CN210235761U

CN210235761U - Immunofluorescence staining kit

Info

Publication number: CN210235761U
Application number: CN201921123891.4U
Authority: CN
Inventors: Huiqiong Wu; 吴慧琼; Yi Zhang; 张翼; Fei Fang; 方飞
Original assignee: Suzhou Tianru Biological Technology Co ltd
Current assignee: Suzhou Tianru Biological Technology Co ltd
Priority date: 2019-07-18
Filing date: 2019-07-18
Publication date: 2020-04-03
Anticipated expiration: 2029-07-18

Abstract

The utility model discloses an immunofluorescence staining kit, including kit body and a plurality of reagent pipes of setting in the kit body, the intraductal detection liquid that is equipped with of reagent, the top of reagent pipe extends to the top of kit body, the bottom fixed mounting of kit body has the fixed case, fixed mounting has the montant on the bottom inner wall of fixed case, the sliding sleeve is equipped with the diaphragm on the montant, one side fixed mounting of diaphragm has the clamp plate, the through-hole has been seted up on one side inner wall of fixed case, one side of clamp plate is run through the through-hole and is extended to outside the fixed case, the equal fixed mounting in bottom four corners of clamp plate has the triangle piece, and two relative triangle piece symmetries set up. The utility model discloses simple structure, convenient operation, be convenient for fix the kit on the workstation firm fast, effectually avoid bumping on causing the intraductal detection liquid of reagent to spill the workstation because of the mistake, satisfy the use needs, be favorable to using.

Description

Immunofluorescence staining kit

Technical Field

The utility model relates to a biological medicine equipment technical field especially relates to an immunofluorescence dyeing kit.

Background

An immunofluorescence staining detection method is an important technology in life science and medical research, which mainly utilizes the specific combination of antigen and antibody to position specific protein in cells or tissues, the detection method utilizes the principle of fluorescence, has high detection sensitivity, can position specific protein in sub-cell level, and can perform co-positioning detection on two or more proteins in cells or tissues, plays an important role in life science and medical research, the kit is required to be used in the detection process, the existing kit comprises a kit body and a plurality of reagent tubes arranged in the kit body, detection liquid is arranged in the reagent tubes, the top ends of the reagent tubes extend to the upper part of the kit body, researchers place the kit on a workbench, take out cell creepers from a culture medium and place the cell creepers into the reagent tubes to react with the detection liquid, the researchers observed the reaction conditions to obtain the research results.

But current kit not convenient to stable place at the workstation, the researcher touches the kit easily by mistake and drives the kit and rock at the in-process that detects to lead to the intraductal detection liquid of reagent to spill on the workstation, the clearance of being not convenient for when the extravagant cost brings inconvenience for the researcher, therefore we have provided an immunofluorescence dyeing kit and have been used for solving above-mentioned problem.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing an immunofluorescence staining kit.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

an immunofluorescence staining kit comprises a kit body and a plurality of reagent tubes arranged in the kit body, wherein detection liquid is arranged in the reagent tubes, the top ends of the reagent tubes extend to the upper part of the kit body, a fixed box is fixedly arranged at the bottom of the kit body, vertical rods are fixedly arranged on the inner wall of the bottom of the fixed box, a transverse plate is sleeved on the vertical rods in a sliding manner, a pressing plate is fixedly arranged on one side of the transverse plate, a through hole is formed in the inner wall of one side of the fixed box, one side of the pressing plate penetrates through the through hole and extends out of the fixed box, triangular blocks are fixedly arranged at four corners of the bottom of the pressing plate, two opposite triangular blocks are symmetrically arranged, suckers are fixedly arranged at four corners of the bottom of the fixed box, four L-shaped tubes are arranged in the fixed box, two opposite L-shaped tubes are symmetrically arranged, and, the bottom ends of the L-shaped pipes extend into the corresponding suckers, the four L-shaped pipes are arranged in one-to-one correspondence with the four triangular blocks, a piston is hermetically and slidably sleeved in the L-shaped pipe, a cross rod is fixedly arranged on one side of the piston close to the corresponding triangular block, a plurality of first springs are fixedly arranged between the bottom of the transverse plate and the inner wall of the bottom of the fixed box, one side of the transverse rod close to the corresponding triangular block is rotatably provided with a roller which is in rolling contact with the inclined surface of the corresponding triangular block, the top of the transverse rod is provided with a rectangular hole, the same fixed rod is fixedly arranged between the inner walls at the two sides of the rectangular hole, fixed mounting has four bracing pieces on the bottom inner wall of fixed case, and four bracing pieces and four horizontal pole one-to-ones set up, and the top of bracing piece extends to the rectangular hole that corresponds, and the bracing piece slip cap is established on the dead lever that corresponds, and fixed mounting has a plurality of second springs between one side inner wall that the piston was kept away from to one side that the corresponding piston was kept away from to the bracing piece and the rectangular hole.

