CN209619365U - Simulate the device of cerebrospinal fluid biomechanical environment building organizational project bone tissue - Google Patents

Abstract

The utility model discloses a kind of devices of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue, wherein device includes main control box, cell incubator, culturing room and the connecting silicone pipe and connector that connect main control box and culturing room, the culturing room is placed in cell incubator, the main control box is located above cell incubator, and main control box controls culturing room's mechanical environment by the way that the mechanics parameter of liquid in connecting silicone pipe mounted is arranged.The utility model is for simulating cerebrospinal fluid beating mechanical environment, dynamic cultivation environment can be set by constructing a parameter in vitro, and implementation record experimental data, convenient for probing into the skeletonization and moulding effect and its mechanotransduction mechanism how cerebrospinal fluid beating regulates and controls newborn vertebral plate, the blank of field bioreactor research is made up.

Description

Simulate the device of cerebrospinal fluid biomechanical environment building organizational project bone tissue

Technical field

The utility model belongs to field of biomedicine technology, and in particular to a kind of simulation cerebrospinal fluid biomechanical environment building The device of organizational project bone tissue.

Background technique

Full state chart, semi-vertebral plate excision decompression can efficiently and effectively expand Spinal volume, release neurothlipsis, as facing Bed ordinary operation be widely used to treatment because spinal canal stenosis, spinal cord and nerve root caused by the reasons such as spinal lesion, wound by The diseases such as pressure.For serious spinal canal stenosis, wound and intraspinal tumor etc., laminectomy is still preferred treatment.However The complication such as unstable spine and dura mater adhesions caused by the postoperative Lamina defect of vertebrae plate resection seriously affect its postoperative effect, accelerate ridge Column retrogression increases operating difficulty again, and the above complication can effectively be prevented by repairing Lamina defect, therefore rebuild vertebral plate pole It is important.

It is reached in terms of the importance that dura mater adhesions were rebuild for recovery spinal stability and prevented to vertebral plate both at home and abroad at present A variety of trials are done at common recognition, and in this aspect, such as using autologous bone transplanting, allogenic bone transplantation, simple artificial bone substitution, titanium plate Substitution etc. rebuilds vertebral plate.Although research report above method can effectively restore spinal stability, prevention dura mater adhesions, difficult To avoid many deficiencies existing for the above vertebral plate method for reconstructing, if autologous bone transplanting is drawn materials limited, and there are the area Qu Gu pain, increase Add patient suffering etc.；A large amount of homogeneous allogenic bone transplantations are also easy to produce immunological rejection and cause the propagation of certain diseases；Simple people The degradation of work bone is slow and is difficult to fix, and easily causes new compressing；Titanium plate substitution causes second operation difficulty etc..And we grind early period Study carefully and has proven to using the compound bata-tricalcium phosphate of mesenchymal stem cell or collagen protein sponge bracket building tissue engineered bone, energy Enough successful reconstitution vertebral plates on rabbit Lamina defect model, and newborn vertebral plate can effectively repair backbone posterior column structure, and dura mater is isolated Outer cicatricial tissue.It can be seen that rebuilding vertebral plate using tissue engineering technique physiological has unique advantage.

Although organizational project rebuild vertebral plate advantage sufficiently confirm, the technology in clinical application there are still it is many still It doubts, wherein the most outstanding is that organizational project rebuilds whether vertebral plate can cause new compressing to spinal cord.However analysis previous experiments As a result, one important phenomenon of discovery: spinal cord, but its mechanism will not be oppressed using the newborn vertebral plate of organizational project bone remoulding It is unclear.Pre-stage test result also found that the direction of newborn vertebral plate bone trabecula is consistent with the mechanics direction that cerebrospinal fluid is beaten.Root According to Wolf law: bone trabecula is always along the direction arrangement to meet with stresses repeatedly, thus it is speculated that the duration mechanics that cerebrospinal fluid beating generates Stimulation plays particularly important effect during above-mentioned variation occurs for newborn vertebral plate, as can illustrating the mechanism of action will be group Particularly important theoretical basis is established in the clinical application of weaver's Cheng Chongjian vertebral plate technology.But how related cerebrospinal fluid beats at present Skeletonization and the moulding for regulating and controlling newborn vertebral plate, both at home and abroad there is not yet relevant report.

Cerebrospinal fluid beating is the product of the basic vital movement of animal (heartbeat and breathing), can be divided into arteriopalmus and cause Pulse wave and breathe caused by respiratory wave.Currently about lumbar segment cerebrospinal fluid beating source, there are two types of theoretical: one is lumbar vertebraes For the beating of section cerebrospinal fluid from the beating of blood vessel in spinal cord, another kind is the caused encephalic cerebrospinal fluid beating of cerebral blood vessel beating It is transmitted by cavum subarachnoidale.Current Main Viewpoints think that the beating of lumbar segment cerebrospinal fluid is mainly derived from blood vessel in spinal cord Beating.

Mechanical stimulation has important regulating and controlling effect in regeneration and remodeling process, and such as periodical tension can promote The regeneration and reparation of tendon tissue；Dynamic pressure, static hydraulic pressure, shear stress can promote stem cell to Chondrocyte Differentiation, into And promote the regeneration of cartilaginous tissue；Shear stress has great influence to the morphosis of vascular endothelial cell, growth and function； Static hydraulic pressure and shear stress all have important regulating and controlling effect to the Proliferation, Differentiation of osteoblast.Mechanicalness mechanical signal is to adjust Skeletonization and the indispensable influence factor of moulding are saved, these signals pass through power conduction and FORCE FEEDBACK mechanism in cell and molecular level Incude the functional load that bone is born, stimulates the bone constantly to remold and maintain with this, to cope with the stimulation of external environment.Cause This, cerebrospinal fluid pulsating force equally also can promote skeletonization and the moulding of vertebra bone vertebral lamina.Therefore, external structure cerebrospinal fluid beating mechanics Model is conducive to further probe into regulating and controlling effect and power of the stimulation of cerebrospinal fluid pulsation tension in vertebral plate skeletonization and moulding process Learn transmission mechanism.

