CN210215371U - Rhizobium enrichment culture device - Google Patents
Rhizobium enrichment culture device Download PDFInfo
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- CN210215371U CN210215371U CN201920977596.9U CN201920977596U CN210215371U CN 210215371 U CN210215371 U CN 210215371U CN 201920977596 U CN201920977596 U CN 201920977596U CN 210215371 U CN210215371 U CN 210215371U
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- enrichment
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- support frame
- culture
- rhizobium
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Abstract
The utility model provides a rhizobium enrichment culture device, which comprises a support frame, wherein the support frame is sequentially provided with a spraying assembly, a culture assembly and an enrichment assembly from top to bottom; the spraying component comprises a spraying head, the spraying head is fixed with the ball screw mechanism, and the ball screw mechanism drives the spraying head to move left and right; the spraying head is connected with the water outlet of the pump body, and the water inlet of the pump body is connected with the enrichment liquid storage tank; the culture assembly comprises a culture box, wherein a planting plate and a filter layer are sequentially arranged in the culture box from top to bottom, and the culture box is clamped on the first sliding plate; the enrichment assembly comprises an enrichment box, the enrichment box is sleeved on the collection box, and the collection box is clamped on the second sliding plate; the motor and the pump body are in signal connection with the control computer; the utility model provides a rhizobium enrichment culture apparatus can realize regularly, even spray the enrichment liquid to the leguminous plant, has improved rhizobium collection speed.
Description
Technical Field
The utility model belongs to the technical field of the microbial cultivation utensil, concretely relates to rhizobium enrichment culture apparatus.
Background
The soil belongs to a natural environment system, the quantity of rhizobia in a pure soil sample is small, and in addition, the loss in the microbial separation operation process is large, so that the separation of microorganisms with low content is easy to cause, and a certain enrichment culture step is required before the rhizobia in the soil is screened;
enrichment culture, also known as enrichment culture and enrichment culture, means that different characteristics of life activities of different microorganisms are utilized to artificially provide certain specific environmental conditions, so that specific types of microorganisms grow vigorously and are dominant in quantity, the specific microorganisms are more favorable for being separated, and pure culture is conducted.
SUMMERY OF THE UTILITY MODEL
The utility model aims at overcoming the problem that exists among the above-mentioned prior art, provide one kind can be regularly, even spray the enrichment liquid to the leguminous plant on, improved rhizobium collection speed, do not need the artifical rhizobium enrichment culture apparatus that adds the enrichment liquid at any time.
The technical scheme of the utility model is that: a rhizobium enrichment culture device comprises a support frame, wherein the support frame is sequentially provided with a spraying assembly for spraying rhizobium enrichment liquid, a culture assembly for culturing rhizobium and an enrichment assembly for enriching rhizobium from top to bottom; the spraying assembly comprises a spraying head, the spraying head is fixed on the lower surface of a plate body, the plate body is fixedly connected with a ball nut seat, the ball nut seat is fixedly connected with a ball nut, the ball nut is matched with a ball screw, the ball screw is connected with an output shaft of a motor, the spraying head is connected with a water outlet of a pump body, and a water inlet of the pump body is connected with a concentrated solution storage tank; the culture assembly comprises a culture box, wherein a field planting plate and a filter layer are sequentially arranged in the culture box from top to bottom, the culture box is clamped on a first sliding plate, and the left side and the right side of the first sliding plate are in sliding connection with a first sliding groove formed in the side wall of the support frame; the enrichment assembly comprises an enrichment box, the enrichment box is sleeved on the collection box, the collection box is clamped on the second sliding plate, and the left side and the right side of the second sliding plate are in sliding connection with a second sliding groove formed in the side wall of the support frame; the motor and the pump body are in signal connection with the control computer.
The filtering layer is sequentially provided with a first particle layer, a second particle layer and a filter screen from bottom to bottom.
The second particle layer is an activated carbon particle layer, the first particle layer is a quartz sand particle layer, and the filter screen is arranged at an opening at the bottom of the culture box.
Openings are arranged at the left side and the right side of the bottom of the enrichment box, and filter membranes are attached to the openings.
