CN209162094U - Production-scale stainless steel digester device and biological culture system - Google Patents
Production-scale stainless steel digester device and biological culture system Download PDFInfo
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- CN209162094U CN209162094U CN201821939547.8U CN201821939547U CN209162094U CN 209162094 U CN209162094 U CN 209162094U CN 201821939547 U CN201821939547 U CN 201821939547U CN 209162094 U CN209162094 U CN 209162094U
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- stainless steel
- digester
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- tank
- steel digester
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Abstract
The utility model discloses a kind of production-scale stainless steel digester device and biological culture systems.Production-scale stainless steel digester device includes movable supporting frame and the stainless steel digester being mounted on movable supporting frame, movable supporting frame includes pedestal, bracket and at least two support frames being fixedly connected on bracket, and the bottom of pedestal is provided with roll wheel assembly；Stainless steel digester includes digester and top cover, digester includes tank body, mixing component and the tank bottom for being fixed at tank base, discharge port is provided with below tank bottom, and sieve plate or strainer are additionally provided in tank bottom, liquid collecting cavity is formed between sieve plate or strainer and bottom surface, it is additionally provided with collet in the outer surface of tank body, collet is also connect with steam generation and recirculation assembly.Production-scale stainless steel digester device provided by the embodiment of the utility model can move, convenient for operations such as cell dissociation inoculation, carrier recovery, sterilizing, harvest expression product, full volumetric culture fluid exchanges.
Description
Technical field
In particular to a kind of production-scale stainless steel digester device of the utility model and biological culture system, belong to life
Object culture device technical field.
Background technique
Zooblast for producing protein medicaments, vaccine, small-molecule drug needs to be attached to anchorage dependence
It could be grown on carrier.During large-scale industrial production, needs to separate cell with microcarrier digestion, after cell enrichment, turn
It moves on new microcarrier, realize the amplification of cell and amplifies step by step.Cell with microcarrier digestion separation process be realize rule
The key step of modelling amplification.
The digester of market sale mainly includes the glass digester of small pilot-scale.According to 5g/L microcarrier, microcarrier
Expansion factor is that 20ml/g is calculated, and the liquid amount of 500L scale reaction device is 350L, and microcarrier dead volume when digestion is 35L,
The volume of digester is necessary for 50L (calculating according to liquid amount 70%), is difficult to mechanism for the glass digester of 40L or more scale
Or blow molding.Therefore, the digester of pilot scale and production mostly uses stainless steel digester.The existing stainless steel digester in market is deposited
In three problems: (1) digester and bioreactor put distance far, the liquid relief pipeline for needing connection long between the two, in pipeline
Remaining microcarrier and cell cause the waste of product；(2) stainless steel digester is sterilized using sterilizing cabinet or autoclave, and sterilizing is difficult to
Thoroughly, microbiological contamination situation happens occasionally；(3) ratio of height to diameter of the ratio of height to diameter of digester according to bioreactor, the intracorporal carrier heap of tank
Product, frequently results in liquid relief line clogging and carrier cell mixing is uneven.
Utility model content
The main purpose of the utility model is to provide a kind of production-scale stainless steel digester device and biological cultures
System, with overcome the deficiencies in the prior art.
For the aforementioned purpose of utility model of realization, the technical solution adopted in the utility model includes:
The utility model embodiment provides a kind of production-scale stainless steel digester device comprising movable supporting frame
And it is mounted on the stainless steel digester on movable supporting frame,
The movable supporting frame includes the pedestal being arranged along first direction, the bracket being arranged in a second direction and fixed company
At least two support frames on bracket are connect, the pedestal is fixedly connected with bracket, and the bottom of the pedestal is provided with rolling
Wheel assembly, support frame as described above can be connect and at least described the stainless steel digester to be fixed on stainless steel digester
On movable supporting frame,
The stainless steel digester includes digester and the openable top cover being arranged in digester tank mouth, the digester
Including tank body, mixing component and the tank bottom for being fixed at tank base, discharge port is provided with below tank bottom, and in institute
It states and is additionally provided with sieve plate or strainer in tank bottom, form liquid collecting cavity, the sieve between the sieve plate or strainer and bottom surface
Plate or strainer merge with tank body matching at least to retain microcarrier, and the mixing component is arranged above sieve plate or strainer；
And be additionally provided with collet in the outer surface of the tank body, the collet also with steam pipework or water supply pipe or cooling medium
Piping connection.
