CN210030517U - Cell disruption device for purifying animal serum protein - Google Patents
Cell disruption device for purifying animal serum protein Download PDFInfo
- Publication number
- CN210030517U CN210030517U CN201920130454.9U CN201920130454U CN210030517U CN 210030517 U CN210030517 U CN 210030517U CN 201920130454 U CN201920130454 U CN 201920130454U CN 210030517 U CN210030517 U CN 210030517U
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- crushing
- cell
- box body
- screw
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- 102000004506 Blood Proteins Human genes 0.000 title claims abstract description 11
- 108010017384 Blood Proteins Proteins 0.000 title claims abstract description 11
- 238000007789 sealing Methods 0.000 claims description 3
- 230000003028 elevating effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 25
- 230000000694 effects Effects 0.000 abstract description 12
- 210000000170 cell membrane Anatomy 0.000 abstract description 7
- 238000000746 purification Methods 0.000 abstract description 2
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 238000010030 laminating Methods 0.000 abstract 1
- 210000004102 animal cell Anatomy 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000001254 nonsecretory effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a cell disruption device is used in purification of animal serum protein, the power distribution box comprises a box body and a serial communication port, the box is the setting of hollow cuboid structure, the recess opening sets up downwards, be equipped with the horizontally lead screw in the recess, it has the screw through the thread bush on the lead screw, lateral wall around the screw with the inner wall laminating of recess, the broken motor rigid coupling is in on the screw, broken oar is ann to the bottom of cell crushing roller. The utility model has the advantages of reasonable design, it is easy and simple to handle, set up the cell crushing roller that has broken oar through the both sides at supersonic generator, cooperation lead screw and screw through setting up the roating seat, the electric jar that goes up and down, the cooperation telescopic link has solved broken inhomogeneous problem to and destroy the poor drawback of cell membrane effect, improved crushing effect, need not to prolong crushing time and repeated breakage many times, could improve crushing effect, practiced thrift broken cost when having improved crushing efficiency.
Description
Technical Field
The utility model relates to an animal cell breakage technical field especially relates to a cell breaker is used in purification of animal serum protein.
Background
Cell disruption refers to a technique of releasing cell contents including target product components by destroying cell membranes and cell walls by external force, and is the basis for separating and purifying non-secretory biochemical substances or products synthesized in cells. Although the animal cells do not have cell walls, the animal cells have cell membranes, and a certain cell breaking device is needed to break the cell membranes, so that the purpose of extracting products is achieved. However, the cell disruption device in the market at present has unreasonable structural design, has the problem of uneven breakage, and the drawback of bad cell membrane effect, in order to improve the disruption effect, needs to prolong the disruption time and repeat the disruption many times, can improve the disruption effect, has improved broken cost when having reduced crushing efficiency.
Disclosure of Invention
The utility model aims to solve the defects of the prior art and provides a cell breaking device for purifying animal serum protein.
The utility model discloses a realize above-mentioned purpose, adopt following technical scheme:
a cell breaking device for purifying animal serum protein comprises a box body and is characterized in that the box body is of a hollow cuboid structure, a box door is hinged to the side wall of the box body, a sealing rubber pad is arranged between the box door and the side wall of the box body, a driving base is fixedly connected to the center of the bottom in the box body, a vertical rotating motor is mounted at the center in the driving base, the top end of the rotating motor penetrates through the driving base and is fixedly connected with a horizontal rotating seat, a plurality of symmetrical balls of the rotating motor are arranged between the rotating seat and the driving base, annular rails matched with the balls are arranged on the lower surface of the rotating seat and the upper surface of the driving base, a lifting electric cylinder is vertically mounted at the center of the upper surface of the rotating seat, a mounting seat is fixedly connected to the top end of the lifting electric cylinder, and a plurality of symmetrical telescopic rods of the lifting electric cylinder are arranged between the mounting seat and the rotating seat, the bottom of the telescopic rod is fixedly connected to the upper surface of the rotating seat, the top end of the telescopic rod is fixedly connected with the mounting seat, a crushing container is arranged in the mounting seat, a horizontal groove is formed in the top end of the interior of the box body above the crushing container, the opening of the groove is downward, a horizontal lead screw is arranged in the groove, one end of the lead screw close to the box door is rotatably arranged on the inner wall of the groove, the other end of the lead screw penetrates through the side wall of the groove and is coaxially connected with a driving motor, the driving motor is arranged on the inner wall of the top end of the box body, a nut is sleeved on the lead screw through threads, the front side wall and the rear side wall of the nut are attached to the inner wall of the groove, an ultrasonic transducer is vertically arranged at the bottom of the nut, an ultrasonic generator is arranged at the bottom of the ultrasonic transducer, and vertical cell crushing rollers are, the top end of the cell crushing roller is coaxially provided with a vertical crushing motor, the crushing motor is fixedly connected to the screw nut, and the bottom of the cell crushing roller is provided with a crushing paddle.
