CN210108829U - Sample concentration device for capillary electrophoresis - Google Patents
Sample concentration device for capillary electrophoresis Download PDFInfo
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- CN210108829U CN210108829U CN201920593578.0U CN201920593578U CN210108829U CN 210108829 U CN210108829 U CN 210108829U CN 201920593578 U CN201920593578 U CN 201920593578U CN 210108829 U CN210108829 U CN 210108829U
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- electrophoresis
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- 238000005251 capillar electrophoresis Methods 0.000 title claims abstract description 25
- 238000001962 electrophoresis Methods 0.000 claims abstract description 26
- 230000005611 electricity Effects 0.000 claims abstract description 7
- 239000004020 conductor Substances 0.000 claims abstract description 5
- 239000000523 sample Substances 0.000 claims description 103
- 239000002184 metal Substances 0.000 claims description 26
- 239000003792 electrolyte Substances 0.000 claims description 16
- 239000012488 sample solution Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 5
- 150000002500 ions Chemical class 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 abstract description 5
- 238000002347 injection Methods 0.000 description 12
- 239000007924 injection Substances 0.000 description 12
- 238000000926 separation method Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 4
- 230000003321 amplification Effects 0.000 description 3
- 238000001649 capillary isotachophoresis Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 230000001052 transient effect Effects 0.000 description 3
- 239000004696 Poly ether ether ketone Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 229920002530 polyetherether ketone Polymers 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000010963 304 stainless steel Substances 0.000 description 1
- 229910000589 SAE 304 stainless steel Inorganic materials 0.000 description 1
- 238000005515 capillary zone electrophoresis Methods 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000012468 concentrated sample Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010891 electric arc Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 239000004811 fluoropolymer Substances 0.000 description 1
- 229920002313 fluoropolymer Polymers 0.000 description 1
- 239000005350 fused silica glass Substances 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000005220 pharmaceutical analysis Methods 0.000 description 1
- 238000004094 preconcentration Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
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Abstract
The utility model discloses a sample enrichment facility of capillary electrophoresis, characteristics are: including connecting the cavity, the electrophoresis unit, at least one advances kind of unit, first high voltage power supply and second high voltage power supply, it adopts conducting material to connect the cavity, it is connected with first high voltage power supply electricity to connect the cavity, it is linked together with the one end of advancing kind of unit and electrophoresis unit's one end respectively to connect the cavity, it is connected with second high voltage power supply electricity to advance the other end of kind of unit, the electrophoresis unit is connected with the detector, the other end ground connection of electrophoresis unit, connect between cavity and the kind of unit, it all is provided with airtight mechanism to connect between cavity and the electrophoresis unit, the advantage is: by constructing the low potential at the connecting node, the sample ions are concentrated towards the connecting node, the sample introduction volume of the capillary can be increased to the volume of a plurality of capillary columns, and the sample introduction volume and the detection limit of capillary electrophoresis are greatly improved.
Description
Technical Field
The utility model relates to a capillary electrophoresis field especially relates to a sample enrichment facility of capillary electrophoresis.
Background
Capillary electrophoresis has the advantages of high separation efficiency, high separation speed, capability of separating isomers and the like, and is widely applied to the fields of proteomics, metabonomics, pharmaceutical analysis, food detection and the like.
Compared with liquid chromatography, the sample injection volume is one of the main reasons for limiting the further wide application of capillary electrophoresis. The sample injection volume of the traditional capillary zone electrophoresis is only about 1 percent of the volume of the capillary column, and the sample injection volume is only 7 nanoliters according to the typical capillary with the inner diameter of 30 micrometers and the length of 1 meter. Increasing the inner diameter of the capillary or increasing the length of the capillary can increase the sample volume. However, since increasing the inner diameter of the capillary tube leads to a decrease in the heat dissipation capability of the quartz capillary tube and thus to a decrease in the separation efficiency, the inner diameter of the capillary tube is generally 100 μm or less. In order to ensure the separation efficiency, the length of the capillary is increased, and the voltage value of a high-voltage power supply for electrophoretic separation is required to be correspondingly increased so as to keep the electric field intensity of separation constant, but the safety problems such as electric arc and the like are easily caused by overhigh voltage, so that the electrophoretic voltage of less than 30 kilovolts is generally adopted, and the length of the capillary is about 1 meter.
