CN210062023U - Multichannel 3D prints shower nozzle, cell/biological ink conveying system - Google Patents
Multichannel 3D prints shower nozzle, cell/biological ink conveying system Download PDFInfo
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- CN210062023U CN210062023U CN201920371292.8U CN201920371292U CN210062023U CN 210062023 U CN210062023 U CN 210062023U CN 201920371292 U CN201920371292 U CN 201920371292U CN 210062023 U CN210062023 U CN 210062023U
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Abstract
The utility model provides a multichannel 3D prints shower nozzle, cell/biological ink conveying system belongs to 3D and prints technical field. The multi-channel 3D printing nozzle comprises a plurality of capillary tubes and an outer sleeve, wherein the capillary tubes are connected with each other through an adhesive and are arranged in the outer sleeve; the inner diameter of the capillary tube is 10-100 mu m independently, and the wall thickness of the capillary tube is 10-30 mu m independently. The multi-channel nozzle provided by the utility model has small capillary inner diameter and thin wall; any one or more of the 10 cell/bio-inks can be printed simultaneously; the printed cell/biological ink complex has the advantages of tight connection, good engagement and excellent biological activity.
Description
Technical Field
The utility model relates to a 3D prints technical field, especially relates to a multichannel 3D prints shower nozzle, cell/biological ink conveying system.
Background
Live cell 3D printing is a leading-edge technique to achieve regenerative reconstruction of human tissues and organs. At present, when living cell 3D prints preparation human class tissue and organoid, the shower nozzle that adopts is mostly single shower nozzle single channel structure. However, human tissues and organs are composed of various cells and various extracellular matrixes, and in order to construct tissues and organs more precisely by a living cell printing technology, a multi-channel nozzle capable of simultaneously printing various cells and various bio-inks is required. It is required that any one or more of cell and bio-ink can be printed by opening and closing different channels without time difference in channel switching.
SUMMERY OF THE UTILITY MODEL
In view of this, the present invention provides a multi-channel 3D printing head and a cell/bio-ink delivery system. The utility model provides a multichannel 3D prints shower nozzle can print multiple cell and biological ink simultaneously, and each other does not influence between different cell and the different biological ink, and the linkage is good, has excellent biological performance simultaneously.
In order to realize the purpose of the utility model, the utility model provides a following technical scheme:
the utility model provides a multi-channel 3D printing nozzle, which comprises a plurality of capillaries and an outer sleeve, wherein the capillaries are connected with each other through an adhesive and are arranged in the outer sleeve; the inner diameter of the capillary tube is 10-100 mu m independently, and the wall thickness of the capillary tube is 10-30 mu m independently.
Preferably, the diameter of the outer sleeve is 0.5-1.5 mm.
Preferably, the number of the capillaries is 2-10.
Preferably, the capillary tube and the outer sleeve are made of glass, ceramic, metal or high polymer.
The utility model also provides a based on above-mentioned technical scheme biological ink conveying system of shower nozzle is printed to multichannel 3D, including consecutive atmospheric pressure system, cell/biological ink container with multichannel 3D prints the shower nozzle, cell/biological ink container pass through the hose with multichannel 3D prints the capillary intercommunication of shower nozzle.
The utility model provides a multi-channel 3D printing nozzle, which comprises a plurality of capillaries and an outer sleeve, wherein the capillaries are connected with each other through an adhesive and are arranged in the outer sleeve; the inner diameter of the capillary tube is 10-100 mu m independently, and the wall thickness of the capillary tube is 10-30 mu m independently. The multi-channel 3D printing nozzle provided by the utility model has small inner diameter of the capillary tube and thin wall; the printed cell/biological ink has the advantages of tight connection, good engagement and excellent biological activity.
Drawings
Fig. 1 is a schematic structural view of a multi-channel 3D printing nozzle provided by the present invention;
fig. 2 is a schematic cross-sectional view of a multi-channel 3D printing nozzle provided by the present invention;
FIG. 3 is a schematic view of a cell/bio-ink delivery system provided by the present invention;
in fig. 3, 1 is a multi-channel 3D printing nozzle, 2 is a hose, 3 is a cell/bio-ink container, and 4 is an air pressure system.
Detailed Description
The utility model provides a multi-channel 3D printing nozzle, which comprises a plurality of capillaries and an outer sleeve, wherein the capillaries are connected with each other through an adhesive and are arranged in the outer sleeve; the inner diameter of the capillary tube is 10-100 mu m independently, and the wall thickness of the capillary tube is 10-30 mu m independently.
