CN210134124U - Novel cell recovery device - Google Patents
Novel cell recovery device Download PDFInfo
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- CN210134124U CN210134124U CN201920850941.2U CN201920850941U CN210134124U CN 210134124 U CN210134124 U CN 210134124U CN 201920850941 U CN201920850941 U CN 201920850941U CN 210134124 U CN210134124 U CN 210134124U
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- spring
- basket
- recovery
- resuscitation
- freezing
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Abstract
The utility model relates to a novel cell is come back to life or consciousness device, manage the recovery basket including a jar body, cover and freezing, jar body upper portion is sealed to be provided with the cover, the cover middle part is equipped with a rotation axis through the coaxial hinge that runs through of axle sleeve, the handle is equipped with admittedly on the rotation axis, the coaxial hinge of rotation axis lower extreme is equipped with freezing and manages the recovery basket, installs the spring barrel on the rotation axis between handle and freezing recovery tube basket, spring barrel coaxial arrangement is at the upper surface of cover, be provided with the spring clockwork spring in the spring barrel, the spring clockwork spring outer end is adorned admittedly on the inside wall fixed end of spring barrel, the inner of spring clockwork spring is adorned admittedly on the rotation axis, and the stress of spring clockwork spring drives freezing and manages recovery basket at jar internal rotation. The utility model discloses simple structure, keep warm effectual, constancy of temperature, melt efficiency fast and labour saving and time saving.
Description
Technical Field
The utility model belongs to the technical field of cell recovery, a cell recovery device technique is related to, especially a novel cell recovery device.
Background
Cell recovery means that the freezing tube is taken out from a liquid nitrogen container and is directly immersed in warm water at 37 ℃ and shaken to be melted as soon as possible without delay. However, in the prior art, an additional thermometer needs to be searched for testing the water temperature, the operation is troublesome, and if the water taking place is far away from the liquid nitrogen chamber or the water temperature is reduced in the cell thawing process, the stability of the water temperature is difficult to ensure, which indirectly affects the survival rate and the state of the cells. In addition, the cryopreservation tube is light and is not beneficial to uniform heating of cells when being placed into water to float, the state of the cells can be influenced, and the frozen cell tube is usually required to be manually shaken to rapidly melt cell strains, so that the workload is increased, and experimenters are easily injured after the cell tube is exploded; as the cell recovery amount is mostly more than 100 cell tubes or even more, the cell recovery work can be finished by at least two persons working for one day in actual operation, the time consumption is long, and the experiment progress is influenced; because the manual shaking force is uneven, the cell recovery time is different, and the ice crystals in the cells cause secondary damage to the cells due to long-time recovery, so that the subsequent experimental results are influenced.
The following patent documents relevant to the present application are found by search, and specific disclosures are as follows:
1. a rotary type frozen cell recovery device (CN208562407U) comprises a pot body, a top cover, a self-rotating mechanism arranged in the pot body and a circulating water device arranged on the left side of the pot body; the pot body comprises an outer shell, an inner shell and a heat insulation layer arranged between the outer shell and the inner shell, a baffle is transversely arranged at the lower part in the pot body, the left end of the baffle is hermetically connected with the pot body, the right end of the baffle is not in contact with the pot body, and an electric heating wire is arranged below the baffle; spin mechanism comprises an annular gear track, hook spare, driving motor and pipe support, the annular gear track passes through hook spare is hung and is established on the pot body, driving motor's output shaft lower extreme passes the annular gear track is connected the pipe support, the pipe support by driving motor drive follows in the rotation the annular gear track removes.
2. A cell recovery device (CN208562396U) with water bath circulation comprises a pot body, a top cover, a movable pipe frame arranged in the pot body and a water circulation device arranged on the left side of the pot body; the pan body comprises an outer shell, an inner shell and a heat insulation layer arranged between the outer shell and the inner shell, a baffle is transversely arranged at the lower part in the pan body, the left end of the baffle is hermetically connected with the pan body, the right end of the baffle is not in contact with the pan body, and an electric heating wire is arranged below the baffle; the movable pipe frame consists of a pair of slide rails horizontally arranged on the edge of the top of the pot body and a pipe plate slidably arranged on the slide rails, and the pipe plate is connected with a driving rod of a driving cylinder; and the circulating water device comprises a drain pipe communicated with the bottom of the pot body, a water inlet pipe communicated with the upper part of the pot body and a water pump connected with the drain pipe and the water inlet pipe.
