CN209102726U - Placenta growth factor fluorescence immune chromatography test paper bar, test board and kit - Google Patents
Placenta growth factor fluorescence immune chromatography test paper bar, test board and kit Download PDFInfo
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- CN209102726U CN209102726U CN201821713233.6U CN201821713233U CN209102726U CN 209102726 U CN209102726 U CN 209102726U CN 201821713233 U CN201821713233 U CN 201821713233U CN 209102726 U CN209102726 U CN 209102726U
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Abstract
The utility model discloses a kind of placenta growth factor fluorescence immune chromatography test paper bar, test board and kit, which is mainly made of sample pad, detection film and water absorption pad.It is coated with PLGF fluorescence antibody in sample pad, detects and is provided with detection line and nature controlling line on film, be coated respectively with PLGF monoclonal antibody and sheep anti-mouse igg polyclonal antibody.Above-mentioned test strips are packaged in the test board being made of upper casing and lower casing, and upper casing is provided with well and observation window, corresponding with the sample pad of test strips and the detection line of detection film, nature controlling line respectively.It include above-mentioned test board, sample cell and the radio-frequency identification card for storing the information such as standard curve in kit.Quick, convenient, accurate, reliable detection to placenta growth factor in measuring samples can be realized using the kit combination fluorescence immunity analyzer, without pre-processing to measuring samples.
Description
Technical field
The utility model relates to the fluorescence immune chromatography detection field of biotechnology, in particular to a kind of placenta growth factor
Fluorescence immune chromatography test paper bar, test board and kit.
Background technique
Placenta growth factor (placenta growth factor, abbreviation PLGF) is mainly closed by syncytiotrophoblast
At can be one to trophocyte's function in conjunction with the tyrosinase receptor for being located at trophocyte and vascular endothelial cell
There is Autocrine and has the albumen of paracrine action to angiogenic growth.PLGF has solely trophocyte and inner skin cell function
Special adjustment effect, can promote new vessels to generate.
PLGF is a kind of marker of high degree of specificity, and PLGF level is aobvious when placenta syncytiotrophoblast has oxygen supply pressure
Writing reduces, and can be used to assess placental insufficiency by the PLGF in detection blood, can also be to eclampsia caused by thus before
Phase predicted, is identified and Treatment monitoring.
Currently, the rapid detection method of placenta growth factor mainly has enzyme-linked immunization, using micro-fluidic fluorescence immunoassay
Method etc., the above method requires to pre-process blood sample to be checked, such as complicated operation for enzyme-linked immunization, needs
The equipment such as specific specialized place and constant temperature, oscillation are wanted, and detects and takes a long time, measurement result is also easy by operator
Qualification influence；It needs to carry out centrifugal treating to blood sample to be checked first using micro-fluidic fluorescent immune method, then
Serum is detected, the required time is longer；The component part of immune chromatography test paper, such as sample pad, have PLGF
Stronger non-specific adsorption causes the PLGF amount for entering detection film to reduce, keeps detected value relatively low, seriously affect diagnostic result.
It needs to pre-process measuring samples in conclusion the detection method and tool of existing placenta growth factor exist
It is time-consuming and laborious, the problem of measured value more relatively low than actual value influence diagnostic result.It can be to placenta growth therefore, it is necessary to design one kind
The factor carries out quick, convenient, accurate, reliable test strips, test board and kit.
Utility model content
The purpose of the utility model is to provide a kind of placenta growth factor fluorescence immune chromatography test paper bar, test board and
Kit, in conjunction with fluorescence immunity analyzer realize in measuring samples placenta growth factor it is quick, convenient, accurate, reliable
Detection.
In order to solve the above-mentioned technical problem, the utility model provides a kind of placenta growth factor fluorescence immune chromatography test paper
Item, the placenta growth factor fluorescence immune chromatography test paper bar include sample pad, detection film and water absorption pad.
The sample pad includes glass fibre membrane.
The detection film includes nitrocellulose filter, cellulose acetate film.Further, detection film is nitrocellulose
Film.
