CN210166558U - Microscope with high fluorescence collection rate - Google Patents
Microscope with high fluorescence collection rate Download PDFInfo
- Publication number
- CN210166558U CN210166558U CN201921248596.1U CN201921248596U CN210166558U CN 210166558 U CN210166558 U CN 210166558U CN 201921248596 U CN201921248596 U CN 201921248596U CN 210166558 U CN210166558 U CN 210166558U
- Authority
- CN
- China
- Prior art keywords
- microscope
- objective lens
- fluorescence
- objective
- photoelectric detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000835 fiber Substances 0.000 claims abstract description 10
- 239000010410 layer Substances 0.000 claims description 44
- 239000011241 protective layer Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 4
- 238000012634 optical imaging Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 20
- 230000003287 optical effect Effects 0.000 description 18
- 239000013307 optical fiber Substances 0.000 description 10
- 238000003384 imaging method Methods 0.000 description 6
- 238000012545 processing Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000011810 insulating material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005304 optical glass Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
- Endoscopes (AREA)
Abstract
The utility model relates to an optical imaging field specifically is a high fluorescence collection rate microscope, including the mirror holder, be equipped with the microscope body that is used for placing experimental samples's objective table and is used for detecting experimental samples on the mirror holder, be equipped with the collection optic fibre that is used for exporting laser signal's laser input optic fibre and is used for transmitting fluorescence photon on the microscope body, set up the mounting hole on the microscope body, install the objective body on the mounting hole, the objective body supplies laser signal to pass, be equipped with the photoelectric detector who is used for turning into fluorescence photon the signal of telecommunication on the microscope body, one side that the microscope body was kept away from to the photoelectric detector is close to experimental samples and is used for collecting fluorescence photon. By adopting the scheme, the fluorescence photons which cannot be collected by the microscope objective can be collected, and the fluorescence collection efficiency is improved.
Description
Technical Field
The utility model relates to an optical imaging field specifically is a high fluorescence collection rate microscope.
Background
In nonlinear optical imaging microscopes, in particular multiphoton fluorescence microscopes, near-infrared laser pulses are focused by a microscope objective and excite isotropically emitted electrical signals in a sample. Biological tissue generally exhibits optical properties of strong absorption and high scattering. For epi-illumination fluorescence detection, the same microscope objective is used for both focusing the laser signal and collecting the electrical signal. The intensity of the electrical signal collected by the microscope Objective depends on the numerical Aperture of the microscope Objective and the Objective Front Aperture (OFA). The larger the numerical aperture of the microscope objective and the aperture in front of the objective, the greater the intensity of the electrical signal that the microscope objective can collect. For a microscope objective with a numerical aperture of 0.8 and a magnification of 40X, which is common in two-photon fluorescence microscopes, only less than 10% of the fluorescence in the solid angle of the highly scattered sample is collected by the microscope objective.
Therefore, in recent years, many techniques have been developed for collecting fluorescence photons that cannot be collected by a microscope objective, for example, by arranging 5 to 8 high-na optical fibers around the microscope objective to collect fluorescence that cannot be collected by the microscope objective, 2-fold fluorescence collection efficiency enhancement can be obtained at the high-na microscope objective and 20-fold fluorescence collection efficiency enhancement can be obtained at the low-na microscope objective. For example, a compatible commercial two-photon fluorescence microscope that appeared in 2016, achieved a 2.75 fold increase in fluorescence collection efficiency at a high numerical aperture microscope objective using a total emission detection technique using a quarter ellipsoid mirror.
The above techniques for enhancing fluorescence collection efficiency all employ additional optical elements to collect fluorescence photons that cannot be collected by the microscope objective. Due to the fact that the scattering angle of the fluorescence photons is very large in discreteness, after the fluorescence photons enter the extra collection light path, the multiple reflection path of the fluorescence photons is complex, loss is large, and the actual collection efficiency of the extra optical element is limited. In addition, the shape of the additional optical element is complex, the processing difficulty is high, the cost is high, the size of an instrument using the fluorescence collection efficiency enhancing technology is too large, the imaging area can be shielded, and meanwhile, the electrophysiological experiment operation is hindered.
Therefore, a microscope with high fluorescence collection rate is needed.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a high fluorescence collection rate microscope to solve above-mentioned technical problem.
