CN210237644U - Perfusion type culture system - Google Patents
Perfusion type culture system Download PDFInfo
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- CN210237644U CN210237644U CN201921087262.0U CN201921087262U CN210237644U CN 210237644 U CN210237644 U CN 210237644U CN 201921087262 U CN201921087262 U CN 201921087262U CN 210237644 U CN210237644 U CN 210237644U
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Abstract
Provided is a perfusion culture system, comprising: a culture compartment containing biological material; the circulating supply loop comprises a liquid storage tank and a first power source, the liquid storage tank is used for storing culture solution and is communicated with the culture cabin, the first power source is arranged on a pipeline which enables the liquid storage tank to be communicated with the culture cabin, and the culture solution circularly flows between the liquid storage tank and the culture cabin under the driving of the first power source; the new liquid supplement branch comprises a new liquid tank and a second power source, the new liquid tank is used for storing fresh culture liquid and communicated with the liquid storage tank, the second power source is arranged on a pipeline for communicating the new liquid tank with the liquid storage tank, and the second power source can supplement the fresh culture liquid to the liquid storage tank. The perfusion type culture system is provided with the new liquid supplement branch, so that fresh culture liquid can be supplemented to the liquid storage tank, the supply quality and quantity of the culture liquid are guaranteed, the liquid storage tank has the functions of storing the culture liquid, receiving the supplement culture liquid and supplying the culture liquid, and the pipeline structure of the perfusion type culture system is simplified.
Description
Technical Field
The utility model relates to the technical field of biological medical treatment, and in particular to perfusion culture system.
Background
Perfusion culture is a cell or cell microsphere culture mode in which biological materials such as cells or cell microspheres and the like and a culture solution are added into a reactor together, and part of the culture solution is continuously taken out and simultaneously new culture solution is continuously perfused in the processes of cell or cell microsphere growth and product formation.
CN107723236A discloses a perfusion culture system, which has a liquid storage part that supplies culture liquid to a culture compartment and temporarily stores the culture liquid. The perfusion culture system does not have a culture solution recovery system and a culture solution replenishment system.
CN106754356A discloses a perfusion culture system, which has a three-dimensional culture device, a culture solution recovery storage tank, a culture solution supply tank for storing culture solution and supplying the culture solution to the whole system, an impurity filtration system, and an automatic culture solution supply system. A broth recovery storage tank is located downstream of the impurity filtration system and is used to temporarily store the filtered broth. The culture solution automatic supply system is used for supplying fresh culture solution to the culture solution recovery storage tank.
The culture solution recovery storage tank, the culture solution supply tank, the impurity filtering system and the three-dimensional culture device are arranged on the circulating culture loop. Nutrient substances in the culture solution are consumed by cells in the three-dimensional culture device, the filtered culture solution flows back to the culture solution recovery storage tank, and the culture solution automatic supply system automatically supplies fresh culture solution to the culture solution recovery storage tank due to the change of the pH value. Along with the increase of the number of times of the circulating culture, more and more liquid (cells only consume nutrient substances but not liquid) can be contained in the culture liquid recovery storage tank, the dynamic balance of a circulating loop is finally broken, and the liquid overflows, so that manual operation is needed to extract a part of culture liquid in the culture liquid recovery storage tank at regular intervals, the manual intervention is increased, and the automation and high yield of the cell culture cannot be realized.
This perfusion culture system adopts culture solution supply tank and the culture solution recovery storage jar that has temporary storage function of establishing ties each other, all need carry out the benefit of fresh nutrient solution in culture solution recovery storage jar in advance before the culture solution gets into the culture solution supply tank that has the supply function, the circulation circuit of culture solution has not only been prolonged, the culture solution in the storage jar is retrieved to the suction power source that leads to needing higher power extracts, and lead to the complete machine structure complicacy, tedious, the cost is higher, the crisscross complicacy of conduit line, need great installation space.