Fig. 1 is a schematic structural view of a multi-channel 3D printing nozzle provided by the present invention; is composed of multiple capillaries and an outer sleeve.
Fig. 2 is a schematic cross-sectional view of a multi-channel 3D printing nozzle provided by the present invention; where each circle represents a capillary glass tube and the dotted lines represent the outer sleeve.
The utility model discloses in, multichannel 3D prints the shower nozzle and includes many capillaries and an outer sleeve pipe, it is inside to be fixed in outer sleeve pipe through gluing agent connection between the capillary.
In the present invention, the inner diameter of the capillary is 10 to 100 μm, preferably 20 to 90 μm, and more preferably 30 to 80 μm. In the present invention, the wall thickness of the capillary is independently 10 to 30 μm, preferably 15 to 25 μm, and more preferably 20 μm. In the utility model discloses, the diameter of outer sheathed tube is preferred 0.5 ~ 1.5 mm. The utility model discloses it is right the quantity of capillary does not have special restriction, the field technique according to actual need select can, preferably include 2 ~ 10.
In the present invention, the material of the capillary tube and the outer sleeve is preferably glass, ceramic, metal or polymer. The utility model discloses do not have special restriction to the gluing agent kind between the capillary, adopt the gluing agent that technical personnel in the field are familiar with can, specifically, like 520 rapid-curing cutback glues.
The multi-channel 3D printing nozzle provided by the utility model has small inner diameter of the capillary tube and thin wall; the printed biological ink has the advantages of tight connection, good engagement and excellent biological activity.
The utility model also provides a cell/biological ink conveying system based on above-mentioned technical scheme multichannel 3D prints the shower nozzle, including consecutive atmospheric pressure system, cell/biological ink container with multichannel 3D prints the shower nozzle, cell/biological ink container pass through the hose with capillary intercommunication in the shower nozzle is printed to multichannel 3D.
FIG. 3 is a schematic view of a cell/bio-ink delivery system provided by the present invention; wherein, 1 is a multi-channel 3D printing nozzle, 2 is a hose, 3 is a biological/ink container, and 4 is an air pressure system; the cell/biological ink stored in the cell/biological ink container enters the multichannel 3D printing nozzle through the hose under the pushing of the air pressure system.
The utility model discloses it is not special restriction to the material of hose, adopt the medical hose that technical personnel in this field are familiar with can. The material of the cell/bio-ink container of the present invention is not particularly limited, and the cell/bio-ink container known to those skilled in the art may be used. The composition of the cell/bio-ink of the present invention is not specifically limited, and those skilled in the art can select the composition according to actual needs.
The utility model provides a cell/biological ink conveying system prints the shower nozzle based on multichannel 3D, can print multiple cell and biological ink simultaneously, and easy operation is convenient.
The following provides a detailed description of the multi-channel 3D printing head and the cell/bio-ink delivery system according to the embodiments, but they should not be construed as limiting the scope of the present invention.
Example 1
A multichannel 3D prints shower nozzle: the multichannel 3D printing nozzle comprises 5 capillary tubes and an outer layer sleeve, wherein the 5 capillary tubes are connected and fixed in the outer layer sleeve through an adhesive; the inner diameter of the capillary glass tube is 50 μm, and the wall thickness of the capillary tube is 20 μm; the diameter of the outer sleeve is 0.8 mm.
A cell/biomaterial conveying system based on the multi-channel 3D printing nozzle comprises an air pressure system, a cell/biomaterial container and the multi-channel 3D printing nozzle which are sequentially connected, wherein the cell/biomaterial container is communicated with a capillary tube in the multi-channel 3D printing nozzle through a hose.
Installing the cell/bio-ink delivery system of the embodiment into a 3D printer to print the cell/bio-ink; wherein the cell/bio-ink contained in the five cell/bio-material containers is respectively: cell 1: cardiomyocytes, bio-ink 1: a methacrylated gelatin solution, concentration 5 wt%; cell 2: fibroblast, bio-ink 2: a methacrylated gelatin solution, concentration 10 wt%; cell 3: vascular epithelial cells, bio-ink 3: methacrylated gelatin solution, concentration 15 wt%; cell 4: neural stem cells, bio-ink 4: methacrylated gelatin solution, concentration 20 wt%; cell 5: adipocytes, bio-ink 5: methacryloylated gelatin solution, concentration 25 wt%; the pressure of cell 1/bio-ink 1 is 30psi, the pressure of cell 2/bio-ink 2 is 35psi, the pressure of cell 3/bio-ink 3 is 40psi, the pressure of cell 4/bio-ink 4 is 45psi, and the pressure of cell 5/bio-ink 5 is 50 psi.
