CN209559739U - A kind of elisa assay instrument - Google Patents
A kind of elisa assay instrument Download PDFInfo
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- CN209559739U CN209559739U CN201822252466.7U CN201822252466U CN209559739U CN 209559739 U CN209559739 U CN 209559739U CN 201822252466 U CN201822252466 U CN 201822252466U CN 209559739 U CN209559739 U CN 209559739U
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- axis component
- loophole
- elisa assay
- assay instrument
- testing agency
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Abstract
The utility model discloses a kind of elisa assay instrument, belong to automatic detection system technical field.The elisa assay instrument of the utility model is equipped with X-axis component on the rack, is fixedly mounted on X-axis component Z axis component, reagent placement mechanism, wiper mechanism and the chip fixed mechanism arranged in X direction and testing agency；X-axis Component driver Z axis component is mobile in X-direction；Injection mechanism is installed, it is mobile in Z-direction for Z axis Component driver on Z axis component；Reagent placement mechanism, wiper mechanism and chip fixed mechanism are below injection mechanism；Testing agency includes laser emitter, optical signal detector and the first loophole, filter and the second loophole between laser emitter and optical signal detector, and the first loophole and the second loophole are coaxially disposed.The utility model realizes full-automatic detection, and testing agency can utilize the straightline propagation and black material extinction principle of light, and effective exclusive PCR light improves detection sensitivity and detection accuracy.
Description
Technical field
The utility model relates to a kind of elisa assay instrument, belong to automatic detection system technical field.
Background technique
ELISA is a kind of immunoassays (immunoassay, IA), basis be antigen or antibody immobilization and antigen or
The enzyme of antibody marks.After the substrate of enzyme reaction is added, substrate becomes color products by enzymatic, is examined in the amount and sample of product
The amount of substance is directly related, thus carries out qualitative or quantitative analysis.Due to various antigen compositions, the haptens including small molecule,
The antiserum or monoclonal antibody that can be used to prepare specificity just can detect corresponding in sample using this antibody as reagent
Antigen, therefore the application range of immunoassays is extremely wide.
The core of elisa assay instrument is all a colorimeter, and the content of antigen or antibody is analyzed with colorimetric method, further includes
The operating procedures such as sample-adding, heat preservation, washing, colour developing.ELSIA operating procedure is complicated, influences that reaction factor is more, and especially solid phase carries
The coating hardly possible of body reaches consistent between each individual, therefore in quantitative determination, every batch of test must use a series of various concentrations
Reference standard product make standard curve at identical conditions.Existing elisa assay instrument is substantially that the enzyme-linked of separate type is exempted from
Epidemic disease instrument, each step is operated respectively, cumbersome, is easy error, and error influence factor is larger.In addition, existing
Elisa assay instrument optical detection system in, it is ineffective to the exclusion of interference light, be affected to detection sensitivity, or
Person is to need to operate using magazine, cumbersome, and to the more demanding of equipment.
Utility model content
In order to solve the above technical problems, the utility model provides a kind of elisa assay instrument, the ELISA of the utility model divides
Analyzer is the automated analysis detection device of high integration, has automatic sampling sample-adding, cleans automatically, is temperature automatically controlled, automatic inspection
The functions such as survey, and the optical path testing agency of the utility model design, can utilize straightline propagation and the black material extinction of light
Principle, effective exclusive PCR light improves detection sensitivity and detection accuracy.
First purpose of the utility model is to provide a kind of elisa assay instrument, including rack；
Be equipped in the rack X-axis component, Z axis component, reagent placement mechanism, wiper mechanism, chip fixed mechanism and
Testing agency；
The Z axis component is fixedly mounted on X-axis component, can be mobile in X-direction by X-axis Component driver；The Z axis
Injection mechanism is installed, the injection mechanism can be by the lower movement in z-direction of Z axis Component driver on component；In conjunction with Z axis component
Can be mobile in X-direction by X-axis Component driver, injection mechanism can be moved in X-direction and Z-direction；
Reagent placement mechanism, wiper mechanism and the chip fixed mechanism is mounted on the lower section of injection mechanism, and tries
Agent placement mechanism, wiper mechanism and chip fixed mechanism are arranged in X direction；Injection mechanism can be in reagent placement mechanism and chip
It is moved between fixed mechanism, the operation such as is loaded, is sampled, cleaned；
The testing agency includes laser emitter, optical signal detector and is successively set on laser emitter and light and believes
The first loophole, filter and the second loophole between number detector, first loophole and the second loophole are coaxial
Setting, the testing agency are prepared using black material.
Further, when detection, chip is placed between laser emitter and the first loophole.
Further, the filter is the filter through laser transmitter projects wavelength.
Further, the testing agency further includes laser emitter limiting device, the laser emitter limit
Device includes third loophole and laser emitter limit hole, and the third loophole and the first loophole are coaxially disposed.Swash
The light of optical transmitting set transmitting is projected by third loophole, through chip to be detected, then in turn through the first loophole, is filtered
Piece and second penetrates hole, reaches optical signal detector and is detected.
