CN209865384U - Semi-automatic pressure device of glass chromatography column - Google Patents

Abstract

The utility model discloses a semi-automatic pressurizing device for a glass chromatographic column, which comprises a nitrogen steel cylinder, wherein the outlet end of the nitrogen steel cylinder is provided with a pressure reducing valve, the pressure reducing valve is communicated with a multi-way valve through a first conduit, a ball valve switch is arranged on the multi-way valve, the multi-way valve is communicated with a flow guide interface through a second conduit, the flow guide interface comprises a flow guide glass tube and a frosted interface, the flow guide interface is communicated with the glass chromatographic column, and an interface clamp is arranged at the joint of the flow guide interface and the glass chromatographic column; the utility model discloses equipment is convenient, air pressure is controllable, can pressurize when purifying the sample to a plurality of glass chromatography columns simultaneously, can be applicable to the glass chromatography column of any specification through the water conservancy diversion interface of changing different pipe diameters, has application scope extensively, easy operation, efficient, good reproducibility etc. and is showing the advantage.

Description

Semi-automatic pressure device of glass chromatography column

Technical Field

The utility model relates to a food safety environmental pollutant detects technical field, especially relates to a semi-automatic pressure device of glass chromatography post.

Background

At present, in the field of detection of environmental pollutants for food safety, for example, detection of dioxin compounds in food samples such as meat, eggs, milk, vegetables and the like, generally comprises the steps of extraction, purification, concentration and the like, wherein a purification link mainly adopts a glass chromatographic column filled with sulfuric acid silica gel, so that a sample solution is subjected to a chemical reaction with concentrated sulfuric acid adsorbed on silica gel when passing through an acidic silica gel chromatographic column, and the purpose of removing fat impurities in the sample solution is achieved. Usually, the sample solution can naturally drop into the sample receiving bottle through the glass chromatographic column by the action of gravity. However, in practical application, for some high-lipid samples, only depending on the action of gravity, the speed of the sample solution passing through the glass chromatographic column is too slow, so that the positions of the reaction between the lipid substances of the samples in the glass chromatographic column and concentrated sulfuric acid are too concentrated, and the generated yellow sticky precipitate often causes the blockage of the glass chromatographic column, so that the purification speed is too slow, the detection efficiency is greatly reduced, and even the sample is completely blocked, thereby causing the failure of the purification of the sample. In order to solve the problem, certain pressure needs to be applied to the glass chromatographic column when the sample is purified by the sulfuric acid silica gel so as to improve the speed of the sample solution passing through the glass chromatographic column and enable the reaction distribution of the sample solution and the concentrated sulfuric acid to be more uniform. Currently, there are two ways to apply pressure to a glass chromatography column: one is a full-automatic special positive pressure device produced by some instrument manufacturers, the price is high, and only a glass chromatographic column with matched specification can be used, so the application range is narrow; in addition, the manual pressurizing mode is adopted, tools such as an ear washing ball or a syringe are generally used for pressurizing by means of hand force of people, the efficiency is low, the pressure is not easy to control, the labor is extremely high, and the operation quantity is very limited.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problems of expensive pressure device or low pressure method efficiency and inconvenient operation in the traditional technology, and providing a semi-automatic pressure device for glass chromatographic columns.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a semi-automatic pressurizing device for a glass chromatographic column comprises a nitrogen steel cylinder, wherein the nitrogen steel cylinder can store nitrogen and provide positive pressure for the system of the utility model; the pressure reducing valve is arranged at the outlet end of the nitrogen steel cylinder and is arranged at the outlet end of the nitrogen steel cylinder, and the pressure of the output nitrogen can be adjusted and controlled; the pressure reducing valve is communicated with the multi-way valve through a first conduit, and the multi-way valve is provided with a ball valve switch which is arranged at the outlet end of the multi-way valve and used for controlling the opening or closing state of nitrogen transmission by the passage; the multi-ported valve passes through second pipe and water conservancy diversion interface intercommunication, the water conservancy diversion interface includes water conservancy diversion glass pipe and dull polish interface, water conservancy diversion interface and glass chromatography column intercommunication, water conservancy diversion interface and glass chromatography column junction are provided with the interface and press from both sides, the interface presss from both sides for fixed water conservancy diversion interface and glass chromatography column junction appear separating when avoiding rising because of the internal gas pressure of system, the junction of water conservancy diversion interface and glass chromatography column.

