CN209808619U - Detection system for experimental mouse self-lighting addiction behavior - Google Patents
Detection system for experimental mouse self-lighting addiction behavior Download PDFInfo
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- CN209808619U CN209808619U CN201821271909.0U CN201821271909U CN209808619U CN 209808619 U CN209808619 U CN 209808619U CN 201821271909 U CN201821271909 U CN 201821271909U CN 209808619 U CN209808619 U CN 209808619U
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Abstract
The utility model belongs to the field of animal ethology detection, and relates to a detection system for the autonomous light-feeding addiction behavior of an experimental mouse, which mainly comprises an experimental box, an effective contact hole, an ineffective contact hole, a foot sole electric shock rod, a tray, a control box, a bidirectional connecting wire, a camera, an optical fiber jumper, a laser, an input/output controller and a computer; the side wall of the experimental box is provided with an effective contact hole and an ineffective contact hole, the bottom of the experimental box is provided with a tray, and the tray is provided with a foot sole electric shock rod; the control box is arranged outside the side wall of the experimental box and is connected with the input/output controller through a bidirectional connecting wire; one port of the input/output controller is connected with the computer, and the other end of the input/output controller is connected with the laser; the laser is connected with an optical fiber jumper. The result of use shows, through the system can not only directly appraise the reward loop in the brain, carry out relevant loop research, can also simulate human addiction action very well, carry out addiction action characterization analysis.
Description
Technical Field
The utility model belongs to animal behaviours detects the field, relates to an experimental mice independently gives the detecting system of light addiction action, and this system is applicable to the analysis of animal addiction action sign and the research of reward relevant neural circuit level.
Background
In the prior art, the model of drug addiction behavior closest to humans is self-administered in experimental animal models of drug addiction; the method comprises the steps that a jugular vein of an experimental animal is placed in advance, when the experimental animal treads a specific device (such as a small plate or a nose touches a specific small hole) each time, medicines such as cocaine or heroin can be actively obtained through veins, and a technician observes the addiction behavior of the animal by recording the effective pedal and the effective nose touch quantity of the experimental animal. At present, the related experimental technical method of the prior art is mainly suitable for experimental rats, and the operation of the experimental mouse self-administration system depends on the survival rate of jugular vein catheterization of the experimental mouse, and research practices show that the success rate of the operation is low, the postoperative experimental mouse is slow to recover, the survival time is short, the smoothness of the postoperative venous catheterization is difficult to maintain, and the peripheral blood vessels of the postoperative jugular vein catheterization are easy to proliferate and block, so that the experimental mouse self-administration model cannot be effectively used for researching the addiction behaviors of experimental animals. However, the preparation of the tool mouse is far more than that of the tool rat at present, which limits the use of the tool mouse for a self-administration model to a certain extent, thereby limiting the deep research of a reward nerve loop to a certain extent.
Based on the current state of the art, the designer of the application intends to provide a system for detecting the behavior of the voluntary light addiction of the experimental mouse, and the system is suitable for the analysis of the animal addiction behavior characterization and the research of the reward-related neural circuit level.
Disclosure of Invention
The utility model aims at overcoming the defects of the prior art and providing a self-sufficient light addiction behavior detection system for experimental mice, which is suitable for the analysis of the addiction behavior representation of experimental animals and the research of the reward related neural loop level; furthermore, not only can direct activation of the reward nerve loop be achieved using this system, but other peripheral side effects of addictive drugs can also be reduced.
The utility model discloses experimental mouse self-sufficient light addiction behavior detection system, its characterized in that mainly comprises experimental box 1, effective contact hole 4, ineffective contact hole 5, sole electric shock pole 6, tray 7, control box 8, two-way connecting wire 9, camera 11, optic fibre wire jumper 13, laser instrument 14, input/output controller 15 and computer 16;
the side wall of the experimental box 1 is provided with an effective contact hole 4 and an ineffective contact hole 5, the bottom of the experimental box 1 is provided with a tray 7, and the tray 7 is provided with a sole electric shock rod 6; a control box 8 is arranged outside the side wall of the experimental box 1, and the control box 8 is connected with an input/output controller 15 through a bidirectional connecting wire 9; one port of the input/output controller 15 is connected with the computer 16, and the other end of the input/output controller 15 is connected with the laser 14; the laser 14 is connected with an optical fiber jumper 13;
in the utility model, a loudspeaker 2 and a signal lamp 3 are arranged on the side wall of one side of the experimental box 1, and a room lamp 10 is arranged on the other side wall of the experimental box 1;
the top end of the experimental box 1 is provided with a universal wheel 12, and the optical fiber jumper 13 is fixedly connected with the laser 14 through the universal wheel 12;
in one embodiment of the present invention, a probe is embedded in the brain region of the experimental mouse, the probe is connected to a two-in-one optical fiber 13, and the optical fiber is connected to the laser 14 through the universal wheel 12; wherein, the right hole is an effective contact hole 4, and the nose of the mouse can trigger laser, sound and signal light for one time; the left side hole is an invalid contact hole 5, and the nose of the mouse can not trigger laser, sound and signal lamps.