The utility model provides a basic scheme: the utility model provides a high fluorescence collection rate microscope, includes the mirror holder, be equipped with the objective table that is used for placing experimental samples on the mirror holder and the microscope body that is used for detecting experimental samples, be equipped with the collection optic fibre that is used for exporting laser signal's laser input optic fibre and is used for transmitting fluorescence photon on the microscope body, set up the mounting hole on the microscope body, install the objective body on the mounting hole, the objective body supplies laser signal to pass, be equipped with on the microscope body and be used for turning into the photoelectric detector of signal of telecommunication with fluorescence photon, one side that the photoelectric detector kept away from the microscope body is close to experimental samples and is used for collecting fluorescence photon.
The basic scheme has the following working principle and beneficial effects: the setting of mirror holder, for the objective table, the microscope body provides the support, the setting of objective table, steady support experimental samples, be convenient for observe through the microscope body, the setting of microscope body, observe experimental samples, provide the testing result, the setting of laser input optic fibre, to this internal output laser signal of microscope, and shine to experimental samples from the microscope body on, collect the setting of optic fibre, will be through experimental samples dorsad reflection and backscatter, and the fluorescence photon transmission to external equipment of the acquisition after the filtering, analyze fluorescence photon through external equipment, thereby obtain corresponding experimental image.
The installation hole is used for installing the objective lens body; the arrangement of the objective lens body can enable laser signals to pass through the objective lens body and enter the microscope body to be collected after being reflected and scattered back by the experiment sample. Laser signals pass through the objective lens body and reach an experimental sample, light paths of the laser signals are in different angles due to back reflection and back scattering, a part of the laser signals enter the microscope body through the objective lens body, and after passing through related elements, fluorescence photons in the received laser signals are transmitted to an external computer through the collection optical fibers by the microscope body and are converted into electric signals. The other part of laser signals pass through a photoelectric detector, fluorescence photons in the laser signals are collected by the photoelectric detector and converted into electric signals, the electric signals are transmitted to an external computer through an electric wire, and the external computer integrates the two electric signals to obtain a final detection image.
One side of the photoelectric detector far away from the microscope body is close to the experimental sample, and laser signals reflected back and scattered back through the experimental sample are directly collected without multiple reflections, so that the loss of collected fluorescence photons is reduced. Compared with the traditional electronic scanning microscope (namely, the microscope with the function of converting the fluorescence photons in the laser signals passing through the objective lens body into electric signals), the fluorescence photons which cannot be received by the objective lens body are collected through the photoelectric detector, so that the fluorescence collection efficiency is improved, the signal-to-noise ratio of imaging is improved, and the imaging depth in a high-scattering medium is improved
Further, the photoelectric detector is abutted against the objective lens body. Has the advantages that: the laser signal through experimental sample back reflection and back scattering concentrates on the position relative with the objective body mostly, and the laser signal uses the objective body to outwards reduce gradually as the center, therefore photoelectric detector offsets with the objective body, can collect more fluorescence photons, further improves fluorescence collection efficiency.
Further, one side of the photoelectric detector, which is far away from the microscope body, is parallel to the focal plane of the objective lens body. Has the advantages that: one side that the photoelectric detector kept away from the microscope body is parallel with the focal plane of objective body, represents the photoelectric detector promptly and flushes with the surface of microscope body, and the photoelectric detector is embedded in the microscope body to avoid because the outstanding damage that causes of photoelectric detector.
Further, the distance from one end of the photoelectric detector, which is abutted against the objective lens body, to the focal plane of the objective lens body is greater than the distance from one end of the photoelectric detector, which is far away from the objective lens body, to the focal plane of the objective lens body. Has the advantages that: the photodetector has an angle that is greater than the photodetector level, i.e., the range over which the fluorescence photons are collected.
Further, the photodetector includes a filter layer for filtering out back-reflected and back-scattered laser signals and passing through fluorescence photons. Has the advantages that: the filter layer is arranged to filter laser signals with long wavelength, so that fluorescent photons with short wavelength penetrate through the filter layer, and the fluorescent photons can be conveniently collected subsequently.
Further, the photodetector further includes a photosensitive layer for converting the fluorescence photons passing through the filter layer into an electrical signal. Has the advantages that: the arrangement of the photosensitive layer converts the fluorescent photons penetrating through the filter layer into electric signals, thereby facilitating the subsequent data transmission.
