CN209602548U - A kind of culture systems - Google Patents
A kind of culture systems Download PDFInfo
- Publication number
- CN209602548U CN209602548U CN201920153341.0U CN201920153341U CN209602548U CN 209602548 U CN209602548 U CN 209602548U CN 201920153341 U CN201920153341 U CN 201920153341U CN 209602548 U CN209602548 U CN 209602548U
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- Prior art keywords
- sink
- pipe
- bottle cap
- culture
- culture dish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model proposes a kind of culture systems, comprising: sink, sealing cover, culture dish, four-way bottle cap, air inlet pipe, escape pipe, feed supplement pipe, sample presentation pipe, air-pressure tube, probe tube, sampling bottle and threeway bottle cap；Sealing cover is mounted on above sink for sealed cistern, and sealing cover is equipped with multiple through-holes, and each through-hole is each equipped with corresponding sealing-plug；Culture dish is mounted in sink, and four-way bottle cap matches with culture dish and is mounted on culture dish opening；Sampling bottle disassembly is mounted on sink and is located at outside sink, and threeway bottle cap matches with sampling bottle and is mounted on sampling bottle opening.The utility model proposes a kind of culture systems do not need to open culture dish and sink, so that sampling can carry out in the culture environment of sealing, be conducive to avoid sampling the damage to culture environment when being sampled to culture dish；Meanwhile reducing sampling difficulty.
Description
Technical field
The utility model relates to culture systems technical field more particularly to a kind of culture systems.
Background technique
In the research field of animal, plant and microorganism, culture and indigo plant of the photosynthetic culture systems for certain plants
The culture of bacterium microbe is most important.However in actual incubation, with the existing culture systems analysis used, deposit
In this several disadvantage: 1, due to need provide culture needed for temperature, such as 37 DEG C, such that it is existing open or
The volatilization of moisture in the bad device of air-tightness needs to spend time observation SEA LEVEL VARIATION and moisture is replenished in time；2, moisture
Volatilization or air-tightness not will increase by force working time of heating device, not only shorten its service life and waste electric power resource；
3, since air inlet pipe leads directly to bottom, so that the addition and sampling of culture medium are all very constant, complicated operation；4, this device
Air-tightness design it is not rigorous enough, cannot preferably guarantee that the air-tightness of device is intact；5, snorkel and the problem of air-tightness, increase
The difficulty of sterile working is added.
Utility model content
Technical problems based on background technology, the utility model proposes a kind of culture systems.
The utility model proposes a kind of culture systems, comprising: sink, sealing cover, culture dish, four-way bottle cap, air inlet pipe,
Escape pipe, feed supplement pipe, sample presentation pipe, air-pressure tube, probe tube, sampling bottle and threeway bottle cap；
Sealing cover is mounted on above sink for sealed cistern, and sealing cover is equipped with multiple through-holes, and each through-hole configures
There is corresponding sealing-plug；Culture dish is mounted in sink, and four-way bottle cap matches with culture dish and is mounted on culture dish opening；It takes
Sample bottle disassembly is mounted on sink and is located at outside sink, and threeway bottle cap matches with sampling bottle and is mounted on sampling bottle opening；
Air inlet pipe, escape pipe and feed supplement pipe are installed on four-way bottle cap and stretch out sink through sealing cover；Sample presentation pipe one
End connection four-way bottle cap, the other end connect threeway bottle cap；On air inlet pipe, escape pipe, feed supplement pipe and sample presentation pipe and four-way bottle cap
Four through-hole structures correspond；Air inlet pipe and sample presentation pipe pass through the pipe that four-way bottle cap is connected with insertion culture dish bottom respectively
Road；
Air-pressure tube and probe tube are installed on threeway bottle cap, on sample presentation pipe, air-pressure tube and probe tube and threeway bottle cap
Three through-hole structures correspond, the pipeline that probe tube passes through threeway bottle cap connection insertion sampling bottle bottom；Also pacify on air-pressure tube
Equipped with sterilizing filter；
The first tongs, second are separately installed on air inlet pipe, escape pipe, feed supplement pipe, sample presentation pipe, air-pressure tube and probe tube
Tongs, third tongs, the 4th tongs, the 5th tongs and the 6th tongs.
Preferably, the first fixed frame is installed, sampling bottle disassembly is mounted on the first fixed frame on the outside of sink.
