CN210001871U - kinds of amnion carrier culture dish - Google Patents

Abstract

The utility model discloses an amnion carrier culture dish, including setting up the annular brace table and the fixed lantern ring in the culture dish, brace table and culture dish concentric, be provided with 40 ~ 100um nylon screen cloth on brace table's mesa, the nylon screen cloth cover on the brace table, its all around thermosetting is in the side of brace table, culture dish and brace table between the clearance form the fixed slot, the fixed lantern ring install the fixed slot in the fixed slot, the utility model discloses can effectively overcome and use the amnion to cultivate cell or load biological gel in-process amnion difficult to fix as the carrier now, curl easily, float scheduling problem.

Description

kinds of amnion carrier culture dish

Technical Field

The utility model belongs to the technical field of medical auxiliary instrument, concretely relates to kinds of amnion carrier culture dishes.

Background

The amnion is the innermost layer of the placenta, is smooth, has no blood vessels, nerves and lymph, has elasticity of , and is about 0.02-0.5 mm thick, and is divided into five layers under an electron microscope, namely an epithelial layer, a basement membrane, a compact layer, a fibroblast layer and a sponge layer, wherein the basement membrane and a amniotic membrane matrix layer contain a large amount of different collagens, mainly comprise I, III, IV, V and VII collagens, fibronectin, laminin and the like, and the amnion can serve as a transplanted basement membrane to play a role of new healthy and appropriate matrixes to promote epithelialization.

At present, the effect of constructing a tissue engineering amniotic membrane compound by taking an amniotic membrane as a carrier and inoculating cells or loading biological gel on the amniotic membrane is greatly higher than that of the amniotic membrane which is used alone, but the fixation is difficult due to the characteristic of thin texture of the amniotic membrane per se according to literature reports, namely (1) the amniotic membrane is directly adhered to the bottom of a culture dish, (2) the amniotic membrane is adhered to a specially processed glass slide, but the two methods have the defects that the adhered amniotic membrane is easy to curl in the process of preparing the compound and causes preparation failure, the two to three layers of the amniotic membrane loaded with cells are basically not adhered, the bottom of the amniotic membrane is not contacted with a culture medium, the cell culture medium is arranged above and below the amniotic membrane, the growth of the cells is more facilitated, more experimental purposes can be realized, and the removal is inconvenient.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the above problem, kinds of amnion carrier culture dishes are provided, this culture dish is exclusively used in and uses the amnion as the cell culture or the load biological gel of carrier, can effectively overcome and currently use the amnion to be difficult to fix as carrier culture cell or load biological gel in-process amnion, curl easily, float the scheduling problem.

The technical scheme of the utility model is that amnion carrier culture dishes comprise an annular supporting table and a fixed lantern ring which are arranged in the culture dish, wherein the supporting table is concentric with the culture dish, a 40-100 um nylon screen is arranged on the table top of the supporting table, the nylon screen covers the supporting table, the periphery of the nylon screen is thermally fixed on the side surface of the supporting table, a fixed groove is formed in a gap between the culture dish and the supporting table, and the fixed lantern ring is arranged in the fixed groove.

, the support platform has a width of 3-5 mm and a height less than the outer wall of the culture dish, three elongated holes equivalent to of the circumference are arranged on the side surface of the support platform, and the elongated holes are connected with the bottom of the culture dish.

, the culture dish and the support platform are body type structures and the materials of the culture dish and the support platform are the same.

, the fixed lantern ring is a nested double-layer structure which can be vertically split and is made of biological rubber.

, the lower lantern ring of the fixed lantern ring is provided with a holding point convenient for the operation of the tweezers.

The utility model discloses the beneficial effect who produces is: when the amnion is used as a carrier to culture cells or load biological gel or culture a plurality of layers of amnions loaded with cells, the lower lantern ring of the fixed lantern ring is firstly fixed in the fixed groove, then the amnion is erected on the support table of the culture dish, and the edge of the amnion is fixed in the fixed groove at the edge of the culture dish through the upper lantern ring of the fixed lantern ring, so that the amnion carrier is prevented from being curled in the culture process; a nylon screen mesh of 40-100 um is arranged on the surface of the supporting table, and a culture medium is added before the amnion is laid, so that the culture medium is infiltrated on the upper and lower parts of the amnion, which is more favorable for cell growth; after the amnion is loaded with cells or biological gel, the amnion can be conveniently taken out, and the situation that the amnion substrate surface and the cell inoculation surface cannot be clearly distinguished due to the fact that the amnion is twisted after the amnion is taken out can be avoided.

