CN209707380U - Coherent anti-stokes raman scattering microscope imaging device - Google Patents
Coherent anti-stokes raman scattering microscope imaging device Download PDFInfo
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- CN209707380U CN209707380U CN201920402701.6U CN201920402701U CN209707380U CN 209707380 U CN209707380 U CN 209707380U CN 201920402701 U CN201920402701 U CN 201920402701U CN 209707380 U CN209707380 U CN 209707380U
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Abstract
A kind of coherent anti-stokes raman scattering microscope imaging device, comprising: laser light source, 2-D vibration mirror component, the first optical filter, object lens, displacement platform, collector and data processing mould group；Laser light source is for generating first laser beam and second laser beam；First laser beam is collinearly exported with second laser beam；The optical path of first laser beam and second laser beam is adjusted in first laser beam, second laser beam incidence 2-D vibration mirror component, 2-D vibration mirror component；Leave the first laser beam of 2-D vibration mirror component, second laser beam passes sequentially through the first optical filter, object lens；Object lens focus on first laser beam, second laser beam on displacement platform；For the signal light generated on displacement platform by object lens, collector generates primary data according to signal light, and primary data is exported to data processing mould group；It avoids the need for carrying out the single wavelength laser beam that laser exports light splitting and wavelength adjustment reduces volume, be conducive to business development to improve compactedness.
Description
Technical field
The utility model relates to optical microscopy imaging technologies, aobvious more particularly to a kind of coherent anti-stokes raman scattering
Micro mirror imaging device.
Background technique
Optical microscopy imaging system is due to having higher spatial resolution, in materialogy field and biomedicine
Field is widely used.In recent years, the coherent anti-stokes raman scattering microscope based on intramolecule vibration characteristics
(CoherentAnti-StokesRamanScatteringMicroscope, abbreviation CARSMicroscope) due to without
The advantages that fluorescence probe, high sensitivity, is increasingly becoming the powerful of molecular studies.Please refer to Figure of description Fig. 4, CARS mistake
Journey is based on third-order nonlinear optical effect, is the nonlinear optical process of a four-wave mixing.Generally use two beam center frequencies
Different femtosecond/Ps Laser Pulses are respectively as pump light (ωP) and stokes light (ωS) Lai Jifa sample point
Sub-key resonance, when the difference on the frequency (ω vib) of two-beam is consistent with the eigentone of molecule in sample, molecule is consolidated
There is vibration frequency that can obtain resonant check, then in detection light (ωPR) under the action of generate Anti-Stokes signal (ωCARS)。
Traditional coherent anti-stokes raman scattering microscopy imaging system is using near-infrared Ti:Sapphire laser pulse laser as laser
Source, figure is more huge, and optical path is complex, is unfavorable for the business development and application of the technology.
Utility model content
Based on this, it is necessary to provide a kind of compact-sized coherent anti-stokes raman scattering microscope imaging device.
A kind of coherent anti-stokes raman scattering microscope imaging device, comprising: laser light source, 2-D vibration mirror component,
First optical filter, object lens, displacement platform, collector and data processing mould group；The laser light source for generate first laser beam and
Second laser beam；The first laser beam is collinearly exported with the second laser beam；The first laser beam, the second laser
The incident 2-D vibration mirror component of beam, optical path of the 2-D vibration mirror component to the first laser beam and the second laser beam
It is adjusted；Leave the first laser beam of the 2-D vibration mirror component, the second laser beam passes sequentially through described first
Optical filter, the object lens；The object lens focus on the first laser beam, the second laser beam on the displacement platform；Institute
The signal light is reflexed to the acquisition by the object lens, first optical filter by the signal light generated in rheme moving stage
Device, the collector generates primary data according to the signal light, and the primary data is exported to the data processing mould
Group.
Above-mentioned coherent anti-stokes raman scattering microscope imaging device, by laser light source generate first laser beam,
Second laser beam, first laser beam are collinearly exported with second laser beam, and first laser beam, as pump light, second laser beam is made
It avoids the need for exporting laser single to simplify the light channel structure between laser and object lens for stokes light
Wavelength laser Shu Jinhang light splitting and wavelength adjustment, improve coherent anti-stokes raman scattering microscope imaging device to facilitate
Compactedness, reduce volume, be conducive to business development.