Preferably, the second fixed frame is installed, culture dish disassembly is mounted on the second fixed frame, and culture dish inside sink
There is gap between bottom of gullet and inboard wall of cistern.
Preferably, temperature detection sensor and heating device are also equipped in sink.
Preferably, temperature detection sensor is connected with the display being mounted on outside sink.
Preferably, temperature detection sensor and heating device are located at two sides opposite in sink, and heating device is mounted on water
Trench bottom.
Preferably, temperature detection sensor is connect with heating device, and heating device is used for according to temperature detection sensor
Detected value adjusts water temperature in sink.
Preferably, water circulating pump is also equipped in sink.
Preferably, sealing-plug is nested to form by the rubber ring annular of multiple and different diameters.
Preferably, sealing cover is hingedly mounted on sink, and sealing cover is equipped with multiple U-shaped openings far from the side of shaft, often
One U-shaped opening is equipped with a spill cap liner, and the disassembly of spill cap liner is mounted on sealing cover and sealing-plug is cooperated to block U
Type opening.
The utility model proposes a kind of culture systems so that sampling bottle and culture dish is metastable is installed, from
And it ensure that and make culture dish that it is manual to avoid sampling bottle to the stabilization of sampling bottle sample presentation process and convenient for operation by sample presentation pipe
System caused by fixing or being fixed in such a way that other are separated with sink is unstable.
The utility model proposes a kind of culture systems, when being sampled to culture dish, do not need open culture dish and
Sink is conducive to avoid sampling the damage to culture environment so that sampling can carry out in the culture environment of sealing；Meanwhile it dropping
Low sampling difficulty.
Detailed description of the invention
Fig. 1 be the utility model proposes a kind of culture systems structure chart；
Fig. 2 is the top view formed inside sink in Fig. 1；
Fig. 3 is the top view of sealing cover
Fig. 4 is sealing-plug top view；
Fig. 5 is sealing-plug cross-sectional view；
Fig. 6 is U-shaped opening and sealing-plug Local map on sealing cover；
Fig. 7 is cap liner structure chart.
Diagram:: sink 1, sealing cover 2, culture dish 3, horizontal plane 4, four-way bottle cap 5, air inlet pipe 6, escape pipe 7, feed supplement pipe
8, sample presentation pipe 9, sampling bottle 10, air-pressure tube 11, probe tube 13, threeway bottle cap 14, the first fixed frame 15, U-shaped opening 16, culture dish
Placement region 17, temperature detection sensor 18, water circulating pump 19, heating device 20, sealing-plug 21, cap liner 22.
Specific embodiment
Referring to Fig.1, the utility model proposes a kind of culture systems, comprising: sink 1, sealing cover 2, culture dish 3, four-way
Bottle cap 5, air inlet pipe 6, escape pipe 7, feed supplement pipe 8, sample presentation pipe 9, air-pressure tube 11, probe tube 13, sampling bottle 10 and threeway bottle cap 14.
Sealing cover 2 is mounted on above sink 1 for sealed cistern 1, and sealing cover 2 is equipped with multiple through-holes, and each through-hole is equal
Configured with corresponding sealing-plug 21.Culture dish 3 is mounted in sink 1, and four-way bottle cap 5 matches with culture dish 3 and is mounted on culture
3 opening of ware.In this way, the four-way bottle cap 5 that various pipes can be inserted on sink 1 and culture dish 3 by the through-hole on sealing cover 2 connects
It connects.In present embodiment, the setting of multiple through-holes can meet the demand of the pipe of different-diameter by the through-hole of different-diameter,
So that the culture dish 3 of different size can match the sink 1 work.
The disassembly of sampling bottle 10 is mounted on sink 1 and is located at outside sink 1, and threeway bottle cap 14 matches and pacifies with sampling bottle 10
Mounted in 10 opening of sampling bottle.
Air inlet pipe 6, escape pipe 7 and feed supplement pipe 8 are installed on four-way bottle cap 5 and stretch out sink 1 through sealing cover 2.Into
Tracheae 6 is for connecting feeder, for gas, such as air, oxygen, carbon dioxide needed for supplementing into culture dish 3 etc.；Out
Tracheae 7 avoids the high pressure in culture dish 3 from adversely affecting for the gas discharge in culture dish 3；Feed supplement pipe 8 is used for training
It supports and adds culture medium in ware 3.
