CN209752247U - Stainless steel chromatographic column for ganglioside purification - Google Patents

Abstract

The utility model discloses a stainless steel chromatographic column for ganglioside purification, which comprises a column body, the inside of cylinder is provided with a first arc plate and a second arc plate, the cross sections of the first arc plate and the second arc plate are C-shaped structures, a plurality of connecting grooves are equidistantly arranged at the two ends of the first arc plate, arc-shaped bulges matched with the connecting grooves are fixed at the two ends of the second arc plate, and the second arc plate is embedded into the connecting grooves through the arc-shaped bulges and is connected with the first arc plate; through the first arc board and the second arc board of design, can separate the inside silica gel of filling of cylinder for silica gel contacts with first arc board and second arc board, and both can dismantle the separation simultaneously, thereby great improvement the clearance effect, guaranteed the processingquality in later stage, and both when connecting, the arc arch and spread groove and rubber circle and the block rubber of cooperation design can further increase the stability and the leakproofness of connecting.

Description

stainless steel chromatographic column for ganglioside purification

Technical Field

The utility model belongs to the technical field of the column of separating, concretely relates to ganglioside is stainless steel column of separating for purification.

background

The monosialotetrahexosylganglioside sodium is a safe and effective nerve protection and repair medicine, has obvious curative effects on apoplexy, cerebral palsy, Parkinson's syndrome and the like, is mainly extracted from pig brain at present, and is extracted by using a stainless steel chromatographic column in processing so as to obtain required articles.

The existing chromatographic column still has some defects when in use: the existing analytical column needs to be filled with a certain amount of silica gel, and in long-term use, the inner wall of the analytical column is easy to have certain silica gel residue and other stains, and the analytical column is a hollow cylinder without a top and a bottom, cannot be opened and cleaned, and is directly used for cleaning the inner wall of the cylinder, the effect is general, the use efficiency in the later stage cannot be guaranteed, and therefore a certain improvement space exists.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a ganglioside is stainless steel chromatographic column for purification to solve the problem of proposing among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a stainless steel chromatographic column for purifying ganglioside comprises a column body, a first arc plate and a second arc plate are arranged in the column body, the cross sections of the first arc plate and the second arc plate are C-shaped structures, a plurality of connecting grooves are arranged at the two ends of the first arc plate at equal intervals, the two ends of the second arc plate are fixed with arc bulges matched with the connecting grooves in number, the second arc plate is embedded into the connecting grooves through the arc bulges and connected with the first arc plate, a rubber ring is fixed at the included angle of the height difference formed by the arc-shaped bulge and the second arc plate, the end part of the first arc plate is connected with the rubber ring, the top end of the inner side of the connecting groove is provided with a rubber block which is jointed with the top end surface of the arc-shaped bulge, the side edges of the first arc plate and the second arc plate are provided with grooves, limiting protrusions are fixed on the inner wall of the column body, and the column body is connected with the grooves in a sliding mode through the limiting protrusions.

Preferably, the bottom end of the column body is provided with a bottom cover, the top end of the bottom cover is provided with a sealing ring, the sealing ring and the top end surface of the bottom cover are distributed in a circumferential manner, the bottom cover is fixedly connected with the column body through bolts, and the telescopic springs are fixed with the end surface of the column body.

Preferably, a top cover is fixed at the top end of the column body, and the size, shape and structure of the top cover are the same as those of the bottom cover.

Preferably, the top end of the top cover is provided with a connector, and the connector is connected with an external pipeline.

Preferably, the cylinder is a hollow cylinder structure without top and bottom, and the cylinder is a stainless steel component.

Preferably, the inner diameter of the sealing ring is equal to the inner diameter of the column body, and the sealing ring is an elastic material member.

Preferably, the bottom end of the bottom cover is provided with a pipeline, and the pipeline is communicated with the inside of the column body through the bottom cover.

Compared with the prior art, the beneficial effects of the utility model are that:

1. Through the designed first arc plate and the second arc plate, silica gel filled in the cylinder can be separated, so that the silica gel is in contact with the first arc plate and the second arc plate, and the first arc plate and the second arc plate can be detached and separated at the same time, thereby greatly improving the cleaning effect, ensuring the later processing quality, and when the first arc plate and the second arc plate are connected, the arc-shaped bulge and the connecting groove as well as the rubber ring and the rubber block are matched, so that the connection stability and the sealing property can be further improved;

2. Through the sealing ring of design, can be when bottom and cylinder are connected, with the clearance packing between the two, increased the leakproofness of connecting, the expanding spring of cooperation design simultaneously connects both and makes can directly go on in connecting, need not the secondary installation fixed, uses more convenience.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a cross-sectional view of the column of the present invention;

Fig. 3 is an enlarged schematic view of the area a of fig. 2 according to the present invention;

fig. 4 is a partial cross-sectional view of a first arc plate of the present invention;

Fig. 5 is a schematic structural diagram of the base of the present invention.