The water absorption pad includes blotting paper.
The glass fibre, nitrocellulose filter, cellulose acetate film and blotting paper are commercially available commodity.
The sample pad, detection film and water absorption pad sequentially overlap.As shown in Figure 1 and Figure 2, sample pad and detection film
One end overlap joint, the other end and water absorption pad for detecting film overlap.Sample pad that mutually overlapped and detection film, detection film and water absorption pad
Overlying relation can be interchanged, and be not limited to attached overlying relation shown in Fig. 2.
The region contacted between the sample pad and detection film, detection film and water absorption pad can be auxiliarily fixed without using any
Articles, such as sample pad, detection film and water absorption pad are put into card slot and keep its specific positional relationship.Also bonding can be used
Mode keep its specific positional relationship.
The outer dimension of the sample pad, detection film and water absorption pad is routine, is limited with can be realized measurement target.
The sample pad the preparation method is as follows: step 1) impregnate: sample pad is put into the solution of sealant compositions
It impregnates 0.5 hour~2.0 hours；Step 2) is dry: the sample pad of step 1) taken out, it is 3 hours dry in 20 DEG C~40 DEG C
~7 hours.
Sealant compositions used in the closing sample pad include Emulsifier EL-60, polyvinyl alcohol, trehalose, sugarcane
Sugar and buffer, the mass concentration percentage of Emulsifier EL-60, polyvinyl alcohol, trehalose and sucrose in the buffer
For (0.25%~0.55%): (0.05%~0.30%): (1.0%~6%): (10%~20%)；
The buffer includes phosphate buffer, and phosphate concentration is 40mmol/L to 100mmol/L, and pH value is
6.0 to 8.0.
Sample pad using above-mentioned enclosure method preparation has lower non-specific adsorption effect, in sample to be examined
Target substance absorption is few, ensure that the combination of target substance and fluorescence antibody, improves the accuracy of detection；Make sample pad with it is glimmering
Adsorption between photoactivated antibody is in reasonable interval, improves the desorption efficiency of fluorescence antibody in immune chromatography test paper use, drop
Low detection limit, improves detection accuracy；Immune chromatography test paper is set to be suitable for the detection to whole blood sample, without to whole blood sample
It is pre-processed, simplifies detection process.
PLGF fluorescence antibody is coated in the sample pad, alternatively referred to as " the PLGF monoclonal of coupling fluorescent microsphere is anti-
Body ".
The coating weight of PLGF fluorescence antibody in the sample pad is 0.1 μ of μ g~2 g.
The PLGF fluorescence antibody, PLGF monoclonal antibody and fluorescent microsphere are commercial antibodies available on the market and glimmering
Luminescent material.
The preparation method of the PLGF fluorescence antibody is prepared using this field routine techniques, the specific steps are as follows: first with saturating
Sodium azide in the method removal antibody reagent of analysis；Then with 2- (N- morpholino) ethanesulfonic acid of the pH6.0 of concentration 50mM
(MES) antibody is diluted to 1mg/mL by buffer；Microballoon is added to 2- (N- morpholino) ethanesulfonic acid of the pH6.0 of concentration 50mM
(MES) in buffer, sonic oscillation 10min makes its dispersion；1- (3- dimethylamino-propyl) -3- is added in above-mentioned suspension
Ethyl-carbodiimide hydrochloride (EDC) makes its final concentration of 10mg/mL, is incubated for 15min at 26 DEG C, microballoon is activated；It will be micro-
The centrifugation of ball suspension, removes supernatant, adds 2- (N- morpholino) ethanesulfonic acid of the pH6.0 of isometric concentration 50mM
(MES) buffer, suspended microspheres, are centrifuged and remove supernatant again；Again with the 2- (N- of the pH6.0 of isometric concentration 50mM
Morpholino) ethanesulfonic acid (MES) buffer, suspended microspheres, are added above-mentioned antibody-solutions, 5h are reacted at 26 DEG C, is not during which stopped again
Oscillation or stirring；Ethanol amine, mixing oscillation 15min is added；It will be finally centrifuged containing PLGF fluorescence antibody suspension, in removal
Clear liquid, for use.
