CN209166965U

CN209166965U - Protein desalter

Info

Publication number: CN209166965U
Application number: CN201821729346.5U
Authority: CN
Inventors: 章会兵; 孔德志
Original assignee: Shijiazhuang Pei Ou Biological Technology Co Ltd
Current assignee: Shijiazhuang Pei Ou Biological Technology Co Ltd
Priority date: 2018-10-23
Filing date: 2018-10-23
Publication date: 2019-07-26
Anticipated expiration: 2028-10-23

Abstract

The utility model provides a kind of protein desalter, belong to protein desalting technology field, including first sleeve, the second casing, enrichment component and air blowing component, second casing non-tight is arranged in first sleeve, waste liquid storage space is equipped between the bottom of second casing and the bottom of first sleeve, the bottom of the second casing is equipped with first through hole；It is enriched with enrichment of the component setting in the second casing, for protein；The upper end of air blowing component and the second casing is tightly connected, and is used for second set of treacheal gas insufflation.Protein desalter provided by the utility model, by air blowing component to second set of treacheal gas insufflation, increase by the second inside pipe casing air pressure, protein solution is flow in waste liquid storage space under gas pressure by enrichment component and first through hole, proteins and peptides are collected in enrichment component, the purification for realizing protein, avoids the drawbacks of original technology is by centrifuge, has the advantages that easy to operate, cost performance is high.

Description

Protein desalter

Technical field

The utility model belongs to protein desalting technology field, is to be related to a kind of protein desalter more specifically.

Background technique

Protein, peptide fragment can adulterate more salts substances in process of production, therefore the needs pair in laboratory research Protein carries out desalination purification, it is therefore an objective to keep apart other salt micromoleculars in protein, peptide fragment.

Commonly used laboratory is desalination centrifugal column, protein solution is added in desalination centrifugal column, in centrifuge Upper carry out centrifugal screening.Since such desalination process must be used cooperatively with centrifuge, operate comparatively laborious, expensive.

Utility model content

The purpose of this utility model is to provide a kind of protein desalters, to solve needs existing in the prior art By problem cumbersome, expensive caused by centrifuge.

To achieve the above object, the utility model provides a kind of protein desalter, comprising:

First sleeve；

Second casing, non-tight are arranged in the first sleeve, the bottom of second casing and the first sleeve Bottom between be equipped with waste liquid storage space, the bottom of second casing is equipped with first through hole；

It is enriched with component, the enrichment in second casing, for protein is set；

Air blowing component is tightly connected with the upper end of second casing, is used for second set of treacheal gas insufflation.

Further, the air blowing component includes that the outer tube being tightly connected with the upper end of second casing and setting exist Piston rod in the outer tube.

Further, the lower end of the outer tube is equipped with the threaded joints connecting with second casing threads.

Further, the upper end of second casing is equipped with the first outer, and the upper end of the threaded joints is equipped with second Outer is equipped with sealing element between first outer and second outer.

Further, the lower surface of the upper surface of first outer and second outer is equipped with identical annular groove, Sealing ring is equipped between two annular grooves.

Further, the enrichment component include the isolation sieve plate that is arranged in second casing and setting it is described every From the filler above sieve plate.

Further, the filler is C18 bonded silica gel filler, C4 bonded silica gel filler or HILIC filler One of.

Further, the aperture of the isolation sieve plate is 20-25 microns.

Further, the isolation sieve plate and second sleeve interference cooperate.

Further, the bottom of the first sleeve is equipped with the second through-hole, and second through-hole is equipped with sealing-plug.

The beneficial effect of protein desalter provided by the utility model is: compared with the prior art, this is practical new The protein desalter that type provides, sealed set has air blowing component on the second casing, and protein to be purified is placed on the second casing It is interior, by air blowing component to second set of treacheal gas insufflation, increase by the second inside pipe casing air pressure, protein solution passes through under gas pressure It crosses enrichment component and first through hole is flow in waste liquid storage space, proteins and peptides are collected in enrichment component, salts substances As solution enters in waste liquid storage space, the purification of protein is realized, avoid the drawbacks of original technology is by centrifuge, have Have the advantages that easy to operate, cost performance is high.