Further, the reagent placement mechanism includes the first temperature control equipment, orifice plate and setting in reagent orifice plate
On lid；The lid can be mobile in X-direction.Reagent orifice plate can be 96 orifice plates, and the first temperature control equipment can be real
Existing 2 DEG C~25 DEG C of accurate control, realizes the protection to reagent.
Further, the wiper mechanism includes rinse bath and spray equipment.Cleaning solution is pumped into spray equipment, is led to
The mode of partial high pressure spray cleaning is crossed to cleaning outside injection mechanism, while injection mechanism by drawing cleaning repeatedly
Liquid cleans the inside of injection mechanism, and cleaning effect is more preferable, will not impact to reagent and chip to be detected.
Further, the chip fixed mechanism includes wafer support frame, fastener and second temperature control device.
ELISA chip is fixed on wafer support frame by fastener, and second temperature control device is for controlling the temperature of chip.
Wafer support frame will not influence optical signal detecting.
Further, described testing agency's setting is in chip fixed mechanism side, and moveable in the y direction.Detection machine
Structure is driven by Y-direction driving mechanism, and detection can be scanned to chip different location.The Y-direction driving mechanism can be with
It is to drive testing agency to be moved by motor driven screw rod.
Further, the X-axis component drives Z axis component to be moved in X-direction by motor-driven mechanism or cylinder
It is dynamic.
Further, the Z axis component drives injection mechanism to be moved in Z-direction by motor-driven mechanism or cylinder
It is dynamic.
The beneficial effects of the utility model are:
The elisa assay instrument of the utility model is the full-automatic analysis and testing equipment of high integration, has automatic sampling
The functions such as sample-adding, automatic cleaning, temperature automatically controlled, automatic detection, can be realized high-throughput detection, and the utility model design
Optical signal detection mechanism can be improved using the straightline propagation of light and the principle of black material extinction, effective exclusive PCR light
Detection sensitivity and detection accuracy.
Detailed description of the invention
Fig. 1 is the utility model elisa assay instrument complete machine schematic diagram；
Fig. 2 is the structural schematic diagram of the utility model elisa assay instrument testing agency；
Fig. 3 is the schematic illustration of the utility model elisa assay instrument testing agency；
Fig. 4 is the structural schematic diagram of the utility model elisa assay instrument limiting device；
Fig. 5 is the structural schematic diagram of the utility model elisa assay instrument chip fixed mechanism；
Fig. 6 is the structural schematic diagram of the utility model elisa assay instrument injection mechanism.
Specific embodiment
The utility model is described in further detail in the following with reference to the drawings and specific embodiments, so that those skilled in the art
The utility model may be better understood and can be practiced, but illustrated embodiment is not as the restriction to the utility model.
Embodiment 1:
In conjunction with attached drawing, a kind of elisa assay instrument of the utility model, including rack 1；X-axis component 2, Z are installed in rack
Shaft assembly 3, reagent placement mechanism 4, wiper mechanism 5, chip fixed mechanism 6 and testing agency 7；
Z axis component 3 is fixedly mounted on X-axis component 2, can be driven by X-axis component 2 mobile in X-direction；The Z axis group
Injection mechanism 8 is installed, injection mechanism 8 can be driven lower movement in z-direction by Z axis component 3 on part 3；It can be by conjunction with Z axis component
X-axis Component driver is mobile in X-direction, and injection mechanism can be moved in X-direction and Z-direction；
Reagent placement mechanism 4, wiper mechanism 5 and chip fixed mechanism 6 are mounted on the lower section of injection mechanism 8, and reagent
Placement mechanism 4, wiper mechanism 5 and chip fixed mechanism 6 are arranged in X direction；Injection mechanism 8 can be in reagent placement mechanism 4, clear
It is moved between washing machine structure 5 and chip fixed mechanism 6, the operation such as is loaded, is sampled, cleaned；
Testing agency 7 is including laser emitter 71, optical signal detector 72 and is successively set on laser emitter 71 and light
The first loophole 73, filter 74 and the second loophole 75, the first loophole 73 and the second light transmission between signal detector 72
Hole 75 is coaxially disposed, and testing agency 7 is prepared using black material.Chip is placed in laser emitter 71 and the first loophole 73
Between detected.Laser emitter 71 selects the laser emitter of transmitting 450nm light wave, corresponding filter plate in the present embodiment
74 select the filter plate for penetrating 450nm light wave.The testing agency 7 of the utility model further includes laser emitter limiting device 76,
Including third loophole 761 and laser emitter limit hole 762, third loophole 761 and the first loophole 73 are coaxially disposed, and
Diameter is suitable.The light that laser emitter 71 emits is by the injection of third loophole, through chip to be detected, then in turn through the
One loophole 73, optical filter 74 and second penetrate hole 75, reach optical signal detector 72 and are detected.It is specific to reduce interference light
The schematic diagram of line is shown in Fig. 3, and according to the straightline propagation principle of light, the round of external disturbing rays closest to detector is along figure
Route 1 is propagated in 3, is then absorbed by black material, cannot be introduced into optical signal detector；In addition, being formed by diffusing reflection
Some light, the light only between route 2 and route 3 is possible to enter optical signal detector, most of also
Into optical signal detector, and the light between route 2 and route 3, it is filtered out there are also part by filter plate, to testing result
Influence it is very small, by above-mentioned design, influence of the extraneous light to the result of detection is reduced to minimum.
