CN209934138U

CN209934138U - Novel two aqueous phase extraction mechanisms

Info

Publication number: CN209934138U
Application number: CN201920554567.1U
Authority: CN
Inventors: 李颖
Original assignee: Chongqing Medical and Pharmaceutical College
Current assignee: Chongqing Medical and Pharmaceutical College
Priority date: 2019-04-23
Filing date: 2019-04-23
Publication date: 2020-01-14
Anticipated expiration: 2029-04-23

Abstract

The utility model discloses a novel double-water-phase extraction mechanism, which comprises a liquid tank for storing liquid to be separated, wherein the liquid tank is connected with the feed end of a cell crusher through a pipeline, the discharge end of the cell crusher is connected with a cooler, the cooler is connected with the feed channel of a mixer through a pipeline, a first storage tank for storing a first extractant is connected with the feed channel of a first mixer through a pipeline, the discharge channel of the first mixer is connected with a first centrifuge through a pipeline, the outlet of a buffer tank is connected with the feed channel of a second mixer through a pipeline, a second storage tank for storing a second extractant is connected with the feed channel of a second mixer through a pipeline, the discharge channel of the second mixer is connected with a second centrifuge through a pipeline, and in the whole extraction process, extraction liquid and the extractant can be ensured to be fully dissolved in water to form a multiphase system, the subsequent centrifugal extraction is facilitated, and the loss of a liquid phase caused by undissolved substances is avoided.

Description

Novel two aqueous phase extraction mechanisms

Technical Field

The utility model relates to an extraction technical field especially relates to a novel two aqueous phase extraction mechanisms.

Background

Extraction, also known as solvent extraction or liquid-liquid extraction, also known as extraction, is a unit operation that separates mixtures by utilizing the different solubilities of components in the system in a solvent. That is, the method is a method of transferring a solute substance from one solvent to another solvent by utilizing the difference in solubility or partition coefficient of the substance in two solvents which are not soluble (or slightly soluble) with each other. The method is widely applied to chemical, metallurgical, food and other industries and is generally applied to petroleum refining industry.

The existing extraction device, such as the utility model with application number 201320266517.6, discloses an extraction device, which comprises an extraction tower and a pre-extraction device, wherein a water outlet of a screen box in the extraction tower is connected with a water inlet of the pre-extraction device; the device also comprises a slice separator, wherein a water outlet I of the slice separator is connected with a water inlet of the pre-extraction device, and a water outlet II of the slice separator is connected with a water inlet of the extraction tower; the pre-extraction device comprises a pre-extraction device upper part, a pre-extraction device connecting part and a pre-extraction device bottom, wherein the pre-extraction device upper part is connected with the pre-extraction device connecting part, and the pre-extraction device connecting part is connected with the pre-extraction device bottom. Aiming at the extraction of organic enzyme, protein, enzyme nucleic acid, thalli, cells, organelles, amino acid and antibiotic lamp biomolecular substances, the device can be used for realizing multi-stage extraction by arranging a plurality of or not arranging a device which can change the volume of the pre-extraction device through the connection part of the pre-extraction device, but because a device for effectively mixing extraction liquid and an extracting agent is lacked in the extraction process, if a multi-stage extraction mode is adopted, the extraction efficiency is not high, if only a one-stage pre-extraction mode is adopted, the extraction liquid and the extracting agent are not effectively mixed, the extraction quality is not high, and the device is particularly not suitable for the extraction of biomolecular substances.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to overcome the not enough of prior art existence, provide a novel two aqueous phase extraction mechanism.

In order to solve the technical problem, the utility model discloses a following technical scheme:

a novel double-aqueous-phase extraction mechanism comprises a liquid tank for storing liquid to be separated, wherein the liquid tank is connected with a feed end of a cell crusher through a pipeline, a discharge end of the cell crusher is connected with a cooler, the cooler is connected with a feed channel of a first mixer through a pipeline, a first storage tank for storing a first extraction agent is connected with a feed channel of the first mixer through a pipeline, the discharge channel of the first mixer is connected with a first centrifugal machine through a pipeline, the first centrifugal machine is provided with two outlets, one outlet is connected with a waste residue phase storage tank through a pipeline, the other outlet is connected with an inlet of a buffer tank through a pipeline, an outlet of the buffer tank is connected with a feed channel of a second mixer through a pipeline, a second storage tank for storing a second extraction agent is connected with a feed channel of a second mixer through a pipeline, the discharge channel of the second mixer is connected with the second centrifugal machine through a pipeline, the second centrifuge is provided with two outlets, wherein one outlet is connected with the waste residue storage tank through a pipeline, and the other outlet is connected with the finished product storage tank through a pipeline.

