CN209923331U - Disposable aseptic dyeing culture dish - Google Patents

Abstract

The utility model relates to a disposable aseptic staining culture dish is provided with the equal open-ended tube-shape culture dish body of lower extreme in upper cover and lower cover to be provided with the seal membrane between culture dish body and lower cover. The middle culture dish body can enable the culture medium to form an upper plane and a lower plane. The space formed between the upper plane and the upper cover is used for the growth of microorganisms. After tearing off the PE film, the lower plane was used for dyeing. Thus, the microorganism growth and the dyeing can be effectively separated, and the problem of cross contamination in the dyeing process is avoided.

Description

Disposable aseptic dyeing culture dish

Technical Field

The utility model relates to an aseptic staining culture dish.

Background

The existing screening of microorganisms with extracellular enzyme activity is carried out in a common conventional culture dish, after the microorganisms grow out, corresponding staining solution is added on the surface, and the screening is carried out according to the existence of a transparent ring.

When adopting current culture dish dyeing, because the dip-dyeing of staining fluid, there is cross contamination's the condition between the different bacterial colonies, consequently mix into non-purpose bacterial colony very easily when separation purification, to follow-up work, for example to having the bacterial colony of transparent circle to carry out separation purification and cause the interference.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem that a disposable aseptic staining culture dish is provided, solve and use current culture dish because the dip-dyeing of dyeing liquor causes cross contamination's problem.

The technical scheme of the utility model as follows:

disposable aseptic staining culture dish, including the upper cover and the lower cover of mutually supporting, its characterized in that is provided with the equal open-ended tube-shape culture dish body of lower extreme in upper cover and lower cover inside to be provided with the seal membrane between culture dish body and lower cover.

Preferably, the culture dish further comprises an anti-falling mechanism, wherein the anti-falling mechanism is a tooth-shaped or sheet-shaped mechanism, the root of the tooth-shaped or sheet-shaped mechanism is fixedly connected with the inner wall of the culture dish body, or the tooth-shaped or sheet-shaped mechanism and the culture dish body are integrated.

Further preferably, the sheet-shaped mechanism is a slip-off prevention ring, and the slip-off prevention ring is an annular sheet-shaped mechanism with the root part fixed with the inner wall of the culture dish body or integrally formed.

Still further preferably, the inner edge of the anti-drop ring is provided with a reinforcing sheet, and an included angle is formed between the reinforcing sheet and the anti-drop ring.

Still more preferably, the difference in height between the upper edge of the retaining mechanism and the bottom end surface of the petri dish body 3 is not more than 4 mm.

The utility model has the advantages of:

can effectively avoid the problem of cross contamination caused in the dyeing process. The middle culture dish body can enable the culture medium to form an upper plane and a lower plane. The space formed between the upper plane and the upper cover is used for the growth of microorganisms. After tearing off the PE film, the lower plane was used for dyeing. Thus, the microorganism growth and the dyeing can be effectively separated, and the problem of cross contamination in the dyeing process is avoided.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention.

Fig. 2 is a schematic top view of a culture dish body according to an embodiment of the present invention.

Detailed Description

The invention is further described with reference to the following figures and examples.

Referring to fig. 1, the embodiment of the present invention includes an upper lid 2 and a lower lid 1 having the same radial dimension and fitting with each other, a cylindrical culture dish body 3 having an upper end and a lower end both opened is provided in the upper lid 2 and the lower lid 1, and a sealing film 6 is provided between the culture dish body 3 and the lower lid 1. The sealing film 6 is generally a PE film, and functions to close the culture dish body 3 from the lower end. The outer diameter of the culture dish body 3 is basically consistent with the inner diameter of the upper cover 2 and the lower cover 1 or the former is slightly smaller than the latter.

When in use, the round sealing membrane 6 is firstly paved on the inner bottom surface of the lower cover 1, and the radial dimension of the sealing membrane 6 is required to be larger than the inner diameter dimension of the culture dish body 3. Then, the culture dish body 3 is placed in the lower cover 1, and the lower end of the culture dish body 3 presses the sealing membrane 6 to realize the sealing of the sealing membrane 6 on the lower port of the culture dish body 3. Then, the culture medium and the microbial species are added to the culture dish body 3 from the upper port. Finally, the upper cover 2 is covered.

When the dyeing is needed after a period of culture, the lower cover 1 is opened or the whole part outside the lower cover 1 is taken out from the lower cover 1, the sealing film 6 is torn off, the dyeing liquid is added to the inner bottom surface of the lower cover 1, the lower cover 1 is covered, the dyeing liquid is uniformly dip-dyed after slight movement, the lower cover is kept stand for a plurality of minutes, and redundant dyeing liquid is poured out.