PLGF fluorescence antibody described in the utility model is also by closing step.
The closing step include: by the PLGF fluorescence antibody after washing be suspended in the solution of sealant compositions into
Row closing.
Sealant compositions used in the closing PLGF fluorescence antibody include Emulsifier EL-60, polyvinyl alcohol, sea
Algae sugar, sucrose and buffer, the quality of Emulsifier EL-60, polyvinyl alcohol, trehalose and sucrose in the buffer are dense
Spending percentage is (0.02%~0.25%): (0.05%~0.30%): (1.0%~6%): (10%~20%)；
The buffer includes phosphate buffer, and phosphate concentration is 10mmol/L to 40mmol/L, and pH value is
6.0 to 8.0.
There is special property through above-mentioned enclosure method treated fluorescence antibody, fluorescence antibody can be made in test strips system
It is adsorbed in sample pad during standby, and desorbs fluorescence antibody easily from sample pad in using test strips detection process
Get off, in sample liquid or test liquid be moved to detection film, and be moved to corresponding position, have test strips preferably
Detectability.After the enclosure method processing, acting on the non-specific adsorption of fluorescence antibody is reduced, and is reduced to blood
The absorption of other biological macromolecular substances in sample can be such that accuracy in detection and stability improves.
Detection line and nature controlling line are disposed on the detection film, detection line and nature controlling line are parallel to each other, wherein detecting
Line is close to sample pad, and nature controlling line is far from sample pad.Between the distance between the nature controlling line and water absorption pad, detection line and nature controlling line
Distance be this field conventional arrangement.
The detection line is coated with PLGF monoclonal antibody.
The amount for the PLGF monoclonal antibody being coated in the detection line is 0.1 μ of μ g~1.5 g.
The nature controlling line is coated with sheep anti-mouse igg polyclonal antibody.
The sheep anti-mouse igg Anti-TNF-α scale of construction being coated on the nature controlling line is 0.1 μ of μ g~3.0 g
The PLGF monoclonal antibody and sheep anti-mouse igg polyclonal antibody are commercial antibody available on the market.
The preparation process of the detection film is as follows: with the drying process after drawing film, stroke film of continuous state operation, and setting
After drawing film after drying process, with drying process after the film closing of continuous state operation, closing；Described stroke of film is done after drawing film
The runing time of drying process is 2~4min, and the runing time of drying process is 3~6min after the film closing, closing.
The step of preparation process of the detection film, is as follows:
A, dry after drawing film, drawing film:
A1, it draws film: NC film roll being taken to be placed in out on reel, then by drawing film instrument, the antibody of required concentration will be diluted to
Solution is crossed on NC film or sprays line；
A2, draw drying after film: while NC film rear end is crossed, the NC film front end that scribing line is completed enters at once to be mentioned
Before be preheated in the drying tower of drying temperature, complete drying；
B, drying process after film closing, closing
B1, film closing: by the NC film after step a2 is dry, at the uniform velocity by the immersion liquid slot added with confining liquid, to NC film into
Row closing；
The confining liquid is mainly dissolved in the PB of pH7.0~8.0,50~60mM by closing component, surface active agent composition
It is made in solution；The closed group point includes at least one of PEG4000, bovine serum albumin, gelatin, formaldehyde, casein, institute
Stating mass percentage of the closing component in confining liquid is 0.5~1.0%；The surface active agent composition includes tween-
20, Pluranic L64, Cremophor EL, Surfynol 485, Tetronic 1307, in TRITON X-100 at least
One kind, mass percentage of the surface active agent composition in confining liquid are 0.5~1.0%；
It is dry after b2, closing: while NC film rear end is closed, closed NC film front end is completed to enter at once and mention
Before be preheated in the drying tower of drying temperature, complete it is dry after wind.
Drying temperature described in step a2, b2 is 35~40 DEG C.