Therefore, it is highly desirable to provide a perfusion culture system that can supply, supplement, and store a culture solution with a simple configuration.
SUMMERY OF THE UTILITY MODEL
The present invention has been made in view of the above-described state of the art. An object of the utility model is to provide a perfusion culture system of simple structure, it can realize the supply of culture solution, replenishment and storage simultaneously.
Provided is a perfusion culture system, comprising:
a culture compartment containing biological material;
a circulation supply circuit including a liquid storage tank that stores a culture solution and communicates with the culture chamber, and a first power source that is provided in a pipe that communicates the liquid storage tank with the culture chamber and drives the culture solution to circulate between the liquid storage tank and the culture chamber, the culture solution in the liquid storage tank being driven by the first power source to be supplied to the culture chamber, and the culture solution flowing out of the culture chamber being received by the liquid storage tank;
the new liquid supplementing branch is connected with the circulating supply loop and comprises a new liquid tank and a second power source, the new liquid tank is used for storing fresh culture liquid and communicated with the liquid storage tank, the second power source is arranged on a pipeline enabling the new liquid tank to be communicated with the liquid storage tank, and the second power source can supplement the fresh culture liquid to the liquid storage tank.
In at least one embodiment, the perfusion culture system further includes a waste liquid recovery branch, the waste liquid recovery branch is a branch connected to the circulation supply loop, the waste liquid recovery branch includes a waste liquid tank and a third power source, the waste liquid tank is communicated with the liquid storage tank, the third power source is disposed in a pipeline communicating the waste liquid tank with the liquid storage tank, and the third power source can recover the culture liquid in the liquid storage tank to the waste liquid tank.
In at least one embodiment, the volume of the fresh broth replenished by the fresh broth replenishing branch is equal to the volume of the broth recovered by the spent broth recovery branch.
In at least one embodiment, one or more of the first, second, and third power sources is a peristaltic pump.
In at least one embodiment, the perfusion culture system further comprises a control module, wherein an electric control valve is arranged on a pipeline for communicating the new liquid tank with the liquid storage tank and a pipeline for communicating the waste liquid tank with the liquid storage tank, and the control module controls the opening and closing of the electric control valve and/or the opening degree of the electric control valve.
In at least one embodiment, the perfusion culture system further comprises a detection device for detecting one or more of oxygen content, pH value, lactose content, glucose content of the culture fluid in the reservoir tank.
In at least one embodiment, the perfusion culture system further comprises a gas filtering device, the gas filtering device is arranged on a pipeline of the culture cabin communicated with the liquid storage tank, and the gas filtering device can introduce oxygen and carbon dioxide into the culture solution.
In at least one embodiment, the perfusion culture system further comprises a control module, the gas filtering device is controlled by the control module, the detection data of the detection device can be transmitted to the control module, and the control module controls the gas filtering device according to the data detected by the detection device.
In at least one embodiment, the culture compartment contains cell microspheres, and the culture compartment further has a cross-linking agent port for allowing a cross-linking agent to enter and exit the culture compartment, and a cleaning agent port for allowing a cleaning agent to enter and exit the culture compartment.
In at least one embodiment, the culture compartment has a culture solution inlet and a culture solution outlet, and the culture solution inlet, the culture solution outlet, the cross-linking agent interface, and the cleaning agent interface are all provided with filter screens.
The technical scheme at least has the following technical effects:
the perfusion type culture system is provided with a new liquid supplement branch, so that a liquid storage tank can be supplemented with a fresh culture liquid, and the supply quality and quantity of the culture liquid are ensured. In addition, the liquid storage tank has the functions of storing the culture solution, receiving the supplemented culture solution and supplying the culture solution, multiple purposes are realized by one liquid storage tank, the circulation loop of the culture solution is simplified, the culture solution in the liquid storage tank can be extracted by adopting a power source with smaller power, the pipeline structure of the perfusion type culture system is simplified, and the required installation space is smaller.
