CN210166310U - Thin-layer flow cell for detecting single molecules and single nanoparticles with ultrahigh sensitivity - Google Patents
Thin-layer flow cell for detecting single molecules and single nanoparticles with ultrahigh sensitivity Download PDFInfo
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- CN210166310U CN210166310U CN201920779480.4U CN201920779480U CN210166310U CN 210166310 U CN210166310 U CN 210166310U CN 201920779480 U CN201920779480 U CN 201920779480U CN 210166310 U CN210166310 U CN 210166310U
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Abstract
The utility model discloses a thin layer flow cell of super high sensitivity detection unimolecule and single nanometer particle, a serial communication port, including base, flow cell main part and connecting pipe, flow cell main part sets up in the pedestal top surface, and flow cell main part bottom surface is equipped with the indent structure and constitutes microchannel with the pedestal top surface, and flow cell main part is equipped with the intercommunicating pore and stretches into to flow cell main part inside and lead to with the microchannel from the surface, and the connecting pipe stretches into flow cell main part inside and leads to with the microchannel through the intercommunicating pore, and microchannel height range is 20nm-100 um. Compared with the prior art, the utility model discloses technical scheme has advantages such as simple structure, convenient detection and accuracy height, can make thin layer flow cell can conveniently observe single nanometer particle and single fluorescence particle molecule in SPR microscope and total internal reflection fluorescence microscope, and furthest improves fluorescence molecule and nanometer particle and reaches the sensitivity of superelevation.
Description
Technical Field
The utility model relates to a microscopic imaging technology field of ultra-high sensitivity, in particular to thin layer flow cell of ultra-high sensitivity detection unimolecule and single nanometer particle.
Background
Surface Plasmon Resonance (SPR) is a label-free, real-time, rapid and sensitive detection means, in which SPR technology can be applied to studies of intermolecular interaction and protein conformational structural change, SPR based on evanescent waves should be applied to biochemical reaction systems during the last 80 th century, and the first commercial SPR instrument was born in 1991, which is applicable to testing receptor-ligand interaction, and then SPR technology was fully developed and can be used to screen receptors or ligands on membranes for several decades. In recent years, SPR microscopic imaging technology, which is a combination of SPR technology and microscopic imaging technology, has been applied to imaging technology of single nanoparticles, single cells, and single viruses.
The imaging-based SPR microscope can realize the imaging display of single nano particle, even single molecule and biochemical reaction thereof rapidly and with high sensitivity. Since the SPR microscope image contrast of a single nanoparticle depends on its dielectric constant, i.e. is suitable for any material physical property, the SPR microscope can be used to study various types of nanoparticles, such as metal nanoparticles, semiconductors, metal oxides, organic polymer nanoparticles, and biological nanoparticles (bacteria and viruses, etc.).
The detection accuracy of the SPR microscope is inversely proportional to the third power of the particle diameter, while the dark field fluorescence microscope is inversely proportional to the sixth power of the particle diameter, so that the SPR microscope is more sensitive for detecting small nanoparticles than the dark field fluorescence microscope. More importantly, the SPR microscope allows observation of soft nanomaterials, such as viruses, proteins and other nanoparticles, without labeling, by coupling incident light to the surface of the dielectric layer for excitation to produce surface plasmon polaritons, which resonate at the same time as evanescent wave energy produced by total internal reflection of the incident light, thereby sharply reducing the energy of the reflected light. Therefore, the surface plasma resonance microscope is very sensitive to the refractive index change of the medium layer, and materials with different refractive indexes can be distinguished to form images with different light and shade, so that a surface plasma resonance microscopic imaging image with high resolution and free of fluorescence marks is formed.
