CN209770384U

CN209770384U - Conductive probe for detecting glucose concentration information of in-vivo tissue fluid and electrochemical device

Info

Publication number: CN209770384U
Application number: CN201920179547.0U
Authority: CN
Inventors: 聂荣志; 彭波; 储涛; 熊泉; 赵子健; 张广维
Original assignee: Zhejiang Shu Source Intelligent Technology Co Ltd
Current assignee: Zhejiang Shu Source Intelligent Technology Co Ltd
Priority date: 2019-02-01
Filing date: 2019-02-01
Publication date: 2019-12-13
Anticipated expiration: 2029-02-01

Abstract

The utility model discloses a conductive probe and electrochemical device for surveying internal tissue liquid glucose concentration information, including conductive probe and analytical equipment, conductive probe includes at least a set of electrode, and the electrode includes working electrode and counter electrode at least to and the glucose excitation enzyme carrier that meets with working electrode. The analytical device is connected to the electrodes. And injecting glucose exciting enzyme into the dermis of the skin by using the conductive probe, exciting glucose in the tissue fluid, oxidizing the glucose to generate a current signal, transmitting the current signal to an analysis device by using an electrode sensor of the conductive probe, and obtaining the glucose concentration information of the tissue fluid in the dermis of the skin of the part to be detected by the analysis device by analyzing the magnitude of the current signal. The utility model has the advantages of simple method, easy operation and the like.

Description

Conductive probe for detecting glucose concentration information of in-vivo tissue fluid and electrochemical device

Technical Field

The utility model relates to a conductive probe and electrochemical device for surveying internal tissue fluid glucose concentration information belongs to medical treatment and detects technical field.

Background

At present, glucose concentration levels of diabetes mellitus or hyperglycemia and other abnormal glucose metabolism persons need to be frequently detected to be matched with medicine taking and diet, which is a key link of diabetes mellitus treatment, and whether the glucose levels in vivo are timely monitored and controlled is a decisive factor of the diabetes mellitus treatment.

The glucose to be detected is present in the body in a fluid environment in the body, hereinafter called body fluid, in the form of an aqueous glucose solution. The detection of glucose concentration requires the detection of the glucose signal in the body fluid by an instrument and then the establishment of the relationship between the glucose signal intensity and the glucose concentration by an algorithm.

The detection method is usually in vitro detection, i.e. the liquid such as blood or tissue fluid is transferred to the outside of the body by means of extraction or infiltration, and then the detection is carried out.

Considering the frequent pain and inconvenient operation of in vitro detection of glucose signals and the inability to achieve continuous measurements, methods of in vivo detection of glucose signals have emerged.

Body fluids currently used to measure glucose concentration are intravascular blood, subcutaneous interstitial fluid, and aqueous humor (a type of interstitial fluid) in the eye. The blood-tissue fluid circulation exists, the glucose concentration generally tends to be consistent, however, the glucose concentration change of different body fluids after the sugar intake has difference, and the glucose concentration in the tissue fluid is more closely related to the diabetes treatment in some cases relative to the glucose concentration in the blood, and is more and more regarded by the medical field.

Therefore, there is a need for a better method to overcome the above-mentioned series of drawbacks, which requires: in vivo detection replaces in vitro detection; interstitial fluid glucose signals are detected instead of blood glucose signals.

US6181957 uses optical methods for detecting the aqueous humor of the eye, which contains no blood vessels and avoids the effects of blood signals, however the proposed method has safety issues.

Methods exist for detecting glucose concentration through the skin, even through the skin, such as by passing light through the earlobe or finger, or by puncturing the skin with a needle, however, the tissue at these sites contains a large number of blood vessels, and none of these methods teach how to avoid interference with blood signals, or even recognize the distinction between interstitial fluid and blood.

Current methods ignore the feasibility and necessity of detecting interstitial fluid glucose signals in the dermis. The blood vessels in the dermis are mainly concentrated at the junction of the upper surface and the epidermis and the junction of the lower surface and the subcutaneous tissue, the space structure is formed by protein fibers in the dermis, tissue fluid is filled in the space structure, and if only signals from the interior of the dermis can be collected, the signals from blood can be effectively reduced, and the device is safe and convenient.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a conductive probe and electrochemical device for surveying internal tissue liquid glucose concentration information, through giving the chemical excitation effect to tissue liquid glucose in skin corium, make it produce the current signal to obtain glucose concentration information.

The utility model discloses a following technical scheme realizes:

A conductive probe for detecting the glucose concentration information of tissue fluid in a body can be used for penetrating into the dermis layer of skin, the conductive probe comprises at least one group of electrodes, the electrodes at least comprise a working electrode and a counter electrode, and a glucose kinase carrier connected with the working electrode, and the glucose kinase carrier is used for carrying glucose kinase.

In the above technical solution, the electrode further includes a reference electrode.

In the technical scheme, the conductive probe further comprises a puncture needle and a retention needle arranged outside the puncture needle in a sleeved mode, the retention needle is cylindrical, and the electrode is arranged on the outer wall of the retention needle.

An electrochemical device for detecting glucose concentration information of in-vivo tissue fluid comprises a conductive probe and an analysis device, wherein the conductive probe comprises at least one group of electrodes, the electrodes at least comprise a working electrode and a counter electrode, and a glucose kinase carrier connected with the working electrode, and the glucose kinase carrier is used for carrying glucose kinase; the analysis device is connected to the electrode.