The technical scheme can also have the following technical effects:
the waste liquid recovery branch road can retrieve the culture solution in the liquid storage tank, provides accommodation space for fresh culture solution, and the culture solution that supplements the branch road cooperation with new liquid realizes this perfusion culture system is changed.
The culture solution in the circular supply loop forms a dynamic balance system, when the volume of the supplement solution is equal to that of the recovery solution, the culture solution is maintained at a proper volume, and the volume is maintained, so that the phenomenon that the culture solution in the loop overflows and is not wasted redundantly is avoided, and the phenomenon that the culture solution is too little to influence dynamic culture is avoided.
Fresh culture solution in the new liquid tank is supplemented to the liquid storage tank, and the culture solution in the liquid storage tank is output to the waste liquid tank, so that the culture can be directly supplemented to the culture cabin or directly recovered from the culture cabin. This can prevent the biomaterial from being subjected to large impact and disturbance when the fresh culture solution is replenished and the culture solution is recovered, and is advantageous for protecting the structure of the biomaterial from being damaged.
The arrangement of the electric control valve improves the automation degree of the perfusion type culture system and is beneficial to accurately controlling the input quantity and the output quantity of the culture solution.
The detection device can monitor the state of the culture solution in the perfusion culture system in real time. Thereby detection device can realize the instant detection to the culture solution in the culture cabin is stored to the liquid storage pot. The liquid storage tank is also independent of the culture cabin, which is beneficial to avoiding the interference of biological materials during detection and realizing effective detection.
Drawings
FIG. 1 is a schematic diagram of a culture fluid circulation circuit in one embodiment of a perfusion culture system provided by the present disclosure.
FIG. 2 is a schematic diagram of the flow lines for cross-linking and cleaning agents of the perfusion culture system of FIG. 1.
Description of reference numerals:
the device comprises a circulation supply loop 1, a liquid storage tank 11, a first power source 12, a new liquid supplement branch 2, a new liquid tank 21, a second power source 22, a waste liquid recovery branch 3, a waste liquid tank 31, a third power source 32, a culture cabin 4, a cross-linking agent tank 41, a fourth power source 42, a detection device 5, a cleaning agent tank 51, a fifth power source 52, a gas filtering device 6, a cross-linking agent interface 7a, a cleaning agent interface 7b, a culture liquid inlet 7c, a culture liquid outlet 7d, a stirring blade 81, a driving motor 82 and a filter screen 9.
Detailed Description
Exemplary embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the detailed description is only intended to teach one skilled in the art how to practice the invention, and is not intended to exhaust all possible ways of practicing the invention, nor is it intended to limit the scope of the invention.
As shown in fig. 1, the present disclosure provides a perfusion culture system including a culture compartment 4, a circulation supply circuit 1, a fresh liquid supplement branch 2, and a waste liquid recovery branch 3.
The culture compartment 4 contains biological material and is used for culturing biological material, such as biological cells, biological tissue, biological organs, etc.
The circulation supply circuit 1 includes a liquid reservoir 11 and a first power source 12, the liquid reservoir 11 stores a culture solution for culturing the biological material and communicates with the culture chamber 4, and the first power source 12 is provided in a pipeline that communicates the liquid reservoir 11 with the culture chamber 4. The culture solution in the liquid storage tank 11 circularly flows between the liquid storage tank 11 and the culture compartment 4 under the driving of the first power source 12. The culture liquid in the liquid reservoir tank 11 is driven by the first power source 12 to be supplied to the culture compartment 4, and the culture liquid flowing out from the culture compartment 4 is received by the liquid reservoir tank 11.
Specifically, the culture chamber 4 has a culture solution inlet 7c and a culture solution outlet 7d, and the culture solution inlet 7c and the culture solution outlet 7d are both connected with a pipeline. The culture liquid in the culture chamber 4 flows out through the culture liquid outlet 7d and flows into the liquid storage tank 11 through the pipeline. The culture solution in the liquid storage tank 11 flows out through the pipeline and flows into the culture chamber 4 through the culture solution inlet 7 c.
