CN210051623U - Spore stainer - Google Patents

Spore stainer Download PDF

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Publication number
CN210051623U
CN210051623U CN201920756533.0U CN201920756533U CN210051623U CN 210051623 U CN210051623 U CN 210051623U CN 201920756533 U CN201920756533 U CN 201920756533U CN 210051623 U CN210051623 U CN 210051623U
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CN
China
Prior art keywords
funnel
waste liquid
support frame
spore
dyeing
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201920756533.0U
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Chinese (zh)
Inventor
郝世苹
廖伟伟
黎兵
袁建波
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zunyi Medical University
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Zunyi Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Zunyi Medical University filed Critical Zunyi Medical University
Priority to CN201920756533.0U priority Critical patent/CN210051623U/en
Application granted granted Critical
Publication of CN210051623U publication Critical patent/CN210051623U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model provides a spore stainer, includes dyeing module and support frame, still including installing in the low-speed dye liquor funnel and washing the funnel at support frame top to and install in the waste liquid dish of support frame bottom, the dyeing module is used in the transition dish of dyeing fixed operation heat conduction and installs in the circulation slide frame on transition dish surface. The low-speed dye liquor funnel and the washing funnel are both located above the circulating slide rack, an alcohol lamp trough used for fixing an alcohol lamp is arranged in the middle of the base plate, and the alcohol lamp trough is located below the transition plate. The spore stainer has reasonable design structure, has the universality of bacterial staining, and can simultaneously perform staining operation treatment on a plurality of slides; the dyeing machine has the advantages of firm structure, corrosion resistance, easy cleaning and durability, and saves a large amount of tedious manual operation steps in the dyeing process. The spore stainer is convenient, clean and rapid as a whole, can clearly determine the using amount of dye solution, can ensure that the dye solution quickly drips out in the experimental process and is convenient to clean after dyeing is finished.

