CN210085466U - Terahertz wave vertical exposure system for cell experiment - Google Patents
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- CN210085466U CN210085466U CN201822177471.6U CN201822177471U CN210085466U CN 210085466 U CN210085466 U CN 210085466U CN 201822177471 U CN201822177471 U CN 201822177471U CN 210085466 U CN210085466 U CN 210085466U
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Abstract
The utility model provides a terahertz wave exposes system perpendicularly for cell experiment, include: the device comprises a terahertz wave source, a converging lens, a lifting platform, a thermostat, a ventilation box and a carbon dioxide gas cylinder, wherein a light emitting port of the terahertz wave source is placed upwards; the converging lens is arranged above the light-emitting port; the lifting rack is arranged above the convergent lens, and a first through hole is formed in the table top of the lifting rack; the constant temperature box is arranged on the lifting platform, a second through hole is formed in the bottom wall of the constant temperature box, and a ventilation inlet and a ventilation outlet are formed in the side wall of the constant temperature box; an air exhaust fan is arranged in the constant temperature box; an ultraviolet disinfection lamp is arranged in the air-changing box, an air inlet on the side wall of the air-changing box is connected with an air-changing outlet, and an air outlet is connected with an air-changing inlet; the carbon dioxide gas cylinder is connected with the scavenging box; the light emitting port of the terahertz wave source, the converging lens, the first through hole and the central axis of the second through hole are overlapped. The exposure system has the advantages of stable terahertz wave, short optical path and low attenuation, and can remarkably improve the repeatability and precision of an exposure experiment.
Description
Technical Field
The utility model belongs to electromagnetic radiation biological effect field and experimental technical equipment field, particularly, the utility model relates to a terahertz wave exposes system perpendicularly for cell experiment.
Background
With the technological progress, terahertz waves gradually enter the production and life of people, and the electromagnetic waves with heat effect, high penetrability (nonpolar substances and nonmetal), high information carrying capacity and low energy have huge application prospects. Humans are increasingly exposed to terahertz radiation environments. The overlap of the terahertz wave frequency with the resonance frequency of the biological macromolecule also makes guesswork that it may have a unique non-thermal effect. The biological effect of terahertz waves has gradually attracted attention.
In the research of terahertz wave cell biological effect, a method of irradiating from the bottom of a culture dish is mostly adopted because of the absorption effect of a culture solution on terahertz waves. In a traditional terahertz wave irradiation system, terahertz waves are horizontally emitted by a terahertz wave source and can be vertically irradiated from bottom to top only by being reflected by a reflector. In such a reflection-type optical path, the requirement for the stability of the entire optical path is extremely high, and any slight error causes a great change in the optical path, and complicated maintenance and testing are required in use. And because the reflector must be arranged, the whole length of the optical path is longer, and the longer the optical path is, the more the terahertz wave is attenuated under the influence of water molecules in the air. The factors influence the popularization and smooth implementation of the terahertz wave biological effect research. Therefore, it is necessary to establish a high-precision low-attenuation terahertz wave cell exposure system.
SUMMERY OF THE UTILITY MODEL
The present invention aims at solving at least one of the technical problems in the related art to a certain extent. Therefore, an object of the utility model is to provide a terahertz wave exposes system perpendicularly for cell experiment, this terahertz wave exposes system perpendicularly and can simplify the optical path that terahertz cell exposed in the experiment, improves the precision of experiment, and this exposes system perpendicularly and has been successfully applied to terahertz wave in the influence research to neural information transmission.
