CN210154903U - Sample extraction and separation device controlled by pressure difference and sample processing device - Google Patents
Sample extraction and separation device controlled by pressure difference and sample processing device Download PDFInfo
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- CN210154903U CN210154903U CN201821996830.4U CN201821996830U CN210154903U CN 210154903 U CN210154903 U CN 210154903U CN 201821996830 U CN201821996830 U CN 201821996830U CN 210154903 U CN210154903 U CN 210154903U
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Abstract
The utility model discloses a sample extraction and separation device and sample processing apparatus of poor control of pressurized. The sample extraction and separation device controlled by the pressure difference comprises: the main body comprises a cavity, and the hydrophobic sieve plate is arranged in the cavity and used for preventing an object above the sieve plate from falling; an opening is formed in the bottom of the main body; when the sample is extracted, the hydrophobic sieve plate is used for preventing the permeation of an extraction solvent; when the sample is separated, when a pressure difference exists between two sides of the hydrophobic sieve plate, the sample components to be separated can pass through the hydrophobic sieve plate. Utilize the sample extraction separator that this application provided, can need not centrifugal operation after extracting the operation, only filter through applying pressure differential and just can separate pending sample completely effectively, saved operating time, when having avoided empting after the centrifugation moreover, the upper strata is sneaked into to the sample of lower floor.
Description
Technical Field
The utility model relates to an analytical chemistry technical field, concretely relates to sample extraction separator of poor control of pressurized.
Background
The detection of chemical and biological components in human body, food and environment has become a content closely related to the daily life of people. This includes measurement of the blood concentration of the drug, measurement of metabolites in urine after the patient has taken the drug; measuring pesticide residue or other harmful substances in food (such as malachite green and Sudan red), and measuring nutrient substances; and (4) measuring harmful substances in water and air. In all the detection processes, no matter what analytical instrument technology is used for detection, most of the detection processes need to be performed by sample pretreatment, including sample extraction, enrichment and purification and other related processes.
Currently, the commonly used sample pretreatment products based on the adsorbent mainly comprise a solid phase extraction column and a QuEChERS form.
The solid phase extraction column type is in the form of a syringe column tube, an upper porous sieve plate and a lower porous sieve plate are additionally arranged in the column tube, and a granular adsorbent which cannot be loosened is clamped between the two sieve plates. In the solid phase extraction process, the solid phase extraction column is activated, then a sample solution extracted in advance in another container is loaded on an adsorbent in the column, impurities are removed by leaching, and finally a required target object is washed by using a specific elution solution. Therefore, the whole solid phase extraction operation needs to extract the sample in another container in advance, the operation process is relatively complex, the required time is long, and the requirement on the technical level of personnel is high.
QuEChERS is a combination of partial abbreviations for Quick (Quick), simple (Easy), Cheap (Cheap), efficient (Effective), durable (Rugged) and Safe (Safe) words. The method is a convenient and efficient purification pretreatment technology for detecting pesticide residues in agricultural products. With the development of the field of QuEChERS use, the method becomes a standard solid-liquid separation method for detecting pesticide residues in fruits and vegetables globally. In addition, along with the development of the application, the method also relates to more and more fields, such as detection of veterinary drugs in meat, environmental water samples, wine, pollutants in soil, drugs, abuse drugs and the like.
QuEChERS has the characteristics of simple program, high efficiency and high sensitivity. However, the prior QuEChERS technology is mainly carried out in a centrifugal tube, and in an extraction or purification link, an adsorbent in the centrifugal tube is required to be mixed with an extraction solution or a purification solution through oscillation, and then solid-liquid separation of the solid adsorbent and the solution is realized through centrifugation. However, the following disadvantages are present in the centrifugal treatment: 1. the centrifugal operation is relatively complicated; 2. the centrifugation process takes a long time; 3. during solid-liquid separation after centrifugation, adsorbent particles enter the upper layer of liquid.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention is directed to a sample extraction and separation device, which can extract and/or purify a sample quickly and effectively and prevent other solid particles such as an adsorbent from entering into a separated liquid.