Description

Spore stainer
Technical Field
The utility model relates to an experiment in microorganism field detects utensil, concretely relates to is a spore stainer.
Background
In a microbiological laboratory, slide staining is required because morphological features of bacteria need to be observed under a microscope to determine the infection status of a patient. The operation process of the microorganism flaking and dyeing is very complicated, but the dyeing operation is the only method capable of determining diagnosis. In the whole operation process, the fixation and heating require time for drying, and the time of the fixation and heating requires manpower to hold the slide above the alcohol lamp for drying no matter how long the slide is, so that the operation is time-consuming and labor-consuming. If the manpower consumption in the heating link can be liberated, the time can be saved to do other experimental operations, and in the dyeing operation, the spore dyeing needs to be continuously dripped with the dye solution to prevent the dye solution from being excessively dried to influence the experimental result. In the process, the amount of the artificially dripped dye solution is more or less accurate enough, the spore dyeing is failed due to too little dye solution in the dyeing process, the spore dyeing is overlapped due to too much dye solution, the color is too thick, the observation under a lens is influenced, the detection efficiency is poor, and the condition of the patient cannot be diagnosed. Although the principle of spore staining is simple, the operation is complicated when a large number of slices are produced, and the error rate of operators is greatly increased. Laboratory contamination can be increased during manual transfer of slides, and the toxicity of the staining solution can also affect the health of the operator.
Therefore, aiming at the microorganism flaking and dyeing operation in a microorganism laboratory, a novel stainer which can maximally liberate manpower, is safe for operators, has less laboratory pollution, can dye in batches and is clean, time-saving and labor-saving is needed, and therefore, the spore stainer is specially designed.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a spore stainer to solve the problem that proposes among the above-mentioned background art. The spore stainer can simultaneously carry out dyeing operation treatment on a plurality of slides, simultaneously liberates a large number of tedious manual operation steps in the dyeing process, and is convenient to clean after dyeing is finished.
In order to achieve the above object, the utility model provides a following technical scheme: the dyeing machine comprises a dyeing module, a support frame, a low-speed dye solution funnel, a washing funnel and a waste liquid tray, wherein the low-speed dye solution funnel and the washing funnel are arranged at the top of the support frame, and the waste liquid tray is arranged at the bottom of the support frame.
The staining module comprises a transition disc for conducting heat in a staining fixing operation and a circulating slide rack mounted on the surface of the transition disc.
The support frame includes the horizontal pole that is used for fixed transition dish, is used for fixed low-speed dye liquor funnel and washes the mount table of funnel to and the chassis.
Be equipped with the half concave pipeline that can be used to the drainage waste liquid of slope and the reinforcement frame of vertical direction between chassis and the horizontal pole, the chassis four corners is equipped with the waste liquid hole of accepting with half concave pipeline adaptation, horizontal pole and mount table are through heightening a fixed connection.
The low-speed dye liquor funnel all is located directly over the circulation slide frame with washing the funnel, the chassis middle part is equipped with the alcohol lantern groove that is used for fixed alcohol lantern, just the alcohol lantern groove is located under the transition dish.
The circulating slide frame comprises a crawler belt, a transmission shaft capable of driving the crawler belt to rotate at a constant speed and slide screens uniformly distributed on the outer surface of the crawler belt, wherein an overflow groove penetrating through the crawler belt is arranged between the slide screens.
The low-speed dye liquor funnel comprises a dye liquor bucket cup, scale marks and a dye liquor nozzle, and the washing funnel comprises a water storage bucket cup and a washing nozzle.
The low-speed dye liquor funnel all is equipped with the velocity of flow switch with washing the funnel bottom, low-speed dye liquor funnel all is equipped with the fixture block with washing the funnel side, the mount table top is equipped with the reinforcement piece that can with the fixture block adaptation.
The support frame bottom is equipped with annular spike, just waste liquid dish passes through spike fixed connection with the support frame, the waste liquid dish is located waste liquid hole below, the waste liquid dish is including being located waste liquid dish all around protecting along, being located the handle of waste liquid dish both sides and being located the water conservancy diversion line of dish.
The semi-concave conduit is a groove-shaped conduit, the concave surface of the semi-concave conduit faces the outer side of the support frame, a guide hole penetrating through the wall of the conduit is formed in the part, close to the dyeing module, of the semi-concave conduit, and the dyeing module and the semi-concave conduit are connected with the guide hole in a buckling mode through a guide angle.
And a top baffle and a side baffle are respectively arranged at two ends of the slide clamping position, and a rotatable sealing plate fixed on the outer side of the crawler is arranged at the side end of the side baffle.
The transition dish both sides all are equipped with can with horizontal pole fixed connection's couple, the circulation slide frame outside is equipped with dead lever and the antiskid foot that is used for supporting.