The new liquid replenishing branch 2 is a branch of the circulation supply circuit 1 and includes a new liquid tank 21 and a second power source 22, the new liquid tank 21 stores a fresh culture liquid and is communicated with the liquid storage tank 11, and the second power source 22 is provided in a pipeline for communicating the new liquid tank 21 with the liquid storage tank 11. The second power source 22 can supply fresh culture solution to the liquid reservoir tank 11.
The new liquid supplement branch 2 can supplement the fresh culture liquid to the liquid storage tank 11, so that the supply quality and quantity of the culture liquid are ensured. In addition, the liquid storage tank 11 has the functions of storing the culture solution, receiving the supplemented culture solution and supplying the culture solution, the multiple purposes are realized by one liquid storage tank 11, the circulation loop of the culture solution is simplified, the culture solution in the liquid storage tank can be extracted by adopting a power source with smaller power, the pipeline structure of the perfusion type culture system is simplified, and the required installation space is smaller.
The perfusion culture system provides a dynamic culture solution which can be monitored in real time, can realize the long-term culture of biological materials, such as three-dimensional cell structures, in vitro, improves the culture efficiency and reduces the culture cost.
The waste liquid recovery branch 3 is a branch of the circulation supply loop 1 and comprises a waste liquid tank 31 and a third power source 32, the waste liquid tank 31 is communicated with the liquid storage tank 11, and the third power source 32 is arranged on a pipeline for communicating the waste liquid tank 31 with the liquid storage tank 11. The third power source 32 can recover the culture liquid in the liquid reservoir 11 to the waste liquid reservoir 31.
When the quality of the culture solution in the liquid storage tank 11 does not meet the standard, the culture solution in the liquid storage tank 11 is recovered by the waste solution recovery branch 3, and then the fresh culture solution is supplemented to the liquid storage tank 11 by the new solution supplementing branch 2.
The waste liquid recovery branch 3 can recover the culture solution in the liquid storage tank 11, provides a containing space for the fresh culture solution, and is matched with the new solution supplement branch 2 to realize the culture solution replacement of the perfusion type culture system.
As shown in FIG. 1, the arrow M indicates the flow of the culture medium in the circulation supply circuit 1, the arrow F indicates the flow of the culture medium in the fresh liquid replenishing branch passage 2, and the arrow F indicates the flow of the culture medium in the waste liquid collecting branch passage 3.
The fresh culture solution in the new solution tank 21 is supplemented to the solution storage tank 11, and the culture solution in the solution storage tank 11 is output to the waste solution tank 31, so as to avoid directly supplementing the culture compartment 4 or directly recycling the culture compartment 4. This can prevent the biomaterial from being subjected to large impact and disturbance when the fresh culture solution is replenished and the culture solution is recovered, and is advantageous for protecting the structure of the biomaterial from being damaged.
Preferably, the perfusion culture system may further include a control module, and the pipeline through which the new liquid tank 21 communicates with the liquid storage tank 11, and the pipeline through which the waste liquid tank 31 communicates with the liquid storage tank 11 may be provided with an electronic control valve, and the electronic control valve may be opened and closed under the control of the control module, or may have a predetermined opening degree.
This improves the degree of automation of the perfusion culture system and is beneficial to accurately controlling the input and output of the culture solution.
The perfusion culture system may further comprise a detection device 5, wherein the detection device 5 is used for detecting the content of the culture solution in the liquid storage tank 11, such as oxygen content, pH value, lactose content and glucose content. The detection means 5 may detect one or more of the above-mentioned indicators. The detection device 5 can monitor the state of the culture solution in the perfusion culture system in real time.
The detection means 5 may be connected to the control module described above so as to transmit detection data to the control module. The control module can control the switches of the waste liquid recovery branch 3, the new liquid supplement branch 2 and the circulation supply loop 1, the flow rate and the like according to the detection data of the detection device 5. For example, nutrient content of the culture solution is judged according to glucose content so as to control the supplement of fresh culture solution, and metabolite content is judged according to lactose content so as to control the recovery of used culture solution.