An embodiment of the utility model provides a sample extraction and separation device, it includes: the main body comprises a cavity, the hydrophobic sieve plate is arranged in the cavity, and the hydrophobic sieve plate is used for preventing an object above the sieve plate from falling; an opening is formed in the bottom of the main body; when the sample is extracted, the hydrophobic sieve plate is used for preventing the extraction solvent from passing through; when the sample is separated, when a pressure difference exists between two sides of the hydrophobic sieve plate, the sample components to be separated can pass through the hydrophobic sieve plate. That is, when there is no pressure difference on the two sides of the hydrophobic sieve plate, the solid, liquid, suspended matter and the like above the hydrophobic sieve plate can not pass through the sieve plate, and the hydrophobic sieve plate and the cavity form a container for containing the sample to be processed and the like.
The hydrophobic sieve plate in the embodiment of the utility model is used for preventing the extraction solvent from permeating; wherein the extraction solvent is water, or strong polar organic solvent such as methanol, acetonitrile, etc., or their mixture solution.
The hydrophobic sieve plate is: in the absence of a pressure difference, the above-mentioned extraction solvent cannot pass through the sieve plate.
The embodiment of the utility model provides an in the sample separation, refer to separate the solid that sample residue, adsorbent formed and the liquid of extraction solvent.
In one embodiment of the present invention, the hydrophobic sieve plate is made of porous polytetrafluoroethylene. In this embodiment, the position of the hydrophobic screen deck in the cavity is not limited, for example, the distance of the hydrophobic screen deck from the opening is less than the height of 2/3, 1/2, 1/3 or 1/5 of the body, or the hydrophobic screen deck is disposed at the bottom of the body.
In a specific embodiment of the present invention, the pore size of the hydrophobic sieve plate is 2 μm to 20 μm, preferably, the pore size of the hydrophobic sieve plate is 2 μm, 5 μm, 8 μm, 10 μm, 12 μm, 15 μm, 18 μm or 20 μm.
In a particular embodiment of the present invention, the hydrophobic screen has a thickness of 0.5mm to 5 mm. The sieve plate with the thickness of 0.5mm-5mm is more favorable for blocking objects above the sieve plate from falling on one hand, and is favorable for filtering on the other hand. Preferably, the hydrophobic screen has a thickness of 0.5mm, 0.8mm, 1mm, 1.2mm, 1.5mm, 1.7mm, 2mm, 2.2mm, 2.5mm, 2.8mm, 3mm, 3.3mm, 3.5mm, 3.7mm, 4mm, 4.2mm, 4.5mm, 4.8mm, 5mm, or the like. More preferably, the hydrophobic screen has a thickness of 3mm or 5 mm.
In a specific embodiment of the present invention, the opening is not particularly limited, and the manner of forming the opening in the prior art is applicable to this embodiment. Preferably, the opening is formed by extending outwardly through a bottom of the body.
In a specific embodiment of the present invention, the sample extraction and separation apparatus further comprises a filtration membrane disposed below the hydrophobic sieve plate for filtering the liquid passing through the sieve plate. The filtering membrane can be arranged close to the sieve plate, and a certain distance can also exist between the filtering membrane and the sieve plate, and the specific arrangement can be determined according to specific requirements.
In one embodiment of the present invention, the pore size of the filtration membrane is 0.2 μm to 1 μm. Preferably, the pore size of the filtration membrane is 0.2 μm, 0.22 μm, 0.25 μm, 0.45 μm, 0.5 μm, 0.75 μm, 0.85 μm or 1 μm. More preferably, the pore size of the filtration membrane is 0.22 μm.
In a specific embodiment of the present invention, there is no special limitation on the material of the filtering membrane in this embodiment, and the membrane that can perform the filtering function in the prior art may be used. Preferably, the material of the filtering membrane is selected from the group consisting of polypropylene, polyethersulfone, nylon, polyvinylidene fluoride, polytetrafluoroethylene.