The confining liquid is mainly dissolved in pH by closing component PEG4000, surface active agent composition Surfynol 485
7.0, in the PB solution of 50mM and adjust confining liquid pH to 7.0 be made；Described PEG4000, Surfynol 485 is in confining liquid
Mass percentage be respectively 0.5%, 0.6%.
Surface active agent composition in the confining liquid further includes Pluranic L64, and the Pluranic L64 is being closed
Mass percentage in liquid is 0.2%.
Using the above-mentioned detection film with highly sensitive, low detection limit characteristic, it is possible to prevente effectively from because in blood sample
PLGF concentration is lower and the case where showing that detected value is zero, correct medical judgment can not be made, influence diagnosing and treating；It can also
Effectively to avoid the diagnostic result of false positive (for example, PLGF actual concentrations are about 80 pg/mL, other test boards in blood sample
Detected value be shown as 110pg/mL, the diagnostic criteria higher than >=100pg/mL, and be diagnosed as the situation of normal feminine gender), make
At mistaken diagnosis, delay the case where treating Best Times.
The non-specific adsorption to test substance can be reduced using the sample pad that above-mentioned sample pad enclosure method obtains.It adopts
The absorption to interfering substance can be reduced with the fluorescence antibody that above-mentioned fluorescence antibody enclosure method obtains, and can be with sample pad
Preferable separation, into detection film.There is highly sensitive, low detection limit using the detection film of above-mentioned detection membrane preparation method preparation
Characteristic, can enable to detect film and effectively avoid the interference of other substances, there is higher specificity to the detection of PLGF.It is above-mentioned
The characteristic of sample pad, fluorescence antibody and detection film reaches the minimum detection limit of PLGF fluorescence immune chromatography and is less than 12pg/mL,
In the range of PLGF concentration is 12pg/mL to 3000pg/mL, linearly dependent coefficient r >=0.95, and the accuracy of measurement result
Higher, standard deviation is no more than 8%.
In order to solve the above-mentioned technical problem, the utility model also provides a kind of placenta growth factor fluorescence immune chromatography test
Plate, the placenta growth factor fluorescence immune chromatography test board include getting stuck and being arranged in the interior test strips that get stuck.
It is described to get stuck including upper casing and lower casing, it is provided with well and observation window on upper casing, the well corresponds to test paper
The sample pad of item, the corresponding detection line and nature controlling line detected on film of the observation window.
The card slot of fixed test strips is provided on the upside of the lower casing, shape is identical as test strips therein are placed.
The shape of the well and observation window includes but is not limited to attached shape shown in Fig. 4, such as well can be with
It is ellipse or quadrangle etc., observation window can also be oblong, and quadrangle has the rectangle etc. of radian.
The upper casing to get stuck and lower casing can be spliced using detachable, and internal test strips can be according to need
It replaces, gets stuck reusable；Upper casing and lower casing can also be welded by the way of welding, such as with the mode of ultrasonic welding
It picks up and.
The upper surface of the upper casing is also coated with two dimensional code, recognizes test card to be detected for fluorescence immunity analyzer,
It eliminates and the operation such as is manually entered, improve detection speed, save the operating time, it is ensured that the accuracy of detection and analysis.
The interior test strips that get stuck that are arranged in are placenta growth factor fluorescence immune chromatography test paper bars described previously.
When carrying out placenta growth factor concentration mensuration using the test board, required measuring samples amount is few, such as only
Need 100 μ of μ L~300 L.
In order to solve the above-mentioned technical problem, the utility model also provides a kind of placenta growth factor fluorescence immune chromatography reagent
Box, the placenta growth factor fluorescence immune chromatography kit include placenta growth factor fluorescence immune chromatography test board, sample cell
And radio-frequency identification card.
Multiple test boards and sample cell are housed in the kit, test board and sample cell can also be packed independently,
To avoid unnecessary outside contamination, the Stability and veracity of detection is influenced.
Standard curve is stored in the radio-frequency identification card.