It should be understood that the new liquid supplement branch 2 and the waste liquid recovery branch 3 are not only activated according to the above data, but also the waste liquid recovery branch 3 and the new liquid supplement branch 2 may be activated, for example, when the oxygen content, the pH value, and the like are not satisfactory.
The liquid storage tank 11 stores the culture solution in the culture compartment 4 so that the detection device 5 can realize the instant detection of the culture solution. The liquid storage tank 11 is also independent of the culture cabin 4, which is beneficial to avoiding the interference of biological materials during detection and realizing effective detection.
Preferably, the volume of the culture solution supplemented by the fresh solution supplementing branch 2 is equal to the volume of the culture solution recovered by the waste solution recovering branch 3, so that the input and output of the culture solution of the perfusion culture system are balanced.
The culture solution in the circular supply loop forms a dynamic balance system, when the volume of the supplement solution is equal to that of the recovery solution, the culture solution is maintained at a proper volume, and the volume is maintained, so that the phenomenon that the culture solution in the loop overflows and is not wasted redundantly is avoided, and the phenomenon that the culture solution is too little to influence dynamic culture is avoided.
Specifically, for example, the second power source 22 and the third power source 32 may be peristaltic pumps, the flow rates of the second power source 22 and the third power source 32 are preset to be the same, and the control module controls the working time of the second power source 22 and the working time of the third power source 32 to be the same. Or, the above-mentioned electric control valves are arranged on the pipeline connecting the new liquid tank 21 and the liquid storage tank 11, and the pipeline connecting the waste liquid tank 31 and the liquid storage tank 11, and the control module adjusts the above-mentioned two electric control valves in real time according to the information fed back by the flow detection unit.
The electric control valve can be a one-way valve, a flow valve and the like, and can be an electromagnetic valve.
The perfusion culture system can also comprise a gas filtering device 6, wherein the gas filtering device 6 is arranged on a pipeline for communicating the culture cabin 4 with the liquid storage tank 11, and the gas filtering device 6 can introduce oxygen and carbon dioxide into the culture solution so as to control the oxygen concentration of the culture solution and adjust CO of the culture solution2And (4) concentration.
The gas filtering means 6 may be connected to a control module, which may control the gas filtering means 6 according to the data detected by the detecting means 5. For example, when the data transmitted by the detection means 5 indicate that the oxygen content of the culture broth does not meet the standards, the gas filtration means 6 is supplemented with oxygen. When the data transmitted by the detection device 5 indicate that the pH value of the culture solution does not meet the standard, the gas filtration device 6 is supplemented with CO2。
The gas filtering device 6 may be a gas filtering device of the prior art, such as the gas filtering device of CN 106754356A.
The perfusion culture system can culture cell microspheres.
The interior of the culture compartment 4 may contain cell microspheres, which may be, for example, three-dimensional hydrogel microspheres, which may be cell-containing hydrogel formed by uniformly mixing autoclaved hydrogel (e.g., containing sodium alginate and gelatin) with a cell suspension of a specific concentration.
Stirring blades 81 (such as the blades of a three-blade wheel) may be provided inside the culture compartment 4. Outside the culture compartment 4, for example, below the culture compartment 4, there may be a driving motor 82, and a driving shaft of the driving motor 82 is extended into the culture compartment 4 and connected to the stirring blade 81.
The stirring blades 81 can be driven to stir the cell microspheres in the culture chamber 4, so that the cell microspheres are in full and uniform contact with the culture solution to exchange nutrition with the culture solution, and dynamic culture of cells is realized.
The perfusion culture system cultures the cell microspheres, provides a three-dimensional environment similar to an in-vivo environment for in-vitro culture of cells, is favorable for obtaining a cell growth space with higher unit density, accelerates the cell growth speed, and realizes culture and amplification of cells with higher efficiency and high characteristics.