The printing parameters are as follows: the moving speed of a 3D printer nozzle is 300mm/min, the extrusion speed of the biological ink is 300mm/min, the wavelength of ultraviolet light is 315nm, and the illumination intensity of the ultraviolet light is 850mw/cm2. The compressive modulus of the hydrogel loaded with cells obtained is 120 kPa; after printing, the cell survival rate reaches 87%, and after 7 days of culture, the cell survival rate reaches 90%.
Example 2
A multichannel 3D prints shower nozzle: the multichannel 3D printing nozzle comprises 10 capillary tubes and an outer layer sleeve, wherein the 10 capillary tubes are connected and fixed in the outer layer sleeve through an adhesive; the inner diameter of the capillary tube is 50 μm, and the wall thickness of the capillary tube is 20 μm; the diameter of the outer sleeve is 0.8 mm.
A biological cell/biological ink conveying system based on the multi-channel 3D printing nozzle comprises an air pressure system, a cell/biological material container and the multi-channel 3D printing nozzle which are sequentially connected, wherein the cell/biological ink container is communicated with a capillary tube in the multi-channel 3D printing nozzle through a hose.
Installing the cell/bio-ink delivery system of the embodiment into a 3D printer to print the cell/bio-ink; cell 1: osteoblasts, bio-ink 1: sodium alginate solution with the concentration of 5 wt%; cell 2: fibroblast, bio-ink 2: sodium alginate solution with concentration of 4 wt%; cell 3: bone marrow stromal stem cell, bio-ink 3: sodium alginate, concentration 3 wt%; cell 4: chondrocytes, bio-ink 4: sodium alginate solution with the concentration of 3 wt%; cell 5: vascular epithelial cells, bio-ink 5: sodium alginate solution, concentration 2 wt%. Cell 6: smooth muscle cells, bio-ink 6: a methacrylated gelatin solution, concentration 5 wt%; cell: 7 osteoclast, bio-ink 7: a methacrylated gelatin solution, concentration 10 wt%; and (3) cell 8: columnar epithelial cells, bio-ink 8: methacrylated gelatin solution, concentration 15 wt%; cell 9: neural stem cells, bio-ink 9: methacrylated gelatin solution, concentration 20 wt%; cell 10: adipocytes, bio-ink 10: methacryloylated gelatin solution, concentration 25 wt%. The pressure of cell 1/bio-ink 1 is 50psi, the pressure of cell 2/bio-ink 2 is 40psi, the pressure of cell 3/bio-ink 3 is 35psi, the pressure of cell 4/bio-ink 4 is 30psi, the pressure of cell 5/bio-ink 5 is 25psi, the pressure of cell 6/bio-ink 6 is 30psi, the pressure of cell 7/bio-ink 7 is 35psi, the pressure of cell 8/bio-ink 8 is 40psi, the pressure of cell 9/bio-ink 9 is 45psi, and the pressure of cell 10/bio-ink 10 is 50 psi.
The printing parameters are as follows: the moving speed of a 3D printer nozzle is 350mm/min, the extrusion speed of the biological ink is 350mm/min, the wavelength of ultraviolet light is 365nm, and the illumination intensity of the ultraviolet light is 800mw/cm2. The compressive modulus of the hydrogel loaded with the cells is 130 kPa; after printing, the cell survival rate reaches 85%, and after 7 days of culture, the cell survival rate reaches 88%.
Example 3
A multichannel 3D prints shower nozzle: the multichannel 3D printing nozzle comprises 8 capillary tubes and an outer layer sleeve, wherein the 8 capillary tubes are connected and fixed in the outer layer sleeve through an adhesive; the inner diameter of the capillary tube is 80 μm, the wall thickness of the capillary tube is 30 μm, and the diameter of the outer sleeve is 1 mm.
A cell/biological ink conveying system based on the multi-channel 3D printing nozzle comprises an air pressure system, a cell/biological ink container and the multi-channel 3D printing nozzle which are sequentially connected, wherein the cell/biological ink container is communicated with a capillary glass tube in the multi-channel 3D printing nozzle through a hose.