Further, the photoelectric detector also comprises a protective layer used for transmitting light, and the protective layer wraps the filter layer and the photosensitive layer. Has the advantages that: the setting of protective layer keeps apart the experimental sample, avoids experimental sample and light filter contact, keeps apart photosensitive layer and experimental sample, microscope body, objective body simultaneously, avoids causing the injury to experimental sample, microscope body, objective body, operating personnel etc. when required high voltage when the photosensitive layer is components that have internal gain such as avalanche photodiode
Further, the photosensitive layer is connected with a cable for transmitting an electric signal. Has the advantages that: and transmitting the electrical signal converted by the photosensitive layer to an external computer through a cable for processing so as to obtain a corresponding image.
Further, the photosensitive layer comprises a plurality of photosensitive elements, and the photosensitive elements are circumferentially distributed by taking the objective lens body as a center. Has the advantages that: the fluorescence photons are collected by the photosensitive elements which are circumferentially distributed, so that the fluorescence photons which cannot be received by the objective body are collected, and the fluorescence collection efficiency is improved.
Furthermore, the photosensitive layer comprises a plurality of photosensitive elements, a micro-lens array is arranged on one side, away from the microscope body, of the protective layer, and the photosensitive elements correspond to the micro-lens positions of the micro-lens array one by one. Has the advantages that: by arranging the micro-lens array, laser signals are focused on each micro-lens in the micro-lens array, the light sensing efficiency is improved, so that each light sensing element collects more fluorescence photons, and the fluorescence collection efficiency is improved.
Drawings
FIG. 1 is a front cross-sectional view of a high fluorescence collection rate microscope according to a first embodiment of the present invention;
FIG. 2 is a cross-sectional view of a photodetector of a high fluorescence collection rate microscope of the present invention;
fig. 3 is a top cross-sectional view of a photodetector according to a first embodiment of the high fluorescence collection rate microscope of the present invention;
FIG. 4 is a front cross-sectional view of a second embodiment of a high fluorescence collection rate microscope of the present invention;
fig. 5 is a top cross-sectional view of a photodetector according to a second embodiment of the high fluorescence collection rate microscope of the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the microscope body 1, the laser input optical fiber 101, the collection optical fiber 102, the objective lens body 103, the photodetector 2, the protective layer 201, the filter layer 202, the photosensitive layer 203, and the driving circuit 204.
Example one
The utility model provides a high fluorescence collection rate microscope, includes the mirror holder, and screw fixedly connected with base is passed through to the bottom of mirror holder, and it has the bracing piece to bond on the base, and screw fixedly connected with objective table is passed through to the one end that the base was kept away from to the bracing piece, and the top surface of objective table is used for placing experimental sample, and screw fixedly connected with microscope body 1 is passed through at the top of mirror holder, and microscope body 1 is located same one side of mirror holder with the objective table. The shapes and positional relationships among the frame, the stage, and the microscope body 1 are not limited to those described in the present application, and any of the related configurations of the microscope in the related art may be adopted.
The side wall of the microscope body 1 is provided with a first through hole for the laser input optical fiber 101 to pass through, one end of the laser input optical fiber 101 is connected with external laser equipment, and the other end is positioned in the microscope body 1 and used for outputting laser signals. The top of the microscope body 1 is provided with a second through hole for the collection optical fiber 102 to pass through, one end of the collection optical fiber 102 is located in the microscope body 1 and used for collecting fluorescence photons, and the other end of the collection optical fiber is connected with external photoelectric imaging equipment.
As shown in fig. 1, a collimating lens, a reflecting mirror, a dichroic mirror scanner, an objective lens body 103 and a focusing lens are further disposed in the microscope body 1, the objective lens body 103 is an aspheric lens, and the collimating lens is configured to collimate the laser signal output from the laser input fiber 101, reduce chromatic aberration between the laser signals of different frequencies, and output the laser signal to the reflecting mirror. The curvature radius of the objective lens body 103 of the aspherical lens varies with the central axis, so as to improve the optical quality, reduce the number of optical elements, and reduce the design cost.
