CN209559763U - A kind of serium vitamin A detection kit - Google Patents
A kind of serium vitamin A detection kit Download PDFInfo
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- CN209559763U CN209559763U CN201920072732.XU CN201920072732U CN209559763U CN 209559763 U CN209559763 U CN 209559763U CN 201920072732 U CN201920072732 U CN 201920072732U CN 209559763 U CN209559763 U CN 209559763U
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- drawer
- plate
- vitamin
- detection
- storage chamber
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Abstract
This reality is novel to disclose a kind of serium vitamin A detection kit, including hard frame body, packing carton and detection plate, the hard frame body is rectangular frame structure, and the inside of hard frame body passes through the shelf being uniformly arranged and is equipped with the first storage chamber, second storage chamber and third storage chamber, the outside of hard frame body is uniformly enclosed with packing carton simultaneously, the internal activity of the third storage chamber is equipped with the first drawer, the two sides of second storage chamber are respectively arranged with the second drawer and third drawer, and the second drawer is internally provided with agent plate, third drawer is internally provided with storage shelf, first storage chamber is internally provided with detection plate, the top of the packing carton side is equipped with the upper cover plate folded inwardly.The utility model carries out vitamin A content detection using the support plate that 96 hole micropores are constituted, and realizes quick detection, detection efficiency is much higher than traditional detection method.
Description
Technical field
The utility model relates to kit technical field, specially a kind of serium vitamin A detection kit.
Background technique
It is well known that existing VA detection method has fluorescence method, high performance liquid chromatography and electrochemical process at present.These sides
Method is generally needed using detection kit, and in order to meet the needs of detection, detection kit largely occurs, it is well known, however, that examination
The generally existing following disadvantage of agent box:
(1) interior of detection kit is more unreasonable, and reagent and instrument are generally arranged at same layer, so that reagent
Put with instrument confusing, testing staff can not rapidly take reagent and instrument at work, to increase detection people
The workload of member, reduces detection speed；
(2) detection kit quality is softer, is easy to appear extruding during transportation, causes reagent and instrument that position occurs
Offset, or even reagent instrument damage is caused because mutually colliding；
(3) in testing staff's detection process detection plate put with reagent it is unreasonable so that detection efficiency greatly receives
It influences, reduces detection efficiency.
Utility model content
The purpose of this utility model is to provide a kind of serium vitamin A detection kits, to solve above-mentioned background technique
The problem of middle proposition.
To achieve the above object, the utility model provides the following technical solutions: a kind of serium vitamin A detection kit,
Including hard frame body, packing carton and detection plate, the hard frame body is rectangular frame structure, and the inside of hard frame body is logical
It crosses the shelf being uniformly arranged and is equipped with the first storage chamber, the second storage chamber and third storage chamber, while the outside of hard frame body is equal
Even to be enclosed with packing carton, the internal activity of the third storage chamber is equipped with the first drawer, the two sides difference of the second storage chamber
It is provided with the second drawer and third drawer, the centre of the first drawer, the second drawer and third drawer one end is all equipped with first and leads
Leader tape, and the second drawer is internally provided with agent plate, and third drawer is internally provided with storage shelf, while on storage shelf
Even to be provided with fixation hole, the centre at the top of the agent plate is equipped with groove, and the inside of groove is filled with foam, and foam is interior
Portion is uniformly provided with the first reagent wells and the second reagent wells, while the both ends at the top of agent plate are all fixed with upper frame, and described first
Storage chamber is internally provided with detection plate, and the both ends of detection plate bottom are all provided with the card slot being mutually matched with upper frame, and block
Detection plate bottom on the inside of slot is fixed with support frame, while the centre of detection plate is provided with support plate, and the top of support plate is uniform
It is provided with micropore, the top of the packing carton side is equipped with the upper cover plate folded inwardly, is arranged on the inner sidewall of upper cover plate
Flexible band, and the junction of elastic webbing and upper cover plate is evenly arranged with folder hole.
Further, the bottom of the packing carton side offers the first opening being mutually matched with the first drawer, and
And the centre at packing carton both ends all offers the second opening being mutually matched with the second drawer and third drawer and third is open.
Further, the centre at both ends is all equipped with the second leash at the top of the detection plate.
Further, the micropore quantity at the top of the support plate is 96 holes.