According to an aspect of the utility model, the utility model provides a terahertz wave exposes system perpendicularly for cell experiment is proposed, according to the utility model discloses an embodiment, this terahertz wave exposes system perpendicularly includes:
the terahertz wave source is placed with a light emitting port upwards so that the emitted terahertz waves are vertically upwards;
the converging lens is arranged above the light-emitting port so as to convert the terahertz waves into terahertz parallel waves;
the terahertz parallel wave focusing device comprises a terahertz wave source, a converging lens, a lifting table, a focusing lens and a focusing lens, wherein the terahertz wave source is arranged on the top of the terahertz wave source;
the bottom wall of the constant temperature box is provided with a second through hole which is sealed by a light-transmitting material, and the side wall of the constant temperature box is provided with a ventilation inlet and a ventilation outlet; the thermostat is arranged on the table top of the lifting table, and the second through hole is overlapped with the first through hole; a constant temperature heating plate, a thermometer and an air exhaust fan are arranged in the constant temperature box, and the air exhaust fan is connected with the air exchange inlet; the incubator is suitable for culturing cells, and a cell culture dish for culturing cells is placed above the second through hole;
the air exchange box is internally provided with an ultraviolet disinfection lamp and is provided with an air inlet and an air outlet, the air inlet is connected with the air exchange outlet, and the air outlet is connected with the air exchange inlet;
the carbon dioxide gas cylinder is connected with the ventilation box;
the light emitting port of the terahertz wave source, the converging lens, the first through hole and the central axis of the second through hole are overlapped.
Therefore, the utility model discloses a perpendicular exposure system of terahertz wave for cell experiment can furthest's simplification terahertz wave shine to the light path of cultivateing the cell, makes terahertz wave after sending from the wave source through assemble become the parallel light after, upwards shines the cell from the culture dish bottom after the first through-hole passes the printing opacity material board from the second through-hole of thermostated container bottom. The constant temperature box can maintain the temperature required by the cell growth, and the ventilation box can maintain the carbon dioxide concentration and the sterile air required by the cell growth. The vertical terahertz wave exposure system has the characteristics of high precision and low attenuation, and can be successfully applied to biological effect research of influence of terahertz waves on neuron information transfer.
In addition, the vertical terahertz wave exposure system for cell experiments according to the above embodiments of the present invention may also have the following additional technical features:
in the present invention, the terahertz wave vertical exposure system of the above embodiment further includes: the terahertz wave source support is suitable for bearing the terahertz wave source, so that a light emitting opening of the terahertz wave source faces upwards.
The utility model discloses in, the lens that assembles passes through the liftable support and supports.
The utility model discloses in, the convergent lens with the distance of the light-emitting mouth of terahertz wave source does the focus of convergent lens.
In the present invention, the distance between the converging lens and the top surface of the lifting platform is 5-30 cm.
The utility model discloses in, the lens that assembles with the distance of elevating platform top surface is 5 cm.
The utility model discloses in, take a breath the entry with the export of taking a breath sets up respectively on two relative lateral walls of thermostated container.
The utility model discloses in, the air inlet with take a breath the export with the gas outlet with the entry of taking a breath links to each other or the rubber tube links to each other through the PVC pipe.
The utility model discloses in, first through-hole with the diameter of second through-hole is not less than the diameter of lens assembles.
In the present invention, the output frequency of the terahertz wave source is 96 gigahertz-2.1 terahertz, and the output power is not more than 65 milliwatts.
Drawings
Fig. 1 is a schematic structural diagram of a vertical terahertz wave exposure system for cell experiments according to an embodiment of the present invention.
FIG. 2 is a graph of primary hippocampal neuron growth after 0.16THz/50mW terahertz wave irradiation (inverted microscope, 400-fold);
FIG. 3 is a graph showing the results of primary hippocampal neuron Sholl analysis after irradiation with 0.16THz/50mW terahertz wave.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
According to an aspect of the utility model, the utility model provides a terahertz wave exposes system perpendicularly for cell experiment is proposed, according to the utility model discloses an embodiment, this terahertz wave exposes system perpendicularly includes: the terahertz wave source 100, the converging lens 200, the lifting platform 300, the thermostat 400, the ventilation box 500 and the carbon dioxide gas bottle 600.