The dichroic mirror scanner is used for separating a laser signal and a nonlinear optical signal (namely, fluorescence photons) and outputting the nonlinear optical signal, and is also used for changing an incident angle of the laser signal to enable the laser signal to perform two-dimensional scanning on a plane of an internal tissue of an experimental sample, namely, the dichroic scanner outputs the laser signal to the objective lens body 103, the objective lens body 103 is used for converging the laser signal from the dichroic mirror scanner on the experimental sample to excite the experimental sample to generate the nonlinear optical signal, then the objective lens body 103 receives and inputs the nonlinear optical signal to the dichroic scanner, the nonlinear optical signal is transmitted from the dichroic scanner to the focusing lens, and the focusing lens is used for effectively collecting the nonlinear optical signal. The focusing lens feeds back the nonlinear optical signal to an external photoelectric imaging device, and the external photoelectric imaging device consists of a plurality of photomultiplier detectors, a plurality of dichroic mirrors, a plurality of optical filters and a plurality of focusing lenses and is used for receiving the nonlinear optical signal transmitted by the collection optical fiber 102 and completing photoelectric conversion for processing by a computer. The arrangement in the microscope body 1, how the laser signal is output from the laser input fiber 101, how the nonlinear optical signal is converted into an electrical signal, and the like are described in detail in the micro optical probe of publication No. CN108261179A, and therefore, they are not described in detail.
The bottom of the microscope body 1 is provided with a mounting hole, the objective lens body 103 is mounted in the mounting hole, and the objective lens body 103 is used for laser signals to pass through. The bottom of microscope body 1 is embedded to have photodetector 2 of converting fluorescence photon into the signal of telecommunication, photodetector 2's top surface offsets with microscope body 1, photodetector 2's one end offsets with objective body 103, photodetector 2 and objective body 103 offset one end to objective body 103's focal plane's distance is greater than photodetector 2 and keeps away from objective body 103's one end to objective body 103's focal plane's distance (being that photodetector 2 is higher than photodetector 2 and objective body 103 offset one end) promptly, photodetector 2's bottom surface is used for being close to the experimental sample and collects fluorescence photon (being that when photodetector 2 is the ring form, its form looks like loudspeaker, the aperture from top to bottom crescent).
The center of the photoelectric detector 2 is provided with a through hole for the objective lens body 103 to pass through, the hole wall of the through hole is abutted against the edge of the objective lens body 103, and laser signals reflected and scattered back by the experimental sample enter the microscope body 1 through the objective lens body 103. Of course, the center of the photodetector 2 may not be provided with a through hole, but a transparent material capable of transmitting the laser signal is adopted, so that the laser signal reflected and backscattered by the experimental sample sequentially passes through the transparent material and the objective lens body 103 and enters the microscope body 1. In this embodiment, the photodetector 2 having a through hole through which the objective lens body 103 passes is preferably provided at the center, that is, the photodetector 2 has a circular ring-shaped structure.
As shown in fig. 2, the photodetector 2 includes a filter layer 202, a photosensitive layer 203 and a driving circuit 204 sequentially arranged from bottom to top, wherein the filter layer 202 is a filter made of an insulating material capable of transmitting visible light, and the filter is used for filtering a laser signal with a long wavelength and transmitting fluorescence photons with a short wavelength.
The photosensitive layer 203 can be a photosensitive element, preferably a single large area avalanche diode, which is shaped as a circular ring structure and can be obtained by mechanical drilling or etching. As shown in fig. 3, the photosensitive layer 203 may also employ a plurality of photosensitive elements, preferably a plurality of avalanche diodes of conventional size, which are circumferentially distributed around the objective body 103. In the present embodiment, the photosensitive layer 203 preferably employs several photosensitive elements, i.e., several avalanche diodes, and the photosensitive layer 203 is used for collecting fluorescent photons that are not received by the objective lens body 103.
Since the photosensitive layer 203 is an avalanche photodiode with internal gain elements and the driving voltage is as high as several hundred to 2000 volts, the driving circuit 204 is used to provide high voltage to the photosensitive layer 203. The photoelectric detector 2 is further provided with a cable interface, the driving circuit 204 and the photosensitive layer 203 are both electrically connected with the cable interface, the cable interface is connected with a cable, the cable is used for transmitting electric signals, and one end, away from the photosensitive layer 203, of the cable is connected with an external computer and used for transmitting the electric signals to the external computer to be processed by the external computer. The conversion of the fluorescence photons into electrical signals by the avalanche diode and the transmission of the electrical signals to an external computer via a cable for processing are prior art and therefore not described in detail.
The filter layer 202, the photosensitive layer 203 and the driving circuit 204 are covered with a protection layer 201, the protection layer 201 is made of an insulating material that can transmit visible light, and the insulating material is preferably optical glass in this embodiment. The top surface of the protective layer 201 is abutted against the microscope body 1, the bottom surface of the protective layer 201 is close to or in contact with an experimental sample, and the ground of the protective layer 201 is plated with an anti-reflection optical coating for improving the transmittance of fluorescence photons.