The anti-skidding tooth with the inside face looks adaptation of track is all equipped with at the transmission shaft both ends, transmission shaft one end is equipped with micro motor, and the micro motor outside is equipped with waterproof shell, and the micro motor top is equipped with the switch.
Compared with the prior art, the beneficial effects of the utility model are that:
1. in the spore dyeing operation process, the spore stainer releases manpower in the steps of traditional manual dyeing, slide washing, heating for 20min and the like;
2. the circulating slide frame can fix a plurality of slides at the same time and drive the slides to circularly translate at a constant speed, so that the operation steps can be completed by comprehensively matching the low-speed dye solution funnel, the washing funnel and the alcohol lamp, and the problems of uneven dyeing, uneven heating degree and the like are solved;
3. the flow rate and the usage amount of the dye liquor can be conveniently controlled through the low-speed dye liquor funnel, the dye liquor is prevented from drying up, and the flow rate in the washing process can be conveniently controlled through the washing funnel;
4. the semi-concave pipeline structure can efficiently guide flow, so that the waste dye liquor is prevented from being crystallized and blocking a pipeline in the flowing process, and meanwhile, the dye liquor cylinder and the waste liquor tray can be detached and cleaned, and the cleaning work after the experiment is finished is facilitated.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of a partial structure of the supporting frame of the present invention;
FIG. 3 is a partial schematic structural view of a low-speed dye liquor funnel and a rinsing funnel of the present invention;
fig. 4 is a schematic view of a partial structure of the dyeing module of the present invention;
FIG. 5 is a schematic view of a partial structure of the circulating slide rack of the present invention;
fig. 6 is a schematic sectional structure view of the transmission shaft of the present invention;
FIG. 7 is a schematic view of a partial structure of the waste liquid tray of the present invention;
fig. 8 is a partial structural schematic view of the semi-concave catheter of the present invention;
in the figure: 1-a support frame; 101-a temple; 102-waste well; 103-alcohol lamp groove; 104-a chassis; 105-a semi-concave conduit; 106-reinforcing frame; 107-cross bar; 108-heightening frame; 109-mounting table; 110-reinforcement blocks; 2-a waste liquid tray; 201-protective edge; 202-flow guide lines; 203-a handle; 3-a dyeing module; 301-a baffle; 302-a transition disk; 303-diversion angle; 304-a hook; 4-low-speed dye liquor funnel; 401-dye liquor bucket cup; 402-a fixture block; 403-graduation mark; 404-a flow switch; 405-a staining fluid nozzle; 5-washing the funnel; 501-water storage bucket cup; 502-rinsing the nozzle; 6-circulating the slide rack; 601-track; 602-a fixation rod; 603-antiskid feet; 604-a drive shaft; 605-a micro motor; 606-a switch; 607-an overflow tank; 608-a top baffle; 609-clamping the slide; 610-side baffle; 611-closing the plate; 612-anti-slip teeth; 613-waterproof outer shell; 7-diversion holes.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "end", "front end", "rear end", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "mounted," "disposed/opened," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1 to 8, the present invention provides a technical solution: the utility model provides a spore stainer, includes dyeing module 3 and support frame 1, still including installing in the low-speed dye liquor funnel 4 and the washing funnel 5 at 1 top of support frame to and install in the waste liquid dish 2 of 1 bottom of support frame.
The support frame 1; comprises a supporting foot 101; a waste liquid hole 102; an alcohol lamp bath 103; a chassis 104; 105 a semi-concave conduit; a reinforcing frame 106; a cross bar 107; heightening frame 108; a mounting table 109; and a reinforcement block 110.
The support frame 1 comprises a cross bar 107 for fixing a transition disc 302, a mounting platform 109 for fixing a low-speed dye liquor funnel 4 and a rinsing funnel 5, and a base plate 104.
An inclined semi-concave pipeline 105 for draining waste liquid and a reinforcing frame 106 in the vertical direction are arranged between the base plate 104 and the cross rod 107, waste liquid holes 102 matched and connected with the semi-concave pipeline 105 are formed in four corners of the base plate 104, and the cross rod 107 is fixedly connected with the mounting platform 109 through a heightening frame 108.
An alcohol lamp groove 103 used for fixing an alcohol lamp is arranged in the middle of the base plate 104, and the alcohol lamp groove 103 is located right below the transition plate 302.
The waste liquid tray 2; comprises a guard edge 201; a flow guide pattern 202; a handle 203;
the dyeing module 3; comprises 301-a baffle; 302-a transition disk; 303-diversion angle; 304-a hook;
the staining module 3 includes a transition tray 302 for conducting heat during a staining fixing operation and a circulation slide rack 6 mounted on the surface of the transition tray 302.
The low-speed dye solution funnel 4; comprises a dyeing liquid funnel cup 401; a fixture block 402; scale marks 403; a flow switch 404; a staining solution nozzle 405.
The washing funnel 5; comprises a water storage bucket cup 501; a rinse nozzle 502;
the low-speed dye solution funnel 4 and the washing funnel 5 are both positioned right above the circulating slide frame 6.
The slide screens 609 are respectively provided with a top baffle 608 and a side baffle 610 at two ends, and a rotatable sealing plate 611 fixed on the outer side of the crawler 601 is arranged at the side end of the side baffle 610.
The transition disc 302 is provided with hooks 304 on both sides for fixedly connecting with the cross bar 107, and the outer side of the circulating slide rack 6 is provided with a fixing rod 602 and an anti-slip foot 603 for supporting.