9 one end of sample presentation pipe connects four-way bottle cap 5, and the other end connects threeway bottle cap 14.Air inlet pipe 6, escape pipe 7, feed supplement pipe 8
It is corresponded with four through-hole structures on sample presentation pipe 9 and four-way bottle cap 5.Air inlet pipe 6 and sample presentation pipe 9 pass through four-way bottle cap respectively
5 are connected with the pipeline of insertion 3 bottom of culture dish.
In present embodiment, sampling bottle 10 is mounted on 1 outside of sink, so that sampling bottle 10 and culture dish 3 are relatively stable
Carry out install, thus ensure that by sample presentation pipe 9 make culture dish 3 to the stabilization of 10 sample presentation process of sampling bottle and convenient for behaviour
Make, it is unstable to avoid system caused by sampling bottle 10 is fixed manually or is fixed in such a way that other are separated with sink 1
It is fixed.
Specifically, in present embodiment, the first fixed frame 15 is installed on the outside of sink 1, the disassembly of sampling bottle 10 is mounted on the
On one fixed frame 15；Second fixed frame is installed, the disassembly of culture dish 3 is mounted on the second fixed frame, and culture dish inside sink 1
There is gap between 3 and 1 inner wall of 1 bottom of sink and sink.In this way, can be distinguished by the first fixed frame 15 and the second fixed frame
Fast and convenient installation sampling bottle 10 and culture dish 3, complexity of the reduction system with exchanging sample bottle 10 and culture dish 3 for improve
Operating efficiency.
Air-pressure tube 11 and probe tube 13 are installed on threeway bottle cap 14, sample presentation pipe 9, air-pressure tube 11 and probe tube 13 and three
Three through-hole structures on logical bottle cap 14 correspond, and probe tube 13 passes through the connection insertion of threeway bottle cap 14 10 bottom of sampling bottle
Pipeline.Sterilizing filter 12 is also equipped on air-pressure tube 11, to guarantee to pass through air-pressure tube 11 into sampling bottle 10 when input air,
It avoids pollution object and enters sampling bottle 10.
First is separately installed on air inlet pipe 6, escape pipe 7, feed supplement pipe 8, sample presentation pipe 9, air-pressure tube 11 and probe tube 13 to stop
Water presss from both sides A1, the second tongs, third tongs A3, the 4th tongs A4, the 5th tongs A5 and the 6th tongs A6.
Specifically, in present embodiment the pure water of preset temperature can be added in sink 1 to provide conjunction for culture dish 3
Suitable culture environment；Then culture medium is sent into culture dish 3 by feed supplement pipe, and institute is inputted to culture dish 3 by air inlet pipe 6
Gas is needed, to form suitable gaseous environment in culture dish 3.In present embodiment, culture has been isolated by four-way bottle cap 5
Ware 3 and external environment advantageously ensure that the stabilization of culture environment in culture dish 3, avoid pollution；By sealing cover 2 further every
From culture dish 3, be conducive to the safety for further increasing culture experiment.
Specifically, sealing cover 2 is hingedly mounted on sink 1 in present embodiment, to facilitate operation.And present embodiment
In, cooperated in the state of 2 box cover of sealing cover using damascene structures and sink 1, to reduce 1 temporary space of sink, and is improved close
Cover the sealing structure of 2 pairs of sinks 1.
In present embodiment, in incubation, the 4th tongs A4 is closed, the first tongs A1 and the second tongs are opened
To guarantee the normal air inlet of culture dish 3 and outlet, the third tongs A3 only opening when requiring supplementation with culture medium；It is first when sampling
The 4th tongs A4 is first opened, the second tongs and third tongs A3 are then shut off, so that due to air inlet pipe 6 in culture dish 3
Air inlet and increase, by culture solution by sample presentation pipe 9 be sent into sampling bottle 10；After obtaining enough culture solutions in sampling bottle 10, close
4th tongs A4, and the second tongs are opened, it simultaneously closes off after air pressure is normal in the first tongs A1 waiting culture dish 3 and beats again
The first tongs A1 is opened, the risk of explosion is caused to avoid 3 long-time high-pressure work of culture dish；Culture solution in sampling bottle 10 can
It connects air compressor machine by air-pressure tube 11 to be pressurized into sampling bottle 10, so that the sample in sampling bottle 10 passes through 13 row of probe tube
Out.