Example two
The difference between the present embodiment and the first embodiment is: the shape of the photodetector 2, the arrangement of the photosensitive layer 203, and the arrangement of the bottom surface of the protective layer 201. As shown in fig. 4 and 5, in the present embodiment, the photodetector 2 has a square ring structure, and the bottom surface of the photodetector 2 (i.e. the side of the photodetector 2 away from the microscope body 1) is flush with the outer surface of the microscope body 1 (i.e. the side of the photodetector 2 away from the microscope body 1 is parallel to the focal plane of the objective lens body 103), and the bottom surface of the protection layer 201 is a microlens array having a plurality of rectangular microlenses.
The photosensitive layer 203 may be two-dimensional pixel photosensors, such as CCD (charge coupled device) devices, CMOS (metal semiconductor oxide) devices, FPA (focal plane array) devices, PMT (photomultiplier tube) devices, single photon counting devices, or hybrid devices based on any of the above photoelectric conversion principles, and in this embodiment, the photosensitive layer 203 is preferably Hybrid Photodetectors (HPD) of hamamatsu corporation, each of which corresponds to a position of a microlens one by one.
The above description is only for the embodiments of the present invention, and the common general knowledge of the known specific structures and characteristics in the schemes is not described herein too much, and those skilled in the art will know all the common technical knowledge in the technical field of the present invention before the application date or the priority date, can know all the prior art in this field, and have the ability to apply the conventional experimental means before this date, and those skilled in the art can combine their own ability to perfect and implement the schemes, and some typical known structures or known methods should not become obstacles for those skilled in the art to implement the present application. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. The utility model provides a high fluorescence collection rate microscope, includes the mirror holder, be equipped with the objective table that is used for placing experimental samples on the mirror holder and be used for detecting experimental samples's microscope body, be equipped with the collection optic fibre that is used for exporting laser signal's laser input optic fibre and is used for transmitting fluorescence photon on the microscope body, its characterized in that: the microscope body is provided with a mounting hole, the mounting hole is provided with an objective lens body, the objective lens body is used for laser signals to pass through, the microscope body is provided with a photoelectric detector used for converting fluorescence photons into electric signals, and one side, far away from the microscope body, of the photoelectric detector is close to an experimental sample and used for collecting the fluorescence photons.
2. A high fluorescence collection rate microscope according to claim 1, wherein: the photoelectric detector is abutted against the objective lens body.
3. A high fluorescence collection rate microscope according to claim 2, wherein: one side of the photoelectric detector, which is far away from the microscope body, is parallel to the focal plane of the objective lens body.
4. A high fluorescence collection rate microscope according to claim 2, wherein: the distance from one end of the photoelectric detector, which is abutted against the objective lens body, to the focal plane of the objective lens body is greater than the distance from one end of the photoelectric detector, which is far away from the objective lens body, to the focal plane of the objective lens body.
5. A high fluorescence collection rate microscope according to claim 1, wherein: the photodetector includes a filter layer for filtering out back-reflected and back-scattered laser signals and passing through fluorescence photons.
6. The microscope of claim 5, wherein: the photodetector further includes a photosensitive layer for converting fluorescent photons passing through the filter layer into an electrical signal.
7. The microscope of claim 6, wherein: the photoelectric detector also comprises a protective layer used for transmitting light, and the protective layer wraps the filter layer and the photosensitive layer.
8. A high fluorescence collection rate microscope according to claim 7, wherein: the photosensitive layer is connected with a cable for transmitting electric signals.
9. A high fluorescence collection rate microscope according to any one of claims 6 to 8 wherein: the photosensitive layer comprises a plurality of photosensitive elements, and the photosensitive elements are circumferentially distributed by taking the objective lens body as a center.