Preferably, the multi-way valve comprises an inlet end and a plurality of outlet ends, the multi-way valve can divide the transmitted nitrogen into a plurality of paths, and each outlet end can be connected with a flow guide interface through a conduit, so that one nitrogen steel cylinder can pressurize a plurality of glass chromatographic columns.

Preferably, the pressure reducing valve adopts an YQD-6 nitrogen pressure reducing valve, the input pressure is 20MPa, and the adjusting range is 0.1-1.6 MPa.

Preferably, the inlet end of the glass chromatographic column is a frosted interface, and the column can be filled with a purifying filler and used for a purifying reaction container of a sample solution.

Preferably, the inlet end of the diversion glass tube is connected with the second conduit for introducing nitrogen, and the frosted interface is connected with the frosted inlet of the glass chromatographic column for forming a sealing system.

Preferably, the pipe, can freely bend, has certain elasticity for connect relief pressure valve and multi-ported valve to and multi-ported valve and water conservancy diversion interface, so that nitrogen gas is in the utility model discloses transmit in the system.

Preferably, the junction of the pressure reducing valve and the multi-way valve with the first conduit is provided with a sealing clamp, and the junction of the multi-way valve and the flow guide interface with the second conduit is provided with a sealing clamp.

Compared with the prior art, the utility model discloses possess following advantage:

the utility model discloses equipment is convenient, air pressure is controllable, can pressurize when purifying the sample to a plurality of glass chromatography columns simultaneously, can be applicable to the glass chromatography column of any specification through the water conservancy diversion interface of changing different pipe diameters, has application scope extensively, easy operation, efficient, good reproducibility etc. and is showing the advantage.

Drawings

Fig. 1 is a schematic structural view of a semi-automatic pressurizing device for a glass chromatographic column provided by the present invention;

in the figure: the device comprises a nitrogen gas steel cylinder 1, a pressure reducing valve 2, a first conduit 3, a multi-way valve 4, a ball valve switch 5, a conduit 6, a flow guide interface 7, a flow guide glass tube 7-1, a ground glass interface 7-2, an interface clamp 8, a glass chromatographic column 9 and a sealing clamp 10.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1, a semi-automatic pressure device of glass chromatography column, including nitrogen gas steel bottle 1, 1 exit end of nitrogen gas steel bottle is provided with relief pressure valve 2, relief pressure valve 2 communicates with multi-way valve 4 through first pipe 3, is provided with ball valve switch 5 on the multi-way valve 4, and multi-way valve 4 communicates with water conservancy diversion interface 7 through second pipe 6, and water conservancy diversion interface 7 includes water conservancy diversion glass pipe 7-1 and dull polish interface 7-2, and water conservancy diversion interface 7 communicates with glass chromatography column 9, and water conservancy diversion interface 7 and glass chromatography column 9 junction are provided with interface clamp 8.

The multi-way valve 4 comprises an inlet end and a plurality of outlet ends, and each outlet end can be connected with a flow guide connector 7 through a conduit; the pressure reducing valve is YQD-6 nitrogen pressure reducing valve, the input pressure is 15Mpa, and the adjusting range is 0.1-1.6 Mpa; the inlet end of the glass chromatographic column 9 is a frosted interface, and the column can be filled with purification filler and used for a purification reaction container of a sample solution; the inlet end of a diversion glass tube 7-1 is connected with a second conduit 6 for introducing nitrogen, and a frosted interface 7-2 is connected with a frosted inlet of a glass chromatographic column 9 for forming a sealing system; the joints of the pressure reducing valve 2, the multi-way valve 4 and the first conduit 3 are provided with sealing clamps 10, and the joints of the multi-way valve 4, the flow guide interface 7 and the second conduit 6 are provided with sealing clamps 10.

