CN209338502U - A kind of monocyte separator - Google Patents
A kind of monocyte separator Download PDFInfo
- Publication number
- CN209338502U CN209338502U CN201821661457.7U CN201821661457U CN209338502U CN 209338502 U CN209338502 U CN 209338502U CN 201821661457 U CN201821661457 U CN 201821661457U CN 209338502 U CN209338502 U CN 209338502U
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- China
- Prior art keywords
- monocyte
- support
- supporting block
- separator according
- fixation elements
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Abstract
The utility model relates to a kind of monocyte separators, described device includes separation vessel, support fixation elements and collection vessel, it is characterized in that, the separation vessel is fixed in the collection vessel by the support fixation elements, and the through-hole that aperture is 100um-1000um is disposed on the wall of the separation vessel.Monocyte separator provided by the utility model, it is simple and compact for structure, it can easily and effectively realize the separation dilution to monocyte.
Description
Technical field
The utility model relates to separation technology field more particularly to a kind of monocyte separators.
Background technique
For biggish microorganism, capillary can be used and extract single individual, and is shifted in a large amount of sterilising medium
Cleaning several times, removes the pollution of smaller microorganism.This operation can be in low-powered microscope, as carried out under disecting microscope.
Microorganism relatively small for individual need to use micromanipulation instrument, carry out under the microscope.Currently, in the market
There are many micromanipulation instrument type on sale, reduce the movement range of hand generally by mechanical, air or oil hydraulic actuator,
Under the microscope with the pickings such as capillary or micro-needle, hook, ring single microbial cell or spore to obtain pure culture.
In no micromanipulation instrument, the method that some accommodations can also be used carries out unicellular separation, example under the microscope
Such as, the sample preparation after suitably diluting is observed under the microscope at droplet, chooses the drop containing only a cell and carries out
Culture is to obtain pure culture.Unicellular partition method has relatively high requirement to operating technology, is limited to the science of highly-specialised more
It is used in research.The apparatus structure complexity of unicellular selection is carried out by preparing drop at present, it is inconvenient for operation, it is therefore necessary to
A kind of monocyte separator that structure is simple to operation is provided.
Utility model content
The utility model is intended to provide a kind of monocyte separator, to simplify structure, so that monocyte separation is dilute
It is simple and easy to release operating process.
Monocyte separator provided by the utility model mainly includes separation vessel, support fixation elements and collection
Container, wherein support fixation elements are for separation vessel to be supported and fixed in collection vessel.Cloth on the wall of the separation vessel
It is equipped with the through-hole that aperture is 100um-1000um, is passed through for monocyte in separation vessel.
The wall includes side wall and bottom wall；Or the wall is side wall；Or the wall is bottom wall.
Preferably, the through-hole is multiple, and the distribution density of the through-hole is 50-830/cm2.Preferably, described logical
The cross section that hole is is round.Preferably, the distribution density of the through-hole is 80-500/cm2.Preferably, the through-hole is equal
Even distribution.
The support fixation elements are equipped with inner peripheral surface and outer peripheral surface, and the inner peripheral surface of the support fixation elements is for fastening institute
Separation vessel is stated, the outer peripheral surface of the support fixation elements is for the inner wall tight fit connection with the collection vessel.Alternatively, institute
Support fixation elements are stated equipped with interior stationary plane and end fixation member, the interior stationary plane is for fastening the separation vessel, institute
End fixation member is stated for being fixedly mounted with the upper end of the collection vessel.
" inner peripheral surface ", " outer peripheral surface ", " interior stationary plane " in the utility model are not defined the shape of structure, can be with
For regular shape or irregular shape；Such as " inner peripheral surface ", " outer peripheral surface ", " interior stationary plane " shape can be circle,
It is rectangular etc..
Specifically, the support fixation elements include support rod and supporting block, and the supporting block is set in the support rod
On, the supporting block is equipped with the inner peripheral surface and the outer peripheral surface.
Further, the supporting block is made of elastic material.
