CN210146568U

CN210146568U - Negative pressure cleaning device

Info

Publication number: CN210146568U
Application number: CN201920884764.XU
Authority: CN
Inventors: 卿志荣; 郭新院
Original assignee: Jiangsu Ruibao Biotechnology Co Ltd
Current assignee: Jiangsu Ruibao Biotechnology Co Ltd
Priority date: 2019-06-12
Filing date: 2019-06-12
Publication date: 2020-03-17
Anticipated expiration: 2029-06-12

Abstract

The utility model provides a negative pressure cleaning device, which comprises a box body, a placing cover, an adsorption column and a negative pressure suction apparatus, wherein a suction cavity is formed inside the box body; the placing cover is covered on the box body, and at least one through placing hole is formed in the placing cover; an adsorption hole is formed in the adsorption column, an inlet and an outlet are formed at two ends of the adsorption hole respectively, the adsorption column is inserted into the placing hole, and the adsorption hole is communicated with the suction cavity; the negative pressure suction device is communicated with the suction cavity and is used for sucking liquid in the suction cavity. The negative pressure cleaning device is specially designed for a cleaning mode of negative pressure adsorption, can be suitable for cleaning macromolecular preparations, such as protein, nucleic acid, polysaccharide and other preparations, is simpler to operate, has lower cost, can adapt to adsorption columns of various specifications on the market, and can prevent cross contamination between adjacent adsorption columns.

Description

Negative pressure cleaning device

Technical Field

The utility model relates to a negative pressure belt cleaning device.

Background

Macromolecular preparations, such as protein, nucleic acid, polysaccharide, etc., require purification before use. For example, before the nucleic acid is subjected to an elution step in a purification process, the nucleic acid needs to be washed to remove impurities, thereby improving the purity of the nucleic acid. In the purification treatment process, after nucleic acid is cleaned, a cleaning agent (i.e., waste liquid) containing impurities needs to be sucked away to achieve the purpose of purifying nucleic acid. A common way of sucking away the washing agent is centrifugation, which is to separate the purified nucleic acid from the washing agent by centrifugal force. The cleaning agent is sucked away, a negative pressure suction mode can be considered, but an experimental device with good performance matched with the cleaning agent is not available on the market.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a negative pressure belt cleaning device reaches the purpose through negative pressure suction cleaner after can rinsing macromolecular preparations such as nucleic acid.

In order to achieve the above object, the utility model provides a following technical scheme:

the utility model provides a negative pressure cleaning device, which comprises a box body, a placing cover, an adsorption column and a negative pressure suction apparatus, wherein a suction cavity is formed inside the box body; the placing cover is covered on the box body, and at least one through placing hole is formed in the placing cover; an adsorption hole is formed in the adsorption column, an inlet and an outlet are formed at two ends of the adsorption hole respectively, the adsorption column is inserted into the placing hole, and the adsorption hole is communicated with the suction cavity; the negative pressure suction device is communicated with the suction cavity and is used for sucking liquid in the suction cavity.

In the technical scheme, the negative pressure aspirator continuously generates negative pressure to pump out waste liquid in the suction cavity; meanwhile, the interior of the adsorption column is kept at negative pressure, so that the cleaning agent can smoothly flow into the suction cavity.

Preferably, the box body is further provided with at least one flow guide hole, the flow guide hole is arranged corresponding to the adsorption hole, and the adsorption hole at the corresponding position is communicated with the suction cavity through the flow guide hole.

In this technical scheme, the water conservancy diversion hole plays the effect of absorbing the downthehole liquid suction chamber of absorption of corresponding position.

Preferably, one end of the adsorption column having the discharge port is located inside the guide hole.

In this technical scheme, be separated completely between the adjacent adsorption column, avoided the mutual contamination between the adjacent adsorption column to can place more adsorption columns in limited space, make negative pressure belt cleaning device's volume accomplish littleer, more conveniently carry and operate.

Preferably, the negative pressure aspirator comprises a liquid collecting bottle, an exhaust tube and a negative pressure terminal, wherein the liquid collecting bottle is provided with a liquid inlet communicated with the suction cavity; and two ends of the exhaust tube are respectively communicated with the liquid collecting bottle and the negative pressure terminal, and the negative pressure terminal is used for extracting gas in the liquid collecting bottle.