Preferably, the top of the transverse plate is provided with a through hole, and the side wall of the through hole is in sliding connection with the outer side of the vertical rod.

Preferably, the piston is fixedly sleeved with a sealing ring, and the outer side of the sealing ring is in sliding connection with the side wall of the corresponding L-shaped pipe.

Preferably, two sides of the pressing plate are respectively connected with the inner walls of the two sides of the through hole in a sliding mode.

Preferably, the number of the second springs in the same rectangular hole is two to four, and the two to four second springs in the same rectangular hole are arranged on the inner wall of one side of the rectangular hole far away from the corresponding piston at equal intervals.

Preferably, the number of the first springs is two to four, and the two to four first springs are arranged on the inner wall of the bottom of the fixed box at equal intervals.

Compared with the prior art, the beneficial effects of the utility model are that:

by the arrangement of the reagent kit body, the reagent tube, the fixing box, the vertical rod, the transverse plate, the pressing plate, the through hole, the triangular block, the sucking disc, the L-shaped tube, the piston, the transverse rod, the first spring, the idler wheel, the rectangular hole, the fixing rod, the supporting rod, the second spring, the perforation and the sealing ring are matched, when the reagent kit is used, the pressing plate is pressed downwards to drive the four triangular plates to move downwards through the transverse plate and compress the first spring, the triangular plates extrude the corresponding idler wheel to drive the transverse rod to move towards the direction close to the piston, the transverse rod drives the corresponding piston to move and compress the second spring, when the piston moves, a gas is pushed out to be discharged into the sucking disc through the corresponding L-shaped tube, then the gas is discharged through the sucking disc, at the moment, the fixing box is placed on the workbench, the four sucking discs extrude the workbench and deform to be adsorbed on the workbench by, the sucking disc can reset after taking place deformation, not hard up clamp plate, first spring force resets and drives four set squares rebound through the diaphragm and resets, second spring force resets and drives the piston through the horizontal pole that corresponds and remove in the L venturi tube, fixed cover is equipped with the sealing washer on the piston, the sealing washer has good leakproofness, the piston is bled in to the sucking disc through the L venturi tube that corresponds, make the inside negative pressure reinforcing of sucking disc, thereby make firm the adsorbing on the workstation of kit, and be convenient for press the clamp plate exhaust air downwards and relieve the absorption.

The utility model discloses simple structure, convenient operation, be convenient for fix the kit on the workstation firm fast, effectually avoid bumping on causing the intraductal detection liquid of reagent to spill the workstation because of the mistake, satisfy the use needs, be favorable to using.

Drawings

FIG. 1 is a schematic structural diagram of an immunofluorescent staining kit provided by the present invention;

FIG. 2 is a schematic sectional view of the stationary box of FIG. 1;

FIG. 3 is a schematic structural view of portion A of FIG. 2;

FIG. 4 is a top view of the vertical bar and the horizontal plate of FIG. 2.

