubes below, and is located a plurality of all install control flap on the shunt tubes.

Preferably, the filter assembly comprises: the filter plate comprises a plurality of mounting plates with the same structure, a plurality of filter plates and a plurality of inserted rods with the same structure;

a plurality of the mounting panel is annular array and installs in deciding bottled inner wall upper end, and all seted up the jack on it, and is a plurality of the inserted bar is annular array and installs in the filter plate lower wall, the filter plate passes through a plurality of inserted bar cartridges in a plurality of on the mounting panel.

Preferably, the fixed bottle is of a cylindrical cavity structure, the upper end of the fixed bottle is open, and a sealing door is hinged on the opening.

Preferably, the edge of the filter plate is of an inner groove structure.

Preferably, the side wall surface of the fixed bottle is provided with an observation window, the observation window is provided with protective glass, and a dial gauge is engraved on the protective glass.

Preferably, a plurality of shock pads are installed at four corners of the bottom of the support.

Advantageous effects

The utility model provides a water environment DNA extraction element. The method has the following beneficial effects: the water environment DNA extraction device has simple structure and convenient use, measures the solution dosage of each test through the fixed bottle, automatically rotates through the mixing structure, fully mixes the internal solution, saves the physical strength of experimenters on the one hand, increases the test efficiency on the other hand, automatically shunts the mixed solution in the fixed bottle into each reagent bottle through the automatic grouping component, can be directly centrifuged, further accelerates the test efficiency, realizes the purposes of saving the physical strength of the experimenters, simplifying the operation steps and increasing the test efficiency through the structural cooperation, effectively solves the problems that the existing DNA extraction device mostly divides the mixed solution into small parts through grouping, uniformly shakes by filling various additives into the small parts of the reagent, can be inspected after centrifugation, mostly adopts manual operation, wastes time and increases the operation steps, the experiment efficiency is slowly reduced by people dropping the reagent, and when the reagent is shaken by manpower, the reagent can not be uniformly mixed generally, so that the experiment efficiency is further reduced.

Drawings

FIG. 1 is a schematic structural diagram of a water environment DNA extraction device according to the present invention.

FIG. 2 is a schematic diagram of a side view structure of the water environment DNA extraction device of the present invention.

FIG. 3 is a schematic view of the top view structure of the water environment DNA extraction device of the present invention.

In the figure: 1. bottling by fixed amount; 2. a support; 3. a feed inlet; 4. an external tooth groove structure; 5. a driver; 6. a driving gear; 7. a rotary joint; 8. a flow divider; 9. a shunt tube; 10. a control valve; 11. mounting a plate; 12. inserting a rod; 13. filtering the plate; 14. a sealing door; 15. an observation window; 16. a shock-absorbing pad.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

All the electrical components in the present application are connected with the power supply adapted to the electrical components through a wire, and an appropriate controller and an appropriate encoder should be selected according to actual conditions to meet control requirements, and specific connection and control sequences should be obtained.

Example (b): according to the attached drawings 1-3 of the specification, the water body environment DNA extraction device mainly comprises a fixed bottle 1 and a support 2, wherein the support 2 is an installation main body and is used for providing supporting force, the fixed bottle 1 is installed on the support 2, a plurality of feed inlets 3 are formed in the fixed bottle 1, in the specific implementation process, additives are injected into the fixed bottle 1 through the feed inlets 3, a mixing structure is connected to the fixed bottle 1 on the support 2, and an automatic grouping assembly is arranged below the mixing structure;

as can be seen from fig. 1-3 of the specification, the hybrid structure comprises: a driving machine 5, an external tooth groove structure 4 and a driving gear 6;

the support 2 is provided with a circular through hole, the fixed charging bottle 1 is movably arranged in the circular through hole and can rotate in the circular through hole, the fixed charging bottle 1 is meshed with the driving gear 6 through the external tooth socket structure 4 below the fixed charging bottle 1, and in the specific implementation process, the driving gear 5 is started to drive the driving end to rotate, so that the driving gear 6 on the driving gear is driven to rotate, and is meshed with the external tooth socket structure 4, the fixed charging bottle 1 is driven to rotate in the bearing on the circular through hole, and the solution in the fixed charging bottle 1 is fully mixed in a shaking manner;

as can be seen from fig. 1-3 of the specification, the automatic grouping assembly comprises: the reagent bottle comprises a rotary joint 7, a shunt 8, a plurality of shunt tubes 9 with the same structure and a plurality of reagent bottles with the same structure;

in the specific implementation process, the shunt 8 is kept still in the rotating process of the fixed bottle 1 through the rotary joint 7, after shaking, the mixed solution enters the shunt 8 by opening the main valve and then enters a plurality of reagent bottles through a plurality of shunt tubes 9, and whether the solution is injected into a single reagent bottle or not and the bearing capacity of the single reagent bottle can be controlled through a control valve 10 on the shunt tubes 9;