3. Portable cell recovery box of keeping in (CN206666546U), comprises a box body, lid and memory box, a box body side-mounting drain pipe, a water for getting rid of in the box body, the sealed lid of stamping in box body upper portion, the lid is made for insulation material, the interval sets up a memory box in the box body, the memory box also is made for insulation material, memory box both sides lower part system has a plurality of through-holes, a memory box for water business turn over, in the upper surface equipartition through-hole of memory box, the pipe is deposited in freezing of through-hole embedded dress, the system has a through-hole on the lid, coaxial insertion thermometer in the through-hole, install the handle on the lid.
Through the contrast of technical characteristics, above-mentioned patent literature of disclosing with the utility model discloses a technical structure is inequality, can not influence the creativity and the novelty of the utility model application.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the weak point of prior art, provide a simple structure, keep warm effectual, constancy of temperature, melt efficient and labour saving and time saving's novel cell recovery device.
The utility model provides a its technical problem take following technical scheme to realize:
a novel cell recovery device comprises a tank body, a tank cover and a cryopreservation tube recovery basket, wherein a heat preservation layer is filled in the middle of the side wall of the tank body, a drainage tube is installed on one side of the tank body, the tank cover is arranged on the upper portion of the tank body in a sealing mode, a rotating shaft is installed in the middle of the tank cover through a shaft sleeve in a coaxial penetrating and hinging mode, a handle is fixedly installed at the upper end of the rotating shaft, the cryopreservation tube recovery basket is installed at the lower end of the rotating shaft in a coaxial hinging mode, a spring box is installed on the rotating shaft between the handle and the cryopreservation tube recovery basket and is coaxially installed on the upper surface of the tank cover, a spring is arranged in the spring box, the outer end of the spring is fixedly installed on the fixed end of the inner side wall of the spring box, the inner end of the spring is fixedly installed;
the utility model discloses a recovery basket, including resuscitation basket body and fixed picture peg, the freezing pipe resuscitation basket of depositing includes resuscitation basket body and fixed picture peg, this internal lower part of resuscitation basket is provided with fixed picture peg, resuscitation basket body upper surface equipartition interval system has a plurality of circular freezing pipe recess of depositing and deposits the hole, freezing pipe recess of depositing downthehole inlay the dress and freeze and deposit the pipe, it still makes to freeze and deposits a tub draw-in groove to correspond to freeze on depositing the fixed picture peg that the pipe recess was deposited the hole position, the side from last equipartition interval system of resuscitation basket body has a plurality of inlet openings.
And the surface of the tank cover is also provided with a thermometer fixing port, a thermometer is coaxially inserted into the thermometer fixing port, and the thermometer is contacted with the water surface in the tank body.
And a lifting handle is arranged on the tank body.
The utility model has the advantages that:
1. the utility model discloses simple structure, design benefit utilizes the clockwork spring principle for freezing and depositing tub recovery basket at jar internal rotation, effectively ensure to freeze and deposit intraductal cell and be heated and evenly melt with higher speed, and each freezes and deposits intraductal cell and melt rate unanimous basically, guarantee the survival rate of cell, make cell recovery operation convenient, swift, can once only accomplish the recovery operation of freezing and depositing the cell in a plurality of cells freezes and deposit, alleviate staff's work burden, improved work efficiency.
2. The utility model discloses when doing cell resuscitation and melting, with the cover housing on the jar body, and the experimenter can keep the certain distance with the jar body, effectively reduces the probability of cryopreserving pipe explosion injury experimenter, ensures experimenter safety.
3. The utility model discloses jar internal detachable thermometer conveniently measures the temperature, makes things convenient for jar body cleanness.
4. The utility model discloses a cell cryopreserving pipe can be rotatory in 37 ℃ water bath, and the cell thawing speed is fast, efficient, and through cryopreserving a tub draw-in groove of cryopreserving tub recovery basket with cryopreserve a tub recess and deposit the fixed pipe of cryopreserving of hole cooperation, stability is strong.