The bottom of the first sliding plate is fixed with a rack which is meshed with a gear, the gear is connected with one end of a rotating shaft, and the other end of the rotating shaft penetrates through the supporting frame and is fixed with a rotating handle.
Both ends all are connected with the support frame through first bearing about above-mentioned ball, and the output shaft of support frame and motor is passed on ball's left side, and the motor is fixed on the support frame.
The spraying head is connected with the water outlet of the pump body through a connecting hose, and the water inlet of the pump body is connected with the enrichment liquid storage tank through a connecting pipe.
The motor, the pump body and the control computer are all electrically connected with an external power supply.
The utility model has the advantages that: the utility model provides a pair of rhizobium enrichment culture apparatus:
1. the enrichment device is provided with the spraying assembly, and the ball screw mechanism is adopted to drive the spraying head to move left and right, so that the enrichment solution can be uniformly sprayed on the original soil and leguminous plants placed on the planting plate, all parts of soil can be ensured to be uniform after receiving the enrichment solution, and rhizobium is enriched and cultured under the condition of protecting the natural environment of the soil to the maximum extent.
2. The motor and the pump body are also in signal connection with the control computer, the control computer is used for controlling the motor and the pump body to be started at regular time, the motor and the pump body are started at regular time, the enrichment liquid is sprayed regularly, the enrichment liquid slowly runs off, the culture speed of rhizobium can be influenced if the enrichment liquid is not supplemented in time, the control computer is adopted for control, the enrichment liquid can be automatically sprayed without the need of people to look after the enrichment liquid at any time and any place, and time and labor are saved.
3. The cultivation subassembly card that will be used for cultivateing the rhizobium is on first sliding plate, and it slides along first spout to drive first sliding plate with rack and gear complex structure, can be convenient when changing the vegetation as required take out the cultivation box, dismantle the washing.
4. The enrichment box is sleeved on the collection box, the collection box is clamped on the second sliding plate, when the enrichment liquid is to be fully collected, the second sliding plate is pulled to take the enrichment liquid down from the supporting frame, and meanwhile, the enrichment liquid filtered by the enrichment box in the collection box is recycled again.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a partially enlarged structural schematic diagram of the rack and pinion assembly of the present invention.
Description of reference numerals:
1. a support frame; 2. a sprinkler head; 3. a plate body; 4. a ball nut seat; 5. a ball nut; 6. a ball screw; 7. a motor; 8. a pump body; 9. a storage tank for the enrichment liquid; 10. a culture box; 11. a field planting plate; 12. a first sliding plate; 13. a first chute; 14. an enrichment cassette; 15. a collection box; 16. a second sliding plate; 17. a second chute; 18. a first layer of particles; 19. a layer of second particles; 20. filtering with a screen; 21. filtering the membrane; 22. a rack; 23. a gear; 24. a rotating shaft; 25. rotating the handle; 26. a connecting hose; 27. and (4) connecting the pipes.
Detailed Description
In the following, an embodiment of the present invention will be described in detail with reference to the drawings, but it should be understood that the scope of the present invention is not limited by the embodiment.
Referring to fig. 1 and fig. 2, an embodiment of the present invention provides a rhizobium enrichment culture apparatus, including a support frame 1, the support frame 1 is provided with a spraying component for spraying rhizobium enrichment liquid, a culture component for culturing rhizobium, and an enrichment component for enriching rhizobium in sequence from top to bottom; the spraying assembly comprises a spraying head 2, the spraying head 2 is fixed on the lower surface of a plate body 3, the plate body 3 is fixedly connected with a ball nut seat 4, the ball nut seat 4 is fixedly connected with a ball nut 5, the ball nut 5 is matched with a ball screw 6, the ball screw 6 is connected with an output shaft of a motor 7, the spraying head 2 is connected with a water outlet of a pump body 8, and a water inlet of the pump body 8 is connected with a concentrated solution storage tank 9; the culture assembly comprises a culture box 10, wherein a field planting plate 11 and a filter layer are sequentially arranged in the culture box 10 from top to bottom, the culture box 10 is clamped on a first sliding plate 12, and the left side and the right side of the first sliding plate 12 are in sliding connection with a first sliding groove 13 formed in the side wall of the support frame 1; the enrichment assembly comprises an enrichment box 14, the enrichment box 14 is sleeved on a collection box 15, the collection box 15 is clamped on a second sliding plate 16, and the left side and the right side of the second sliding plate 16 are in sliding connection with a second sliding groove 17 formed in the side wall of the support frame 1; the motor 7 and the pump body 8 are in signal connection with a control computer.