Culture systems in present embodiment, when adding culture medium by feed supplement pipe 8, if it is a small amount of culture medium,
Third tongs can be directly opened, culture medium is injected directly into feed supplement pipe 8 by sterilizing filter, third is then shut off and stops
Water folder, to keep gnotobasis；If culture medium is more, needs to continue addition, then culture medium is packed into feed supplement bottle, and pass through weldering
Pick and be connected to feed supplement pipe 8 feed supplement bottle by thermoplastic tube, then by sealing machine, between guarantee feed supplement bottle and thermoplastic tube and
Sealed connection between thermoplastic tube and feed supplement pipe, then third tongs are opened, so that the culture medium in feed supplement bottle passes through feed supplement pipe
Into culture dish 3.
In this way, when being sampled culture dish 3, do not need to open culture dish 3 and sink 1 in present embodiment, so that
Sampling can carry out in the culture environment of sealing, be conducive to avoid sampling the damage to culture environment；Meanwhile it is difficult to reduce sampling
Degree.
In present embodiment, temperature detection sensor 18 and heating device 20 are also equipped in sink 1.Will pass through temperature
Water temperature in 18 real-time detection sink 1 of detection sensor, and lower water temperature is carried out by heating device 20, so that culture dish 3
Always it works in suitable temperature environment.
In present embodiment, temperature detection sensor 18 is connected with the display being mounted on outside sink 1, aobvious will pass through
Show that device observes water temperature.
In present embodiment, temperature detection sensor 18 and heating device 20 are located at two sides opposite in sink 1, to guarantee
Complete detection of the temperature detection sensor 18 to water temperature in sink 1.Heating device 20 is mounted on 1 bottom of sink, to avoid heating
The heating work of device 20, which receives water level in sink 1, to be influenced.
In present embodiment, temperature detection sensor 18 is connect with heating device 20, and heating device 20 is used for according to temperature
Water temperature in the detected value adjustment sink 1 of detection sensor 18.Specifically, the side of existing intelligent control can be used in heating device
Formula, so that realizing the automatic control of water temperature in sink 1 by temperature detection sensor 18 and heating device 20.
In present embodiment, it is also equipped with water circulating pump 19 in sink 1, by water circulating pump 19 water in sink is followed
Circulation is dynamic, it is ensured that water temperature is uniformly distributed in sink.
In present embodiment, sealing-plug 21 is nested to form by the rubber ring annular of multiple and different diameters.In this way, by adjusting
Rubber ring quantity can adjust through-hole diameter, to meet the installation requirements of different pipelines.Specifically, in present embodiment, knot
Close sealing-plug 21 annular nested structure, same size can be set by each through-hole, then by sealing-plug adjustment aperture with
Distribution pipes connection requirement.In this way, simplifying the working process of sealing cover 2, and the flexibility of Pipe installing is improved, ensure that
It is seamless interspersed between pipeline and sealing cover 2, advantageously ensure that the gnotobasis inside culture systems.
In present embodiment, sealing cover 2 is hingedly mounted on sink 1, and sealing cover 2 is equipped with multiple U far from the side of shaft
Type opening 16, each U-shaped opening 16 are equipped with a spill cap liner 22, and the disassembly of spill cap liner 22 is mounted on sealing cover 2
It goes up and sealing-plug 21 is cooperated to block U-shaped opening.In this way, air inlet can be reduced by the U-shaped opening 16 that 2 edge of sealing cover is arranged in
Pipe 6, escape pipe 7, feed supplement pipe 8 and sample presentation pipe 9 pass through the difficulty of sealing cover 2.
The above, only the utility model relates to preferable specific embodiment, but the protection model of the utility model
Enclose and be not limited thereto, anyone skilled in the art within the technical scope disclosed by the utility model, according to
The technical solution of the utility model and its utility model design are subject to equivalent substitution or change, should all cover in the utility model
Within protection scope.