In a specific embodiment of the utility model, the sample extraction and separation device further comprises an adsorbent layer, the adsorbent layer is arranged above the hydrophobic sieve plate and is used for adsorbing impurities in the sample treatment process. The adsorbent layer can be tightly attached to the upper part of the sieve plate and can also have a certain distance with the sieve plate; the adsorbent layer can be directly fixed above the sieve plate, or the adsorbent can be directly paved above the sieve plate to form the adsorbent layer when in use.
In one embodiment of the invention, the adsorbent of the adsorbent layer may be selected according to the sample to be treated. Preferably, the adsorbent is selected from one or more of anhydrous sodium sulfate, anhydrous magnesium sulfate, sodium acetate, sodium chloride, C18 bonded silica gel, graphitized carbon (PC), N-propyl ethylene diamine (PSA) bonded silica gel, and polystyrene divinyl benzene high polymer.
In one embodiment of the present invention, the adsorbent layer is used in combination with the filtering membrane to further remove impurities in the sample.
In a particular embodiment of the present invention, a sealing cover is disposed above the main body for sealing the main body. When the body is sealed, the sample extraction and separation device may be used as a common container, for example similar to an EP tube, for sample mixing, shaking, etc. When the sealing cover is opened, a positive pressure is applied to the upper part of the sieve plate, or a negative pressure is applied to the lower part of the sieve plate, the sample components needing to be separated in the sample to be processed pass through the sieve plate, and then the sample separation is realized.
The utility model discloses another aspect provides a sample processing apparatus, sample processing apparatus includes foretell sample extraction separator and pressure device, pressure device does sample extraction separator provides pressure differential. Preferably, the pressure difference is 0.01MPa to 0.1MPa, for example, the pressure difference is 0.01MPa, 0.02MPa, 0.03MPa, 0.04MPa, 0.05MPa, 0.06MPa, 0.07MPa, 0.08MPa, 0.09MPa, or 0.1MPa, etc.
In one embodiment of the present invention, the pressure device is a vacuum pump, and the vacuum pump provides a vacuum condition for the sample extraction and separation device. For example, the sample extraction and separation device is connected with a sealed container in a matching way through an opening to form a closed space, and the sealed container is connected with a vacuum pump through a pipeline. When used for sample processing, the vacuum pump provides a negative pressure to the sample extraction and separation device.
In a specific embodiment of the present invention, the pressure device is a positive pressure device, and the positive pressure device provides a pressure difference when separating the sample. For example, the positive pressure assembly may include a booster sealingly coupled to the body portion of the sample processing assembly, the booster being slidable along the inner wall of the body. When processing a sample, the booster slides down along the inner wall of the main body to provide a positive pressure to the sample extraction and separation device.
The utility model discloses another aspect provides the application of hydrophobicity sieve in sample extraction and separation device or sample separation process.
In one embodiment of the present invention, the hydrophobic sieve plate is made of porous polytetrafluoroethylene.
In another aspect, the present invention provides a method for sample separation, which comprises processing a sample by the above sample extraction and separation device or by a sample processing device.
The utility model provides a sample extraction and separation device has one of following beneficial effect at least:
the utility model provides a sample extraction and separation device controlled by pressure difference, when no pressure difference exists, a hydrophobic sieve plate is used for preventing an object above the sieve plate from falling; the hydrophobic sieve plate serves to allow liquid above the sieve plate to pass while preventing solid particles from passing when there is a pressure differential. Utilize the sample extraction separator that this application provided, can need not centrifugal operation after the extraction operation, only filter through applying pressure differential and can separate pending sample completely effectively, saved operating time, when having avoided empting after the centrifugation moreover, the sample of lower floor mixes the upper strata, perhaps does not mix the upper strata for the sample that makes the lower floor and has to abandon the waste that part upper strata sample caused, be particularly useful for the less processing of sample volume.
