CN209810441U

CN209810441U - Vascular endothelial cell culture apparatus

Info

Publication number: CN209810441U
Application number: CN201920507434.9U
Authority: CN
Inventors: 陈鹏; 沈志强; 李璠; 杨仁华; 何波
Original assignee: Kunming Medical University
Current assignee: Kunming Medical University
Priority date: 2019-04-15
Filing date: 2019-04-15
Publication date: 2019-12-20
Anticipated expiration: 2029-04-15

Abstract

The utility model discloses a vascular endothelial cell culture apparatus, the power distribution box comprises a box body, be provided with the commentaries on classics board in the inner chamber of box, the first solid fixed ring of top fixedly connected with between two first supports, the inside cover of first solid fixed ring is equipped with the sleeve, the top and the bottom of sleeve outer wall are all fixed the spacing ring that has cup jointed, the top and the bottom of first solid fixed ring inner wall all set up with spacing ring matched with spacing groove, it has first magnetite ring just to be located telescopic outer wall fixed cup joints between two spacing rings, first solid fixed ring inner wall has cup jointed the second magnetite ring with the corresponding department in first magnetite ring position is fixed, telescopic inside interlude is connected with the test tube. The utility model discloses utilize first magnet ring and second magnet ring matched with mode that sets up, cushion the sleeve when centrifuging through mutual repulsion force between first magnet ring and the second magnet ring, protect the test tube when centrifuging, improve the success rate of experiment.

Description

Vascular endothelial cell culture apparatus

Technical Field

The utility model relates to a cell culture field, in particular to vascular endothelial cell culture apparatus.

Background

One of the characteristics of cell culture needs to be eliminated because it needs to be proliferated on a large scale. The objective of digestion makes its adhesion albumen decompose, makes its dispersion, and be favorable to the cell to carry out nutrition exchange, accomplishes the activity to the life, in the process of in vitro culture, because do not construct the circulation system of completion in vitro, nutrient composition, metabolic waste can't separate clearly, so cell adhesion leads to cell nutrition to supply not good, and because nutrient composition and metabolic waste all mix together, so need regularly to change liquid, because collect the cell through the centrifugal mode, accomplish the completion to its screening and change liquid, make its abundant growth.

Traditional test tube is generally made by glass, and cell culture device is when carrying out centrifugal motion, and centrifugal speed is too big, and test tube wall and test tube fixed position department do not have the component that can supply the buffering to it is just bigger with test tube fixed position department relative power to lead to the test tube, and relative power is big after certain degree, extrudees the test tube wall, causes not your destruction to the test tube wall.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a vascular endothelial cell culture device to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a vascular endothelial cell culture device, which comprises a box body, wherein a rotating plate is arranged in the inner cavity of the box body, the two sides of the upper surface of the rotating plate are fixedly connected with first brackets, the top between the two first brackets is fixedly connected with a first fixing ring, the first fixing ring is internally sleeved with a sleeve, the top and the bottom of the outer wall of the sleeve are fixedly sleeved with limit rings, the top and the bottom of the inner wall of the first fixing ring are both provided with limit grooves matched with the limit rings, a first magnet ring is fixedly sleeved between the two limit rings and positioned on the outer wall of the sleeve, a second magnet ring is fixedly sleeved at the position of the inner wall of the first fixing ring corresponding to the position of the first magnet ring, and the like magnetic poles of the first magnet ring and the second magnet ring are opposite, the inner wall of the sleeve is fixedly sleeved with a rubber pad, and the inside of the sleeve is connected with a test tube in an inserting manner.

Preferably, the below of test tube and the last fixed surface who is located the commentaries on classics board are connected with the connecting rod, the top fixedly connected with base of connecting rod, the arc recess has been seted up at the top of base, nine rubber strips of equidistance fixedly connected with on the inner wall of arc recess at least.

Preferably, the outside of changeing the board is provided with the solid fixed ring of second, the solid fixed ring's of second inner wall seted up with changeing board matched with ring channel, the outer wall of changeing the board and be located the inner chamber of ring channel and be provided with the ball.

Preferably, the top and the bottom of the inner wall of the annular groove are both provided with a clamping groove matched with the ball, one side of the ball is in rolling connection with the rotating plate, and the other side of the ball is in rolling connection with the inner wall of the annular groove.

Preferably, there is a motor in the bottom of the inner cavity of the box body through a second bracket, the output end of the motor is connected with a rotating rod in a transmission manner, the top end of the rotating rod is fixedly connected with a connecting platform, and the top end of the connecting platform is fixedly connected with the lower surface of the rotating plate.