The preconcentration of the sample is an effective method for increasing the sample injection volume of capillary electrophoresis, and includes methods such as transient capillary isotachophoresis (transient capillary isotachophoresis), field amplification stacking (field amplification stacking), dynamic pH junction (dynamic pH junction), and large volume sample stacking (large volume sampling). The sample injection volume of the field amplification stacking method is generally below 5% of the capillary column volume, the sample injection volume of the transient capillary isotachophoresis and dynamic pH junction methods is generally below 50% of the capillary column volume, and the large-capacity sample stacking method can improve the sample injection volume to be close to 100% of the capillary column volume. However, the sample cannot be completely separated due to the higher sample volume, and the sample volume of these existing sample concentration methods cannot exceed 1 capillary column volume, so the sample volume of capillary electrophoresis is still limited compared to liquid chromatography.
Disclosure of Invention
In order to solve the deficiencies existing in the prior art, the utility model provides a sample concentration device for capillary electrophoresis can improve the appearance volume and the detection limit of advancing of capillary electrophoresis by a wide margin.
The utility model provides a technical scheme that above-mentioned technical problem adopted does: the utility model provides a sample enrichment facility of capillary electrophoresis, advances kind unit, first high voltage power supply and second high voltage power supply including connecting cavity, electrophoresis unit, at least one, the connection cavity adopt conducting material, the connection cavity with first high voltage power supply electricity connect, the connection cavity respectively with advance the one end of kind unit with the one end of electrophoresis unit be linked together, the other end of advancing kind unit with second high voltage power supply electricity connect, the electrophoresis unit be connected with the detector, the other end ground connection of electrophoresis unit, the connection cavity with advance kind unit between the cavity connect the cavity with the electrophoresis unit between all be provided with the airtight mechanism that is used for guaranteeing the gas tightness.
In some embodiments, the electrophoresis unit includes a first capillary and a first sample bottle, the connection chamber is provided with a first opening, the first opening is communicated with one end of the first capillary, the other end of the first capillary passes through the detector and then is inserted into the background electrolyte contained in the first sample bottle, and the background electrolyte contained in the first sample bottle is grounded through a lead.
In some embodiments, the sample injection unit comprises a second capillary and a second sample bottle, the connection chamber is provided with a second opening corresponding to the first opening, the second opening is communicated with one end of the second capillary, the other end of the second capillary is inserted into the sample solution or the background electrolyte contained in the second sample bottle, and the second high-voltage power supply is connected with the background electrolyte contained in the second sample bottle through a wire.
In some embodiments, the air-tight mechanism comprises a sleeve and a ferrule adapter, the sleeve is disposed between the first opening and the first capillary and between the second opening and the second capillary, and the ferrule adapter is disposed outside the sleeve for clamping the sleeve. Thereby serving to ensure airtightness between the connection chamber and the respective capillaries.
In some embodiments, the connection chamber is formed by a middle portion of a metal two-way, a first end portion of the metal two-way includes the first opening, a second end portion of the metal two-way includes the second opening, and an outer wall of the middle portion of the metal two-way is connected to the first high-voltage power supply through a wire. The structure of the metal two-way can well realize that two sections of capillaries are connected by a connecting cavity in a narrow space, low potential is constructed at the connecting junction, and the function of continuously converging and concentrating sample ions in the capillaries to the connecting junction of the low potential is realized.
In some embodiments, three the introduction unit, the introduction unit include second capillary and second sample bottle, the connection cavity constitute by the intermediate part of a metal cross, the first end of metal cross include first opening, first opening with the one end of first capillary be linked together, second end, third end and the fourth end of metal cross on be provided with the second opening respectively, three the second opening respectively with three the second capillary be linked together, three the other end of second capillary insert respectively three the second sample bottle hold the liquid in, second high voltage power supply connect respectively through the wire the second sample bottle hold liquid. This metal cross can realize better connecting the cavity with the connection of a narrow and small space with the multistage capillary, through constructing the low potential at the connection node for the concentrated function is constantly assembled to the connection node of low potential to the sample ion in the capillary, can also use the appearance unit of advancing of different quantity to connect the multistage capillary in other embodiments, advance the appearance in order to accomplish the multistage formula, and construct the low potential at the connection node.
