CN210030732U - Circulating tumor cell enrichment device with high capture rate - Google Patents

Abstract

The utility model discloses a circulation tumor cells enrichment device of high capture rate, including adsorption plate and centrifugation bucket, the left end of adsorption plate is connected with the base, and the lower right corner of adsorption plate settles and have the test-tube rack, the internally mounted of test-tube rack has the centrifuging tube, and the bottom of test-tube rack is connected with the revolving stage, the centrifugation bucket settles in the inside of deciding the bucket, and the internally mounted of centrifugation bucket has the baffle, the below of baffle is connected with the mount, the motor is installed to the lower extreme of centrifugation bucket, the guiding gutter has been seted up to the inside of adsorption plate, and the right side of guiding gutter is connected with the absorption passageway, the magnetic stripe is installed to the inside intermediate position of absorption passageway, and the connecting pipe is installed to the right-hand member of. The circulating tumor cell enrichment device with the high capture rate is provided with the centrifugal barrel and the adsorption plate which are matched with each other, so that blood is firstly captured on the adsorption plate for the first time, then is centrifuged in the centrifugal barrel, the second capture is realized, and the capture rate of the whole device is effectively improved.

Description

Circulating tumor cell enrichment device with high capture rate

Technical Field

The utility model relates to the technical field of medical equipment, specifically be a circulating tumor cell enrichment device of high capture rate.

Background

Circulating tumor cells refer to the exfoliated tumor cells that have undergone blood circulation, and enriching refers to an operation step of collecting trace elements to be measured from a large amount of parent substances to a smaller volume, thereby increasing the content thereof to a level above the lower limit of measurement. Since trace substances having a content of less than 0.01% in a sample must be pre-enriched if their content is below the lower limit of the assay, enrichment devices are commonly used in the detection of circulating tumor cells.

At present, an enrichment device on the market is difficult to combine an adsorption method and a centrifugation method for use in the using process, secondary capture is inconvenient to carry out, and the problem of low capture rate of circulating tumor cells is caused.

Disclosure of Invention

An object of the utility model is to provide a circulation tumor cell enrichment device of high capture rate to at present, the enrichment device on the market is difficult to combine the use with adsorption method and centrifugation in the use, is not convenient for carry out the secondary and catches, leads to the lower problem of capture rate of circulation tumor cell in solving the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a circulation tumor cells enrichment device of high capture rate, includes adsorption plate and centrifugal bucket, the left end of adsorption plate is connected with the base, and the lower right corner of adsorption plate settles there is the test-tube rack, the internally mounted of test-tube rack has the centrifuging tube, and the bottom of test-tube rack is connected with the revolving stage, the right side of test-tube rack is installed and is decided the bucket, and decides the upper end threaded connection of bucket and have sealed lid, the centrifugal bucket settles in the inside of deciding the bucket, and the internally mounted of centrifugal bucket has the baffle, the below of baffle is connected with the mount, the motor is installed to the lower extreme of centrifugal bucket, and the output shaft and the centrifugal bucket fixed connection of motor, the guiding gutter has been seted up to the inside of adsorption plate, and the right side of guiding gutter is connected with the absorption passageway, the magnetic stripe is installed to the.

Preferably, the rotating rod is installed at the joint of the adsorption plate and the base, the adsorption plate forms a rotating structure through the rotating rod and the base, and the lower end of the rotating rod is in threaded connection with a fixing bolt which penetrates through the rotating rod.

Preferably, the rotating shaft is installed at the center of the lower end of the rotating platform, and the rotating shaft and the rotating platform are matched with each other to form a rotating structure.

Preferably, the diversion trench is communicated with the adsorption channel, and the adsorption channel is of a wave structure.

Preferably, the left end and the right end of the baffle are both fixed with clamping blocks, and a clamping structure is formed between the clamping blocks and the centrifugal barrel.

Preferably, the fixing frame is internally provided with a limiting groove, the limiting groove is uniformly distributed in the fixing frame at equal intervals, the inner surface of the limiting groove is connected with a silica gel pad, and the silica gel pad is of an elastic structure.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the circulating tumor cell enrichment device with the high capture rate is provided with the baffle, so that a centrifugal tube placed in the centrifugal barrel can be limited when the centrifugal barrel works, the centrifugal tube is prevented from being thrown out in the high-speed operation process, the safety performance of the whole device is improved, and the baffle is fixed in the centrifugal barrel through the clamping effect, so that the disassembly is convenient;

2. the rotating rod is arranged and connected between the adsorption plate and the base, so that the adsorption plate is driven to rotate through the rotating action of the rotating rod, the inclination angle of the adsorption plate is adjusted, the flow velocity of blood liquid on the adsorption plate is controlled, blood can be fully contacted with the magnetic stripe, the adsorption effect of the magnetic stripe is enhanced, the tumor cell capture rate of the whole device is favorably improved, the rotating shaft and the rotating table are arranged to rotate, so that the test tube rack is driven to rotate, and a worker can conveniently collect blood residual liquid on the adsorption plate by using a plurality of centrifuge tubes for unified storage;

