CN209449420U - A kind of inoculation tissue block culture bottle - Google Patents
A kind of inoculation tissue block culture bottle Download PDFInfo
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- CN209449420U CN209449420U CN201920095740.6U CN201920095740U CN209449420U CN 209449420 U CN209449420 U CN 209449420U CN 201920095740 U CN201920095740 U CN 201920095740U CN 209449420 U CN209449420 U CN 209449420U
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Abstract
The utility model discloses a kind of inoculation tissue block culture bottles, including bottle body and culture plate, the bottleneck of the bottle body is plugged with bottle stopper, water diversion disk is combined with below the bottle stopper, the lower surface two sides of the water diversion disk are respectively and fixedly provided with water-supply-pipe, the centre of the culture plate is provided through culture capsule, and culture plate is fixed on water-supply-pipe, the culture plate is arranged along the vertical direction equidistant parallel of water-supply-pipe, the inner wall of the bottle body is fixedly arranged above lamp source, and the side wall of bottle body equidistantly runs through inoculation mouth, the interior bottom portion of the bottle body is fixed with optical reflective disk, running through outside the upper end of the water-supply-pipe has drainage capillary, and the end for draining capillary is connected with evaporation tube, it is electrically connected between the evaporation tube and lamp source by conducting wire, the outer wall of the bottle body is provided with chopping mechanism.The inoculation tissue block culture bottle is internally provided with layered structure, can be completed at the same time the culture of multiple tissue blocks, and inoculation is convenient, and water in bottle point supplies uniform and appropriateness.
Description
Technical field
The utility model relates to uterus tissue pieces bottle technical field, specially a kind of inoculation tissue block culture bottle.
Background technique
Plant Tissue Breeding refers to plant parts tissue, such as forming layer, parenchymal tissue, mesophyll tissue, endosperm progress
Culture obtain regeneration plant, also refer to during the cultivation process from each organ generate callus culture, callus using
It is differentiated to form aftergrowth again, tissue cultures are generally combined with inoculation technique, and tissue cultures need to use allied organization's culture
The devices such as bottle or incubator for tissue culture.
In use, the supply being divided to for water in bottle is extreme there are two kinds, and one is water for existing tissue culture flasks
Divide supply excessively sufficient, tissue cultures is caused to fail, it is very few that one is moisture supplies, causes tissue block by water unevenness, and water shortage is dead
It dies, meanwhile, typically no lamination mechanism in tissue culture flasks leads to a tissue culture flasks only and can complete a kind of tissue block
Culture wastes space in bottle, for this purpose, it is proposed that a kind of inoculation tissue block culture bottle.
Utility model content
The purpose of this utility model is to provide a kind of inoculation tissue block culture bottles, to solve to propose in above-mentioned background technique
Existing tissue culture flasks in use, the supply being divided to for water in bottle is extreme there are two kinds, and one is moisture supplies
Excessively sufficient, it is very few that one is moisture supplies, meanwhile, in tissue culture flasks the problem of typically no lamination mechanism.
To achieve the above object, the utility model provides the following technical solutions: a kind of inoculation tissue block culture bottle, including bottle
Body and culture plate, the bottleneck of the bottle body are plugged with bottle stopper, and water diversion disk, the following table of the water diversion disk are combined with below the bottle stopper
Face two sides are respectively and fixedly provided with water-supply-pipe, and the centre of the culture plate is provided through culture capsule, and culture plate is fixed on water-supply-pipe,
The culture plate is arranged along the vertical direction equidistant parallel of water-supply-pipe, and the inner wall of the bottle body is fixedly arranged above lamp source, and bottle body
Side wall equidistantly run through and have inoculation mouth, the interior bottom portion of the bottle body is fixed with optical reflective disk, passes through outside the upper end of the water-supply-pipe
It is installed with drainage capillary, and the end for draining capillary is connected with evaporation tube, passes through conducting wire electricity between the evaporation tube and lamp source
Property connection, the outer wall of the bottle body is provided with chopping mechanism.
Preferably, the inside center of the bottle stopper, which vertically runs through, runner, and the top center of bottle stopper is equipped with interface, and
And interface is connected with runner.
