CN111847905A - Preparation method of thin-layer flow cell for ultraviolet and visible light absorption test - Google Patents

Preparation method of thin-layer flow cell for ultraviolet and visible light absorption test Download PDF

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Publication number
CN111847905A
CN111847905A CN202010657306.XA CN202010657306A CN111847905A CN 111847905 A CN111847905 A CN 111847905A CN 202010657306 A CN202010657306 A CN 202010657306A CN 111847905 A CN111847905 A CN 111847905A
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China
Prior art keywords
quartz glass
polytetrafluoroethylene sheet
ultraviolet
visible light
preparation
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Pending
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CN202010657306.XA
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Chinese (zh)
Inventor
姜海燕
刘伟庆
范瑜鑫
毛志敏
蔡宏峰
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Nanchang Hangkong University
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Nanchang Hangkong University
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Priority to CN202010657306.XA priority Critical patent/CN111847905A/en
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    • C—CHEMISTRY; METALLURGY
    • C03—GLASS; MINERAL OR SLAG WOOL
    • C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
    • C03C27/00—Joining pieces of glass to pieces of other inorganic material; Joining glass to glass other than by fusing
    • C03C27/06—Joining glass to glass by processes other than fusing
    • C03C27/10—Joining glass to glass by processes other than fusing with the aid of adhesive specially adapted for that purpose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0332—Cuvette constructions with temperature control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0378—Shapes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/054—Bubble trap; Debubbling

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Ceramic Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Geochemistry & Mineralogy (AREA)
  • Materials Engineering (AREA)
  • Organic Chemistry (AREA)
  • Optical Measuring Cells (AREA)

Abstract

The invention discloses a preparation method of a thin-layer flow cell for ultraviolet and visible light absorption tests, which can be measured by utilizing UV-VIS (ultraviolet-visible light), can distinguish the real-time dynamic adsorption condition of a single molecule when various molecules are adsorbed on a thin film, and adopts quartz glass and a polytetrafluoroethylene sheet as main materials, so that the preparation method is low in cost, simple to prepare and reusable, triangular pointed grooves with upward angles are engraved at the top of a flow cavity, triangular pointed grooves with downward angles are engraved at the bottom of the flow cavity, bubbles can be conveniently discharged in the experimental process, the influence of bubbles is small during the test, a temperature control cavity is communicated with a water bath, and the control of the adsorption environment temperature can be realized by adjusting the temperature of the water bath, so that the constant-temperature experimental condition is achieved.