It in one of the embodiments, further include autofocus mechanism, the autofocus mechanism includes focus detection list
Member, the second optical filter and moving assembly；Second optical filter is correspondingly arranged with first optical filter, the object lens respectively；
The first laser beam, the second laser beam through second optical filter by reflexing to described after first optical filter
Object lens；The signal light is by reflexing to first optical filter through second optical filter after the object lens；The focusing inspection
It surveys unit and generates third laser beam；Second optical filter described in the third laser beam transmission；The third laser beam is through the object
Mirror focuses on the displacement platform, and the third laser beam generates reflected light on the displacement platform；The reflected light is described in
The backtracking of third laser beam is to the focus detection unit；The focus detection unit detects the reflected light；
The object lens are mounted on the moving assembly；The moving assembly is according to the mobile institute of the testing result of the focus detection unit
State object lens.
It in one of the embodiments, further include Manual focusing component, the Manual focusing component includes instruction light source, the
One convex lens and CCD camera；The side of the displacement platform is arranged in the instruction light source；The instruction light source generates reference light,
The reference light passes through the object lens and second optical filter；First convex lens focuses the reference light described in entrance
CCD camera.
It in one of the embodiments, further include main casing；The laser light source, the 2-D vibration mirror component, described first
Optical filter, the object lens, the displacement platform, the collector, the autofocus mechanism and Manual focusing component installation
In the main casing.
The autofocus mechanism further includes the first reflecting mirror in one of the embodiments, first reflecting mirror point
It is not correspondingly arranged with second optical filter, the focus detection unit, the third laser beam and the reflected light are described
It is reflected on first reflecting mirror.
Gold or silver are coated on first reflecting mirror in one of the embodiments,.
It in one of the embodiments, further include narrow band filter, the narrow band filter setting filters described first
Between piece and the collector；Narrow band filter described in the signal light transmission, into the collector；The narrow band filter
The first laser beam, the second laser beam are absorbed or reflected.
In one of the embodiments, further include 4F system, the 4F system be in the 2-D vibration mirror component with it is described
Between first optical filter.
It in one of the embodiments, further include adjusting component；The first laser beam, the second laser beam are from described
After laser light source projects, the first laser beam, the second laser beam reflex to the 2-D vibration mirror through the adjusting component
Component.
The adjusting component includes the second reflecting mirror and third reflecting mirror in one of the embodiments, and described first swashs
Light beam, the second laser beam successively reflex to the 2-D vibration mirror group through second reflecting mirror and the third reflecting mirror
Part.
Detailed description of the invention
Fig. 1 is the structure of the coherent anti-stokes raman scattering microscope imaging device of an embodiment of the present invention
Figure；
Fig. 2 is after autofocus mechanism is added in coherent anti-stokes raman scattering microscope imaging device shown in FIG. 1
Structure chart；
Fig. 3 is after Manual focusing component is added in coherent anti-stokes raman scattering microscope imaging device shown in Fig. 2
Structure chart；
Fig. 4 is the microscopical schematic diagram of coherent anti-stokes raman scattering.
Specific embodiment
The utility model will be described more fully below for the ease of understanding the utility model,.But this is practical
It is novel to realize in many different forms, however it is not limited to embodiment described herein.On the contrary, providing these implementations
The purpose of example is to make the understanding of the disclosure of the utility model more thorough and comprehensive.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.
Referring to Fig. 1, the coherent anti-stokes raman scattering microscope imaging for one embodiment of the utility model fills
100 are set, for the microscopic image information for obtaining sample 800.The coherent anti-stokes raman scattering microscope imaging device
100 include laser light source 21,2-D vibration mirror component 22, the first optical filter 23, object lens 24, displacement platform 25, collector 26 and data
Handle mould group；Laser light source 21 is for generating first laser beam and second laser beam；First laser beam is conllinear with second laser beam
Output；First laser beam is as pump light, and second laser beam is as stokes light；First laser beam, second laser beam are incident
The optical path of first laser beam and second laser beam is adjusted in 2-D vibration mirror component 22,2-D vibration mirror component 22；Leave two dimension
First laser beam, the second laser beam of galvanometer component 22 pass sequentially through the first optical filter 23, object lens 24；First laser beam, second
The first optical filter of laser beam transmission 23；Object lens 24 focus on first laser beam, second laser beam on displacement platform 25；Displacement platform 25
On sample 800 generate signal light under the action of first laser beam, second laser beam, after signal light passes through object lens 24, first
Signal light is reflexed to collector 26 by optical filter 23, and collector 26 generates primary data according to signal light, and primary data is defeated
Out to data processing mould group.
