CN209589868U - The extraction element of high efficiency extraction tumour cell - Google Patents
The extraction element of high efficiency extraction tumour cell Download PDFInfo
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- CN209589868U CN209589868U CN201920284886.5U CN201920284886U CN209589868U CN 209589868 U CN209589868 U CN 209589868U CN 201920284886 U CN201920284886 U CN 201920284886U CN 209589868 U CN209589868 U CN 209589868U
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Abstract
The utility model discloses the extraction elements of high efficiency extraction tumour cell, the extraction element of high efficiency extraction tumour cell, including bottle body, bottle body open at one end, the lid being connected at bottle body upper end opening, through-hole is provided on lid, connecting column is provided on lid, the lower end of connecting column passes through through-hole and is located in bottle body, the baffle parallel with plane where lid is connected on connecting column, baffle is used to prevent the upper end of connecting column from passing through lid, the lower end of connecting column is connected with several for clamping the cartridge of slide, contain polymeric coating layer on slide, the polymeric coating layer contains can be with the covalently bound functional group of protein, tumor specific antibody is coated on polymeric coating layer；When rotating the upper end of connecting column, cartridge drives slide to rotate jointly；Present apparatus structure is simple, and convenient for operating with, not many and diverse step, service efficiency is higher.
Description
Technical field
The utility model relates to tumour cell fields, and in particular to the extraction element of high efficiency extraction tumour cell.
Background technique
Colorectal cancer is one of most common malignant tumor of digestive tract, and colorectal cancer has grade malignancy high, transfer morning
Feature, therefore the key for the treatment of of colorectal cancer is to early diagnose.Gut epithelium tissue is always in very fast carry out cell
It updates, at least 1% cell detachment enters enteron aisle daily, excretes with excrement, and the falling speed of tumour cell is far high
The tumour cell quantity to fall off in normal cell, excrement is far more than the circulating tumor cell in peripheral blood.By to excrement
The analysis detection of the tumour cell to fall off in sample can find cancerous information in time.And the acquisition of excrement is more more convenient than blood,
It is easier that ordinary populace is allowed to receive, compliance is more preferable.
Be common method currently with immunomagnetic beads enriched tumor cell, principle be fixed on the antibody of magnetic bead surfaces with
Tumor-cell antigen combines, and then by Magnetic Isolation, separates cast-off cells from fecal sample, obtains tumour cell
Carry out analysis detection.
But the method magnetic bead is dispersed in suspension, need to be separated by magnet, then collect magnetic bead.Not only grasp
Make cumbersome, and magnetic bead higher cost.
Generally require fast and accurately to extract when detecting, thus it is a kind of can efficiently and high speed extracts tumour cell
New device be there is an urgent need to.
Utility model content
Technical problem to be solved in the utility model is the extraction element not only inefficiency of existing tumour cell, and
And higher cost, and it is an object of the present invention to provide high efficiency extraction tumour cell extraction element, solve the problems, such as tumour cell extract.
The utility model is achieved through the following technical solutions:
The extraction element of high efficiency extraction tumour cell including bottle body, bottle body open at one end, is connected to bottle body upper end opening
The lid at place is provided with through-hole on lid, and connecting column is provided on lid, and the lower end of connecting column passes through through-hole and is located in bottle body,
The baffle parallel with plane where lid is connected on connecting column, baffle is used to prevent the upper end of connecting column from passing through lid, connection
The lower end of column is connected with several cartridges for being used to clamp slide, contains polymeric coating layer on slide, which contains energy
With the covalently bound functional group of protein, tumor specific antibody is coated on polymeric coating layer；Rotate the upper end of connecting column
When, cartridge drives slide to rotate jointly；
In use, stool suspension is placed in bottle body, the slide in cartridge is driven to adsorb in excrement by rotation connecting column
The epithelial tumor cell to fall off.
Further, when in use, stool suspension is placed in bottle body for the present apparatus, then passes through lid for the upper of bottle body
End cap is lived, and by the through hole of the lower end insertion lid of connecting column after covering, the baffle of connecting column side is located at the top of through-hole, prevents
Only the upper end of connecting column passes through through-hole, and the lower end of connecting column is connected with cartridge, and slide is stuck in cartridge, under the rotation of connecting column
The slide rotation in cartridge is driven, has polymeric coating layer on slide, polymeric coating layer contains can be with the covalently bound function of protein
Energy group, is coated with tumor specific antibody, which is covalently bound to macromolecule by functional group on polymeric coating layer
On coating, during rotation, slide stirs the intracorporal stool suspension of bottle, makes suspension during agitation
It is incubated for slide dynamic, enhances incubation effect, coated antibody passes through specific with tumor cell surface antigen on slide
The epithelial tumor cell to fall off in excrement is adsorbed in reaction.After the reaction was completed aim cell can will be directly adsorbed with by connecting column
Slide take out, direct staining microscopy, observe tumour cell, to find cancerous information.The method is not required to Magnetic Isolation, is not necessarily to
Smear operation, operates more simple and convenient.