Preferably, the inner horizontal of the water diversion disk has laterally provided diversion channel, and diversion channel is connected to knot with runner composition
Structure.
Preferably, the water-supply-pipe is internally provided with aqueduct, and aqueduct and diversion channel, aqueduct and drainage capillary
It is connectivity structure between pipe.
Preferably, the inner transverse of the culture plate is provided with penstock, and phase between penstock and the inner cavity of culture capsule
Connection, and be connected between penstock and aqueduct.
Preferably, the chopping mechanism includes support plate, sliding slot and barn door, and the inner wall of support plate equidistantly offers cunning
Slot, and the fit inside of sliding slot has barn door.
Compared with prior art, the utility model has the beneficial effects that
1, the inoculation tissue block culture bottle is internally provided with layered structure, can be completed at the same time the culture of multiple tissue blocks,
Inoculation is convenient, and water in bottle point supply is uniformly and appropriateness, bottle body use glass material, has extraordinary translucency and transparency,
Space in bottle is separated out multilayer space by multiple culture plates, cultivates multiple groups tissue block with the same time, culture capsule is for holding tissue
Block, water-supply-pipe are used to supply nutrient source for each culture plate, are gone back in addition through there is a drainage capillary on water-supply-pipe, drain hair
Tubule directs the water in water-supply-pipe to evaporation tube, and evaporation tube is to lead to using heating wire as the ceramic sleeve pipe structure of inner core material
High temperature will be generated after electricity, be then evaporated the moisture for evaporating pipe outer wall contamination, it is wet in bottle so as to directly improve
Degree, and moisture is more uniformly spread in the bottle under which, lamp source is used for for light, and reflective panel surface is coated with reflective layer, can
Light is reflected, to be carried out to the tissue block of lower section good for light.
2, bottle stopper clogs bottleneck, guarantees the closing of bottle body inner space, passes through interface, it is convenient to inject nutrition into runner
Source, nutrient source are entered in the diversion channel in water diversion disk by runner, and are distributed in each water-supply-pipe by diversion channel, and nutrient source exists
It flows downward in aqueduct, and is flowed into each culture plate via each penstock, is finally pooled in each culture capsule, to be group
The culture for knitting block provides good nutrient environment.
3, the culture bottle outer wall is provided with chopping mechanism, can flexibly be covered to bottle UV light environment, barn door is matched
It closes in sliding slot, can carry out revolution sliding in sliding slot, and barn door is provided with multiple, presses in several barn doors head and the tail
When, area shading can be completed, when several barn doors are completely overlapped, large area will not be carried out to light again and blocked, it should
Item setting, convenient for light sensation environment locating for building culture bottle entirety.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is the utility model water-supply-pipe and culture plate attachment structure schematic diagram；
Fig. 3 is enlarged structure schematic diagram at A in the utility model Fig. 1；
Fig. 4 is enlarged structure schematic diagram at B in the utility model Fig. 2.
In figure: 1, bottle body；2, bottle stopper；201, runner；202, interface；3, water diversion disk；301, diversion channel；4, water-supply-pipe；
401, aqueduct；5, culture plate；501, penstock；502, capsule is cultivated；6, lamp source；7, inoculation mouth；8, optical reflective disk；9, capillary is drained
Pipe；10, evaporation tube；11, chopping mechanism；1101, support plate；1102, sliding slot；1103, barn door.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
The utility model provides a kind of technical solution referring to FIG. 1 to FIG. 4: a kind of inoculation tissue block culture bottle, including bottle