First laser beam, second laser beam are generated by laser light source 21, first laser beam is collinearly defeated with second laser beam
Out, and first laser beam is as pump light, second laser beam as stokes light, thus simplify laser and object lens 24 it
Between light channel structure, avoid the need for carrying out the single wavelength laser beam that laser exports light splitting and wavelength adjustment, thus convenient
The compactedness of coherent anti-stokes raman scattering microscope imaging device 100 is improved, volume is reduced, is conducive to business development.
Referring to Fig. 2, being by object lens 24 that first laser beam, second laser beam is quasi- in a wherein embodiment
It really focuses on the sample 800 on displacement platform 25, coherent anti-stokes raman scattering microscope imaging device 100 is also
Including autofocus mechanism, autofocus mechanism includes focus detection unit 32, the second optical filter 31 and moving assembly；Second filter
Mating plate 31 is correspondingly arranged with the first optical filter 23, object lens 24 respectively；First laser beam, second laser beam pass through the first optical filter 23
Afterwards, object lens 24 are reflexed to through the second optical filter 31；Signal light is by reflexing to the first optical filtering through the second optical filter 31 after object lens 24
Piece 23；Focus detection unit 32 generates third laser beam；The second optical filter of third laser beam transmission 31, and third laser beam is saturating
It penetrates parallel with first laser beam, second laser beam respectively or conllinear after the second optical filter 31；Third laser beam is focused through object lens 24
Onto displacement platform 25, third laser beam generates reflected light on displacement platform 25；Reflected light is arrived along the backtracking of third laser beam
Focus detection unit 32；Focus detection unit 32 detects reflected light；Object lens 24 are mounted on moving assembly；Moving assembly
According to the mobile object lens 24 of the testing result of focus detection unit 32, to adjust the distance between object lens 24 and displacement platform 25.
Specifically, reflected light is formed after 800 surface reflection of sample by third laser beam；By the adjusting to object lens 24,
To make third laser beam that there is good focusing effect on sample 800, simultaneously as third laser beam is filtered in transmission second
It is parallel with first laser beam, second laser beam respectively or conllinear after mating plate 31, after the completion of the adjusting of object lens 24, first laser
Beam, second laser beam can also have good focusing effect on sample 800；Optionally, focus detection unit 32 can be according to anti-
It penetrates light intensity or image definition of light etc. and generates testing result；By the second optical filter 31 respectively with the first optical filter 23,
Object lens 24 are correspondingly arranged, and autofocus mechanism adjusts object lens before laser light source 21 issues first laser beam, second laser beam
24 position, thus realize it is automatic focus and Anti-Stokes signal light coexists, shortens data acquisition time, be conducive to big
The detection of area sample 800, and use process is succinctly time saving, facilitates the use of operator；Further, object lens 24 can be carried out
Long-range programming Control.
It is rational deployment autofocus mechanism in a wherein embodiment, improves coherent anti-Stokes Raman and dissipate
Penetrate the compactedness of microscope imaging device 100, autofocus mechanism further includes the first reflecting mirror 33, the first reflecting mirror 33 respectively with
Second optical filter 31, focus detection unit 32 are correspondingly arranged, and third laser beam and reflected light occur instead on the first reflecting mirror 33
It penetrates；To the position of adjustable focus detection unit 32, coherent anti-stokes raman scattering microscope imaging device 100 is improved
Whole compactedness；Specifically, the first convex lens 42, third optical filter 44 are in focus detection unit 32 and the first reflecting mirror 33
Between, the first convex lens 42 is reflexed to through the first reflecting mirror 33 by the reference light of object lens 24.
In a wherein embodiment, to improve the first reflecting mirror 33 to third laser beam, reflected light or reference light
Reflecting effect is coated with gold or silver on the first reflecting mirror 33；To further increase 33 albedo of the first reflecting mirror, the first reflecting mirror
Special deielectric-coating is also coated on 33.