Further, the extraction element of high efficiency extraction tumour cell, the cartridge include the company connecting with connecting column lower end
Bar, the snap-gauge connecting with connecting rod lower end side, are provided with first groove of the upper end opening lower end in snap-gauge in snap-gauge, on snap-gauge
It is additionally provided with second groove of the lower end in snap-gauge, and the lower end of the second groove is in the top of the lower end of the first groove, second
The one side of groove is connected to the side of the first groove, and the side of snap-gauge is run through in another side；
When placement, slide is stuck in the first groove, is coated with the side of tumor specific antibody close to the second groove, and
It is contacted by the second groove with the intracorporal stool suspension of bottle.
Cartridge is set as above structure, can directly be put into slide in snap-gauge above the first groove when in use, and
Be fixed in snap-gauge by the first groove, then by the second groove make slide be coated with antibody side and the intracorporal excrement of bottle
Suspension directly contacts, and effectively slide can not only be fixed in this way, and when pick-and-place can directly pick and place, and revolves in connecting rod
It during turning, during being placed on rotation as slide, will not be thrown out of, be more convenient for using while slide and excrement
The contact area of suspension is bigger, further improves extraction efficiency.
Preferably, the extraction element of high efficiency extraction tumour cell, there are four groove, groove and companies for the lower end setting of connecting column
The upper end of bar is connected by screw thread rotation.Setting is there are four groove, so when in use, can be arranged simultaneously multiple slides simultaneously into
Row extracts, and increases response area, can further improve efficiency.
Preferably, the extraction element of high efficiency extraction tumour cell, connecting rod are located at snap-gauge institute planar.Such setting exists
When rotation, slide can be more advantageous to the agitation of stool suspension during rotation, make it more along straight line rotation where connecting rod
It is good with antibody ining conjunction with, absorption aim cell.
Preferably, the extraction element of high efficiency extraction tumour cell, the height of slide are higher than the height of the first groove.Slide
Height is higher than the height of the first groove, therefore when slide to be placed in the first groove, the upper end of slide is in the first groove
Upper end can directly take out when taking out, and it is more convenient to take out.
Specifically, the extraction element of high efficiency extraction tumour cell, connecting rod are vertically arranged, plane is in perpendicular where snap-gauge
It is interior.Connecting rod is placed vertically, and during connecting rod rotation, the side of snap-gauge is using straight line where connecting rod as the cylinder of axis.
Further, the extraction element of high efficiency extraction tumour cell, the tumor specific antibody are Epithelial Cell Adhesion
The specific antibody of molecule EpCAM.
Epithelial cell adhesion molecule EpCAM is a kind of epithelial cell specific antigen, belongs to a kind of transmembrane glycoprotein, is participated in
Adjust cell adhesion, proliferation, differentiation, migration and signal transduction.EpCAM in normal epithelium cell except expressing, also many swollen
Unconventionality expression in oncocyte.EpCAM in colorectal cancer most early in having found.EpCAM high is expressed in colorectal cancer cell.EpCAM is
A kind of important tumor marker.
Preferably, the extraction element of high efficiency extraction tumour cell, lid are connect with bottle body by screw thread rotation.Pass through screw thread
Rotation connection, it is more convenient in connection, and when removing, also it is more convenient.
The utility model compared with prior art, has the following advantages and benefits:
1, the extraction element of the utility model high efficiency extraction tumour cell, this device of the present apparatus are not required to immunomagnetic beads, are not required to
Magnetic Isolation, it is easy to operate flexible, it is low in cost；
2, the extraction element of the utility model high efficiency extraction tumour cell, the present apparatus is using on tumor-cell antigen and slide
The specific binding of antibody can capture the neoplastic epithelial cells to fall off in excrement, can quickly detect to colorectal cancer；
3, the extraction element of the utility model high efficiency extraction tumour cell, present apparatus structure is simple, convenient for operating with, does not have
There is many and diverse step, service efficiency is higher.