In this technical scheme, the gas in the collection liquid bottle is extracted to the negative pressure terminal, makes the interior production negative pressure of collection liquid bottle to collect the liquid bottle with the waste liquid in the suction chamber.

Preferably, the outlet of the suction cavity communicated with the liquid inlet of the liquid collecting bottle is positioned at the bottom of the suction cavity.

In this technical scheme, the export in suction chamber is located the bottom in suction chamber, is convenient for inhale the liquid trap with the waste liquid.

Preferably, the liquid inlet of the liquid collecting bottle and/or the air outlet of the liquid collecting bottle communicated with the air exhaust pipe are/is positioned at the upper part of the liquid collecting bottle.

In the technical scheme, the liquid inlet of the liquid collecting bottle is positioned at the upper part of the liquid collecting bottle, so that the waste liquid can conveniently flow into the liquid collecting bottle; the gas outlet of the liquid collecting bottle is positioned at the upper part of the liquid collecting bottle, so that the air can be conveniently pumped.

Preferably, the number of the placing holes is multiple, and the number of the adsorption columns is equal to or less than the number of the placing holes; the negative pressure cleaning device also comprises a plurality of plugs, each placing hole is inserted into one of the adsorption column and the plugs, and the plugs are in sealing fit with the inserted placing holes.

In the technical scheme, the number of the adsorption columns is determined by the number of the macromolecular preparations to be cleaned, and the plugs are placed in the vacant placing holes, so that the vacant placing holes cannot leak air, and the suction work of the negative pressure suction apparatus on the adsorption columns is influenced.

Preferably, the box body includes shell and intermediate lamella, the shell forms and has the open-ended chamber that holds, the intermediate lamella is located hold the intracavity, open-ended first surface is kept away from to the intermediate lamella with the internal surface that holds the chamber encloses becomes the suction chamber, be equipped with the water conservancy diversion hole on the intermediate lamella, the water conservancy diversion hole is followed open-ended first surface is kept away from to the intermediate lamella is close to open-ended second surface.

In this technical scheme, through the setting of shell and intermediate lamella, make the inside formation of box body hold the chamber to form on the box body and hold the water conservancy diversion hole that the chamber is linked together.

Preferably, the outer shell comprises an upper shell and a lower shell, the inner surface of the upper shell and the inner surface of the lower shell enclose the accommodating cavity, the inner surface of the lower shell is provided with a limiting table arranged around the circumferential direction, the limiting table is provided with a placing platform arranged along a radial plane and a limiting wall arranged around the circumferential direction, the first surface of the middle plate rests on the placing platform, and the outer circumferential surface of the middle plate is in contact with the limiting wall.

In this technical scheme, divide into casing, lower casing with the shell, the installation and the dismantlement of the box body of being convenient for wash, fix a position the intermediate lamella in the lower casing simultaneously, make the box body can the rapid Assembly, the equipment mistake can not appear.

Preferably, a first positioning ring protruding towards the lower shell is arranged on a contact surface of the upper shell and the lower shell, a second positioning ring protruding towards the upper shell is arranged on a contact surface of the lower shell and the upper shell, and the outer peripheral surface of the second positioning ring is matched with the inner peripheral surface of the first positioning ring.

In this technical scheme, through the cooperation of first holding ring, second holding ring, guaranteed the accuracy of the relative position between last casing, the lower casing, guaranteed the installation place on last casing cover and the relative position's of installation between the intermediate lamella on the casing down the accuracy to guaranteed the water conservancy diversion hole, placed the hole and can correspond the setting.

Preferably, the housing further comprises a sealing ring, and the sealing ring is arranged on a contact surface where the upper shell and the lower shell are contacted.

In this technical scheme, the contact surface of going up casing, casing contact down is equipped with the sealing washer, guarantees the leakproofness between casing, the casing down.

Preferably, the negative pressure cleaning device further comprises a sealing ring, the sealing ring is arranged between the placing cover and the box body, and the sealing ring is arranged around the placing hole.