In a wherein embodiment, for the reception accuracy for improving collector 26, coherent anti-Stokes Raman dissipates
Penetrating microscope imaging device 100 further includes narrow band filter 261, and the setting of narrow band filter 261 is in the first optical filter 23 and acquisition
Between device 26；Signal light transmission narrow band filter 261, into collector 26；Narrow band filter 261 is to first laser beam, second
Laser beam is absorbed or is reflected；To avoid first laser beam, second laser beam from impacting the sampling of collector 26, mention
The quality of the primary data of height output；Specifically, it is coated with deielectric-coating on narrow band filter 261, to realize the high transmission of signal light
The low transmission of rate and pump light and stokes light, in the present embodiment, the OD of narrow band filter 261 6 to 8 it
Between.
In a wherein embodiment, to match the output facula of laser light source 21 with object lens 24, be concerned with this anti-support
Gram this Raman scattering microscope imaging device 100 further includes 4F system 60, and 4F system 60 is in 2-D vibration mirror component 22 and first
Between optical filter 23；It is amplified by output facula of the 4F system 60 to laser light source 21, enables the output light of laser light source 21
The size of spot and the bore of object lens 24 match, so that it is suitable to make to focus resulting spot size size through object lens 24, improve phase
The micro- effect of dry anti-Stokes Raman scattering microscope imaging device 100；4F system 60 includes at least two convex lenses, with
Realize that first laser beam, second laser beam expand and project object lens 24；In the present embodiment, 4F system 60 includes second convex
Lens 61 and third convex lens 62, the focal length of the second convex lens 61 are less than the focal length of third convex lens 62, the second convex lens 61 with
The distance between third convex lens 62 should be the sum of the focal length of the two.
In a wherein embodiment, to make first laser beam, second laser beam accurately enter to 2-D vibration mirror component
22, coherent anti-stokes raman scattering microscope imaging device 100 further includes adjusting component 70；First laser beam, second swash
For light beam after the injection of laser light source 21, first laser beam, second laser beam reflex to 2-D vibration mirror component 22 through adjusting component 70.
In a wherein embodiment, to make first laser beam, second laser beam enter 2-D vibration mirror at a predetermined angle
Component 22, adjusting component 70 includes the second reflecting mirror 71 and third reflecting mirror 72, and first laser beam, second laser beam are successively through the
Two-mirror 71 and third reflecting mirror 72 reflex to 2-D vibration mirror component 22；Coherent anti-stokes raman scattering microscope at
As after fixed laser light source 21, by adjusting the angle of the second reflecting mirror 71, can adjust first in 100 assembling process of device
The reflection point of laser beam and second laser beam on third reflecting mirror 72 can adjust by adjusting the angle of third reflecting mirror 72
First laser beam and second laser beam enter the angle of 2-D vibration mirror component 22；Specifically, the second reflecting mirror 71, third reflecting mirror
72 surface is coated with deielectric-coating, to improve the reflectivity to first laser beam, second laser beam.
Referring to Fig. 3, in a wherein embodiment, for that can be concerned with if necessary by being manually focused adjusting
Anti-Stokes Raman scattering microscope imaging device 100 further includes Manual focusing component, and Manual focusing component includes instruction light
Source 41, the first convex lens 42 and CCD camera 43；Indicate that the side of displacement platform 25 is arranged in light source 41；Indicate that light source 41 generates ginseng
Irradiation, reference light pass through object lens 24 and the second optical filter 31；First convex lens 42 focuses reference light and enters CCD camera 43；In
When being manually focused adjusting, CCD camera 43 obtains the image of the sample 800 on displacement platform 25 according to reference light, debugs people
Member determines whether artificial focusing is adjusted accurate according to the clarity of 800 image of sample；Specifically, instruction light source 41 is white light
LED component；The focal length and installation site and the size of the sensing unit of CCD camera 43 of first convex lens 42 match；Specifically,
The size and resolution ratio of the sensing unit of CCD camera 43 and the resolution ratio of object lens 24 are correspondingly arranged.
It further, is the compactedness for improving coherent anti-stokes raman scattering microscope imaging device 100, it is poly- manually
Burnt component further includes third optical filter 44, and the first convex lens 42, third optical filter 44 are in the optical path of third laser beam, third
Optical filter 44 is between focus detection unit 32 and the first convex lens 42, and third laser beam, reflected light transmit third filter respectively
Mating plate 44；For reference light after the focusing of the first convex lens 42, third optical filter 44 generates reflection to reference light, enters reference light
CCD camera 43, so that the optical path of third laser beam, reflected light and reference light be made to be overlapped.