Description

Preparation method of thin-layer flow cell for ultraviolet and visible light absorption test
Technical Field
The invention relates to the crossing field of an ultraviolet testing technology, a quartz microbalance flow cell testing technology and a flow cell temperature control technology, in particular to a preparation method of a thin-layer flow cell for ultraviolet and visible light absorption testing.
Background
In the research of a photoanode of a dye-sensitized solar cell (DSC), it is important to research and analyze the performance of the cell to explore the adsorption kinetics of dye molecules and co-adsorbent molecules on a nano-film.
The traditional test method is an ultraviolet-visible light photometer (UV-VIS) measurement method, namely, a sensitized membrane which is adsorbed and saturated in a static state is desorbed by using alkali liquor, the dye content in the alkali liquor is measured, the sensitized membrane can be saturated after being soaked for 8 to 12 hours, the measured adsorption capacity is the final adsorption capacity obtained after long-time reaction on the membrane, and the real-time adsorption process of the dye on the membrane cannot be detected.
Another measurement method is to monitor the dynamic change of the dye adsorption amount on the film in real time by using a quartz microbalance (QCM) and a flow cell, but when the dye and the co-adsorbent molecules are adsorbed on the film simultaneously, the frequency is reflected by the sum of the two molecular weights on the film, and the adsorption condition of the single dye molecule cannot be distinguished.
Therefore, the invention aims to invent a thin-layer flow cell for ultraviolet testing, which can realize real-time monitoring of dynamic adsorption condition of one colored molecule when two or more molecules are adsorbed on a nano film under a constant temperature condition.
Disclosure of Invention
The invention aims to solve at least one of the technical problems in the prior art, and provides a preparation method of a thin-layer flow cell, which can be used for ultraviolet and visible light tests and has the function of self temperature control, and aims to obtain a method for monitoring the adsorption process of one colored molecule on a nano-film when a plurality of molecules are simultaneously adsorbed on the film at constant temperature in real time.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
a preparation method of a thin-layer flow cell for ultraviolet and visible light absorption tests comprises a first quartz glass, a second quartz glass, a third quartz glass, a first polytetrafluoroethylene sheet and a second polytetrafluoroethylene sheet,
step (ii) of1, the first quartz glass, the second quartz glass and the third quartz glass are all cleaned by ultrasonic cleaning, and a layer of nano-porous T is blade-coated on the third quartz glassiO2After the film is formed, sintering the film and the first quartz glass, the second quartz glass and the third quartz glass for 30min at 480 ℃ in a muffle furnace;
step 2, engraving a nano-porous T for the step 1 at the middle position of the first polytetrafluoroethylene sheetiO2The hole through which the film penetrates is used as a flow cavity for solution, a triangular pointed groove with an upward angle is carved at the top of the flow cavity, and a triangular pointed groove with a downward angle is carved at the bottom of the flow cavity for discharging bubbles in the experimental process;
step 3, engraving a 'U' -shaped groove in the middle position of the second polytetrafluoroethylene sheet to serve as a temperature control cavity for water bath circulation;
step 4, assembling the first quartz glass, the second polytetrafluoroethylene sheet, the second quartz glass, the first polytetrafluoroethylene sheet and the third quartz glass in sequence, wherein the first polytetrafluoroethylene sheet and the second polytetrafluoroethylene sheet are embedded into a catheter;
And 5, packaging the assembly obtained in the step 4 by using water-proof glue with stable chemical performance, strong plasticity and high viscosity, and fixing by using a frame.
The invention has the beneficial effects that:
1. the real-time dynamic adsorption condition of a single molecule can be distinguished when various molecules are adsorbed on the film;
2. quartz glass and a polytetrafluoroethylene sheet are used as main materials, so that the cost is low, the preparation is simple, and the material can be repeatedly used;
3. the triangular pointed grooves with upward angles are carved at the top of the flow cavity, the triangular pointed grooves with downward angles are carved at the bottom of the flow cavity, so that bubbles can be conveniently discharged in the experimental process, and the influence of the bubbles is small during testing;
4. the temperature control cavity is communicated with the water bath, so that the flowing pool can realize the self temperature control function.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic view of the overall structure of the present invention;
description of reference numerals:
the device comprises a first quartz glass 1, a second quartz glass 2, a third quartz glass 3, a first polytetrafluoroethylene sheet 4, a second polytetrafluoroethylene sheet 5, a flow cavity 6 and a temperature control cavity 7.
Detailed Description
Reference will now be made in detail to the present preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
Referring to fig. 1, a preferred embodiment of the present invention, a method for manufacturing a thin layer flow cell for uv-vis absorption test, includes a first quartz glass 1, a second quartz glass 2, a third quartz glass 3, a first teflon sheet 4, and a second teflon sheet 5,
step 1, ultrasonically cleaning a first quartz glass 1, a second quartz glass 2 and a third quartz glass 3, and blade-coating a layer of nano-porous T on the third quartz glass 3iO2After the film is formed, the film is sintered with the first quartz glass 1, the second quartz glass 2 and the third quartz glass 3 for 30min at 480 ℃ in a muffle furnace;
step 2, engraving a nano-porous T for the step 1 at the middle position of the first polytetrafluoroethylene sheet 4iO2The hole through which the film penetrates is used as a flow cavity 6 for solution, a triangular pointed groove with an upward angle is carved at the top of the flow cavity 6, and a triangular pointed groove with a downward angle is carved at the bottom of the flow cavity 6 for discharging bubbles in the experimental process;
step 3, engraving a 'return' shaped groove in the middle position of a second polytetrafluoroethylene sheet 5 to serve as a temperature control cavity 7 for water bath circulation;
step 4, assembling the first quartz glass 1, the second polytetrafluoroethylene sheet 5, the second quartz glass 2, the first polytetrafluoroethylene sheet 4 and the third quartz glass 3 in sequence, wherein the first polytetrafluoroethylene sheet 4 and the second polytetrafluoroethylene sheet 5 are both embedded into a catheter;
Step 5, the assembly in step 4 is packaged by water-proof glue with stable chemical property, strong plasticity and high viscosity, is fixed by a clamp, can be measured by UV-VIS, and can distinguish the real-time dynamic adsorption condition of a single molecule when various molecules are adsorbed on the film, and the invention adopts quartz glass and polytetrafluoroethylene sheets as main materials, has low cost, simple preparation and reusability, the top of the flow cavity 6 is engraved with an upward triangular pointed groove and the bottom of the flow cavity 6 is engraved with a downward triangular pointed groove, which is convenient for air bubble discharge in the experimental process, has small influence by air bubbles during test, the temperature control cavity 7 is communicated with a water bath, and the control of the adsorption environment temperature can be realized by adjusting the temperature of the water bath, so as to achieve the constant temperature experimental condition,specifically, the first quartz glass 1, the second quartz glass 2 and the third quartz glass 3 are 35mm long, 25mm wide and 2mm thick; the third quartz glass 3 is coated with a nano-porous TiO2The diameter of the film is 13mm, and the thickness of the film is 2.5 mu m; the first polytetrafluoroethylene sheet 4 and the second polytetrafluoroethylene sheet 5 are the same size as the first quartz glass 1 and/or the second quartz glass 2 and/or the third quartz glass 3; the first polytetrafluoroethylene sheet 4 and the second polytetrafluoroethylene sheet 5 have a thickness of 1 mm.
The above additional technical features can be freely combined and used in superposition by those skilled in the art without conflict.
The above description is only a preferred embodiment of the present invention, and the technical solutions that achieve the objects of the present invention by basically the same means are all within the protection scope of the present invention.

