CN210048789U - Artificial blood vessel endothelialization culture apparatus - Google Patents
Artificial blood vessel endothelialization culture apparatus Download PDFInfo
- Publication number
- CN210048789U CN210048789U CN201920436824.1U CN201920436824U CN210048789U CN 210048789 U CN210048789 U CN 210048789U CN 201920436824 U CN201920436824 U CN 201920436824U CN 210048789 U CN210048789 U CN 210048789U
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- artificial blood
- blood vessel
- wall
- dropper
- rotary drum
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Abstract
The utility model provides an artificial blood vessel endothelialization culture apparatus, relates to artificial blood vessel and prepares technical field, including erectting the crossbeam on the culture dish fixed two coaxial otic placodes that set up on the terminal surface under the crossbeam the otic placode internal rotation is pegged graft and is had the rotary drum, the rotary drum stretches into in the culture dish, artificial blood vessel places just artificial blood vessel's both ends detachable fixes at the both ends of rotary drum in the rotary drum, is equipped with the burette in artificial blood vessel, one side of terminal surface is provided with the dead lever under the crossbeam, the one end of burette is stretched out connect behind the rotary drum on the dead lever the pipe wall of burette is equipped with the drip hole of one row of equipartition at the bottom. The novel artificial blood vessel dripping device uniformly drips cell suspension onto the inner wall of the artificial blood vessel, and rotates the artificial blood vessel at a constant speed through mechanical operation, so that endothelial cells can be quickly and uniformly adhered to the inner wall of the artificial blood vessel.
Description
Technical Field
The utility model relates to an artificial blood vessel preparation technical field, concretely relates to artificial blood vessel endothelialization culture apparatus.
Background
The endothelialization of the inner wall of the artificial blood vessel is the premise of successful transplantation of the artificial blood vessel, and the endothelialization method is that cell suspension is uniformly dripped on the inner wall of the artificial blood vessel and then placed in a culture dish for culture, so that the inner wall of the artificial blood vessel is full of a layer of endothelial cells; the method is characterized in that endothelial cells automatically fall onto the inner wall of the artificial blood vessel for cultivation by the gravity of the endothelial cells, the artificial blood vessel needs to be rotated by artificial experience in the planting process, the endothelial cells in cell suspension are ensured to be uniformly distributed on the inner wall of the artificial blood vessel, the operation process can be operated only by experience, the planting efficiency is low, the planting density is uneven, in addition, the cell suspension needs to be uniformly distributed when the cell suspension is dripped, and the operation is difficult, time-consuming and labor-consuming when the cell suspension is dripped because the artificial blood vessel is slender.
Disclosure of Invention
In order to solve the problems of the prior art, the utility model provides an artificial blood vessel endothelialization culture device, which drips the cell suspension evenly on the inner wall of the artificial blood vessel, and rotates the artificial blood vessel at uniform speed through mechanized operation, so that endothelial cells can be adhered to the inner wall of the artificial blood vessel quickly and evenly.
The purpose of the utility model can be realized by the following technical proposal: the utility model provides an artificial blood vessel endothelialization culture apparatus, is including erectting the crossbeam on the culture dish fixed two coaxial otic placodes of setting on the terminal surface under the crossbeam the otic placode internal rotation is pegged graft and is had the rotary drum, the rotary drum stretches into in the culture dish, and artificial blood vessel places in the rotary drum and artificial blood vessel's both ends detachable fix the both ends at the rotary drum, be equipped with the burette in artificial blood vessel, one side of terminal surface is provided with the dead lever under the crossbeam, the one end of burette is stretched connect after the rotary drum on the dead lever, the pipe wall of burette is the hole that one row of equipartitions was equipped with to the lower extreme.
Preferably, the cross beam is provided with a driving assembly, the driving assembly comprises a first motor and a gear, the first motor is in power connection with the gear through transmission of a belt and a belt pulley, a circle of gear ring is fixedly arranged on the outer wall of the rotary drum, and the gear is in meshing transmission with the gear ring.
Preferably, the dropper is rotationally sealed on the fixed rod, a plurality of stirring blades are arranged on the tube wall of the dropper, a rotating wheel is arranged on the dropper, the rotating wheel is in power connection with the motor II on the cross beam, and the rotating wheel is located between the fixed rod and the rotary drum.
Preferably, the both ends of rotary drum all are equipped with the tang of step form, and the tang department is equipped with the external screw thread, and solid fixed ring is the ring form, and its terminal surface is equipped with the recess be equipped with the internal thread on the inner wall of recess, the recess is screwed fixedly through interior external screw thread after with the tang wall and artificial blood vessel wall chucking. The two ends of the artificial blood vessel can be fixedly connected with the two ends of the rotary drum by the fixing ring, and the fixing ring can be detached, so that the artificial blood vessel is convenient to take down.