Anti-skidding teeth 602 matched with the inner surface of the track 601 are arranged at two ends of the transmission shaft 604, a micro motor 613 is arranged at one end of the transmission shaft 604, a waterproof shell 605 is arranged on the outer side of the micro motor 613, and a switch 606 is arranged at the top of the micro motor 613.
The low-speed dye liquor funnel 4: including dyeing liquid fill cup 401, scale mark 403 and dyeing liquid mouth 405, wash funnel 5: including retaining fill cup 501 and washing mouth 502, low-speed dye liquor funnel 4 all is equipped with flow switch 404 with washing 5 bottom ends of funnel, low-speed dye liquor funnel 4 all is equipped with fixture block 402 with washing 5 side of funnel, mount table 109 top is equipped with the reinforcement piece 110 that can with fixture block 402 adaptation.
In particular, the graduation marks 403 facilitate reading the amount of dye solution used in the dyeing operation, and the flow switch 404 is similar to the structure of a cock at the bottom of the separating funnel, thereby facilitating simple and rapid adjustment of the funnel flow.
The circulating slide rack 6: the device comprises a crawler 601, a transmission shaft 604 capable of driving the crawler 601 to rotate at a constant speed, and slide screens 609 uniformly distributed on the outer surface of the crawler 601, wherein an overflow groove 607 penetrating through the crawler 601 is arranged between the slide screens 609;
specifically, in the dyeing and washing processes, the caterpillar band 601 drives the slide clamping positions 609 to rotate at a constant speed, so that slides on the surfaces of the slide clamping positions 609 can fully contact with the dye solution and the clear water, batch operation is completed, operation quality of each slide is guaranteed, and further the dye solution and the clear water quickly flow down from the overflow groove 607 after the slides are washed, and cleaning is facilitated.
The semi-concave guide pipe 105 is a groove-shaped guide groove, the concave surface of the semi-concave guide pipe 105 faces the outer side of the support frame 1, a guide hole 7 penetrating through the pipe wall is formed in the part, close to the dyeing module 3, of the semi-concave guide pipe 105, and the dyeing module 3 and the semi-concave guide pipe 105 are connected with the guide hole 7 in a buckling mode through a guide angle 303.
Specifically, in the dyeing and washing processes, the dye solution and the clear water wash the slide, flow into the transition disc 302, flow to the semi-concave duct 105 through the flow guide angle 303, and flow into the waste liquid disc 2 through the semi-concave duct 105.
Support frame 1 bottom is equipped with annular spike 101, just waste liquid dish 2 passes through spike 101 fixed connection with support frame 1, waste liquid dish 2 is located waste liquid hole 102 below, waste liquid dish 2 is located waste liquid dish 2 guard edge 201 all around, is located the handle 203 of waste liquid dish 2 both sides and is located the water conservancy diversion line 202 of twining.
After the experiment, can take waste liquid dish 2 out through handle 203 to empty wherein waste liquid, water conservancy diversion line 202 can prevent that the dye liquor from adhering to the crystallization, the clearance of being convenient for, half concave pipe 105 is the open pipe structure of flute shape, can prevent equally that the dye liquor from adhering to the crystallization and blockking up the pipe, the clearance of being convenient for is maintained.
The working principle is as follows: taking bacillus as an example, the staining operation comprises the steps of taking and placing a glass slide, baking, staining, washing and the like.
Placing and taking the glass slide: under the state that the circulating slide frame 6 is static or rotates at a constant speed, a slide is inserted into a slide clamping position 609 through one side of a side baffle 610, the tail end of the slide is attached to a top baffle 608, and a sealing plate 611 is rotated to seal the slide insertion end to reinforce the slide; similarly, when the slide is to be removed, the cover plate 611 is rotated and the slide is withdrawn from the end of the side stop 610.
Baking: install the slide in circulation slide frame 6 back, the manual fungus liquid coating operation that carries on, and accessible switch 606 starts circulation slide frame 6 and rotates at the uniform velocity, thereby carry out the fungus liquid coating operation of a plurality of slides, put into alcohol burner tank 103 with the alcohol burner afterwards, and ignite the alcohol burner, the transition dish 302 of dyeing module 3 bottom this moment will be heated by the alcohol burner, indirect baking heat circulation slide frame 6 bottom surfaces, thereby play the effect of toasting the slide, it can to extinguish the alcohol burner after the stoving finishes, and the accessible adds a small amount of clear water in transition dish 302 and carries out rapid cooling. The steps of fixing the bacillus and drying the dye liquor are the same.
Dyeing: under circulation slide frame 6 at the uniform velocity rotation state, install low-speed dye liquor funnel 4, add the dye liquor in the funnel to use flow switch 404 to adjust to suitable dye liquor velocity of flow, the rotation of circulation slide frame 6 this moment can make the slide uniform contact dye liquor of every slide screens 609, and it is abundant to dye.
Washing: the low-speed dye solution funnel 4 is taken down and replaced by a washing funnel 5, clear water is added into the funnel, the proper washing flow is adjusted through a flow switch 404, and at the stage, the circulating slide frame 6 rotates at a constant speed, so that slides in the slide clamping positions 609 can be fully washed.