Further, the inside of first drawer is evenly arranged with matter storage lattice by cross partition.
Further, the examination that vitamin A standard solution, enhanced sensitivity reagent, deproteinizing agent are housed is placed in second drawer
Agent bottle.
Further, the concentration of standard solution of the vitamin A is 10 μ g/dl, 20 μ g/dl and 30 μ g/dl.
Further, the enhanced sensitivity reagent is the micellar solution being made of surfactant and cosurfactant or micro emulsion
Liquid.
Further, the surfactant is anionic surfactant, cationic surfactant or nonionic table
Face activating agent.
Further, the cosurfactant is ethyl alcohol or ethylene glycol.
Compared with prior art, the utility model has the beneficial effects that
(1) the utility model cooperates the outside of hard frame body uniform by the hard frame body in setting rectangular frame structure
The packing carton of package constitutes complete reagent cartridge configuration, can greatly enhance the structural strength of kit, can effectively keep away
Exempt to be easy to appear extruding during transportation；
(2) the utility model is uniformly arranged the first storage chamber, the second storage by the shelf of the inner utilization in hard frame body
Object chamber and third storage chamber, and the first drawer, the two sides of the second storage chamber are installed in the internal activity of third storage chamber
It is respectively arranged with the second drawer and third drawer, instrument and reagent can be effectively individually positioned in the drawer of different storage chambers
In, the placement position of instrument and reagent has effectively been distinguished, has understood its pattern, well arranged, articles ride is orderly；
(3) the utility model is combined with each other the detection plate and agent plate that use by being arranged, can in the detection process by
Detection plate is placed in the top of agent plate, convenient for the use of testing staff, is conducive to the raising for detecting speed；
(5) the utility model carries out vitamin A content detection using the support plate that 96 hole micropores are constituted, and realizes quick inspection
It surveys, detection efficiency is much higher than traditional detection method.
Detailed description of the invention
Fig. 1 is the overall structure diagram of the utility model；
Fig. 2 is the hard frame structure schematic diagram of the utility model；
Fig. 3 is that structural schematic diagram is applied in combination in the detection plate of the utility model and agent plate.
In figure: the first drawer of 1-；2- agent plate；The second drawer of 3-；4- micropore；5- detection plate；6- elastic webbing；7- upper cover
Plate；8- folder hole；9- packing carton；10- third drawer；11- storage shelf；12- fixation hole；The first leash of 13-；14- second is led
Leader tape；15- upper frame；16- support plate；17- support frame；18- card slot；19- groove；The first reagent wells of 20-；21- foam；22-
Two reagent wells；The first storage chamber of 23-；24- third storage chamber；25- hard frame body；The second storage chamber of 26-；27- shelf.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1-3 is please referred to, a kind of embodiment provided by the utility model: a kind of serium vitamin A detection kit, including
Hard frame body 25, packing carton 9 and detection plate 5, hard frame body 25 is rectangular frame structure, and the inside of hard frame body 25 is logical
It crosses the shelf 27 being uniformly arranged and is equipped with the first storage chamber 23, the second storage chamber 26 and third storage chamber 24, while hard frame body
25 outside is uniformly enclosed with packing carton 9, and the internal activity of third storage chamber 24 is equipped with the first drawer 1, the first drawer 1
Inside is evenly arranged with matter storage lattice, matter storage lattice internal storage rubber head dropper, test tube and other items, the second storage chamber by cross partition
26 two sides are respectively arranged with the second drawer 3 and third drawer 10, and the bottom of 9 side of packing carton offers and the first drawer 1
The first opening being mutually matched, and the centre at 9 both ends of packing carton all offer it is mutual with the second drawer 3 and third drawer 10
Matched second opening and third opening, the centre of 10 one end of the first drawer 1, the second drawer 3 and third drawer are all equipped with the
One leash 13, and the second drawer 3 is internally provided with agent plate 2, and third drawer 10 is internally provided with storage shelf 11, together
When storage shelf 11 on be evenly arranged with fixation hole 12, fixation hole 12 is equipped with groove for fixing medicament, the centre at 2 top of agent plate