The tissue engineering technique applied to bone tissue regeneration field includes self osteoprogenitor cells and three-dimensional porous rack at present The combined use of material.Bone tissue engineer technology includes four parts: osteogenic induction, osteoblast, osteogenic growth factor, specific Mechanical environment.Osteoblast has been widely used in constructing the cytoskeleton structure of mineralising in vitro.But due to osteoblast Maturation is more tended to, the differentiation and proliferation ability relative to osteoprogenitor cells is weakened.In contrast, mesenchymal stem cell has Stronger Proliferation, Differentiation ability.Its bone marrow aspiration liquid that can have drawn from, also may be derived from adipose tissue or Cord blood.It is filled between marrow Matter stem cell has the potential broken up to different cell lines, and it is thin can be divided into osteoblast, cartilage cell, fat cell and flesh Born of the same parents.Its shortcoming is that be not easy in vitro obtain and Proliferation, Differentiation ability can accordingly weaken after subculture in vitro separately.The three-dimensional of osteoprogenitor cells Stereoscopic culture has certain challenge, such as: the nutrition supply of cell inside porous support, oxygen conveying, metabolic waste row Out etc..Ideal cytoskeleton should possess the mechanical performance similar to human normal bone, and be easy to ideal shape Production.Timbering material suitable for manufacturing the cytoskeleton structure of mineralising should have following advantage: sertoli cell attachment；It supports thin Born of the same parents grow along pore network to internal stent；Biodegradable generation and moulding to promote ontology bone.Timbering material constitutes The basic framework of bone tissue engineer, there are many timbering material type currently used for repairing bone defect, are broadly divided into two kinds: natural raw Object derived material, synthetic material.Natural biological derived material is a kind of day handled by physics and chemical method Right biological tissue, one kind are class bone, bone- xenograft and homogeneous allogenic bone etc., are using the bone tissue inorganic part of other individuals as branch Frame material is using method repairing bone defect that is direct or replacing completely；Another kind is sought according to the principle indirectly or partially substituted Look for the substance of similar cell epimatrix.Studying more timbering material at present has: collagen, gelatin, glycosaminoglycan, fibrin There is good biocompatibility Deng, this kind of material, be conducive to the adherency, differentiation, proliferation of mesenchymal stem cell, but its Degradation rate with higher, and degradation speed is fast.In synthetic material, the common timbering material of bone tissue engineer mainly has: (1) Inorganic material-bioceramic class, such as: hydroxyapatite (HA), tricalcium phosphate bioceramic, biological active glass ceramic, two-phase Ca-P ceramic etc.；(2) macromolecule organic synthesis material, such as polyglycolic acid (PGA), the copolymer of polylactic acid (PLA) and the two (PLGA) etc.；(3) composite material, such as compound, compound, the bioceramic/bone morphogenetic protein of HA/ collagen of HA/PLGA (BMP) compound etc..The advantage of synthetic material is to carry out by adjusting the molecular weight, composition ratio and component of material Allotment, deploys the performance of cytoskeleton according to different organizational project demands.It is suitable to be shown according to a large amount of experiment in vivo The ideal bracket aperture diameter of Oesteoblast growth is 200-400 μm.In addition to this, the interconnected hole net of cytoskeleton Network will also be suitable for skeletonization vascularization.Pore structure inside cytoskeleton can influence the distribution of hydrodynamic shear, mechanical stimulus The Proliferation, Differentiation of range and osteoprogenitor cells.Osteoprogenitor cells in cytoskeleton is not only by biophysics sexual stimulus, but also receptor The regulation of interior special Porcine HGF.Many regulatory factors for regulating and controlling osteoblast Osteoblast Differentiation in vitro are available 's.Wherein bone morphogenic proteins (BMPs) and bomeplasty are closely related, they, which are belonged to, is widely present in the intracorporal conversion life of biology Long factor family (TGF-β).Currently, only BMP-2 and BMP-7 are allowed to be applied to clinic.Recombinant human B MP-2 is applied to vertebra Between fusion, fresh fracture of tibia, upper maxillary fracture bone collection operation.Recombinant human B MP-7 is applied to long bone fracture, fracture Disunion, the treatment of intervertebral fusion.

The physics sexual stimulus that may provide for organizational project structure in view of bioreactor is to construct tissue engineered bone not The technology component part that can or lack will become the core of bone tissue engineer technology.Numerous studies personnel have been developed that various each The dynamic 3D bioreactor of sample imitates the original microenvironment of bone tissue, including rolling bottle, rotary wall type container, fluid perfusion be raw Object reactor etc., these bioreactors can give the various forms of mechanicalness mechanical stimulations of osteocyte bracket complex.But It is still stimulated at present without a bioreactor analog cerebrospinal fluid pulsation tension, restores the mechanics micro-loop of vertebral plate skeletonization moulding Border.

Based on above content, for unstable spine and dura mater adhesions etc. caused by the effectively prevention postoperative Lamina defect of vertebrae plate resection The generation of severe complication, regulating and controlling effect and mechanics of the research cerebrospinal fluid pulsation tension stimulation to newborn vertebral plate skeletonization and moulding Transmission mechanism, so it is extremely crucial using tissue engineering technique physiological reconstruction vertebral plate.Therefore, we there is an urgent need to one kind can Dynamic cultivation osteoprogenitor cells and the stimulation of cerebrospinal fluid pulsation tension can be simulated, restore the mechanics microenvironment of vertebral plate skeletonization and moulding Bioreactor makes up the blank of field bioreactor research.

Utility model content

The technical problem to be solved by the present invention is to provide a kind of simulation cerebrospinal fluid biomechanical environment building group weavers The device of journey bone tissue, for simulating cerebrospinal fluid beating mechanical environment, dynamic cultivation can be set by constructing a parameter in vitro Environment, and implementation record experimental data, convenient for probe into cerebrospinal fluid beating how to regulate and control newborn vertebral plate skeletonization and moulding effect and Its mechanotransduction mechanism makes up the blank of field bioreactor research.

In order to solve the above technical problems, the utility model adopts the following technical solutions:

A kind of device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue, including main control box, cell Incubator, culturing room and the connecting silicone pipe and connector that connect main control box and culturing room, the culturing room are placed in cell In incubator, the main control box is located above cell incubator, and main control box is by being arranged in connecting silicone pipe mounted The mechanics parameter of liquid controls culturing room's mechanical environment.

Preferably, the main control box includes main controller module, peristaltic pump drive control module, solenoid-driven control Module, hydraulic acquisition module, touching display screen, power module, the main controller module include STM32F103RCT6 series Single-chip microcontroller, the first decoupling capacitor, the second decoupling capacitor, third decoupling capacitor, the 4th decoupling capacitor, reset button, the first bypass Capacitor, the first pull-up resistor, STC off line download module, the second pull-up resistor, third pull-up resistor, the 4th pull-up resistor, the 5th Pull-up resistor, the first pull down resistor, the second shunt capacitance, the first bridging resistor, the second bridging resistor, third bridge resistance Device, the 4th bridging resistor, the 6th pull-up resistor, the first light emitting diode, the 19th pin connects on STM32F103RCT6 single-chip microcontroller VCC 3.3 is followed by the first decoupling capacitor, and another foot of the first decoupling capacitor meets tieback GND after the 18th pin；32nd pin meets VCC 3.3 are followed by the second decoupling capacitor, and another foot of the second decoupling capacitor meets tieback GND after the 31st pin；After 48th pin meets VCC 3.3 Third decoupling capacitor is connect, another foot of third decoupling capacitor meets tieback GND after the 47th pin；64th pin meets VCC 3.3 and is followed by Four decoupling capacitors, the 4th another foot of decoupling capacitor meet tieback GND after the 63rd pin, reset key S1 the first shunt capacitance in parallel, and one GND is terminated, then connects with the first pull-up resistor R45 and is connected to VCC 3.3, BOOT0 pin is connected to VCC 3.3 and accesses second Bridge resistor and the first bridging resistor, first bridging another termination GND of resistor, BOOT1 pin, which connects, connects the after VCC 3.3 Four bridging resistors and third bridge resistor, and third bridges another termination GND of resistor, and the first light emitting diode anode tap is first Even connect VCC 3.3 after the 6th pull-up resistor R36, LED1 cathode meets GND, and JTAG download interface circuit is by STC off line download module It completes, the 1st pin of STC off line download module connects VCC 3.3, and the 3rd pin connects the second pull-up resistor, and the 5th pin connects in third Pull-up resistor, the 7th pin connect the 4th pull-up resistor, and under the second pull-up resistor, third pull-up resistor, the 4th pull-up resistor, first Pull-up resistor parallel connection is followed by VCC 3.3, and the other end connects the 13rd pin of tieback after the 5th pull-up resistor, and the 9th pin connects the first drop-down electricity Resistance is followed by GND, and the 2nd pin of STC off line download module connects the second shunt capacitance after meeting VCC, and the second shunt capacitance other end connects the 4th Pin, the 8th, 10,12,14,16,18,20 pins connect GND, the 11st, 17,19 pin floating.