In the prior art, the principle of microscopic imaging, whether SPR microscopy or total internal reflection fluorescence microscopy, is related to evanescent waves, which are, however, within a thin layer of about 500nm at the surface of the sample. Samples above 500nm height from the substrate were not observed in both SPR and total internal reflection fluorescence microscopes. And the nano particles or fluorescent molecules generate Brownian motion, the particles with the height of more than 500nm can move into an evanescent field or collide with the surface of the substrate to be detected, but the Brownian motion is irregular, so that the size limitation of the micro-channel is crucial to the influence of the signal intensity.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a simple structure, conveniently detect and the high thin layer flow cell of super high sensitivity detection unimolecule and single nanometer particle of accuracy, aim at making thin layer flow cell can conveniently observe single nanometer particle and single fluorescence particle molecule in SPR microscope and total internal reflection fluorescence microscope, furthest improves fluorescence molecule and nanometer particle and reaches super high sensitivity.
In order to achieve the above object, the utility model provides a thin layer flow cell of super high sensitivity detection unimolecule and single nanometer particle, including base, flow cell main part and connecting pipe, flow cell main part sets up in the pedestal face, flow cell main part bottom surface be equipped with indent structure and constitute the microchannel with the pedestal face, flow cell main part is equipped with the intercommunicating pore and stretches into inside to flow cell main part and lead to with the microchannel from the surface, the connecting pipe stretches into inside to flow cell main part and leads to with the microchannel through the intercommunicating pore.
Preferably, the microchannel height ranges from 20nm to 100 um.
Preferably, the surface of the base is provided with a groove structure, and the bottom of the flow cell main body is at least partially embedded into the groove structure.
Preferably, the bottom edge of the flow cell main body is provided with a downward surrounding edge structure, and the surrounding edge structure is at least partially sleeved on the top of the base.
Preferably, after the base and the flow cell main body are connected up and down, the side parts at two sides are respectively clamped into the groove inner parts of the clamps to clamp the base and the flow cell main body up and down.
Preferably, the bottom end edge of the connecting pipe is flush with the top surface of the micro-channel.
Preferably, the edge of the bottom end of the connecting pipe is flush with the bottom surface of the micro-channel, a notch is formed in the bottom end of the connecting pipe and communicated with the micro-channel, and the height of the notch is larger than or equal to the height of the micro-channel.
Preferably, the connecting pipe comprises a liquid inlet pipe and a liquid outlet pipe, and the liquid inlet pipe and/or the liquid outlet pipe are connected with the pump.
Preferably, the pump is a peristaltic pump.
Preferably, the base is made of any one of common glass, glass with a gold or silver or platinum thin layer plated on the top surface, ITO conductive glass, AZO conductive glass and FTO conductive glass, the flow cell body is made of any one or more of polymethyl methacrylate, polydimethylsiloxane, epoxy resin, polyurethane and polyamide, the curing agent adopted in the manufacturing process of the flow cell body is any one or more of SYLGARD 184 curing agent, ethylenediamine, m-phenylenediamine and imidazole, and the connecting pipe is made of polytetrafluoroethylene.