19, the inside of groove 19 is filled with foam 21, and foam 21 has good shock-absorbing function, avoids reagent bottle from damaging, and foam
21 inside is uniformly provided with the first reagent wells 20 and the second reagent wells 22, will equipped with vitamin A standard solution, enhanced sensitivity reagent,
The reagent bottle of deproteinizing agent is placed in the first reagent wells 20 or the second reagent wells 22, while the both ends at 2 top of agent plate are all fixed
There is upper frame 15, the first storage chamber 23 is internally provided with detection plate 5, and the centre at 5 top both ends of detection plate is all equipped with second and leads
Leader tape 14, the setting of the second leash 14 conveniently will test plate 5 and take out inside the first storage chamber 23, and the two of 5 bottom of detection plate
End is all provided with the card slot 18 being mutually matched with upper frame 15, and 5 bottom of detection plate of 18 inside of card slot is fixed with support frame 17,
In use process, the top that plate 5 is placed in agent plate 2 will test, the card slot 18 of 5 bottom of detection plate is arranged the top with upper frame 15 at this time
Portion cooperates support frame 17, plays the effect of dual support, guarantees the stability that detection plate 5 and agent plate 2 are applied in combination, simultaneously
The centre of detection plate 5 is provided with support plate 16, detection plate 5 and 16 integral structure of support plate, and the top of support plate 16 is uniformly arranged
There is micropore 4,4 quantity of micropore at 16 top of support plate is 96 holes, and the top of 9 side of packing carton is equipped with the upper cover folded inwardly
Plate 7 is provided with elastic webbing 6 on the inner sidewall of upper cover plate 7, and the junction of elastic webbing 6 and upper cover plate 7 is evenly arranged with folder hole
8, there is elastic webbing 6 certain elastic space and folder hole 8 to cooperate, for gripping test tube.
Working principle: the instruments such as rubber head dropper, test tube are placed in the matter storage lattice in the first drawer 1, are 10 μ equipped with concentration
G/dl, the vitamin A standard solution of 20 μ g/dl and 30 μ g/dl, enhanced sensitivity reagent, deproteinizing agent reagent bottle be placed in the second drawer 3
In the first reagent wells 20 and the second reagent wells 22 in, in use process, the agent plate 2 inside the second drawer 3 can be taken out, together
The second leash of Shi Liyong 14 will test plate 5 and take out from the inside of the first storage chamber 23, and will test plate 5 and be placed in agent plate 2
Top, the card slot 18 of 5 bottom of detection plate is arranged the top with upper frame 15 at this time, to realize the combination of detection plate 5 Yu agent plate 2
It uses, convenient for the reagent of taking of testing staff, is conducive to the raising for detecting speed.
Detecting step:
Step 1: to take 20 μ l concentration respectively be 10 μ g/dl, 20 μ g/dl, the vitamin A standard solution of 30 μ g/dl and to be measured
Blood serum sample, is denoted as standard group A, standard group B, standard group C and experimental group respectively, and above-mentioned each group is 3 parallel laboratory tests, Xiang Biao
20 μ l deproteinizing agents are respectively added in quasi- group A, standard group B, standard group C and experimental group, 3000r/min is centrifuged 15min, takes supernatant
Liquid；
Step 2: the supernatant in step 1 is added sequentially in 96 hole microwell plates, and is separately added into the examination of 180 μ l enhanced sensitivities
Agent is incubated for 3h in 60 DEG C of water-baths；
Step 3: it after incubation, is measured using microwell plate fluorescence analyser with excitation wavelength 345nm, launch wavelength
480nm measures fluorescent value, with the concentration of each gradient of the standard solution of vitamin A and fluorescent value fit standard curve, by blood to be measured
The fluorescent value of final proof product substitutes into standard curve the content for calculating vitamin A.
Embodiment 1:
Step 1: the standard solution (10 μ g/dl, 20 μ g/dl and 30 μ g/dl) and blood to be measured of 20 μ l vitamin As are taken respectively
Final proof product, are respectively added deproteinizing agent, carry out removing protein processing, and the volume ratio of test serum sample and deproteinizing agent is 1:1, then
3000r/min is centrifuged 15min, takes supernatant.
Step 2: above-mentioned supernatant is added sequentially in 96 hole microwell plates, and is separately added into 180 μ l enhanced sensitivity reagents (yin
Ionic surface active agent and alcohol mixture), 3h is incubated in 60 DEG C of water-baths.
