CN210037620U - Micro-fluidic chip for detecting nitrogen and phosphorus content in water body - Google Patents
Micro-fluidic chip for detecting nitrogen and phosphorus content in water body Download PDFInfo
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- CN210037620U CN210037620U CN201920442331.9U CN201920442331U CN210037620U CN 210037620 U CN210037620 U CN 210037620U CN 201920442331 U CN201920442331 U CN 201920442331U CN 210037620 U CN210037620 U CN 210037620U
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Abstract
The utility model provides a detect nitrogen phosphorus content's micro-fluidic chip in water. The micro-fluidic chip comprises an upper layer, a diaphragm layer, a lower layer and a supporting layer which are sequentially arranged from top to bottom, wherein the upper layer, the diaphragm layer, the lower layer and the supporting layer are all circular; wherein: the upper layer is provided with an upper layer reagent injection port, N upper layer mixing cavities, N upper layer digestion cavities and N upper layer color development cavities; the lower layer is provided with a lower layer reagent injection port, N lower layer mixing cavities, N lower layer digestion cavities, N lower layer color development cavities and a reference pool; the upper reagent injection port overlaps with the upper reagent injection port in position and is separated by a membrane layer. The utility model provides a quick, convenient, the controllable preparation of different concentration digestion liquid can be realized to the micro-fluidic chip.
Description
Technical Field
The utility model relates to a detect nitrogen phosphorus content's micro-fluidic chip in water belongs to micro-fluidic chip technical field.
Background
A large amount of domestic sewage, farmland drainage or industrial wastewater is discharged into a water body, so that the content of nitrogen and phosphorus in the water is increased, a large amount of organisms or microorganisms are propagated, dissolved oxygen in the water is consumed, and the quality of the water body is deteriorated. In addition, the total nitrogen and total phosphorus content in different water sources such as lake water, reservoirs, aquaculture wastewater, seawater and the like is greatly different, and no standard detection method is available at present for nitrogen and phosphorus detection of various water bodies. The addition amount and the addition proportion of the potassium persulfate and the sodium hydroxide in the digestion solution relate to the digestion degree of a water sample and the accuracy of a test result, and the forms of nitrogen and phosphorus contained in water samples from different sources are different, so that the formulas of the digestion solution are different.
At present, the conventional operation is basically optimized moderately according to experience on the basis of national standards GB 11894-89 and GB11893-89, the variation range is small, the number of selected points is small, the representativeness is poor, and important information is easy to miss; in addition, each sample needs to be processed, detected and analyzed independently, and the workload is large. Therefore, the research and development of the micro-fluidic chip for detecting the content of nitrogen and phosphorus in the water body is of great significance.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, an object of the utility model is to provide a detect nitrogen phosphorus content's micro-fluidic chip in water utilizes this micro-fluidic chip can realize that the liquid that disappears batch, quick, accurate preparation and the water sample is batch, clear up, react fast.
In order to achieve the above purpose, the utility model provides a following technical scheme:
a micro-fluidic chip for detecting the content of nitrogen and phosphorus in a water body comprises an upper layer, a diaphragm layer, a lower layer and a supporting layer which are sequentially arranged from top to bottom, wherein the upper layer, the diaphragm layer, the lower layer and the supporting layer are all circular; wherein:
the upper layer is provided with an upper layer reagent injection port, N upper layer mixing cavities, N upper layer digestion cavities and N upper layer color development cavities, and the upper layer reagent injection port is provided with a corresponding upper layer liquid storage tank;
the lower layer is provided with a lower layer reagent injection port, N lower layer mixing cavities, N lower layer digestion cavities, N lower layer color development cavities and a reference pool, the lower layer reagent injection port is provided with a corresponding lower layer liquid storage pool, the lower layer mixing cavities, the lower layer digestion cavities and the lower layer color development cavities are distributed at equal intervals around the lower layer reagent injection port, the corresponding lower layer mixing cavities, the lower layer digestion cavities and the lower layer color development cavities are connected in series through equal-height and equal-length micro-channels and are connected to the lower layer reagent injection port, and the width of the micro-channels connecting the lower layer reagent injection port and the different lower layer mixing cavities is different; the reference pool is arranged at any blank position of the lower layer;
the upper reagent injection port overlaps and separates through the diaphragm layer with the position of lower floor's reagent injection port, and corresponding upper mixing chamber overlaps and separates through the diaphragm layer with the position of lower floor's mixing chamber, and corresponding upper strata is cleared up the chamber and is cleared up the position of chamber with the lower floor and overlap and separate through the diaphragm layer, and corresponding upper colour development chamber overlaps and separates through the diaphragm layer with the position of lower floor's colour development chamber.