The terahertz wave source 100 is vertically placed, that is, the light emitting port 110 of the terahertz wave source 100 is placed upwards, so that the terahertz wave is emitted vertically upwards. The condensing lens 200 is disposed above the light emitting port 110 to convert the terahertz waves into terahertz parallel waves. The elevating platform 300 is erected above the terahertz wave source 100 and the converging lens 200, and a first through hole 310 is arranged on the table-board of the elevating platform 300 so that the terahertz parallel waves can pass through the hole.
A second through hole 410 is formed on the bottom wall of the incubator 400, the second through hole 410 is sealed by a light-transmitting material 420, and a ventilation inlet 430 and a ventilation outlet 440 are formed on the side wall of the incubator 400; the thermostat 400 is disposed on the top of the lifting table 300, and the second through hole 410 is overlapped with the first through hole 310; a constant temperature heating plate 450, a thermometer 460 and an air suction fan 470 are arranged in the constant temperature box 400, and the air suction fan 470 is connected with the air exchange inlet 430; the inside of the incubator 400 is adapted to culture cells, and a cell culture dish for culturing cells is placed above the second through-hole 410.
The ultraviolet ray sterilizing lamp 510 is provided in the air-exchanging chamber 500, the air-exchanging chamber 500 has an air inlet 520 and an air outlet 530, the air inlet 520 is connected to the air-exchanging outlet 440, and the air outlet 530 is connected to the air-exchanging inlet 430. A carbon dioxide cylinder 600 is connected to the ventilation chamber 500. The central axes of the light emitting port 110 of the terahertz wave source 100, the converging lens 200, the first through hole 310 and the second through hole 410 are overlapped.
According to the terahertz wave vertical exposure system for cell experiments of the present invention, firstly, the terahertz wave source 100 is vertically placed so that the terahertz wave is emitted vertically upward, and further the collecting lens 200 is disposed above the light emitting opening 110 so as to convert the terahertz wave into the terahertz parallel wave; the constant temperature box 400 for culturing the cells is arranged above the collecting lens 200 through the lifting platform 300, and through holes are respectively arranged on the lifting platform and the bottom wall of the constant temperature box 400, so that terahertz parallel waves passing through the collecting lens 200 can be smoothly irradiated to the cells in the constant temperature box 400, and further terahertz wave exposure experiment research is carried out on the cells.
Therefore, the utility model discloses a terahertz wave exposes system structural design is simple effective perpendicularly for cell experiments for above-mentioned embodiment, and terahertz wave only needs after sending from the wave source to become the parallel light through assembling after, can directly shine the cell in the thermostated container. Further, the light path and the optical path of the terahertz wave irradiating the cultured cells are simplified to the maximum extent, and the energy loss of the terahertz wave can be effectively avoided. Therefore, the utility model discloses terahertz wave exposes system perpendicularly has high accuracy, low decay characteristics, can successfully be applied to in the biological effect research of terahertz wave to neuron information transfer influence.
The terahertz wave vertical exposure system for cell experiments according to an embodiment of the present invention will be described in detail with reference to fig. 1.
As shown in fig. 1, firstly, the terahertz wave source 100 is vertically placed, that is, the light emitting port 110 of the terahertz wave source 100 is placed upward, so that the terahertz wave is emitted vertically upward. According to the utility model discloses a concrete embodiment, the perpendicular exposure system of terahertz wave of above-mentioned embodiment can also further include: the terahertz wave source support 700 is suitable for bearing the terahertz wave source 100, so that a light emitting opening of the terahertz wave source faces upwards. Generally, the terahertz wave source 100 is horizontally disposed, and the terahertz wave emitted by the terahertz wave source is also horizontally emitted. The terahertz wave source 100 can be effectively placed vertically and stably by arranging the terahertz wave source support 700. Therefore, the terahertz wave can directly irradiate the cells, the optical path is shortened, and the attenuation of the terahertz wave is reduced.