Preferably, the outer side wall of the drum is provided with a ring of stop ring, and the stop ring is positioned on the side surface of one lug plate far away from the fixed rod. The stop ring serves for positioning the drum when it is inserted on the lug.
Preferably, the tail end of the dropper connected with one end of the fixed rod is fixedly connected with a hose. The cell suspension can be uniformly poured into the dropper through the hose, so that the cell suspension dripped from the dripping hole of the dropper has the same amount.
The utility model discloses an application principle:
1. before the endothelialization culture of the artificial blood vessel, sterilizing the culture dish and the corresponding device, simultaneously extracting the rotary drum from the ear plate, for example, if the fixed rod is arranged at the left side of the cross beam, extracting the rotary drum from the right side of the ear plate, inserting the artificial blood vessel into the rotary drum, and fixing the two ends of the artificial blood vessel by using the fixed rings;
2. inserting the rotary drum with the artificial blood vessel installed on the ear plate from the right side, simultaneously inserting the dropper into the artificial blood vessel, keeping the artificial blood vessel and the dropper from being contacted during insertion, slightly rotating the rotary drum to enable the gear ring to be meshed with the gear in the insertion process until the stop ring is stopped at the ear plate;
3. placing the cross beam on a culture dish, starting a motor I, enabling a gear ring to rotate in a meshed manner, driving the artificial blood vessel to rotate by the rotary drum, flushing cell suspension into the dropper from the hose, dripping the cell suspension onto the inner wall of the artificial blood vessel from a dripping hole at the lower end of the dropper, and injecting culture solution into the culture dish on the basis of submerging the upper end of the rotary drum, wherein the dropped cell suspension can be uniformly distributed on the inner wall of the artificial blood vessel because the artificial blood vessel rotates at a constant speed;
4. when the cell suspension is uniformly distributed on the inner wall of the artificial blood vessel, the first motor stops rotating, the second motor rotates to drive the dropper to rotate, the stirring blades on the dropper stir the cell suspension in the artificial blood vessel, more endothelial cells contained in the cell suspension can be adhered to the inner wall of the artificial blood vessel under the action of spiral centrifugation, more dense endothelial cells are uniformly distributed on the inner wall of the artificial blood vessel, and the culture efficiency of endothelialization is accelerated.
The utility model discloses the combined effect who brings includes: the utility model has simple and reasonable structure and convenient use, the drip tube is provided with a row of drip holes which are evenly distributed, the drip holes are inserted into the artificial blood vessel, and the artificial blood vessel rotates along with the rotary drum, so the cell suspension can evenly drip on the inner wall of the artificial blood vessel; meanwhile, the dropper can rotate, and the stirring blades arranged on the tube wall can generate a rotating vortex in the artificial blood vessel, so that endothelial cells in the cell suspension dropped on the inner wall of the artificial blood vessel can be more uniformly and densely adhered to the inner wall, and the efficiency of endothelialization culture is greatly improved; in addition, endothelial cells are planted through the uniform distribution of the drip holes of the dropper, so that the complexity, time and labor consumption in manual operation are avoided.
Drawings
FIG. 1 is a schematic structural view of an apparatus for culturing artificial blood vessel endothelialization according to an embodiment of the present invention.
FIG. 2 is a schematic view of the internal structure of the artificial blood vessel endothelialization culturing apparatus according to the embodiment of the present invention.
Wherein like parts are designated by like reference numerals throughout the several views; the figures are not drawn to scale.
Detailed Description
The invention is further described with reference to the following figures and examples.
Examples
The utility model provides an artificial blood vessel 4 endothelialization culture apparatus, is including erectting crossbeam 2 on culture dish 1 fixed two coaxial otic placodes 21 that set up on the terminal surface under crossbeam 2 the otic placode 21 internal rotation is pegged graft and is had rotary drum 3, rotary drum 3 stretches into downwards in the culture dish 1, and artificial blood vessel 4 places in the rotary drum 3 and artificial blood vessel 4's both ends detachable fixes the both ends at rotary drum 3, is equipped with burette 5 in artificial blood vessel 4, one side of terminal surface is provided with dead lever 22 under crossbeam 2, the one end of burette 5 is stretched out connect after rotary drum 3 on the dead lever 22 the pipe wall of burette 5 is equipped with the drip hole 51 of one row of equipartitions at the bottom. The cross beam 2 is provided with a driving assembly 6, the driving assembly 6 comprises a first motor 61 and a gear 62, the first motor 61 is in power connection with the gear 62 through transmission of a belt and a belt pulley, a circle of gear ring 63 is fixedly arranged on the outer wall of the rotary drum 3, and the gear 62 is in meshing transmission with the gear ring 63.