As shown in fig. 2, the perfusion culture device may further include a cross-linking agent supply assembly and a cleaning agent supply assembly. In the figure, the arrow J indicates the flow direction of the crosslinking agent, and the arrow Q indicates the flow direction of the cleaning agent.
The crosslinker supply assembly may include a crosslinker tank 41 and a fourth power source 42, the crosslinker tank 41 storing crosslinker (e.g., CaCl)2) The culture compartment 4 is provided with a cross-linking agent port 7a and is communicated with a cross-linking agent tank 41 through the cross-linking agent port 7 a. The fourth power source 42 is provided in a line connecting the crosslinking agent tank 41 and the culture chamber 4, and is used for supplying the crosslinking agent in the crosslinking agent tank 41 to the culture chamber 4 and extracting the crosslinking agent in the culture chamber 4.
The fourth power source 42 may be first turned on to introduce the cross-linking agent into the culture compartment 4, and then the cell microspheres may be put into the culture compartment 4, and the cells in the cell microspheres may be cross-linked under the action of the cross-linking agent, so as to maintain the morphology of the cell microspheres. After the crosslinking is completed, the fourth power source 42 is turned on to draw the crosslinking agent in the culture compartment 4 back to the crosslinking agent tank 41.
The cleaning agent supply assembly may include a cleaning agent tank 51 and a fifth power source 52, the cleaning agent tank 51 stores a cleaning agent, and the cultivation compartment 4 is provided with a cleaning agent port 7b and communicates with the cleaning agent tank 51 through the cleaning agent port 7 b. The fifth power source 52 is provided in a pipe line that communicates the cleaning agent tank 51 with the culture chamber 4, and is used for supplying the cleaning agent in the cleaning agent tank 51 to the culture chamber 4 and for pumping out the cleaning agent in the culture chamber 4.
After the fourth power source 42 is used to draw out the cross-linking agent, the fifth power source 52 may be turned on to supply the cleaning agent to the culture compartment 4, and after the cleaning agent is applied to the culture compartment 4 for a predetermined time (e.g., 5 minutes), the fifth power source 52 may be turned on to draw the cleaning agent in the culture compartment 4 back to the cleaning agent tank 51.
The cross-linking agent interface 7a, the cleaning agent interface 7b, the culture solution inlet 7c and the culture solution outlet 7d can be provided with filter screens 9 (for example, the aperture is 100 micrometers), and the filter screens 9 can limit the cell microspheres from diffusing to the outside of the culture chamber 4.
The first power source 12, the second power source 22, the third power source 32, the fourth power source 42, and the fifth power source 52 may be peristaltic pumps.
The following describes possible modes of operation of the perfusion culture system.
When the detection device 5 detects the culture solution and the lactose content is too high and the glucose content is too low, the electric control valve arranged on the waste liquid recovery branch 3 is opened, the third power source 32 sucks the culture solution in the liquid storage tank 11 to the waste liquid tank 31, and the electric control valve arranged on the waste liquid recovery branch 3 is closed when the third power source 32 works for a preset time or the flow detection unit detects a preset flow. Then the electric control valve arranged in the new liquid supplementing branch 2 is opened, the second power source 22 pumps the fresh culture solution in the new liquid tank 21 to the liquid storage tank 11, and the electric control valve arranged in the new liquid supplementing branch 2 is closed when the second power source 22 works for a preset time or the flow detection unit detects a preset flow.
It should be understood that the above embodiments are exemplary only, and are not intended to limit the present invention. Various modifications and alterations of the above-described embodiments may be made by those skilled in the art in light of the teachings of the present invention without departing from the scope thereof.