According to the embodiment of the present invention, as shown in fig. 1, the converging lens 200 is disposed above the light emitting opening 110 so as to convert the terahertz waves into terahertz parallel waves. The converging lens 200 can effectively convert terahertz waves into terahertz parallel waves, so that terahertz divergence can be avoided, and energy attenuation is avoided. And the terahertz parallel wave is converted into the terahertz parallel wave, so that the stability of the terahertz wave can be improved, the irradiation distance of the terahertz parallel wave can be prolonged, and the terahertz parallel wave can be effectively irradiated to cultured cells in the incubator.
According to the specific embodiment of the present invention, the convergent lens 200 may be supported by the lifting bracket 800. And thus the position of the condensing lens 200 can be adjusted more conveniently and flexibly. Specifically, the distance of the light emitting port 110 of the converging lens 200 and the terahertz wave source 100 should be equal to the focal length of the converging lens 200, and is determined according to the focal length of the lens used, the embodiment of the present invention provides a lens focal length of 5cm, which is too large or too small in distance and can affect the parallelism of the terahertz wave and cause the energy waste of the terahertz wave.
According to the specific embodiment of the present invention, as shown in fig. 1, the lifting platform 300 is erected above the terahertz wave source 100 and the converging lens 200, and the table-board of the lifting platform 300 is provided with the first through hole 310, so that the terahertz parallel wave passes through. From this, can place thermostated container 400 in the top of collecting lens 200 through setting up elevating platform 300, and then can make things convenient for terahertz parallel wave directly to shine to the cultured cell in thermostated container 400 on.
According to a specific example of the present invention, the height of the elevating platform 300 is adjustable, and thus, it is possible to conveniently adjust such that the collective lens 200 maintains a suitable distance from the thermostat 400 on the elevating platform 300. Specifically, the height of the lifting platform 300 can be adjusted so that the distance between the converging lens 200 and the top surface of the lifting platform is 5cm-30 cm. If the distance is too large, the optical path is prolonged, and therefore the loss of terahertz wave energy in the optical path is increased. According to the utility model discloses specific embodiment, the distance of convergent lens 200 and elevating platform top surface is the better the less, and preferred 5cm is optimum distance.
According to the embodiment of the present invention, as shown in fig. 1, a second through hole 410 is formed on the bottom wall of the incubator 400, and the second through hole 410 is sealed by a transparent material 420, and a ventilation inlet 430 and a ventilation outlet 440 are formed on the side wall of the incubator 400; the thermostat 400 is disposed on the top of the lifting table 300, and the second through hole 410 is overlapped with the first through hole 310; a constant temperature heating plate 450, a thermometer 460 and an air suction fan 470 are arranged in the constant temperature box 400, and the air suction fan 470 is connected with the air exchange inlet 430; a cell culture dish adapted to culture cells is placed above the second through-hole 410 in the incubator 400; an ultraviolet disinfection lamp 510 is arranged in the air-changing box 500, the air-changing box 500 is provided with an air inlet 520 and an air outlet 530, the air inlet 520 is connected with the air-changing outlet 440, and the air outlet 530 is connected with the air-changing inlet 430; a carbon dioxide cylinder 600 is connected to the ventilation chamber 500.
Therefore, the utility model discloses the system that exposes through additionally setting up ventilation box 500 and providing the air of suitable carbon dioxide concentration for thermostated container 400 to carry out circulation germicidal treatment to the air. Therefore, the air quality in the incubator can be obviously improved, and a high-quality environment is provided for cell culture.
According to the utility model discloses a concrete embodiment, the entry of taking a breath with the export of taking a breath sets up respectively on two lateral walls that the thermostated container is relative. Therefore, the air in the constant temperature box 400 can be effectively ensured to be completely circularly replaced, and meanwhile, the replacement efficiency can be further improved. In addition, the suction fan 470 is connected to the ventilation inlet 430, so that it is possible to ensure that the sterilizing air can be efficiently introduced into the inside of the incubator 400.