In this technical scheme, through setting up the sealing ring, make place the lid and install on the box body to after the adsorption column is good, gas can only flow from the circulation direction along adsorption hole, water conservancy diversion hole, and can not follow other contact surfaces gas leakage, guaranteed the absorptive effect of negative pressure.

Preferably, a sealing groove surrounding the placing hole is formed in the surface, facing the placing cover, of the box body, and the sealing ring is placed in the sealing groove.

In the technical scheme, the sealing groove is used for positioning and placing the sealing ring, so that the sealing ring is convenient to install quickly, and the sealing effect is ensured.

Preferably, one end of the adsorption column having the inlet is positioned outside the placing hole, and one end of the adsorption column having the outlet is positioned inside the diversion hole.

Preferably, the placing cover comprises a mounting plate and at least one mounting sleeve, and the mounting plate is provided with at least one through mounting hole; the two ends of the mounting sleeve are provided with openings, the mounting sleeve is inserted into the mounting hole, and the placing hole is formed in the inner circumferential surface of the mounting sleeve.

In this technical scheme, the installation cover is used for fixed adsorption column.

Preferably, the mounting sleeve is made of an elastic material.

In this technical scheme, the installation cover that uses elastic material to make, the change of the specification of adaptable adsorption column makes the installation cover remain the state of laminating with the adsorption column all the time.

On the basis of the common knowledge in the field, the above preferred conditions can be combined at will to obtain the preferred embodiments of the present invention.

The utility model discloses an actively advance the effect and lie in:

this negative pressure belt cleaning device designs to the adsorbed cleaning methods of negative pressure specially, and applicable in the washing of macromolecule preparation, for example the washing of preparations such as protein, nucleic acid, polysaccharide, and the centrifugal cleaning methods among the prior art, the operation is simpler, and the cost is lower, changes the realization automation.

Drawings

Fig. 1 is a schematic structural view of the negative pressure cleaning device of the present invention.

Fig. 2 is a schematic structural view of the negative pressure cleaning apparatus shown in fig. 1, except for the negative pressure aspirator.

Fig. 3 is an exploded view of the negative pressure cleaning apparatus shown in fig. 2.

Fig. 4 is a front view of the negative pressure cleaning apparatus shown in fig. 2.

Fig. 5 is a schematic view of the negative pressure cleaning apparatus shown in fig. 4 in the direction of a-a.

Fig. 6 is a partially enlarged view of the negative pressure cleaning apparatus shown in fig. 5.

Description of the reference numerals

A box body 1; suction chamber 11, outlet 111; a diversion hole 12; the positioning device comprises a shell 13, an opening 131, a containing cavity 132, an upper shell 133, a lower shell 134, a limiting table 135, a placing platform 136, a limiting wall 137, a sealing ring 138, a first positioning ring 139 and a second positioning ring 1310; the intermediate plate 14, the first surface 141, the second surface 142; a seal groove 15;

placing the cover 2; a placement hole 21; mounting plate 22, mounting hole 221; a mounting sleeve 23;

an adsorption column 3; an adsorption hole 31, an inlet port 32, an outlet port 33;

a negative pressure aspirator 4; a liquid collecting bottle 41, a liquid inlet 411 and a gas outlet 412; an exhaust tube 42; a negative pressure terminal 43; the communicating pipe 44;

and a seal ring 5.

Detailed Description

The present invention will be further described with reference to the following embodiments and drawings, and more details will be set forth in the following description in order to provide a thorough understanding of the present invention, but it is obvious that the present invention can be implemented in various other ways different from those described herein, and those skilled in the art can make similar generalizations and deductions according to the actual application without departing from the spirit of the present invention, and therefore, the scope of the present invention should not be limited by the contents of the embodiments.

Fig. 1 to 6 show an embodiment of the negative pressure cleaning device of the present invention, which includes a box body 1, a placing cover 2, an adsorption column 3 and a negative pressure suction apparatus 4, wherein a suction chamber 11 is formed inside the box body 1, the placing cover 2 covers the box body 1, a plurality of through placing holes 21 are formed on the placing cover 2, an adsorption hole 31 is formed inside the adsorption column 3, an inlet 32 and an outlet 33 are respectively formed at two ends of the adsorption hole 31, the adsorption column 3 is inserted into the placing hole 21, and the adsorption hole 31 is communicated with the suction chamber 11; the negative pressure aspirator 4 is communicated with the suction cavity 11, and the negative pressure aspirator 4 is used for sucking the liquid in the suction cavity 11.

When the macromolecular preparation (such as a nucleic acid preparation) needs to be cleaned, the macromolecular preparation is placed into the adsorption column 3, a cleaning agent is injected into the adsorption column 3, and the cleaning agent is used for cleaning the macromolecular preparation. The impurities flow out from the discharge port 33 of the adsorption column 3 along with the cleaning agent and then flow into the suction cavity 11 through the flow guide hole 12; and macromolecules such as nucleic acid are adsorbed on the adsorption column 3. The negative pressure aspirator 4 continuously generates negative pressure to extract the waste liquid in the suction cavity 11; meanwhile, the interior of the adsorption column 3 is kept at negative pressure, so that the cleaning agent can smoothly flow into the suction cavity 11.

The box body 1 is also provided with a plurality of diversion holes 12, the diversion holes 12 are arranged corresponding to the adsorption holes 31, and the corresponding adsorption holes 31 of the diversion holes 12 are communicated with the suction cavity 11. The guide hole 12 plays a role of sucking the liquid in the adsorption hole 31 at the corresponding position into the suction chamber 11. The number of the flow guide holes 12 may be one or more, and the number of the placing holes 21 may also be one or more. In order to dispose the guide holes 12 corresponding to the adsorption holes 31, the guide holes 12 are disposed in the same number as and correspond to the placement holes 21. The number of the diversion holes 12 and the placement holes 21 can be designed according to the maximum number of preparations to be treated simultaneously.

And, the number of the adsorption columns 3 is equal to or smaller than the number of the placing holes 21. The number of columns 3 to be used depends on the number of macromolecular preparations to be washed. When the number of the adsorption columns 3 to be used is the same as the number of the placement holes 21, one adsorption column 3 is inserted into each placement hole 21. When the number of the adsorption columns 3 to be used is smaller than the number of the placing holes 21, a plug is put in the empty placing hole 21, and the plug is in sealing engagement with the inserted placing hole 21. Thus, the empty placing hole 21 can not be air-leaked, and the suction work of the negative pressure suction apparatus 4 on the adsorption column 3 can not be influenced.

For example, when the number of the diversion holes 12 and the placement holes 21 is 48, the number of the adsorption columns 3 may be 1 to 48. When the number of the adsorption columns 3 is less than 48, the plugs are placed in the vacant placing holes 21. For another example, when the number of the flow guide holes 12 and the placement holes 21 is 96, the number of the adsorption columns 3 may be 1 to 96. When the number of the adsorption columns 3 is less than 96, the plugs are placed in the vacant placing holes 21.

As shown in fig. 1, the negative pressure aspirator 4 comprises a liquid collecting bottle 41, an air suction pipe 42 and a negative pressure terminal 43, wherein the liquid collecting bottle 41 is provided with a liquid inlet 411, and the liquid inlet 411 is communicated with the suction cavity 11; the two ends of the air exhaust tube 42 are respectively communicated with the liquid collecting bottle 41 and the negative pressure terminal 43, and the negative pressure terminal 43 is used for exhausting the gas in the liquid collecting bottle 41. The negative pressure terminal 43 draws the gas in the liquid collecting bottle 41 to generate a negative pressure in the liquid collecting bottle 41, thereby collecting the waste liquid in the suction chamber 11 into the liquid collecting bottle 41.

As shown in FIG. 1, the negative pressure aspirator 4 further comprises a communication pipe 44, and the communication pipe 44 communicates the liquid inlet 411 of the liquid collection bottle 41 with the outlet 111 of the suction chamber 11. To facilitate pipetting, the outlet 111 of the aspiration chamber 11 is located at the bottom of the aspiration chamber 11. In addition, for the convenience of air extraction, an air outlet 412, which communicates the liquid collecting bottle 41 and the air extraction pipe 42, is located at the upper part of the liquid collecting bottle 41. The liquid inlet 411 of the liquid collecting bottle 41 is also located at the upper part of the liquid collecting bottle 41, so that the waste liquid can flow into the liquid collecting bottle 41 conveniently.