The lifting electric cylinder is a servo electric cylinder.
The crushing container is of a hollow cylinder structure.
The box door is provided with a handle.
The utility model has the advantages that: the utility model has the advantages of reasonable design, it is easy and simple to handle, set up the cell crushing roller that has broken oar through the both sides at supersonic generator, cooperation lead screw and screw through setting up the roating seat, the electric jar that goes up and down, the cooperation telescopic link has solved broken inhomogeneous problem to and destroy the poor drawback of cell membrane effect, improved crushing effect, need not to prolong crushing time and repeated breakage many times, could improve crushing effect, practiced thrift broken cost when having improved crushing efficiency.
Drawings
Fig. 1 is a schematic structural view of the present invention;
in the figure: 1-a box body; 2-a drive base; 3-mounting a base; 4, a telescopic rod; 5-lifting an electric cylinder; 6-a rotating electrical machine; 7-a rotating seat; 8-a ball bearing; 9-crushing the container; 10-a drive motor; 11-a groove; 12-a lead screw; 13-a nut; 14-an ultrasonic transducer; 15-a cell disruption roller; 16-a crushing motor; 17-an ultrasonic generator; 18-a crushing paddle; 19-a box door;
the following detailed description will be made in conjunction with embodiments of the present invention with reference to the accompanying drawings.
Detailed Description
The invention will be further explained with reference to the following figures and examples:
as shown in figure 1, a cell disruption device for purifying animal serum protein comprises a box body 1, and is characterized in that the box body 1 is of a hollow cuboid structure, a box door 19 is hinged to the side wall of the box body 1, a sealing rubber pad is arranged between the box door 19 and the side wall of the box body 1, a driving base 2 is fixedly connected to the center of the bottom in the box body 1, a vertical rotating motor 6 is arranged at the center in the driving base 2, the top end of the rotating motor 6 penetrates through the driving base 2 and is fixedly connected with a horizontal rotating base 7, a plurality of balls 8 symmetrical to the rotating motor 6 are arranged between the rotating base 7 and the driving base 2, annular rails matched with the balls 8 are arranged on the lower surface of the rotating base 7 and the upper surface of the driving base 2, and a lifting electric cylinder 5 is vertically arranged at the center of the upper surface of the rotating base 7, the top end of the lifting electric cylinder 5 is fixedly connected with a mounting seat 3, a plurality of telescopic rods 4 which are symmetrical about the lifting electric cylinder 5 are arranged between the mounting seat 3 and the rotating seat 7, the bottom of each telescopic rod 4 is fixedly connected to the upper surface of the rotating seat 7, the top end of each telescopic rod 4 is fixedly connected with the mounting seat 3, a crushing container 9 is arranged in each mounting seat 3, a horizontal groove 11 is formed in the top end of the inner portion of the box body 1 above the crushing container 9, the opening of each groove 11 is downward arranged, a horizontal lead screw 12 is arranged in each groove 11, one end of each lead screw 12, which is close to the box door 19, is rotatably arranged on the inner wall of each groove 11, a driving motor 10 is coaxially connected to the side wall of each lead screw 12, which penetrates through the corresponding groove 11, the driving motor 10 is arranged on the inner wall of the top end of the box body 1, and a screw nut 13, the front side wall and the rear side wall of the screw 13 are attached to the inner wall of the groove 11, an ultrasonic transducer 14 is vertically arranged at the bottom of the screw 13, an ultrasonic generator 17 is arranged at the bottom of the ultrasonic transducer 14, vertical cell crushing rollers 15 are respectively arranged at the left end and the right end of the ultrasonic generator 17, a vertical crushing motor 16 is coaxially arranged at the top end of each cell crushing roller 15, the crushing motor 16 is fixedly connected to the screw 13, and crushing paddles 18 are arranged at the bottom of each cell crushing roller 15.
The lifting electric cylinder 5 is a servo electric cylinder.
The crushing container 9 is of a hollow cylindrical structure.
The door 19 is provided with a handle.
When the utility model is used, the cell liquid to be crushed is put into the crushing container 9, the box door 19 is closed, the lifting electric cylinder 5 drives the mounting seat 3 to be matched with the telescopic rod 4 and moves to a proper height, so that the ultrasonic generator 17 and the crushing paddle 18 are completely immersed into the cell liquid to be crushed, the rotating motor 6 drives the rotating seat 7 to be matched with the ball 8 to rotate, the driving motor 10 drives the screw 13 to move along the groove 11 through the screw 12, meanwhile, the crushing motor 16 drives the cell crushing roller 15 provided with the crushing paddle 18 to rotate, the ultrasonic transducer 14 and the ultrasonic generator 17 crush the cells in the crushing container 9, the problem of uneven crushing is solved, the defect of poor cell membrane effect is overcome, the crushing effect is improved, the crushing effect can be improved without prolonging the crushing time and repeatedly crushing for many times, the crushing cost is saved while the crushing efficiency is improved, the utility model has the advantages of reasonable design, it is easy and simple to handle.