The utility model discloses technical scheme prior art has following advantage relatively:
the utility model discloses technical scheme is through silicon chip lithography and organic polymer rendition technique preparation flow cell main part, punches and the connecting pipe that links to each other with flow cell main part again, through set up the indent structure and constitute the microchannel with the base top surface in flow cell bottom surface to realize that detection solution can flow in the microchannel. Meanwhile, the connecting pipe is communicated with the micro-channel and then connected with the pump, so that the rapid speed of the detection solution is increased. More importantly, the utility model discloses technical scheme's microchannel height scope is 20nm-100um, and is minimum through the height dimension who adjusts the microchannel, like this in the detection solution of same time and same concentration, the particle outside the evanescent field is because brownian motion runs to in the evanescent field or the probability of colliding in the base is corresponding improves, and the nano particle of detection solution carries out strong collision and adhesion through high minimum microchannel space and with the base surface, makes the utility model discloses technical scheme thin layer flow cell can conveniently observe single nano particle and single fluorescent particle molecule in SPR microscope and total internal reflection fluorescence microscope, furthest improves fluorescent molecule and nano particle and reaches super high sensitivity.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
Fig. 1 is a schematic structural view of a thin layer flow cell according to example 1 of the present invention;
fig. 2 is a cross-sectional view showing the internal structure of a thin layer flow cell according to example 1 of the present invention;
fig. 3 is a flow chart of the manufacturing process of the thin layer flow cell of example 1 of the present invention;
fig. 4 is a cross-sectional view showing the internal structure of a thin layer flow cell according to example 2 of the present invention;
fig. 5 is a cross-sectional view showing the internal structure of a thin layer flow cell according to example 3 of the present invention;
fig. 6 is a sectional view showing the internal structure of a thin layer flow cell according to example 4 of the present invention;
fig. 7 is a cross-sectional view showing the internal structure of a thin layer flow cell according to example 5 of the present invention;
fig. 8 is a schematic diagram of the thin layer flow cell of the present invention;
FIG. 9 is a graph showing the relationship between the signal intensity of the nanoparticle detection and the height from the top surface of the substrate;
FIG. 10 is a graph showing the results of the number of exosomes detected by microchannels of different thicknesses at the same time and concentration.
The reference numbers illustrate:
| reference numerals | Name (R) | Reference numerals | Name (R) |
| 1 | |
3 | Connecting |
| 11 | Micro-channel | 31 | |
| 12 | Communicating |
32 | |
| 13 | Surrounding |
33 | |
| 2 | |
5 | Clamp apparatus |
| 21 | Groove structure |
The objects, features and advantages of the present invention will be further described with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that, if directional indications (such as upper, lower, left, right, front and rear … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indications are changed accordingly.
In addition, if there is a description relating to "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.
The utility model provides a thin layer flow cell of super high sensitivity detection unimolecule and single nanometer particle.
Example 1
Please refer to fig. 1 and fig. 2, the thin layer flow cell for detecting single molecule and single nano particle with ultra-high sensitivity of the present invention comprises a base 2, a flow cell main body 1 and a connecting pipe 3, wherein the bottom surface of the flow cell main body 1 and the top surface of the base 2, the middle position of the bottom surface of the flow cell main body 1 is provided with a concave micro channel 11, the flow cell main body 1 is provided with a communicating hole 12 extending from the surface to the inside of the flow cell main body 1 and communicating with the micro channel 11, and the connecting pipe 3 extends into the inside of the flow cell main body 1 through the communicating hole 12 and communicating with the micro channel 11. Preferably, the height of the microchannel 11 of the present embodiment ranges from 20nm to 100 um.
In order to make the base 2 and the flow cell main body 1 tightly fit, in the technical scheme of the present embodiment, after the base 2 and the flow cell main body 1 are connected up and down, the side portions of the two sides of the common structure are respectively clamped into the grooves of the clamp 5, so that the base 2 and the flow cell main body 1 can be clamped up and down.
In order to make the solution that is located 11 inside detection solutions of microchannel and the inside of connecting pipe 3 realize flowing more fast, this embodiment is through setting up the pump (not marking on the picture) to connecting pipe 3 includes feed liquor pipe 31 and drain pipe 32, the utility model discloses technical scheme pump's outlet end links to each other with feed liquor pipe 31, and the pump's inlet end links to each other with drain pipe 32. Preferably, the pump of this embodiment is a peristaltic pump, and the gears inside the peristaltic pump push the hoses to realize the rapid flow of the detection solution inside the liquid inlet pipe 31 and the liquid outlet pipe 32.
Preferably, the utility model discloses technical scheme base 2's material is ordinary glass, the top surface has plated gold or silver or the glass of platinum thin layer, ITO conductive glass, AZO conductive glass, the FTO conductive glass's of gold thin layer arbitrary one, and flow cell main part 1 material is polymethyl methacrylate, polydimethylsiloxane, epoxy, polyurethane, polyamide arbitrary one or more, and the curing agent that adopts in the flow cell main part 1 manufacturing process is SYLGARD 184 curing agent, ethylenediamine, m-phenylenediamine, imidazole arbitrary one or more.