Further, the filtering layers are a first particle layer 18, a second particle layer 19 and a filter screen 20 from bottom to bottom in sequence, and the filtering layers adopt three-level filtering to block soil particles step by step.
Further, the second particle layer 19 is an activated carbon particle layer, the first particle layer 18 is a quartz sand particle layer, and the filter screen 20 is arranged at an opening at the bottom of the culture box 10, so that rhizobia with small volume can enter the enrichment box along with the nutrient solution.
Furthermore, openings are formed in the left side and the right side of the bottom of the enrichment box 14, filter membranes 21 are attached to the openings, and the filter membranes 21 are used for preventing rhizobia from passing through.
Further, a rack 22 is fixed at the bottom of the first sliding plate 12, the rack 22 is meshed with a gear 23, the gear 23 is connected with one end of a rotating shaft 24, the other end of the rotating shaft 24 penetrates through the support frame 1 and is fixed with a rotating handle 25, and the culture box is conveniently taken out by adopting a structure that the rack 22 is matched with the gear 23.
Furthermore, both ends all are connected with support frame 1 through first bearing about ball 6, and ball 6's left side is passed support frame 1 and is connected with the output shaft of motor 7, and motor 7 is fixed on support frame 1.
Further, the sprinkler head 2 is connected with the water outlet of the pump body 8 through a connecting hose 26, the water inlet of the pump body 8 is connected with the enrichment liquid storage tank 9 through a connecting pipe 27, and the sprinkler head 2 is arranged to evenly spray the enrichment liquid onto the leguminous plants.
Further, the motor 7, the pump body 8 and the control computer are all electrically connected with an external power supply.
The utility model discloses a theory of operation: when the rhizobium enrichment culture device is used, leguminous plants are placed in a culture box, namely the leguminous plants taken from the nature and soil near the root systems of the leguminous plants are moved into the culture box 10 together, the leguminous plants are fixed on a planting plate, then a spraying assembly is started to spray enrichment liquid to the leguminous plants, a motor is started and drives a ball screw to rotate, the ball screw drives a ball nut to advance, the ball nut drives a ball nut seat and a plate body and a spraying head fixed on the lower surface of the plate body to advance along the ball screw, so that the reciprocating motion of the spraying head from left to right from the culture box 10 can be realized, then a pump body is started, the enrichment liquid in the enrichment liquid storage tank is pumped out by the pump body, the pumped enrichment liquid is output to the spraying head by a connecting hose, and the enrichment liquid is sprayed to the leg;
the enrichment liquid sequentially passes through the culture box, the enrichment box and the collection box, soil particles are gradually blocked by the first particle layer, the second particle layer and the filter screen and cannot enter the enrichment box, rhizobia can enter the enrichment box along with nutrient solution due to small cell volume, openings are formed in the left side and the right side of the enrichment box, the aperture of the filter membrane is 0.22-0.45 mu m and used for blocking rhizobia from passing through, the rhizobia with large volume in the enrichment box cannot flow out of the enrichment box, and therefore the rhizobia is reserved in the enrichment box, and meanwhile nutrient substances can flow through the filter membrane and enter the collection box, and the nutrient solution can be secondarily utilized;
after the liquid in the enrichment box is fully collected, the second sliding plate is pulled and slides along a second sliding groove formed in the side wall of the supporting frame, so that the enrichment box can be taken out;
when need changing the leguminous plant sample, when needing to take out the culture box, rotate twist grip, twist grip drives the pivot and rotates, and the pivot drives gear revolve, and the gear drives the rack and gos forward, and the rack drives first sliding plate along first sliding plate roll-off, just can take out the culture box.
To sum up, the utility model provides a pair of rhizobium enrichment culture apparatus can be regularly, even spray the enrichment liquid to leguminous plant, has improved rhizobium collection speed, does not need the manual work to add enrichment liquid, labour saving and time saving at any time.