Preferably, the top of the box body is movably clamped with a top plate, and the upper surface of the top plate is fixedly connected with a handle.

Preferably, the motor is electrically connected with an external power supply through an external motor switch.

The utility model discloses a technological effect and advantage:

1. the utility model discloses utilize first magnet ring and second magnet ring matched with setting mode, cushion the sleeve when centrifuging through mutual repulsion force between first magnet ring and the second magnet ring, prevent that the sleeve in the centrifugation from striking the inner wall of first solid fixed ring to cushion the test tube when centrifuging, protect the test tube when centrifuging, and then increased the life of test tube, avoid the destruction of test tube to cause the destruction to whole cell culture experiment, improve the success rate of experiment;

2. the utility model utilizes the setting mode of matching the motor and the rotating plate, and drives the connecting platform to rotate through the motor, thereby driving the rotating plate to rotate, and further realizing the high-speed centrifugal treatment of the cells in the inner cavity of the test tube;

3. the utility model discloses utilize the solid fixed ring of second and ball matched with to set up the mode, go on spacingly to the upper and lower position when changeing the board rotation through the second fixed ring, when preventing to connect the platform and be connected not fastening with the bull stick, can break away from each other between connection platform and the bull stick, the board that changes this moment receives centrifugal force can the departure its original position, influences going on of cell culture experiment.

Drawings

Fig. 1 is a schematic front structural view of the present invention.

Fig. 2 is a schematic view of the internal structure of the sleeve of the present invention.

Fig. 3 is an enlarged schematic view of a portion a in fig. 1 according to the present invention.

In the figure: 1. a box body; 2. rotating the plate; 3. a first bracket; 4. a first retaining ring; 5. a sleeve; 6. a limiting ring; 7. a limiting groove; 8. a first magnet ring; 9. a second magnet ring; 10. a rubber pad; 11. a test tube; 12. a base; 13. a rubber strip; 14. a second retaining ring; 15. a ball bearing; 16. a motor; 17. a connecting table; 18. a top plate; 19. a handle.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model provides a vascular endothelial cell culture device as shown in figures 1-3, which comprises a box body 1, a top plate 18 is movably clamped at the top of the box body 1, a handle 19 is fixedly connected with the upper surface of the top plate 18, the top plate 18 is opened, the test tube 11 can be conveniently placed, the rotating plate 2 is arranged in the inner cavity of the box body 1, the bottom end of the inner cavity of the box body 1 is fixedly provided with a motor 16 through a second bracket, the output end of the motor 16 is connected with a rotating rod in a transmission way, the top end of the rotating rod is fixedly connected with a connecting platform 17, the top end of the connecting platform 17 is fixedly connected with the lower surface of the rotating plate 2, the motor 16 drives the connecting table 17 to rotate, thereby driving the rotating plate 2 to rotate, and then realize carrying out high-speed centrifugal treatment to the cell in the 11 inner chambers of test tube, change the equal fixedly connected with first support 3 in both sides of 2 upper surfaces of board, the first solid fixed ring 4 of top fixedly connected with between two first supports 3.

The inside cover of first solid fixed ring 4 is equipped with sleeve 5, the top and the bottom of sleeve 5 outer wall are all fixed the cover and have been connect spacing ring 6, the top and the bottom of first solid fixed ring 4 inner wall all set up with spacing ring 6 matched with spacing groove 7, the outer wall that just is located sleeve 5 between two spacing rings 6 is fixed the cover and has been connect first magnet ring 8, first solid fixed ring 4 inner wall is fixed with the corresponding department in first magnet ring 8 position and has been cup jointed second magnet ring 9, and first magnet ring 8 is relative with the same polarity magnetic pole of second magnet ring 9, the inner wall of sleeve 5 is fixed and has been cup jointed rubber pad 10, anti-skidding texture has been seted up on the rubber pad 10, the grabbing power when increasing and contacting with test tube 11, the inside interlude of sleeve 5 is connected with test tube 11.

Test tube 11's below and be located the last fixed surface of commentaries on classics board 2 and be connected with the connecting rod, the top fixedly connected with base 12 of connecting rod, the arc recess has been seted up at the top of base 12, nine rubber strips 13 at least of equidistance fixedly connected with on the inner wall of arc recess, and a plurality of's rubber strip 13 both can have the holding power to test tube 11, will have the cushioning effect between test tube 11 and the base 12.