In a wherein embodiment, coherent anti-stokes raman scattering microscope imaging device 100 further includes master
Shell；Laser light source 21,2-D vibration mirror component 22, the first optical filter 23, object lens 24, displacement platform 25, collector 26, data processing mould
Group, autofocus mechanism and Manual focusing component are mounted in main casing；To which significant components or mechanism provide effective protection.
Specifically, using wind cooling temperature lowering, to improve compactedness, laser light source 21 be femtosecond-pulse fiber laser or picosecond
Pulse optical fiber；In a kind of wherein embodiment, laser light source 21 uses the Dual of Refined Laser company, Germany
Color laser；First laser beam and second laser beam temporally and spatially synchronism output；In the present embodiment,
One laser beam is also as detection light.
In a kind of wherein embodiment, laser light source 21 exports the first laser beam of 800nm as pump light and output
The second laser beam of 1040nm is as stokes light, and the wavelength of obtained signal light is 652nm, and present embodiment may be implemented
To the detection imaging of the substance comprising C-H chemical bond.
In a kind of wherein embodiment, laser light source 21 can be with the first laser of the adjustable 780-900nm of output wavelength
Shu Zuowei pump light, and output 1040nm second laser beam as stokes light, present embodiment may be implemented to comprising
The detection imaging of the substance of C-H and C-D chemical bond.
In a kind of wherein embodiment, laser light source 21 can be with the adjustable 930-1030nm second laser beam of output wavelength
As stokes light and output 780nm first laser beam as pump light, present embodiment may be implemented to comprising C-H with
And the detection imaging of the substance of C-D chemical bond.In the present embodiment, the first optical filter 23, third optical filter 44 are long wave filter
Wave plate, 23 surface of the first optical filter are coated with deielectric-coating, to realize the reflection of long wavelength's higher transmitance, short wavelength, to protect
Card can pass through pump light and stokes light, reflection Anti-Stokes signal light；Second optical filter 31 is shortwave filter plate, the
Two optical filters, 31 surface is coated with deielectric-coating, to realize first laser beam, second laser beam and the reflection of signal light, while realizing the
Three laser beams, reflected light and reference light pass through；The Wavelength distribution of third laser beam and the second optical filter 31, third optical filter 44
Match.
Sample 800 is placed on displacement platform 25；By the movement of displacement platform 25, sample 800 can be moved to 2-D vibration mirror
In the scanning range of component 22, displacement platform 25 can adjust scanning range according to 800 size of sample and practical application request；Into one
Step ground, can carry out long-range programming Control to displacement platform 25；The mirror surface size of 2-D vibration mirror component 22 should be greater than laser light source 21
Spot size, and scanning angle can be adjusted according to 800 scanning range of sample, 2-D vibration mirror component 22 to first laser beam,
The optical path of second laser beam is adjusted, and enables first laser beam, second laser beam that can transmit into 800 surface scan of sample
Different parts, collector 26 generates the corresponding primary data of 800 different parts of sample after two-dimensional scanning component adjusts every time.
Specifically, collector 26 is photomultiplier tube, and has supersaturated closing function and manually opened and remotely open
Open function；The collected primary data of collector 26 is integrated into complete image information by data processing mould group, in present embodiment
In, data processing mould group is computer.
In the present embodiment, first laser beam, second laser beam, first laser beam and second laser are generated by laser light source
Beam collinearly exports, and first laser beam is as pump light, second laser beam as stokes light, thus simplify laser with
Light channel structure between object lens avoids the need for carrying out the single wavelength laser beam that laser exports light splitting and wavelength adjustment, from
And facilitate the compactedness for improving coherent anti-stokes raman scattering microscope imaging device, volume is reduced, is conducive to business and opens
Hair.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it cannot be understood as the limitations to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (10)
1. a kind of coherent anti-stokes raman scattering microscope imaging device characterized by comprising laser light source, two dimension
Galvanometer component, the first optical filter, object lens, displacement platform, collector and data processing mould group；The laser light source is for generating first
Laser beam and second laser beam；The first laser beam is collinearly exported with the second laser beam；It is the first laser beam, described
The incident 2-D vibration mirror component of second laser beam, the 2-D vibration mirror component is to the first laser beam and the second laser
The optical path of beam is adjusted；Leave the first laser beam of the 2-D vibration mirror component, the second laser beam passes sequentially through
First optical filter, the object lens；The first laser beam, the second laser beam are focused on the displacement by the object lens
On platform；The signal light is reflexed to institute by the object lens, first optical filter by the signal light generated on the displacement platform
Collector is stated, the collector generates primary data according to the signal light, and the primary data is exported to the data
Handle mould group.