In some more specific embodiments, the aperture of the sieve plate or strainer is at least sequentially reduced by upper.
In some more specific embodiments, at least two lifting lugs are further fixedly arranged on outside the tank body, in institute
The fixing groove for being additionally provided on support frame and matching with the lifting lug is stated, the lifting lug can be arranged in the fixing groove.
In some more specific embodiments, the first connecting tube is provided with above the collet, in the folder
Second connecting tube of the lower section setting of set, first connecting tube and the second connecting tube and steam pipework or water supply pipe or cooling
Medium pipeline connection, at least one flow deflector is arranged in the collet, at least forms spiral helicine channel in the collet.
In some more specific embodiments, a plurality of flow deflectors are provided in the collet, the flow deflector is
Arc-like sheet.
In some more specific embodiments, the sieve plate or strainer be set the threaded hole in its four peripheral part with
And threaded fasteners are connected to the peripheral part of tank bottom.
In some more specific embodiments, the shape of the tank bottom is recessed spherical shape, ellipse, dish or cone
Shape.
In some more specific embodiments, the top cover is spirally connected through top cover fastener with tank body, in the tank body
On be additionally provided with the visor that can observe tank interior, be additionally provided on top cover more than one spare mouth, crosspointer material-feeding port,
Temperature electrode mouth, liquid relief mouth, blow vent and sample tap；The mixing component includes servo motor and the servo motor transmission
The agitating shaft of connection and the agitating paddle being fixed on agitating shaft, the agitating paddle are arranged above sieve plate or strainer, institute
The inclination angle for stating stirrer paddle is 5.5-24.5 °, and the ratio between diameter and tank body internal diameter of agitating paddle are 1.0:1.35~1.0:1.95,
The revolving speed of stirrer paddle is 20-200rpm.
In some more specific embodiments, the digester is column structure, and the ratio of height to diameter of the digester is
0.5-1.5:1, preferably tank body ratio of height to diameter≤1.0:1.
In some more specific embodiments, support frame as described above is triangle support frame.
The utility model embodiment additionally provides a kind of biological culture system comprising bioreactor and the life
The stainless steel digester device of production scale, the stainless steel digester device and bioreactor are connected through liquid relief pipeline, and institute
It is adjustable to state the distance between stainless steel digester device and bioreactor.
Compared with prior art, include: the advantages of the utility model
(1) production-scale stainless steel digester device provided by the embodiment of the utility model, structure is simple, and can be with
Transfer movement, convenient for operations such as cell dissociation inoculation, carrier recovery, sterilizing, harvest expression product, all culture fluid exchanges；
(2) using online steam sterilizing in situ, avoid that tank body is huge to cause tank body to be difficult to sterilize and thoroughly happen；
(3) digester ratio of height to diameter is designed using the ratio of height to diameter of 0.5-1.5:1, reduces carrier piling height, is conducive to carrier
Mixing with cells is uniform and the transmission of liquid, shortens the digestion reaction time.
Detailed description of the invention
Fig. 1 is that a kind of structure of production-scale stainless steel digester device is shown in one exemplary embodiments of the utility model
It is intended to；
Fig. 2 is the schematic diagram of the section structure of stainless steel digester in one exemplary embodiments of the utility model；
Fig. 3 is the structural schematic diagram of digester in one exemplary embodiments of the utility model；
Fig. 4 is the structural schematic diagram of the top cover of digester in one exemplary embodiments of the utility model.
Specific embodiment
In view of deficiency in the prior art, inventor is studied for a long period of time and is largely practiced, and is able to propose that this is practical new
The technical solution of type.The technical solution, its implementation process and principle etc. will be further explained as follows.