Kit testing principle described in the utility model and process are as follows: the blood sample to be measured of prescribed volume is added to sample
On product pad, the PLGF in blood sample to be measured can form fluorescence antibody-PLGF compound, at this time in conjunction with PLGF fluorescence antibody
There are also the PLGF fluorescence antibodies not in conjunction with PLGF in sample pad.Fluorescence antibody-PLGF compound and not in conjunction with PLGF
PLGF fluorescence antibody is chromatographed to detection line through capillary action, and fixed PLGF monoclonal antibody can be anti-with fluorescence in detection line
Another site combines on the PLGF of body-PLGF compound, forms PLGF fluorescence antibody-PLGF-PLGF monoclonal antibody complex,
The compound is retained in detection line.And the PLGF fluorescence antibody not in conjunction with PLGF is not mono- with PLGF fixed in detection line
Clonal antibody combines, and can cross detection line, and chromatography to nature controlling line is captured by the sheep anti-mouse igg polyclonal antibody on nature controlling line,
Sheep anti-mouse igg polyclonal antibody-PLGF fluorescence antibody compound is formed in nature controlling line.Since PLGF fluorescence antibody is retained in inspection
Survey line and nature controlling line, PLGF concentration is high in blood sample, then the fluorescent material for being integrated to detection line is more；PLGF is dense in blood sample
Spend low, then the fluorescent material for being integrated to nature controlling line is more, it is possible to use the fluorescence in fluorescence immunity analyzer detection two lines
Intensity calculates fluorescence intensity level substitution calibration curve equation, the concentration of PLGF in available blood sample.
Placenta growth factor fluorescence immune chromatography test paper bar, test board and kit provided by the utility model, overcome
It the pretreatment such as needs to incubate measuring samples, be centrifuged in prior art placenta growth factor detection process, make detection time
Extended defect, it is stronger to PLGF non-specific adsorption to also overcome sample pad, makes detected value lower than actual value, seriously affects and examine
The deficiency of disconnected result, can be realized quick, convenient, accurate, the reliable detection to placenta growth factor in measuring samples.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the placenta growth factor fluorescence immune chromatography test paper bar of the utility model；
Fig. 2 is the side schematic view of the placenta growth factor fluorescence immune chromatography test paper bar of the utility model；
Fig. 3 is the placenta growth factor fluorescence immune chromatography test paper bar of the utility model and the opposite position of test board lower casing 7
Set schematic diagram；
Fig. 4 is 6 schematic diagram of test board upper casing of the utility model；
Fig. 5 is the stereoscopic schematic diagram of the placenta growth factor fluorescence immune chromatography test card of the utility model；
Wherein:
1, sample pad, 2, detection film, 3, water absorption pad, 4, detection line, 5, nature controlling line, 6, upper casing, 7, lower casing, 8, well,
9, observation window.
The effect of the utility model
Using the beneficial effect of the placenta growth factor fluorescence immune chromatography test paper bar of the utility model, test board and kit
Fruit is: the detection time of placenta growth factor is 1. greatly shortened, realizes the quick detection of placenta growth factor, so as to the greatest extent
It is early that the critical illness such as preeclampsia are made a definite diagnosis, strive for the time for the effective treatment of associated disease；Exempt from 2. improving and using
The ratio of clinical diagnosis mistake is greatly decreased in the accuracy that epidemic disease chromatography detects PLGF；3. without to blood sample to be checked into
Row pretreatment, simplifies the operating procedure of placenta growth factor detection, saves detection time；4. required sample size is few.