2. coherent anti-stokes raman scattering microscope imaging device according to claim 1, which is characterized in that also wrap
Autofocus mechanism is included, the autofocus mechanism includes focus detection unit, the second optical filter and moving assembly；Described second
Optical filter is correspondingly arranged with first optical filter, the object lens respectively；The first laser beam, the second laser beam pass through
After first optical filter, the object lens are reflexed to through second optical filter；The signal light pass through the object lens after through institute
It states the second optical filter and reflexes to first optical filter；The focus detection unit generates third laser beam；The third laser
Beam transmits second optical filter；The third laser beam focuses on the displacement platform through the object lens, the third laser
Beam generates reflected light on the displacement platform；The reflected light along the third laser beam backtracking to the focus detection
Unit；The focus detection unit detects the reflected light；The object lens are mounted on the moving assembly；The shifting
Dynamic component is according to the mobile object lens of the testing result of the focus detection unit.
3. coherent anti-stokes raman scattering microscope imaging device according to claim 2, which is characterized in that also wrap
Manual focusing component is included, the Manual focusing component includes instruction light source, the first convex lens and CCD camera；The instruction light source
The side of the displacement platform is set；The instruction light source generates reference light, and the reference light passes through the object lens and described the
Two optical filters；First convex lens focuses the reference light and enters the CCD camera.
4. coherent anti-stokes raman scattering microscope imaging device according to claim 3, which is characterized in that also wrap
Include main casing；It is the laser light source, the 2-D vibration mirror component, first optical filter, the object lens, the displacement platform, described
Collector, the autofocus mechanism and the Manual focusing component are mounted in the main casing.
5. coherent anti-stokes raman scattering microscope imaging device according to claim 2, which is characterized in that described
Autofocus mechanism further includes the first reflecting mirror, first reflecting mirror respectively with second optical filter, the focus detection
Unit is correspondingly arranged, and the third laser beam and the reflected light reflect on first reflecting mirror.
6. coherent anti-stokes raman scattering microscope imaging device according to claim 5, which is characterized in that described
Gold or silver are coated on first reflecting mirror.
7. coherent anti-stokes raman scattering microscope imaging device according to claim 1, which is characterized in that also wrap
Narrow band filter is included, the narrow band filter is arranged between first optical filter and the collector；The signal light is saturating
The narrow band filter is penetrated, into the collector；The narrow band filter is to the first laser beam, the second laser beam
It is absorbed or is reflected.
8. special according to claim 1 to coherent anti-stokes raman scattering microscope imaging device described in 7 any one
Sign is, further includes 4F system, and the 4F system is between the 2-D vibration mirror component and first optical filter.
9. special according to claim 1 to coherent anti-stokes raman scattering microscope imaging device described in 7 any one
Sign is, further includes adjusting component；The first laser beam, the second laser beam are described after laser light source injection
First laser beam, the second laser beam reflex to the 2-D vibration mirror component through the adjusting component.
10. coherent anti-stokes raman scattering microscope imaging device according to claim 9, which is characterized in that institute
Stating and adjusting component includes the second reflecting mirror and third reflecting mirror, and the first laser beam, the second laser beam successively pass through described
Second reflecting mirror and the third reflecting mirror reflex to the 2-D vibration mirror component.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109765213A (en) * | 2019-03-27 | 2019-05-17 | 威朋（苏州）医疗器械有限公司 | Coherent anti-stokes raman scattering microscope imaging device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109765213A (en) * | 2019-03-27 | 2019-05-17 | 威朋（苏州）医疗器械有限公司 | Coherent anti-stokes raman scattering microscope imaging device |
| US11879780B2 (en) | 2019-03-27 | 2024-01-23 | Weipeng (Suzhou) Medical Devices Co., Ltd. | Coherent anti-Stokes Raman scattering microscope imaging apparatus |
| CN109765213B (en) * | 2019-03-27 | 2024-03-29 | 苏州威邦震电光电技术有限公司 | Coherent anti-stokes raman scattering microscope imaging device |
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