The utility model has the advantages of it is following and beneficial effect: glucose kinase is injected through the conductive probe to enable glucose in interstitial fluid to react to generate current, glucose concentration information is obtained through collecting current analysis, and the operation is simple and reliable.

Drawings

Fig. 1 is a schematic view of the human skin tissue and detection principle of the present invention.

Fig. 2 is a schematic view of a conductive probe according to an embodiment of the present invention.

Fig. 3 is a schematic view of a conductive probe according to another embodiment of the present invention.

Fig. 4 is a schematic diagram of the electrode current test of fig. 3.

in the figure: 1-an analyzer; 2-a conductive probe; 21-a pair of electrodes; 22-a working electrode; 23-glucokinase vectors; 24-a reference electrode; 25-puncture needle; 26-retention needle; 3-a skin layer; 4-dermis layer; 5-subcutaneous tissue.

Detailed Description

The following describes the embodiments and working processes of the present invention with reference to the accompanying drawings.

The terms of orientation such as up, down, left, right, front, and rear in the present specification are established based on the positional relationship shown in the drawings. The corresponding positional relationship may also vary depending on the drawings, and therefore, should not be construed as limiting the scope of protection.

The utility model discloses an electrochemical method for detecting glucose concentration information of tissue fluid in vivo, which uses a conductive probe 2 and an analyzer 1 shown in figure 1 to excite the part to be detected to acquire the glucose concentration information of the tissue fluid.

As shown in fig. 2, the conductive probe 2 includes at least one set of electrodes, i.e., a working electrode 22 and a counter electrode 21, and a glucose kinase carrier 23 connected to the working electrode 22. The glucose kinase can be glucose dehydrogenase or glucose oxidase.

As shown in FIG. 3, the conductive probe 2 may further include a puncture needle 25 and a retention needle 26 fitted over the puncture needle 25. The retention needle 26 is cylindrical, and an electrode is provided on the outer wall of the retention needle 26. The electrodes include three, i.e., a working electrode 22, a counter electrode 21, and a reference electrode 24.

In one embodiment, the conductive probe 2 shown in fig. 3 is selected to include a puncture needle 25 and a retention needle 26 that is disposed outside the puncture needle 25. The retention needle 26 is made of polyester film, and has a length of 1.5mm penetrating the skin, which is 2mm smaller than the thickness of the dermis at the position of the arm. The wall surface of the retention needle 26 is printed with three electrodes, which are a working electrode 22 (W), a counter electrode 21 (C), and a reference electrode 24 (R). Three-electrode sensors incorporate a reference electrode to stabilize the working electrode relative to two-electrode sensors (working and counter electrodes). The graphite is used as a working electrode and a counter electrode, the silver/silver chloride ink is used as a reference electrode, glucose oxidase and potassium ferricyanide are loaded at the lower end of the working electrode and are fixed by polyethylene oxide gel, so that the distribution of the graphite in the direction of the retained needle is less than 1mm, and the graphite only acts in the dermis. The puncture needle 25 and the retention needle 26 are inserted into a body, the puncture needle 25 is pulled out, glucose oxidase on the retention needle 26 starts to excite glucose in tissue fluid, Glucose Oxidase (GOD) catalyzes the oxidation of the glucose in the tissue fluid, potassium ferricyanide is used as an electron mediator to generate gluconic acid and potassium ferrocyanide, and then the potassium ferrocyanide can lose electrons and recover to potassium ferricyanide, and the reaction is accompanied with the gain and loss of electrons to form a current signal. Current flows through the working and counter electrodes and the reference electrode is used to maintain the stability of the potential. As shown in FIG. 4, working electrode 22 and reference electrode 24 are not energized, constitute a fixed voltage electrode potential, and working electrode 22 and counter electrode 21 constitute an energized system that allows measurement of the current passing through the working electrode. By analyzing the magnitude of the current, information on the glucose concentration can be obtained.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A conductive probe for detecting the glucose concentration information of the tissue fluid in the body, which can be used for penetrating into the dermis layer of the skin, characterized in that the conductive probe comprises at least one group of electrodes, the electrodes at least comprise a working electrode (22) and a counter electrode (21), and a glucose kinase carrier (23) connected with the working electrode (22), and the glucose kinase carrier (23) is used for carrying glucose kinase.

2. The conductive probe for detecting information on glucose concentration in interstitial fluid in a body according to claim 1, wherein the electrode further comprises a reference electrode (24).

3. The conductive probe for detecting the glucose concentration information of the tissue fluid in the body according to claim 1 or 2, wherein the conductive probe further comprises a puncture needle (25) and an detaining needle (26) sleeved outside the puncture needle (25), and the electrode is arranged on the outer wall of the detaining needle (26).

4. An electrochemical device for detecting glucose concentration information of tissue fluid in a body, characterized in that the electrochemical device comprises a conductive probe (2) and an analysis device (1), the conductive probe (2) comprises at least one group of electrodes, the electrodes at least comprise a working electrode (22) and a counter electrode (21), and a glucose kinase carrier (23) connected with the working electrode (22), the glucose kinase carrier (23) is used for carrying glucose kinase; the analysis device (1) is connected to the electrodes.

5. The electrochemical device for detecting glucose concentration information in interstitial fluid in a body according to claim 4, wherein the electrodes further comprise a reference electrode (24).

6. The electrochemical device for detecting glucose concentration information of tissue fluid in vivo according to claim 4, wherein the conductive probe (2) further comprises a puncture needle (25) and an detaining needle (26) sleeved outside the puncture needle (25), and the electrode is arranged on the outer wall of the detaining needle (26).