Specific embodiment
Embodiment 1
Using the placenta growth factor fluorescence immune chromatography test board of the utility model shown in attached drawing 5, upper casing 6 and lower casing
7 be to be internally provided with placenta growth factor fluorescence immune chromatography test paper bar as shown in Fig. 1, sample by ultrasonic bonding
Product pad 1 and water absorption pad 3 are placed on lower layer, and detection film 2 is placed on the upside of sample pad 1 and water absorption pad 3, sample pad 1 and detection film
2, detection film 2 overlaps with water absorption pad 3, that is, overlaps, as shown in Fig. 2.Test strips are placed on 7 upside of lower casing
In card slot, as shown in Fig. 3.The sample pad 1 of test strips uses glass fibre membrane, to use sample described in the utility model
The glass fiber sample pad that pad processing method obtains；After the enclosure method processing that PLGF fluorescence antibody is described through the utility model,
It is coated in sample pad 1.The detection film 2 of test strips uses nitrocellulose filter, detects and is provided with detection line 4 and Quality Control on film 2
Line 5 is coated with PLGF monoclonal antibody in detection line 4, sheep anti-mouse igg polyclonal antibody is coated on nature controlling line 5, is then used
The method that the utility model describes carries out Seal treatment.Detection line 4 is arranged in parallel with nature controlling line 5, and is parallel to detection film 2
Short side, wherein detection line 4 is close to sample pad 1, and nature controlling line 5 is far from sample pad 1.Observation window 9 in 6 upside of test board upper casing is long
The side on side is also coated with two dimensional code, stores test board identification relevant information.Above-mentioned test board is placed in kit, wherein
There are also sample cell and radio-frequency identification card, standard curve related data is stored in radio-frequency identification card.
Using the sample cell in kit, the 200 μ L of standard items (100pg/mL) of PLGF standard fixed concentration is drawn, will be inhaled
The standard items taken are all added drop-wise in test board well 8, test board are inserted into fluorescence immunity analyzer, fluoroimmunoassay
Instrument reads the relevant informations such as lot number, fluorescence immunity analyzer adjust automatically location parameter by the two dimensional code on sweep test plate
(such as chromatography temperature, chromatography time, fluorescence exciting wavelength, Detection wavelength), if the standard for storing the lot number in analyzer is bent
Line, then relevant fluorescence intensity on read test plate, and directly the standard can be shown in the display screen of fluorescence immunity analyzer
The concentration of product.If there is no the standard curve of the lot number read in analyzer, the correlation in operator's typing radio-frequency card is prompted
The radio-frequency identification card for storing standard curve is placed at the card reading of fluorescence immunity analyzer, fluorescence immunity analyzer by information
Reading relevant criterion curve, (a batch letter need to be only written in one batch information of a card, the product with batch in analyzer
Batch information can also be first written when first time using the batch kit in breath), typing post analysis instrument continues to scan on test board
Fluorescent value, by fluorescence intensity level substitution calibration curve equation calculate, show the concentration of placenta growth factor.Entire inspection
Survey process is only about 16 minutes time-consuming.
Using with a batch of placenta growth factor fluorescence immune chromatography test board to above-mentioned PLGF standard fixed concentration
Standard items carry out 15 parallel determinations.The ELISA kit that reuse method is mature, stability is good measures same PLGF standard
The standard items of fixed concentration are measured, same to carry out 15 parallel determinations.Calculate the measurement result that two kinds of detection means obtain
Correlation, coefficient R=0.9934 both shows that correlation is good, and the placenta growth factor fluorescence of the utility model is exempted from
The measurement result of epidemic disease chromatography test board can reach the measurement result of mature ELISA kit.But with mature ELISA reagent
Box measuring method is compared, and avoids sample pretreatment using the placenta growth factor fluorescence immune chromatography test board of the utility model
The step of, manual operation is reduced, personnel is avoided and operates bring error, and minute substantially shortens, it is raw to realize placenta
The quick detection of the long factor, the blood sample amount to be checked used are only 200 μ L.