Body 1 and culture plate 5, the bottleneck of bottle body 1 are plugged with bottle stopper 2, and the inside center of bottle stopper 2, which vertically runs through, runner 201, and bottle stopper 2
Top center is equipped with interface 202, and interface 202 is connected with runner 201, and bottle stopper 2 clogs bottleneck, guarantees inside bottle body 1
Space-closed passes through interface 202, it is convenient to nutrient source is injected in Xiang Liudao 201, the lower section of bottle stopper 2 is combined with water diversion disk 3,
The lower surface two sides of water diversion disk 3 are respectively and fixedly provided with water-supply-pipe 4, and the inner horizontal of water diversion disk 3 has laterally provided diversion channel 301, and divides
Water channel 301 and runner 201 constitute connectivity structure, and nutrient source is entered in the diversion channel 301 in water diversion disk 3 by runner 201, again
It is distributed in each water-supply-pipe 4 by diversion channel 301, the centre of culture plate 5 is provided through culture capsule 502, and culture plate 5 is fixed on
On water-supply-pipe 4, culture plate 5 is arranged along the vertical direction equidistant parallel of water-supply-pipe 4, and space in bottle is separated out by multiple culture plates 5
Multiple groups tissue block is cultivated with the same time in multilayer space, and culture capsule 502 is used to be each culture for holding tissue block, water-supply-pipe 4
Disk 5 supplies nutrient source；
The inner wall of bottle body 1 is fixedly arranged above lamp source 6, and the side wall of bottle body 1 equidistantly runs through inoculation mouth 7, the inside of bottle body 1
Bottom is fixed with optical reflective disk 8, and 8 surface of optical reflective disk is coated with reflective layer, can reflect light, thus to the tissue of lower section
Block progress is good for light, and the upper end outside of water-supply-pipe 4, which is run through, drainage capillary 9, and the end for draining capillary 9 is connected with
Evaporation tube 10, water-supply-pipe 4 are internally provided with aqueduct 401, and aqueduct 401 and diversion channel 301, aqueduct 401 and drainage
It is connectivity structure between capillary 9, drainage capillary 9 directs the water in water-supply-pipe 4 to evaporation tube 10, and evaporation tube 10 is
Using heating wire as the ceramic sleeve pipe structure of inner core material, high temperature will be generated after energization, then by the contamination of 10 outer wall of evaporation tube
Moisture is evaporated, and so as to directly improve the humidity in bottle, and moisture is more uniformly spread in the bottle under which, culture
The inner transverse of disk 5 is provided with penstock 501, and is connected between penstock 501 and the inner cavity of culture capsule 502, and diversion
It is connected between road 501 and aqueduct 401, nutrient source flows downward in aqueduct 401, and flows into via each penstock 501
It into each culture plate 5, is finally pooled in each culture capsule 502, so that the culture for tissue block provides good nutrient environment, steams
It sends out and is electrically connected between pipe 10 and lamp source 6 by conducting wire；
The outer wall of bottle body 1 is provided with chopping mechanism 11, and chopping mechanism 11 includes support plate 1101, sliding slot 1102 and barn door
1103, and the inner wall of support plate 1101 equidistantly offers sliding slot 1102, and the fit inside of sliding slot 1102 has barn door 1103,
The culture bottle outer wall is provided with chopping mechanism 11, can flexibly be covered to bottle UV light environment, and the cooperation of barn door 1103 exists
In sliding slot 1102, revolution sliding can be carried out in sliding slot 1102, and barn door 1103 be provided with it is multiple, in several barn doors
When 1103 head and the tail press, area shading can be completed, when several barn doors 1103 are completely overlapped, light will not be carried out again
Large area is blocked, this setting, convenient for light sensation environment locating for building culture bottle entirety.