Preferably, the peristaltic pump drive module includes three peristaltic pumps and respective drive circuit, wherein first wriggles Pump, the second peristaltic pump, third peristaltic pump are arranged on the right side of main control box front panel, the first peristaltic pump, and the second peristaltic pump is culture Liquid perfusion peristaltic pump, third peristaltic pump are voltage-controlled peristaltic pump processed of beating, and carry connecting silicone pipe respectively in Wriggling Pump Head portion, are used for Culture solution perfusion and cerebrospinal fluid beating simulation.

Preferably, the peristaltic pump driving circuit includes DRV8825 chip, the 4th shunt capacitance, the 5th shunt capacitance, and Six shunt capacitances, the 7th shunt capacitance, the 8th shunt capacitance, the 9th shunt capacitance, the tenth shunt capacitance, the 7th pull-up resistor, 8th pull-up resistor, the 9th pull-up resistor, the tenth pull-up resistor, the first sampling resistor, the second sampling resistor, the second drop-down electricity Resistance, third pull down resistor, the first slide rheostat, the second light emitting diode, third light emitting diode, DRV8825 chip the 1st draw Connect the 2nd pin after the 5th bypass of foot connection, one end connects the 3rd pin after the 6th shunt capacitance and the parallel connection of the 9th pull-up resistor, one end Even the 11st pin, the 4th pin connects the tenth shunt capacitance and meets VCC, another termination GND of the tenth shunt capacitance, and the 11st pin connects the Nine shunt capacitances, the 9th shunt capacitance other end ground connection, the 14th, 28,29 pins all meet GND, the 5th pin and the 7th pin difference Stepper motor A+, A- are connected, is the A coil power supply of motor, B+, B- of the 8th pin and the 10th pin connection stepper motor, for electricity The B coil power supply of machine, the 6th pin connect the second sampling resistor and are followed by GND, and the 9th pin connects the second pull down resistor and is followed by GND, and the 23rd Pin floating does not connect, and the 15th pin meets VCC, and the 4th shunt capacitance one terminates VCC, a termination GND, the first slide rheostat and the Eight shunt capacitances latter termination GND in parallel, the 8th pull-up resistor of another termination are followed by the 12nd pin and the 13rd pin, the 7th pull-up Resistance one terminates VCC, is grounded after 16 pin of termination while 7th shunt capacitance in parallel, the 17th pin connects the tenth pull-up electricity Resistance is followed by the 5, the 19th pin of VCC and meets stepper motor DECAY, the 20th, 21,22 pins be to control direction that stepper motor works, make Can and revolving speed foot, the 24th, 25,26 pins connect 3,4,5 pins of toggle switch respectively, the 27th pin connect the second pull down resistor and Second light-emitting diodes tube cathode, the 18th pin connect third pull down resistor and are followed by third light-emitting diodes tube cathode, the second light-emitting diodes Pipe and third light-emitting diodes tube anode all meet VCC5.

Preferably, the solenoid valve driving module includes solenoid valve and driving circuit for electromagnetic valve, the solenoid-driven mould Block arranges in pairs or groups light-coupled isolation chip PC817 using field-effect tube come drive magnetic valve and guarantee solenoid valve normal work, PC817 chip The 1st pin connection STM32F103RCT6 single-chip microcontroller input pin, the 2nd pin connect the first current-limiting resistance after be grounded, the 4th draws Foot connects the 11st pull-up resistor and is followed by VCC 12, and the 4th pin connects the grid of metal-oxide-semiconductor after the second current-limiting resistance R3 that connects 4th pull down resistor in parallel simultaneously, the 4th pull down resistor other end ground connection, the 3rd pin meet GND, and it is reversed that metal-oxide-semiconductor source electrode connects first Diode is to VCC 24, and drain electrode is directly grounded, and the solenoid valve is in parallel with the first backward dioded.

Preferably, the touching display screen uses resistive touch screen, and serial port circuit is complete using MAX232ACPE chip At, the 1st pin of the chip and indirect tenth bypass capacitor of the 3rd pin, the indirect tenth of the 4th pin and the 5th pin the Two shunt capacitances, the 2nd pin and the 6th pin connect the 13rd shunt capacitance, the 14th shunt capacitance to GND, the 16th pin respectively VCC 5 is met, and connects the 15th shunt capacitance to ground, the 11st pin connects the PA9 foot of STM32F103RCT6 single-chip microcontroller, the 12nd pin The PA10 foot of single-chip microcontroller is connect, the 14th pin connects the 2nd pin of serial ports socket, and the 13rd pin connects the 3rd pin of serial ports socket, serial ports The 5th pin of socket ground connection.

Preferably, the hydraulic acquisition module includes diffused silicon pressure transmitter sensor, as hydrostatic sensor and liquid Pressure acquisition driving circuit, the hydrostatic sensor is embedded in internal stent, can Sensitive Detection pipeline mechanical change, it is described hydraulic Driving circuit J4 terminal is hydraulic acquisition sensor signal input terminal, and the 1st pin connects VCC 24, and the 2nd pin connects STM32F103RCT6 Chip Microcomputer A/D C input pin PA1, meanwhile, it is grounded after the 2nd pin connection third sampling resistor.

Preferably, the power module is made of reduction voltage circuit and AC to DC converter circuit, further, reduction voltage circuit master It to be made of LM2596 chip and ASM1117 chip, the 1st pin connects VCC 24, the 16th bypass electricity on the LM2596 chip Hold and terminate GND with the 17th shunt capacitance latter termination VCC 24 in parallel, one, it is reversed that the 2nd pin of LM2596 chip connects second Diode is followed by GND, and connects an inductance and the 18th shunt capacitance to GND, and diode, inductance and capacitor constitute output end Charging and discharging circuit, the 4th pin of chip are that signal feeds back foot, connect third current-limiting resistance to ground, and connect a sliding variable resistance Device to VCC 5, the output of VCC 5 connect the first filter capacitor to, the 3rd, 5 pins directly meet GND；The 1st pin of chip ASM1117 Connect the 5, the 2nd pin of VCC and meet GND, the 3rd pin is power supply output pin, the second filter capacitor, third filter capacitor parallel connection be followed by Between 1st pin and the 2nd pin, it is filtered for input voltage；4th filter capacitor, after the 19th shunt capacitance is in parallel It is connected between the 3rd pin and the 2nd pin, is filtered for output voltage.J2 terminal connects 220V alternating current, and the 22nd foot meets guarantor Dangerous silk connects the 1st pin of tieback after transformer again, thus can form the input circuit of transformer；The output loop of transformer connects first One bridge rectifier, rectifier bridge are followed by the 20th shunt capacitance and the 20th bypass capacitor in parallel, most terminating output Terminal J1；The wherein alternating current that T1 out-put supply is 24V, the power supply of bridge rectifier output are the later direct current of rectification Source.