The setting of testing agency 7 of the utility model is and moveable in the y direction in 6 side of chip fixed mechanism.Testing agency
7 are driven by Y-direction driving mechanism, and detection can be scanned to chip different location.Y-direction driving mechanism, which can be, passes through electricity
Machine drives screw rod, and testing agency is driven to be moved.
The reagent placement mechanism 4 of the utility model includes that the first temperature control equipment 41, reagent orifice plate 42 and setting are trying
Lid 43 on agent orifice plate 42；Lid 43 can be mobile in X-direction, convenient to protect to reagent.Reagent orifice plate 42 can be 96
Orifice plate, the first temperature control equipment can be realized 2 DEG C~25 DEG C of accurate control, realize the further protection to reagent.
The wiper mechanism 5 of the utility model includes rinse bath and spray equipment.Cleaning solution is pumped into spray equipment, is passed through
The mode of partial high pressure spray cleaning outside injection mechanism to cleaning, while injection mechanism is by drawing cleaning solution repeatedly,
The inside of injection mechanism is cleaned, cleaning effect is more preferable, will not impact to reagent and chip to be detected.
The chip fixed mechanism 6 of the utility model includes that wafer support frame 61, fastener 62 and second temperature control device (are set
It sets on the inside of bracket, is not shown in figure).ELISA chip is fixed on wafer support frame 61 by fastener 62, second temperature control
Device 63 controls the temperature of chip, settable incubation temperature.Wafer support frame will not influence optical signal detecting.
Embodiment described above is only preferred embodiments for fully illustrating the utility model, the utility model
Protection scope it is without being limited thereto.Those skilled in the art made equivalent substitute or change on the basis of the utility model
It changes, both is within the protection scope of the present invention.The protection scope of the utility model is subject to claims.
Claims (9)
1. a kind of elisa assay instrument, including rack；It is characterized in that,
X-axis component, Z axis component, reagent placement mechanism, wiper mechanism, chip fixed mechanism and detection are installed in the rack
Mechanism；
The Z axis component is fixedly mounted on X-axis component, can be mobile in X-direction by X-axis Component driver；The Z axis component
On injection mechanism is installed, the injection mechanism can be by the lower movement in z-direction of Z axis Component driver；
Reagent placement mechanism, wiper mechanism and the chip fixed mechanism is mounted on the lower section of injection mechanism, and reagent is put
Mechanism, wiper mechanism and chip fixed mechanism is set to arrange in X direction；
The testing agency includes laser emitter, optical signal detector and is successively set on laser emitter and optical signal and examines
The first loophole, filter and the second loophole between device are surveyed, first loophole and the second loophole are coaxially disposed,
The testing agency is prepared using black material.
2. a kind of elisa assay instrument according to claim 1, which is characterized in that the filter is to send out through laser
The filter of emitter launch wavelength.
3. a kind of elisa assay instrument according to claim 1, which is characterized in that the testing agency further includes laser
Transmitter limiting device, the laser emitter limiting device include third loophole and laser emitter limit hole, described
Third loophole and the first loophole be coaxially disposed.
4. a kind of elisa assay instrument according to claim 1, which is characterized in that the reagent placement mechanism includes the
One temperature control equipment, orifice plate and the lid being arranged on reagent orifice plate；The lid can be mobile in X-direction.
5. a kind of elisa assay instrument according to claim 1, which is characterized in that the wiper mechanism includes rinse bath
And spray equipment.
6. a kind of elisa assay instrument according to claim 1, which is characterized in that the chip fixed mechanism includes core
Piece support frame, fastener and second temperature control device.
7. a kind of elisa assay instrument according to claim 1, which is characterized in that the testing agency is arranged in chip
Fixed mechanism side, and it is moveable in the y direction.
8. a kind of elisa assay instrument according to claim 1, which is characterized in that the X-axis component passes through motor driven
Mechanism or cylinder driving Z axis component are moved in X-direction.
9. a kind of elisa assay instrument according to claim 1, which is characterized in that the Z axis component passes through motor driven
Mechanism or cylinder driving injection mechanism are moved in Z-direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822252466.7U CN209559739U (en) | 2018-12-29 | 2018-12-29 | A kind of elisa assay instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822252466.7U CN209559739U (en) | 2018-12-29 | 2018-12-29 | A kind of elisa assay instrument |
Publications (1)
| Publication Number | Publication Date |
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| CN209559739U true CN209559739U (en) | 2019-10-29 |
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| CN201822252466.7U Active CN209559739U (en) | 2018-12-29 | 2018-12-29 | A kind of elisa assay instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109520930A (en) * | 2018-12-29 | 2019-03-26 | 苏州汶颢微流控技术股份有限公司 | A kind of elisa assay instrument |
-
2018
- 2018-12-29 CN CN201822252466.7U patent/CN209559739U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109520930A (en) * | 2018-12-29 | 2019-03-26 | 苏州汶颢微流控技术股份有限公司 | A kind of elisa assay instrument |
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