The outside of commentaries on classics board 2 is provided with the solid fixed ring of second 14, the solid fixed ring of second 14's inner wall has been seted up and has been changeed board 2 matched with ring channel, the outer wall of commentaries on classics board 2 just is provided with ball 15 in being located the inner chamber of ring channel, ring channel inner wall's top and bottom all seted up with ball 15 matched with draw-in groove, ball 15 one side and commentaries on classics board 2 roll connection, ball 15's opposite side and the inner wall roll connection of ring channel, it is spacing to changeing the upper and lower position when board 2 is rotatory through the solid fixed ring of second 14, when preventing to connect platform 17 and bull stick and being connected not fastening, can break away from each other between connection platform 17 and the bull stick, commentaries on classics board 2.

The motor 16 is electrically connected to an external power source through an external motor switch.

This practical theory of operation:

when the test tube device is used, the test tube 11 is inserted into the inner cavity of the sleeve 5 from top to bottom until the bottom end of the test tube 11 is contacted with the rubber strips 13 on the base 12, at the moment, a plurality of rubber strips 13 not only can support the test tube 11, but also can buffer the test tube 11 and the base 12, after the test tube 11 is placed in the position, a motor switch is turned on, the connecting table 17 is driven by the motor 16 to rotate, so that the rotating plate 2 is driven to rotate, and then high-speed centrifugal treatment is carried out on cells in the inner cavity of the test tube 11, in the rotating process of the test tube 11, the sleeve 5 can move towards one side of the first fixing ring 4 under the action of centrifugal force in the first fixing ring 4, at the moment, the limiting ring 6 slightly slides towards one side of the limiting groove 7, a movement buffer space is provided for the sleeve 5, and the sleeve 5 in the centrifugal process is buffered through the mutual repulsive force between the first magnet ring 8 and the, prevent that sleeve 5 among the centrifugal process from striking the inner wall of first solid fixed ring 4 to test tube 11 when centrifuging cushions, and then protects test tube 11 when centrifuging.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A vascular endothelial cell culture apparatus comprises a box body (1), and is characterized in that: the magnetic field generator is characterized in that a rotating plate (2) is arranged in an inner cavity of the box body (1), first supports (3) are fixedly connected to two sides of the upper surface of the rotating plate (2), a first fixing ring (4) is fixedly connected to the top between the two first supports (3), a sleeve (5) is sleeved inside the first fixing ring (4), limiting rings (6) are fixedly sleeved on the top and the bottom of the outer wall of the sleeve (5), limiting grooves (7) matched with the limiting rings (6) are formed in the top and the bottom of the inner wall of the first fixing ring (4), a first magnet ring (8) is fixedly sleeved on the outer wall between the two limiting rings (6) and located on the sleeve (5), a second magnet ring (9) is fixedly sleeved on the position corresponding to the position of the first magnet ring (8) on the inner wall of the first fixing ring (4), and like magnetic poles of the first magnet ring (8) and the second magnet ring (9) are opposite, the rubber pad (10) is fixedly sleeved on the inner wall of the sleeve (5), and test tubes (11) are inserted and connected into the sleeve (5).

2. The vascular endothelial cell culture device according to claim 1, wherein: the below of test tube (11) and the last fixed surface who is located commentaries on classics board (2) are connected with the connecting rod, top fixedly connected with base (12) of connecting rod, the arc recess has been seted up at the top of base (12), nine rubber strips (13) of equidistance fixedly connected with on the inner wall of arc recess at least.

3. The vascular endothelial cell culture device according to claim 1, wherein: the outside of commentaries on classics board (2) is provided with the solid fixed ring of second (14), the inner wall of the solid fixed ring of second (14) seted up with commentaries on classics board (2) matched with ring channel, the outer wall of commentaries on classics board (2) just is provided with ball (15) in the inner chamber that is located the ring channel.

4. The vascular endothelial cell culture device according to claim 3, wherein: the top and the bottom of ring channel inner wall all seted up with ball (15) matched with draw-in groove, one side and commentaries on classics board (2) roll connection of ball (15), the opposite side and the inner wall roll connection of ring channel of ball (15).

5. The vascular endothelial cell culture device according to claim 1, wherein: the bottom of box (1) inner chamber has motor (16) through second support fixed mounting, the output transmission of motor (16) is connected with the bull stick, the top fixedly connected with of bull stick connects platform (17), just the top of connecting platform (17) is connected with the lower fixed surface of commentaries on classics board (2).

6. The vascular endothelial cell culture device according to claim 1, wherein: the top of the box body (1) is movably clamped with a top plate (18), and the upper surface of the top plate (18) is fixedly connected with a handle (19).

7. The vascular endothelial cell culture device according to claim 5, wherein: the motor (16) is electrically connected with an external power supply through an external motor switch.