Preferably, the cell incubator is set there are four net-like spacer, containing temperature control unit and can be fully sealed； Further, panel is provided with hole on rear side of cell incubator, and is equipped with rubber stopper in tapping, allows the number for selecting to be passed through culture solution Whether amount or gas crossover passage are opened, and while guaranteeing that cell culture chamber is at fully sealed position, allow mould The connecting silicone pipe of quasi- cerebrospinal fluid beating and culture solution circulation can extend into cell incubator and connect culturing room.

Preferably, further, the culturing room is rectangular parallelepiped structure, and up/down panel is transparent material panel, and side is One global facility is made of non-toxic nontransparent material, and respectively there are three apertures for the front and back panel in the side panel, and are equipped with Three No.1 connectors；A connecting silicone pipe for having airstrainer and clips can be connected on the No.1 connector, be Gas crossover passage；Or connection one has the long connecting silicone pipe of clips, is culture solution circulation canal；Further, institute The junction for stating No.1 connector and aperture is equipped with silica gel ring to guarantee good seal, and respectively there are two apertures for left/right panel, internal It is respectively connected with No. two connectors；It is directly connected to sealing-plug at one outside aperture, is connected with silica gel connection inside the aperture on connector Pipe is blank control pipe；There are No. two connectors on the outside of another aperture, is cerebrospinal fluid simulated solution loop head or outlet, inside two Number connector is connected with connecting silicone pipe, for cerebrospinal fluid beating simulation pipe.

Using the utility model have it is following the utility model has the advantages that the utility model compared with prior art, have it is beneficial Effect is: it is added to mechanical environment necessary to bone cell growth during osteocyte culture, also, the mechanical environment Parameter is controllable precise.The flow velocity when frequency and cerebrospinal fluid circulation of the adjustable cerebrospinal fluid beating of the utility model, can So that 6 solenoid valves of multiple parallel independent control.The cerebrospinal fluid for observing different culturing room can be switched on touching display screen Simulated solution pressure condition.Influence factor of the cerebrospinal fluid to bone cell growth situation is had fairly comprehensive consideration by the utility model And quantified controlling or display are accomplished.

Detailed description of the invention

Fig. 1 is a kind of system structure diagram of cerebrospinal fluid bioreactor of the utility model；

Fig. 2 is a kind of entity structure schematic diagram of cerebrospinal fluid bioreactor of the utility model；

Fig. 3 is a kind of cerebrospinal fluid bioreactor culture of the utility model room arranged in parallel；

Fig. 4 is a kind of cerebrospinal fluid bioreactor culture cell structure schematic diagram of the utility model；

Fig. 5 is the utility model main control cabinet systems solutions design frame chart；

Fig. 6 is the master control of the utility model main control box and peripheral circuit schematic diagram；

Fig. 7 is the utility model main control box JTAG download interface circuit diagram；

Fig. 8 is the utility model main control box power supply indicator and BOOT mode selection circuit schematic diagram；

Fig. 9 is the utility model main control box DRV8825 driving circuit figure；

Figure 10 is the utility model main control box driving circuit for electromagnetic valve figure；

Figure 11 is 232 serial port circuit figure of the utility model main control box U5 module；

Figure 12 is the hydraulic acquisition module circuit diagram of the utility model main control box；

Figure 13 is the utility model main control box LM2596 circuit diagram；

Figure 14 is the utility model main control box ASM1117 circuit diagram；

Figure 15 is the utility model main control box AC to DC converter circuit schematic diagram.

The reference numerals are as follows in figure:

1, main control box；2, the first peristaltic pump；3, the second peristaltic pump；4, third peristaltic pump；5, touching display screen；6, believe Number acquisition unit interface；7, bracket；8, reset button；9, cell incubator；10, net-like spacer；11, culturing room；12, culturing room Up/down panel；13, fixed screw；14, No.1 connector；15, No. two connectors；16, connecting silicone pipe；17, left/right panel； 18, front/rear panel；19, solenoid valve；20, sealed cap；21, culture solution storage bottle；22, cerebrospinal fluid circulation bottle；23, hydraulic biography Sensor；24, airstrainer；25, clips；26, gas-guide tube；27, cerebrospinal fluid pulsating canal；28 control tubes；29 adherent growths are thin Born of the same parents；30, sealing nut；31, No.1 sealing ring.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model It clearly and completely describes, it is clear that the embodiments are a part of the embodiments of the present invention, rather than whole implementation Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work The every other embodiment obtained, fall within the protection scope of the utility model.

As shown in Figure 1, a kind of cerebrospinal fluid bioreactor device of the utility model is thin for external parallel dynamic cultivation bone Born of the same parents, the cell obtained after processing is for osseous surgery treatment, vertebra replacement operation etc..The cerebrospinal fluid bioreactor device includes: Main control box 1, cell incubator 9, culturing room 11.

Wherein main control box 1 include main controller module, peristaltic pump drive control module, solenoid-driven control module, Hydraulic acquisition module, touching display screen module, power module.Main controller module includes STM32F103RCT6 series monolithic Machine, the first decoupling capacitor of decoupling capacitor C19, the second decoupling capacitor C24, third decoupling capacitor C18, the 4th decoupling capacitor C7；It is multiple Position button S1, the first shunt capacitance C31, the first pull-up resistor R45；STC off line download module U8, the second pull-up resistor R40, the The three pull-up resistor pull down resistor R44 of R41, the 4th pull-up resistor R42, the 5th pull-up resistor R43, first, the second shunt capacitance C30；First bridging resistor R32, the second bridging resistor R33, third bridging resistor R34, the 4th bridging resistor R35, 6th pull-up resistor R36, the first Light-emitting diode LED 1.

The 19th pin meets VCC 3.3 and is followed by the first decoupling capacitor C19 on STM32F103RCT6 single-chip microcontroller, and another foot of C19 connects Tieback GND after 18th pin；32nd pin meets VCC 3.3 and is followed by the second decoupling capacitor C24, after another foot of C24 connects the 31st pin Tieback GND；48th pin meets VCC 3.3 and is followed by third decoupling capacitor C18, and another foot of C18 meets tieback GND after the 47th pin；The 64 pins meet VCC 3.3 and are followed by the 4th decoupling capacitor C7, and another foot of C7 meets tieback GND after the 63rd pin.Reset key S1 parallel connection Bypass capacitor C31, a termination GND, then connect with the first pull-up resistor R45 and be connected to VCC 3.3.BOOT0 pin is connected to VCC 3.3 simultaneously accesses the second bridging resistor R33 and the first bridging resistor R32, another termination GND of R32.BOOT1 pin connects Connect the 4th bridging resistor R35 and third bridging resistor R34, another termination GND of R34 after VCC 3.3.First light emitting diode LED1 anode tap connects VCC 3.3 after first connecting the 6th pull-up resistor R36, and LED1 cathode meets GND.JTAG download interface circuit is by STC Off line download module U8 is completed, and the 1st pin of U8 connects VCC 3.3, and the 3rd pin connects the second pull-up resistor R40, and the 5th pin connects the Three pull-up resistor R41, the 7th pin connects the 4th pull-up resistor R42, and R40, R41, R42, R44 parallel connection are followed by VCC 3.3, another End connects the 13rd pin of tieback after the 5th pull-up resistor R43.9th pin connects the first pull down resistor R44 and is followed by GND.The 2nd pin of U8 connects Connect second shunt capacitance C30, the C30 other end after VCC and connects the 4th pin.8th, 10,12,14,16,18,20 pins meet GND.The 11,17,19 pin floating.