According to the utility model discloses a concrete embodiment, the air inlet with take a breath the export with the gas outlet with the entry of taking a breath passes through PVC pipe or rubber tube and links to each other. Thereby facilitating the connection between the ventilation box 500 and the incubator 400, and is not limited by the location of the ventilation box and the incubator.
According to the embodiment of the present invention, when the thermostat 400 is disposed on the top of the lifting platform 300, it is necessary to ensure that the second through hole 410 coincides with the first through hole 310. And thus the terahertz parallel waves can be effectively transmitted. Specifically, the diameters of the first through hole and the second through hole are not smaller than the diameter of the condenser lens. And then can guarantee terahertz parallel light can all pass first through-hole and second through-hole, avoid terahertz parallel light extravagant now.
The utility model discloses in the system that exposes of above-mentioned embodiment, the axis coincidence of luminous mouthful 110, convergent lens 200, first through-hole 310 and second through-hole 410 of terahertz wave source 100. It is thereby possible to ensure that the terahertz light is irradiated into oven 400 at the maximum. A culture dish for culturing cells in the incubator 400 needs to be placed above the second through hole, and then the terahertz parallel light in the incubator 400 can be directly irradiated to the cells.
According to the embodiment of the present invention, the output frequency of the terahertz wave source 100 can be 96 gigahertz to 2.1 terahertz, and the output power is not more than 65 milliwatts. Thereby being effectively used for the exposure of the cells to be tested.
According to the utility model discloses a terahertz wave vertical exposure system for cell experiment is applicable in shining of adherence growth cell. Specifically, the adherently growing cells may include at least one selected from the group consisting of primary hippocampal neurons, primary cortical neurons, primary cerebellar neurons, primary brainstem neurons, MN9D cells, HT22 cells, and PC12 cells.
According to the specific embodiment of the present invention, primary hippocampal neurons are irradiated by using this system. Experiments show that 0.16THz/50mW terahertz waves can influence the transmitter metabolic activity of primary hippocampal neurons immediately after irradiation, and can cause the increase and change of excitatory amino acid glycine. Therefore, the utility model discloses the system of exposing of above-mentioned embodiment can be used for primary hippocampal neuron's research effectively, and should expose the advantage that the system has terahertz wave stability, low decay, and it is high to expose experimental repeatability.
Examples
The following describes in detail the related experiments performed using the vertical exposure system 10 for cell experiments using terahertz waves as described above.
(A) Measuring the temperature change of the culture dish after terahertz wave radiation: QS2-180 terahertz emission source (microtechniquent, USA) is adopted, the frequency range is 0.096-0.18 THz, and the power range is 1-65 mW. And 0.16THz terahertz wave is adopted, and the corresponding power is 50 mW. As shown in the attached figure 1, after the terahertz waves are emitted, the terahertz waves are converged by the converging lens, and parallel light irradiates into the culture dish from the bottom of the culture dish. The temperature change was measured using a TH-212 Intelligent temperature recorder (Beijing gull-Olympic instruments, Inc., China), the temperature probe was placed in a cell culture dish and immersed in the cell culture medium to make it fully contact the bottom of the dish, and then the dish was placed in an external incubator for 5h to allow the temperature of the culture to reach the incubator stable temperature. Adjusting the optical path to be vertical and stable, then starting a terahertz wave source, dynamically observing temperature change at the time points of irradiation for 1, 2, 3, 4, 5, 6, 10, 20, 30, 40, 50 and 60min, and well recording. The results are shown in Table 1.
TABLE 10.16 THz/50mW THz wave changes in the temperature of the dish after irradiation
As can be seen from Table 1, the temperature change in the dish slowly increased with 60min irradiation, with an overall increase of about 0.12 ℃. It can be seen from the numerical value that the temperature variation range is not large, the influence of the thermal effect on the cells under the experimental condition is considered to be almost negligible, and the non-thermal biological effect of the terahertz waves is researched.