The present invention has been described above with reference to the accompanying drawings, and it is obvious that the present invention is not limited by the above embodiments, and various improvements made by the method concept and technical solution of the present invention or directly applied to other occasions without improvement are all within the protection scope of the present invention.
Claims (4)
1. The cell disruption device for purifying the animal serum protein comprises a box body (1) and is characterized in that the box body (1) is of a hollow cuboid structure, a box door (19) is hinged to the side wall of the box body (1), a sealing rubber pad is arranged between the box door (19) and the side wall of the box body (1), a driving base (2) is fixedly connected to the center of the bottom in the box body (1), a vertical rotating motor (6) is mounted at the center in the driving base (2), a horizontal rotating seat (7) is fixedly connected to the driving base (2) and is penetrated by the top end of the rotating motor (6), a plurality of balls (8) symmetrical to the rotating motor (6) are arranged between the rotating seat (7) and the driving base (2), and annular rails matched with the balls (8) are arranged on the lower surface of the rotating seat (7) and the upper surface of the driving base (2), the center of the upper surface of the rotating seat (7) is vertically provided with a lifting electric cylinder (5), the top end of the lifting electric cylinder (5) is fixedly connected with a mounting seat (3), a plurality of telescopic rods (4) which are symmetrical about the lifting electric cylinder (5) are arranged between the mounting seat (3) and the rotating seat (7), the bottom of each telescopic rod (4) is fixedly connected with the upper surface of the rotating seat (7), the top end of each telescopic rod (4) is fixedly connected with the mounting seat (3), a crushing container (9) is arranged in each mounting seat (3), a horizontal groove (11) is formed in the top end of the interior of the box body (1) above the crushing container (9), the opening of each groove (11) is arranged downwards, a horizontal lead screw (12) is arranged in each groove (11), one end, which is close to the box door (19), of each lead screw (12) is rotatably arranged on the inner wall of each groove (11), the other end of the screw rod (12) is coaxially connected with a driving motor (10) through the side wall of the groove (11), the driving motor (10) is arranged on the inner wall of the top end of the box body (1), the screw rod (12) is sleeved with a nut (13) through threads, the front side wall and the rear side wall of the screw nut (13) are attached to the inner wall of the groove (11), an ultrasonic transducer (14) is vertically arranged at the bottom of the screw nut (13), an ultrasonic generator (17) is arranged at the bottom of the ultrasonic transducer (14), the left end and the right end of the ultrasonic generator (17) are respectively provided with a vertical cell crushing roller (15), the top end of the cell crushing roller (15) is coaxially provided with a vertical crushing motor (16), the crushing motor (16) is fixedly connected to the nut (13), and the bottom of the cell crushing roller (15) is provided with a crushing paddle (18).
2. The cell disruption device for purifying animal serum protein according to claim 1, wherein said electric elevating cylinder (5) is a servo cylinder.
3. The cell disruption device for purifying animal serum protein as claimed in claim 1, wherein said disruption container (9) has a hollow cylindrical structure.
4. The cell disruption device for purifying animal serum protein according to claim 1, wherein said box door (19) is provided with a handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920130454.9U CN210030517U (en) | 2019-01-25 | 2019-01-25 | Cell disruption device for purifying animal serum protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920130454.9U CN210030517U (en) | 2019-01-25 | 2019-01-25 | Cell disruption device for purifying animal serum protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210030517U true CN210030517U (en) | 2020-02-07 |
Family
ID=69352715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920130454.9U Expired - Fee Related CN210030517U (en) | 2019-01-25 | 2019-01-25 | Cell disruption device for purifying animal serum protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210030517U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181669A (en) * | 2022-09-09 | 2022-10-14 | 鲲鹏基因（北京）科技有限责任公司 | Breaking device for cell or virus lysis |
-
2019
- 2019-01-25 CN CN201920130454.9U patent/CN210030517U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115181669A (en) * | 2022-09-09 | 2022-10-14 | 鲲鹏基因（北京）科技有限责任公司 | Breaking device for cell or virus lysis |
| CN115181669B (en) * | 2022-09-09 | 2022-11-11 | 鲲鹏基因（北京）科技有限责任公司 | Breaking device for cell or virus lysis |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201127 Address after: 239400 north of balinghu road and west of Guanshan Avenue, Changcheng New District, Mingguang City, Chuzhou City, Anhui Province Patentee after: Anhui Kangyuan Biotechnology Co.,Ltd. Address before: 301800 Tianjin city Xin'an District Baodi Town New Road North (positive Street Road No. 6) Patentee before: TIANJIN KANGYUAN BIOTECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200207 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |