CN210021253U - Affinity chromatographic column for purifying recombinant protein - Google Patents

Abstract

The utility model discloses an affinity chromatography column for purifying recombinant protein, which comprises a chromatography column and a bottom plate, wherein a vertical rod is welded on the left side of the upper end of the bottom plate, a fixing box is sleeved on the upper middle side of the vertical rod, the upper and lower sides of the right end of the fixing box are connected with a fixing clamp through bolts, the fixing clamp is connected with the chromatography column, a tetrafluoro sand plate layer is sleeved inside the lower side of the chromatography column, the calibration device of the utility model consists of a calibration sleeve, a horizontal rod and a telescopic rod, the telescopic rod is lengthened and kept at the same height with a burette, the calibration sleeve connected with the horizontal rod on the telescopic rod is operated by moving a test tube box, the calibration sleeve is sleeved into the burette, a test tube on the test tube box is kept on the same central numerical value line with the burette through the calibration sleeve, the calibration device of the utility model has the beneficial effects that the, the time is saved, and the working efficiency is greatly improved.

Description

Affinity chromatographic column for purifying recombinant protein

Technical Field

The utility model belongs to the technical field of chemistry experiment device is relevant, concretely relates to affinity chromatography column for purification recombinant protein.

Background

The chromatographic column is a main body in the gel chromatography technology and generally adopts a glass tube or an organic glass tube. A chromatographic method for separating components of a sample mixture by their different partition coefficients in a stationary phase and a mobile phase.

The prior art has the following problems: need aim at the test tube to the chromatographic column delivery port before using the chromatographic column to experiment and collect the dropping liquid of outflow, nevertheless usually proofread through the naked eye, time-wasting still easy calibration error, greatly reduced work efficiency.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a purification is affinity chromatography post for recombinant protein to the dropping liquid that flows is collected to the chromatography post delivery port need be aimed at with the test tube before using the chromatography post to experiment that proposes in solving above-mentioned background art, nevertheless usually proofreaies through the naked eye, and the time-wasting is calibration error still easily, greatly reduced work efficiency problem.

In order to achieve the above object, the utility model provides a following technical scheme:

the utility model provides a purification is affinity chromatography post for recombinant protein, includes chromatography column and bottom plate, the welding of bottom plate upper end left side has the montant, the well upper side cover of montant has connect fixed box, both sides bolted connection has the fixation clamp about the right-hand member of fixed box, fixed clamp cover has connect the chromatography column, the upside of chromatography column is inlayed and is had the mill mouth, the funnel has been cup jointed to the upper end of mill mouth, the inside tetrafluoro sand sheet layer that has cup jointed of downside of chromatography column, the tetrafluoro piston has been cup jointed to the lower extreme of chromatography column, the upper end right side of bottom plate is provided with the test tube box, the left downside bolted connection that test tube box upper end left side is located the tetrafluoro piston has calibrating device, the test tube has been.

Preferably, the calibrating device comprises a calibrating sleeve, a cross rod and a telescopic rod, the cross rod is connected to the upper side of the right end of the telescopic rod through a bolt, and the other end of the cross rod is sleeved with the calibrating sleeve.

Preferably, the tetrafluoro sand plate layer is including fixed cover, ooff valve, piston pipe, outlet pipe and adjust knob, the piston pipe has been run through at the outlet pipe middle part, fixed cover has been cup jointed to the outlet pipe upper end, the right-hand member bolted connection of piston pipe has the ooff valve, the left end bolted connection of piston pipe has adjust knob.

Preferably, the test tube box comprises a test tube groove and a box body, and the right side of the upper end of the box body is provided with the test tube groove.

Preferably, the number of the fixing clamps is two, and the two fixing clamps are used for fixing the upper side and the lower side of the chromatographic column respectively.

Preferably, the calibration sleeve is dropper-crossed on the same numerical line with the center of the test tube.

Preferably, the box body vertically fixes the test tube by inserting the test tube into the test tube slot.

Preferably, the vertical rod fixes and suspends the chromatographic column through the fixing box and the fixing clamp.

Compared with the prior art, the utility model provides an affinity chromatography column for purification recombinant protein possesses following beneficial effect:

1. the utility model discloses calibrating device comprises calibration cover, horizontal pole and telescopic link, lengthens the telescopic link and keeps unified height with the burette, makes calibration cover telescope burette operating the calibration cover that the horizontal pole is connected above the telescopic link through removing the test tube box, and the test tube passes through on calibration cover and the burette keeps same central numerical line on the test tube box.

2. The utility model discloses calibrating device beneficial effect is through calibrating device with the test tube quick calibration and improved the accuracy greatly, makes the dropping liquid directly instil into the test tube in, has saved the time and has improved work efficiency greatly.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description, do not constitute a limitation of the invention, in which:

FIG. 1 is a schematic diagram of an affinity chromatography column for purifying recombinant proteins according to the present invention;

FIG. 2 is a schematic structural diagram of an affinity chromatography column calibration device for purifying recombinant proteins according to the present invention;

FIG. 3 is a schematic diagram of the structure of the tetrafluoro piston of the affinity chromatography column for purifying recombinant protein according to the present invention:

FIG. 4 is a schematic view of the structure of a test tube box of an affinity chromatography column for purifying recombinant proteins according to the present invention;

in the figure: 1. a vertical rod; 2. a funnel; 3. grinding the opening; 4. a fixing clip; 5. a chromatography column; 6. a polytetrafluoroethylene sand board layer; 7. a tetrafluoro piston; 8. a dropper; 9. a calibration device; 10. a test tube; 11. a test tube box; 12. a base plate; 13. a fixing box; 61. fixing a sleeve; 62. an on-off valve; 63. a piston tube; 64. a water outlet pipe; 65. adjusting a knob; 91. calibrating the sleeve; 92. a cross bar; 93. a telescopic rod; 111. a test tube groove; 112. and (5) a box body.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to FIGS. 1-4, the present invention provides a technical solution of affinity chromatography column for recombinant protein purification:

the utility model provides a purification is affinity chromatography post for recombinant protein, including chromatography post 5 and bottom plate 12, the welding of bottom plate 12 upper end left side has montant 1, montant 1 is through fixed box 13 and fixation clamp 4 with chromatography post 5 fixed unsettled, wherein chromatography post 5 is just conveniently carried out the chemistry experiment by fixed unsettled back, fixing box 13 has been cup jointed to the well upper side of montant 1, both sides bolted connection has fixation clamp 4 about fixing box 13's the right-hand member, fixation clamp 4 is provided with two altogether, and two fixation clamps 4 fix the upper and lower both sides of chromatography post 5 respectively, wherein two fixation clamps 4 press from both sides chromatography post 5 tightly can not drop, fixation clamp 4 has cup jointed chromatography post 5, the upside of chromatography post 5 is inlayed and has been ground mouthful 3, funnel 2 has been cup jointed to the upper end of grinding mouthful 3, the inside tetrafluoro sand sheet layer 6 that has cup.

An affinity chromatography column for purifying recombinant protein, which comprises a chromatography column 5, wherein the lower end of the chromatography column is sleeved with a tetrafluoro piston 7, a tetrafluoro sand plate layer 6 comprises a fixed sleeve 61, a switch valve 62, a piston pipe 63, a water outlet pipe 64 and an adjusting knob 65, the middle part of the water outlet pipe 64 penetrates through the piston pipe 63, the upper end of the water outlet pipe 64 is sleeved with the fixed sleeve 61, the right end of the piston pipe 63 is in bolted connection with the switch valve 62, the left end of the piston pipe 63 is in bolted connection with the adjusting knob 65, liquid flows into the water outlet pipe 64 from the fixed sleeve 61 and is blocked by the piston pipe 63, the switch valve 62 is rotated, the liquid flows out of the water outlet pipe 64 and enters a dropper 8, the right side of the upper end of a bottom plate 12 is provided with a test tube box 11, the test tube box 11 comprises a test tube groove 111 and a box body 112, the box body 112 is inserted into the test tube groove, in which the test tube 10 is put into the test tube slot 111 of the box body 112 and fixed.

The utility model provides a purification is affinity chromatography column for recombinant protein, the left side bolted connection who is located tetrafluoro piston 7 on the left side of 11 upper ends of test tube box has calibrating device 9, calibrating device 9 includes calibration cover 91, horizontal pole 92 and telescopic link 93, telescopic link 93 right-hand member upside bolted connection has horizontal pole 92, calibration cover 91 has been cup jointed to the other end of horizontal pole 92, calibration cover 91 is run through by burette 8 with test tube 10's center on same numerical line, wherein calibrate through calibration cover 91 and make liquid drip into test tube 10, wherein lengthen telescopic link 93 and drip tube 8 keep uniform height, calibration cover 91 that cross pole 92 connects makes calibration cover 91 telescope burette 8 above operating telescopic link 93 through removing test tube box 11, calibrating device 9's right side has cup jointed test tube 10.