(B) Carrying out terahertz wave radiation on primary hippocampal neurons: taking Wistar suckling mouse within 12h of newborn, soaking in 75% alcohol for disinfection. Cutting off the head under aseptic condition, and collecting brain. Under a dissecting microscope, the hippocampal brain region of the brain was dissected away with ophthalmological forceps. Then digesting and dispersing the separated brain region, counting cells, diluting the cell suspension to 5 × 10 with planting solution5The density of/m L, inoculating to poly-lysine coated cell culture dish, and culturing in cell culture box. After 24h, the medium was changed, the planting solution was completely aspirated, and about 2ml of the feeding solution was added. And adding cytarabine with the final concentration of 3-5 mu g/m L to the culture dish on the 3 rd day of culture, changing the liquid after 24h, changing the liquid for 2 times every week, culturing for one week, and culturing for one week, wherein during irradiation, the cell culture dish is placed on a light-transmitting plastic sealing plate on a light path passing port at the bottom of an external incubator for culture, the position of the cell culture dish is ensured to be on the adjusted light path, and the cells are divided into a pseudo-radiation control group, a 6min group and a 60min group according to the irradiation time.
(C) Observing the morphology of the primary hippocampal neurites after terahertz wave radiation: at 1d, 2d and 3d after terahertz wave irradiation, the growth of the cell protrusions is observed by an IX70 inverted optical microscope (OLYMPUS, Japan) and 8 random visual fields in each group are photographed, and then the cell protrusions are traced by NeuronJ software, and the length and the number of branches are counted. The Sholl assay was used to evaluate cell growth. The Sholl analysis is a quantitative analysis method for characterizing the morphological characteristics of imaged neurons, and evaluates the morphological structure of the neurons by calculating the number of intersection points of concentric circles and branches with different radiuses from the center point of the cell body.
Under the light microscope, the cell body of the primary hippocampal neuron is in an oval shape, and bulges grow to the periphery, and continuously grow and prolong the bulges along with the prolonging of the culture time to generate a secondary branch, and compared with a control group, each radiation group has no obvious difference at each time point, which is shown in figure 2.
The total length of the primary hippocampal neuron processes and the number of the process branches are not obviously different at each time point, 6min and 60min radiation groups compared with a control group; no significant difference was seen in the 60min radiation group compared to the 6min radiation group, see tables 2 and 3.
Sholl analysis showed that no significant difference was seen at each time point for the primary hippocampal neurons in each irradiated group compared to the control group, see figure 3.
The results show that the 0.16THz/50mW terahertz wave has no obvious influence on the morphological structure of the hippocampal neuron.
TABLE 20.16 THz/50mW THz wave irradiation Total Length Change of Primary hippocampal neurites
TABLE 30.16 THz/50mW THz wave irradiation with changes in the number of primary hippocampal neurite outgrowths
(D) The neurotransmitter of the primary hippocampal neuron changes after terahertz wave radiation: immediately after terahertz wave irradiation, 300. mu.l of the cell culture medium was aspirated, centrifuged at 4 ℃ and 15000rpm for 20min using a 5804R low-temperature high-speed centrifuge (Eppendorf, Germany), and the supernatant was collected and stored in a refrigerator at-20 ℃. After an Agilent1100 high performance liquid chromatograph (Agilent, usa) is started to operate, the pressure is kept stable according to the sequence of methanol, water and a mobile phase. Standards for glycine, alanine, gamma aminobutyric acid, glutamic acid and cysteine at 0.01mg/ml, respectively, and mixed standards were tested. Every five samples were then tested, one mixed standard was tested. And after all samples are detected, flushing the system according to the sequence of water first and methanol second, quitting the chemical workstation, and closing the system.
As shown in Table 4, immediately after the irradiation with the terahertz wave, the glycine content (P < 0.01) was significantly increased in the 60min irradiated group compared with that in the group C, and no significant difference was observed in comparison among the groups, alanine, gamma aminobutyric acid, and glutamic acid. The terahertz wave of 0.16THz/50mW is shown to influence the transmitter metabolic activity of primary hippocampal neurons immediately after irradiation, and can cause the increase change of excitatory amino acid glycine.