The utility model discloses a thin layer flow cell produces through following step:
referring to fig. 1 to 3, a recessed structure is etched on a surface of a silicon wafer by a photolithography technique, a raised structure protruding upward is disposed at a center of the recessed structure, the raised structure may be a cuboid or other structures, a silicone rubber encapsulation liquid is added into the recessed structure on the surface of the etched silicon wafer to cover the silicone rubber encapsulation liquid inside the recessed structure and on an upper surface of the raised structure, and the silicon wafer covered with the silicone rubber encapsulation liquid is placed in an oven at a certain working temperature and dried for a certain time.
After the silicon wafer is dried, the silicon wafer mold and the cured silicon rubber are separated from each other, the cured silicon rubber becomes a flow cell main body, a micro-channel with the size consistent with that of the protruding structure is formed on the bottom surface of the flow cell main body, then communication holes with the same inner diameter are punched in the flow cell main body by a puncher, the communication holes are arranged from top to bottom and penetrate into the flow cell main body and are connected with the micro-channel, then the connecting pipe is inserted into the communication holes, and then the connecting pipe and the flow cell main body are bonded and connected by an adhesive.
Finally, the base and the side part of the main body of the flow cell are clamped and fixedly connected by adopting a clamp, then the nano particle detection solution is conveyed to the inside of the micro channel by using the pump body through a liquid inlet pipe of the connecting pipe, then the thin-layer flow cell loaded with the detection solution is placed on an SPR microscope detection platform, and the concentration of the detection solution can be detected with ultrahigh sensitivity by observing a single nano particle device in the detection solution and adhering the single nano particle device to the surface condition of the base.
Example 2
Referring to fig. 4, the following technical features are present between this embodiment and embodiment 1: the surface of the base 2 is provided with a groove structure 21, and the bottom of the flow cell main body 1 is at least partially embedded into the groove structure 21. Through being provided with groove structure 21 on base 2 surface to the size of the inboard border of groove structure 21 is the same with the border size in flow cell main part 1 bottom, thereby makes flow cell main part 1 bottom at least position structure imbed inside groove structure 21 on base 2 surface, prevents to take place relative slip between base 2 and the flow cell main part 1 in the testing process and influences and detect the accuracy. In addition, the clamp 5 with a groove inside can be used for clamping the flow cell main body 1 and the base 2 which are connected up and down, so that the connection reliability of the two is further improved.
Example 3
Referring to fig. 5, the following technical features are present in this embodiment and embodiment 1: the bottom edge of the flow cell main body 1 is provided with a downward surrounding edge structure 13, and the surrounding edge structure 13 is at least partially sleeved on the top of the base 2. Through the protruding structure that sets up downwards at flow cell main part 1 bottom surface border, also be surrounding structure 13 to surrounding structure 13's inside dimension is the same with base 2 top border dimension, thereby makes base 2 top at least partial structure can imbed to flow cell main part 1 inside, prevents to take place relative slip influence detection accuracy between base 2 and flow cell main part 1 in the testing process. In addition, the clamp 5 with a groove inside can be used for clamping the flow cell main body 1 and the base 2 which are connected up and down, so that the connection reliability of the two is further improved.
Example 4
Referring to fig. 6, the following technical features are present between this embodiment and embodiment 1: the bottom edge of the connecting pipe 3 is flush with the top surface of the micro-channel 11. By making the bottom edge of the connecting tube 3 flush with the top surface of the microchannel 11, the detection solution can smoothly flow out through the bottom end connection port of the connecting tube 3 or flow into the microchannel 11.
