CN210184331U

CN210184331U - Cell preservation device

Info

Publication number: CN210184331U
Application number: CN201921014498.1U
Authority: CN
Inventors: Fangcheng Bi; 毕方成
Original assignee: Pacific Kangtai Scientific Instrument (ji'nan) Co Ltd
Current assignee: Pacific Kangtai Scientific Instrument (ji'nan) Co Ltd
Priority date: 2019-06-30
Filing date: 2019-06-30
Publication date: 2020-03-27
Anticipated expiration: 2029-06-30

Abstract

The utility model relates to a cell preservation device, including the body of rod, cell slide glass and sleeve pipe. An intermediate rod for connecting the rod body and the cell slide is arranged between the rod body and the cell slide. The cannula is capable of receiving a portion of the cell slide and the intermediate shaft within its lumen. The tube cavity port of the sleeve can be tightly plugged at the root part of one end of the middle rod close to the rod body. The cross section of the cell slide is rectangular and a plurality of rows of through holes which are arranged alternately are distributed on the end surface. Which can improve the air permeability when the cell sample is attached on the cell slide.

Description

Cell preservation device

Technical Field

The utility model relates to a cell freezing technical field particularly is a cell preservation device.

Background

The continuous development of biotechnology has led to the widespread application of methods for performing vitrification cryopreservation on biological cells.

The prior commonly used vitrification freezing storage device mainly comprises a core bar, a cell slide fixedly arranged at one end of the core bar, a sleeve which can be sleeved outside the core bar to seal the cell slide, and a cotton plug body and a vertical part which are arranged at one end of the sleeve corresponding to the cell slide, wherein the vertical part is relatively positioned at the outer side of the cotton plug body. The cell slide is made of transparent materials and can bear ultralow temperature. In use, biological cells are attached to a section of the cell slide adjacent the free end thereof. In the process of using the existing biological cell preservation rod, it is found that only one side of the biological cell is close to the liquid level when the biological cell is attached to the cell slide, so that the air permeability of the biological cell is relatively poor. In addition, in the process of using the existing biological cell preservation rod, it is found that moving the cell slide into and out of the sleeve is a very delicate operation, the rod body or the sample has a large possibility of touching the inner wall of the sleeve, the sample is possibly damaged, and finally, the survival rate of the preserved cells is remarkably reduced relative to the expected survival rate.

SUMMERY OF THE UTILITY MODEL

In order to make the cell sample have relatively better gas permeability when attaching on the cell slide, the utility model provides a cell preservation device.

The utility model provides a technical scheme that its technical problem adopted is: a cell preservation device comprises a rod body, a cell slide and a sleeve. An intermediate rod for connecting the rod body and the cell slide is arranged between the rod body and the cell slide. The sleeve is capable of receiving portions of the cell slide and the intermediate rod within its lumen with some axial spacing between the end of the cell slide and the tampon body. The tube cavity port of the sleeve can be tightly plugged at the root part of one end of the middle rod close to the rod body. The cross section of the cell slide is rectangular and a plurality of rows of through holes which are arranged alternately are distributed on the end surface.

In a specific embodiment of this patent, the free end of the cell slide is provided with black markings.

In the specific embodiment of this patent, the front side and the back side of the cell slide are respectively provided with a side plate extending along the length direction thereof, so that the cross section of the cell slide is in an "i" shape or a "[" shape. The thickness of the side plate is larger than that of the cell slide, and the function of the side plate is to increase the supporting rigidity of the cell slide.

In a specific embodiment of this patent, the intermediate rod is a tapered rod and the small diameter end of the tapered rod corresponds to the cell slide. The root of the conical rod close to the rod body is provided with a cylindrical section, and the end opening of the tube cavity of the sleeve is matched with the cylindrical section. When the sleeve is sleeved outside the cell slide and the conical rod, the inner wall of the port of the tube cavity is contacted with the outer wall of the cylindrical section, so that the cell slide and the conical rod are tightly connected.

In the specific embodiment of the present patent, a semicircular ring body is formed at one side of the open end of the sleeve, a strip-shaped notch is formed on the axial section of the semicircular ring body, and the axial extension length of the strip-shaped notch is greater than the length of the cell slide, preferably 1.2 to 1.8 times, and particularly 1.5 times of the length of the cell slide. And a supporting block is formed on the side wall of one end of the middle rod, which is close to the cell slide, and when the supporting block is contacted with the inner wall of the semi-circular ring body part, the cell slide is positioned at a position which is flush with the port of the strip-shaped notch or is inwards close to the port relatively. Preferably, the supporting block is in a semicircular shape, the outer diameter of the supporting block is consistent with the inner diameter of the semicircular body part, namely, the inner diameter of the lumen, so that the semicircular surface of the supporting block can be completely contacted with the inner wall of the semicircular body, when the opening end of the cell slide relative to the sleeve moves along the axial direction, the cell slide can be prevented from shaking relative to the sleeve in operation, the cell slide can be effectively prevented from being contacted with the inner wall of the sleeve, the phenomenon that a sample is damaged is avoided finally, and the good survival rate can be realized. The intermediate rod is a cylindrical rod and has an outer diameter smaller than the inner diameter of the lumen, preferably 1/2 to 2/3 of the inner diameter of the lumen. And a cylindrical surface section is formed at the joint position of the middle rod and the rod body, and the outer diameter of the cylindrical surface section is consistent with the inner diameter of the port of the tube cavity, so that the cylindrical surface section can be plugged into the port of the tube cavity at the root part of the strip-shaped notch.

The utility model has the advantages that: which can improve the air permeability when the cell sample is attached on the cell slide. Simultaneously through the change to the body of rod and the sleeve pipe structure that matches, it can also reduce the degree of difficulty in the operation of the body of rod and sleeve pipe looks grafting, reduces the risk that the cell sample received the harm in the operation, can guarantee that the survival rate of being preserved the cell reaches the expectation requirement.

Drawings

Fig. 1 is a schematic diagram of an embodiment of the present patent.

Fig. 2 is a schematic view of a partial structural state of another embodiment of the present disclosure.

Fig. 3 is a schematic view of a partial structural state two of another embodiment of the present disclosure.

FIG. 4 is a schematic cross-sectional view of the embodiment shown in FIG. 2, with a support block disposed at one end of the intermediate rod and disposed at the end of the sleeve.

Fig. 5 is a schematic end view of the port face of the lower casing of the solution shown in fig. 2.

In the figure: 1 rod body, 11 middle rods, 111 supporting blocks, 112 cylindrical surface sections, 12 cell slides, 2 sleeves, 21 tube cavities, 211 strip-shaped notches, 22 tampon bodies and 23 vertical parts

Detailed Description

The drawings in the specification show the structure, ratio, size, etc. only for the purpose of matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and not for the purpose of limiting the present invention, so the present invention does not have the essential meaning in the art, and any structure modification, ratio relationship change or size adjustment should still fall within the scope covered by the technical content disclosed in the present invention without affecting the function and achievable purpose of the present invention. Meanwhile, the terms "upper", "lower", "front", "rear", "middle", and the like used in the present specification are for the sake of clarity only, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof are also considered to be the scope of the present invention without substantial changes in the technical content.

A cell preservation device as shown in FIG. 1 comprises a rod body 1, a cell slide 12 and a sleeve 2. An intermediate rod 11 for connecting the rod body 1 and the cell slide 12 is arranged between the two. The sleeve 2 is able to accommodate the cell slide 12 and part of the intermediate rod 11 within its lumen 21 with a certain axial spacing between the end of the cell slide 12 and the tampon body 22. The end of the lumen 21 of the sleeve 2 can be tightly plugged at the root of one end of the intermediate rod 11 close to the rod body 1. The cross section of the cell slide 12 is rectangular and a plurality of rows of through holes arranged alternately are distributed on the end surface. The cell sample is in the through hole when attached to the cell slide, so that the air permeability of the cell sample can be improved.

The free end of the cell slide 12 is provided with a black mark. This can facilitate observation under a microscope.

The front side and the rear side of the cell slide are respectively provided with a side plate extending along the length direction of the cell slide, so that the cross section of the cell slide is in an I shape or a [ -shape. The thickness of the side plate is larger than that of the cell slide, and the function of the side plate is to increase the supporting rigidity of the cell slide.

As shown in fig. 1, the intermediate rod 11 is a tapered rod, and the small diameter end of the tapered rod corresponds to the cell slide 12. The root of the tapered rod close to the rod body 1 is provided with a cylindrical section, and the port 21 of the tube cavity of the sleeve 2 is matched with the cylindrical section. When the sleeve 2 is sleeved outside the cell slide 12 and the conical rod, the inner wall of the port of the tube cavity 21 is contacted with the outer wall of the cylindrical section, so that the two are tightly connected.