Further, the microfluidic chip is divided into four layers, i.e., an upper layer (also referred to as a 1 st layer), a membrane layer (also referred to as a 2 nd layer), a lower layer (also referred to as a 3 rd layer), and a support layer (also referred to as a 4 th layer), wherein the upper layer and the lower layer are provided with respective microchannels, the membrane layer is used for separating respective portions of the upper layer and the lower layer, and the support layer is used for supporting the upper layer, the lower layer, and the membrane layer.
Further, the microfluidic chip may be prepared by: designing a chip by using drawing software, processing micro-channels and all functional units on a chip substrate by a micro-processing technology, then compounding the diaphragm between an upper layer and a lower layer, and then placing the compound on a supporting layer. The 4-layer substrate can be packaged by using the existing micromachining technology to assemble the microfluidic chip.
Further, each part of the microfluidic chip may be made of the same or different materials, and preferably, the material of the upper layer and the lower layer is one or a combination of two or more of polymethyl methacrylate (PMMA), Polycarbonate (PC), Polytetrafluoroethylene (PTFE), Polydimethylsiloxane (PDMS), glass, stainless steel, aluminum, and a silicon wafer.
Furthermore, in the micro-fluidic chip, the corresponding reagent injection port, the mixing cavity, the digestion cavity and the color development cavity of the upper layer and the lower layer respectively form a sample injection unit, a mixing unit, a digestion unit and a color development unit. In the use, the reagent injection mouth of upper strata is separated (bonding, sealed 1 st floor and 3 rd floor substrate) through the diaphragm layer in advance with the reagent injection mouth of lower floor, destroys the diaphragm through external force when needing to realize the intercommunication of two-layer reagent injection mouth, mixing chamber, digestion chamber, color development chamber are the same.
Further, the number of the mixing chambers, the digestion chambers, and the color development chambers included in the mixing unit, the digestion unit, and the color development unit in the microfluidic chip (i.e., the value of N) may be set as needed, and the value of N may be an integer greater than or equal to 2, preferably 8, 16, 24, 32, 64, or 128, but is not limited thereto.
Further, the microfluidic chip also comprises a transducer corresponding to each chamber of the lower layer and a corresponding control system; more preferably, the transducer is provided outside the support layer; more preferably, the transducer has a frequency of 20-500KHz and a power of 10-100W. The transducers are used to achieve mixing of the liquids of the different chambers. The transducer is used to generate ultrasonic waves, by which on the one hand fluid mixing and on the other hand chemical reactions are promoted, so long as it corresponds to each mixing or digestion or color development chamber of the lower layer. The control system is used for controlling the working power, time and the like of the transducer, needs to be designed independently and is placed outside the microfluidic chip.
Further, the mixing chamber, digestion chamber, and color development chamber connected together by the microchannel are called as corresponding mixing chamber, digestion chamber, and color development chamber, or a combination of the three.
Further, in the microfluidic chip, the corresponding upper mixing chamber, the upper digestion chamber and the upper color development chamber are arranged on the same radius of the upper layer.
Furthermore, in the micro-fluidic chip, the corresponding lower layer mixing cavity, the lower layer digestion cavity and the lower layer color development cavity are arranged on the same radius of the upper layer.
Furthermore, in the microfluidic chip, the upper mixing cavities, the upper digestion cavities and the upper color development cavities are distributed equidistantly around the upper reagent injection port, namely the straight-line distances between the adjacent upper mixing cavities are equal, the straight-line distances between the adjacent upper digestion cavities are equal, the straight-line distances between the adjacent upper color development cavities are equal, and the straight-line distances between each upper mixing cavity, each upper digestion cavity, each upper color development cavity and the upper reagent injection port are equal; that is, all the upper mixing chambers, the upper digestion chambers, and all the upper color development chambers are located on circumferences of different radii with the reagent injection port as the center, and are distributed at equal distances on the circumferences. Preferably, the micro-channels connected with the N upper digestion cavities form a circle, and the micro-channels connected with the N upper color development cavities form a circle.