Example 5
Referring to fig. 7, the following technical features are present between this embodiment and embodiment 1: the edge of the bottom end of the connecting pipe 3 is flush with the bottom surface of the micro-channel 11, the bottom end of the connecting pipe 3 is provided with a notch 33 communicated with the micro-channel 11, and the height of the notch 33 is larger than or equal to the height of the micro-channel 11. By supporting the bottom end of the connection tube 3 on the top surface of the base 2, the connection tube 3 can be more reliably connected to the flow cell main body 1, and the detection solution can smoothly flow out through the bottom end connection port of the connection tube 3 or flow into the microchannel 11 through the notch 33 provided at the bottom end of the connection tube 3.
Example 6
In the embodiment, a concave structure is etched on the surface of a silicon wafer through a photoetching technology, a convex structure protruding upwards is arranged at the center of the concave structure, wherein the convex structure is a cuboid structure, the length, the width and the height of the convex structure are respectively 1cm multiplied by 0.1cm multiplied by 50 micrometers, then Dow Corning SYLGARD 184 silicon rubber packaging liquid is added into the concave structure on the surface of the etched silicon wafer, the weight ratio of the basic components of the silicon rubber packaging liquid to a curing agent is 10:1, the silicon rubber packaging liquid in the proportion is adopted to cover the inner part of the concave structure on the surface of the silicon wafer and the upper surface of the convex structure, and the silicon wafer covered with the silicon rubber packaging liquid is placed in an oven with the working temperature of 80 ℃ to be dried for 30 min.
After drying, separating the silicon wafer die and the cured silicon rubber from each other, wherein the cured silicon rubber becomes a flow cell main body, a silicon rubber micro-channel with the size consistent with that of the protruding structure is formed on the bottom surface of the flow cell main body, two communicating holes with the diameter of 1.5mm are respectively punched at two ends of the silicon rubber micro-channel through a puncher, and then a polytetrafluoroethylene hose with the outer diameter of 1.5mm is connected with the silicon rubber through epoxy resin glue.
Then the substrate and the flow cell main body made of silicon rubber are fixedly connected through a clamp, the gold nanoparticle detection solution is injected into the micro-channel through an injection pump, the thin-layer flow cell with the detection solution is placed on an SPR microscope detection platform, then the condition that a single gold nanoparticle in the detection solution collides and adheres to the surface of the substrate can be observed, and therefore ultrahigh-sensitivity detection can be carried out on the concentration of the detection solution.
Example 7
In the embodiment, a concave structure is etched on the surface of a silicon wafer through a photoetching technology, a convex structure protruding upwards is arranged at the center of the concave structure, the convex structure is of a cuboid structure, the length, the width and the height of the convex structure are respectively 1cm multiplied by 0.1cm multiplied by 10 micrometers, then Dow Corning SYLGARD 184 silicon rubber packaging liquid is added into the concave structure on the surface of the etched silicon wafer, the weight ratio of the basic components of the silicon rubber packaging liquid to a curing agent is 10:1, the silicon rubber packaging liquid in the proportion is adopted to cover the inner part of the concave structure on the surface of the silicon wafer and the upper surface of the convex structure, and the silicon wafer covered with the silicon rubber packaging liquid is placed in an oven with the working temperature of 65 ℃ to be dried for 4 hours.
After drying, separating the silicon wafer die and the cured silicon rubber from each other, wherein the cured silicon rubber becomes a flow cell main body, a silicon rubber micro-channel with the size consistent with that of the protruding structure is formed on the bottom surface of the flow cell main body, two communicating holes with the diameter of 1.5mm are respectively punched at two ends of the silicon rubber micro-channel through a puncher, and then a polytetrafluoroethylene hose with the outer diameter of 1.5mm is connected with the silicon rubber through epoxy resin glue.
Then the substrate and the flow cell main body made of silicon rubber are fixedly connected through a clamp, then the exosome detection solution is injected into the micro-channel through an injection pump, the thin-layer flow cell with the detection solution is placed on an SPR microscope detection platform, then the condition that a single nano particle in the detection solution collides and adheres to the surface of the substrate can be observed, and therefore the concentration of the detection solution can be detected with ultrahigh sensitivity.