When in use, the utility model is used,
(1) the expression of photosensitive protein in the brain of the mouse and the embedding of a probe are carried out through the craniocerebral surgical operation of the mouse, the operation is simple, and the postoperative survival rate of the mouse is close to 100 percent; connecting the optical fiber with a probe embedded in the head of the mouse; an infrared detector is arranged in a small hole of the behavior detection box, when a mouse nose touches an effective small hole, infrared light is cut off, and then primary laser is triggered to activate a reward loop in the brain; when the nose of the mouse touches another ineffective pinhole, the laser cannot be triggered; thus, mice develop a preference for nasal palpable effective ostia; with the increase of the number of nasal palpation, reward the loop in the brain is strengthened, the mouse forms the addict of the effective aperture of nasal palpation finally;
(2) after the mouse is addicted to the self-sufficient light behavior, the nose touches the effective small hole to give laser and simultaneously can trigger foot bottom electric shock; after the normal mouse experiences plantar electric shock, the normal mouse is aversive to the nose touching the pinhole and the nose touching times are obviously reduced; after the negative event of the sole electric shock is associated with the reward giving event, the addict mouse still keeps a high number of nasal triggers, and the behavior can well simulate the behavior characteristics of addicts for obtaining medicines without any cost; the vola electric energy equipped by the behavior detection box simultaneously detects the acquisition, consolidation and extraction of mouse conditional fear memory, and carries out memory-related research work; in addition, if the ostia on one side of the nasal palpation is associated with a negative event such as sole electric shock, and the ostia on the other side of the nasal palpation is associated with a positive reward event such as activation of a specific reward loop, a go no-go related experiment can be performed, and relevant researches such as reward/aversion behaviors and motivations of the mouse can be discussed;
(3) a frequency-adjustable loudspeaker and a lamp are arranged in the self-sufficient light behavior detection box, and when the nose touches the small hole, sound and light with certain frequency can be triggered to serve as signals of self-sufficient light or sole electric shock; when a signal appears, the addicted mice can have spontaneous nasal palpation behavior, and the mice receiving the foot-bottom electric shock can have catalepsy behavior; the acousto-optic signal can be used as a clue to induce the nose of the mouse to touch and obtain the light seeking behavior of the laser; the acousto-optic signal induces the nose to touch and can carry out regression and reconstruction of addictive behaviors without giving light; the acousto-optic signals can also be used as clues to extract reward memory and conditional fear memory, and carry out related researches such as memory and the like.
The use result shows that the system of the utility model needs to combine with the optogenetic equipment and the virus infection system, a light sensitive channel which can be excited by a certain wavelength is expressed in a certain neuron or nerve loop of the mouse, and the mouse can trigger the laser to emit laser with a specific wavelength when touching the small hole by the nose, so as to activate the observed neuron or nerve loop; if the number of active nasal touches of the mouse in a specified time is significantly more than that of the ineffective pinholes without laser triggering, the addict behavior that the mouse touches the specific pinholes through the nose is generated; if the mouse still keeps a high number of nasal palpation behaviors after the mouse is addicted to the light by touching the nose and simultaneously triggers foot-sole electric shock, the mouse further generates compulsive characteristics for the autonomous nasal palpation behaviors, and the clinical characteristics of the drug are obtained by highly simulating addicts without counting any consequence; the system can directly identify reward loops in the brain to carry out related loop research, and can also simulate human addiction behaviors very well to carry out analysis of characterization of the addiction behaviors.
The parts and components required for the preparation of the detection system and the experimental equipment required for the use are all known by the technical personnel in the field or can be obtained through commercial channels.
For the convenience of understanding, the self-sufficient light addiction behavior detection system for experimental mice of the present invention will be described in detail below with reference to the specific drawings and examples. It is to be expressly understood that the specific examples and drawings are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application.