In the figure: the kit comprises a kit body 1, reagent tubes 2, a fixing box 3, a vertical rod 4, a transverse plate 5, a pressing plate 6, a through hole 7, a triangular block 8, a sucking disc 9, an L-shaped tube 10, a piston 11, a transverse rod 12, a first spring 13, a roller 14, a rectangular hole 15, a fixing rod 16, a supporting rod 17, a second spring 18, a perforation 19 and a sealing ring 20.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-4, an immunofluorescence staining kit comprises a kit body 1 and a plurality of reagent tubes 2 arranged in the kit body 1, wherein detection liquid is arranged in the reagent tubes 2, the top ends of the reagent tubes 2 extend to the upper side of the kit body 1, a fixed box 3 is fixedly arranged at the bottom of the kit body 1, a vertical rod 4 is fixedly arranged on the inner wall of the bottom of the fixed box 3, a transverse plate 5 is slidably sleeved on the vertical rod 4, a pressing plate 6 is fixedly arranged at one side of the transverse plate 5, a through hole 7 is arranged on the inner wall of one side of the fixed box 3, one side of the pressing plate 6 penetrates through the through hole 7 and extends out of the fixed box 3, triangular blocks 8 are fixedly arranged at four corners of the bottom of the pressing plate 6, two opposite triangular blocks 8 are symmetrically arranged, sucking discs 9 are fixedly arranged at four corners of the bottom of the fixed box 3, four L-shaped tubes, four L-shaped tubes 10 are arranged in one-to-one correspondence with four suckers 9, the bottom ends of the L-shaped tubes 10 extend into the corresponding suckers 9, the four L-shaped tubes 10 are arranged in one-to-one correspondence with four triangular blocks 8, pistons 11 are sleeved in the L-shaped tubes 10 in a sealed and sliding manner, transverse rods 12 are fixedly installed on one sides, close to the corresponding triangular blocks 8, of the pistons 11, a plurality of first springs 13 are fixedly installed between the bottom of the transverse plate 5 and the inner wall of the bottom of the fixed box 3, rollers 14 are rotatably installed on one sides, close to the corresponding triangular blocks 8, of the transverse rods 14 and the inclined planes of the corresponding triangular blocks 8 are in rolling contact, rectangular holes 15 are formed in the tops of the transverse rods 12, the same fixed rods 16 are fixedly installed between the inner walls on two sides of the rectangular holes 15, four supporting rods 17 are fixedly installed on the inner wall of the bottom of the, the bracing piece 17 slip cap is established on corresponding dead lever 16, and one side that piston 11 was kept away from corresponding to bracing piece 17 and rectangular hole 15 keep away from between one side inner wall of piston 11 fixed mounting have a plurality of second springs 18, the utility model discloses simple structure, convenient operation, be convenient for fix kit 1 on the workstation fast firm, effectual avoiding is bumped because of the mistake and is caused the measuring liquid in reagent pipe 2 to spill on the workstation, satisfies the use needs, is favorable to the use.

In the utility model, the top of the transverse plate 5 is provided with a perforation 19, the side wall of the perforation 19 is connected with the outside of the vertical rod 4 in a sliding way, the piston 11 is fixedly sleeved with a sealing ring 20, the outside of the sealing ring 20 is connected with the side wall of the corresponding L-shaped pipe 10 in a sliding way, the two sides of the pressing plate 6 are respectively connected with the two side inner walls of the through hole 7 in a sliding way, the number of the second springs 18 in the same rectangular hole 15 is two to four, and the two to four second springs 18 in the same rectangular hole 15 are arranged on the inner wall of one side of the rectangular hole 15 far away from the corresponding piston 11 in an equal interval way, the number of the first springs 13 is two to four, and the two to four first springs 13 are arranged on the inner wall of the bottom of the fixed box 3 in an equal interval way, the utility model has simple structure and convenient operation, is convenient for fixing the reagent box 1 on the workbench quickly and stably, thereby effectively avoiding that the detection liquid, meets the use requirement and is beneficial to use.