Detailed description of the invention

It, below will be to embodiment or the prior art in order to illustrate more clearly of the technical scheme in the embodiment of the utility model Attached drawing needed in description is briefly described, it should be apparent that, the accompanying drawings in the following description is only that this is practical new Some embodiments of type for those of ordinary skill in the art without creative efforts, can be with root Other attached drawings are obtained according to these attached drawings.

Fig. 1 is the sectional view of protein desalter provided by the embodiment of the utility model；

Fig. 2 is the partial enlarged view in Fig. 1 at A.

Wherein, each appended drawing reference in figure:

1- first sleeve；The second through-hole of 11-；The second casing of 2-；21- first through hole；The first outer 22-；The storage of 3- waste liquid Space；4- is enriched with component；Sieve plate is isolated in 41-；42- filler；5- air blowing component；51- outer tube；511- threaded joints； The second outer 512-；52- piston rod；6- annular groove；7- sealing ring；8- sealing-plug.

Specific embodiment

In order to which technical problem to be solved in the utility model, technical solution and beneficial effect is more clearly understood, with Lower combination accompanying drawings and embodiments, the present invention will be further described in detail.It should be appreciated that specific reality described herein It applies example to be only used to explain the utility model, is not used to limit the utility model.

Refering to fig. 1, now protein desalter provided by the utility model is illustrated.Protein desalter, packet First sleeve 1, the second casing 2, enrichment component 4 and air blowing component 5 are included, 2 non-tight of the second casing is arranged in first sleeve 1, Waste liquid storage space 3 is equipped between the bottom of second casing 2 and the bottom of first sleeve 1, the bottom of the second casing 2 is equipped with first Through-hole 21；Component 4 is enriched with to be arranged in the second casing 2；The upper end of air blowing component 5 and the second casing 2 is tightly connected, for the It blows in two casings 2.

Protein desalter provided by the utility model, sealed set, which has, compared with prior art, on the second casing 2 blows Pneumatic module 5, protein to be purified are placed in the second casing 2, are blown by air blowing component 5 into the second casing 2, increase by second set 2 air pressure inside of pipe, protein solution flow to waste liquid storage space 3 by enrichment component 4 and first through hole 21 under gas pressure Interior, proteins and peptides are collected in enrichment component 4, and salts substances enter in waste liquid storage space 3 with solution, realize albumen The purification of matter avoids the drawbacks of original technology is by centrifuge, has the advantages that easy to operate, cost performance is high.

As a kind of specific embodiment of protein desalter provided by the utility model, refering to fig. 1, air blowing component 5 include the outer tube 51 being tightly connected with the upper end of the second casing 2 and the piston rod 512 being arranged in outer tube 51.Pass through to Interior push piston bar 512 can increase the air pressure in the second casing 2, and under gas pressure, protein solution is by enrichment 4 He of component First through hole 21 is flow in waste liquid storage space 3, and proteins and peptides are collected in enrichment component 4, realizes the desalination of protein.

As a kind of specific embodiment of protein desalter provided by the utility model, refering to fig. 1, outer tube 51 Lower end be equipped with the threaded joints 511 that are threadedly coupled with the second casing 2.Outer tube 51 and the second casing 2 use thread seal Connection, it is ensured that blowing operation is gone on smoothly to realize the purpose for increasing the air pressure in the second casing 2.

As a kind of specific embodiment of protein desalter provided by the utility model, refering to fig. 1, the second casing 2 upper end is equipped with the first outer 22, and the upper end of threaded joints 511 is equipped with the second outer 512, the first outer 22 and the second outer Sealing element is equipped between 512.The effect of sealing element is the leakproofness improved between outer tube 51 and the second casing 2.