3. set up the adsorption channel and be the ripples form, thereby prolong the mobile stroke of blood liquid in the adsorption channel, so that the internal tumor cell of blood liquid can be fully adsorbed by the magnetic stripe, thereby further improve the tumor cell capture rate of whole device, set up centrifugal bucket and adsorption plate and mutually support, thereby combine together adsorption method and centrifugation, make blood carry out the first capture on the adsorption plate earlier, again centrifuge the bucket is inside to be centrifuged, realize the second capture, thereby effectively improve the capture rate of whole device, whole enrichment device's practicality has been strengthened greatly.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic view of the top view structure of the adsorption plate and the test tube rack of the present invention;

fig. 3 is a schematic view of a part of an enlarged structure at a in fig. 1 according to the present invention.

In the figure: 1. an adsorption plate; 2. a base; 3. a test tube rack; 4. centrifuging the tube; 5. a turntable; 6. fixing a barrel; 7. a sealing cover; 8. a centrifuge bucket; 9. a baffle plate; 10. a fixed mount; 11. an electric motor; 12. a diversion trench; 13. an adsorption channel; 14. a magnetic strip; 15. a connecting pipe; 16. a rotating rod; 17. fixing the bolt; 18. a rotating shaft; 19. a clamping block; 20. a limiting groove; 21. a silica gel pad.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: a circulating tumor cell enrichment device with high capture rate comprises an adsorption plate 1, a base 2, a test tube rack 3, a centrifuge tube 4, a rotary table 5, a fixed barrel 6, a sealing cover 7, a centrifuge barrel 8, a baffle 9, a fixing frame 10, a motor 11, a diversion trench 12, an adsorption channel 13, a magnetic stripe 14, a connecting pipe 15, a rotating rod 16, a fixing bolt 17, a rotating shaft 18, a clamping block 19, a limiting groove 20 and a silica gel pad 21, wherein the left end of the adsorption plate 1 is connected with the base 2, the lower right corner of the adsorption plate 1 is provided with the test tube rack 3, the centrifuge tube 4 is arranged inside the test tube rack 3, the bottom end of the test tube rack 3 is connected with the rotary table 5, the right side of the test tube rack 3 is provided with the fixed barrel 6, the upper end of the fixed barrel 6 is in threaded connection with the sealing cover 7, the centrifuge barrel 8 is arranged inside the fixed barrel 6, the baffle 9 is arranged inside the centrifuge barrel 8, and motor 11's output shaft and 8 fixed connection of centrifugal bucket, guiding gutter 12 has been seted up to adsorption plate 1's inside, and the right side of guiding gutter 12 is connected with adsorbs passageway 13, magnetic stripe 14 is installed to the inside intermediate position of adsorbing passageway 13, and the right-hand member of adsorbing passageway 13 installs connecting pipe 15, guiding gutter 12 is linked together with adsorption passageway 13, and the structure of adsorbing passageway 13 is the wave form structure, it is the ripple form to set up to adsorb passageway 13, thereby prolong the flow stroke of blood liquid in adsorbing passageway 13, so that the tumor cell in the blood liquid can fully be adsorbed by magnetic stripe 14, thereby further improve the tumor cell capture rate of whole device.

The connecting part of the adsorption plate 1 and the base 2 is provided with a rotating rod 16, the adsorption plate 1 forms a rotating structure with the base 2 through the rotating rod 16, the lower end of the rotating rod 16 is connected with a fixing bolt 17 in a threaded manner, the fixing bolt 17 penetrates through the rotating rod 16, the rotating rod 16 is arranged between the adsorption plate 1 and the base 2 so as to drive the adsorption plate 1 to rotate through the rotating action of the rotating rod 16, and then the inclination angle of the adsorption plate 1 is adjusted, thereby controlling the blood liquid flow rate on the adsorption plate 1, leading the blood to be fully contacted with the magnetic stripe 14, enhancing the adsorption effect of the magnetic stripe 14, being beneficial to improving the tumor cell capture rate of the whole device, the rotating shaft 18 is arranged at the center position of the lower end of the rotating platform 5, the rotating shaft 18 and the rotating platform 5 are mutually matched to form a rotating structure, the rotating shaft 18 and the rotating platform 5 can rotate so as, make things convenient for the staff to utilize a plurality of centrifuging tubes 4 to collect the blood residual liquid on the adsorption plate 1 to unified storage, the later stage utilizes.

The left end and the right end of the baffle 9 are both fixed with a clamping block 19, a clamping structure is formed between the clamping block 19 and the centrifugal barrel 8, when the baffle 9 is arranged, when the centrifugal barrel 8 works, the centrifuge tube 4 arranged in the centrifuge barrel 8 is limited, the centrifuge tube 4 is prevented from being thrown out in the high-speed operation process, the safety performance of the whole device is improved, the baffle 9 is fixed inside the centrifugal barrel 8 through the clamping action, the disassembly is convenient, the fixing frame 10 is internally provided with a limit groove 20, the limit grooves 20 are evenly and equidistantly distributed in the fixing frame 10, the inner surface of the limit groove 20 is connected with a silica gel pad 21, the structure of the silica gel pad 21 is an elastic structure, the silica gel pad 21 is made of soft silicon rubber material and is tightly attached to the inner part of the limit groove 20, so as to fix centrifuging tube 4, be favorable to strengthening the fixed effect of mount 10, avoid centrifuging tube 4 to be thrown away.