Peristaltic pump drive module includes three moist Influent Corp. YZ1515X series stepping complete machine peristaltic pumps and corresponding drive Dynamic circuit.Wherein, the first peristaltic pump 2, the second peristaltic pump 3, third peristaltic pump 4 are arranged on the right side of main control box front panel, and first Peristaltic pump 2, the second peristaltic pump 3 are culture solution perfusion peristaltic pump, and third peristaltic pump is voltage-controlled peristaltic pump processed of beating, in Wriggling Pump Head Connecting silicone pipe is carried in portion respectively, for culture solution perfusion and cerebrospinal fluid beating simulation.Peristaltic pump driving circuit includes DRV8825 Chip, the 4th shunt capacitance CC1, the 5th shunt capacitance CC2, the 6th shunt capacitance CC3, the 7th shunt capacitance CC4, the 8th bypass Capacitor CC5, the 9th shunt capacitance CC6, the tenth shunt capacitance CC7, the 7th pull-up resistor CR1, the 8th pull-up resistor CR2, the 9th The pull-up resistor sampling resistor of CR3, the tenth pull-up resistor CR4, first CR5, the second sampling resistor CR6, the second pull down resistor CR7, Third pull down resistor CR8, the first slide rheostat, the second light emitting diode DS1, third light emitting diode DS2.

Connect the 2nd pin after the 5th bypass CC2 of the 1st pin of DRV8825 connection.The pull-up electricity of 6th shunt capacitance CC3 and the 9th CR3 latter section of the 3rd pin of company in parallel is hindered, one end connects the 11st pin.4th pin connects the tenth shunt capacitance CC7 and meets VCC, and CC7 is another One termination GND.11st pin connects the 9th shunt capacitance CC6, CC6 other end ground connection.14th, 28,29 pins all meet GND.5th draws Foot and the 7th pin are separately connected stepper motor A+, A-, are the A coil power supply of motor.8th pin and the 10th pin connect stepping B+, B- of motor are the B coil power supply of motor.6th pin meets the second sampling resistor CR6 and is followed by GND, and the 9th pin connects under second Pull-up resistor is followed by GND, and the 23rd pin floating does not connect.15th pin meets VCC, and the 4th shunt capacitance CC1 mono- terminates VCC, a termination GND.After first slide rheostat and the 8th shunt capacitance CC5 latter termination GND in parallel, the 8th pull-up resistor CR2 of another termination Connect the 12nd pin and the 13rd pin.7th pull-up resistor CR1 mono- terminate VCC, one termination 16 pin while parallel connection the 7th by It is grounded after the capacitor CC4 of road.17th pin meets the tenth pull-up resistor CR4 and is followed by VCC 5.19th pin meets stepper motor DECAY.The 20,21,22 pins are the direction for controlling stepper motor work, enabled and revolving speed foot.24th, 25,26 pins connect dial-up respectively 3,4,5 pins of switch.27th pin meets the second pull down resistor CR7 and the second light emitting diode DS1 cathode.18th pin connects Three pull down resistor CR8 are followed by third light emitting diode DS2 cathode, DS1 and DS2 anode all meets VCC 5.

Solenoid valve driving module is made of SMC company VDW31-5G-3-02 model solenoid valve 19 and driving circuit for electromagnetic valve. Solenoid valve driving module arranges in pairs or groups light-coupled isolation chip PC817 using IRF3205PBF field-effect tube come drive magnetic valve and guarantee electricity Magnet valve works normally.The input pin of the 1st pin connection STM32F103RCT6 single-chip microcontroller of PC817 chip, the 2nd pin connection the It is grounded after one current-limiting resistance R1.4th pin connects the 11st pull-up resistor R2 and is followed by VCC 12, and the 4th pin is limited in series connection second After leakage resistance R3, even the 4th pull down resistor R4 of parallel connection while the grid of metal-oxide-semiconductor, the R4 other end are grounded.3rd pin meets GND. Metal-oxide-semiconductor source electrode meets the first backward dioded D1 to VCC 24, and drain electrode is directly grounded.Solenoid valve is in parallel with D1.

Touching display screen uses the 4th decoupling capacitor C7062 resistive touch screen of TP of the Kunlun on-state, and serial port circuit is adopted It is completed with MAX232ACPE chip.1st pin of chip and the indirect tenth bypass capacitor C25 of the 3rd pin, the 4th pin and The indirect 12nd shunt capacitance C29 of 5th pin, the 2nd pin and the 6th pin meet the 13rd shunt capacitance C27, the 14th respectively Shunt capacitance C28 to GND.16th pin meets VCC 5, and meets the 15th shunt capacitance C26 to ground.11st pin connects PA9 (U1_TX) foot of STM32F103RCT6 single-chip microcontroller, the 12nd pin connect PA10 (U1_RX) foot of single-chip microcontroller, and the 14th pin connects 2nd pin of serial ports socket, the 13rd pin connect the 3rd pin of serial ports socket, the 5th pin of serial ports socket ground connection.

Hydraulic acquisition module includes that the diffused-silicon pressure of Mei Kong company exploitation changes device sensor, as hydrostatic sensor 23 With hydraulic acquisition driving circuit.Hydrostatic sensor is embedded in inside bracket 7, can Sensitive Detection pipeline mechanical change.Hydraulic drive Dynamic circuit, J4 terminal are hydraulic acquisition sensor signal input terminal, and the 1st pin connects VCC 24, and the 2nd pin connects STM32F103RCT6 Chip Microcomputer A/D C input pin PA1, meanwhile, it is grounded after the 2nd pin connection third sampling resistor R39.

Power module is made of reduction voltage circuit and AC to DC converter circuit.Further, reduction voltage circuit is mainly by LM2596 Chip and ASM1117 chip are constituted.The 1st pin meets VCC 24 on LM2596 chip, by the 16th shunt capacitance C12 and the 17th Road capacitor C14 latter termination VCC 24 in parallel, a termination GND.2nd pin of chip meets the second backward dioded D3 and is followed by GND, And meet an inductance and the 18th shunt capacitance C13 to GND.Diode, inductance and capacitor constitute the charging and discharging circuit of output end. 4th pin of chip is that signal feeds back foot, meets third current-limiting resistance R40 to ground, and connects a slide rheostat to VCC 5, VCC 5 output connect the first filter capacitor C10 to, the 3rd, 5 pins directly meet GND.The 1st pin of chip ASM1117 meets VCC 5, the 2nd pin meets GND, and the 3rd pin is power supply output pin.Second filter capacitor C16, third filter capacitor C17 parallel connection be followed by Between 1st pin and the 2nd pin, it is filtered for input voltage.4th filter capacitor C8, the 19th shunt capacitance C15 Parallel connection is followed by between the 3rd pin and the 2nd pin, is filtered for output voltage.J2 terminal connects 220V alternating current, and the 22nd Foot meets fuse F1 and connects the 1st pin of tieback after transformer T1 again, thus can form the input circuit of transformer.The output of transformer Circuit meets a bridge rectifier D1Bridge first, and rectifier bridge is followed by the 20th shunt capacitance C2 and the 21st in parallel Shunt capacitance C3, most terminating output terminal J1.The wherein alternating current that T1 out-put supply is 24V, the power supply of D1Bridge output To rectify later DC power supply.