As a preferred technical scheme, the first mixer and the second mixer are jet mixers, each jet mixer comprises a mixer body, a receiving chamber, a mixing chamber and a diffusion chamber which are sequentially communicated from left to right are arranged in the mixer body, a discharge channel is arranged on the right side of the diffusion chamber, a first feed channel is connected to the left side of the receiving chamber, the first feed channel is connected with a nozzle, the nozzle is arranged in the receiving chamber, and a second feed channel is connected to the lower side of the receiving chamber.

As a preferable technical scheme, a reinforcing rib is arranged on the outer side of the device body.

According to the preferable technical scheme, the first centrifugal machine and the second centrifugal machine are both centrifugal extraction machines, each centrifugal extraction machine comprises a shell, a rotary drum is mounted in each shell through a rotary drum main bearing, a spiral conveying shaft is mounted in each shell, the spiral conveying shaft and the rotary drum are connected with the same rotary table and driven by the rotary table to move, the rotary table is connected with a transmission mechanism and driven by the transmission mechanism to rotate, an inlet is formed in one side of each shell, a feeding pipe is connected to the inlet to convey materials to the spiral conveying shaft, an adjusting ring is mounted on the inner side wall of each shell, a light phase outlet and a heavy phase outlet are formed in the lower end of each shell, and a slag discharging port is formed in the position, corresponding to the conical end of each rotary.

As the preferred technical scheme, drive mechanism includes motor, V-belt, belt pulley, axis of rotation, the motor passes through V-belt and connects the belt pulley, the belt pulley passes through axis of rotation connection the carousel.

Compared with the prior art, the beneficial effects of the utility model reside in that:

through reasonable design, the extraction liquid can effectively mix and dissolve the cells after the cell crusher crushes the suspended cells in the extraction liquid; in the extraction process, the mechanism is used for extracting twice, before entering a centrifugal extractor each time, the liquid is fully premixed by a mixer and then enters a centrifugal machine again for centrifugal separation to obtain the required liquid, and solid impurities generated in the process can be discharged through a slag discharge port and finally collected by a waste slag phase storage tank. In the whole extraction process, the extraction liquid and the extracting agent are fully mixed, so that the extraction liquid and the extracting agent can be fully dissolved in water to form a multi-phase system, the subsequent centrifugal extraction is facilitated, and the loss of a liquid phase caused by non-dissolution is avoided.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.

Fig. 1 is a schematic structural view of a novel double-aqueous phase extraction mechanism of the present invention;

FIG. 2 is a cross-sectional structural view of a jet mixer of the novel double-water-phase extraction mechanism of the present invention;

fig. 3 is a sectional structure diagram of the centrifugal extractor of the novel double-water-phase extraction mechanism.

In the figure, 1 is a liquid tank, 2 is a cell crusher, 3 is a cooler, 4 is a finished product storage tank, 5 is a first storage tank, 6 is a first mixer, 7 is a first centrifuge, 8 is a waste phase storage tank, 9 is a buffer tank, 10 is a second mixer, 11 is a second storage tank, 12 is a second centrifuge, 14 is a container, 15 is a receiving chamber, 16 is a mixing chamber, 17 is a diffusion chamber, 18 is a discharge passage, 19 is a first feed passage, 20 is a nozzle, 21 is a second feed passage, 22 is a reinforcing rib, 23 is a housing, 24 is a main bearing of a rotary drum, 25 is a rotary drum, 26 is a rotary table, 27 is a spiral conveying shaft, 28 is an inlet, 29 is a feed pipe, 30 is a regulating ring, 31 is a light phase outlet, 32 is a heavy phase outlet, 33 is a waste discharge port, 34 is a V-belt, 35 is a belt pulley, and 36 is a rotary shaft.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that all the directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiments of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.

In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

The invention will now be further described with reference to the accompanying drawings.