Further, in the microfluidic chip, the lower layer mixing cavities, the lower layer digestion cavities and the lower layer color development cavities are distributed around the lower layer reagent injection port at equal intervals, namely that the straight line distances between the adjacent lower layer mixing cavities are equal, the straight line distances between the adjacent lower layer digestion cavities are equal, the straight line distances between the adjacent lower layer color development cavities are equal, and the straight line distances between each lower layer mixing cavity, each lower layer digestion cavity, each lower layer color development cavity and the lower layer reagent injection port are equal; that is, all the lower layer mixing chambers, the lower layer digestion chambers and all the lower layer color development chambers are respectively positioned on the circumferences with different radiuses and taking the reagent injection port as the center of a circle, and are distributed at equal distances on the circumferences.
In a microfluidic chip, the chambers may be provided with respective overflow ports for allowing excess liquid to flow out of the respective chambers. Preferably, each upper layer mixing cavity, each lower layer digestion cavity and each lower layer color development cavity are respectively provided with a corresponding overflow port; at least one overflow port is arranged on the micro-channel connected with the upper digestion cavity in series; at least one overflow port is arranged on the micro-channel which is connected with the upper color developing cavity in series.
In the microfluidic chip, the microchannels above the upper layer have the same height and width, and preferably, the height of the microchannel above the upper layer is 100-.
In the microfluidic chip, the microchannels above the lower layer have the same height but have different widths, preferably, the height of the microchannel in the lower layer is 100-: the width of each microchannel connecting the lower layer reagent injection port and different lower layer mixing cavities is increased by 50-200 microns in steps according to the clockwise sequence.
In the microfluidic chip, the length of each microchannel can be set according to requirements, such as: in the upper layer, the length of the micro-channel connecting the upper layer reagent injection port with the upper layer mixing cavity is 2000 microns, the length of the micro-channel connecting each upper layer digestion cavity is 3.2 centimeters, and the length of the micro-channel connecting each upper layer color development cavity is 7.8 centimeters; among the lower floor, the length of the microchannel connecting the reagent injection port of the lower floor and the mixing chamber of the lower floor is 2 cm, the length of the microchannel connecting the mixing chamber of the lower floor and the digestion chamber of the lower floor is 2 cm, and the length of the microchannel connecting the digestion chamber of the lower floor and the color developing chamber of the lower floor is 2 cm.
The utility model provides a technical scheme has following beneficial effect:
(1) the utility model provides a quick, convenient, the controllable preparation of different concentration digestion liquid can be realized to the micro-fluidic chip.
(2) The utility model provides a micro-fluidic chip size is adjustable, channel structure can design, can change according to experimental conditions is nimble at any time.
(3) The micro-fluidic chip can be used for batch, rapid screening and optimization of experimental conditions, only a few minutes are needed for changing each traditional sample into a batch of samples for several hours, and the detection efficiency is greatly improved.
(4) The micro-fluidic chip reduces the use of chemical reagents, has simple detection process route, greatly reduces the detection cost, and realizes the effects of high efficiency, low energy consumption, low reagent consumption, low discharge and low pollution.
Drawings
Fig. 1 is a schematic diagram of the microfluidic chip of example 1.
FIG. 2 is a schematic diagram of an ultrasonic hybrid system control system.
FIG. 3 shows the results of the N, P content absorbance in water.
Detailed Description
In order to clearly understand the technical features, objects and advantages of the present invention, the following detailed description is given to the technical solution of the present invention, but the technical solution of the present invention is not limited to the limit of the implementable range of the present invention.
