CN108096633A - A kind of cartilage defect repair material and preparation method thereof - Google Patents
A kind of cartilage defect repair material and preparation method thereof Download PDFInfo
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- CN108096633A CN108096633A CN201810025677.9A CN201810025677A CN108096633A CN 108096633 A CN108096633 A CN 108096633A CN 201810025677 A CN201810025677 A CN 201810025677A CN 108096633 A CN108096633 A CN 108096633A
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- chondroconia
- cartilage defect
- repair material
- cartilage
- defect repair
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3612—Cartilage, synovial fluid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
- A61L27/3645—Connective tissue
- A61L27/3654—Cartilage, e.g. meniscus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Biomedical Technology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Transplantation (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Prostheses (AREA)
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Abstract
The invention belongs to medical instruments fields, disclose a kind of cartilage defect repair material and are made of chondroconia and II collagen types.Chondroconia derives from a wealth of sources described in cartilage defect repair material of the present invention, can be not only the chondroconia of autologous or the chondroconia of xenogenic origin, can avoid Repeated Operation, reduce patient trauma.Contain the cartilage cell with repair ability and the extracellular matrix of activity in cartilage defect repair material of the present invention, and the biocompatibility of collagen-based materials is strong, therefore the move to the interior cell of in-migration of the cell or host being proliferated in cartilage defect repair material is easily survived, and is further proliferated.In vitro culture is not required in cartilage defect repair material of the present invention, can be directly used for the reparation at cartilage defect.
Description
Technical field
The invention belongs to medical instruments fields, and in particular to a kind of cartilage defect repair material and preparation method thereof, especially
It is a kind of chondroconia-collagen composite repair materials and preparation method thereof.
Background technology
Cartilage is the main support ingredient of embryo's early stage, and as development of fetus is gradually substituted by bone, substitution process is prolonged always
Continue latter period of birth.It is the fine and close connective organ of specialization, cytoplasm is firm and flexible.In fetus and year children phase,
Cartilaginous tissue is distributed more widely, is gradually replaced later by bone tissue.It in adult, is only dispersed in and is distributed some cartilages, act on according to institute
Locate position and it is different.Such as it is present in the articular surface articular cartilage of bone, plays weight-bearing load in human body, reduces bone friction between joint
Etc. important roles.
Articular cartilage belongs to hyaline cartilage, and surface is smooth, glossy in light blue, it is special to be called cause by a kind of
The basic framework that the collagenous fibres of close connective tissue are formed, this frame are in semi-circular, similar arch goal, and bottom is tightly attached
It on following sclerotin, upper end makes articular cartilage tightly get up with synosteosis without falling down towards articular surface, this structure
Come, while when being under pressure, there can also be fraction of deformation, play the role of compensator or trimmer pressure.Between these fibers, dissipate
Cartilage cell is being dispersed with, cartilage cell is gradually made of from shallow-layer to deep layer flat sample to oval or circular cell, these
Cartilage cell maintains the eubolism of articular cartilage.Articular cartilage does not have innervation, and also without blood vessel, nutritional ingredient is necessary
It is obtained from joint fluid, and its metabolic waste must be also drained into joint fluid, this Nutrition and Metabolism of articular cartilage must pass through
Joint motions make articular cartilage constantly be under pressure stimulation, so joint motions are for maintaining the normal of articular cartilage
Structure plays an important role.
Articular cartilage defect is very common in Orthopedic Clinical, and arthralgia, limitation of activity usually occurs in patient, seriously affects
Patients ' life quality even results in maimed person.Clinical treatment means are various at present, the not same order often developed according to conditions of patients
Section, and select different treatment means.At present for the severe osteoarthritic of cartilage large defect, and expectant treatment is invalid
When, it is contemplated that row prosthetic replacement.Cartilage defect area<1.5cm2, " micro fractures " technology under arthroscope can be selected.It is right
In cartilage defect area>1.5cm2Self osteochondro tissue transplanting can be selected.Autologous transplanting osteochondro tissue, then need
It to draw materials at non-weight bearing position, therefore source is limited, and there is the complication of materials, add operation wound and operating time.
Allosome osteochondral graft is selected, then faces immunological rejection and the risk of transmission, while allosome tissue is in cartilage defect area
The reconstruction process in domain is also relatively slow.
The content of the invention
In view of this, it is an object of the invention to be directed to problems of the prior art, a kind of new cartilage is provided and is lacked
Repair materials and preparation method thereof are damaged, to repair cartilage defect.
Purpose to realize the present invention, the present invention adopt the following technical scheme that.
A kind of cartilage defect repair material, is made of chondroconia and typeⅡ Collagen.