In some embodiments, the air-tight mechanism comprises a sleeve and a ferrule connector, the sleeve is arranged between the first opening and the first capillary tube and between the three second openings and the corresponding second capillary tubes, and the ferrule connector is sleeved outside the sleeve for clamping the sleeve.
In some embodiments, the detector is an ultraviolet detector.
Compared with the prior art, the utility model has the advantages of: two or more sections of capillaries are connected by a connecting cavity in a narrow space, low potential is constructed at the connecting node, so that sample ions in the capillaries are continuously converged and concentrated to the connecting node of the low potential, and after the concentration is finished, high voltage is applied to separate the sample ions along the capillaries of the electrophoresis unit, so that the sample introduction volume of the capillaries can be increased to the volume of a plurality of capillary columns, and the sample introduction volume and the detection limit of capillary electrophoresis are greatly improved; and the utility model discloses simple structure easily assembles and uses.
Drawings
FIG. 1 is a schematic structural diagram of a first embodiment of a sample concentrating device for capillary electrophoresis according to the present invention;
fig. 2 is a schematic structural diagram of a second embodiment of the sample concentrating device for capillary electrophoresis according to the present invention.
The device comprises a connecting chamber 1, a first opening 11, a second opening 12, an electrophoresis unit 2, a first capillary 21, a first sample bottle 22, a sample introduction unit 3, a second capillary 31, a second sample bottle 32 for containing background electrolyte, a second sample bottle 33 for containing sample solution, a first high-voltage power supply 4, a second high-voltage power supply 5, a detector 6, a sleeve 7, a ferrule connector 8, a metal two-way 9 and a metal four-way 10.
Detailed Description
The following will explain in detail the sample concentrating device for capillary electrophoresis according to the present invention with reference to the accompanying drawings, but the present invention is not limited thereto.
Example one
As shown in fig. 1, a sample concentration device for capillary electrophoresis, including electrically conductive connection cavity 1, electrophoresis unit 2, at least one sample introduction unit 3, first high voltage power supply 4 and second high voltage power supply 5, connection cavity 1 is made of conductive material, connection cavity 1 is electrically connected with first high voltage power supply 4, connection cavity 1 is respectively linked together with one end of sample introduction unit 3 and one end of electrophoresis unit 2, the other end of sample introduction unit 3 is electrically connected with second high voltage power supply 5, electrophoresis unit 2 is connected with detector 6, the other end of electrophoresis unit 2 is grounded, connect between cavity 1 and sample introduction unit 3, it is provided with the airtight mechanism that is used for guaranteeing the gas tightness to connect between cavity 1 and the electrophoresis unit 2.
In this embodiment, the electrophoresis unit 2 includes a first capillary 21 and a first sample bottle 22, a first opening 11 is disposed on the connection chamber 1, the first opening 11 is communicated with one end of the first capillary 21, the other end of the first capillary 21 passes through the detector 6 and then is inserted into the background electrolyte contained in the first sample bottle 22, and the background electrolyte contained in the first sample bottle 22 is grounded through a conducting wire.
In this embodiment, the sample introduction unit 3 includes a sample introduction unit 3, the sample introduction unit 3 includes a second capillary 31 and a second sample bottle, a second opening 12 is disposed on the connection chamber 1 relative to the first opening 11, the second opening 12 is communicated with one end of the second capillary 31, the other end of the second capillary 31 is inserted into a second sample bottle 33 (for sample introduction) containing a sample solution or a second sample bottle 32 (for sample concentration and sample separation) containing a background electrolyte, and the second high voltage power supply 5 is connected to the background electrolyte contained in the second sample bottle through a wire. The capillary tubes are fused quartz capillary tubes with the inner diameter of 20-100 micrometers, the outer diameter of 150-360 micrometers and the length of less than 1 meter.