Drawings

Fig. 1 is a schematic perspective view of the present invention;

fig. 2 is a top view of the present invention;

FIG. 3 is a schematic view of the structure of the retaining collar;

in the figure: 1-culture dish, 2-support table, 3-fixed groove, 4-fixed lantern ring, 41-upper lantern ring, 42-lower lantern ring, 5-clamping point and 6-nylon screen.

Detailed Description

The present invention will be described with reference to the accompanying drawings in step .

As shown in figures 1 and 2, the utility model discloses an kinds of amnion carrier culture dishes, can be used to the amnion as carrier culture cell or load biological gel or cultivate the amnion of multilayer load cell or gel the utility model discloses an annular brace table 2 and the fixed lantern ring 4 of setting in the culture dish, wherein brace table 2 is located the middle part of culture dish 1 and with culture dish 1 concentricity, brace table 2's width is 3mm ~ 5mm, highly is less than culture dish 1's outer wall height, and there is three 's rectangular shape hole that is equivalent to brace table girth quartering, and this hole bottom is with the culture dish coplanar, is convenient for the culture solution to flow in, the utility model provides a culture dish 1 is style structure with brace table 2, and both materials are the same, be provided with 40 ~ 100um nylon screen 6 on brace table 2's the mesa, this nylon screen 6 covers on whole brace table surface, and the nylon screen hangs down all around and heat-sets up in brace table 2's side, is used for keeping the level of whole mesa to form the fixed slot that is used for installing fixed lantern ring 4 by the clearance between brace table 2 and.

As shown in fig. 3, the utility model provides a fixed lantern ring adopts the preparation of biological rubber, and its structure is the nested formula bilayer structure of split from top to bottom for fix the amnion edge between upper lantern ring 41 and lower floor's lantern ring 42, play the fixed amnion of being convenient for and conveniently take out the amnion, prevent the amnion effect that curls and bond. In order to take out the fixed lantern ring 4 installed in the fixed groove 3 conveniently, the edge of the lower lantern ring 42 is provided with the clamping points 5 which protrude upwards and are convenient for clamping and dragging by tweezers, so that the fixed lantern ring 4 can be taken out more conveniently.

The utility model discloses can fixed diameter 3cm ~ 10 cm's amnion, when regard as the amnion carrier culture cell or load biological gel or the amnion of cultivateing multilayer load cell, the lower floor lantern ring 42 that will fix lantern ring 4 is fixed in fixed slot 3 earlier, then erect the amnion on the brace table 2 of culture dish, and fix amnion edge in fixed slot 3 at the culture dish edge through the upper lantern ring 41 in the fixed lantern ring 4, prevent that the amnion from taking place to curl in the culture process, as shown in figure 3, in this embodiment, fixed lantern ring 4 adopts the preparation of biological rubber, the fixed lantern ring 4 of biological rubber can make the amnion at load cell or biological gel in-process, keep flat, prevent that the amnion from curling. In the process of culturing the amniotic membrane, a cover is required to be covered on the culture dish so as to culture the amniotic membrane in a sterile environment. When the amnion needs to be taken out, the cover is opened, the clamping point 5 on the lower lantern ring 42 can be directly dragged to take out the fixed lantern ring 4, the amnion is fixed by the fixed lantern ring, the condition of load surface torsion is avoided, and the amnion is convenient to move and take out.

Claims (5)

1. The amnion carrier culture dish is characterized by comprising an annular supporting platform and a fixing lantern ring, wherein the annular supporting platform and the fixing lantern ring are arranged in the culture dish, the supporting platform and the culture dish are concentric, a 40-100-micrometer nylon screen is arranged on the table top of the supporting platform, the nylon screen covers the supporting platform, the periphery of the nylon screen is thermally fixed on the side face of the supporting platform, a fixing groove is formed in a gap between the culture dish and the supporting platform, and the fixing lantern ring is arranged in the fixing groove.
2. 2. The amniotic membrane carrier culture dish of claim 1, wherein the supporting platform has a width of 3 mm-5 mm and a height less than the height of the outer wall of the culture dish, and the supporting platform has three elongated holes corresponding to of a quarter of the circumference on the side and connected to the bottom of the culture dish.
3. 3. The kinds of amniotic membrane carrier culture dish of claim 1, wherein the culture dish and the supporting platform are body type structures and are made of the same material.
4. 4. The kinds of amniotic membrane carrier culture dish of claim 1, wherein the fixing collar is a nested double-layer structure that can be vertically separated and is made of biological rubber.
5. 5. The amniotic membrane carrier culture dish according to claim 1, wherein the lower layer of the fixed ring has a holding point for forceps.

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