Example 8
In the embodiment, a concave structure is etched on the surface of a silicon wafer through a photoetching technology, a convex structure protruding upwards is arranged at the center of the concave structure, the convex structure is of a cuboid structure, the length, the width and the height of the convex structure are respectively 1cm multiplied by 0.1cm multiplied by 2 mu m, then Dow Corning SYLGARD 184 silicon rubber packaging liquid is added into the concave structure on the surface of the etched silicon wafer, the weight ratio of the basic components of the silicon rubber packaging liquid to a curing agent is 10:1, the silicon rubber packaging liquid in the proportion is adopted to cover the inner part of the concave structure on the surface of the silicon wafer and the upper surface of the convex structure, and the silicon wafer covered with the silicon rubber packaging liquid is placed in an oven with the working temperature of 80 ℃ to be dried for 1 h.
After drying, separating the silicon wafer die and the cured silicon rubber from each other, wherein the cured silicon rubber becomes a flow cell main body, a silicon rubber micro-channel with the size consistent with that of the protruding structure is formed on the bottom surface of the flow cell main body, two communicating holes with the diameter of 1.5mm are respectively punched at two ends of the silicon rubber micro-channel through a puncher, and then a polytetrafluoroethylene hose with the outer diameter of 1.5mm is connected with the silicon rubber through epoxy resin glue.
Then the substrate and the flow cell main body made of silicon rubber are fixedly connected through a clamp, the gold nanoparticle detection solution is injected into the micro-channel through an injection pump, the thin-layer flow cell with the detection solution is placed on an SPR microscope detection platform, then the condition that a single gold nanoparticle in the detection solution collides and adheres to the surface of the substrate can be observed, and therefore ultrahigh-sensitivity detection can be carried out on the concentration of the detection solution.
Example 9
In the embodiment, a concave structure is etched on the surface of a silicon wafer through a photoetching technology, a convex structure protruding upwards is arranged at the center of the concave structure, the convex structure is a cuboid structure, the length, the width and the height of the convex structure are respectively 1cm multiplied by 0.1cm multiplied by 1 mu m, then Dow Corning SYLGARD 184 silicon rubber packaging liquid is added into the concave structure on the surface of the etched silicon wafer, the weight ratio of the basic components of the silicon rubber packaging liquid to a curing agent is 10:1, the silicon rubber packaging liquid in the proportion is adopted to cover the inner part of the concave structure on the surface of the silicon wafer and the upper surface of the convex structure, and the silicon wafer covered with the silicon rubber packaging liquid is placed in an oven with the working temperature of 80 ℃ to be dried for 1 h.
After drying, separating the silicon wafer die and the cured silicon rubber from each other, wherein the cured silicon rubber becomes a flow cell main body, a silicon rubber micro-channel with the size consistent with that of the protruding structure is formed on the bottom surface of the flow cell main body, two communicating holes with the diameter of 1.5mm are respectively punched at two ends of the silicon rubber micro-channel through a puncher, and then a polytetrafluoroethylene hose with the outer diameter of 1.5mm is connected with the silicon rubber through epoxy resin glue.
Then the substrate and the flow cell main body made of silicon rubber are fixedly connected through a clamp, then the exosome detection solution is injected into the micro-channel through an injection pump, the thin-layer flow cell with the detection solution is placed on an SPR microscope detection platform, then the condition that a single exosome in the detection solution collides and adheres to the surface of the substrate can be observed, and therefore ultrahigh-sensitivity detection can be carried out on the concentration of the detection solution.
Referring to fig. 1, fig. 2, fig. 8 to fig. 10, and combined with examples 1 and 6 to 9, after the thin layer flow cell of this embodiment is placed on the SPR microscope detection platform, the optical path is generated under the base 2 through the objective lens, and the situation that a single nanoparticle in the detection solution collides and adheres to the top surface of the base 2 can be conveniently observed through the SPR microscope.