Drawings
Fig. 1 is a schematic structural diagram of a longitudinal section of a sample extraction and separation device according to an embodiment of the present invention.
Fig. 2 is a schematic structural diagram of a longitudinal section of a sample extraction and separation device according to an embodiment of the present invention.
Fig. 3 is a schematic structural diagram of a longitudinal section of a sample extraction and separation device according to an embodiment of the present invention.
Fig. 4 is a schematic structural diagram of a longitudinal section of a sample extraction and separation device according to an embodiment of the present invention.
Fig. 5 is a schematic structural diagram of a longitudinal section of a sample extraction and separation device according to an embodiment of the present invention.
Fig. 6 is a schematic structural diagram of a sample processing device according to an embodiment of the present invention.
Fig. 7 is a schematic structural diagram of a sample processing device according to an embodiment of the present invention.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only some embodiments of the invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Example 1
As shown in fig. 2 and 3, the present embodiment provides a sample extraction separation apparatus including a main body 100 and a hydrophobic sieve plate 300. The main body 100 contains a cavity 101, and a hydrophobic screening deck 300 is disposed in the cavity 101 for blocking an object above the hydrophobic screening deck 300 from falling. The bottom of the body 100 is provided with an opening 102 for releasing the separated sample components.
When a sample is separated using the above-described sample extraction separation apparatus, when a pressure difference exists between both sides of the hydrophobic frit 300, a portion of the sample that can be separated may pass through the hydrophobic frit 300 and be released through the opening 102. The pressure differential in this embodiment may be created by applying a positive pressure over hydrophobic frit 300, such as by providing a booster above hydrophobic frit 300 that mates with cavity 101; it may also be formed by applying a negative pressure below the hydrophobic frit 300, for example by connecting the sample extraction and separation device to a vacuum pump through the opening 102.
The material, shape and size of the body 100 of the sample extraction and separation device in this embodiment are not limited, and may be, for example, polypropylene, and the inner diameter of the body is circular and is 1cm to 5 cm.
The hydrophobic sieve plate 300 in this embodiment is a hydrophobic sieve plate, and the material thereof may be a porous teflon sieve plate material. The position of the hydrophobic screening deck 300 in the cavity 101 is not limited, for example, the hydrophobic screening deck 300 is located at a distance less than 2/3, 1/2, 1/3 or 1/5 from the opening 102, etc., or the hydrophobic screening deck 300 is disposed at the bottom of the body 100.
The aperture and thickness of the hydrophobic frit 300 determines whether an object above the hydrophobic frit 300 can pass through the hydrophobic frit 300. In this embodiment, the aperture of the hydrophobic sieve plate 300 is 5 μm to 20 μm, and the thickness is 0.5mm to 5 mm. Preferably, the pore size of the hydrophobic sieve plate 300 is 5 μm, 8 μm, 10 μm, 12 μm, 15 μm, 18 μm or 20 μm; the thickness is 0.5mm, 0.8mm, 1mm, 1.2mm, 1.5mm, 1.7mm, 2mm, 2.2mm, 2.5mm, 2.8mm, 3mm, 3.3mm, 3.5mm, 3.7mm, 4mm, 4.2mm, 4.5mm, 4.8mm, 5mm or the like. More preferably, the hydrophobic screen has a thickness of 3mm or 5 mm.
The opening 102 is not particularly limited in this embodiment, and may be formed by extending the bottom of the body 100 outward. The extended portion may be connected to a vacuum device. Preferably, the bottom of the body 100 is extended to form a "V" shape (as shown in fig. 2) or a "U" shape (as shown in fig. 1) in a longitudinal section.