Further, the support rod can be one group, such as at least two, this group of support rod arranged for interval is preferably circumferential
Arrangement, this group of support rod are connected in end by installation part.
Above-mentioned separator, the supporting block can be one group, such as at least two, this group of supporting block is along the support rod
Longitudinal direction arranged for interval, be collected into collection vessel bottom for the ease of monocyte and liquid, the supporting block is equipped with
Longitudinally through hole.
Further, the support fixation elements are additionally provided with limit card block, and the limit card block is arranged in the supporting block
Both ends along the longitudinal direction, it is preferable that two that the supporting block extends along the support rod length direction are arranged in limit card block
On the support rod at end, to limit moving up and down for supporting block in a longitudinal direction.
The support fixation elements are additionally provided with washer, are located on the outer peripheral surface of the supporting block, to protect collection vessel
Inner wall, in order to avoid be scratched.
Above-mentioned separator, the separator are provided with first seal and use for sealing the separation vessel
In the second seal for closing the collection vessel.
Further, the first seal can be plug or lid.
Further, the second seal can be plug or buckle closure, and the upper end of the support rod is arranged in.When second close
When sealing is plug, for cooperating with the inner wall of the collection vessel to seal the upper end of collection vessel, when second seal is
When buckle closure, the inner wall of buckle closure and the outer wall of collection vessel cooperate to seal the upper end of collection vessel.
The support fixation elements are additionally provided with push-and-pull component.The push-and-pull component is arranged at the top of the upper end of the support rod
Or side, for holding, and then push the support fixation elements that separation vessel is driven to move up and down.
Preferably, non-slip groove is provided on the push-and-pull component.
Preferably, the support rod, the installation part and the push-and-pull component are integrally formed.
Above-mentioned separator, the collection vessel can be test tube.
Monocyte separator provided by the utility model first can be fixed separation vessel by support fixation elements,
It is packed into monocyte solution to be separated in the separation vessel, then support fixation elements are packed into collection vessel together with separation vessel
It is interior, separating liquid body is packed into collection vessel, by the outer peripheral surface of support fixation elements and the inner wall tight fit connection of collection vessel,
Support fixation elements and separation vessel are firmly secured in collection vessel, the monocyte in separation vessel passes through on tube wall
Through-hole through certain time flow into collection vessel in, achieve the purpose that separation dilution monocyte.The utility model overall structure
Simply, operation is easy, and realizes the separation dilution to monocyte with can be convenient.
Detailed description of the invention
Fig. 1 is longitudinal schematic cross-sectional view of the utility model embodiment；
Fig. 2 is the schematic diagram of the section structure of the installation part of the utility model embodiment, supporting block and support rod；
Fig. 3 is the partial enlargement schematic cross-sectional view of the utility model embodiment.
Drawing reference numeral explanation:
1, test tube；2, separation vessel；3, support rod；4, limit card block；5, supporting block；6, washer；7, installation part；8, convex
Block；9, plug one；10, plug two.
Specific embodiment
The utility model is understood and realized for the ease of persons skilled in the art, and it is practical new to describe this now in conjunction with attached drawing
The embodiment of type.
In order in an experiment can using succinct apparatus and can easily realize monocyte separation operation,
Utility model proposes that a kind of structure is simple, easily fixes, does not need to hold separating pipe in the process of monocyte separation
Device need to only be packed into monocyte solution to be separated in separation vessel, and separation vessel is fixed on one and is equipped with later and is divided
In the collection vessel of chaotropic body, so that it may be allowed to rest for realizing the separation of monocyte, and then to the monokaryon entered in separation liquid
Cell is diluted.