Combine fig. 9, the utility model discloses among the technical scheme, along with the nanoparticle constantly increases apart from 2 top surfaces of basement, detect the fast decay of nanoparticle signal intensity, and the nanoparticle highly tends to zero apart from 2 top surfaces of basement, detects the nanoparticle signal intensity and is the biggest and tend to infinity.
In addition, referring to FIG. 10, it can be understood that the concentration of the detection solution is the same and the detection time is the same, for example, 1 min. Along with microchannel highly constantly reduces, the individual number that detects the exosome constantly improves, the utility model discloses technical scheme's super high sensitivity detects the thin layer flow cell of monomolecular and single nanometer particle can carry out super high sensitivity to the concentration that detects solution and detect.
The above only is the preferred embodiment of the present invention, not so limiting the patent scope of the present invention, all under the concept of the present invention, the equivalent structure transformation made by the contents of the specification and the drawings is utilized, or the direct/indirect application is included in other related technical fields in the patent protection scope of the present invention.
Claims (10)
1. The utility model provides an ultra-high sensitive detection monomolecular and single nano particle's thin layer flow cell, its characterized in that includes base, flow cell main part and connecting pipe, and flow cell main part sets up in the base top surface, and flow cell main part bottom surface is equipped with indent structure and constitutes the microchannel with the base top surface, and flow cell main part is equipped with the intercommunicating pore and stretches into to flow cell main part inside and lead to with the microchannel from the surface, and the connecting pipe stretches into flow cell main part inside and leads to with the microchannel through the intercommunicating pore.
2. The thin layer flow cell of claim 1, wherein the microchannel height ranges from 20nm to 100 um.
3. The thin layer flow cell of claim 1, wherein the base surface is provided with a groove structure, and the bottom of the flow cell body is at least partially embedded in the groove structure.
4. The thin layer flow cell of claim 1, wherein the bottom edge of the flow cell body is provided with a downwardly disposed perimeter structure that at least partially nests over the top of the base.
5. The thin-layer flow cell of any one of claims 1, 3 and 4, wherein after the base and the flow cell body are connected up and down, the two side portions are respectively clamped into the grooves of the clamps to clamp the base and the flow cell body up and down.
6. The thin layer flow cell of claim 1, wherein the bottom end edge of the connecting tube is flush with the top surface of the microchannel.
7. The thin layer flow cell of claim 1, wherein the bottom edge of the connecting tube is flush with the bottom surface of the microchannel, and the bottom end of the connecting tube is provided with a gap communicating with the microchannel, and the gap has a height greater than or equal to the height of the microchannel.
8. The thin layer flow cell of claim 6 or 7, wherein the connection tubes comprise an inlet tube and an outlet tube, the inlet tube and/or the outlet tube being connected to a pump.
9. The thin layer flow cell of claim 8, wherein the pump is a peristaltic pump.
10. The thin-layer flow cell of claim 1, wherein the base is made of any one of common glass, glass coated with gold, silver or platinum on the top surface, ITO conductive glass, AZO conductive glass, and FTO conductive glass, the flow cell body is made of any one of polymethyl methacrylate, polydimethylsiloxane, epoxy resin, polyurethane, and polyamide, the curing agent used in the manufacturing process of the flow cell body is any one of SYLGARD 184 curing agent, ethylenediamine, m-phenylenediamine, and imidazole, and the connecting pipe is made of polytetrafluoroethylene.
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| CN111847905A (en) * | 2020-07-09 | 2020-10-30 | 南昌航空大学 | Preparation method of thin-layer flow cell for ultraviolet and visible light absorption test |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111847905A (en) * | 2020-07-09 | 2020-10-30 | 南昌航空大学 | Preparation method of thin-layer flow cell for ultraviolet and visible light absorption test |
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