Wherein, the chondroconia includes cartilage cell, growth factor and extracellular matrix, mainly as seed cell.
The collagen is repair materials matrix, under cryogenic (0-4 DEG C) in dissolved colloidal state, there is mobility, temperature is increased to
(37 DEG C) undergo phase transition in gelatinous, do not have mobility during certain phase；Chondroconia is compound by raising temperature with collagen
Degree undergoes phase transition colloidal sol-chondroconia compound, and so as to which chondroconia is fixed in collagen gel, being formed has a setting
The graft of state and support strength, the cell in chondroconia are survived in collagen and are further proliferated, and reach reparation cartilage
The purpose of defect.
Chondroconia described in cartilage defect repair material of the present invention can be the chondroconia of autologous, also may be used
Think the chondroconia of xenogenic origin.
Preferably, the chondroconia is 1 × 1 × 1mm particles.
Further, preferably, chondroconia and typeⅡ Collagen described in the cartilage defect repair material
Volume ratio be 1:1.
The present invention also provides the preparation method of the cartilage defect repair material, by chondroconia and II under cryogenic conditions
Collagen type is uniformly mixed.
Wherein, the temperature range of the cryogenic conditions is 0-4 DEG C, and container may be employed and be placed in the environment of mixture of ice and water
It obtains, to keep the cytoactive in chondroconia.
In some embodiments, it is described to be mixed into using sterile glass bar stirring mixture.
Further, in some embodiments, the preparation method of the cartilage defect repair material further includes centrifugation
The step of bubble removing.
3D biometric prints mode or other cell-materials may be employed in cartilage defect repair material of the present invention in vitro
Material building mode is handled, and forms the graft with certain form and support strength.
It handles, strikes off bright firstly the need of to damaged cartilage region when being repaired using the graft to cartilage defect
The residual cartilaginous tissue that aobvious regression loosens, bone plate under cartilage-preserving, avoids marrow from largely penetrating into wound repairing.It then will be above-mentioned
Cartilage defect repair material is fixed on cartilage defect region.It will be appreciated by those skilled in the art that fixed form can be biological egg
White gel bonding etc..Suffering limb should be avoided to bear a heavy burden in certain time after prosthetic, but can early stage bend and stretch joint.
As shown from the above technical solution, the present invention provides a kind of cartilage defect repair material by chondroconia and II type glue
Former albumen is made.Chondroconia derives from a wealth of sources described in cartilage defect repair material of the present invention, can be not only self next
The chondroconia in source or the chondroconia of xenogenic origin, can avoid Repeated Operation, to the form of the cartilage defect surface of a wound
Without particular/special requirement, patient trauma is reduced.It is thin to contain the cartilage with repair ability in cartilage defect repair material of the present invention
Born of the same parents and the extracellular matrix of activity, and the biocompatibility of collagen-based materials is strong, therefore be proliferated in cartilage defect repair material thin
The move to the interior cell of in-migration of born of the same parents or host is easily survived, and is further proliferated.Cartilage defect repair material of the present invention is not
In vitro culture is needed, can be directly used for the reparation at cartilage defect.
Description of the drawings
It in order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is attached drawing needed in technology description to be briefly described.
Fig. 1 shows chondroconia figure made from embodiment 1；
Fig. 2 shows 1 chondroconia of embodiment and the mixed chondroconia of II collagen types-collagen complex low temperature
Under the conditions of state diagram；Chondroconia-the collagen complex taken out from ice chest, does not form gel, in mobility；
Fig. 3 shows chondroconia-collagen complex temperature liter that 1 chondroconia of embodiment is mixed with II collagen types
State diagram under the conditions of height to certain phase；After temperature rises to 37 DEG C, in dissolved colloidal state；
Fig. 4 shows the cartilage defect figure of 2 unimplanted chondroconia-collagen-based composite of embodiment, amplification factor 100；
Fig. 5 shows that embodiment 2 injects the cartilage defect figure of chondroconia-collagen-based composite, amplification factor 100.
Specific embodiment
The invention discloses a kind of cartilage defect repair materials.Those skilled in the art can use for reference present disclosure, suitably
Modified technique parameter is realized.In particular, it should be pointed out that all similar substitutions and modifications are for a person skilled in the art
It will be apparent that they are considered as being included in the present invention.The method and product of the present invention is carried out by preferred embodiment
Description, related personnel can substantially not depart from present invention, method described herein are being modified in spirit and scope
Or appropriate change is with combining, to realize and using the technology of the present invention.
For a further understanding of the present invention, below in conjunction with the embodiment of the present invention, to the technical side in the embodiment of the present invention
Case is clearly and completely described, it is clear that described embodiment is only part of the embodiment of the present invention rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art institute without making creative work
The every other embodiment obtained, belongs to the scope of protection of the invention.