The working principle is as follows: when the device is used, the pressing plate 6 is pressed downwards, the pressing plate 6 drives the transverse plate 5 to move downwards on the vertical rod 4, the transverse plate 5 drives the four triangular plates 8 to move downwards, the transverse plate 5 compresses the first springs 13 in the moving process, the triangular plates 8 move downwards and extrude the corresponding rollers 14, the rollers 14 roll on the inclined surfaces of the corresponding triangular plates 8, the rollers 14 move towards the direction close to the corresponding pistons 11 under the action of extrusion force, the rollers 14 drive the corresponding cross rods 12 to move, the cross rods 12 drive the corresponding fixing rods 16 to move, the cross rods 12 compress the corresponding second springs 18 in the moving process, the cross rods 12 drive the corresponding pistons 11 to move, and as the pistons 11 are in sealed sliding installation with the L-shaped tubes 10 through the corresponding sealing rings 20, a gas is pushed out while the pistons 11 move, and the gas is discharged into the suckers 9 through the L-shaped tubes 10, then discharged through the suckers 9, at the moment, the fixed box 3 is placed on the workbench, the fixed box 3 is pressed, so that the four suckers 9 extrude the workbench and deform, the deformed suckers 9 gradually reset to generate a negative pressure inside and are preliminarily adsorbed on the workbench, the pressing plate 6 is slowly loosened, at the moment, the first spring 13 in a compressed state resets, the elastic force of the first spring 13 drives the transverse plate 5 to move upwards and reset on the vertical rod 4, the transverse plate 5 drives the four triangular plates 8 to move upwards and reset, the triangular plates 8 relax the extrusion force on the corresponding idler wheels 14, at the moment, the second spring 18 in a compressed state resets, the elastic force of the second spring 18 drives the corresponding transverse rod 12 to move in a direction away from the piston 11, the transverse rod 12 drives the corresponding fixed rod 16 to move, the transverse rod 12 drives the corresponding idler wheel 14 to move, the transverse rod 12 drives the corresponding piston 11 to move in the L-shaped pipe, the piston 11 is used for pumping air into the sucker 9 through the corresponding L-shaped pipe 10, so that the negative pressure inside the sucker 9 is enhanced, the reagent kit 1 is firmly adsorbed on the workbench, and the pressing plate 6 is conveniently pressed downwards to discharge air to remove adsorption.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. An immunofluorescence staining kit comprises a kit body (1) and a plurality of reagent tubes (2) arranged in the kit body (1), wherein detection liquid is arranged in the reagent tubes (2), the top ends of the reagent tubes (2) extend to the upper side of the kit body (1), and the immunofluorescence staining kit is characterized in that the bottom of the kit body (1) is fixedly provided with a fixed box (3), the inner wall of the bottom of the fixed box (3) is fixedly provided with a vertical rod (4), the vertical rod (4) is sleeved with a transverse plate (5) in a sliding manner, one side of the transverse plate (5) is fixedly provided with a pressing plate (6), the inner wall of one side of the fixed box (3) is provided with a through hole (7), one side of the pressing plate (6) penetrates through the through hole (7) and extends out of the fixed box (3), and the four corners of the bottom of the pressing plate (6) are fixedly provided with, two opposite triangular blocks (8) are symmetrically arranged, sucking discs (9) are fixedly arranged at the four corners of the bottom of the fixed box (3), four L-shaped tubes (10) are arranged in the fixed box (3) symmetrically, the four L-shaped tubes (10) are arranged in a one-to-one correspondence manner with the four sucking discs (9), the bottom ends of the L-shaped tubes (10) extend into the corresponding sucking discs (9), the four L-shaped tubes (10) are arranged in a one-to-one correspondence manner with the four triangular blocks (8), a piston (11) is sleeved in the L-shaped tubes (10) in a sliding manner in a sealing manner, a cross rod (12) is fixedly arranged at one side of the piston (11) close to the corresponding triangular blocks (8), a plurality of first springs (13) are fixedly arranged between the bottom of the cross plate (5) and the inner wall of the bottom of the fixed box (3), and rollers (14) are rotatably arranged at one side of the cross rod (12), gyro wheel (14) and the inclined plane rolling contact of the triangle-shaped piece (8) that corresponds, rectangular hole (15) have been seted up at horizontal pole (12) top, and fixed mounting has same dead lever (16) between the both sides inner wall of rectangular hole (15), fixed mounting has four bracing pieces (17) on the bottom inner wall of fixed case (3), and four bracing pieces (17) and four horizontal poles (12) one-to-one set up, and the top of bracing piece (17) extends to corresponding rectangular hole (15), and bracing piece (17) slip cap is established on corresponding dead lever (16), and fixed mounting has a plurality of second springs (18) between one side inner wall that piston (11) were kept away from to one side of corresponding piston (11) and rectangular hole (15) were kept away from to bracing piece (17).

2. The immunofluorescent staining kit according to claim 1, wherein the top of the transverse plate (5) is provided with a perforation (19), and the side wall of the perforation (19) is slidably connected with the outer side of the vertical rod (4).

3. The immunofluorescent staining kit according to claim 1, wherein the piston (11) is fixedly sleeved with a sealing ring (20), and the outer side of the sealing ring (20) is slidably connected with the side wall of the corresponding L-shaped tube (10).

4. The immunofluorescent staining kit according to claim 1, wherein both sides of the pressing plate (6) are respectively connected with the inner walls of both sides of the through hole (7) in a sliding manner.

5. The immunofluorescent staining kit according to claim 1, wherein the number of the second springs (18) located in the same rectangular hole (15) is two to four, and the two to four second springs (18) located in the same rectangular hole (15) are equally spaced on the inner wall of the rectangular hole (15) on the side away from the corresponding piston (11).

6. The immunofluorescent staining kit according to claim 1, wherein the number of the first springs (13) is two to four, and the two to four first springs (13) are disposed on the bottom inner wall of the fixed box (3) at equal intervals.