CN209806656U

CN209806656U - Experimental device cultivated in a pot is divided to arbuscular mycorrhizal nursery stock

Info

Publication number: CN209806656U
Application number: CN201920450285.7U
Authority: CN
Inventors: 范晓旭
Original assignee: Heilongjiang University
Current assignee: Heilongjiang University
Priority date: 2019-04-03
Filing date: 2019-04-03
Publication date: 2019-12-20
Anticipated expiration: 2029-04-03

Abstract

The utility model provides an arbuscular mycorrhizal nursery stock divides root test potted plant device to solve prior art can not make clear of AM fungi to plant and soil microorganism's direct action, can not quantify AM fungi's effect efficiency, and then hindered AM fungi to plant and soil microorganism colony influence mechanism probe's problem. The utility model discloses a cultivate the basin, middle baffle, gather the passageway, two sealing plugs, two side shields and two moisturizing pipes, middle baffle setting is in the centre of cultivateing the basin and will cultivate the basin and divide into inoculation room and cultivation room, the several recess that the upper end of middle baffle was equipped with, it sets up in inoculation room to gather the passageway, the both ends of gathering the passageway are provided with the sealing plug respectively, two side shields symmetry set up the both sides at middle baffle, the upper end of every side shield is equipped with the several recess, recess on the side shield and the recess one-to-one on the middle baffle, be provided with the moisturizing pipe in inoculation room and the cultivation room respectively. The utility model is used for the nursery stock is cultivated in a pot.

Description

Experimental device cultivated in a pot is divided to arbuscular mycorrhizal nursery stock

Technical Field

The utility model relates to a nursery stock potting technique, concretely relates to experimental device cultivated in a pot is divided to arbuscular mycorrhizal nursery stock.

Background

Since the AM fungus must be symbiotic with plants to complete the life history of the AM fungus, when the influence of the AM (arbuscular mycorrhizal) fungus on the plant physiology and rhizosphere microflora is researched, the direct effective action of the AM fungus hyphae is difficult to distinguish. At present, the AM fungus and the host plant root system are mixed and exist in the existing arbuscular mycorrhizal seedling culture device, namely, the AM fungus and the host plant root system exist in a seedling culture medium, the direct action of the AM fungus on plants and soil microorganisms cannot be determined by the culture mode, the action efficiency of the AM fungus cannot be quantified, and the influence mechanism research of the AM fungus on the plants and soil microorganism colonies is further hindered.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a solve prior art and can not make clear of AM fungi to plant and soil microorganism's direct action, can not quantify AM fungi's effect efficiency, and then hindered the AM fungi to plant and the problem that the soil microorganism colony influence mechanism was explored, and provide an arbuscular mycorrhizal nursery stock divides root test device cultivated in a pot.

The utility model provides an experimental potted plant device of arbuscular mycorrhizal nursery stock root division, its constitution is including cultivateing the basin, middle baffle, gather the passageway, two sealing plugs, two side shields and two moisturizing pipes, middle baffle setting is in the centre of cultivateing the basin and will cultivate the basin and divide into inoculation room and cultivation room, the several recess that the upper end of middle baffle was equipped with, it sets up in inoculation room to gather the passageway, the both ends of gathering the passageway are provided with the sealing plug respectively, two side shields symmetry set up the both sides at middle baffle, the upper end of every side shield is equipped with the several recess, recess on the side shield and the recess one-to-one on the middle baffle, inoculation room and cultivation are indoor to be provided with the moisturizing pipe.

Compared with the prior art, the utility model following beneficial effect has:

1. the utility model discloses can infect but not contain the host plant root system split charging of AM fungi hypha in the cavity of difference with the host plant root system that contains AM fungi hypha by the AM fungi, study respectively through the physiological index and the microbial domain system to two kinds of different plant root systems, judge the difference between the two, make clear and determine the direct action of AM fungi to plant and soil microorganism, can also quantify the effect efficiency of AM fungi simultaneously.

2. The utility model discloses can keep apart the root system through the recess on middle baffle and the side shield, can form symbiont root system and the rhizosphere environment that does not have AM fungi hypha, wherein the recess on the middle baffle is in order to place and fixed host plant root system, then with the root system average allocation to respectively and naturally arrange the recess on the corresponding side shield in for the root system is the state of extending and not the adherence in the middle of on the baffle.

3. The utility model discloses a moisturizing pipe is supplied water respectively for inoculation room and cultivation room, has guaranteed the normal growth of plant, also avoids inoculation room and cultivation room to produce mutual pollution and interference between handling simultaneously.

4. The utility model discloses a collection passageway can be full of and cultivate the matrix, and the root system of plant and AM fungi's hypha all can freely pass in and out.

Drawings

Fig. 1 is a front view of the present invention;

FIG. 2 is a cross-sectional view C-C of FIG. 1;

fig. 3 is a usage state diagram of the present invention.