Step 3: it is detected using microwell plate fluorescence analyser, with excitation wavelength 345nm, it is glimmering that launch wavelength 480nm measures its
Light value, with the concentration of each gradient of the standard solution of vitamin A and fluorescent value fit standard curve, by the fluorescence of test serum sample
Value substitutes into standard curve the content for calculating vitamin A.
Embodiment 2:
Step 1: the standard solution (10 μ g/dl, 20 μ g/dl and 30 μ g/dl) and blood to be measured of 20 μ l vitamin As are taken respectively
Final proof product, are respectively added deproteinizing agent, carry out removing protein processing, and the volume ratio of test serum sample and deproteinizing agent is 1:1, then
3000r/min is centrifuged 15min, takes supernatant.
Step 2: above-mentioned supernatant is added sequentially in 96 hole microwell plates, and is separately added into 180 μ l enhanced sensitivity reagents (sun
Ionic surface active agent and alcohol mixture), 3h is incubated in 60 DEG C of water-baths.
Step 3: it is detected using microwell plate fluorescence analyser, with excitation wavelength 345nm, it is glimmering that launch wavelength 480nm measures its
Light value, with the concentration of each gradient of the standard solution of vitamin A and fluorescent value fit standard curve, by the fluorescence of test serum sample
Value substitutes into standard curve the content for calculating vitamin A.
Embodiment 3:
Step 1: the standard solution (10 μ g/dl, 20 μ g/dl and 30 μ g/dl) and blood to be measured of 20 μ l vitamin As are taken respectively
Final proof product, are respectively added deproteinizing agent, carry out removing protein processing, and the volume ratio of test serum sample and deproteinizing agent is 1:1, then
3000r/min is centrifuged 15min, takes supernatant.
Step 2: above-mentioned supernatant is added sequentially in 96 hole microwell plates, and it is (non-to be separately added into 180 μ l enhanced sensitivity reagents
Surfactant and ethylene glycol mixture), 3h is incubated in 60 DEG C of water-baths.
Step 3: it is detected using microwell plate fluorescence analyser, with excitation wavelength 345nm, it is glimmering that launch wavelength 480nm measures its
Light value, with the concentration of each gradient of the standard solution of vitamin A and fluorescent value fit standard curve, by the fluorescence of test serum sample
Value substitutes into standard curve the content for calculating vitamin A.
Embodiment 4:
Step 1: the standard solution (10 μ g/dl, 20 μ g/dl and 30 μ g/dl) and blood to be measured of 20 μ l vitamin As are taken respectively
Final proof product, are respectively added deproteinizing agent, carry out removing protein processing, and the volume ratio of test serum sample and deproteinizing agent is 1:1, then
3000r/min is centrifuged 15min, takes supernatant.
Step 2: above-mentioned supernatant is added sequentially in 96 hole microwell plates, and is separately added into 180 μ l enhanced sensitivity reagents (yin
Ionic surface active agent and ethylene glycol mixture), 3h is incubated in 60 DEG C of water-baths.
Step 3: it is detected using microwell plate fluorescence analyser, with excitation wavelength 345nm, it is glimmering that launch wavelength 480nm measures its
Light value, with the concentration of each gradient of the standard solution of vitamin A and fluorescent value fit standard curve, by the fluorescence of test serum sample
Value substitutes into standard curve the content for calculating vitamin A.
In conclusion doing 10 repeatability analyses, interassay coefficient of variation 4.97%, same serum with same serum specimen
Sample parallel analysis 10 times, serum variation within batch coefficient is 2.87%.
For vitamin A concentration in 1-50 μ g/dl, standard curve is linearly good.
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and
And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this is practical new in other specific forms
Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this is practical new
The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing for the equivalent requirements of the claims will be fallen in
All changes in justice and range are embraced therein.It should not treat any reference in the claims as limiting
Related claim.