In the figure: 1. a connector; 2. a cylinder; 3. a bottom cover; 4. a top cover; 5. a groove; 6. a limiting bulge; 7. a first arc plate; 8. connecting grooves; 9. an arc-shaped bulge; 10. a second arc plate; 11. a rubber ring; 12. a rubber block; 13. a seal ring; 14. a telescoping spring.

Detailed Description

the technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1

Referring to fig. 1 to 4, the present invention provides a technical solution: a stainless steel chromatographic column for purifying ganglioside comprises a column body 2, a first arc plate 7 and a second arc plate 10 are arranged inside the column body 2, the cross sections of the first arc plate 7 and the second arc plate 10 are of a C-shaped structure, a plurality of connecting grooves 8 are formed at the two ends of the first arc plate 7 at equal intervals, arc-shaped bulges 9 matched with the connecting grooves 8 in number are fixed at the two ends of the second arc plate 10, the second arc plate 10 is embedded into the connecting grooves 8 through the arc-shaped bulges 9 and is connected with the first arc plate 7, rubber rings 11 are fixed at included angles of height difference formed by the arc-shaped bulges 9 and the second arc plate 10, the end part of the first arc plate 7 is connected with the rubber rings 11, a rubber block 12 is arranged at the top end surface of the connecting grooves 8, the rubber block 12 is attached to the top end surface of the arc-shaped bulges 9, grooves 5 are formed at the side edges of the first arc plate 7 and the second arc plate 10, a limiting bulge 6 is fixed on the inner wall of the, cylinder 2 is through spacing arch 6 and 5 sliding connection of recess, first arc board 7 and second arc board 10 through the design, can separate the silica gel of 2 inside packings of cylinder, make silica gel contact with first arc board 7 and second arc board 10, both can dismantle the separation simultaneously, thereby great improvement the clearance effect, the processingquality in later stage has been guaranteed, and both when connecting, the protruding 9 of arc and spread groove 8 and rubber circle 11 and the block rubber 12 of cooperation design, can further increase the stability and the leakproofness of connecting.

Example 2

Referring to fig. 1 to 5, the present invention provides a technical solution: a stainless steel chromatographic column for purifying ganglioside comprises a column body 2, a first arc plate 7 and a second arc plate 10 are arranged inside the column body 2, the cross sections of the first arc plate 7 and the second arc plate 10 are of a C-shaped structure, a plurality of connecting grooves 8 are formed at the two ends of the first arc plate 7 at equal intervals, arc-shaped bulges 9 matched with the connecting grooves 8 in number are fixed at the two ends of the second arc plate 10, the second arc plate 10 is embedded into the connecting grooves 8 through the arc-shaped bulges 9 and is connected with the first arc plate 7, rubber rings 11 are fixed at included angles of height difference formed by the arc-shaped bulges 9 and the second arc plate 10, the end part of the first arc plate 7 is connected with the rubber rings 11, a rubber block 12 is arranged at the top end surface of the connecting grooves 8, the rubber block 12 is attached to the top end surface of the arc-shaped bulges 9, grooves 5 are formed at the side edges of the first arc plate 7 and the second arc plate 10, a limiting bulge 6 is fixed on the inner wall of the, cylinder 2 is through spacing arch 6 and 5 sliding connection of recess, first arc board 7 and second arc board 10 through the design, can separate the silica gel of 2 inside packings of cylinder, make silica gel contact with first arc board 7 and second arc board 10, both can dismantle the separation simultaneously, thereby great improvement the clearance effect, the processingquality in later stage has been guaranteed, and both when connecting, the protruding 9 of arc and spread groove 8 and rubber circle 11 and the block rubber 12 of cooperation design, can further increase the stability and the leakproofness of connecting.

In this embodiment, it is preferred, the bottom of cylinder 2 is provided with bottom 3, the top of bottom 3 is provided with sealing ring 13, sealing ring 13 is personally submitted the circumference with the top of bottom 3 and is described the distribution and have a plurality of expanding spring 14, bottom 3 passes through bolt fixed connection with cylinder 2, expanding spring 14 is fixed with the terminal surface of cylinder 2, sealing ring 13 through the design, can be when bottom 3 is connected with cylinder 2, pack the clearance between the two, the leakproofness of connection has been increased, the expanding spring 14 of cooperation design simultaneously, connect both and make and directly go on in connecting, it is fixed to need not the secondary installation, it is more convenient to use.