Specifically, monocyte separator provided by the utility model, mainly includes separation vessel, support fixation elements
And collection vessel, wherein support fixation elements are for separation vessel to be supported and fixed in collection vessel.The support fixation elements
Structure can be substantially ring-like, be equipped with inner peripheral surface and outer peripheral surface, the inner peripheral surfaces of the support fixation elements is for fastening described point
From container, the outer peripheral surface of the support fixation elements will separate appearance for the inner wall tight fit connection with the collection vessel
Device is securely fixed in collection vessel.Support fixation elements setting inner peripheral surface and outer peripheral surface belong to the first of the utility model
Situation, following the application first will describe to this kind of situation in detail.
Specifically, the through-hole that aperture is 100um-1000um is furnished on separation vessel wall, it is convenient in separation vessel
Monocyte enters in collection vessel after free.Preferably, the cross section of the through-hole is circle.Through-hole setting exists
Separation vessel side wall and bottom wall；Or through-hole is arranged in separation vessel side wall；Or through-hole is arranged in separation vessel bottom wall.
Preferably, through-hole is multiple, and the distribution density of the through-hole can be 50-830/cm2, it is therefore preferable to 80-
500/cm2。
The distribution of through-hole can be uniform or heterogeneous；Preferably, the through-hole is to be uniformly distributed.
More specifically, the support fixation elements may include support rod and supporting block, support rod extends in longitudinal direction, branch
Bracer is set on support rod.Supporting block and support rod can be connected to one piece in a manner of moulding by casting, can also be by making to support
The mode that bar passes through supporting block links together, certainly can also be by connecting the two otherwise.Supporting block in a ring, is supporting
Block is equipped with the inner peripheral surface and the outer peripheral surface.When fixedly separated container, separation vessel can be moved into support along longitudinal direction and fixed
The inner peripheral surface of component forms tight fit, and then support fixedly separated appearance so that the outer wall of inner peripheral surface and separation vessel is in close contact
Device.Preferably, supporting block can be used elastic material and be made, for example rubber material is made, and move in this way convenient for the immigration of separation vessel
Out.Certainly, elastic card can also be set in inner peripheral surface, by elastic force clamping separation vessel.Similarly, individually size can also be taken to match
The approach of conjunction makes separation vessel and inner peripheral surface form tight fit relationship.Similar, it can also be arranged on the inner wall of collection vessel
Elastic card, by elastic card compact schemes fixation member.Equally, the approach of dimensional fits can also be individually taken to make collection vessel
Tight fit relationship is formed with outer peripheral surface.
Preferably, the support rod is one group, is arranged side by side in a longitudinal direction, in circumferentially spaced-apart arrangement.Preferably, should
Group support rod is connected in end by installation part.Installation part is all settable at both ends, as long as the immigration of separation vessel is not interfered to move
Out.
Also there is no limit can be designed to be enough to undertake the support to separation vessel with fastening i.e. to the quantity of supporting block
It can.Preferably, supporting block is designed as one group, longitudinal direction arranged for interval of this group of supporting block along the support rod.For the ease of
The monocyte in collection vessel and the smooth bottom for being passed through collection vessel of liquid above supporting block are flowed into from separation vessel,
Supporting block is equipped with longitudinal through hole.
Supporting block slides up and down on support rod in order to prevent, influences the fixation to separation vessel, can further support
Limit card block is set in fixation member.Preferably, the both ends of supporting block along the longitudinal direction are arranged in limit card block, in longitudinal side
Moving up and down for supporting block is limited upwards.
Preferably, support fixation elements are additionally provided with washer, are set on the outer peripheral surface of supporting block, to protect collection vessel
Inner wall, in order to avoid be scratched.Also with no restriction, one or more washers can be arranged in the quantity of washer in every piece of supporting block, should
In the card slot of the embeddable supporting block of washer.If a washer is arranged in every piece of supporting block, the centre of supporting block may be provided at
Position.