Drawings
FIG. 1 is a schematic diagram of the self-sufficient light addiction behavior detection system of the experimental mouse of the present invention,
wherein, 1 is the experimental box, 2 is the speaker, 3 is the signal lamp, 4 is effective contact hole, 5 is invalid contact hole, 6 is sole shock rod, 7 is the tray, 8 is the control box, 9 is the two-way connecting wire, 10 is the room lamp, 11 is the camera, 12 is the universal wheel, 13 is the optic fibre jumper wire, 14 is the laser instrument, 15 input/output controllers, 16 is the computer.
Detailed Description
Example 1
As shown in fig. 1, the system for detecting self-sufficient light addiction behavior of experimental mice mainly comprises an experimental box 1, an effective contact hole 4, an ineffective contact hole 5, a sole electric shock rod 6, a tray 7, a control box 8, a bidirectional connecting line 9, a camera 11, an optical fiber jumper 13, a laser 14, an input/output controller 15 and a computer 16;
the side wall of the experimental box 1 is provided with an effective contact hole 4 and an ineffective contact hole 5, the bottom of the experimental box 1 is provided with a tray 7, and the tray 7 is provided with a sole electric shock rod 6; a control box 8 is arranged outside the side wall of the experimental box 1, and the control box 8 is connected with an input/output controller 15 through a bidirectional connecting wire 9; one port of the input/output controller 15 is connected with the computer 16, and the other end of the input/output controller 15 is connected with the laser 14; the laser 14 is connected with an optical fiber jumper 13;
a loudspeaker 2 and a signal lamp 3 are also arranged on the side wall of one side of the experimental box 1, and a room lamp 10 is arranged on the other side wall of the experimental box 1;
the top end of the experimental box 1 is provided with a universal wheel 12, and the optical fiber jumper 13 is fixedly connected with the laser 14 through the universal wheel 12.
When in use, the utility model is used,
(1) the expression of photosensitive protein in the brain of the mouse and the embedding of a probe are carried out through the craniocerebral surgical operation of the mouse, the operation is simple, and the postoperative survival rate of the mouse is close to 100 percent; connecting the optical fiber with a probe embedded in the head of the mouse; an infrared detector is arranged in a small hole of the behavior detection box, when a mouse nose touches an effective small hole, infrared light is cut off, and then primary laser is triggered to activate a reward loop in the brain; when the nose of the mouse touches another ineffective pinhole, the laser cannot be triggered; thus, mice develop a preference for nasal palpable effective ostia; with the increase of the number of nasal palpation, reward the loop in the brain is strengthened, the mouse forms the addict of the effective aperture of nasal palpation finally;
(2) after the mouse is addicted to the self-sufficient light behavior, the nose touches the effective small hole to give laser and simultaneously can trigger foot bottom electric shock; after the normal mouse experiences plantar electric shock, the normal mouse is aversive to the nose touching the pinhole and the nose touching times are obviously reduced; after the negative event of the sole electric shock is associated with the reward giving event, the addict mouse still keeps a high number of nasal triggers, and the behavior can well simulate the behavior characteristics of addicts for obtaining medicines without any cost; the vola electric energy equipped by the behavior detection box simultaneously detects the acquisition, consolidation and extraction of mouse conditional fear memory, and carries out memory-related research work; in addition, if the ostia on one side of the nasal palpation is associated with a negative event such as sole electric shock, and the ostia on the other side of the nasal palpation is associated with a positive reward event such as activation of a specific reward loop, a go no-go related experiment can be performed, and relevant researches such as reward/aversion behaviors and motivations of the mouse can be discussed;
(3) a frequency-adjustable loudspeaker and a lamp are arranged in the self-sufficient light behavior detection box, and when the nose touches the small hole, sound and light with certain frequency can be triggered to serve as signals of self-sufficient light or sole electric shock; when a signal appears, the addicted mice can have spontaneous nasal palpation behavior, and the mice receiving the foot-bottom electric shock can have catalepsy behavior; the acousto-optic signal can be used as a clue to induce the nose of the mouse to touch and obtain the light seeking behavior of the laser; the acousto-optic signal induces the nose to touch and can carry out regression and reconstruction of addictive behaviors without giving light; the acousto-optic signals can also be used as clues to extract reward memory and conditional fear memory, and carry out related researches such as memory and the like.