10. A high fluorescence collection rate microscope according to claim 7 or 8, wherein: the photosensitive layer comprises a plurality of photosensitive elements, a micro-lens array is arranged on one side, away from the microscope body, of the protective layer, and the photosensitive elements correspond to the micro-lenses of the micro-lens array in position one to one.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2019102107307 | 2019-03-18 | ||
| CN201910210730 | 2019-03-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210166558U true CN210166558U (en) | 2020-03-20 |
Family
ID=69448200
Family Applications (14)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921248016.9U Expired - Fee Related CN210155402U (en) | 2019-03-18 | 2019-08-02 | Microscopic probe containing extended photoelectric detector |
| CN201910712515.7A Pending CN111722389A (en) | 2019-03-18 | 2019-08-02 | Miniature endoscope with high fluorescence collection rate |
| CN201921248510.5U Expired - Fee Related CN210155403U (en) | 2019-03-18 | 2019-08-02 | Scanning device for microscope objective |
| CN201910712514.2A Pending CN111722388A (en) | 2019-03-18 | 2019-08-02 | Three-dimensional miniature endoscope |
| CN201921248596.1U Expired - Fee Related CN210166558U (en) | 2019-03-18 | 2019-08-02 | Microscope with high fluorescence collection rate |
| CN201921248598.0U Expired - Fee Related CN210155405U (en) | 2019-03-18 | 2019-08-02 | Micro objective lens comprising photoelectric detector |
| CN201921247896.8U Expired - Fee Related CN210155400U (en) | 2019-03-18 | 2019-08-02 | Miniature endoscope with high fluorescence collection rate |
| CN201921248381.XU Expired - Fee Related CN210166556U (en) | 2019-03-18 | 2019-08-02 | Three-dimensional miniature endoscope |
| CN201910718520.9A Pending CN111722391A (en) | 2019-03-18 | 2019-08-05 | Three-dimensional head-mounted microscope |
| CN201910717814.XA Pending CN111722390A (en) | 2019-03-18 | 2019-08-05 | Head-mounted microscope with high fluorescence collection rate |
| CN201921256738.9U Expired - Fee Related CN210155406U (en) | 2019-03-18 | 2019-08-05 | Three-dimensional head-mounted microscope |
| CN201921258175.7U Expired - Fee Related CN210572988U (en) | 2019-03-18 | 2019-08-05 | Head-mounted microscope with high fluorescence collection rate |
| CN201911268411.8A Pending CN110794563A (en) | 2019-03-18 | 2019-12-11 | High fluorescence collection rate hand-held type microscope |
| CN201922218716.XU Active CN211086789U (en) | 2019-03-18 | 2019-12-11 | High fluorescence collection rate hand-held type microscope |
Family Applications Before (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921248016.9U Expired - Fee Related CN210155402U (en) | 2019-03-18 | 2019-08-02 | Microscopic probe containing extended photoelectric detector |
| CN201910712515.7A Pending CN111722389A (en) | 2019-03-18 | 2019-08-02 | Miniature endoscope with high fluorescence collection rate |
| CN201921248510.5U Expired - Fee Related CN210155403U (en) | 2019-03-18 | 2019-08-02 | Scanning device for microscope objective |
| CN201910712514.2A Pending CN111722388A (en) | 2019-03-18 | 2019-08-02 | Three-dimensional miniature endoscope |
Family Applications After (9)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921248598.0U Expired - Fee Related CN210155405U (en) | 2019-03-18 | 2019-08-02 | Micro objective lens comprising photoelectric detector |
| CN201921247896.8U Expired - Fee Related CN210155400U (en) | 2019-03-18 | 2019-08-02 | Miniature endoscope with high fluorescence collection rate |
| CN201921248381.XU Expired - Fee Related CN210166556U (en) | 2019-03-18 | 2019-08-02 | Three-dimensional miniature endoscope |
| CN201910718520.9A Pending CN111722391A (en) | 2019-03-18 | 2019-08-05 | Three-dimensional head-mounted microscope |
| CN201910717814.XA Pending CN111722390A (en) | 2019-03-18 | 2019-08-05 | Head-mounted microscope with high fluorescence collection rate |
| CN201921256738.9U Expired - Fee Related CN210155406U (en) | 2019-03-18 | 2019-08-05 | Three-dimensional head-mounted microscope |
| CN201921258175.7U Expired - Fee Related CN210572988U (en) | 2019-03-18 | 2019-08-05 | Head-mounted microscope with high fluorescence collection rate |