The utility model embodiment provides the utility model embodiment and provides a kind of production-scale stainless steel digestion
Device device comprising movable supporting frame and the stainless steel digester being mounted on movable supporting frame,
The movable supporting frame includes the pedestal being arranged along first direction, the bracket being arranged in a second direction and fixed company
At least two support frames on bracket are connect, the pedestal is fixedly connected with bracket, and the bottom of the pedestal is provided with rolling
Wheel assembly, support frame as described above can be connect and at least described the stainless steel digester to be fixed on stainless steel digester
On movable supporting frame,
The stainless steel digester includes digester and the openable top cover being arranged in digester tank mouth, the digester
Including tank body, mixing component and the tank bottom for being fixed at tank base, discharge port is provided with below tank bottom, and in institute
It states and is additionally provided with sieve plate or strainer in tank bottom, form liquid collecting cavity, the sieve between the sieve plate or strainer and bottom surface
Plate or strainer merge with tank body matching at least to retain microcarrier, and the mixing component is arranged above sieve plate or strainer；
And be additionally provided with collet in the outer surface of the tank body, the collet also with steam pipework or water supply pipe or cooling medium
Piping connection.
In some more specific embodiments, the aperture of the sieve plate or strainer is at least sequentially reduced by upper.
In some more specific embodiments, at least two lifting lugs are further fixedly arranged on outside the tank body, in institute
The fixing groove for being additionally provided on support frame and matching with the lifting lug is stated, the lifting lug can be arranged in the fixing groove.
In some more specific embodiments, the first connecting tube is provided with above the collet, in the folder
Second connecting tube of the lower section setting of set, first connecting tube and the second connecting tube and steam pipework or water supply pipe or cooling
Medium pipeline connection, at least one flow deflector is arranged in the collet, at least forms spiral helicine channel in the collet.
In some more specific embodiments, a plurality of flow deflectors are provided in the collet, the flow deflector is
Arc-like sheet.
In some more specific embodiments, the sieve plate or strainer be set the threaded hole in its four peripheral part with
And threaded fasteners are connected to the peripheral part of tank bottom.
In some more specific embodiments, the shape of the tank bottom is recessed spherical shape, ellipse, dish or cone
Shape.
In some more specific embodiments, pedestal can be horizontally disposed with, bracket and the angled setting of pedestal, so that
Bracket can be used as baffle or push and pull bar, and the angle between first direction and second direction can be 90 ° or other obtuse angle angles
Degree；And pedestal and bracket may be a fixed connection or pedestal and bracket are flexibly connected, so that the angle between pedestal and bracket
Spend adjustable, such as pedestal connects with bracket through a horizontal rotating shaft, and bracket can be axis in certain angular range using horizontal rotating shaft
Interior rotation；In some more specific embodiments, also there is lower concave part corresponding with the tank bottom of digester on pedestal, under
Recess portion size is greater than the size of tank bottom, and shape is identical as the shape of tank bottom, such as the shape of lower concave part can be recessed ball
Shape, ellipse, dish or taper.
In some more specific embodiments, the top cover is spirally connected through top cover fastener with tank body, in the tank body
On be additionally provided with the visor that can observe tank interior, be additionally provided on top cover more than one spare mouth, crosspointer material-feeding port,
Temperature electrode mouth, liquid relief mouth, blow vent and sample tap；The mixing component includes servo motor and the servo motor transmission
The agitating shaft of connection and the agitating paddle being fixed on agitating shaft, the agitating paddle are arranged above sieve plate or strainer, institute
The inclination angle for stating stirrer paddle is 5.5-24.5 °, and the ratio between diameter and tank body internal diameter of agitating paddle are 1.0:1.35~1.0:1.95,
The revolving speed of stirrer paddle is 20-200rpm.
In some more specific embodiments, the digester is column structure, and the ratio of height to diameter of the digester is
0.5-1.5:1, preferably tank body ratio of height to diameter≤1.0:1.
In some more specific embodiments, support frame as described above is triangle support frame.
The utility model embodiment additionally provides a kind of biological culture system comprising bioreactor and the life
The stainless steel digester device of production scale, the stainless steel digester device and bioreactor are connected through liquid relief pipeline, and institute
It is adjustable to state the distance between stainless steel digester device and bioreactor.
The technical solution, its implementation process and principle etc. will be further explained in conjunction with attached drawing as follows.