Example 2
As shown in fig. 4, in the present embodiment, the sample extraction and separation apparatus includes a main body 100, a hydrophobic sieve plate 300, and an adsorbent layer 200. The hydrophobic sieve plate 300 is disposed at the bottom of the main body 100, the adsorbent layer 200 is disposed above the hydrophobic sieve plate 300 for adsorbing impurities in a sample to be processed, and the bottom of the main body 100 extends outward to form an opening 102. The distance between the adsorbent layer 200 and the hydrophobic sieve plate 300 can be set according to actual needs. The adsorbent layer 200 may be directly fixed to the inner wall of the main body 100, or when used, the adsorbent may be directly laid on the hydrophobic sieve plate 300 to form the adsorbent layer 200, and an appropriate adsorbent may be selected according to the sample to be processed.
The adsorbent of the adsorbent layer in this embodiment may be selected according to the sample to be treated, for example, one or more of anhydrous sodium sulfate, anhydrous magnesium sulfate, sodium acetate, sodium chloride, C18-bonded silica gel, graphitized carbon (PC), N-propyl ethylenediamine (PSA) -bonded silica gel, polystyrene divinyl benzene high polymer.
Example 3
As shown in fig. 5, in the present embodiment, the sample extraction and separation apparatus includes a main body 100, a hydrophobic sieve plate 300, and a filtering membrane 400. Hydrophobic frit 300 is disposed within cavity 101 of body 100, filter membrane 400 is disposed below hydrophobic frit 300, and the bottom of body 100 extends outward to form opening 102. The filter membrane 400 may be directly adjacent to the hydrophobic sieve plate 300, or may be spaced apart from the hydrophobic sieve plate 300, and the specific arrangement is performed according to actual needs. The pore diameter of the filter membrane is 0.2-1 μm. Preferably, the pore size of the filtration membrane is 0.2 μm, 0.22 μm, 0.25 μm, 0.45 μm, 0.5 μm, 0.75 μm, 0.85 μm or 1 μm. More preferably, the pore size of the filtration membrane is 0.22 μm. In the embodiment, the filtering membrane can be selected from polypropylene, polyethersulfone, nylon, polyvinylidene fluoride, polytetrafluoroethylene and the like.
Example 4
As shown in fig. 1, in another embodiment of the present application, the sample extraction and separation apparatus includes a main body 100, and a hydrophobic sieve plate 300, the hydrophobic sieve plate 300 is disposed in a bottom cavity 101 of the main body 100, an adsorbent layer 200 is disposed above the hydrophobic sieve plate 300, a filter membrane 400 is disposed below the hydrophobic sieve plate 300, and a bottom of the main body 100 extends outward to form an opening 102. The adsorbent layer in this embodiment is used in combination with the filter membrane, and can further remove impurities in the sample.
The sample-extracting and-separating apparatus in each of the above embodiments may further include a sealing cap 400. The following description will be made by taking fig. 1 as an example. The sealing cover 400 is disposed above the main body 100 to seal the main body 100. The sealing cover 400 may be integrally formed with the body 100, or the sealing cover 400 may be separately provided. In performing the sample separation, the sealing cap 400, the hydrophobic frit 300, and the cavity 101 between the hydrophobic frit 300 and the sealing cap 400 form a sealed container for holding the sample and performing a processing operation on the sample, such as shaking, mixing, and the like. Of course, when the amount of the sample to be mixed is small, the sealing cap 400 is not necessary, and the sample extraction and separation device described above can be used as it is to perform shaking mixing or the like.
Example 5
As shown in fig. 6, the present embodiment provides a sample processing apparatus, which includes a main body 100, an adsorbent layer 200, a hydrophobic sieve plate 300, a filter membrane 400, a sealing cover 500 and a vacuum pump 600, wherein the hydrophobic sieve plate 300 is disposed in a bottom cavity 101 of the main body 100, the adsorbent layer 200 is disposed above the hydrophobic sieve plate 300, the filter membrane 400 is disposed below the hydrophobic sieve plate 300, the sealing cover 500 is disposed above the main body, and the bottom of the main body 100 extends outward to form an opening 102. The opening 102 is coupled to the hermetic container 700 to form a hermetic space, and the hermetic container 700 is connected to the vacuum pump 600 through a pipeline. The opening 102 may be designed in a "V" shape to facilitate the connection of the sample processing device to the sealed container 700. The sample processing device in the embodiment can be used for processing homogenized corn samples, homogenized spinach samples, homogenized apple samples, homogenized pakchoi samples and the like. The following is an example of the treatment of a homogenized corn sample.