Embodiment 2
Using the placenta growth factor fluorescence immune chromatography test board of the utility model shown in attached drawing 5, upper casing 6 and lower casing
7 be to be connected by a snap, and is internally provided with placenta growth factor fluorescence immune chromatography test paper bar, sample pad 1 and water absorption pad 3
It is placed on lower layer, detection film 2 is placed on the upside of sample pad 1 and water absorption pad 3, sample pad 1, the width for detecting film 2 and water absorption pad 3
Identical, sample pad 1 is overlapped and is bonded together with water absorption pad 3 with detection film 2, detection film 2, that is, is overlapped, such as 2 institute of attached drawing
Show.Test strips are sticked to 7 upside appropriate location of lower casing, which refers to detection line in the sample pad and detection film 1 of test strips
4, nature controlling line 5 is respectively with the well 8 of upper casing 6 and observation window 9 to corresponding.The sample pad 1 of test strips uses glass fibre membrane,
For the glass fiber sample pad for using sample pad processing method described in the utility model to obtain；PLGF fluorescence antibody is practical through this
After the enclosure method processing of novel description, it is coated in sample pad 1.The detection film 2 of test strips uses nitrocellulose filter, detection
It is provided with detection line 4 and nature controlling line 5 on film 2, PLGF monoclonal antibody is coated in detection line 4, is coated with goat-anti on nature controlling line 5
Then mouse IgG polyclonal antibody carries out Seal treatment using the method that the utility model describes.Detection line 4 and nature controlling line 5 are flat
Row setting, and it is parallel to the short side of detection film 2, wherein detection line 4 is close to sample pad 1, and nature controlling line 5 is far from sample pad 1.It is surveying
The side of 9 long side of observation window of 6 upside of test plate (panel) upper casing is also coated with two dimensional code, stores test board identification relevant information.It is above-mentioned
Test board is placed in kit, wherein storing standard curve phase in radio-frequency identification card there are also sample cell and radio-frequency identification card
Close data.
Using the sample cell in kit, 200 μ L of blood sample to be checked is drawn, the sample of absorption is all added drop-wise to test
In plate well 8, test board is inserted into fluorescence immunity analyzer, fluorescence immunity analyzer passes through the two dimension on sweep test plate
Code reads the relevant informations such as lot number, and fluorescence immunity analyzer adjust automatically location parameter is (such as chromatography temperature, chromatography time, fluorescence
Excitation wavelength, Detection wavelength etc.), it is relevant glimmering on read test plate if storing the standard curve of the lot number in analyzer
Luminous intensity, and the concentration of the standard items can be directly shown in the display screen of fluorescence immunity analyzer.If not read in analyzer
The standard curve of the lot number taken then prompts the relevant information in operator's typing radio-frequency card, will store penetrating for standard curve
Frequency identification card is placed at the card reading of fluorescence immunity analyzer, and fluorescence immunity analyzer reads a relevant criterion curve (Zhang Kayi
A batch information need to be only written in a batch information, the product with batch in analyzer, can also use the batch in first time
Batch information is first written when kit), typing post analysis instrument continues to scan on the fluorescent value on test board, and fluorescence intensity level is substituted into
Calibration curve equation is calculated, and shows the concentration of placenta growth factor.Entire detection process is only about 16 minutes time-consuming.To this
Blood sample is measured in parallel 7 times, is calculated the average value of measurement result, is then calculated its relative deviation, as the result is shown measurement result
Relative deviation be 6.3%, show that its is with good stability.
It follows that placenta growth factor fluorescence immune chromatography test board described in the utility model can directly to without from
The step of blood sample of heart processing removal haemocyte is measured, avoids sample pretreatment, reduces manual operation, avoids
Personnel operate bring error, and minute substantially shortens, and realizes the quick detection of placenta growth factor, and use to
Examining blood sample amount is only 200 μ L.
Comparative example 1
Sample pad, fluorescence antibody, detection film are prepared and located using the prior art as known to those skilled in the art
Reason, is then assembled into placenta growth factor fluorescence immune chromatography test board, carries out 7 times in parallel to the blood serum sample that centrifugation obtains
Measurement.The average value for calculating measurement result, then calculates its relative deviation, the relative deviation of measurement result is as the result is shown
14.3%, show that the stability of its measurement is poor.