Unless otherwise specified, reagent involved in the embodiment of the present invention is commercial product, can pass through business canal
Road purchase obtains.
The preparation of embodiment 1, chondroconia-collagen-based composite.
1st, chondroconia prepares:
The fresh articular cartilage of the childhood human body of donations is carried out cutting process by aseptic condition, is allowed to form 1 × 1 × 1mm
Chondroconia (Fig. 1).Survival detection, cell survival rate 80%-95% before display transplanting.It can be carried out using DMEM culture mediums
Interim preservation or Cryopreservation.
2nd, the preparation of collagen-based materials：
Intercept the articular cartilage 200g (weight in wet base) of healthy adult pig, through 0-10 DEG C lyophilized, crushing, aseptic process, digestion, salt
Analysis, dialysis (referring to《The compound autologous bone marrow mesenchymal stem cells of Acellular bone cartilage frame repair the research of sheep bone cartilage defect》.
Chinese joint surgery magazine (electronic edition), 06 phase in 2010:765-771.) typeⅡ Collagen 10g is made.After Sterility testing
It can apply.
3rd, the preparation of chondroconia-collagen-based composite
By chondroconia and II collagen types according to 1 under the conditions of 0-4 DEG C：1 volume ratio is uniformly mixed, and degasification is gone in centrifugation
It is for use (Fig. 2, Fig. 3) to be placed in ice chest for bubble.
Embodiment 2,
Pig cartilage defect is modified, makes residual cartilaginous edges smooth, stable, non-loosening.Cartilage made from embodiment 1 is micro-
Grain-collagen-based composite injects cartilage defect region under aseptic conditions, and rise implanted region temperature maintains 6-10min to 37 DEG C,
Colloidal sol host material mutually becomes gel.Flexion-extension motion operation joint, observation graft do not fall off in cartilage defect region, that is, complete
Implant surgery.
It performs the operation the 3-6 months, takes out sample and carry out the analysis of II Collagen Type VIs immunohistochemical staining, the result is shown in Figure 1 and Fig. 2.
The results show that the cartilage defect of unimplanted chondroconia-collagen-based composite, cartilage defect is apparent (Fig. 1)；And
The cartilage defect of injection chondroconia-collagen-based composite shows that defect cell well sticks increasing on collagen-based materials surface
It grows, cartilage defect obtains significantly repairing (Fig. 2).
Claims (5)
1. a kind of cartilage defect repair material, is made of chondroconia and typeⅡ Collagen.
2. cartilage defect repair material according to claim 1, which is characterized in that the chondroconia is 1 × 1 × 1mm
Particle.
3. cartilage defect repair material according to claim 1, which is characterized in that the chondroconia and II Collagen Type VI egg
White volume ratio is 1:1.
4. the preparation method of cartilage defect repair material described in claim 1, which is characterized in that by chondroconia under cryogenic conditions
It is uniformly mixed with II collagen types.
5. preparation method according to claim 4, which is characterized in that the temperature range of the cryogenic conditions is 0-4 DEG C.
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| CN201810025677.9A CN108096633A (en) | 2018-01-11 | 2018-01-11 | A kind of cartilage defect repair material and preparation method thereof |
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| CN201810025677.9A CN108096633A (en) | 2018-01-11 | 2018-01-11 | A kind of cartilage defect repair material and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109893301A (en) * | 2019-04-23 | 2019-06-18 | 上海市第六人民医院 | A kind of transplant and method for repairing articular cartilage defect |
| CN114340686A (en) * | 2020-02-28 | 2022-04-12 | 爱恩斯生物科技(昆山)有限公司 | Cartilage regeneration composition containing cartilage component and its preparation method |
| CN115920134A (en) * | 2022-12-19 | 2023-04-07 | 成都奇璞生物科技有限公司 | Cartilage microparticles, cartilage scaffold and preparation method thereof |
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2018
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109893301A (en) * | 2019-04-23 | 2019-06-18 | 上海市第六人民医院 | A kind of transplant and method for repairing articular cartilage defect |
| CN114340686A (en) * | 2020-02-28 | 2022-04-12 | 爱恩斯生物科技(昆山)有限公司 | Cartilage regeneration composition containing cartilage component and its preparation method |
| CN114340686B (en) * | 2020-02-28 | 2023-06-06 | 爱恩斯生物科技(昆山)有限公司 | Cartilage regeneration composition containing cartilage component and method for producing the same |
| CN115920134A (en) * | 2022-12-19 | 2023-04-07 | 成都奇璞生物科技有限公司 | Cartilage microparticles, cartilage scaffold and preparation method thereof |
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