Example 6
Corn samples were processed using the sample processing device of example 5 as an extraction tube.
In this example, the main body 100 of the sample extraction and separation apparatus is a polypropylene column tube having an inner diameter of 3cm, the hydrophobic sieve plate 300 is a polytetrafluoroethylene sieve plate having a thickness of 3mm and an aperture of 10 μm, the adsorbent layer 200 formed of 6g of anhydrous magnesium sulfate and 1.5g of sodium acetate is filled above the hydrophobic sieve plate 300, the filter membrane 400 formed of a nylon filter membrane having an aperture of 0.22 μm is disposed below the hydrophobic sieve plate 300, and the sealing cap 500 is disposed above the main body 100. The sealing cover 500 is integrally formed with the main body 100.
Adding 10g homogenized corn sample above adsorbent layer 200, adding 10ml extraction solution (acetonitrile and water ratio of 1:9-1:1), covering with sealing cover 500, and shaking and mixing. Finally, the sealing cap 500 is opened, the vacuum pump 600 is opened, and the pressure is adjusted to 0.06MPa, so that the sample-extracting solution penetrates through the hydrophobic sieve plate 300 and the filtering membrane 400, and enters the sealed container 700 through the opening 102.
Example 7
Corn samples were processed using the sample processing apparatus of example 5 as a purge tube.
In this example, the main body 100 of the sample separation apparatus is a polypropylene column having an inner diameter of 3cm, the hydrophobic sieve plate 300 is a polytetrafluoroethylene sieve plate having a thickness of 5mm and an aperture of 20 μm, the adsorbent layer 200 formed of 400mg PSA, 400mg C18 and 1200mg anhydrous sodium sulfate is filled above the hydrophobic sieve plate 300, the filtration membrane 400 formed of a polytetrafluoroethylene filtration membrane having an aperture of 0.22 μm is disposed below the hydrophobic sieve plate 300, and the sealing cover 500 is disposed above the main body 100. The sealing cover 500 is integrally formed with the main body 100.
8ml of the extraction solution collected in example 6 is added above the adsorbent layer 200, and then the mixture is mixed by shaking, and finally the vacuum pump 600 is turned on, and the pressure is adjusted to 0.06MPa, so that the solution after sample purification penetrates through the hydrophobic sieve plate 300 and the filter membrane 400, and enters the sealed container 700 through the opening 102.
Example 8
As shown in fig. 7, the present embodiment provides a sample processing apparatus, which includes a main body 100, an adsorbent layer 200, a hydrophobic sieve plate 300, a filter membrane 400, a sealing cover 500 and a booster 800, wherein the hydrophobic sieve plate 300 is disposed in a bottom cavity 101 of the main body 100, the adsorbent layer 200 is disposed above the hydrophobic sieve plate 300, the filter membrane 400 is disposed below the hydrophobic sieve plate 300, the sealing cover 500 is disposed above the main body, and the bottom of the main body 100 extends outward to form an opening 102. The booster 800 is coupled to the main body 100 to form a closed space, and the booster 800 is slidable along the inner wall of the main body 100 to form a positive pressure above the hydrophobic sieve plate 300 when the booster 800 slides downward. The sample processing device in the embodiment can be used for processing homogenized corn samples, homogenized spinach samples, homogenized apple samples, homogenized pakchoi samples and the like. The following will describe the treatment of homogenized pakchoi samples as an example.
In the embodiment, the adsorbent layer 200 consists of 4g of anhydrous magnesium sulfate, 1g of sodium chloride, 0.5g of disodium hydrogen citrate and 1g of sodium citrate; the hydrophobic sieve plate 300 is a polytetrafluoroethylene filter sieve plate with the thickness of 3mm and the aperture of 10 mu m; the filtration membrane 400 was a nylon filtration membrane having a pore size of 0.22. mu.m.