| CN201911268411.8A Pending CN110794563A (en) | 2019-03-18 | 2019-12-11 | High fluorescence collection rate hand-held type microscope |
| CN201922218716.XU Active CN211086789U (en) | 2019-03-18 | 2019-12-11 | High fluorescence collection rate hand-held type microscope |
Country Status (1)
| Country | Link |
|---|---|
| CN (14) | CN210155402U (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113552710B (en) * | 2021-09-17 | 2022-01-18 | 清华大学 | Multi-plane microscopic imaging system based on gradient variable refractive index lens |
| CN115128788B (en) * | 2022-05-30 | 2023-11-28 | 中国人民解放军国防科技大学 | Horizontally placed microscopic device parallel to observed object |
-
2019
- 2019-08-02 CN CN201921248016.9U patent/CN210155402U/en not_active Expired - Fee Related
- 2019-08-02 CN CN201910712515.7A patent/CN111722389A/en active Pending
- 2019-08-02 CN CN201921248510.5U patent/CN210155403U/en not_active Expired - Fee Related
- 2019-08-02 CN CN201910712514.2A patent/CN111722388A/en active Pending
- 2019-08-02 CN CN201921248596.1U patent/CN210166558U/en not_active Expired - Fee Related
- 2019-08-02 CN CN201921248598.0U patent/CN210155405U/en not_active Expired - Fee Related
- 2019-08-02 CN CN201921247896.8U patent/CN210155400U/en not_active Expired - Fee Related
- 2019-08-02 CN CN201921248381.XU patent/CN210166556U/en not_active Expired - Fee Related
- 2019-08-05 CN CN201910718520.9A patent/CN111722391A/en active Pending
- 2019-08-05 CN CN201910717814.XA patent/CN111722390A/en active Pending
- 2019-08-05 CN CN201921256738.9U patent/CN210155406U/en not_active Expired - Fee Related
- 2019-08-05 CN CN201921258175.7U patent/CN210572988U/en not_active Expired - Fee Related
- 2019-12-11 CN CN201911268411.8A patent/CN110794563A/en active Pending
- 2019-12-11 CN CN201922218716.XU patent/CN211086789U/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN210155402U (en) | 2020-03-17 |
| CN111722388A (en) | 2020-09-29 |
| CN111722391A (en) | 2020-09-29 |
| CN210155406U (en) | 2020-03-17 |
| CN210155403U (en) | 2020-03-17 |
| CN110794563A (en) | 2020-02-14 |
| CN211086789U (en) | 2020-07-24 |
| CN111722389A (en) | 2020-09-29 |
| CN210155405U (en) | 2020-03-17 |
| CN210572988U (en) | 2020-05-19 |
| CN210155400U (en) | 2020-03-17 |
| CN210166556U (en) | 2020-03-20 |
| CN111722390A (en) | 2020-09-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102879088B (en) | Device and method for detecting light | |
| TWI403756B (en) | 3d optical coherent tomography with confocal imaging apparatus | |
| CN101915752B (en) | Laser scanning imaging device | |
| CN205539686U (en) | Burnt spectromicroscope of small -size scanning copolymerization | |
| KR20140027113A (en) | Light guided pixel | |
| JP6596001B2 (en) | Multifocal multiphoton imaging system and method | |
| CN210166558U (en) | Microscope with high fluorescence collection rate | |
| US20150077843A1 (en) | High-resolution scanning microscopy | |
| EP2771900B1 (en) | Integrated backscattered electron detector with cathodoluminescence collection optics | |
| US9395296B1 (en) | Two-dimensional optical spot location using a one-dimensional detector array | |
| CN102507448B (en) | Detecting system and detecting method for biochemical analyzer | |
| US20200166739A1 (en) | Systems and Methods for Configurable Miniature Microscopy | |
| KR20080052229A (en) | Inserted microlens array in laser radar | |
| CN210155428U (en) | Resonance scanning imaging structure, microscope and microprobe | |
| JP2009019961A (en) | Fluorescence detecting system | |
| US20060060189A1 (en) | Optical reflector and optical collection system | |
| US8393010B2 (en) | Near-field scanning optical microscope | |
| US6987274B1 (en) | Light detection and imaging system and method including an array of sensors | |
| CN109211858B (en) | Optical module of multichannel laser scanning imager | |
| CN217838953U (en) | Hole-by-hole scanning multi-channel optical path system based on real-time fluorescent quantitative PCR | |
| US11976971B2 (en) | Spectrograph recycling | |
| CN211381246U (en) | Multi-point scanning confocal endoscope | |
| CN210605257U (en) | Optical receiving device and optical measuring device | |
| JP2013044885A (en) | Enlargement imaging device and image sensor | |
| CN116087159A (en) | Immunofluorescence analyzer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200320 |