Claims (10)
1. a kind of culture systems characterized by comprising sink (1), sealing cover (2), culture dish (3), four-way bottle cap (5), into
Tracheae (6), escape pipe (7), feed supplement pipe (8), sample presentation pipe (9), air-pressure tube (11), probe tube (13), sampling bottle (10) and threeway
Bottle cap (14)；
Sealing cover (2) is mounted on above sink (1) for sealed cistern (1), and sealing cover (2) is equipped with multiple through-holes, and each logical
Hole is each equipped with corresponding sealing-plug (21)；Culture dish (3) is mounted in sink (1), four-way bottle cap (5) and culture dish (3)
Match and is mounted on culture dish (3) opening；Sampling bottle (10) disassembly is mounted on sink (1) and is located at sink (1) outside, threeway
Bottle cap (14) matches with sampling bottle (10) and is mounted on sampling bottle (10) opening；
Air inlet pipe (6), escape pipe (7) and feed supplement pipe (8) are installed on four-way bottle cap (5) and stretch out water through sealing cover (2)
Slot (1)；Sample presentation pipe (9) one end connects four-way bottle cap (5), and the other end connects threeway bottle cap (14)；Air inlet pipe (6), escape pipe
(7), four through-hole structures on feed supplement pipe (8) and sample presentation pipe (9) and four-way bottle cap (5) correspond；Air inlet pipe (6) and sample presentation
Pipe (9) is connected with the pipeline of insertion culture dish (3) bottom by four-way bottle cap (5) respectively；
Air-pressure tube (11) and probe tube (13) are installed on threeway bottle cap (14), sample presentation pipe (9), air-pressure tube (11) and probe tube
(13) it is corresponded with three through-hole structures on threeway bottle cap (14), probe tube (13) passes through threeway bottle cap (14) connection insertion
The pipeline of sampling bottle (10) bottom；Sterilizing filter (12) are also equipped on air-pressure tube (11)；
It is installed respectively on air inlet pipe (6), escape pipe (7), feed supplement pipe (8), sample presentation pipe (9), air-pressure tube (11) and probe tube (13)
There are the first tongs (A1), the second tongs, third tongs (A3), the 4th tongs (A4), the 5th tongs (A5) and
Six tongs (A6).
2. culture systems as described in claim 1, which is characterized in that be equipped with the first fixed frame (15) on the outside of sink (1), take
Sample bottle (10) disassembly is mounted on the first fixed frame (15).
3. culture systems as described in claim 1, which is characterized in that be equipped with the second fixed frame, culture dish inside sink (1)
(3) disassembly is mounted on the second fixed frame, and between having between culture dish (3) and sink (1) bottom and sink (1) inner wall
Gap.
4. culture systems as described in claim 1, which is characterized in that be also equipped with temperature detection sensor in sink (1)
(18) and heating device (20).
5. culture systems as claimed in claim 4, which is characterized in that temperature detection sensor (18), which is connected with, is mounted on sink
(1) external display.
6. culture systems as claimed in claim 4, which is characterized in that temperature detection sensor (18) and heating device (20) position
In two sides opposite in sink (1), heating device (20) is mounted on sink (1) bottom.
7. culture systems as claimed in claim 4, which is characterized in that temperature detection sensor (18) and heating device (20) are even
It connects, heating device (20) is used to adjust sink (1) interior water temperature according to the detected value of temperature detection sensor (18).
8. culture systems as claimed in claim 4, which is characterized in that be also equipped with water circulating pump (19) in sink (1).
9. culture systems as described in claim 1, which is characterized in that sealing-plug (21) by multiple and different diameters rubber ring
Shape is nested to form.
10. culture systems as described in any one of claim 1 to 9, which is characterized in that sealing cover (2) is hingedly mounted on sink
(1) on, sealing cover (2) is equipped with multiple U-shaped openings (16) far from the side of shaft, each U-shaped opening (16) is equipped with one
Spill cap liner (22), spill cap liner (22) disassembly are mounted on sealing cover (2) and cooperate sealing-plug (21) blocking is U-shaped to open
Mouthful.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920153341.0U CN209602548U (en) | 2019-01-29 | 2019-01-29 | A kind of culture systems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920153341.0U CN209602548U (en) | 2019-01-29 | 2019-01-29 | A kind of culture systems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209602548U true CN209602548U (en) | 2019-11-08 |
Family
ID=68402549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920153341.0U Active CN209602548U (en) | 2019-01-29 | 2019-01-29 | A kind of culture systems |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522104A (en) * | 2020-12-29 | 2021-03-19 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
-
2019
- 2019-01-29 CN CN201920153341.0U patent/CN209602548U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522104A (en) * | 2020-12-29 | 2021-03-19 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
| CN112522104B (en) * | 2020-12-29 | 2024-06-11 | 麦迪森(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
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