Example 1
The present embodiment provides a microfluidic chip, the structure of which is shown in fig. 1, and the microfluidic chip includes: the upper layer (the 1 st layer), the diaphragm layer (the 2 nd layer), the lower layer (the 3 rd layer), the supporting layer (the 4 th layer) and the like are all round; wherein:
the upper layer and the lower layer are respectively provided with 8 channels, namely 8 groups of mixing cavities, digestion cavities, color development cavities and corresponding micro-channels;
an upper layer reagent injection port 11 positioned at the center of a circle and a corresponding upper layer liquid storage tank are arranged in the upper layer; the 8 upper mixing chambers 14 are respectively connected with the upper reagent injection port 11 through equal-width equal-height equal-length microchannels 171 and are respectively provided with corresponding overflow ports 181, the linear distances between the adjacent upper mixing chambers 14 are equal, the 8 upper mixing chambers 14 are uniformly distributed on one circumference, and the included angle between the adjacent upper reagent injection port 11 and the microchannel of the upper mixing chamber 14 is 45 degrees; the 8 upper digestion cavities 15 are connected in series through equal-height equal-width equal-length microchannels 172, a water sample injection port 12 is arranged on the series microchannels 172, the series microchannels 172 and the 8 upper digestion cavities 15 form a closed circle, the 8 upper digestion cavities 15 are uniformly distributed on the circle at equal intervals, and overflow ports 182 are arranged in 1 upper digestion cavity 15; the 8 upper color development cavities 16 are connected in series through the equal-height and equal-width micro-channel 173, and the series micro-channel 173 is provided with a water sample color development liquid injection port 13, the series micro-channel 173 and the 8 upper color development cavities 16 form a closed circle, the 8 upper color development cavities 16 are uniformly distributed on the circle at equal intervals, wherein 1 upper color development cavity 16 is provided with an overflow port 183; the corresponding upper mixing cavity 14, the upper digestion cavity 15 and the upper color development cavity 16 are arranged on the same radius of a circle with the upper reagent injection port 11 as the center; each micro-channel of the upper layer has the width of 100 microns and the height of 100 microns; the length of the micro-channel 171 connecting the upper reagent injection port 11 and the upper mixing chamber 14 is 2 cm, the length of the micro-channel 172 connecting each upper digestion chamber 15 is 3.2 cm, and the length of the micro-channel 173 connecting each upper chromogenic chamber 16 is 7.8 cm;
the lower layer is provided with a lower layer reagent injection port 31 positioned at the center of a circle and a corresponding lower layer liquid storage tank; the 8 lower layer mixing cavities 34 are respectively connected with the lower layer reagent injection port 31 through equal-height equal-length unequal-width micro-channels 371, corresponding overflow ports 381 are respectively arranged, the linear distances between every two adjacent lower layer mixing cavities 34 are equal, the 8 lower layer mixing cavities 34 are uniformly distributed on one circumference, and the included angle between the adjacent micro-channels 371 for connecting the lower layer reagent injection port 31 and the lower layer mixing cavities 34 is 45 degrees; the 8 lower digestion cavities 35 are connected with the corresponding lower mixing cavities 34 through equal-height equal-width equal-length micro-channels 372, each lower digestion cavity 35 is provided with an overflow port 382, and the included angle between the adjacent micro-channels 372 connected with the lower digestion cavities 35 and the lower mixing cavities 34 is 45 degrees; the 8 lower-layer color developing cavities 36 are connected with the corresponding lower-layer digestion cavities 35 through equal-height, equal-width and equal-length micro channels 373, each lower-layer color developing cavity 36 is provided with an overflow port 383, and the included angle between the adjacent micro channels 373 which are connected with the lower-layer color developing cavities 36 and the lower-layer digestion cavities 35 is 45 degrees; the corresponding lower layer mixing cavity 34, the lower layer digestion cavity 35 and the lower layer color development cavity 36 are arranged on the same radius of a circle with the lower layer reagent injection port 31 as the center; the height of each micro-channel of the lower layer is 100 microns, the width of the micro-channel 371 connecting the reagent injection port 31 of the lower layer and different mixing cavities 34 of the lower layer is different, and the micro-channels are gradually increased by a grade difference of 50 microns from 50 microns to 400 microns in the clockwise direction; the width of the micro-channel connecting the lower digestion cavity 35 and the lower mixing cavity 34 and the width of the micro-channel connecting the lower color development cavity 36 and the lower digestion cavity 35 are both 100 micrometers; the length of the micro-channel 371 connecting the lower layer reagent injection port 31 and the lower layer mixing cavity 34 is 2 cm, the length of the micro-channel 372 connecting the lower layer mixing cavity 34 and the lower layer digestion cavity 35 is 2 cm, and the length of the micro-channel 373 connecting the lower layer digestion cavity 35 and the lower layer color development cavity 36 is 2 cm; the lower layer is also provided with a reference pool 39 which is arranged at any blank position;
the upper layer reagent injection port 11 is overlapped with the position of the lower layer reagent injection port 31 and is separated by a diaphragm layer, the corresponding upper layer mixing cavity 14 is overlapped with the position of the lower layer mixing cavity 34 and is separated by a diaphragm layer, the corresponding upper layer digestion cavity 15 is overlapped with the position of the lower layer digestion cavity 35 and is separated by a diaphragm layer, and the corresponding upper layer color development cavity 16 is overlapped with the position of the lower layer color development cavity 36 and is separated by a diaphragm layer;
the upper layer and the lower layer are obtained by carving the corresponding structures such as a cavity, an injection port, an overflow port and the like on the PMMA substrate by utilizing a laser carving technology; the membrane layer (2 nd layer) is double faced adhesive tape, the supporting layer (4 th layer) is PMMA board, as the backup pad, adopts the hot pressing mode to bond each layer, seals into the chip.