Example 1:

referring to fig. 1, the embodiment of the present invention provides a novel dual-aqueous phase extraction mechanism, comprising a liquid tank 1 for storing a liquid to be separated, wherein the liquid tank 1 is connected to a feeding end of a cell crusher 2 through a pipeline, a discharging end of the cell crusher 2 is connected to a cooler 3, the cooler 3 is connected to a feeding channel of a first mixer 6 through a pipeline, a first storage tank 5 for storing a first extractant is connected to a feeding channel of the first mixer 6 through a pipeline, the discharging channel of the first mixer 6 is connected to a first centrifuge 7 through a pipeline, the first centrifuge 7 is provided with two outlets, one of the outlets is connected to a waste residue phase storage tank 8 through a pipeline, the other outlet is connected to an inlet of a buffer tank 9 through a pipeline, the outlet of the buffer tank 9 is connected to a feeding channel of a second mixer 10 through a pipeline, a second storage tank 11 for storing a second extractant is connected to a feeding channel of the second mixer 10 through a pipeline, the discharge channel of the second mixer 10 is connected with a second centrifuge 12 through a pipeline, the second centrifuge 12 is provided with two outlets, one of the outlets is connected with the waste residue phase storage tank 8 through a pipeline, and the other outlet is connected with the finished product storage tank 4 through a pipeline.

In the specific implementation, this embodiment takes a two-aqueous phase extraction system of PEG-inorganic salt as an example, wherein PEG is an abbreviation of polyethylene glycol having the english name of PEG. The first extractant is selected from a mixed solution of PEG and sodium phosphate, the second extractant is NaCl solution, and the liquid to be separated is cell suspension liquid for extracting organic enzyme liquid in the cell suspension liquid. The extraction principle is as follows: after the cell suspension is crushed by the cell crusher 2, the cell suspension is cooled by the cooler 2 and then enters the first mixer 6, the mixed solution of PEG and phosphate also enters the first mixer 6 to be mixed, the mixed solution enters the first centrifuge 7 to be centrifuged, the light phase (PEG + enzyme) is collected to the buffer tank 9 through a pipeline, the first separation is completed, the first separation aims at extracting the organic enzyme to be extracted, other biomolecules (amino acid and the like) in the cell suspension are removed, the other biomolecules are in the heavy phase, and during the actual experiment, the heavy phase outlet of the first centrifuge 7 is connected with a collection box through a pipeline to collect the heavy phase. Then the liquid in the buffer tank 9 enters into the second mixer 10, and simultaneously the phosphate solution also enters into the mixer 10, the concentration of phosphate in the mixed solution is changed, so the distribution balance parameter of the enzyme in the aqueous two-phase system is changed, the enzyme is largely separated out from the PEG and enters into the phosphate solution, the enzyme is collected from the heavy phase outlet of the second centrifuge 12 to the finished product storage tank 4, and the PEG solution from the light phase outlet of the second centrifuge 12 is recycled to the first storage tank 5 through a pipeline.

The embodiment of the utility model provides a holistic extraction organic enzyme, amino acid, biomolecule such as protein mechanism, when implementing, if apply to the laboratory experiment and draw, set up the micropump on the pipeline and be used for providing power for the flow of each liquid. If the device is applied to industrial extraction, a valve is required to be arranged on a pipeline to be matched with a pump so as to control parameters such as flow rate, flow and the like of fluid and meet the requirement of batch automatic extraction.

The utility model discloses preferred embodiment, first blender, second blender are the jet mixer, refer to fig. 2 and show, the jet mixer includes the ware body 14, the ware body 14 is inside to be equipped with from a left side to turn right accepting chamber 15, mixing chamber 16, diffusion chamber 17 that communicate in proper order, discharging channel 18 is seted up on diffusion chamber 17's right side, accept first feedstock channel 19 in the left side of room 15 connection, first feedstock channel 19 connects nozzle 20, nozzle 20 sets up in accepting the room 15, accept the downside of room 15 and connect second feedstock channel 21. And reinforcing ribs 22 are arranged on the outer side of the device body 14.

The mixing principle of the jet mixer in this embodiment is as follows: the two liquids to be mixed enter the receiving chamber 15 from the first feeding channel 19 and the second feeding channel 21 respectively, wherein the first feeding channel 19 is sprayed into the receiving chamber 15 through the nozzle 20, the two liquids enter the mixing chamber 16 to be contacted and mixed, and finally flow out through the discharging channel 18 of the diffusion chamber 17, and the mixing is completed.

The utility model discloses preferred embodiment, first centrifuge 7, second centrifuge 11 are centrifugal extractor, refer to fig. 3 and show, centrifugal extractor includes shell 23, install rotary drum 25 through rotary drum base bearing 24 in the shell 23, install auger delivery axle 27 in the shell 23, auger delivery axle 27 and rotary drum 25 all connect same carousel 26 by carousel 26 drives the motion, carousel 26 connect a drive mechanism by drive mechanism drives rotatoryly, shell 23 one side is provided with entry 28, entry 28 department is connected with conveying pipe 29 and delivers to auger delivery axle 27 department with the material, shell 23 inboard wall mounting adjustable ring 30, shell 23 lower extreme is provided with light phase export 31 and heavy phase export 32, shell 23 is corresponding the cone end department of rotary drum 25 is provided with row cinder notch 33.