The working principle is as follows: for the circulating tumor cell enrichment device with high capture rate, firstly, the inclination angle of the adsorption plate 1 is adjusted according to the amount of blood stock solution, the fixing bolt 17 is unscrewed, then the adsorption plate 1 is rotated, so that the adsorption plate 1 rotates around the rotating rod 16 relative to the base 2, the base 2 supports the whole device, the whole device is rotated to a proper inclination angle, the fixing bolt 17 is screwed for fixing, then the extracted blood stock solution is poured into the diversion trench 12, the tissue solution in the diversion trench 12 flows to the inside of the adsorption channel 13, as the magnetic strip 14 is composed of immunomagnetic beads carrying cell surface adhesion molecules EpCAM, the magnetic strip 14 adsorbs tumor cells in the blood stock solution, so that white blood cells are left, the adsorbed blood residual solution flows into the centrifuge tube 4 through the connecting tube 15 for storage, when one centrifuge tube 4 is full, the centrifuge tube 5 is rotated, the rotating table 5 and the rotating shaft 18 rotate to drive the test tube rack 3 to rotate, so that the position of the centrifuge tube 4 is changed, and a new centrifuge tube 4 is used for continuous storage.

Then rotate sealed lid 7, open and decide bucket 6, stimulate baffle 9 upwards earlier, baffle 9 has certain elasticity and can take place to warp, make the fixture block 19 at baffle 9 both ends break away from centrifuge tube 8, thereby pick up baffle 9, then will store centrifuge tube 4 inside inserting the spacing groove 20 of mount 10, make centrifuge tube 4 and silica gel pad 21 closely laminate, thereby fix centrifuge tube 4, then block baffle 9 inside centrifuge tube 8 again, close sealed lid 7, starter motor 11, motor 11 drives centrifuge tube 8 and is deciding the inside rotation of bucket 6, carry out high-speed centrifugation, make tumour cell and leucocyte take place the layering, thereby catch tumour cell, just so accomplish the use of the circulation tumour cell enrichment device of whole high capture rate.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. The utility model provides a circulating tumor cell enrichment device of high capture rate, includes adsorption plate (1) and centrifugation bucket (8), its characterized in that: the left end of the adsorption plate (1) is connected with a base (2), the lower right corner of the adsorption plate (1) is provided with a test tube rack (3), a centrifuge tube (4) is installed inside the test tube rack (3), the bottom end of the test tube rack (3) is connected with a rotary table (5), a fixed barrel (6) is installed on the right side of the test tube rack (3), the upper end of the fixed barrel (6) is in threaded connection with a sealing cover (7), a centrifuge barrel (8) is installed inside the fixed barrel (6), a baffle (9) is installed inside the centrifuge barrel (8), a fixing frame (10) is connected below the baffle (9), a motor (11) is installed at the lower end of the centrifuge barrel (8), the output shaft of the motor (11) is fixedly connected with the centrifuge barrel (8), a diversion trench (12) is formed inside the adsorption plate (1), and an adsorption channel (13) is connected on the right side of the diversion trench (12), magnetic stripe (14) are installed to the inside intermediate position of adsorbing passageway (13), and just the right-hand member of adsorbing passageway (13) installs connecting pipe (15).

2. The device of claim 1, wherein the device comprises: the utility model discloses a rotary table, including adsorption plate (1), base (2), rotation rod (16) are installed to the junction of adsorption plate (1) and base (2), and adsorption plate (1) constitutes revolution mechanic through between rotation rod (16) and base (2), the lower extreme threaded connection of rotation rod (16) has fixing bolt (17), and fixing bolt (17) run through in the inside of rotation rod (16).

3. The device of claim 1, wherein the device comprises: the rotating shaft (18) is installed at the center of the lower end of the rotary table (5), and the rotating shaft (18) and the rotary table (5) are matched with each other to form a rotating structure.

4. The device of claim 1, wherein the device comprises: the diversion trench (12) is communicated with the adsorption channel (13), and the adsorption channel (13) is of a wave structure.

5. The device of claim 1, wherein the device comprises: the left end and the right end of the baffle (9) are both fixed with clamping blocks (19), and a clamping structure is formed between the clamping blocks (19) and the centrifugal barrel (8).

6. The device of claim 1, wherein the device comprises: limiting groove (20) have been seted up to the inside of mount (10), and limiting groove (20) even equidistance distributes in the inside of mount (10), the internal surface of limiting groove (20) is connected with silica gel pad (21), and the structure of silica gel pad (21) is elastic structure.

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