Detailed description of the invention
Attached drawing described herein is used to provide to further understand the utility model embodiment, constitutes the one of the application
Part does not constitute the restriction to the utility model embodiment.In the accompanying drawings:
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is the utility model Self-locking sealing antitheft box schematic diagram；
Fig. 3 is the utility model snap-gauge top view；
Fig. 4 is the utility model connecting column lower end structure schematic diagram.
Label and corresponding parts title in attached drawing:
1- bottle body, 2- lid, 3- baffle, 4- connecting column, 5- connecting rod, 6- snap-gauge, the first groove of 7-, the second groove of 8-, 9-
Slide, 10- groove.
Specific embodiment
For the purpose of this utility model, technical solution and advantage is more clearly understood, below with reference to embodiment and attached drawing,
The utility model is described in further detail, and the exemplary embodiment and its explanation of the utility model are only used for explaining this
Utility model is not intended to limit the scope of the present invention.
Embodiment 1
As shown in figures 1-4, the extraction element of the utility model high efficiency extraction tumour cell, including it is bottle body 1, open at one end
Bottle body 1, the lid 2 that is connected at 1 upper end opening of bottle body, be provided with through-hole on lid 2, connecting column 4 be provided on lid 2,
The lower end of connecting column 4 passes through through-hole and is located in bottle body 1, and the baffle 3 parallel with 2 place plane of lid is connected on connecting column 4, keeps off
Plate 3 is used to prevent the upper end of connecting column 4 from passing through lid 2, and the lower end of connecting column 4 is connected with several cartridges for being used to clamp slide 9,
Contain polymeric coating layer on slide 9, which contains can be with the covalently bound functional group of protein, polymeric coating layer
On be coated with tumor specific antibody；When rotating the upper end of connecting column 4, cartridge drives slide 9 to rotate jointly；
In use, stool suspension is placed in bottle body 1, the slide 9 in cartridge is driven to adsorb excrement by rotation connecting column 4
In the epithelial tumor cell that falls off.
Embodiment 2
The extraction element of high efficiency extraction tumour cell, on the basis of embodiment 1, the cartridge include under connecting column 4
The connecting rod 5 connected, the snap-gauge 6 connecting with 5 lower end side of connecting rod are held, is provided with upper end opening lower end in snap-gauge 6 in snap-gauge 6
First groove 7 is additionally provided with second groove 8 of the lower end in snap-gauge 6 on snap-gauge 6, and the lower end of the second groove 8 is recessed first
The top of the lower end of slot 7, the one side of the second groove 8 are connected to the side of the first groove 7, and the side of snap-gauge 6 is run through in another side
Face；
When placement, slide 9 is stuck in the first groove 7, is coated with the side of tumor specific antibody close to the second groove 8,
And it is contacted by the second groove 8 with the stool suspension in bottle body 1.
Embodiment 3
The extraction element of high efficiency extraction tumour cell, on the basis of embodiment 2, there are four the lower end settings of connecting column 4
Groove 10, groove 10 are connect with the upper end of connecting rod 5 by screw thread rotation.Connecting rod 5 is located at 6 institute of snap-gauge planar.The height of slide 9
Degree is higher than the height of the first groove 7.Connecting rod 5 is vertically arranged, and 6 place plane of snap-gauge is in perpendicular.The tumour-specific
Antibody is the specific antibody of epithelial cell adhesion molecule EpCAM.Lid 2 is connect with bottle body 1 by screw thread rotation.
In use, acquisition fresh excreta, fecal sample is diluted, then by strainer excrement is filtered to remove biggish insoluble
Property particle and fiber, obtain cell suspending liquid.Lid 2 is opened, stool suspension is injected in bottle body 1, first in snap-gauge 6 is recessed
Slide is inserted into slot 7, the upper end of connecting rod 5 passes through the through-hole of lid, is threadedly coupled with the groove of 4 lower end of connecting column, after connection, spiral shell
Line rotation closes the lid 2, and the upper end of hand-held connecting column 4 is rotated, and when rotation drives slide rotation, the connecting column 4 of the present apparatus
Upper end slide can be made to rotate automatically with connecting power device, power device, be incubated for.
Directly the slide for being adsorbed with cell can be taken out after the reaction was completed, the liquid in bottle is removed, inject cleaning solution, lid
Upper cover, rotation slide is washed, then slide is taken out, and repeats this step.Finally lid 2 is rotated and is taken out, then by slide
It is taken out out of second groove.By Coplin microscopy, tumour cell and quantity have been seen whether, judge the pernicious of colorectal cancer
Degree.The polymeric coating layer that the present apparatus uses can be polystyrene, polyvinyl chloride, polyethyleneimine.