As shown in fig. 2 to 5, the intermediate rod 11 is a cylindrical rod and has an outer diameter smaller than the inner diameter of the lumen 21, preferably 1/2 to 2/3 of the inner diameter of the lumen 21. A cylindrical surface section 112 is formed at the position where the intermediate rod 11 is connected with the rod body 1, and the outer diameter of the cylindrical surface section 112 is consistent with the inner diameter of the port of the tube cavity 21, so that the cylindrical surface section 112 can be plugged into the port of the tube cavity 21.

A semicircular ring body is formed at one side of the opening end of the sleeve 2, a strip-shaped notch 211 is formed on the axial section of the semicircular ring body, and the axial extension length of the strip-shaped notch 211 is greater than the length of the cell slide 12, preferably 1.2 to 1.8 times, and particularly 1.5 times of the length of the cell slide.

A supporting block 111 is formed on the side wall of one end of the middle rod 11 close to the cell slide 12, and when the supporting block 111 contacts with the inner wall of the semi-circular ring body part, the cell slide 12 is in a position flush with the port of the strip-shaped notch 211 or close to the inner side of the port. As shown in fig. 4, preferably, the supporting block 111 is in a semicircular ring shape, and the outer diameter of the supporting block is consistent with the inner diameter of the semicircular ring body part, that is, consistent with the inner diameter of the lumen 21, so that the semicircular surface of the supporting block 111 can be completely contacted with the inner wall of the semicircular ring body, when the cell slide 12 is axially moved relative to the opening end of the casing 2 along the axial direction, the cell slide 12 can be prevented from shaking relative to the casing 2 during operation, and then the cell slide 12 can be effectively prevented from being contacted with the inner wall of the casing 2, which finally helps to prevent the sample from being damaged, and ensures that a good survival rate can be realized.

The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. The present invention can be modified in many ways without departing from the spirit and scope of the present invention, and those skilled in the art can modify or change the embodiments described above without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims

1. The utility model provides a cell preservation device, includes the body of rod, cell slide glass and sleeve pipe, is equipped with the intermediate lever between the body of rod and the cell slide glass, and sheathed tube lumen port can be stopped up and lean on the one end root of the body of rod, its characterized in that at the intermediate lever: the cross section of the cell slide is rectangular and a plurality of rows of through holes which are arranged alternately are distributed on the end surface.

2. The cell preservation apparatus according to claim 1, wherein: the end part of the free end of the cell slide is provided with a black mark.

3. The cell preservation apparatus according to claim 1, wherein: the front side and the rear side of the cell slide are respectively provided with a side plate extending along the length direction of the cell slide, so that the cross section of the cell slide is in an I shape or a [ -shape.

4. A cell preservation device according to any one of claims 1 to 3, wherein: the middle rod is a conical rod, and the small-diameter end of the conical rod corresponds to a cell slide; the root of the conical rod close to the rod body is provided with a cylindrical section, and the end opening of the tube cavity of the sleeve is matched with the cylindrical section.

5. A cell preservation device according to any one of claims 1 to 3, wherein: a semi-circular ring body is formed on one side of the opening end of the sleeve, a strip-shaped notch is formed on the axial section of the semi-circular ring body, and the axial extension length of the strip-shaped notch is greater than the length of the cell slide;

and a supporting block is formed on the side wall of one end of the middle rod, which is close to the cell slide, and when the supporting block is contacted with the inner wall of the semi-circular ring body part, the cell slide is positioned at a position which is flush with the port of the strip-shaped notch or is inwards close to the port relatively.

6. A cell preservation apparatus according to claim 5, wherein: the length of the strip-shaped notch is 1.2 to 1.8 times of the length of the cell slide.

7. A cell preservation apparatus according to claim 5, wherein: the supporting block is in a semi-annular shape, and the outer diameter of the supporting block is consistent with the inner diameter of the semi-annular ring body part.

8. The cell preservation apparatus according to claim 7, wherein: the middle rod is a cylindrical rod, and the outer diameter of the middle rod is smaller than the inner diameter of the tube cavity; and a cylindrical surface section is formed at the joint position of the middle rod and the rod body, and the outer diameter of the cylindrical surface section is consistent with the inner diameter of the port of the tube cavity, so that the cylindrical surface section can be plugged into the port of the tube cavity at the root part of the strip-shaped notch.

9. A cell preservation apparatus according to claim 5, wherein: the middle rod is a cylindrical rod, and the outer diameter of the middle rod is smaller than the inner diameter of the tube cavity; and a cylindrical surface section is formed at the joint position of the middle rod and the rod body, and the outer diameter of the cylindrical surface section is consistent with the inner diameter of the port of the tube cavity, so that the cylindrical surface section can be plugged into the port of the tube cavity at the root part of the strip-shaped notch.