In order to which during monocyte separates, the integrated environment of separator can not be influenced by the external world, the effect of separation is ensured
Fruit, separator are additionally provided with sealing element, for the hermetic separation container after separation vessel to be placed into collection vessel and the receipts
Collect the upper port of container.Generally, after the support fixation elements for being clamped with separation vessel are put into collection vessel, support is fixed
The upper end of component can be higher by collection vessel a part, to carry out moving operation to support fixation elements.It therefore, can be close by second
The upper end of support fixation elements is arranged in sealing.For example, second seal may be designed as plug or buckle closure, the plug or buckle closure are worn
Cross the support rod of support fixation elements, outside edge contacts collection vessel, to form separation vessel in the upper end of collection vessel and outer
Boundary and collection vessel itself and extraneous sealing.When second seal is plug, the outer rim of the plug is used for and collects appearance
The inner wall of device cooperates with the upper end of sealing device.When second seal is buckle closure, the inner wall of buckle closure and the outer wall of collection vessel
Cooperation is with the upper end of sealing device.
Preferably, the separator is additionally provided with first seal such as plug or lid, for tight with the separation vessel
Cooperation, independently forms sealing to separation vessel.
For the ease of the operation and gripping to support fixation elements, the support fixation elements are additionally provided with push-and-pull component, should
Push-and-pull component may be provided at the upper end of support fixation elements, for example the top or side of post upper is arranged in, and can be convex
The structure type of block or the structure type of handle for holding, and then push support fixation elements that separation vessel is driven to collect
It is moved up and down in container.For the ease of holding, it is preferable that be provided with non-slip groove on the push-and-pull component.
Preferably, the support rod, the installation part and the push-and-pull component are integrally formed.Preferably, installation part, support
Bar and push-and-pull component are the integrated molding of ABS engineering plastics.
Preferably, the collection vessel in the present embodiment can be test tube, can also be other be suitable for and inside support fixation certainly
Other containers that component is used cooperatively.
When carrying out monocyte separation, first the injection separation liquid in collection vessel, will then be fixed with support
The separation vessel that component has fastened is inserted into collection vessel, and the solution of the monocyte of culture is injected into separation vessel,
First seal is then covered or filled in above separation vessel, push-and-pull component is continued to move to, by equipment mounted thereto
Continue to move down, the second seal in support fixation elements is facilitated to fill in or cover on the upper edge of collection vessel, is formed whole close
Envelope.After the regular hour, push-and-pull component is moved upward out, separation vessel is extracted out, then pours out the liquid in collection vessel
Body completes isolation by dilution method to the separation dilution step of individual cells.In this way, single after free
Nucleus, which enters the number in collection vessel, to meet the demand of dilution method far below the number in separation vessel.
Another scheme of the utility model, the support fixation elements are equipped with interior stationary plane and end fixation member, institute
It states interior stationary plane and is used for and the fixed peace in the upper end of the collection vessel for fastening the separation vessel, the end fixation member
Dress.In this case, support fixation elements are by may be designed as the interior stationary plane of inner ring come fixedly separated container, without passing through
Outer peripheral surface is fixed with collection vessel, but cooperates fixation by the upper end that lid or plug and collection vessel is arranged in end, such as
It when for lid, is coupled with the end of collection vessel upper end and outer wall, when being plug, cooperates with the inner wall of collection vessel and pacify
Dress, and then separation vessel is fixedly secured on collection vessel.At this point, the above-mentioned second seal described in the text of the utility model
It can be used as the end fixation member of this kind of situation, the above-mentioned inner peripheral surface described in the text of the utility model can be used as the interior of this kind of situation
Stationary plane, other than it can be not provided with the outer peripheral surface of support fixation elements and through hole in supporting block, other structures all can be with
The structure of the first situation is identical in above-mentioned text.
Embodiment 1
If Fig. 1-3 is the structural schematic diagram of the present embodiment.Certain attached drawing is used only as exemplary illustration, for the ease of subsequent reality
The explanation of example is applied, not component all in figure is all the necessary setting of the present embodiment.The monocyte of the embodiment separates dress
It sets, including test tube 1, serves as the collection vessel of the utility model.There is separation vessel 2 in test tube 1, is cased on the outside of separation vessel 2
Supporting block 5, supporting block 5 are mounted on support rod 3, and 3 top of support rod is installation part 7, is connected with convex block 8 above installation part 7, convex
Push-and-pull component of the block 8 as the utility model.Plug 1 is plugged on separation vessel 2, the first sealing as separation vessel
Part.Plug 2 10 is cased on installation part 7 and support rod 3.Second seal of the plug 2 10 as the utility model.