Above-described specific embodiment, to the purpose of this utility model, technical scheme and beneficial effects carried out into
One step is described in detail, it should be understood that being not used to limit the foregoing is merely specific embodiment of the present utility model
Determine the protection scope of the utility model, within the spirit and principle of the utility model, any modification for being made equally is replaced
It changes, improve, should be included within the scope of protection of this utility model.
Claims (8)
1. the extraction element of high efficiency extraction tumour cell, which is characterized in that including bottle body (1), bottle body open at one end (1), connect
The lid (2) at bottle body (1) upper end opening is connect, through-hole is provided on lid (2), connecting column (4) are provided on lid (2),
The lower end of connecting column (4) passes through through-hole and is located in bottle body (1), is connected on connecting column (4) parallel with plane where lid (2)
Baffle (3), baffle (3) are used to prevent the upper end of connecting column (4) from passing through lid (2), and the lower end of connecting column (4) is connected with several use
In the cartridge of clamping slide (9), slide contains polymeric coating layer on (9), which, which contains, covalently to tie with protein
The functional group of conjunction is coated with tumor specific antibody on polymeric coating layer；When rotating the upper end of connecting column (4), cartridge is driven
Slide (9) rotates jointly；
In use, stool suspension is placed in bottle body (1), the slide (9) in cartridge is driven to adsorb excrement by rotation connecting column (4)
Just the epithelial tumor cell to fall off in.
2. the extraction element of high efficiency extraction tumour cell according to claim 1, which is characterized in that the cartridge include with
The connecting rod (5) of connecting column (4) lower end connection, the snap-gauge (6) connecting with connecting rod (5) lower end side, snap-gauge (6) is interior to be provided with upper end
First groove (7) of the open lower end in snap-gauge (6) is additionally provided with second groove of the lower end in snap-gauge (6) on snap-gauge (6)
(8), and the lower end of the second groove (8) is in the top of the lower end of the first groove (7), the one side and first of the second groove (8)
The side of groove (7) is connected to, and the side of snap-gauge (6) is run through in another side；
When placement, slide (9) is stuck in the first groove (7), is coated with the side of tumor specific antibody close to the second groove
(8), it and by the second groove (8) is contacted with the stool suspension in bottle body (1).
3. the extraction element of high efficiency extraction tumour cell according to claim 2, which is characterized in that under connecting column (4)
There are four groove (10), groove (10) is connect with the upper end of connecting rod (5) by screw thread rotation for end setting.
4. the extraction element of high efficiency extraction tumour cell according to claim 2, which is characterized in that connecting rod (5) is located at card
Plate (6) institute is planar.
5. the extraction element of high efficiency extraction tumour cell according to claim 2, which is characterized in that the height of slide (9)
Higher than the height of the first groove (7).
6. the extraction element of high efficiency extraction tumour cell according to claim 4, which is characterized in that connecting rod (5) is set vertically
It sets, plane where snap-gauge (6) is in perpendicular.
7. the extraction element of high efficiency extraction tumour cell according to claim 1, which is characterized in that the tumour-specific
Antibody is the specific antibody of epithelial cell adhesion molecule EpCAM.
8. the extraction element of high efficiency extraction tumour cell according to claim 1, which is characterized in that lid (2) and bottle body
(1) it is connected by screw thread rotation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920284886.5U CN209589868U (en) | 2019-03-06 | 2019-03-06 | The extraction element of high efficiency extraction tumour cell |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920284886.5U CN209589868U (en) | 2019-03-06 | 2019-03-06 | The extraction element of high efficiency extraction tumour cell |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111714930A (en) * | 2020-05-15 | 2020-09-29 | 浙江省肿瘤医院 | Separation medium and method for enriching and separating circulating tumor cells from blood sample |
| CN112972783A (en) * | 2019-12-13 | 2021-06-18 | 周菁 | Malignant chest abdomen aquatic separation purification tumor cell device |
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2019
- 2019-03-06 CN CN201920284886.5U patent/CN209589868U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112972783A (en) * | 2019-12-13 | 2021-06-18 | 周菁 | Malignant chest abdomen aquatic separation purification tumor cell device |
| CN112972783B (en) * | 2019-12-13 | 2024-04-05 | 陕西省人民医院 | Device for separating and purifying tumor cells in malignant hydrothorax and ascites |
| CN111714930A (en) * | 2020-05-15 | 2020-09-29 | 浙江省肿瘤医院 | Separation medium and method for enriching and separating circulating tumor cells from blood sample |
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