Now, the operation principle of the present invention is described as follows:

as shown in fig. 1, which is a schematic structural diagram of a semi-automatic pressurizing device for a glass chromatography column of the present invention, the device comprises a 40 l nitrogen gas steel cylinder 1, wherein the nitrogen gas steel cylinder 1 stores high-purity nitrogen gas and has a charging pressure of 20 Mpa; the outlet end of the nitrogen steel cylinder 1 is subjected to pressure output control by a pressure reducing valve 2, and the pressure reducing valve 2 is in a closed state in an initial state; the pressure reducing valve 2 is connected with the inlet end of a six-way valve 4 through a first conduit 3 made of rubber, each outlet end of the six-way valve 4 is subjected to flow rate control by a ball valve switch 5, the initial state of the ball valve switch is an opening state, and six outlet ends of the six-way valve 4 are respectively connected with diversion glass tubes 7-1 of six diversion interfaces 7 through six second conduits 6 made of rubber; connecting a No. 24 standard-mouth ground glass interface 7-2 of the flow guide interface 7 with a ground inlet of a glass chromatographic column 9 provided with a No. 24 standard mouth, and fixing the ground glass interface 7-2 and the glass chromatographic column 9 by using a stainless steel interface clamp 8; slightly opening the pressure reducing valve 2, observing and determining the output pressure of the pressure reducing valve 2 according to the liquid dropping speed at the outlet end of the chromatographic column, and further finely adjusting the pressure of each pressurizing flow path or closing the flow path by adjusting a ball valve switch 5 to stop pressurizing; after all the liquid in the glass chromatographic column flows out, the pressure reducing valve 2 is closed, the stainless steel interface clamp 8 is taken down, the flow guide interface 7 is taken out, and the whole glass chromatographic column pressurizing process is completed.

In the present invention, unless otherwise expressly stated or limited, the terms "sliding", "rotating", "fixed", "provided", and the like are to be understood in a broad sense, e.g. as welded connections, as bolted connections, or as integral parts; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims (6)

1. The utility model provides a semi-automatic pressure device of glass chromatography post, includes nitrogen gas steel bottle (1), its characterized in that, nitrogen gas steel bottle (1) exit end is provided with relief pressure valve (2), relief pressure valve (2) communicate through first pipe (3) and multi-pass valve (4), be provided with ball valve switch (5) on multi-pass valve (4), multi-pass valve (4) communicate with water conservancy diversion interface (7) through second pipe (6), water conservancy diversion interface (7) are including water conservancy diversion glass pipe (7-1) and dull polish interface (7-2), water conservancy diversion interface (7) and glass chromatography post (9) intercommunication, water conservancy diversion interface (7) and glass chromatography post (9) junction are provided with interface clamp (8).

2. A semi-automatic pressurizing device for glass chromatography columns according to claim 1, characterized in that the multi-way valve (4) comprises an inlet end and a plurality of outlet ends, and each outlet end is connected with a flow guide interface (7) through a conduit.

3. The semi-automatic pressurizing device for glass chromatographic column as claimed in claim 1, wherein the pressure reducing valve is YQD-6 nitrogen pressure reducing valve, the input pressure is 20Mpa, and the regulating range is 0.1-1.6 Mpa.

4. The semi-automatic pressurizing device for the glass chromatographic column as claimed in claim 1, wherein the inlet end of the glass chromatographic column (9) is a frosted interface, and the column can be filled with purifying filler and used for a purifying reaction container of a sample solution.

5. The semi-automatic pressurizing device for glass chromatographic columns according to claim 1, characterized in that the inlet end of the flow guide glass tube (7-1) is connected with the second conduit (6) for introducing nitrogen, and the frosted interface (7-2) is connected with the frosted inlet of the glass chromatographic column (9) for forming a sealing system.

6. The semi-automatic pressurizing device of the glass chromatographic column as claimed in claim 1, wherein a sealing clamp (10) is arranged at the joint of the pressure reducing valve (2) and the multi-way valve (4) with the first conduit (3), and a sealing clamp (10) is arranged at the joint of the multi-way valve (4) and the flow guide interface (7) with the second conduit (6).