Please refer to Fig. 1-Fig. 3, a kind of production-scale stainless steel digester device comprising movable supporting frame 1 and peace
Stainless steel digester 2 on movable supporting frame,
Movable supporting frame 1 includes the pedestal 12 being arranged along first direction, the bracket 11 being arranged in a second direction and fixes
It is connected to the support frame 14 of two triangles on bracket 11, pedestal 12 is vertical with bracket 11 to be fixedly connected, and in pedestal 12
Bottom is provided with roll wheel assembly 13, and support frame 14 can connect with stainless steel digester 2 and at least to by stainless steel digester
It is fixed on movable supporting frame 1,
Stainless steel digester 2 includes digester 21 and the openable top cover 22 being arranged in digester tank mouth, and top cover 22 passes through
Top cover fastener (such as fixing bolt) 7 is spirally connected with tank body；Digester is column structure, and the ratio of height to diameter of digester is 0.5-1.5:
1；Digester includes tank body 21, mixing component and the tank bottom 23 for being fixed at 21 bottom of tank body, is arranged below tank bottom 23
There is discharge port 24, is additionally provided with collet (because angle problem is being not shown in the figure) in the outer surface of tank body 21, is set above collet
Be equipped with the first connecting tube 25, be provided with the second connecting tube 26 below collet, collet through the first connecting tube 25, the second connecting tube 26 with
Steam generation and recirculation assembly, which connect and form steam sterilizing system, (or forms cooling with water supply pipe or cooling medium pipeline
System), a plurality of arc flow deflectors are provided in collet, and then spiral helicine steam (water or cooling Jie are formed in collet
Matter) channel (not shown)；It is additionally provided in the outside of tank body and is able to observe that the visor 28 of tank interior and symmetrical solid
There are two trapezoidal lifting lug 6, lifting lug 6 is ridden upon in the fixing groove on support frame 14 for fixed setting；23 end socket of tank bottom is spherical, oval
Four peripheral parts of shape, dish, taper, four peripheral parts of tank bottom 23 and sieve plate or strainer 27 through threaded hole 271, threaded hole 231 with
And threaded connector (such as screw) is fixedly connected, and then forms liquid collecting cavity between sieve plate or strainer 27 and 23 surface of tank bottom
Body, sieve plate or strainer 27 merge at least with tank body matching to retain microcarrier；It is also equipped in four peripheral parts of tank bottom 23
Annular groove 232；Mixing component therein may include servo motor 3, the electric machine support connecting with servo motor 4 and servo
The agitating shaft that motor 3 is sequentially connected and the agitating paddle 31 being fixed on agitating shaft, agitating paddle 31 is arranged in tank body 21, and stirs
Mix the top that paddle is located at sieve plate or strainer 27；Magnet fluid sealing body component 5 is additionally provided between mixing component and top cover；It please join
Fig. 4 is read, also set up on top cover 22 there are two spare mouth 221, crosspointer material-feeding port 222, temperature electrode mouth 223, liquid relief mouth 224, led to
Port 225 and sample tap 226.Above only to the main of stainless steel digester device production-scale in the utility model embodiment
Structure is illustrated, other accessory assembly therefors can use structure well known by persons skilled in the art, the shape and tool of each component
Body type of attachment repeats no more.
Compared with prior art, include: the advantages of the utility model
(1) production-scale stainless steel digester device provided by the embodiment of the utility model, structure is simple, and can be with
Transfer movement, convenient for operations such as cell dissociation inoculation, carrier recovery, sterilizing, harvest expression product, all culture fluid exchanges；
(2) using online steam sterilizing in situ, avoid that tank body is huge to cause tank body to be difficult to sterilize and thoroughly happen；
(3) digester ratio of height to diameter is designed using the ratio of height to diameter of 0.5-1.5:1, reduces carrier piling height, is conducive to carrier
Mixing with cells is uniform and the transmission of liquid, shortens the digestion reaction time.
It should be appreciated that above-described embodiment is only to illustrate the technical ideas and features of the present invention, it is ripe its object is to allow
The personage for knowing technique can understand the content of the utility model and implement accordingly, can not limit the utility model with this
Protection scope.All equivalent change or modifications according to made by the spirit of the present invention essence, should all cover in the utility model
Within protection scope.