As a kind of specific embodiment of protein desalter provided by the utility model, referring to Fig.2, the first outer The lower surface of 22 upper surface and the second outer 512 is equipped with identical annular groove 6, is equipped with sealing ring 7 between two annular grooves 6. Leakproofness between outer tube 51 and the second casing 2 can effectively improve using sealing ring 7, while easy for installation.

As a kind of specific embodiment of protein desalter provided by the utility model, refering to fig. 1, it is enriched with component 4 include the isolation sieve plate 41 being arranged in the second casing 2 and the filler 42 being arranged in above isolation sieve plate 41.Sieve plate 41 is isolated To play the role of stopping protein, salts substances is allowed to pass through, the effect of filler 42 is the substances such as abundant adhesion protein, peptide fragment, Salts substances are sufficiently disengaged from, increases and is detached from efficiency.The of isolation sieve plate 41 and filler 42 will be installed first in operation Two casings 2 are tightly connected with air blowing component 5, after sample to be purified is added into the second casing 2, realize egg by air blowing component 5 The desalination of white matter.

As a kind of specific embodiment of protein desalter provided by the utility model, filler 42 is C18 key Close one of silicone filler agent, C4 bonded silica gel filler or HILIC filler.These types of filler can be with albumen, peptide fragment Absorption well is generated, sieves salts substances in pumping process, according to the amount of the albumen or peptide fragment that are purified come appropriate Addition filler 42.

As a kind of specific embodiment of protein desalter provided by the utility model, the aperture of sieve plate 41 is isolated It is 20-25 microns, isolation sieve plate 41 can prevent filler from leaking out in waste liquid storage space 3.

As a kind of specific embodiment of protein desalter provided by the utility model, refering to fig. 1, sieve plate is isolated 41 and second casing 2 be interference fitted, thus allow be isolated sieve plate 41 be firmly secured on the second casing 2.

As a kind of specific embodiment of protein desalter provided by the utility model, refering to fig. 1, first sleeve 1 bottom is equipped with the second through-hole 11, and the second through-hole 11 is equipped with sealing-plug 8.After desalination experiment, openable sealing-plug 8 Waste liquid is taken out.

The above is only the preferred embodiments of the present utility model only, is not intended to limit the utility model, all practical at this Made any modifications, equivalent replacements, and improvements etc., should be included in the guarantor of the utility model within novel spirit and principle Within the scope of shield.

Claims

1. protein desalter characterized by comprising

First sleeve；

Second casing, non-tight are arranged in the first sleeve, the bottom of second casing and the bottom of the first sleeve Waste liquid storage space is equipped between portion, the bottom of second casing is equipped with first through hole；

2. protein desalter as described in claim 1, which is characterized in that the air blowing component includes and described second set The outer tube that the upper end of pipe is tightly connected and the piston rod being arranged in the outer tube.

3. protein desalter as claimed in claim 2, which is characterized in that the lower end of the outer tube is equipped with and described the The threaded joints of two casing threads connection.

4. protein desalter as claimed in claim 3, which is characterized in that the upper end of second casing is equipped with outside first The upper end on edge, the threaded joints is equipped with the second outer, and sealing element is equipped between first outer and second outer.

5. protein desalter as claimed in claim 4, which is characterized in that the upper surface of first outer and described The lower surface of two outers is equipped with identical annular groove, is equipped with sealing ring between two annular grooves.

6. protein desalter as described in claim 1, which is characterized in that the enrichment component includes setting described the Isolation sieve plate in two casings and the filler being arranged in above the isolation sieve plate.

7. protein desalter as claimed in claim 6, which is characterized in that the filler is the filling of C18 bonded silica gel One of agent, C4 bonded silica gel filler or HILIC filler.

8. protein desalter as claimed in claim 6, which is characterized in that the aperture of the isolation sieve plate is that 20-25 is micro- Rice.

9. protein desalter as claimed in claim 6, which is characterized in that the isolation sieve plate and the second casing mistake It is full of cooperation.

10. such as the described in any item protein desalters of claim 1-9, which is characterized in that the bottom of the first sleeve Equipped with the second through-hole, second through-hole is equipped with sealing-plug.