5. The utility model discloses an add constant temperature material in tank side wall middle part and realized the waters temperature control when cell resuscitates, provide required constant temperature waters for cell resuscitating, utilize the utility model discloses when carrying out cell resuscitating, can improve the security in the operation process, ensure cell resuscitating efficiency and cell stability, utilize the utility model discloses a many batches of cells of resuscitating simultaneously temporary storage device can resuscitate simultaneously, provide necessary experimental apparatus for experiment and scientific research cell resuscitating.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is the structural schematic diagram of the freezing storage pipe clamping groove of the utility model.
Detailed Description
The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.
A novel cell recovery device comprises a tank body 8, a tank cover 6 and a freezing tube recovery basket 9, wherein a heat preservation layer 13 is filled in the middle of the side wall of the tank body, a water drainage pipe 14 is installed on one side of the tank body and used for draining water in the tank body, the tank cover is arranged on the upper portion of the tank body in a sealing manner, a rotating shaft 4 is installed in the middle of the tank cover through a shaft sleeve coaxial hinge, the rotating shaft vertically penetrates through the tank cover, the freezing tube recovery basket is installed at the lower end of the rotating shaft in the tank body through a connecting piece 5 coaxial hinge, the freezing tube recovery basket is coaxially arranged in the tank body and is not in contact with the bottom of the tank body, a handle 1 is fixedly installed at the top end of the rotating shaft outside the tank body, a spring is installed on the rotating shaft between the handle and a freezing recovery pipe frame, a spring box 3 is arranged on the outer side of the spring box and is coaxially installed, the inner end of the spring is fixedly arranged on the rotating shaft, the releasing of the internal stress of the spring drives the rotating shaft to rotate and simultaneously drives the recovery basket of the cryopreservation tube to rotate in the tank body, so that cells in the cryopreservation tube are heated uniformly and are melted at an accelerated speed.
The upper surface of the tank cover is also provided with a thermometer fixing port, a thermometer 7 is coaxially inserted into the thermometer fixing port, the thermometer extends into the tank body, and the thermometer is contacted with the water surface in the tank body.
The tube recovery basket that freezes deposits includes recovery basket body and fixed picture peg 12, the structure of recovery basket body is cylindricly, the side from last equipartition interval system of recovery basket body has a plurality of inlet openings 10 for recovery basket body is soaked water, this internal lower part of recovery basket is provided with fixed picture peg, recovery basket body upper surface equipartition interval system has a plurality of circular freezing to deposit a tub recess and deposits the hole, it freezes to deposit the pipe recess and deposits downthehole the dress and freezes and deposit tub 11, still has to freeze to deposit tub slot 15 on the fixed picture peg of hole position corresponding to freezing to deposit tub recess, and this freezing is deposited tub slot and can be fixed with freezing the tub bottom of portion, prevents to freeze to deposit the pipe and is depositing downthehole rotation and avoid freezing the buoyancy of depositing tub water and break away from recovery basket body with it in freezing.
The utility model discloses in the implementation, for remove the convenience, can install handle 2 on the jar body.
The utility model discloses an implementation process does:
the utility model discloses a heat preservation heat-resisting material is added to the intermediate level of jar side wall, make the internal temperature of jar keep required constant temperature, open the cover, will inlay the freezing pipe recovery basket coaxial arrangement who has freezing pipe at the rotation axis lower extreme, then will freeze the pipe recovery basket and stretch into the jar internal, immerse in the warm water, and the sealed cover is good cover, place the thermometer in the thermometer fixed orifices, the thermometer contacts the internal surface of jar, monitor the change of the internal temperature of jar, water in this moment jar gets into in the basket fast through freezing pipe recovery basket's inlet opening, then anticlockwise rotation handle, the extension spring that spirals in the barrel at this moment is taut, because the spring all has elastic deformation, loosen the handle, the spring resets, drive freezing pipe recovery basket internal rotation in jar simultaneously, many times the rotation handle, thereby realize that the cell in freezing pipe is heated evenly melts with higher speed, after cell recovery process finishes, through the internal surplus water of drain pipe scrubbing jar, in order to make the water of freezing in the pipe recovery basket of depositing can remove cleanly, the twist grip is rotatory through clockwork spring principle and is frozen and deposit pipe recovery basket, freezes to deposit pipe recovery basket because rotatory centrifugation, throws away the water that does not flow out in the basket and freezes and deposit pipe recovery basket, opens the cover, takes off and freezes and deposit pipe recovery basket, takes out the pipe that freezes that needs to resuscitate, accomplishes cell recovery operation.