Claims (10)
1. a kind of production-scale stainless steel digester device, it is characterised in that including movable supporting frame and be mounted on removable
Stainless steel digester on bracket,
The movable supporting frame includes the pedestal being arranged along first direction, the bracket being arranged in a second direction and is fixedly connected on
At least two support frames on bracket, the pedestal are fixedly connected with bracket, and the bottom of the pedestal is provided with roller group
Part, support frame as described above can be connect and at least described removable the stainless steel digester to be fixed on stainless steel digester
On dynamic bracket,
The stainless steel digester includes digester and the openable top cover being arranged in digester tank mouth, and the digester includes
Tank body, mixing component and the tank bottom for being fixed at tank base are provided with discharge port below tank bottom, and in the tank
Sieve plate or strainer are additionally provided on bottom, between the sieve plate or strainer and bottom surface formed liquid collecting cavity, the sieve plate or
Strainer merges with tank body matching at least to retain microcarrier, and the mixing component is arranged above sieve plate or strainer；And
It is additionally provided with collet in the outer surface of the tank body, the collet also connects with steam pipework or water supply pipe or cooling medium pipeline
It connects.
2. production-scale stainless steel digester device according to claim 1, it is characterised in that: outside the tank body also
At least two lifting lugs are fixedly installed, the fixing groove to match with the lifting lug, the lifting lug are additionally provided on support frame as described above
It can be arranged in the fixing groove.
3. production-scale stainless steel digester device according to claim 1, it is characterised in that: in the upper of the collet
Side is provided with the first connecting tube, in the second connecting tube that the lower section of the collet is arranged, first connecting tube and the second connection
Pipe and steam pipework or water supply pipe or cooling medium piping connection, are arranged at least one flow deflector, at least in the collet
Spiral helicine channel is formed in the collet.
4. production-scale stainless steel digester device according to claim 3, it is characterised in that: setting in the collet
There are a plurality of flow deflectors, the flow deflector is arc-like sheet.
5. production-scale stainless steel digester device according to claim 1, it is characterised in that: the sieve plate or strainer
It is set the peripheral part that threaded hole and threaded fasteners in its four peripheral part are connected to tank bottom.
6. production-scale stainless steel digester device according to claim 1 or 5, it is characterised in that: the tank bottom
Shape is recessed spherical shape, ellipse, dish or taper.
7. production-scale stainless steel digester device according to claim 1, it is characterised in that: the top cover is through top cover
Fastener is spirally connected with tank body, and the visor that can observe tank interior is additionally provided on the tank body, is additionally provided on top cover
More than one spare mouth, crosspointer material-feeding port, temperature electrode mouth, liquid relief mouth, blow vent and sample tap；The mixing component includes
Servo motor, the agitating shaft being connect with the servo motor transmission and the agitating paddle being fixed on agitating shaft, it is described to stir
Paddle is mixed to be arranged above sieve plate or strainer.
8. production-scale stainless steel digester device according to claim 1, it is characterised in that: the digester is column
Shape structure, the ratio of height to diameter of the digester are 0.5-1.5:1.
9. production-scale stainless steel digester device according to claim 1, it is characterised in that: support frame as described above three
Angular support frame.
10. a kind of biological culture system, it is characterised in that including bioreactor and as described in any one of claim 1-9
Production-scale stainless steel digester device, the stainless steel digester device and bioreactor connect through liquid relief pipeline,
And the stainless steel digester device and the distance between bioreactor are adjustable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821939547.8U CN209162094U (en) | 2018-11-23 | 2018-11-23 | Production-scale stainless steel digester device and biological culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821939547.8U CN209162094U (en) | 2018-11-23 | 2018-11-23 | Production-scale stainless steel digester device and biological culture system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209162094U true CN209162094U (en) | 2019-07-26 |
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ID=67339856
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821939547.8U Active CN209162094U (en) | 2018-11-23 | 2018-11-23 | Production-scale stainless steel digester device and biological culture system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209162094U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112352966A (en) * | 2020-09-29 | 2021-02-12 | 凯之迪(重庆)医疗科技有限公司 | Biological agitator tank |
-
2018
- 2018-11-23 CN CN201821939547.8U patent/CN209162094U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112352966A (en) * | 2020-09-29 | 2021-02-12 | 凯之迪(重庆)医疗科技有限公司 | Biological agitator tank |
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Effective date of registration: 20221028 Address after: 226200 No.500, Linyang Road, Qidong Economic Development Zone, Nantong City, Jiangsu Province Patentee after: Wotisi (Nantong) Biotechnology Co.,Ltd. Address before: 215000 No.81 Suli Road, Wuzhong District, Suzhou City, Jiangsu Province Patentee before: SUZHOU DIOUYI BIOTECHNOLOGY CO.,LTD. |