For the culture medium with larger hardness after solidification, the culture medium can be generally attached to the inner wall of the culture dish body 3 after solidification, and the culture medium cannot easily fall off after the sealing film 6 is torn off. However, for a softer culture medium, the anti-dropping mechanism needs to be arranged on the inner wall of the culture dish body 3 to ensure that the culture medium solidified after the sealing film 6 is torn off is still fixed in the culture dish body 3 by the anti-dropping mechanism and does not drop.

For avoiding taking off 3 inner walls landing of culture medium along the culture dish body after seal membrane 6, the embodiment of the utility model further includes root and 3 inner wall looks rigid couplings of culture dish body or the tooth or the piece as an organic whole each other with culture dish body 3.

For example, the retaining ring 4 shown in fig. 1 and 2 serves as a retaining mechanism. The anti-slip ring 4 is in the shape of an annular sheet fixedly connected with the inner wall of the culture dish body 3.

In order to further improve the anti-falling effect of the anti-falling ring 4, the inner edge of the anti-falling ring 4 is provided with a circle of reinforcing sheet 5, and the reinforcing sheet 5 and the anti-falling ring 4 form an included angle of other than 180 degrees, such as an included angle of 30-150 degrees.

Because the liquid level of filling culture medium generally equals or is a little higher than the last edge height of anti-disengaging mechanism to play the effect of the fixed solidified culture medium of anti-disengaging mechanism, consequently the last edge height of anti-disengaging mechanism can not be too high, otherwise can lead to culture medium thickness too big, influences the dyeing effect. Through a plurality of experiments, the height of the upper edge of the anti-falling mechanism can not be higher than the bottom end of the culture dish body 3 by 4mm generally. For example, when the retaining mechanism is designed in the form shown in fig. 1 and 2, i.e., the upper edge of the reinforcing plate 5 is the highest in the retaining mechanism, the height difference between the upper edge of the reinforcing plate 5 and the bottom end surface of the culture dish body 3 is less than 4 mm.

The following is an example of the use of the present invention to perform protease activity staining.

(1) And materials: a sterile casein medium; a disposable sterile staining culture dish (the utility model); trichloroacetic acid staining solution; and (5) a bacterial liquid sample.

(2) Pouring the sterile casein culture medium into a disposable sterile staining culture dish, wherein the culture medium amount does not exceed the upper edge of the reinforcing sheet 5 to form a smooth plane, and cooling and solidifying for later use.

(3) And diluting the bacterial liquid sample by 10 times, 100 times and 1000 times, and coating 100uL of the sample on a casein plate. Cultured at 28 ℃ for 3 days.

(4) And selecting plates (30-70) with proper colony density for staining.

(5) The lower cover 1 of the disposable dyeing culture dish is opened, the PE film (sealing film 6) is torn off, 1mL of trichloroacetic acid dyeing solution is added into the lower cover 1, the whole part outside the lower cover 1 is put back into the lower cover 1, the dyeing solution is uniformly dip-dyed after slight movement, the lower cover is kept stand for several minutes, and redundant dyeing solution is poured off.

(6) And finishing dyeing, and observing the condition of the transparent ring. The upper cover 2 is opened, and the colony with the transparent ring is selected for separation and purification.

Claims (5)

1. A disposable sterile staining culture dish comprises an upper cover (2) and a lower cover (1) which are matched with each other, and is characterized in that a cylindrical culture dish body (3) with an upper end and a lower end which are both open is arranged inside the upper cover (2) and the lower cover (1), and a sealing film (6) is arranged between the culture dish body (3) and the lower cover (1).

2. The disposable aseptic staining dish according to claim 1, further comprising a retaining mechanism, wherein the retaining mechanism is a tooth-shaped or sheet-shaped mechanism having a root portion fixedly connected to the inner wall of the dish body (3) or integrally formed with the dish body (3).

3. The disposable sterile staining dish according to claim 2, wherein the sheet-like means is a slip-off prevention ring (4), and the slip-off prevention ring (4) is a ring-shaped sheet-like means having a root portion fixed to or integrally formed with the inner wall of the dish body (3).

4. A disposable sterile staining dish according to claim 3, wherein the inner edge of the anti-slip ring (4) has a ring of reinforcement tabs (5), the reinforcement tabs (5) forming an angle with the anti-slip ring (4).

5. The disposable sterile staining dish of claim 2, 3 or 4, wherein the height difference between the upper edge of the anti-drop mechanism and the bottom end surface of the dish body (3) is not more than 4 mm.

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