The utility model discloses a theory of operation and use flow: after the utility model is installed, firstly, the chromatographic column 5 is fixedly suspended through the fixing clamp 4 connected with the fixing box 13 on the vertical rod 1, then the test tube box 11 is placed on the bottom plate 12 at the lower end of the chromatographic column 5, the test tube 10 is placed in the test tube groove 111 on the box body 112 and fixed, the calibration is started by using the calibration device 9, the telescopic rod 93 is lengthened and kept at the same height with the dropper 8, the calibration sleeve 91 connected with the cross rod 92 on the telescopic rod 93 is operated by moving the test tube box 11, the dropper 8 is sleeved with the calibration sleeve 91, the test tube 10 on the test tube box 11 is kept on the same central numerical line with the dropper 8 through the calibration sleeve 91, the initial test is completed after the calibration, the funnel 2 is installed in the grinding port 3 sleeved at the upper end of the chromatographic column 5, the material is poured into the chromatographic column 5 from the funnel 2 to generate chemical reaction and is separated and purified through the tetrafluoro sand plate layer 6, the liquid flows into the water outlet 64 from the fixing sleeve 61 through, by rotating the switch valve 62, the liquid flows out of the outlet pipe 64 into the dropper 8, and drops out of the dropper 8 into the test tube 10.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. An affinity chromatography column for purifying recombinant proteins, comprising a chromatography column (5) and a bottom plate (12), characterized in that: the welding of bottom plate (12) upper end left side has montant (1), the well upper side cover of montant (1) has cup jointed fixed box (13), both sides bolted connection has fixation clamp (4) about the right-hand member of fixed box (13), chromatography post (5) have been cup jointed in fixation clamp (4), the upside of chromatography post (5) is inlayed and is had ground (3), funnel (2) have been cup jointed to the upper end of ground (3), the inside tetrafluoro sand sheet layer (6) that has cup jointed of downside of chromatography post (5), tetrafluoro piston (7) have been cup jointed to the lower extreme of chromatography post (5), the upper end right side of bottom plate (12) is provided with test tube box (11), the left side downside bolted connection that test tube box (11) upper end left side is located tetrafluoro piston (7) has calibrating device (9), test tube (10) have been cup jointed on the right side of calibrating device (9).

2. The affinity chromatography column according to claim 1, wherein: the calibrating device (9) comprises a calibrating sleeve (91), a cross rod (92) and a telescopic rod (93), the upper side of the right end of the telescopic rod (93) is connected with the cross rod (92) through a bolt, and the other end of the cross rod (92) is sleeved with the calibrating sleeve (91).

3. The affinity chromatography column according to claim 1, wherein: tetrafluoro sand board layer (6) are including fixed cover (61), ooff valve (62), piston pipe (63), outlet pipe (64) and adjust knob (65), outlet pipe (64) middle part is run through piston pipe (63), fixed cover (61) have been cup jointed on outlet pipe (64), the right-hand member bolted connection of piston pipe (63) has ooff valve (62), the left end bolted connection of piston pipe (63) has adjust knob (65).

4. The affinity chromatography column according to claim 1, wherein: the test tube box (11) comprises a test tube groove (111) and a box body (112), wherein the test tube groove (111) is formed in the right side of the upper end of the box body (112).

5. The affinity chromatography column according to claim 1, wherein: the two fixing clamps (4) are arranged, and the two fixing clamps (4) are used for fixing the upper side and the lower side of the chromatographic column (5) respectively.

6. The affinity chromatography column according to claim 2, wherein: the calibration sleeve (91) is penetrated by a dropper (8) and is on the same numerical line with the center of the test tube (10).

7. The affinity chromatography column according to claim 4, wherein: the box body (112) is inserted into the test tube groove (111) through the test tube (10) to vertically fix the test tube (10).

8. The affinity chromatography column according to claim 1, wherein: the vertical rod (1) fixes and suspends the chromatographic column (5) through the fixing box (13) and the fixing clamp (4).

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