As shown in fig. 3, 5 and 6, the box body 1 includes a housing 13 and an intermediate plate 14, the housing 13 forms an accommodating cavity 132 with an opening 131, the intermediate plate 14 is located in the accommodating cavity 132, a first surface 141 of the intermediate plate 14 far from the opening 131 and an inner surface of the accommodating cavity 132 enclose a suction cavity 11, a diversion hole 12 is provided on the intermediate plate 14, and the diversion hole 12 extends from the first surface 141 of the intermediate plate 14 far from the opening 131 to a second surface 142 of the intermediate plate 14 close to the opening 131.

As shown in fig. 5 to 6, in order to facilitate mounting and dismounting and cleaning, the housing 13 includes an upper casing 133 and a lower casing 134, an inner surface of the upper casing 133 and an inner surface of the lower casing 134 enclose a receiving chamber 132, an inner surface of the lower casing 134 is provided with a stopper 135 disposed around a circumferential direction, the stopper 135 has a placing platform 136 disposed along a radial plane and a stopper wall 137 disposed around the circumferential direction, a first surface 141 of the intermediate plate 14 rests on the placing platform 136, and an outer circumferential surface of the intermediate plate 14 is in contact with the stopper wall 137.

During the installation process, the middle plate 14 is placed in the lower shell 134, the first surface 141 of the middle plate 14 is placed on the placing platform 136, and the outer peripheral surface of the middle plate 14 is in contact with the limiting wall 137, so that the position of the middle plate 14 in the lower shell 134 is fixed; the upper case 133 is mounted on the lower case 134.

In order to ensure the accuracy of the relative position between the upper and

lower cases

133 and 134, the contact surface of the upper and

lower cases

133 and 134 has a first positioning ring 139 protruding toward the lower case 134, and the contact surface of the lower case 134 and the upper case 133 has a second positioning ring 1310 protruding toward the upper case 133.

When the upper housing 133 is mounted on the lower housing 134, the second positioning ring 1310 is within the first positioning ring 139, and the outer peripheral surface of the second positioning ring 1310 mates with the inner peripheral surface of the first positioning ring 139. Through the cooperation of the first positioning ring 139 and the second positioning ring 1310, the accuracy of the relative position between the upper casing 133 and the lower casing 134 is ensured, so that the accuracy of the relative position between the placing cover 2 mounted on the upper casing 133 and the middle plate 14 mounted on the lower casing 134 is ensured, and the placing hole 21 and the diversion hole 12 can be correspondingly arranged.

In order to ensure the sealing property between the upper casing 133 and the lower casing 134, a gasket 138 is provided on the contact surface between the upper casing 133 and the lower casing 134.

The structure of the box body 1 is arranged, so that the box body 1 is integrally easy to disassemble, and is convenient to clean after an experiment. Meanwhile, when the device is installed, the device can be quickly combined, and assembly errors can not occur.

In order to guarantee the tightness of the contact surface between the placing cover 2 and the box body 1, the negative pressure cleaning device further comprises a sealing ring 5, the sealing ring 5 is arranged between the placing cover 2 and the box body 1, and the sealing ring 5 is arranged around the placing hole 21. For placing the sealing ring 5, the surface of the case 1 facing the placing cover 2 is provided with a sealing groove 15 arranged around the placing hole 21, in which sealing groove 15 the sealing ring 5 is placed.

Through above-mentioned seal structure, make place lid 2 and install on box body 1 to after having installed adsorption column 3, gaseous circulation direction that can only follow along adsorption hole 31, water conservancy diversion hole 12 flows, and can not follow other contact surfaces gas leakage, guaranteed the absorptive effect of negative pressure.