Claims (1)

1. A preparation method of a thin-layer flow cell for ultraviolet and visible light absorption tests is characterized by comprising the following steps: comprises a first quartz glass (1), a second quartz glass (2), a third quartz glass (3), a first polytetrafluoroethylene sheet (4) and a second polytetrafluoroethylene sheet (5),
step 1, ultrasonically cleaning a first quartz glass (1), a second quartz glass (2) and a third quartz glass (3), and blade-coating a layer of nano-porous T on the third quartz glass (3)iO2After the film is formed, sintering the film and the first quartz glass (1), the second quartz glass (2) and the third quartz glass (3) in a muffle furnace at 480 ℃ for 30 min;
step 2, engraving a nano-porous T for the step 1 at the middle position of the first polytetrafluoroethylene sheet (4)iO2The hole through which the film penetrates is used as a flow cavity (6) for solution, a triangular pointed groove with an upward carved angle is formed in the top of the flow cavity (6), and a triangular pointed groove with a downward carved angle is formed in the bottom of the flow cavity (6) so as to discharge air bubbles in the experimental process;
Step 3, engraving a square groove in the middle of the second polytetrafluoroethylene sheet (5) to serve as a temperature control cavity (7) for water bath circulation;
step 4, assembling the first quartz glass (1), the second polytetrafluoroethylene sheet (5), the second quartz glass (2), the first polytetrafluoroethylene sheet (4) and the third quartz glass (3) in sequence, wherein the first polytetrafluoroethylene sheet (4) and the second polytetrafluoroethylene sheet (5) are embedded into a catheter;
and 5, packaging the assembly obtained in the step 4 by using water-proof glue with stable chemical performance, strong plasticity and high viscosity, and fixing by using a clamp.
CN202010657306.XA 2020-07-09 2020-07-09 Preparation method of thin-layer flow cell for ultraviolet and visible light absorption test Pending CN111847905A (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3132926A1 (en) * 1980-11-18 1982-07-01 Veb Kombinat Medizin- Und Labortechnik Leipzig, Ddr 7035 Leipzig Temperature-controllable cuvette
CN1721056A (en) * 2005-05-26 2006-01-18 上海交通大学 Photoelectrocatalytic thin-layered minisize reactor
CN201083727Y (en) * 2007-08-23 2008-07-09 上海利穗电子科技有限公司 Detector flow cell for preparing color spectrum chromatography system
CN104390941A (en) * 2014-11-03 2015-03-04 大连理工大学 Method for testing photochemical reaction of solid-liquid interface by using cuvette
WO2015175189A1 (en) * 2014-05-15 2015-11-19 General Electric Company Microfluidic flow cell assemblies and method of use
CN106645339A (en) * 2016-12-28 2017-05-10 长春鼎诚科技有限公司 Thin-layer flow type photoelectric detector and oxidation resisting capacity detection method
CN110325830A (en) * 2017-02-23 2019-10-11 锋翔科技公司 Integrated irradiating and detecting flow cell for liquid chromatogram
CN210166310U (en) * 2019-05-28 2020-03-20 南京医科大学 Thin-layer flow cell for detecting single molecules and single nanoparticles with ultrahigh sensitivity

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3132926A1 (en) * 1980-11-18 1982-07-01 Veb Kombinat Medizin- Und Labortechnik Leipzig, Ddr 7035 Leipzig Temperature-controllable cuvette
CN1721056A (en) * 2005-05-26 2006-01-18 上海交通大学 Photoelectrocatalytic thin-layered minisize reactor
CN201083727Y (en) * 2007-08-23 2008-07-09 上海利穗电子科技有限公司 Detector flow cell for preparing color spectrum chromatography system
WO2015175189A1 (en) * 2014-05-15 2015-11-19 General Electric Company Microfluidic flow cell assemblies and method of use
CN104390941A (en) * 2014-11-03 2015-03-04 大连理工大学 Method for testing photochemical reaction of solid-liquid interface by using cuvette
CN106645339A (en) * 2016-12-28 2017-05-10 长春鼎诚科技有限公司 Thin-layer flow type photoelectric detector and oxidation resisting capacity detection method
CN110325830A (en) * 2017-02-23 2019-10-11 锋翔科技公司 Integrated irradiating and detecting flow cell for liquid chromatogram
CN210166310U (en) * 2019-05-28 2020-03-20 南京医科大学 Thin-layer flow cell for detecting single molecules and single nanoparticles with ultrahigh sensitivity

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