Detailed Description

The first embodiment is as follows: the embodiment is described by combining fig. 1 and fig. 2, the embodiment comprises a culture pot 1, a middle baffle 2, a collection channel 3, a support frame 7, two sealing plugs 4, two side baffles 5 and two water replenishing pipes 6, the middle baffle 2 is arranged in the middle of the culture pot 1 and divides the culture pot 1 into a inoculation chamber A and a culture chamber B, a plurality of grooves are arranged at the upper end of the middle baffle 2, the collection channel 3 is arranged in the inoculation chamber A, the sealing plugs 4 are respectively arranged at the two ends of the collection channel 3, rubber rings are arranged at the peripheries of the sealing plugs 6, the sealing performance is increased by the rubber rings, the two side baffles 5 are symmetrically arranged at the two sides of the middle baffle 2, a plurality of grooves are arranged at the upper end of each side baffle 5, the grooves on the side baffles 5 correspond to the grooves on the middle baffle 2 one by one, the water replenishing pipes 6 are respectively arranged in the inoculation chamber A and the culture chamber B, and the, thus, the design is beneficial to the normal growth of the root system. A support frame 7 is arranged between the acquisition channel 3 and the culture pot 1, the support frame 7 is positioned in the middle of the culture pot 1, and the support frame 7 prevents the acquisition channel 5 from middle waist collapse; the grooves on the middle baffle 2 are arranged at equal intervals; the collection channel 3 is a channel formed by a stainless steel net with 20 meshes, and the design enables the root system of the plant and the hypha of the AM fungus to freely enter and exit.

Utilize the utility model discloses a device cultivated in a pot carries out the experimental method of arbuscular mycorrhizal nursery stock root division, see figure 3, the method is as follows:

1. firstly, culturing a host plant 8 to 2-3 weeks by using vermiculite and 0.5 XHoagland's nutrient solution;

2. then carefully taking the host plant 8 out of the vermiculite and placing the host plant in a groove on a middle baffle plate 2 of the device, then manually and evenly distributing the biomass of root systems, and respectively placing each evenly distributed root system in a plurality of grooves on a side baffle plate 5;

3. adding a culture medium (such as soil medium) to the inoculation chamber A, wherein the culture medium contains 2% by mass of AM fungus inoculum including spore, hypha and fine root segment;

4. adding another culture medium (such as soil medium) into the culture chamber B, mixing the same amount of inactivated AM fungus inoculum with the culture medium at 2% by mass ratio, and inactivating at 121 deg.C for 2 hr;

5. when the host plant 8 grows to 3-4 weeks, firstly taking down the sealing plug 6 at any one end, slowly pushing the collector 9 into the collecting channel 3, extracting the root system and soil in the collecting channel 3, then slowly taking out the collector 9, pushing out the root system and soil in the pipe through a piston in the collector 9, thereby carrying out in-situ collection of the plant root system for infection rate determination (acetic acid ink method) and in-situ collection of rhizosphere soil for research of a microbial plot system; when the infection rate of the AM fungi is as high as 90%, the AM fungi-plant symbiont is successfully constructed; at this time, although no AM fungal hyphae were present in the culture chamber B, the entire root system was in a symbiotic state.

6. The physiological indexes and the microflora of the plant roots in the inoculation room A and the culture room B are researched, and the difference between the physiological indexes and the microflora is considered to be the change caused by the AM fungal hyphae.

Claims

1. A pot culture device for arbuscular mycorrhizal seedling root division test is characterized by comprising a culture pot (1), a middle baffle plate (2), a collection channel (3), two sealing plugs (4), two side baffle plates (5) and two water replenishing pipes (6), wherein the middle baffle plate (2) is arranged in the middle of the culture pot (1) and divides the culture pot (1) into a inoculation chamber (A) and a culture chamber (B), a plurality of grooves are arranged at the upper end of the middle baffle plate (2), the collection channel (3) is arranged in the inoculation chamber (A), the two ends of the collecting channel (3) are respectively provided with a sealing plug (4), the two side baffles (5) are symmetrically arranged on two sides of the middle baffle (2), the upper end of each side baffle (5) is provided with a plurality of grooves, the grooves on the side baffles (5) correspond to the grooves on the middle baffle (2) one by one, and water replenishing pipes (6) are respectively arranged in the inoculation chamber (A) and the culture chamber (B).

2. The arbuscular mycorrhizal seedling rooting test pot culture device according to claim 1, characterized in that: the grooves on the middle baffle (2) are arranged at equal intervals.

3. The arbuscular mycorrhizal seedling root division test pot culture device according to claim 1 or 2, characterized in that: the collection channel (3) is a channel formed by a stainless steel net with 20 meshes.

4. The arbuscular mycorrhizal seedling rooting test pot culture device according to claim 3, characterized in that: the water replenishing pipe (6) is arranged close to the inner wall 1 of the culture basin (1).

5. The arbuscular mycorrhizal seedling root-dividing test pot culture device according to claim 1, 2 or 4, characterized in that: the potting device further comprises a support frame (7), the support frame (7) is arranged between the collection channel (3) and the culture basin (1), and the support frame (7) is located in the middle of the culture basin (1).