As shown in fig. 6, in order to avoid contamination, the end of the adsorption column 3 having the inlet port 32 is located outside the placement hole 21, and the end of the adsorption column 3 having the outlet port 33 is located inside the guide hole 12. Thus, the waste liquid flowing out of the adsorption column 3 firstly enters the diversion holes 12 and then flows into the suction cavity 11. The adjacent adsorption columns 3 are completely separated, so that mutual pollution between the adjacent adsorption columns 3 is avoided, more adsorption columns 3 can be placed in a limited space, the volume of the negative pressure cleaning device can be smaller, and the negative pressure cleaning device is more convenient to carry and operate.

As shown in fig. 5 to 6, the placing cover 2 includes a mounting plate 22 and a plurality of mounting sleeves 23, and a plurality of mounting holes 221 are formed through the mounting plate 22; both ends of the mounting sleeve 23 are opened, the mounting sleeve 23 is inserted into the mounting hole 221, and the inner peripheral surface of the mounting sleeve 23 forms the placing hole 21. The mounting sleeve 23 is used for fixing the adsorption column 3. The mounting sleeve 23 is preferably made of an elastomeric material, such as silicone or the like. The mounting sleeve 23 made of elastic material can adapt to the change of the shape of the adsorption column 3, so that the mounting sleeve 23 is always attached to the adsorption column 3.

The assembling steps of the negative pressure cleaning device are as follows:

1) the pre-cleaning work of the upper shell 133, the lower shell 134, the middle plate 14, the mounting plate 22, the mounting sleeve 23, the adsorption column 3 and other parts is completed;

2) placing the intermediate plate 14 in the lower case 134 with the lower surface of the intermediate plate 14 resting on the position restricting table 135, the first surface 141 of the intermediate plate 14 resting on the placement platform 136, and the outer peripheral surface of the intermediate plate 14 in contact with the position restricting wall 137;

3) the upper shell 133 is installed on the lower shell 134, the second positioning ring 1310 is arranged in the first positioning ring 139, the outer peripheral surface of the second positioning ring 1310 is matched with the inner peripheral surface of the first positioning ring 139, and the upper shell 133, the lower shell 134 and the middle plate 14 form a box body 1;

4) the mounting sleeve 23 is mounted in the mounting hole 221 of the mounting plate 22 to constitute the set cover 2.

The negative pressure cleaning method using the negative pressure cleaning device comprises the following steps:

① installing the adsorption column 3 into the installation sleeve 23, placing a plug into the empty placing hole 21, the plug sealing matching with the inserted placing hole 21;

② placing the sealing ring 5 in the sealing groove 15 of the box body 1, then placing the placing cover 2 on the sealing ring 5, the adsorption hole 31 of the adsorption column 3 is communicated with the suction cavity 11;

③ dropping the macromolecule to be cleaned into the adsorption hole 31 to make the macromolecule adsorbed on the inner circumferential surface of the adsorption hole 31;

④ the cleaning agent is injected into the adsorption hole 31 to clean the macromolecule adsorbed in the adsorption hole 31, and simultaneously the vacuum extractor 4 is opened to form negative pressure in the suction cavity 11 and the adsorption hole 31, so that the cleaning agent and the impurities of the macromolecule to be washed out flow into the vacuum extractor 4 from the suction cavity 11.

The negative pressure cleaning device is suitable for cleaning macromolecular preparations, such as preparations of protein, nucleic acid, polysaccharide and the like. Besides being suitable for cleaning macromolecular preparations, the cleaning process by using a negative pressure adsorption mode can be suitable for the negative pressure cleaning device. Through using above-mentioned negative pressure belt cleaning device, utilize the negative pressure to wash macromolecule preparation, compare the centrifugal washing mode among the prior art, the operation is simpler, and the cost is lower.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the present invention, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Any modification, equivalent changes and modifications of the above embodiments according to the technical spirit of the present invention, which do not depart from the technical scope of the present invention, fall within the protection scope defined by the claims of the present invention.

Claims

1. A negative pressure cleaning device, comprising:

a box body, wherein a suction cavity is formed inside the box body;

the placing cover is covered on the box body, and at least one through placing hole is formed in the placing cover;

at least one adsorption column, wherein an adsorption hole is formed inside the adsorption column, an inlet and an outlet are respectively formed at two ends of the adsorption hole, the adsorption column is inserted into the placing hole, and the adsorption hole is communicated with the suction cavity;

the negative pressure suction device is communicated with the suction cavity and is used for sucking liquid in the suction cavity.