The utility model can allow for 6 culturing room 11 but be not limited only to 6 culturing room parallel culture experiment simultaneously, culture Room arrangement mode is as shown in figure 3, it is similar to single experimental conditions, and so it will not be repeated.Culturing room 11 is a rectangular parallelepiped structure, training Supporting room up/down panel 12 is non-toxic high molecular transparent material, can observe directly the growing state of cell.Side panel is also by giving birth to Object non-toxic material is made, by respectively setting 10 apertures in culturing room's up/down panel 12, then will by STAINLESS STEEL HEX SOCKET CAP SCREWS 13 Up/down panel is integral with the fixation of lateral plate version.Further, the junction of culturing room's up/down panel 12 and side panel has one Number sealing ring 31 guarantees that the leakproofness of culturing room 11 is good.The junction of fixed screw 13 and culturing room's up/down panel 12 has two Number sealing ring 27 guarantees that the leakproofness of culturing room 11 is good.The front/rear panel 18 of culturing room respectively has 3 apertures, and tapping can be equipped with No.1 connector 14 can connect a gas-guide tube 26 with clips 25 and airstrainer 24 convenient for culturing room 11 and carry out gas Exchange；Or connection connecting silicone pipe 16 carries out culture fluid exchange.If not needing to use the aperture, there is sealed cap 20 can guarantee No.1 connector 14 is sealed.Further, No.1 connector 14 and front/rear panel 18 have No. two sealing rings 27 to guarantee culturing room 11 leakproofness is good.The left/right panel 17 of side panel respectively opens 2 holes, and the inside in hole is equipped with No. two connectors 15, is equipped with two Root connecting silicone pipe is respectively cerebrospinal fluid pulsating canal 27 and static control tube 28.Further, cerebrospinal fluid pulsating canal 27 is culture Room 11 provides cerebrospinal fluid beating mechanical environment, and both ends have installed No. two connectors 15 on the outside and have been connected with control device.Into One step, the cell and biomaterial composites cultivated are fixed on inside fixed bracket 29, guarantee that the sample of culture can be by Uniform stable mechanical stimulation.It can not be connected on the outside of static 28 both ends of control tube, be sealed using sealing nut 30, cerebrospinal fluid is fought Multiple fixed brackets 29 can be placed simultaneously in dynamic pipe 27 and static control tube 28, for cultivating multiple samples.

Specifically, the structure and function of the utility model cerebrospinal fluid bioreactor can be divided into two it is non-interfering complete Closed circulation part, as a cerebrospinal fluid mechanics parameter simulation loop access and a culture solution aloternate path.One brain ridge Mechanics of liquids parameter simulation circulation path, the access are to carry connecting silicone pipe 16 by third peristaltic pump 4, are accessed hydraulic in bracket 7 Sensor 23, then the generation by 19 combined simulation of solenoid valve control cerebrospinal fluid beating active force.Wherein the frequency of the active force, Pressure can carry out relative parameters setting by touching display screen.Then, then by connecting silicone pipe 16 access cerebrospinal fluid circulation bottle 22, there are 3 apertures on 22 lid of cerebrospinal fluid circulation bottle, hole 1 is liquid flowing outlet, and hole 2 is liquid inflow port, and hole 3 is that gas is arranged Outlet, and guarantee to recycle environment asepsis pollution-free whether 3 position of hole is equipped with airstrainer 24, opens.Finally, silica gel connects Adapter tube 16 is outside 9 rear panel aperture of cell incubator access culturing room's left panel, 17 No. two connectors 15 in outside, right panel 17 One connecting silicone pipe 16 of connection of connector 15 of side two again passes through 9 rear panel aperture of cell incubator and is connected to cerebrospinal fluid circulation At 22 holes 2 of bottle, entire cerebrospinal fluid mechanics parameter simulation loop access is completed.

A connecting silicone pipe 16 is connected at culture solution aloternate path, including 21 top opening 1 of culture solution storage bottle, by the One peristaltic pump 2 or the second peristaltic pump 3 carry the connecting silicone pipe 16, and the aperture at rear portion after cell incubator 9 is connected to training The No.1 connector 14 on 11 front panel 18 of room is supported, then the No.1 connector 14 on rear panel 18 is connected into connecting silicone pipe 16, Across 9 rear panel aperture of cell incubator, it is finally coupled to 21 top opening 2 of culture solution storage bottle, completes entire culture solution more Change access.

Cerebrospinal fluid beating bioreactor can simulate the biomechanical environment of cerebrospinal fluid, and different ginsengs can be arranged in vitro Number environment construction organizational project bone tissue, can form the environment culture cell of closed sterile soft and the compound of biomaterial, train During supporting, various parameters can display real-time and can be stored in table form in computer convenient for subsequent analysis, building Organizational project bone tissue have good biomechanical property, biocompatibility and bioactivity, be orthopaedics clinical research with Using the new method of the one kind for providing building tissue engineered bone.The utility model proposes cerebrospinal fluid bioreactor have base This temperature, gas concentration lwevel, humid control, it can be ensured that sterile operating environment, and can be right easily as needed Environmental parameter is configured change.Based on the utility model dynamic cultivation vertebral plate osteoblast in an in vitro environment, can study The influence that the factors such as Beating Rate, the pressure of cerebrospinal fluid grow up for osteocyte, and a kind of signal analysis and processing and number are provided According to store method convenient for later data processing analysis.Complete equipment realizes automatic intelligent control, compares traditional static culture Method has a clear superiority.

When above-mentioned cerebrospinal fluid pulsating device works normally, signal analysis and processing and data save method.

Specifically, acquiring and being transferred to computer end for cerebrospinal fluid experimental signal by data collecting card, physical channel is selected And suitable sample frequency, sampling number are set, maximum input voltage, minimum input voltage.Minimum (big) input voltage and liquid Pressure sensor selection is related；Sample frequency and sampling number have stringenter range of choice according to the size of cerebrospinal fluid Beating Rate. By many experiments, the multiple groups parameter that can preferably collect pressure signal is finally obtained in the utility model.Selection target text Part folder position, convenient for experiment after find data save location.

After the completion of above-mentioned basic setup, start to acquire signal.System is by being fitted to simulation letter for sampled point discrete signal Number, by signal by filtering processing, reduce noise jamming.By the extracting method of wave crest and trough, peak/valley value is shown, it is right In same filtered signal, simultaneously and destination folder preservation is written in real-time display.Also there is signal analysis and processing and data to protect The method of depositing is also equipped with clocking capability, after obtaining local zone time, by being converted to phase to waveform component, i.e. time axis values is obtained Answer runing time.