The utility model discloses simple structure, design benefit utilizes the clockwork spring principle for freezing and depositing tub recovery basket at jar internal rotation, effectively ensure to freeze and deposit intraductal cell and be heated and evenly melt with higher speed, and each freezes and deposits intraductal cell and melt rate unanimous basically, guarantee the survival rate of cell, make cell recovery operation convenient, swift, can once only accomplish the recovery operation of freezing and depositing the cell in a plurality of cells freezes and deposit, alleviate staff's work burden, improved work efficiency.
The utility model discloses when doing cell resuscitation and melting, with the cover housing on the jar body, and the experimenter can keep the certain distance with the jar body, effectively reduces the probability of cryopreserving pipe explosion injury experimenter, ensures experimenter safety.
The utility model discloses a jar body embeds detachable thermometer makes things convenient for the measurement temperature, makes things convenient for jar body cleanness.
The utility model discloses a cell cryopreserving pipe can be rotatory in 37 ℃ water bath, and the cell thawing speed is fast, efficient, and through cryopreserving a tub draw-in groove of cryopreserving tub recovery basket with cryopreserve a tub recess and deposit the fixed pipe of cryopreserving of hole cooperation, stability is strong.
The utility model discloses an add constant temperature material in tank side wall middle part and realized the waters temperature control when cell resuscitates, provide required constant temperature waters for cell resuscitating, utilize the utility model discloses when carrying out cell resuscitating, can improve the security in the operation process, ensure cell resuscitating efficiency and cell stability, utilize the utility model discloses a many batches of cells of resuscitating simultaneously temporary storage device can resuscitate simultaneously, provide necessary experimental apparatus for experiment and scientific research cell resuscitating.
Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.
Claims (3)
1. The utility model provides a novel cell recovery device, includes jar body, cover and freezes deposits tub recovery basket, the heat preservation is filled at jar side wall middle part, jar body side-mounting drain pipe, jar body upper portion is sealed to be provided with cover, its characterized in that: the middle part of the tank cover is coaxially and penetratingly hinged with a rotating shaft through a shaft sleeve, the upper end of the rotating shaft is fixedly provided with a handle, the lower end of the rotating shaft is coaxially hinged with a cryopreservation tube recovery basket, a spring barrel is arranged on the rotating shaft between the handle and the cryopreservation tube recovery basket, the spring barrel is coaxially arranged on the upper surface of the tank cover, a spring is arranged in the spring barrel, the outer end of the spring is fixedly arranged on the fixed end of the inner side wall of the spring barrel, the inner end of the spring is fixedly arranged on the rotating shaft, the stress of the spring drives the cryopreservation tube recovery basket to rotate in the tank body, and cells in the cryopreservation tube are heated uniformly and are molten;
the utility model discloses a recovery basket, including resuscitation basket body and fixed picture peg, the freezing pipe resuscitation basket of depositing includes resuscitation basket body and fixed picture peg, this internal lower part of resuscitation basket is provided with fixed picture peg, resuscitation basket body upper surface equipartition interval system has a plurality of circular freezing pipe recess of depositing and deposits the hole, freezing pipe recess of depositing downthehole inlay the dress and freeze and deposit the pipe, it still makes to freeze and deposits a tub draw-in groove to correspond to freeze on depositing the fixed picture peg that the pipe recess was deposited the hole position, the side from last equipartition interval system of resuscitation basket body has a plurality of inlet openings.
2. The novel cell resuscitation device according to claim 1, wherein: the surface of the tank cover is also provided with a thermometer fixing port, a thermometer is coaxially inserted into the thermometer fixing port, and the thermometer is contacted with the water surface in the tank body.
3. The novel cell resuscitation device according to claim 1, wherein: and a lifting handle is arranged on the tank body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920850941.2U CN210134124U (en) | 2019-06-06 | 2019-06-06 | Novel cell recovery device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920850941.2U CN210134124U (en) | 2019-06-06 | 2019-06-06 | Novel cell recovery device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210134124U true CN210134124U (en) | 2020-03-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920850941.2U Active CN210134124U (en) | 2019-06-06 | 2019-06-06 | Novel cell recovery device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210134124U (en) |
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2019
- 2019-06-06 CN CN201920850941.2U patent/CN210134124U/en active Active
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