Installation part 7, support rod 3 and the convex block 8 of the monocyte separator of the present embodiment can be ABS engineering plastics one
Body formed, this three is the structure of integral type.Push-and-pull component of the convex block 8 as the utility model.For the stabilization of structure, support
The quantity of bar 3 is preferably 3, and is uniformly arranged on installation part 7.Supporting block 5 is cased on support rod 3, supporting block 5 is adopted
It is manufactured with rubber, with certain elasticity.As shown in Fig. 2, supporting block 5 can be made of Internal and external cycle in the present embodiment.Supporting block 5
Inner sidewall contacted with separation vessel 2, realize and the clamping of separation vessel 2 installed.The outside of supporting block 5 and the inner wall of test tube 1
External fixation is realized in contact.In this way after installing, it is achieved that the fixed operation to separation vessel 2.
Small through hole, aperture 100um-1000um, distribution density 50- are equably placed on the outer wall of separation vessel 2
830/cm2, conveniently entered in test tube 1 after free in the monocyte in separation vessel 2.Carrying out separation monokaryon
When cell, first mounted separation vessel 2, is then inserted into test tube 1, will train by the injection separation liquid in test tube 1
The solution of feeding monocyte is injected into separation vessel 2, is then filled in plug 1 in the top of separation vessel 2, is continued to press on
Equipment mounted thereto is continued to move down by convex block 8, and plug 2 10 is facilitated to fill in the upper edge of test tube 1.
After the regular hour, convex block 8 is twitched, separation vessel 2 is extracted out, then pours out the liquid in test tube 1, i.e.,
Complete the dilution step of isolation by dilution method.In this way, after free, monocyte is entered in test tube 1
Quantity will far below enter separation vessel 2 in quantity, meet the needs of dilution method.Due to the through-hole pole on separation vessel 2
Its is small, and only after prolonged dissociate, monocyte is possible to enter in test tube 1.In extraction separation vessel 2
When, using the own tension of liquid, the through-hole on separation vessel 2 there will not be liquid outflow.
Embodiment 2
Another embodiment according to the present utility model, in addition to other than the above-mentioned identical structure of embodiment 1, further,
The outside of supporting block 5 is cased with washer 6.
Washer 6 in supporting block 5 contacts the inner wall of test tube 1, plays the inner wall of protection test tube 1, avoids the case where scratching hair
It is raw.
Embodiment 3
Another embodiment according to the present utility model, in addition to other than the above-mentioned identical structure of embodiment 2, further,
The upper and lower ends of supporting block 5 are fitted with limit card block 4.
Limit card block 4 and installation part 7, support rod 3 and convex block 8 are all integrally formed for ABS engineering plastics, limit card block 4
It is stuck in the two sides up and down of supporting block 5, the feelings for avoiding separation vessel 2 that supporting block 5 is caused to slide up and down when extending into test tube 1
Condition occurs.
Embodiment 4
In addition to other than the above-mentioned identical structure of embodiment 3, another embodiment according to the present utility model, further,
There is non-slip groove on convex block 8.
Non-slip groove increases frictional force, facilitates the operation of experimenter's plug separation vessel 2.
In addition, in the above-described embodiments, the through-hole of perforation is preferably designed in supporting block 5, facilitate the liquid flow in test tube 1
Dynamic, when extracting separation vessel 2 out, liquid can be flowed into the bottom of test tube 1 along the through-hole of perforation, convenient for the receipts of liquid
Collection.
Found out by above-described embodiment, the utility model overall structure is simple, and operation is easy, and realizes to monokaryon with can be convenient
The separation of cell dilutes.