The another technical solution of the utility model provides a kind of simulation cerebrospinal fluid biomechanical environment building tissue engineered bone The method of tissue is grasped using the device of such as above-mentioned simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue Make, comprising the following steps:

By culturing room, connecting silicone pipe etc. using ethylene oxide adequate disinfection, cerebrospinal fluid circulation bottle and culture solution are stored Bottle is adequately disinfected under high temperature and high pressure environment；

Under gnotobasis, the brackets such as one layer of collagen are smeared on the cerebrospinal fluid beating simulation pipe and blank control pipe of culturing room Material checks whether culturing room's front and back sides plate upper binding head is installed well convenient for the adherent growth of vertebral plate osteoblast, confirmation Appropriate culture solution is put into culturing room afterwards, and top panel is sealed；

Under gnotobasis, with connecting silicone pipe by hydrostatic sensor in main control box upper bracket and cerebrospinal fluid circulation bottle cover Upper hole connection, hydrostatic sensor are connect with third peristaltic pump, and third peristaltic pump is connect with solenoid valve, then connecting silicone pipe is passed through The aperture of cell incubator rear portion connects solenoid valve with No. two connectors on culturing room's left panel, on the plate No.2 connector of the right side Connecting silicone pipe pass through hole 1 on the aperture of cell incubator rear panel and culture solution storage bottle cover and connect, construct cerebrospinal fluid and follow Ring access；

Under gnotobasis, hole on culture solution storage bottle cover is connected with the first peristaltic pump with connecting silicone pipe, by silica gel Connecting tube passes through and connects No.1 connector on the first peristaltic pump and culturing room's front panel after aperture on cell incubator rear panel, together Sample connects No.1 connector and culture solution on culturing room's rear panel after connecting silicone pipe is passed through the aperture of cell incubator rear panel Hole on bottle cover is stored, culture solution circulation path is completed in building；

It is packed into culture solution in culture solution storage bottle, is packed into water in cerebrospinal fluid circulation bottle；

Signal acquisition data line on solenoid valve is connect with main control box signal acquisition unit interface, opens main control box electricity Source master switch presses reset button on main control box, can be to parameters such as cerebrospinal fluid Beating Rate, flow velocitys on touching display screen It is designed, and switches to pressure real-time dynamic display interface, system pressure when can be visually seen simulation cerebrospinal fluid beating Real-time change；Or signal acquisition data line on solenoid valve is connected with capture card, main control box battery main switch is opened, master is pressed Reset button on control cabinet can be designed the parameters such as cerebrospinal fluid Beating Rate, flow velocity on touching display screen, computer end After select file save location, START button on screen is clicked, start to show signal waveform and is saved in destination folder Data.

It should be appreciated that exemplary embodiment as described herein is illustrative and be not restrictive.Although being retouched in conjunction with attached drawing The one or more embodiments for having stated the utility model, it should be understood by one skilled in the art that not departing from by appended In the case where the spirit and scope of the utility model defined by claim, the change of various forms and details can be made.

Claims (10)

1. a kind of device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue, which is characterized in that including master control Case processed, cell incubator, culturing room and the connecting silicone pipe and connector that connect main control box and culturing room, the culturing room It is placed in cell incubator, the main control box is located above cell incubator, and main control box is by being arranged silica gel mounted The mechanics parameter for connecting liquid in pipe controls culturing room's mechanical environment.

2. the device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue according to claim 1, special Sign is that the main control box includes main controller module, peristaltic pump drive control module, solenoid-driven control module, liquid Acquisition module, touching display screen, power module are pressed, the main controller module includes STM32F103RCT6 series monolithic, First decoupling capacitor, the second decoupling capacitor, third decoupling capacitor, the 4th decoupling capacitor, reset button, the first shunt capacitance, the One pull-up resistor, STC off line download module, the second pull-up resistor, third pull-up resistor, the 4th pull-up resistor, the 5th pull-up electricity Resistance, the first pull down resistor, the second shunt capacitance, the first bridging resistor, the second bridging resistor, third bridge resistor, the Four bridging resistors, the 6th pull-up resistor, the first light emitting diode, the 19th pin meets VCC on STM32F103RCT6 single-chip microcontroller 3.3 are followed by the first decoupling capacitor, and another foot of the first decoupling capacitor meets tieback GND after the 18th pin；After 32nd pin meets VCC 3.3 The second decoupling capacitor is connect, another foot of the second decoupling capacitor meets tieback GND after the 31st pin；48th pin meets VCC 3.3 and is followed by Three decoupling capacitors, another foot of third decoupling capacitor meet tieback GND after the 47th pin；64th pin meets VCC 3.3 and is followed by the 4th Coupling capacitor, the 4th another foot of decoupling capacitor connect tieback GND after the 63rd pin, reset key S1 the first shunt capacitance in parallel, a termination GND, then connect with the first pull-up resistor R45 and be connected to VCC 3.3, BOOT0 pin is connected to VCC 3.3 and accesses the second bridging Resistor and the first bridging resistor, first bridging another termination GND of resistor, BOOT1 pin connect connect after VCC 3.3 the 4th across Termination resistor and third bridge resistor, and third bridges another termination GND of resistor, and the first light emitting diode anode tap first connects the Connecting VCC 3.3 after six pull-up resistor R36, LED1 cathode meets GND, and JTAG download interface circuit is completed by STC off line download module, The 1st pin of STC off line download module connects VCC 3.3, and the 3rd pin connects the second pull-up resistor, and the 5th pin connects third pull-up electricity Resistance, the 7th pin connect the 4th pull-up resistor, and the second pull-up resistor, third pull-up resistor, the 4th pull-up resistor, the first drop-down electricity Resistance parallel connection is followed by VCC 3.3, and the other end connects the 13rd pin of tieback after the 5th pull-up resistor, after the 9th pin connects the first pull down resistor GND is met, the 2nd pin of STC off line download module connects the second shunt capacitance after meeting VCC, and the second shunt capacitance other end connects the 4th and draws Foot, the 8th, 10,12,14,16,18,20 pins connect GND, the 11st, 17,19 pin floating.

3. the device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue according to claim 2, special Sign is that the peristaltic pump drive module includes three peristaltic pumps and respective drive circuit, wherein the first peristaltic pump, second Peristaltic pump, third peristaltic pump are arranged on the right side of main control box front panel, the first peristaltic pump, and the second peristaltic pump is that culture solution perfusion is compacted Dynamic pump, third peristaltic pump are voltage-controlled peristaltic pump processed of beating, and carry connecting silicone pipe respectively in Wriggling Pump Head portion, are filled for culture solution Stream and cerebrospinal fluid beating simulation.