In this embodiment, the dropper 5 is rotationally sealed on the fixing rod 22, a plurality of stirring blades 52 are disposed on a tube wall of the dropper 5, a rotating wheel 7 is disposed on the dropper 5, the rotating wheel 7 is in power connection with a second motor 8 on the cross beam 2, and the rotating wheel 7 is located between the fixing rod 22 and the rotary drum 3. The end of the dropper 5 connected with the fixed rod 22 is fixedly connected with the hose 9. The cell suspension can be uniformly poured into the dropper 5 through the hose 9, so that the cell suspension dripped from the dripping hole 51 of the dropper 5 has the same amount.
In this embodiment, the both ends of rotary drum 3 all are equipped with the tang of step form, and tang department is equipped with the external screw thread, and solid fixed ring 10 is the ring form, and its terminal surface is equipped with the recess be equipped with the internal thread on the inner wall of recess, the recess is screwed fixedly through interior external screw thread after with tang wall and 4 wall chucking of artificial blood vessel. The two ends of the artificial blood vessel 4 and the two ends of the rotary drum 3 can be fixedly connected by the fixing ring 10, and the artificial blood vessel 4 can be conveniently taken down by the fixing ring 10 which can be disassembled. The outer side wall of the drum 3 is provided with a ring of stop ring 31, and the stop ring 31 is located on the side of one ear plate 21 far away from the fixed rod 22. The stop ring 31 serves for positioning when the drum 3 is inserted on the lug 21.
The utility model discloses use principle:
1. before the endothelialization culture of the artificial blood vessel 4, the culture dish 1 and the corresponding device are sterilized, and the rotary drum 3 is simultaneously pulled out from the ear plate 21, in the embodiment, the fixed rod 22 is arranged at the left side of the cross beam 2, then the rotary drum 3 is pulled out from the right side of the ear plate 21, the artificial blood vessel 4 is inserted into the rotary drum 3, and the two ends of the artificial blood vessel are fixed by the fixed rings 10;
2. inserting the rotary drum 3 with the artificial blood vessel 4 installed on the ear plate 21 from the right side, simultaneously inserting the dropper 5 in the artificial blood vessel 4, taking care to keep the artificial blood vessel 4 and the dropper 5 out of contact during the insertion, and slightly rotating the rotary drum 3 to enable the gear ring 63 to be meshed with the gear 62 during the insertion until the stop ring 31 is stopped at the ear plate 21;
3. placing the beam 2 on the culture dish 1, starting the motor I61, engaging and rotating the gear ring 63 of the gear 62, driving the artificial blood vessel 4 to rotate by the rotary drum 3, flushing cell suspension into the dropper 5 from the hose 9, dripping the cell suspension onto the inner wall of the artificial blood vessel 4 from the dripping hole 51 at the lower end of the dropper 5, and injecting culture solution into the culture dish 1 on the basis of submerging the upper end of the rotary drum 3, wherein the dropped cell suspension can be uniformly distributed on the inner wall of the artificial blood vessel 4 because the artificial blood vessel 4 rotates at a constant speed;
4. when the cell suspension is uniformly distributed on the inner wall of the artificial blood vessel 4, the first motor 61 stops rotating, the second motor 8 rotates, so that the dropper 5 is driven to rotate, the stirring blades 52 on the dropper 5 stir the cell suspension in the artificial blood vessel 4, more endothelial cells contained in the cell suspension can be adhered to the inner wall of the artificial blood vessel 4 under the action of spiral centrifugation, more dense endothelial cells are uniformly distributed on the inner wall of the artificial blood vessel 4, and the culture efficiency of endothelialization is accelerated.
The embodiment of the utility model provides a comprehensive effect brought includes: the embodiment of the utility model has simple and reasonable structure and convenient use, the dropper 5 is provided with a row of uniformly distributed dropping holes 51, the dropping holes 51 are inserted into the artificial blood vessel, and the artificial blood vessel rotates along with the rotary drum 3, so the cell suspension can be uniformly dropped on the inner wall of the artificial blood vessel; meanwhile, the dropper 5 can rotate, and the stirring blades 52 arranged on the tube wall can generate a rotating vortex in the artificial blood vessel, so that endothelial cells in the cell suspension dropped on the inner wall of the artificial blood vessel can be more uniformly and densely adhered to the inner wall, and the efficiency of endothelialization culture is greatly improved; in addition, endothelial cells are uniformly planted through the dripping holes 51 of the dropper 5, so that the complexity, time and labor are saved during manual operation.