Working principle: for this kind of culture bottle, inoculating gun is extend into bottle body 1 by inoculation mouth 7 first, by tissue
Block is sent to culture 502 position of capsule, and tissue block is placed in culture capsule 502, the runner 201 by interface 202, into bottle stopper 2
Nutrient source is conveyed, nutrient source is entered in the diversion channel 301 in water diversion disk 3 by runner 201, and is distributed to by diversion channel 301
In each water-supply-pipe 4, nutrient source flows downward in aqueduct 401, and is flowed into each culture plate 5 via each penstock 501, most
After be pooled in each culture capsule 502, to provide good nutrient environment for tissue block, nutrient source is the dilution of water and nutrient solution
Object during nutrient source flows downward in aqueduct 401, separates a part to steaming by draining capillary 9 containing moisture
It sends out on pipe 10, after the energization of lamp source 6 is lighted, evaporation tube 10 can also be powered, and the heating wire inside evaporation tube 10 is in the state of energization
Heat is begun to send out, the nutrient solution moisture of 10 outer wall of evaporation tube is evaporated, the vapor of evaporation is dispersed in bottle body 1,
So that 1 internal environment of bottle body is in the state of humidity equilibrium, and due to the heating functioin of evaporation tube 10, so that 1 inner loop of bottle body
Border is in temperature constant state, is more advantageous to the culture of tissue block, and optical reflective disk 8 is used to reflect the light of the sending of lamp source 6, so that under
Tissue block in layer culture plate 5 can also obtain good illumination effect, after culture bottle is placed on experiment frame, some group
It knits block to need to be cultivated under more dark environment, each barn door 1103 can be slided manually at this time, it will be several
When barn door 1103 presses from beginning to end, area shading can be completed, it, will not be right again when several barn doors 1103 are completely overlapped
Light carries out blocking for large area, guarantees the natural light transmission of bottle body 1, completes the use process of entire culture bottle like this.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of inoculation tissue block culture bottle, including bottle body (1) and culture plate (5), it is characterised in that: the bottle of the bottle body (1)
Mouth is plugged with bottle stopper (2), is combined with water diversion disk (3) below the bottle stopper (2), the lower surface two sides of the water diversion disk (3) are solid
Surely have water-supply-pipe (4), the centre of the culture plate (5) is provided through culture capsule (502), and culture plate (5) is fixed on water delivery
It manages on (4), the culture plate (5) is arranged along the vertical direction equidistant parallel of water-supply-pipe (4), above the inner wall of the bottle body (1)
It is fixed with lamp source (6), and the side wall of bottle body (1) equidistantly runs through and has inoculation mouth (7), the interior bottom portion of the bottle body (1) is fixed with
Optical reflective disk (8), the upper end outside of the water-supply-pipe (4), which is run through, to be had drainage capillary (9), and the end for draining capillary (9) connects
It is connected to evaporation tube (10), is electrically connected between the evaporation tube (10) and lamp source (6) by conducting wire, the outer wall of the bottle body (1)
It is provided with chopping mechanism (11).
2. a kind of inoculation tissue block culture bottle according to claim 1, it is characterised in that: in the inside of the bottle stopper (2)
The heart, which vertically runs through, to be had runner (201), and the top center of bottle stopper (2) is equipped with interface (202), and interface (202) and runner
(201) it is connected.
3. a kind of inoculation tissue block culture bottle according to claim 2, it is characterised in that: the inside of the water diversion disk (3)
Horizontal cross has provided diversion channel (301), and diversion channel (301) and runner (201) constitute connectivity structure.
4. a kind of inoculation tissue block culture bottle according to claim 3, it is characterised in that: the inside of the water-supply-pipe (4)
It is provided with aqueduct (401), and between aqueduct (401) and diversion channel (301), aqueduct (401) and drainage capillary (9)
For connectivity structure.
5. a kind of inoculation tissue block culture bottle according to claim 4, it is characterised in that: the inside of the culture plate (5)
It is horizontally arranged with penstock (501), and is connected between penstock (501) and the inner cavity of culture capsule (502), and penstock
(501) it is connected between aqueduct (401).
6. a kind of inoculation tissue block culture bottle according to claim 1, it is characterised in that: the chopping mechanism (11) includes
Support plate (1101), sliding slot (1102) and barn door (1103), and the inner wall of support plate (1101) equidistantly offers sliding slot
(1102), and the fit inside of sliding slot (1102) has barn door (1103).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920095740.6U CN209449420U (en) | 2019-01-21 | 2019-01-21 | A kind of inoculation tissue block culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920095740.6U CN209449420U (en) | 2019-01-21 | 2019-01-21 | A kind of inoculation tissue block culture bottle |
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| Publication Number | Publication Date |
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| CN209449420U true CN209449420U (en) | 2019-10-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201920095740.6U Active CN209449420U (en) | 2019-01-21 | 2019-01-21 | A kind of inoculation tissue block culture bottle |
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| CN (1) | CN209449420U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518692A (en) * | 2020-04-30 | 2020-08-11 | 海口健康岛生物科技有限公司 | Automatic human stem cell culture instrument |
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2019
- 2019-01-21 CN CN201920095740.6U patent/CN209449420U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518692A (en) * | 2020-04-30 | 2020-08-11 | 海口健康岛生物科技有限公司 | Automatic human stem cell culture instrument |
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