4. the device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue according to claim 3, special Sign is that the peristaltic pump driving circuit includes DRV8825 chip, the 4th shunt capacitance, the 5th shunt capacitance, the 6th bypass electricity Hold, the 7th shunt capacitance, the 8th shunt capacitance, the 9th shunt capacitance, the tenth shunt capacitance, the 7th pull-up resistor, the 8th pull-up Resistance, the 9th pull-up resistor, the tenth pull-up resistor, the first sampling resistor, the second sampling resistor, the second pull down resistor, under third Pull-up resistor, the first slide rheostat, the second light emitting diode, third light emitting diode, the 1st pin of DRV8825 chip connection the Connect the 2nd pin after five bypasses, one end connects the 3rd pin after the 6th shunt capacitance and the parallel connection of the 9th pull-up resistor, and one end connects the 11st and draws Foot, the 4th pin connect the tenth shunt capacitance and meet VCC, another termination GND of the tenth shunt capacitance, and the 11st pin connects the 9th bypass electricity Hold, the 9th shunt capacitance other end ground connection, the 14th, 28,29 pins all meet GND, the 5th pin and the 7th pin are separately connected stepping Motor A+, A- are the A coil power supply of motor, and B+, B- of the 8th pin and the 10th pin connection stepper motor, are the B line of motor Circle power supply, the 6th pin connect the second sampling resistor and are followed by GND, and the 9th pin connects the second pull down resistor and is followed by GND, the 23rd pin floating It does not connect, the 15th pin meets VCC, and the 4th shunt capacitance one terminates VCC, a termination GND, the first slide rheostat and the 8th bypass electricity Hold latter termination GND in parallel, the 8th pull-up resistor of another termination is followed by the 12nd pin and the 13rd pin, the 7th pull-up resistor one end It meets VCC, is grounded after 16 pin of termination while the 7th shunt capacitance in parallel, the 17th pin connects the tenth pull-up resistor and is followed by The 5, the 19th pin of VCC meets stepper motor DECAY, the 20th, 21,22 pins be the direction for controlling stepper motor work, it is enabled and Revolving speed foot, the 24th, 25,26 pins connect 3,4,5 pins of toggle switch respectively, the 27th pin connects the second pull down resistor and the second hair Optical diode cathode, the 18th pin connect third pull down resistor and are followed by third light-emitting diodes tube cathode, the second light emitting diode and Three light-emitting diodes tube anodes all meet VCC5.

5. the device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue according to claim 2, special Sign is that the solenoid valve driving module includes solenoid valve and driving circuit for electromagnetic valve, and the solenoid valve driving module uses field Effect pipe collocation light-coupled isolation chip PC817 carrys out drive magnetic valve and guarantees that solenoid valve works normally, and the 1st of PC817 chip is drawn Foot connects the input pin of STM32F103RCT6 single-chip microcontroller, and the 2nd pin is grounded after connecting the first current-limiting resistance, the 4th pin connection the 11 pull-up resistors are followed by the 12, the 4th pin of VCC after the second current-limiting resistance R3 that connects, even in parallel while the grid of metal-oxide-semiconductor 4th pull down resistor, the 4th pull down resistor other end ground connection, the 3rd pin meet GND, and metal-oxide-semiconductor source electrode connects the first backward dioded extremely VCC 24, drain electrode is directly grounded, and the solenoid valve is in parallel with the first backward dioded.

6. the device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue according to claim 2, special Sign is that the touching display screen uses resistive touch screen, and serial port circuit is completed using MAX232ACPE chip, the core The indirect 12nd bypass electricity of 1st pin of piece and indirect tenth bypass capacitor of the 3rd pin, the 4th pin and the 5th pin Holding, the 2nd pin and the 6th pin connect the 13rd shunt capacitance, the 14th shunt capacitance to GND respectively, and the 16th pin meets VCC 5, And the 15th shunt capacitance is connect to ground, the 11st pin connects the PA9 foot of STM32F103RCT6 single-chip microcontroller, and the 12nd pin connects single-chip microcontroller PA10 foot, the 14th pin connects the 2nd pin of serial ports socket, and the 13rd pin connects the 3rd pin of serial ports socket, serial ports socket the 5th Pin ground connection.

7. the device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue according to claim 2, special Sign is that the hydraulic acquisition module includes diffused silicon pressure transmitter sensor, and as hydrostatic sensor and hydraulic acquisition drive Dynamic circuit, the hydrostatic sensor are embedded in internal stent, can Sensitive Detection pipeline mechanical change, the hydraulic-driven circuit J4 terminal is hydraulic acquisition sensor signal input terminal, and the 1st pin connects VCC 24, and the 2nd pin connects STM32F103RCT6 monolithic Machine ADC input pin PA1, meanwhile, it is grounded after the 2nd pin connection third sampling resistor.

8. the device of simulation cerebrospinal fluid biomechanical environment building organizational project bone tissue according to claim 2, special Sign is that the power module is made of reduction voltage circuit and AC to DC converter circuit, further, reduction voltage circuit mainly by LM2596 chip and ASM1117 chip are constituted, and the 1st pin meets VCC 24 on the LM2596 chip, the 16th shunt capacitance and 17th shunt capacitance latter termination VCC24 in parallel, one terminates GND, and the 2nd pin of LM2596 chip connects the second backward dioded It is followed by GND, and connects an inductance and the 18th shunt capacitance to GND, diode, inductance and capacitor constitute the charge and discharge of output end Circuit, the 4th pin of chip are that signal feeds back foot, connect third current-limiting resistance to ground, and connect a slide rheostat to VCC 5, VCC 5 output connect the first filter capacitor to, the 3rd, 5 pins directly meet GND；The 1st pin of chip ASM1117 meets VCC 5, 2nd pin meets GND, and the 3rd pin is power supply output pin, the second filter capacitor, third filter capacitor parallel connection be followed by the 1st pin and Between 2nd pin, it is filtered for input voltage；4th filter capacitor, the 19th shunt capacitance parallel connection are followed by the 3rd drawing It between foot and the 2nd pin, is filtered for output voltage, J2 terminal connects 220V alternating current, and the 22nd foot connects fuse and connects again The 1st pin of tieback after transformer, thus can form the input circuit of transformer；The output loop of transformer connects a bridge-type first Rectification circuit, rectifier bridge are followed by the 20th shunt capacitance and the 20th bypass capacitor in parallel, most terminating output terminal J1；Its The alternating current that middle T1 out-put supply is 24V, the power supply of bridge rectifier output are the later DC power supply of rectification.

9. the dress of -8 any simulation cerebrospinal fluid biomechanical environment building organizational project bone tissues according to claim 1 It sets, which is characterized in that the cell incubator is set there are four net-like spacer, containing temperature control unit and can be fully sealed； Further, panel is provided with hole on rear side of cell incubator, and is equipped with rubber stopper in tapping, allows the number for selecting to be passed through culture solution Whether amount or gas crossover passage are opened, and while guaranteeing that cell culture chamber is at fully sealed position, allow mould The connecting silicone pipe of quasi- cerebrospinal fluid beating and culture solution circulation can extend into cell incubator and connect culturing room.

10. the dress of -8 any simulation cerebrospinal fluid biomechanical environment building organizational project bone tissues according to claim 1 It sets, which is characterized in that further, the culturing room is rectangular parallelepiped structure, and up/down panel is transparent material panel, and side is One global facility is made of non-toxic nontransparent material, and respectively there are three apertures for the front and back panel in the side panel, and are equipped with Three No.1 connectors；A connecting silicone pipe for having airstrainer and clips can be connected on the No.1 connector, be Gas crossover passage；Or connection one has the long connecting silicone pipe of clips, is culture solution circulation canal；Further, institute The junction for stating No.1 connector and aperture is equipped with silica gel ring to guarantee good seal, and respectively there are two apertures for left/right panel, internal It is respectively connected with No. two connectors；It is directly connected to sealing-plug at one outside aperture, is connected with silica gel connection inside the aperture on connector Pipe is blank control pipe；There are No. two connectors on the outside of another aperture, is cerebrospinal fluid simulated solution loop head or outlet, inside two Number connector is connected with connecting silicone pipe, for cerebrospinal fluid beating simulation pipe.