The steps can be combined and used for different microorganism slice-making and dyeing operations.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. A spore stainer, which is characterized in that: the device comprises a dyeing module (3), a support frame (1), a low-speed dye solution funnel (4), a washing funnel (5), a circulating slide frame (6), a flow guide hole (7) and a waste liquid disc (2) which are arranged at the bottom of the support frame (1), wherein the low-speed dye solution funnel and the washing funnel are arranged at the top of the support frame (1);
the support frame (1); comprises a supporting foot (101); a waste liquid hole (102); an alcohol lamp groove (103); a chassis (104); a semi-concave conduit (105); a reinforcement frame (106); a cross bar (107); a heightening frame (108); a mounting table (109); a reinforcement block (110);
the support frame (1) comprises a cross rod (107) for fixing a transition disc (302), a mounting table (109) for fixing a low-speed dye liquor funnel (4) and a washing funnel (5), and a base plate (104);
an inclined semi-concave pipeline (105) which can be used for draining waste liquid and a reinforcing frame (106) in the vertical direction are arranged between the base plate (104) and the cross rod (107), waste liquid holes (102) which are matched and connected with the semi-concave pipeline (105) are formed in four corners of the base plate (104), and the cross rod (107) is fixedly connected with the mounting platform (109) through a heightening frame (108);
the staining module (3) comprises a transition disc (302) for conducting heat in a staining fixing operation and a circulating slide rack (6) mounted on the surface of the transition disc (302);
the low-speed dye liquor funnel (4) and the rinsing funnel (5) are both positioned right above the circulating slide frame (6), an alcohol lamp groove (103) for fixing an alcohol lamp is arranged in the middle of the base plate (104), and the alcohol lamp groove (103) is positioned right below the transition plate (302);
the circulating slide rack (6) comprises caterpillar tracks (601), transmission shafts (604) capable of driving the caterpillar tracks (601) to rotate at a constant speed and slide screens (609) uniformly distributed on the outer surfaces of the caterpillar tracks (601), wherein overflow grooves (607) penetrating through the caterpillar tracks (601) are arranged between the slide screens (609);
the low-speed dye liquor funnel (4) comprises a dye liquor bucket cup (401), scale marks (403) and a dye liquor nozzle (405), and the flushing funnel (5) comprises a water storage bucket cup (501) and a flushing nozzle (502);
the bottom ends of the low-speed dye liquor funnel (4) and the flushing funnel (5) are respectively provided with a flow speed switch (404), the side faces of the low-speed dye liquor funnel (4) and the flushing funnel (5) are respectively provided with a clamping block (402), and the top of the mounting table (109) is provided with a reinforcing block (110) which can be matched with the clamping block (402).
2. A spore stainer as claimed in claim 1, wherein: support frame (1) bottom is equipped with annular spike (101), just waste liquid dish (2) and support frame (1) are through spike (101) fixed connection, waste liquid dish (2) are located waste liquid hole (102) below, waste liquid dish (2) are including being located waste liquid dish (2) guard edge (201) all around, being located handle (203) of waste liquid dish (2) both sides and being located water conservancy diversion line (202) of twining.
3. A spore stainer as claimed in claim 1, wherein: the concave surface of the semi-concave pipeline (105) faces the outer side of the support frame (1), a part, close to the dyeing module (3), of the semi-concave pipeline (105) is provided with a flow guide hole (7) penetrating through the pipe wall, and the dyeing module (3) and the semi-concave pipeline (105) are connected with the flow guide hole (7) in a buckling mode through a flow guide angle (303).
4. A spore stainer as claimed in claim 1, wherein: the two ends of the slide clamping position (609) are respectively provided with a top baffle (608) and a side baffle (610), and the side end of the side baffle (610) is provided with a rotatable sealing plate (611) fixed on the outer side of the crawler (601).
5. A spore stainer as claimed in claim 1, wherein: transition dish (302) both sides all are equipped with couple (304) with horizontal pole (107) fixed connection, circulation slide rack (6) outside is equipped with dead lever (602) and anti-skidding foot (603) that are used for supporting.
6. A spore stainer as claimed in claim 1, wherein: both ends of the transmission shaft (604) are provided with fixing rods (602) matched with the inner surface of the track (601), one end of the transmission shaft (604) is provided with a micro motor (613), a waterproof shell (605) is arranged on the outer side of the micro motor (613), and the top of the micro motor (613) is provided with a switch (606).
CN201920756533.0U 2019-05-24 2019-05-24 Spore stainer Expired - Fee Related CN210051623U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201920756533.0U CN210051623U (en) 2019-05-24 2019-05-24 Spore stainer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201920756533.0U CN210051623U (en) 2019-05-24 2019-05-24 Spore stainer

Publications (1)

Publication Number Publication Date
CN210051623U true CN210051623U (en) 2020-02-11

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ID=69396437

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201920756533.0U Expired - Fee Related CN210051623U (en) 2019-05-24 2019-05-24 Spore stainer

Country Status (1)

Country Link
CN (1) CN210051623U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110031280A (en) * 2019-05-24 2019-07-19 遵义医科大学 A kind of spore staining device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110031280A (en) * 2019-05-24 2019-07-19 遵义医科大学 A kind of spore staining device
CN110031280B (en) * 2019-05-24 2024-02-27 遵义医科大学 Spore dyeing device

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Granted publication date: 20200211

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