TABLE 40.16 THz/50mW THz wave irradiation of Primary hippocampal neuronal neurotransmitter content
Note: in comparison with the group C,**show P<0.01。
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (10)
1. A terahertz wave vertical exposure system for cell experiments is characterized by comprising:
the terahertz wave source is placed with a light emitting port upwards so that the emitted terahertz waves are vertically upwards;
the converging lens is arranged above the light-emitting port so as to convert the terahertz waves into terahertz parallel waves;
the terahertz parallel wave focusing device comprises a terahertz wave source, a converging lens, a lifting table, a focusing lens and a focusing lens, wherein the terahertz wave source is arranged on the top of the terahertz wave source;
the bottom wall of the constant temperature box is provided with a second through hole which is sealed by a light-transmitting material, and the side wall of the constant temperature box is provided with a ventilation inlet and a ventilation outlet; the thermostat is arranged on the table top of the lifting table, and the second through hole is overlapped with the first through hole; a constant temperature heating plate, a thermometer and an air exhaust fan are arranged in the constant temperature box, and the air exhaust fan is connected with the air exchange inlet; the incubator is suitable for culturing cells, and a cell culture dish for culturing cells is placed above the second through hole;
the air exchange box is internally provided with an ultraviolet disinfection lamp, the side wall of the air exchange box is provided with an air inlet and an air outlet, the air inlet is connected with the air exchange outlet, and the air outlet is connected with the air exchange inlet;
the carbon dioxide gas cylinder is connected with the ventilation box;
the light emitting port of the terahertz wave source, the converging lens, the first through hole and the central axis of the second through hole are overlapped.
2. The vertical terahertz wave exposure system for cellular experiments according to claim 1, further comprising:
the terahertz wave source support is suitable for bearing the terahertz wave source, so that a light emitting opening of the terahertz wave source faces upwards.
3. The vertical terahertz wave exposure system for cell experiments according to claim 2, wherein the converging lens is supported by a liftable support.
4. The vertical terahertz wave exposure system for cell experiments according to claim 3, wherein the distance between the converging lens and the light emitting port of the terahertz wave source is the focal length of the converging lens.
5. The vertical terahertz wave exposure system for cell experiments according to claim 4, wherein the distance between the converging lens and the top surface of the lifting platform is 5-30 cm.
6. The vertical terahertz-wave exposure system for cell experiments according to claim 5, wherein the distance between the converging lens and the top surface of the lifting table is 5 cm.
7. The vertical terahertz-wave exposure system for cellular experiments according to claim 1, wherein the ventilation inlet and the ventilation outlet are respectively provided on two opposite side walls of the incubator.
8. The vertical terahertz wave exposure system for cellular experiments according to claim 7, wherein the gas inlet and the ventilation outlet and the gas outlet and the ventilation inlet are connected through a PVC pipe or a rubber pipe.
9. The vertical terahertz-wave exposure system for cellular experiments according to claim 1, wherein the first through hole and the second through hole have diameters not smaller than a diameter of the condensing lens.
10. The vertical terahertz wave exposure system for cell experiments according to claim 1, wherein the terahertz light source has an output frequency of 96 gigahertz-2.1 terahertz and an output power of not more than 65 milliwatts.
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| CN109593652A (en) * | 2018-12-24 | 2019-04-09 | 中国人民解放军军事科学院军事医学研究院 | The vertical exposure system of THz wave for cell experiment |
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| CN109593652A (en) * | 2018-12-24 | 2019-04-09 | 中国人民解放军军事科学院军事医学研究院 | The vertical exposure system of THz wave for cell experiment |
| CN109593652B (en) * | 2018-12-24 | 2023-09-26 | 中国人民解放军军事科学院军事医学研究院 | Terahertz wave vertical exposure system for cell experiment |
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