In this embodiment, the airtight mechanism includes a sleeve 7 and a ferrule adapter 8, the sleeve 7 is disposed between the first opening 11 and the first capillary 21 and between the second opening 12 and the second capillary 31, and the ferrule adapter 8 is sleeved outside the sleeve 7 for clamping the sleeve 7. The sleeve 7 may be made of FEP (fluoropolymer) material for ensuring airtightness between the capillary and the connection chamber; the ferrule adapter 8 may be made of PEEK (polyetheretherketone) material, but is not limited thereto.
In this embodiment, the connection chamber 1 is made of a conductive material, specifically, the connection chamber 1 is formed by a middle portion of a metal two-way 9, a first end portion of the metal two-way 9 includes a first opening 11, a second end portion of the metal two-way 9 includes a second opening 12, and an outer wall of the middle portion of the metal two-way 9 is connected to the first high voltage power supply 4 through a wire. The metal two-way 9 can be made of 304 stainless steel material, but is not limited to this.
Example two
As shown in FIG. 2, the other structure of a sample concentrating device for capillary electrophoresis is the same as that of the first embodiment, except that: in this embodiment, the sample injection unit includes three sample injection units 3, the connection chamber 1 is formed by an intermediate portion of a metal cross 10, a first end portion of the metal cross 10 includes a first opening 11, the first opening 11 is communicated with one end of a first capillary 21, a second end portion, a third end portion and a fourth end portion of the metal cross 10 are respectively provided with a second opening 12, three second openings 12 are respectively communicated with three second capillaries 31, the other ends of the three second capillaries 31 are respectively inserted into a liquid container of three second sample bottles, the second sample bottles can be second sample bottles 33 (for sample injection) containing sample solutions or second sample bottles 32 (for sample concentration and sample separation) containing background electrolytes, and the second high voltage power supply 5 is respectively connected to the second sample bottles 32 containing background electrolytes through wires.
In this embodiment, the sleeve 7 is disposed between the first opening 11 and the first capillary 21 and between the three second openings 12 and the corresponding second capillaries 31, and the ferrule adapter 8 is sleeved outside the sleeve 7 for clamping the sleeve 7.
EXAMPLE III
Adopt the utility model discloses a method that sample enrichment facility of capillary electrophoresis carries out sample concentration, including following step:
1) sample introduction: loading a sample solution into a second sample bottle, and filling the first capillary and the second capillary with the sample solution in a gas pressure sample injection mode;
2) and (3) concentrating a sample: loading background electrolyte on a first sample bottle and a second sample bottle, outputting high voltage with negative polarity by a first high-voltage power supply, adjusting the output voltage value of a second high-voltage power supply to zero, forming the lowest potential in a connecting cavity between a first capillary tube and a second capillary tube, concentrating sample cations into the connecting cavity, and after waiting for a sufficient time, concentrating sample ions in the first capillary tube and the second capillary tube into the connecting cavity;
3) sample separation: and applying high voltage with positive polarity to the first high-voltage power supply and the second high-voltage power supply to separate the concentrated sample ions in the connecting chamber in the first capillary, wherein the sample ions flow through the detector and obtain a detection signal by the detector.
In this embodiment, the high voltage power supply adopts a dc high voltage power supply with switchable positive and negative polarities, and the loaded high voltage value is 10-30 kv.
The utility model relates to a sample enrichment facility of capillary electrophoresis is connected with the connection cavity in a narrow and small space through with two sections or multistage capillaries, through constructing the low potential in connection node department, make the sample ion in the capillary constantly assemble the concentration to the connection node department of low potential, treat that the concentration finishes, reapply high-tension electricity and make the sample ion along the capillary separation of electrophoresis unit wherein, can make the appearance volume of advancing of capillary increase to a plurality of capillary column volumes, thereby improve the appearance volume of advancing of capillary electrophoresis and detect the limit by a wide margin.
It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and the present invention can also be modified in materials and structures, or replaced by technical equivalents. Therefore, all structural equivalents which may be made by applying the present invention to the specification and drawings, or by applying them directly or indirectly to other related technical fields, are intended to be encompassed by the present invention.