Claims (10)
1. a kind of serium vitamin A detection kit, which is characterized in that including hard frame body (25), the hard frame body (25)
For rectangular frame structure, and the inside of the hard frame body (25) passes through the shelf (27) being uniformly arranged and is equipped with the first storage chamber
(23), the outside of the second storage chamber (26) and third storage chamber (24), the hard frame body (25) is uniformly enclosed with packing carton
(9), the internal activity of the third storage chamber (24) is equipped with the first drawer (1), the two sides point of second storage chamber (26)
It is not provided with the second drawer (3) and third drawer (10), first drawer (1), the second drawer (3) and third drawer (10) one
The centre at end is all equipped with the first leash (13), and second drawer (3) is internally provided with agent plate (2), and described
Three drawers (10) are internally provided with storage shelf (11), while fixation hole (12), institute are evenly arranged on the storage shelf (11)
It states the centre at the top of agent plate (2) and is equipped with groove (19), the inside of the groove (19) is filled with foam (21), the foam
(21) inside is uniformly provided with the first reagent wells (20) and the second reagent wells (22), and the both ends at the top of the agent plate (2) are all
It being fixed with upper frame (15), first storage chamber (23) is internally provided with detection plate (5), and the two of detection plate (5) bottom
End is all provided with the card slot (18) being mutually matched with upper frame (15), and detection plate (5) bottom on the inside of the card slot (18) is fixed to be connected
It is connected to support frame (17), the centre of the detection plate (5) is provided with support plate (16), and the top of the support plate (16) is uniformly set
It is equipped with micropore (4), the top of packing carton (9) side is equipped with the upper cover plate (7) folded inwardly, the upper cover plate (7)
Inner sidewall on be provided with elastic webbing (6), the junction of the elastic webbing (6) and upper cover plate (7) is evenly arranged with folder hole (8).
2. a kind of serium vitamin A detection kit according to claim 1, which is characterized in that the packing carton (9)
The bottom of side offers the first opening being mutually matched with the first drawer (1), and the centre at packing carton (9) both ends is all opened
It is open equipped with the second opening being mutually matched with the second drawer (3) and third drawer (10) and third.
3. a kind of serium vitamin A detection kit according to claim 1, which is characterized in that detection plate (5) top
The centre at portion both ends is all equipped with the second leash (14).
4. a kind of serium vitamin A detection kit according to claim 1, which is characterized in that the support plate (16)
Micropore (4) quantity at top is 96 holes.
5. a kind of serium vitamin A detection kit according to claim 1, which is characterized in that first drawer (1)
Inside matter storage lattice is evenly arranged with by cross partition.
6. a kind of serium vitamin A detection kit according to claim 1, which is characterized in that second drawer (3)
It is middle to place the reagent bottle that vitamin A standard solution, enhanced sensitivity reagent, deproteinizing agent are housed.
7. a kind of serium vitamin A detection kit according to claim 6, which is characterized in that the mark of the vitamin A
Quasi- solution concentration is 10 μ g/dl, 20 μ g/dl and 30 μ g/dl.
8. a kind of serium vitamin A detection kit according to claim 6, which is characterized in that the enhanced sensitivity reagent is
The micellar solution or microemulsion be made of surfactant and cosurfactant.
9. a kind of serium vitamin A detection kit according to claim 8, which is characterized in that the surfactant
For anionic surfactant, cationic surfactant or nonionic surfactant.
10. a kind of serium vitamin A detection kit according to claim 8, which is characterized in that described to help surface-active
Agent is ethyl alcohol or ethylene glycol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920072732.XU CN209559763U (en) | 2019-01-16 | 2019-01-16 | A kind of serium vitamin A detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920072732.XU CN209559763U (en) | 2019-01-16 | 2019-01-16 | A kind of serium vitamin A detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209559763U true CN209559763U (en) | 2019-10-29 |
Family
ID=68307474
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920072732.XU Expired - Fee Related CN209559763U (en) | 2019-01-16 | 2019-01-16 | A kind of serium vitamin A detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209559763U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109459421A (en) * | 2019-01-16 | 2019-03-12 | 岳昆 | A kind of serium vitamin A detection kit and its detection method |
-
2019
- 2019-01-16 CN CN201920072732.XU patent/CN209559763U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109459421A (en) * | 2019-01-16 | 2019-03-12 | 岳昆 | A kind of serium vitamin A detection kit and its detection method |
| CN109459421B (en) * | 2019-01-16 | 2024-03-08 | 岳昆 | Serum vitamin A detection kit and detection method thereof |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191029 Termination date: 20200116 |
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| CF01 | Termination of patent right due to non-payment of annual fee |