in conclusion, the water environment DNA extraction device is simple in structure and convenient to use, the solution dosage of each test is measured through the fixed bottle 1, the solution dosage is automatically rotated through the mixing structure to fully mix the internal solution, the physical strength of experimenters is saved, the test efficiency is improved, the mixed solution in the fixed bottle 1 is automatically distributed into each reagent bottle through the automatic grouping assembly, the centrifugation can be directly carried out, the test efficiency is further accelerated, the purposes of saving the physical strength of the experimenters, simplifying the operation steps and improving the test efficiency are realized through the structural cooperation, the problems that the existing DNA extraction device is mostly divided into small parts of reagents through grouping, various additives in the small parts of reagents are uniformly shaken and can be checked after centrifugation are effectively solved, the steps are mostly operated by manpower, the time is wasted, the operation steps are added, the experiment efficiency is slowly reduced by dropping the reagent by people, and the reagent cannot be uniformly mixed normally when being shaken by manpower, so that the experiment efficiency is further reduced.

Preferably, as further shown in fig. 2-3 of the specification, the filter assembly comprises: a plurality of mounting plates 11 with the same structure, a filter plate 13 and a plurality of inserted rods 12 with the same structure;

through passing the jack on a plurality of mounting panels 11 with inserted bar 12, place filter plate 13 on mounting panel 11, fix its week, can take off filter plate 13 simultaneously and clear up, filter the sample water through the filter screen in the filter plate 13, when reaching filterable purpose, save time easy dismounting.

Preferably, the fixed bottle 1 is a cylindrical cavity structure, and the upper end of the fixed bottle is open, and a sealing door 14 is hinged on the opening, and the sealing door 14 is used for carrying the feeding hole 3 and can be opened and closed for cleaning and trimming.

Preferably, the edge of the filter plate 13 is provided with an inner groove structure to prevent impurities from being retained on the outer wall surface.

Preferably, the side wall surface of the fixed bottle 1 is provided with an observation window 15, the observation window 15 is provided with a piece of protective glass, and a dial gauge is engraved on the protective glass, so that observation is facilitated.

Preferably, a plurality of shock absorbing pads 16 are arranged at four corners of the bottom of the support 2 to reduce the shaking of the equipment.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation. The use of the phrase "comprising one of the elements does not exclude the presence of other like elements in the process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A water environment DNA extraction device comprises a fixed bottle (1) and a support (2), and is characterized in that the fixed bottle (1) is arranged on the support (2), a plurality of feed inlets (3) are formed in the fixed bottle, a mixing structure is connected with the fixed bottle (1) on the support (2), and an automatic grouping assembly is arranged below the mixing structure;

the hybrid structure includes: a driving machine (5), an external tooth groove structure (4) and a driving gear (6);

the fixed bottle is characterized in that a circular through hole is formed in the support (2), the fixed bottle (1) is movably arranged in the circular through hole and can rotate in the circular through hole, an external tooth groove structure (4) is arranged at the lower end of the outer wall surface of the fixed bottle (1), the driving machine (5) is arranged on the support (2), a driving gear (6) is arranged at the driving end of the driving machine, the driving gear (6) is meshed with the external tooth groove structure (4), the upper end of the fixed bottle (1) is opened, and a filtering assembly is arranged at the inner upper end of the fixed bottle;

the automatic grouping component includes: the reagent bottle comprises a rotary joint (7), a shunt (8), a plurality of shunt tubes (9) with the same structure and a plurality of reagent bottles with the same structure;

rotary joint (7) are connected in deciding bottling (1) lower part, shunt (8) are connected on rotary joint (7), and are a plurality of shunt tubes (9) are connected in shunt (8) below, and are a plurality of the reagent bottle is installed on support (2), and is located a plurality of shunt tubes (9) below, and is located a plurality of all install control flap (10) on shunt tubes (9).

2. The device for extracting DNA from an aqueous environment of claim 1, wherein the filter assembly comprises: a plurality of mounting plates (11) with the same structure, filter plates (13) and a plurality of inserted rods (12) with the same structure;

a plurality of mounting panel (11) are the annular array and install in deciding bottle (1) inner wall upper end, and all seted up the jack on it, and are a plurality of inserted bar (12) are the annular array and install in filter plate (13) lower wall, filter plate (13) are in a plurality of through a plurality of inserted bar (12) cartridge on mounting panel (11).

3. The device for extracting DNA from water body environment as claimed in claim 1, wherein the stationary bottle (1) is a cylindrical cavity structure, and has an upper opening hinged with a sealing door (14).

4. The device for extracting DNA from water body environment as claimed in claim 2, wherein the edge of the filter plate (13) is of an inner groove structure.

5. The device for extracting DNA from a water environment according to claim 1, wherein an observation window (15) is opened on a side wall surface of the fixed bottle (1), the observation window (15) is provided with a cover glass, and a scale is engraved on the cover glass.

6. The device for extracting DNA from a water environment as claimed in claim 1, wherein a plurality of shock absorbing pads (16) are arranged at four corners of the bottom of the bracket (2).