The structure, ratio, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by people familiar with the technology, and are not used for limiting the limit conditions which can be implemented by the present invention, so that the present invention does not have the substantial significance in the technology, and any structure modification, ratio relationship change or size adjustment should still fall within the scope which can be covered by the technical content disclosed by the present invention without affecting the efficacy which can be produced by the present invention and the purpose which can be achieved by the present invention. Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for convenience of description, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof may be made without substantial technical changes, and the present invention is also regarded as the scope of the present invention.
The present invention has been described above with reference to the preferred embodiments, but the scope of protection of the present invention is not limited thereto, and all technical solutions falling within the scope of the claims are within the scope of protection of the present invention. Various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the technical features mentioned in the embodiments can be combined in any way as long as there is no structural conflict.
Claims (6)
1. The artificial blood vessel endothelialization culture device is characterized by comprising a cross beam erected on a culture dish, wherein two coaxial ear plates are fixedly arranged on the lower end face of the cross beam, rotary drums are inserted in the ear plates in a rotating mode, the rotary drums extend into the culture dish, artificial blood vessels are placed in the rotary drums, the two ends of the artificial blood vessels are detachably fixed at the two ends of the rotary drums, a dropper is arranged in each artificial blood vessel, a fixed rod is arranged on one side of the lower end face of the cross beam, one end of the dropper extends out of the rotary drums and then is connected to the fixed rod, and a row of uniformly distributed dropping holes are formed in the lowest end of the tube wall of the dropper.
2. The device for culturing artificial blood vessels for endothelialization according to claim 1, wherein a driving assembly is provided on the cross beam, the driving assembly comprises a first motor and a gear, the first motor is in power connection with the gear through the transmission of a belt and a belt pulley, a ring of gear rings are fixedly provided on the outer wall of the rotary drum, and the gear is in meshing transmission with the gear rings.
3. The apparatus according to claim 2, wherein the dropper is rotatably sealed on the fixing rod, the dropper has a plurality of stirring blades on the wall, the dropper has a rotating wheel, the rotating wheel is connected with the motor on the beam, and the rotating wheel is located between the fixing rod and the rotating drum.
4. The device for culturing the artificial blood vessel endothelialization according to claim 1, wherein step-shaped spigots are arranged at both ends of the rotary drum, external threads are arranged at the spigots, the fixing ring is annular, a groove is arranged at the end surface of the fixing ring, internal threads are arranged on the inner wall of the groove, the groove clamps the spigot wall and the artificial blood vessel wall and then screws and fixes the spigot wall and the artificial blood vessel wall through the internal threads and the external threads, the fixing ring can fixedly connect both ends of the artificial blood vessel and both ends of the rotary drum, and the fixing ring can be detached, so that the artificial blood vessel can be conveniently taken.
5. The device according to claim 1, wherein a stop ring is provided on the outer side wall of the drum, the stop ring being located on the side of one of the ear plates remote from the fixing rod.
6. The apparatus according to claim 2, wherein the dropper is connected to the fixing rod and the end of the dropper connected to the fixing rod is fixedly connected to a tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920436824.1U CN210048789U (en) | 2019-04-01 | 2019-04-01 | Artificial blood vessel endothelialization culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920436824.1U CN210048789U (en) | 2019-04-01 | 2019-04-01 | Artificial blood vessel endothelialization culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210048789U true CN210048789U (en) | 2020-02-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920436824.1U Expired - Fee Related CN210048789U (en) | 2019-04-01 | 2019-04-01 | Artificial blood vessel endothelialization culture apparatus |
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| Country | Link |
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| CN (1) | CN210048789U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115919503A (en) * | 2022-11-01 | 2023-04-07 | 首都医科大学附属北京朝阳医院 | Vascular endothelial cell elution auxiliary device and use method |
| CN116769598A (en) * | 2023-07-24 | 2023-09-19 | 柔脉医疗(深圳)有限公司 | In-vitro culture device for artificial tissue |
-
2019
- 2019-04-01 CN CN201920436824.1U patent/CN210048789U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115919503A (en) * | 2022-11-01 | 2023-04-07 | 首都医科大学附属北京朝阳医院 | Vascular endothelial cell elution auxiliary device and use method |
| CN115919503B (en) * | 2022-11-01 | 2024-03-22 | 首都医科大学附属北京朝阳医院 | Vascular endothelial cell elution auxiliary device and use method thereof |
| CN116769598A (en) * | 2023-07-24 | 2023-09-19 | 柔脉医疗(深圳)有限公司 | In-vitro culture device for artificial tissue |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200211 Termination date: 20210401 |
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| CF01 | Termination of patent right due to non-payment of annual fee |