Claims (10)
1. A perfusion culture system, comprising:
a culture compartment (4), the culture compartment (4) containing biological material;
a circulation supply circuit (1), wherein the circulation supply circuit (1) comprises a liquid storage tank (11) and a first power source (12), the liquid storage tank (11) stores a culture solution and is communicated with the culture compartment (4), the first power source (12) is arranged on a pipeline which enables the liquid storage tank (11) to be communicated with the culture compartment (4) and drives the culture solution to circularly flow between the liquid storage tank (11) and the culture compartment (4), the culture solution in the liquid storage tank (11) is driven by the first power source (12) to be supplied to the culture compartment (4), and the culture solution flowing out of the culture compartment (4) is received by the liquid storage tank (11);
the new liquid supplementing branch (2) is connected to the circulation supply loop (1), the new liquid supplementing branch (2) comprises a new liquid tank (21) and a second power source (22), the new liquid tank (21) is used for storing fresh culture liquid and communicated with the liquid storage tank (11), the second power source (22) is arranged on a pipeline enabling the new liquid tank (21) to be communicated with the liquid storage tank (11), and the second power source (22) can supplement the fresh culture liquid to the liquid storage tank (11).
2. A perfusion culture system according to claim 1, further comprising a waste liquid recovery branch (3), wherein the waste liquid recovery branch (3) is a branch connected to the circulation supply loop (1), the waste liquid recovery branch (3) comprises a waste liquid tank (31) and a third power source (32), the waste liquid tank (31) is communicated with the liquid storage tank (11), the third power source (32) is provided in a pipeline communicating the waste liquid tank (31) with the liquid storage tank (11), and the third power source (32) can recover the culture liquid in the liquid storage tank (11) to the waste liquid tank (31).
3. A perfusion culture system according to claim 2, wherein the volume of the fresh culture fluid replenished by the fresh fluid replenishing branch (2) is equal to the volume of the culture fluid recovered by the waste fluid recovering branch (3).
4. A perfusion culture system according to claim 2, wherein one or more of the first, second, and third power sources (12, 22, 32) are peristaltic pumps.
5. A perfusion culture system according to claim 2, further comprising a control module, wherein the pipeline of the new liquid tank (21) communicating with the liquid storage tank (11) and the pipeline of the waste liquid tank (31) communicating with the liquid storage tank (11) are both provided with an electric control valve, and the control module controls the opening and closing of the electric control valve and/or the opening degree of the electric control valve.
6. A perfusion culture system according to claim 1, further comprising a detection device (5), the detection device (5) being configured to detect one or more of an oxygen content, a pH value, a lactose content, a glucose content of the culture fluid within the fluid reservoir (11).
7. A perfusion culture system according to claim 6, further comprising a gas filtration device (6), wherein the gas filtration device (6) is provided in a pipeline connecting the culture compartment (4) and the liquid storage tank (11), and wherein the gas filtration device (6) is capable of introducing oxygen and carbon dioxide into the culture solution.
8. A perfusion culture system according to claim 7, further comprising a control module, the gas filtration device (6) being controlled by the control module, detection data of the detection device (5) being transmittable to the control module, the control module controlling the gas filtration device (6) in accordance with data detected by the detection device (5).
9. A perfusion culture system according to claim 1, wherein the culture compartment (4) contains cell microspheres, the culture compartment (4) further has a cross-linking agent port (7a) and a cleaning agent port (7b), the cross-linking agent port (7a) is used for the cross-linking agent to enter and exit the culture compartment (4), and the cleaning agent port (7b) is used for the cleaning agent to enter and exit the culture compartment (4).
10. A perfusion culture system according to claim 9, wherein the culture compartment (4) has a culture fluid inlet (7c) and a culture fluid outlet (7d), and the culture fluid inlet (7c), the culture fluid outlet (7d), the cross-linking agent port (7a), and the cleaning agent port (7b) are provided with a filter screen (9).
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| CN113528328A (en) * | 2021-07-07 | 2021-10-22 | 韶关学院 | Bacterial biofilm culture apparatus |
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| CN113528328A (en) * | 2021-07-07 | 2021-10-22 | 韶关学院 | Bacterial biofilm culture apparatus |
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