Corresponding transducers (as shown in figure 2) are fixed below the support layer at positions corresponding to the mixing cavity, the digestion cavity and the color development cavity, corresponding control circuits 410, 411 and 412 are attached to the transducers, and the transducers of each type of functional cavity are connected in series to promote the mixing of the solvents and the chemical reaction by controlling the working procedures of the transducers, including frequency, time and power.
The embodiment also provides a detection method for detecting the content of nitrogen and phosphorus in the water body by using the micro-fluidic chip, which comprises the following steps:
(1) filling sodium hydroxide: 2mL of sodium hydroxide solution with the mass concentration of 5% is added into the upper layer liquid storage tank from an upper layer reagent injection port 11, and a sealing cover is covered, so that the sodium hydroxide is injected into each upper layer mixing cavity 14 under the driving of centrifugal force (rotating speed of 50 r/min);
(2) pouring potassium persulfate: breaking the diaphragm between the upper layer reagent injection port 11 and the lower layer reagent injection port 31, injecting 2mL of potassium persulfate solution with the mass concentration of 5%, covering the sealing cover, and injecting the potassium persulfate into each lower layer mixing cavity 34 under the driving of centrifugal force (rotating speed of 50 rpm); because the micro-channels 371 connecting the lower reagent injection port 31 and the lower mixing chamber 34 have different sizes and are continuously changed in a step manner, under the same centrifugal force, the amount of potassium persulfate injected into the lower mixing chamber 34 is also changed in a step manner;
(3) rapid mixing of potassium persulfate and sodium hydroxide in various ratios: destroying the diaphragm between each pair of corresponding upper mixing cavity 14 and lower mixing cavity 34, starting the control circuit 410, adjusting the transducer (frequency 20kHz, power 5W) for 30s, and uniformly mixing potassium persulfate and sodium hydroxide to obtain digestion solution (alkaline potassium persulfate) with different proportions;
(4) pouring a water sample: injecting a water sample to be digested (nitrogen-phosphorus mixed standard solution, 1.0 mg of nitrogen and 0.08 mg of phosphorus in each liter of solution) into each upper layer digestion cavity 15 through the water sample injection port 12 by using an injector or an injection pump, and sealing the water sample injection port 12;
(5) mixing of water sample and digestion solution: injecting digestion solution into the corresponding lower digestion cavity 35 under the driving of centrifugal force (rotating speed of 100 rpm), destroying a diaphragm between each pair of upper digestion cavity 15 and lower digestion cavity 35, starting a control circuit 411 to adjust the frequency (frequency of 20kHz and power of 5W) of the transducer to carry out treatment for 30s, and enabling a water sample to be digested and the digestion solution to be in contact mixing;
(6) digestion: placing the micro-fluidic chip under an ultraviolet lamp for digestion treatment for 5 minutes to obtain a digestion solution;
(7) detecting total nitrogen: quantitatively detecting the total nitrogen content in the sample in the micro-fluidic chip subjected to digestion treatment by using an ultraviolet-visible spectrophotometer, and testing absorbance values at the wavelengths of 220nm and 275 nm;
(8) injecting an ammonium molybdate solution: injecting an ammonium molybdate solution into each upper color development cavity 16 through the color development liquid injection port 13, and sealing the color development liquid injection port 13;
(9) and (3) color development reaction: transferring the digestion solution to each lower layer color development cavity 36 by using centrifugal force (rotating speed of 150 rpm), destroying a diaphragm between each pair of upper layer color development cavity 16 and lower layer color development cavity 36, starting a control circuit 412 to adjust the frequency (frequency of 20kHz and power of 5W) of the transducer to carry out treatment for 30s, and fully mixing and reacting the ammonium molybdate solution and the digestion solution for 5 minutes;
(10) detecting total phosphorus: and (3) quantitatively detecting the total phosphorus content in the sample in the digested microfluidic chip by using an ultraviolet-visible spectrophotometer, and testing the absorbance value at 700 nm. The results of the measurement are shown in FIG. 3, in which the abscissa is "the width of the microchannel connecting the lower reagent injection port and the different lower mixing chambers". The results in figure 3 give the optimal ratio of digestion solution (ratio of sodium hydroxide and potassium perphosphate) for digesting water samples of different magnitude N, P. The determination of the content of N needs to be performed under an alkaline condition, the determination of the content of P needs to be performed under an acidic environment, and since N, P tests are adopted and are performed in series and continuously, the amount of sodium hydroxide must be well controlled, and cannot be too small or too large, otherwise, the accuracy of the test result is affected, and the requirement can be well met by adopting the step structure of the embodiment 1 to regulate and control the ratio of the digestion solution (i.e. the step ratio of potassium persulfate to sodium hydroxide). In combination with the results of N, P, the ratio of digestion solution obtained when the width of the channel 371 is 250 μm is the optimum ratio.