The above disclosure is only for a few specific embodiments of the present invention, however, the present invention is not limited to the embodiments, and any changes that can be considered by those skilled in the art shall fall within the protection scope of the present invention.
Claims (8)
1. A rhizobium enrichment culture device is characterized by comprising a support frame (1), wherein the support frame (1) is sequentially provided with a spraying component for spraying rhizobium enrichment liquid, a culture component for culturing rhizobium and an enrichment component for enriching rhizobium from top to bottom; the spraying assembly comprises a spraying head (2), the spraying head (2) is fixed on the lower surface of a plate body (3), the plate body (3) is fixedly connected with a ball nut seat (4), the ball nut seat (4) is fixedly connected with a ball nut (5), the ball nut (5) is matched with a ball screw (6), the ball screw (6) is connected with an output shaft of a motor (7), the spraying head (2) is connected with a water outlet of a pump body (8), and a water inlet of the pump body (8) is connected with a concentrated liquid storage tank (9); the cultivation assembly comprises a cultivation box (10), wherein a planting plate (11) and a filter layer are sequentially arranged in the cultivation box (10) from top to bottom, the cultivation box (10) is clamped on a first sliding plate (12), and the left side and the right side of the first sliding plate (12) are in sliding connection with a first sliding groove (13) formed in the side wall of the support frame (1); the enrichment assembly comprises an enrichment box (14), the enrichment box (14) is sleeved on the collection box (15), the collection box (15) is clamped on the second sliding plate (16), and the left side and the right side of the second sliding plate (16) are in sliding connection with a second sliding groove (17) formed in the side wall of the support frame (1); the motor (7) and the pump body (8) are in signal connection with the control computer.
2. A rhizobial enrichment culture device according to claim 1, wherein the filter layer comprises, in order from bottom to bottom, a first granular layer (18), a second granular layer (19) and a screen (20).
3. A rhizobial bacteria enrichment culture device according to claim 2, wherein the second granular layer (19) is an activated carbon granular layer, the first granular layer (18) is a quartz sand granular layer, and the strainer (20) is provided at the bottom opening of the culture box (10).
4. A rhizobial bacteria enrichment culture device according to claim 1, wherein openings are provided on the left and right sides of the bottom of the enrichment cassette (14), and filter membranes (21) are attached to the openings.
5. A rhizobial bacteria culture apparatus as claimed in claim 1, wherein the first sliding plate (12) has a rack (22) fixed to the bottom thereof, the rack (22) is engaged with a gear (23), the gear (23) is connected to one end of a shaft (24), and a rotary handle (25) is fixed to the other end of the shaft (24) through the support frame (1).
6. A rhizobial bacteria enrichment culture device according to claim 1, characterized in that the left and right ends of the ball screw (6) are connected to the support frame (1) through first bearings, the left side of the ball screw (6) passes through the support frame (1) and is connected to the output shaft of the motor (7), and the motor (7) is fixed to the support frame (1).
7. A rhizobial enrichment culture device according to claim 1, wherein the spray head (2) is connected to the outlet of the pump body (8) by a connection hose (26), and the inlet of the pump body (8) is connected to the enrichment solution storage tank (9) by a connection pipe (27).
8. A rhizobial enrichment culture device according to claim 1, wherein the motor (7), pump body (8) and control computer are electrically connected to an external power source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920977596.9U CN210215371U (en) | 2019-06-26 | 2019-06-26 | Rhizobium enrichment culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920977596.9U CN210215371U (en) | 2019-06-26 | 2019-06-26 | Rhizobium enrichment culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210215371U true CN210215371U (en) | 2020-03-31 |
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ID=69934187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920977596.9U Expired - Fee Related CN210215371U (en) | 2019-06-26 | 2019-06-26 | Rhizobium enrichment culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210215371U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350509A (en) * | 2022-03-17 | 2022-04-15 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment biological enrichment and spraying type solid separation culture device |
-
2019
- 2019-06-26 CN CN201920977596.9U patent/CN210215371U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350509A (en) * | 2022-03-17 | 2022-04-15 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment biological enrichment and spraying type solid separation culture device |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200331 Termination date: 20200626 |
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| CF01 | Termination of patent right due to non-payment of annual fee |