Example 2 selflight experiment of nerve projection from nucleus accumbens to ventral tegmental area
Expressing an activated light sensitive protein ChR2 in mouse nucleus accumbens neurons through a virus microinjection operation, and implanting a probe in a mouse ventral tegmental area; after two weeks of surgical recovery, the embedded probe at the mouse head was connected to the fiber and the mouse was placed in a self-contained light behavior detection box for free exploration. When the mouse touches the effective aperture, the mouse is given sound stimulation (1 second, 85 decibels, 2000 Hz) and is given laser (20 Hz, 20 milliwatt) once, and the refractory period is 1 second; training time is 1 hour per day; the mice stably form effective ostiole nasal exposure times of 800 times/hour and ineffective ostiole nasal exposure times of 20 times/hour; the results indicate that the mice are addicted to autarkic light projected by nerves activating the nucleus accumbens to the ventral tegmental area; showing the mouse undergoing nose strike, the yellow signal light shines and the overhead fiber transmits 470nm blue laser light.
Experimental result shows, through detecting system can not only directly appraise experiment mouse intracerebral reward loop, carry out relevant loop research, the addiction action that can also be very good anthropomorphic dummy carries out addiction action characterization analysis.
Claims (3)
1. The system for detecting the autonomous light addiction behavior of the experimental mouse is characterized by mainly comprising an experimental box (1), an effective contact hole (4), an ineffective contact hole (5), a sole electric shock rod (6), a tray (7), a control box (8), a bidirectional connecting wire (9), a camera (11), an optical fiber jumper (13), a laser (14), an input/output controller (15) and a computer (16);
an effective contact hole (4) and an ineffective contact hole (5) are arranged on the side wall of the experiment box (1), a tray (7) is arranged at the bottom of the experiment box (1), and a sole electric shock rod (6) is arranged on the tray (7); a control box (8) is arranged outside the side wall of the experimental box (1), and the control box (8) is connected with an input/output controller (15) through a bidirectional connecting line (9); one port of the input/output controller (15) is connected with the computer (16), and the other end of the input/output controller (15) is connected with the laser (14); the laser (14) is connected with an optical fiber jumper (13);
the top of experimental box (1) is equipped with universal wheel (12), optic fibre wire jumper (13) pass through universal wheel (12) with laser instrument (14) fixed connection.
2. The system for detecting the autonomic light addiction behavior of the experimental mice as claimed in claim 1, wherein a loudspeaker (2) and a signal lamp (3) are further arranged on one side wall of the experimental box (1).
3. The system for detecting the addiction behavior of laboratory mice as claimed in claim 1, wherein a room light (10) is provided on the other side wall of the laboratory box (1).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201821271909.0U CN209808619U (en) | 2018-08-08 | 2018-08-08 | Detection system for experimental mouse self-lighting addiction behavior |
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| CN201821271909.0U CN209808619U (en) | 2018-08-08 | 2018-08-08 | Detection system for experimental mouse self-lighting addiction behavior |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111616683A (en) * | 2020-06-08 | 2020-09-04 | 连庆泉 | Experimental rat combined optogenetics addiction behavior detection system |
| CN113141766A (en) * | 2021-04-16 | 2021-07-20 | 中国科学院深圳先进技术研究院 | Electrophysiological experiment shielding box |
| CN113575442A (en) * | 2021-06-07 | 2021-11-02 | 重庆医科大学附属第一医院 | Photophobia ethology detection system for big and small mouse animals |
| CN114190298A (en) * | 2021-12-13 | 2022-03-18 | 复旦大学 | Method for detecting spatial and environmental memory capacity of mouse under negative emotion |
| WO2023109642A1 (en) * | 2021-12-13 | 2023-06-22 | 深圳先进技术研究院 | Animal model for anxiety disorder in large animal |
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2018
- 2018-08-08 CN CN201821271909.0U patent/CN209808619U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111616683A (en) * | 2020-06-08 | 2020-09-04 | 连庆泉 | Experimental rat combined optogenetics addiction behavior detection system |
| CN113141766A (en) * | 2021-04-16 | 2021-07-20 | 中国科学院深圳先进技术研究院 | Electrophysiological experiment shielding box |
| CN113141766B (en) * | 2021-04-16 | 2022-10-14 | 中国科学院深圳先进技术研究院 | Electrophysiological experiment shielding box |
| CN113575442A (en) * | 2021-06-07 | 2021-11-02 | 重庆医科大学附属第一医院 | Photophobia ethology detection system for big and small mouse animals |
| CN114190298A (en) * | 2021-12-13 | 2022-03-18 | 复旦大学 | Method for detecting spatial and environmental memory capacity of mouse under negative emotion |
| CN114190298B (en) * | 2021-12-13 | 2022-12-27 | 复旦大学 | Method for detecting spatial and environmental memory capacity of mice under negative emotion |
| WO2023109642A1 (en) * | 2021-12-13 | 2023-06-22 | 深圳先进技术研究院 | Animal model for anxiety disorder in large animal |
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