Example 2
The embodiment adopts the method for detecting the content of nitrogen and phosphorus in the water body by using the microfluidic chip provided in embodiment 1, and the difference lies in that: and (3) digesting for 15min under an ultraviolet lamp, testing the total nitrogen content, wherein the frequency of the transducer is 100kHz, the power is 20W and the processing time is 30s when testing the total phosphorus content, and the result is shown in figure 3. the digestion solution ratio obtained when the width of the channel 371 is 200 microns is the optimal ratio by integrating N, P detection results.
Example 3
The embodiment adopts the method for detecting the content of nitrogen and phosphorus in the water body by using the microfluidic chip provided in embodiment 1, and the difference lies in that: the nitrogen and phosphorus mixed standard solution contains 3.5 mg of nitrogen and 0.4 mg of phosphorus per liter of solution; digesting for 15min under an ultraviolet lamp, and testing the total nitrogen content; when the frequency of the transducer is 150 kHz when the total phosphorus content is tested, the power is 30W, the processing time is 30s, the optimal proportion or the optimal proportion range of the digestion solution under the concentration of the sample is determined, and as a result, the optimal proportion is obtained when the width of the channel 371 is 200 microns by integrating N, P detection results shown in figure 3.
Example 4
The embodiment adopts the method for detecting the content of nitrogen and phosphorus in the water body by using the microfluidic chip provided in embodiment 1, and the difference lies in that: the nitrogen and phosphorus mixed standard solution contains 0.1mg of nitrogen and 0.3mg of phosphorus per liter of solution; when the frequency of the transducer is 100kHz when the total phosphorus content is tested, the power is 30W, the processing time is 30s, the optimal proportion or the optimal proportion range of the digestion solution under the concentration of the sample is determined, and as a result, the optimal proportion is obtained when the width of the channel 371 is 200 microns by integrating N, P detection results, as shown in figure 3.
Example 5
In this embodiment, a method for detecting the content of nitrogen and phosphorus in a water body by using a microfluidic chip similar to the microfluidic chip provided in embodiment 1 is provided, except that: the designed chip is 3 channels, the width of a micro channel from a lower layer reagent injection port to a lower layer mixing cavity of a lower layer (layer 3) is 200 micrometers, the width of a connecting micro channel from the mixing cavity to a digestion cavity and from the digestion cavity to a color development cavity is 100 micrometers, and the height of the connecting micro channel is 100 micrometers; introducing a water sample with standard concentration (N concentration: 2.3 mg/L; P concentration: 0.3 mg/L); digesting for 15min under an ultraviolet lamp, and testing the total nitrogen content; when the total phosphorus content is tested, the frequency of the transducer is 150 kHz, the power is 20W, the processing time is 30s, the total nitrogen and phosphorus concentration of a water sample to be tested is tested, the average value of the test results of 3 times is calculated, and the results are shown in Table 1.
Example 5 was designed to test the stability of the chip, thus fixing the digestion solution ratio, designing three channels, and comparing the stability of the test results of 3 groups; the accuracy of the chip and the test method is proved by comparing with the test result of the traditional method.
Comparative example 1
The water samples used in example 5 were tested according to the methods of national standards GB 11894-89 and GB11893-89, and the nitrogen and phosphorus contents were measured, with the results shown in Table 1.
TABLE 1 Absorbance results for N, P content in Water
As can be seen from the results in table 1: the method for detecting by adopting the novel microfluidic chip has the same good testing accuracy and precision as the traditional method.