2. The negative-pressure cleaning device according to claim 1, wherein the box body is further provided with at least one flow guide hole, the flow guide hole is arranged corresponding to the adsorption hole, and the flow guide hole connects the adsorption hole at the corresponding position with the suction cavity.

3. The negative pressure cleaning apparatus of claim 2, wherein: one end of the adsorption column with the discharge port is positioned in the diversion hole.

4. The negative pressure cleaning device of claim 1, wherein the negative pressure aspirator comprises:

the liquid collecting bottle is provided with a liquid inlet which is communicated with the suction cavity;

the two ends of the exhaust pipe are respectively communicated with the liquid collecting bottle and the negative pressure terminal, and the negative pressure terminal is used for extracting gas in the liquid collecting bottle.

5. The negative pressure cleaning device as claimed in claim 4, wherein the outlet of the suction chamber communicating with the liquid inlet of the liquid collecting bottle is positioned at the bottom of the suction chamber.

6. The negative-pressure cleaning device for cleaning the sewage tank is characterized in that the liquid inlet of the liquid collecting bottle and/or the air outlet of the liquid collecting bottle, which is communicated with the air suction pipe, are/is positioned at the upper part of the liquid collecting bottle.

7. The negative-pressure cleaning device according to claim 1, wherein the placement holes are plural in number, and the number of the adsorption columns is equal to or smaller than the number of the placement holes;

the negative pressure cleaning device also comprises a plurality of plugs, each placing hole is inserted into one of the adsorption column and the plugs, and the plugs are in sealing fit with the inserted placing holes.

8. The negative pressure cleaning apparatus of claim 1, wherein: the box body includes shell and intermediate lamella, the shell forms and has the open-ended chamber that holds, the intermediate lamella is located hold the intracavity, the intermediate lamella keep away from open-ended first surface with the internal surface that holds the chamber encloses into the suction chamber, be equipped with the water conservancy diversion hole on the intermediate lamella, the water conservancy diversion hole is followed the intermediate lamella is kept away from open-ended first surface and is extended to the intermediate lamella is close to open-ended second surface.

9. The negative pressure cleaning device of claim 8, wherein: the shell comprises an upper shell and a lower shell, the inner surface of the upper shell and the inner surface of the lower shell are enclosed to form the accommodating cavity, the inner surface of the lower shell is provided with a limiting table arranged around the circumferential direction, the limiting table is provided with a placing platform arranged along a radial plane and a limiting wall arranged around the circumferential direction, the first surface of the middle plate is placed on the placing platform, and the peripheral surface of the middle plate is in contact with the limiting wall.

10. The negative pressure cleaning device of claim 9, wherein: the contact surface of the upper shell and the lower shell is provided with a first positioning ring protruding towards the direction of the lower shell, the contact surface of the lower shell and the upper shell is provided with a second positioning ring protruding towards the direction of the upper shell, and the outer peripheral surface of the second positioning ring is matched with the inner peripheral surface of the first positioning ring.

11. The negative pressure cleaning device of claim 9, wherein: the shell further comprises a sealing ring, and the sealing ring is arranged on a contact surface where the upper shell and the lower shell are in contact.

12. The negative pressure cleaning apparatus of claim 1, wherein: the negative pressure cleaning device further comprises a sealing ring, the sealing ring is arranged between the placing cover and the box body, and the sealing ring surrounds the placing hole.

13. The negative pressure cleaning device of claim 12, wherein: the surface of the box body facing the placing cover is provided with a sealing groove surrounding the placing hole, and the sealing ring is placed in the sealing groove.

14. The negative pressure cleaning apparatus of claim 1, wherein the placement cap comprises:

the mounting plate is provided with at least one through mounting hole;

the two ends of the mounting sleeve are provided with openings, the mounting sleeve is inserted into the mounting hole, and the placing hole is formed in the inner circumferential surface of the mounting sleeve.

15. The negative pressure cleaning apparatus of claim 14, wherein: the mounting sleeve is made of an elastic material.