Adding 10g homogenized caulis et folium Brassicae Capitatae sample above adsorbent layer 200, adding 10ml extractive solution (acetonitrile and water ratio of 1:9-1:1), covering with sealing cover 500, and shaking and mixing. The sealing cap 500 is opened, the booster 800 is inserted into the cavity 101 of the main body 100, and a pressure of 0.06MPa is applied to allow the sample extraction solution to pass through the hydrophobic sieve 300 and the filtration membrane 400, and flow out through the opening 102 to be collected. In practice, booster 800 may replace seal cap 500.
The utility model discloses in the sample extraction separator that provides not only can be used for the extraction or the separation of homogenate material, also can be applied to the separation of macromolecular substance and micromolecular substance with it.
The utility model provides a sample extraction separator receives pressure differential's control, it can part pending sample effectively, avoids complicated operating procedure such as centrifugation, can realize collecting the oscillation of sample moreover and mix operation such as, the separation of sample in an organic whole, accomplishes in an operating system promptly, need not to mix operation such as oscillation and separation and go on respectively in the container of difference, and its more is suitable for the processing of a small amount of samples, has avoided the loss that causes the sample when changing the container.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalents and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. A pressure difference-controlled sample extraction and separation device, comprising: a main body and a hydrophobic sieve plate,
the main body comprises a cavity, and the hydrophobic sieve plate is arranged in the cavity and used for blocking objects above the sieve plate from falling;
an opening is formed in the bottom of the main body;
when the sample is extracted, the hydrophobic sieve plate is used for preventing the extraction solvent from passing through;
when the sample is separated, when a pressure difference exists between two sides of the hydrophobic sieve plate, the sample components needing to be separated can pass through the hydrophobic sieve plate;
the aperture of the hydrophobic sieve plate is 2-20 μm; the hydrophobic sieve plate is made of porous polytetrafluoroethylene.
2. The sample extraction separation device of claim 1 wherein a sealing cap is provided over the body for sealing the body.
3. The sample extraction separation apparatus of claim 1, wherein the hydrophobic sieve plate has a pore size of 2 μm, 5 μm, 8 μm, 10 μm, 12 μm, 15 μm, 18 μm, or 20 μm.
4. The sample extraction separation device of claim 1, wherein the hydrophobic screen has a thickness of 0.5mm to 5 mm.
5. The sample extraction separation device of any one of claims 1-4, further comprising: a filter membrane and/or an adsorbent layer, the filter membrane being disposed below the hydrophobic sieve plate; the adsorbent layer is arranged above the hydrophobic sieve plate.
6. The sample extraction separation apparatus as claimed in claim 5, wherein the pore size of the filtration membrane is 0.2 μm to 1 μm.
7. The sample extraction separation device as claimed in claim 5, wherein the material of the filtering membrane is selected from one or more of polypropylene, polyethersulfone, nylon, polyvinylidene fluoride and polytetrafluoroethylene.
8. The sample extraction and separation device of claim 5, wherein the adsorbent of the adsorbent layer is selected from one or more of anhydrous sodium sulfate, anhydrous magnesium sulfate, sodium acetate, sodium chloride, C18 bonded silica gel, graphitized carbon, N-propyl ethylenediamine bonded silica gel, and polystyrene divinyl benzene high polymer.
9. A sample processing device comprising a sample extraction and separation device according to any of claims 1 to 8 and a pressure device for providing a pressure differential to the sample extraction and separation device.
10. The sample processing device of claim 9, wherein the pressure differential is between 0.01MPa and 0.1 MPa.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109342162A (en) * | 2018-11-29 | 2019-02-15 | 天津博纳艾杰尔科技有限公司 | A kind of sample extraction separator and sample processing device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109342162A (en) * | 2018-11-29 | 2019-02-15 | 天津博纳艾杰尔科技有限公司 | A kind of sample extraction separator and sample processing device |
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