Claims (8)
1. The utility model provides a sample enrichment facility of capillary electrophoresis, its characterized in that, including connecting cavity, electrophoresis unit, at least one advance kind unit, first high voltage power supply and second high voltage power supply, the connection cavity adopt conducting material, the connection cavity with first high voltage power supply electricity connect, the connection cavity respectively with advance the kind unit one end with the one end of electrophoresis unit be linked together, the kind unit the other end with second high voltage power supply electricity connect, the electrophoresis unit be connected with the detector, the other end ground connection of electrophoresis unit, the connection cavity with advance kind between the unit the connection cavity with the electrophoresis unit between all be provided with the airtight mechanism that is used for guaranteeing the gas tightness.
2. The apparatus according to claim 1, wherein the electrophoresis unit comprises a first capillary and a first sample bottle, the connection chamber has a first opening, the first opening is connected to one end of the first capillary, the other end of the first capillary passes through the detector and is inserted into the background electrolyte contained in the first sample bottle, and the background electrolyte contained in the first sample bottle is grounded through a conducting wire.
3. The capillary electrophoresis sample concentration device according to claim 2, comprising one sample introduction unit, wherein the sample introduction unit comprises a second capillary and a second sample bottle, the connection chamber is provided with a second opening opposite to the first opening, the second opening is communicated with one end of the second capillary, the other end of the second capillary is inserted into the sample solution or the background electrolyte contained in the second sample bottle, and the second high voltage power supply is connected with the background electrolyte contained in the second sample bottle through a lead.
4. The apparatus according to claim 3, wherein the airtight mechanism comprises a sleeve and a ferrule connector, the sleeve is disposed between the first opening and the first capillary and between the second opening and the second capillary, and the ferrule connector is disposed outside the sleeve for clamping the sleeve.
5. The apparatus according to claim 3, wherein the connection chamber is formed by a middle portion of a metal two-way, a first end portion of the metal two-way includes the first opening, a second end portion of the metal two-way includes the second opening, and an outer wall of the middle portion of the metal two-way is connected to the first high voltage power supply through a wire.
6. The capillary electrophoresis sample concentration device according to claim 2, comprising three sample introduction units, wherein each sample introduction unit comprises a second capillary and a second sample bottle, the connection chamber comprises a middle part of a metal four-way, a first end part of the metal four-way comprises the first opening, the first opening is communicated with one end of the first capillary, second openings are respectively arranged on a second end part, a third end part and a fourth end part of the metal four-way, the three second openings are respectively communicated with the three second capillaries, the other ends of the three second capillaries are respectively inserted into the three containing liquids of the second sample bottles, and the second high-voltage power supply is respectively connected with the containing liquids of the second sample bottles through conducting wires.
7. The apparatus according to claim 6, wherein the air-tight mechanism comprises a sleeve and a ferrule connector, the sleeve is disposed between the first opening and the first capillary and between the three second openings and the corresponding second capillaries, and the ferrule connector is disposed outside the sleeve for clamping the sleeve.
8. The apparatus according to claim 1, wherein the detector is an ultraviolet detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920593578.0U CN210108829U (en) | 2019-04-28 | 2019-04-28 | Sample concentration device for capillary electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920593578.0U CN210108829U (en) | 2019-04-28 | 2019-04-28 | Sample concentration device for capillary electrophoresis |
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| Publication Number | Publication Date |
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| CN210108829U true CN210108829U (en) | 2020-02-21 |
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| CN201920593578.0U Withdrawn - After Issue CN210108829U (en) | 2019-04-28 | 2019-04-28 | Sample concentration device for capillary electrophoresis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110044680A (en) * | 2019-04-28 | 2019-07-23 | 宁波大学 | A kind of sample concentration device and method of Capillary Electrophoresis |
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2019
- 2019-04-28 CN CN201920593578.0U patent/CN210108829U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110044680A (en) * | 2019-04-28 | 2019-07-23 | 宁波大学 | A kind of sample concentration device and method of Capillary Electrophoresis |
| CN110044680B (en) * | 2019-04-28 | 2024-02-23 | 宁波大学 | Sample concentration device and method for capillary electrophoresis |
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