Claims (9)
1. A micro-fluidic chip for detecting the content of nitrogen and phosphorus in a water body comprises an upper layer, a diaphragm layer, a lower layer and a supporting layer which are sequentially arranged from top to bottom, and is characterized in that the upper layer, the diaphragm layer, the lower layer and the supporting layer are all circular; wherein:
the upper layer is provided with an upper layer reagent injection port, N upper layer mixing cavities, N upper layer digestion cavities and N upper layer color development cavities, and the upper layer reagent injection port is provided with a corresponding upper layer liquid storage tank;
the lower layer is provided with a lower layer reagent injection port, N lower layer mixing cavities, N lower layer digestion cavities, N lower layer color development cavities and a reference pool, the lower layer reagent injection port is provided with a corresponding lower layer liquid storage pool, the lower layer mixing cavities, the lower layer digestion cavities and the lower layer color development cavities are distributed at equal intervals around the lower layer reagent injection port, the corresponding lower layer mixing cavities, the lower layer digestion cavities and the lower layer color development cavities are connected in series through equal-height and equal-length micro-channels and are connected to the lower layer reagent injection port, and the width of the micro-channels connecting the lower layer reagent injection port and the different lower layer mixing cavities is different; the reference pool is arranged at any blank position of the lower layer;
the upper reagent injection port overlaps and separates through the diaphragm layer with the position of lower floor's reagent injection port, and corresponding upper mixing chamber overlaps and separates through the diaphragm layer with the position of lower floor's mixing chamber, and corresponding upper strata is cleared up the chamber and is cleared up the position of chamber with the lower floor and overlap and separate through the diaphragm layer, and corresponding upper colour development chamber overlaps and separates through the diaphragm layer with the position of lower floor's colour development chamber.
2. The microfluidic chip according to claim 1, wherein N is an integer greater than or equal to 2.
3. The microfluidic chip according to claim 1, further comprising a transducer and a corresponding control system corresponding to each chamber of the lower layer.
4. The microfluidic chip according to claim 1, wherein the respective upper mixing chamber, upper digestion chamber, and upper color development chamber are disposed on the same radius of the upper layer; and the corresponding lower layer mixing cavity, the lower layer digestion cavity and the lower layer color development cavity are arranged on the same radius of the lower layer.
5. The microfluidic chip according to claim 1, wherein the upper mixing chambers, the upper digestion chambers and the upper color development chambers are distributed equidistantly around the upper reagent injection port, which means that the linear distances between adjacent upper mixing chambers are equal, the linear distances between adjacent upper digestion chambers are equal, the linear distances between adjacent upper color development chambers are equal, and the linear distances between each upper mixing chamber, each upper digestion chamber and each upper color development chamber are equal to the linear distance between the upper reagent injection port;
lower floor's hybrid chamber, lower floor clear up the chamber, lower floor's colour development chamber centers on lower floor's reagent injection port equidistance distributes and indicates that adjacent lower floor's hybrid chamber linear distance each other equals, and adjacent lower floor clears up the linear distance each other of chamber and equals, and adjacent lower floor's colour development chamber linear distance each other equals, each lower floor's hybrid chamber, each lower floor clear up chamber, each lower floor the colour development chamber with the linear distance of lower floor's reagent injection port equals.
6. The microfluidic chip according to claim 1, wherein each of the upper layer mixing chamber, each of the lower layer digestion chamber and each of the lower layer color development chamber is provided with a corresponding overflow port; at least one overflow port is arranged on the micro-channel connected with the upper digestion cavity in series; at least one overflow port is arranged on the micro-channel which is connected with the upper color developing cavity in series.
7. The microfluidic chip according to claim 1, wherein the upper layer and the lower layer are made of one or a combination of two or more of polymethyl methacrylate, polycarbonate, polytetrafluoroethylene, polydimethylsiloxane, glass, stainless steel, aluminum and silicon wafer.
8. The microfluidic chip according to claim 1, wherein the height of the microchannel in the upper layer is 100-3000 microns, and the width thereof is 100-3000 microns.
9. The microfluidic chip according to claim 1, wherein the height of the microchannel in the lower layer is 100-3000 microns, the width thereof is 100-3000 microns, and the width of each microchannel connecting the reagent injection port in the lower layer and the mixing chambers in the different lower layers is increased by 50-200 microns.
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