The utility model discloses a theory of operation and use flow: the utility model discloses in will 100 when using pack 200 meshes silica gel into cylinder 2, the pig brain crude extract is upper a kind with the mobile phase after dissolving, elute with the mobile phase, collect the eluant, will only contain GM1 eluant and contain back end impurity eluant to collect respectively and concentrate to basically there is not organic solvent afterwards, obtain crude concentrate and impurity concentrate;

before silica gel is filled, the first arc plate 7 and the second arc plate 10 are fixedly connected, in the fixing process, the arc-shaped bulge 9 at the end part of the second arc plate 10 is inserted into the connecting groove 8 formed at the end part of the first arc plate 7, then the first arc plate 7 and the second arc plate are extruded towards the inner side, the end part of the first arc plate 7 is stressed to extrude the rubber ring 11, so that the gap between the first arc plate 7 and the second arc plate 10 is reduced, meanwhile, the rubber block 12 extrudes the arc-shaped bulge 9, so that the arc-shaped bulge 9 is inserted into the connecting groove 8 to be limited and fixed again, then, the hollow cylinder formed by the first arc plate 7 and the second arc plate 10 is placed in the cylinder 2, in the inserting process, the groove 5 formed at the side edge of the first arc plate 7 and the second arc plate 10 slides to the inside of the cylinder 2 from the limiting bulge 6, then, the bottom cover 3 and the top cover 4 are fixed on the cylinder 2, in the fixing process, the sealing ring 13 at the top end part of the, the bottom cap 3 is then screwed onto the end of the cylinder 2, and when subjected to pressure, the extension spring 14 compresses, so that the sealing ring 13 is located in the gap between the cylinder 2 and the bottom cap 3, and the gap between the two is completely sealed, and then the device can be used.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. A ganglioside purifies uses stainless steel chromatographic column, includes cylinder (2), its characterized in that: the cylinder (2) is internally provided with a first arc plate (7) and a second arc plate (10), the cross sections of the first arc plate (7) and the second arc plate (10) are of a C-shaped structure, a plurality of connecting grooves (8) are formed in the two ends of the first arc plate (7) at equal intervals, arc bulges (9) matched with the connecting grooves (8) in number are fixed at the two ends of the second arc plate (10), the second arc plate (10) is embedded into the connecting grooves (8) through the arc bulges (9) and is connected with the first arc plate (7), a rubber ring (11) is fixed at an included angle of the height difference formed by the arc bulges (9) and the second arc plate (10), the end part of the first arc plate (7) is connected with the rubber ring (11), the top end of the connecting groove (8) is provided with a rubber block (12), and the rubber block (12) is attached to the top end surface of the arc bulges (9), the side edges of the first arc plate (7) and the second arc plate (10) are provided with grooves (5), the inner wall of the column body (2) is fixed with limiting protrusions (6), and the column body (2) is connected with the grooves (5) in a sliding mode through the limiting protrusions (6).

2. the stainless steel chromatographic column for ganglioside purification according to claim 1, wherein: the bottom end of the column body (2) is provided with a bottom cover (3), the top end of the bottom cover (3) is provided with a sealing ring (13), the sealing ring (13) and the top end surface of the bottom cover (3) are distributed with a plurality of expansion springs (14) in a circumferential shape, the bottom cover (3) is fixedly connected with the column body (2) through bolts, and the expansion springs (14) are fixed with the end surface of the column body (2).

3. The stainless steel chromatographic column for ganglioside purification according to claim 2, wherein: the top end of the column body (2) is fixed with a top cover (4), and the size, shape and structure of the top cover (4) are the same as those of the bottom cover (3).

4. The stainless steel chromatographic column for ganglioside purification according to claim 3, wherein: the top of top cap (4) is provided with connector (1), connector (1) and outside pipe connection.

5. The stainless steel chromatographic column for ganglioside purification according to claim 3, wherein: the cylinder (2) is a hollow cylinder structure without a top and a bottom, and the cylinder (2) is a stainless steel component.

6. The stainless steel chromatographic column for ganglioside purification according to claim 2, wherein: the inner diameter of the sealing ring (13) is equal to that of the column body (2), and the sealing ring (13) is an elastic material component.

7. The stainless steel chromatographic column for ganglioside purification according to claim 3, wherein: the bottom end of the bottom cover (3) is provided with a pipeline which is communicated with the inside of the column body (2) through the bottom cover (3).