Although depicting the utility model by embodiment, it will be appreciated by the skilled addressee that not departing from this reality
In the case where with novel spirit and essence, so that it may make the utility model there are many deformation and variation, it all should be in the utility model
Protection scope in.
Claims (16)
1. a kind of monocyte separator, described device includes separation vessel, support fixation elements and collection vessel, feature
It is, the separation vessel is fixed in the collection vessel by the support fixation elements, on the wall of the separation vessel
It is disposed with the through-hole that aperture is 100um-1000um.
2. monocyte separator according to claim 1, which is characterized in that the wall includes side wall and bottom wall；Or
Wall described in person is side wall；Or the wall is bottom wall.
3. monocyte separator according to claim 1, which is characterized in that the through-hole is multiple, the through-hole
Cross section be circle, the distribution density of the through-hole is 50-830/cm2。
4. monocyte separator according to claim 3, which is characterized in that the distribution density of the through-hole is 80-
500/cm2。
5. monocyte separator according to claim 3, which is characterized in that the through-hole is uniformly distributed.
6. monocyte separator according to any one of claims 1-5, which is characterized in that the support fixed part
Part is equipped with inner peripheral surface and outer peripheral surface, and for the inner peripheral surface of the support fixation elements for fastening the separation vessel, the support is solid
The outer peripheral surface for determining component is used to install with the inner wall tight fit of the collection vessel；Alternatively, the support fixation elements are equipped with interior
Stationary plane and end fixation member, the interior stationary plane for fastening the separation vessel, the end fixation member be used for
The upper end of the collection vessel is fixedly mounted.
7. monocyte separator according to claim 6, which is characterized in that the support fixation elements include support
Bar and supporting block, the supporting block are set on the support rod, and the inner peripheral surface and the outer peripheral surface are arranged in the support
On block.
8. monocyte separator according to claim 7, which is characterized in that the supporting block is elastic material system
At.
9. monocyte separator according to claim 7, which is characterized in that the support rod at least two, it should
Support rod arranged for interval, support boom end are fixed on installation part；The supporting block at least two, the supporting block is along the branch
The length direction arranged for interval of strut；The supporting block is equipped with longitudinally through hole.
10. monocyte separator according to claim 7, which is characterized in that the support fixation elements are additionally provided with
The branch of the supporting block along the both ends that the support rod length direction extends is arranged in limit card block, the limit card block
On strut.
11. monocyte separator according to claim 7, which is characterized in that the support fixation elements are additionally provided with
Washer, the washer are located on the outer peripheral surface of the supporting block.
12. monocyte separator according to claim 1-5, which is characterized in that the separator is set
It is equipped with the first seal for sealing the separation vessel and the second seal for closing the collection vessel.
13. according to the described in any item monocyte separators of claim 7-11, which is characterized in that the support fixed part
Part is additionally provided with push-and-pull component, and the push-and-pull component is arranged at the top of the upper end of the support rod or side.
14. monocyte separator according to claim 13, which is characterized in that be provided on the push-and-pull component anti-
Sliding slot.
15. monocyte separator according to claim 9, which is characterized in that the support fixation elements are additionally provided with
Component is pushed and pulled, the push-and-pull component is arranged at the top of the upper end of the support rod or side.
16. monocyte separator according to claim 15, which is characterized in that the support rod, the installation part
It is integrally formed with the push-and-pull component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821661457.7U CN209338502U (en) | 2018-10-12 | 2018-10-12 | A kind of monocyte separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821661457.7U CN209338502U (en) | 2018-10-12 | 2018-10-12 | A kind of monocyte separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209338502U true CN209338502U (en) | 2019-09-03 |
Family
ID=67746376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821661457.7U Expired - Fee Related CN209338502U (en) | 2018-10-12 | 2018-10-12 | A kind of monocyte separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209338502U (en) |
-
2018
- 2018-10-12 CN CN201821661457.7U patent/CN209338502U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190903 Termination date: 20201012 |