In conclusion using the placenta growth factor fluorescence immune chromatography test paper bar, test board and reagent of the utility model
Box can obtain the effect essentially identical with the Stability and veracity of ELISA kit method measurement result mature and stable at present
Fruit, and the detection time of placenta growth factor can also be greatly shortened, realize the quick detection of placenta growth factor.Also
Overcome the fluorescence immune chromatography method of the prior art disadvantage inadequate to the PLGF accuracy deficiency detected and stability.It can be direct
Whole blood sample is measured, without pre-processing to blood sample to be checked, simplifies the operation of placenta growth factor detection
Step saves detection time.
Claims (4)
1. a kind of placenta growth factor fluorescence immune chromatography test paper bar, including sample pad (1), detection film (2) and water absorption pad (3),
It is characterized in that, the sample pad (1), detection film (2) and water absorption pad (3) sequentially overlap；
PLGF fluorescence antibody is coated on the sample pad (1)；
The detection film (2) is disposed with detection line (4) and nature controlling line (5), and detection line (4) and nature controlling line (5) are parallel to each other,
Wherein detection line (4) is close to sample pad (1), and nature controlling line (5) is far from sample pad (1)；
The detection line (4) is coated with PLGF monoclonal antibody；
The nature controlling line (5) is coated with sheep anti-mouse igg polyclonal antibody.
2. placenta growth factor fluorescence immune chromatography test paper bar according to claim 1, which is characterized in that the sample pad
It (1) include glass fibre；
The detection film (2) includes nitrocellulose filter, cellulose acetate film；
The water absorption pad (3) includes blotting paper.
3. a kind of placenta growth factor fluorescence immune chromatography test board, which is characterized in that including get stuck and be arranged in get stuck in
Test strips:
It is described to get stuck including upper casing (6) and lower casing (7), well (8) and observation window (9), the sample-adding are provided on upper casing (6)
The sample pad (1) of the corresponding test strips in hole (8), the corresponding detection line (4) and nature controlling line detected on film (2) of the observation window (9)
(5)；
It is described that test strips in getting stuck are set using test strips described in claim 1.
4. a kind of placenta growth factor fluorescence immune chromatography kit, which is characterized in that including placenta growth described in claim 3
Factor fluorescence immune chromatography test board, sample cell and radio-frequency identification card；
Standard curve is stored in the radio-frequency identification card.
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| CN201821713233.6U CN209102726U (en) | 2018-10-22 | 2018-10-22 | Placenta growth factor fluorescence immune chromatography test paper bar, test board and kit |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110763837A (en) * | 2019-11-08 | 2020-02-07 | 河北特温特生物科技发展有限公司 | Electronic quality control card for time-resolved fluorescence immunoassay analyzer and electronic quality control card assembly |
| CN113804899A (en) * | 2021-08-26 | 2021-12-17 | 宁波奥丞生物科技有限公司 | Immunochromatography reagent strip for detecting urine placenta growth factor of pregnant woman and preparation method thereof |
| CN114280312A (en) * | 2020-09-27 | 2022-04-05 | 河北特温特生物科技发展有限公司 | Whole blood separation membrane for immunofluorescence chromatography detection and preparation method and application thereof |
-
2018
- 2018-10-22 CN CN201821713233.6U patent/CN209102726U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110763837A (en) * | 2019-11-08 | 2020-02-07 | 河北特温特生物科技发展有限公司 | Electronic quality control card for time-resolved fluorescence immunoassay analyzer and electronic quality control card assembly |
| CN114280312A (en) * | 2020-09-27 | 2022-04-05 | 河北特温特生物科技发展有限公司 | Whole blood separation membrane for immunofluorescence chromatography detection and preparation method and application thereof |
| CN114280312B (en) * | 2020-09-27 | 2023-09-15 | 河北特温特生物科技发展有限公司 | Whole blood separation membrane for immunofluorescence chromatography detection and preparation method and application thereof |
| CN113804899A (en) * | 2021-08-26 | 2021-12-17 | 宁波奥丞生物科技有限公司 | Immunochromatography reagent strip for detecting urine placenta growth factor of pregnant woman and preparation method thereof |
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