Installing the cell/bio-ink delivery system of the embodiment into a 3D printer to print the cell/bio-ink; wherein the cell/bio-ink contained in the eight cell/bio-ink containers is respectively: cell 1: osteoblasts, bio-ink 1: hyaluronic acid solution, concentration 10 wt%; cell 2: fibroblast, bio-ink 2: hyaluronic acid solution, concentration 8 wt%; cell 3: bone marrow stromal stem cell, bio-ink 3: hyaluronic acid solution, concentration 7 wt%; cell 4: chondrocytes, bio-ink 4: hyaluronic acid solution with concentration of 6 wt%; cell 5: vascular epithelial cells, bio-ink 5: hyaluronic acid solution, concentration 5 wt%. Cell 6: smooth muscle cells, bio-ink 6: a methacrylated gelatin solution, concentration 5 wt%; and 7, cell: osteoclast, bio-ink 7: a methacrylated gelatin solution, concentration 10 wt%; and (3) cell 8: columnar epithelial cells, bio-ink 8: methacrylated gelatin solution, concentration 15 wt%; the pressure of cell 1/bio-ink 1 is 50psi, the pressure of cell 2/bio-ink 2 is 40psi, the pressure of cell 3/bio-ink 3 is 35psi, the pressure of cell 4/bio-ink 4 is 30psi, the pressure of cell 5/bio-ink 5 is 25psi, the pressure of cell 6/bio-ink 6 is 30psi, the pressure of cell 7/bio-ink 7 is 35psi, and the pressure of cell 8/bio-ink 8 is 40 psi.
The printing parameters are as follows: the moving speed of a 3D printer nozzle is 200mm/min, the extrusion speed of the biological ink is 200mm/min, the wavelength of ultraviolet light is 315nm, and the illumination intensity of the ultraviolet light is 900mw/cm2. The compressive modulus of the hydrogel loaded with the cells is 110 kPa; after printing, the cell survival rate reaches 88%, and after 7 days of culture, the cell survival rate reaches 92%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. The multichannel 3D printing nozzle is characterized by comprising a plurality of capillary tubes and an outer sleeve, wherein the capillary tubes are connected with each other through an adhesive and arranged in the outer sleeve; the inner diameter of the capillary tube is 10-100 mu m independently, and the wall thickness of the capillary tube is 10-30 mu m independently.
2. The multichannel 3D printing nozzle as claimed in claim 1, wherein the diameter of the outer sleeve is 0.5-1.5 mm.
3. The multi-channel 3D printing nozzle according to claim 1, wherein the number of the capillary tubes is 2-10.
4. The multi-channel 3D printing nozzle as claimed in claim 1, wherein the capillary tube and the outer sleeve are made of glass, ceramic, metal or polymer.
5. A cell/bio-ink delivery system based on the multi-channel 3D printing nozzle as claimed in any one of claims 1 to 4, comprising an air pressure system, a cell/bio-ink container and the multi-channel 3D printing nozzle as claimed in any one of claims 1 to 4, which are connected in sequence, wherein the cell/bio-ink container is communicated with a capillary tube of the multi-channel 3D printing nozzle through a hose.
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| CN201920371292.8U CN210062023U (en) | 2019-03-22 | 2019-03-22 | Multichannel 3D prints shower nozzle, cell/biological ink conveying system |
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| CN201920371292.8U CN210062023U (en) | 2019-03-22 | 2019-03-22 | Multichannel 3D prints shower nozzle, cell/biological ink conveying system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109822889A (en) * | 2019-03-22 | 2019-05-31 | 河北雄安大洲智造科技有限公司 | A kind of multichannel 3D printing spray head and preparation method thereof, cell/bio-ink transportation system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109822889A (en) * | 2019-03-22 | 2019-05-31 | 河北雄安大洲智造科技有限公司 | A kind of multichannel 3D printing spray head and preparation method thereof, cell/bio-ink transportation system |
| CN109822889B (en) * | 2019-03-22 | 2024-03-26 | 深圳大洲医学科技有限公司 | Multichannel 3D printing spray head, preparation method thereof and cell/biological ink conveying system |
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Effective date of registration: 20211213 Address after: No. 1301-76, sightseeing Road, Xinlan community, Guanlan street, Longhua District, Shenzhen City, Guangdong Province, 518109 Patentee after: Shenzhen Dazhou Medical Technology Co.,Ltd. Address before: 071000 3-1-501, community of land and Resources Bureau, No. 219, Yanling West Road, Anxin County, Baoding City, Hebei Province Patentee before: HEBEI XIONG'AN DAZHOU ZHIZAO TECHNOLOGY Co.,Ltd. |