The working principle of the centrifugal extractor in this embodiment is as follows: the rotary drum 25 and the spiral conveying shaft 27 are driven by the rotary table to rotate at a high speed at a certain differential rotation speed simultaneously to form a centrifugal force field which is thousands times larger than the gravity field. The separated liquid enters the spiral conveying shaft 27 from the inlet 28, the material transfer and liquid-solid separation between the phases are rapidly completed, solid slag is deposited on the inner skin of the rotary drum 25 and is slowly pushed to the conical end of the rotary drum by the spiral rotor of the spiral conveying shaft 27 to be discharged, the light phase is discharged from the light phase outlet 32 under the action of centrifugal force, and the light phase is discharged from the heavy phase outlet 32 under the action of centrifugal force, so that the centrifugal extraction separation is completed.

Further, drive mechanism includes motor (not shown in the figure), V-belt 34, belt pulley 35, axis of rotation 36, the motor passes through V-belt 34 and connects belt pulley 35, belt pulley 35 passes through axis of rotation 36 connects carousel 26.

The transmission principle is as follows: the motor drives the belt pulley 35 to rotate through the triangle belt 34, the belt pulley 35 drives the turntable 26 to rotate through the rotating shaft 36, and the turntable 26 drives the rotary drum 25 and the spiral conveying shaft 27 to rotate simultaneously. The rotary drum 25 and the screw shaft 27 are not concentric with the rotary table 26, and thus rotate at the same time with a hidden differential rotation speed.

The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not substantially depart from the scope of the embodiments of the present invention, and are intended to be covered by the claims and the specification.

Claims

1. A novel double-aqueous-phase extraction mechanism comprises a liquid tank for storing liquid to be separated, and is characterized in that the liquid tank is connected with a feed end of a cell crusher through a pipeline, a discharge end of the cell crusher is connected with a cooler, the cooler is connected with a feed channel of a first mixer through a pipeline, a first storage tank for storing a first extraction agent is connected with a feed channel of the first mixer through a pipeline, the discharge channel of the first mixer is connected with a first centrifugal machine through a pipeline, the first centrifugal machine is provided with two outlets, one outlet is connected with a waste residue phase storage tank through a pipeline, the other outlet is connected with an inlet of a buffer tank through a pipeline, an outlet of the buffer tank is connected with a feed channel of a second mixer through a pipeline, a second storage tank for storing a second extraction agent is connected with a feed channel of a second mixer through a pipeline, and a discharge channel of the second mixer is connected with a second centrifugal machine through a pipeline, the second centrifuge is provided with two outlets, wherein one outlet is connected with the waste residue storage tank through a pipeline, and the other outlet is connected with the finished product storage tank through a pipeline.

2. The novel double-aqueous-phase extraction mechanism of claim 1, which is characterized in that: first blender, second blender are the jet flow blender, the jet flow blender includes the ware body, the ware is inside to be equipped with from a left side to turn right accepting chamber, mixing chamber, the diffusion chamber that communicates in proper order, discharging channel is seted up on the right side of diffusion chamber, accept first feedstock channel is connected in the left side of room, first feedstock channel connects the nozzle, the nozzle sets up accept indoor, accept the downside of room and connect second feedstock channel.

3. The novel double-aqueous-phase extraction mechanism of claim 2, which is characterized in that: the outer side of the device body is provided with a reinforcing rib.

4. The novel double-aqueous-phase extraction mechanism of claim 1, which is characterized in that: the centrifugal extractor comprises a shell, a rotary drum is installed in the shell through a rotary drum main bearing, a spiral conveying shaft is installed in the shell, the spiral conveying shaft and the rotary drum are connected with a same rotary table, the rotary table is driven to move by the rotary table, the rotary table is connected with a transmission mechanism, the transmission mechanism is driven to rotate, an inlet is formed in one side of the shell, the inlet is connected with a conveying pipe to convey materials to the spiral conveying shaft, an adjusting ring is installed on the inner side wall of the shell, a light phase outlet and a heavy phase outlet are formed in the lower end of the shell, and the shell corresponds to a slag discharging port formed in the conical end of the rotary drum.

5. The novel double-aqueous-phase extraction mechanism of claim 4, which is characterized in that: the transmission mechanism comprises a motor, a V-belt, a belt pulley and a rotating shaft, wherein the motor is connected with the belt pulley through the V-belt, and the belt pulley is connected with the rotating shaft through the rotating shaft.