CN106771255A - Immuno-chromatographic test paper strip and detection kit for detecting CRP concentration - Google Patents
Immuno-chromatographic test paper strip and detection kit for detecting CRP concentration Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N2333/4737—C-reactive protein
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Abstract
Immuno-chromatographic test paper strip and detection kit the present invention relates to be used to detect CRP concentration.Specifically, immuno-chromatographic test paper strip of the invention is included on base plate sample pad, coated film, the adsorptive pads that sequentially fixed and end interconnects, and Quality Control coating thing coating area, CRP monoclonal antibody A coatings area and the CRP antigen coats area being spaced from sequentially are distributed with membrane body along the direction from sample pad to adsorptive pads on the coated film.Example reaction liquid of the detection kit of the invention comprising test strips and independent packaging, the example reaction liquid includes the CRP monoclonal antibody B with label, and the material specifically bound with Quality Control coating thing with label.Test strips of the invention under the conditions of the temperature and time of normal operating range, the detection performance with relatively reliable stabilization.
Description
Technical field
The present invention relates to a kind of immuno-chromatographic test paper strip for detecting CRP concentration, and the inspection comprising the test strips
Test agent box.
Background technology
C reactive protein (C-Reactive Protein, CRP) is a kind of classical Acute reaction protein, thin by liver
Born of the same parents synthesize.In Healthy Human Serum CRP concentration it is very low (<5mg/L), the inflammation such as microorganism invasion or tissue damage are subject in body
Concentration is significantly raised during sexual stimulus.Therefore, CRP is one of acute-phase response index the most frequently used in clinical detection.
More the CRP detection methods of early stage are including latex agglutination experimental method, immunoturbidimetry, radioimmunoassay etc.
Deng, but the various defects such as sensitivity is low, detection range is small, isotopic contamination are respectively present, gradually it is eliminated.
There are different demands to sensitivity, specificity when traditionally being assessed due to different physiological situations, in addition detection method
Limitation, super quick detection (detection range of linearity 0.1-10mg/L) is generally divided into the detection of CRP concentration and conventional detection (is examined
Linear scope 3-200mg/L).
In recent years, immunochromatography law technology is developed rapidly, has been developed to be needed while taking into account high concentration and low concentration
Ask, while taking into account the detection method of high sensitivity and the range of linearity wide, be widely used in clinical and laboratory.
Patent application CN105891510A disclose a kind of CRP immunofluorescences chromatography detection coated film, test strips and its
Application method, solving existing CRP immunochromatography techniques can not be while takes into account the wide line range detection and high-sensitivity detection
Problem.The patent application can not dilute CRP detection sample on the premise of, it is ensured that CRP detection simultaneously have concurrently high sensitivity and
The range of linearity wide, it is to avoid traditional detection method needs to carry out clinical sample hundred times dilution to cause sampling deviation.
Patent application CN102023211A disclose a kind of whole quantitative determination c reactive protein immuno-chromatographic test paper strip and
Its preparation method.The test strips quickly can be quantitative determined sensitively to whole CRP, can be detected simultaneously with hs-
CRP and routine two results of CRP, with the comprehensive range of linearity and good detection sensitivity.
The content of the invention
But, existing immunochromatographic method is when human plasma, serum and CRP concentration in whole blood is detected, it is desirable to must be in rule
Accurate result can be just obtained under fixed reaction time, environment temperature and detection time.However, due to by testing staff, doctor
The different limitation such as institute's condition, can simultaneously meet the possibility very little of three above condition.
Sometimes because hospital's testing staff's live load is larger, cannot processing detection trial target in time, or due to detection
Personnel lack training, experience or lack of skill, it is possible to which meeting postpones (1-2min) or carries in the detection time of regulation
Before (1-2min) read measurement result.If reading occurs significant changes in this case, measurement result is easily lead to
Cancel, lose time and money.
In the poor basic hospital of some conditions, sensing chamber is possible to be not provided with heating or air-conditioning.In heat or
Under the extreme weather in person's severe winter, it is impossible to ensure the ordinary temperature of sensing chamber.On the ground of the inclement conditions such as backward areas, chaos caused by war area
Area, this more turns into a kind of normality.Because the reason for these are special, usually can not be in the environment temperature (such as 18 DEG C -30 DEG C) of regulation
Under carry out CRP detection reaction, or even needs detected at a temperature of substantial deviation environment temperature (such as 4 DEG C, 37 DEG C etc.)
Reaction.In addition, existing immunochromatographic method most requirement is stored under refrigerated conditions, but before testing must be by reagent
Recover to room temperature that accurate testing result could be obtained with test strips.If testing staff could not be fully extensive with test strips by reagent
Just detected to room temperature again, it is also possible to cause reading not accurate enough.
Above-mentioned these factors cause that the reaction time of immunochromatographic method, reaction temperature cannot ensure, have a strong impact on detection knot
The accuracy of fruit.
Inventors have surprisingly found that, when immuno-chromatographic test paper strip of the invention is reacted under different environment temperatures
Maintain the accuracy of measurement of stabilization.Inventor have further surprisingly found that, immuno-chromatographic test paper strip of the invention is in different surveys
Fix time when carrying out reading, also maintain good reading stability.
The invention provides a kind of immuno-chromatographic test paper strip for detecting CRP concentration, it is included on base plate and sequentially fixes
And sample pad, coated film, the adsorptive pads that end interconnects, exist along the direction from sample pad to adsorptive pads on the coated film
Quality Control coating thing coating area (C lines), the CRP monoclonal antibody A coatings area (T1 being spaced from sequentially are distributed with membrane body
Line) and CRP antigen coats area (T2 lines).It will be appreciated by those skilled in the art that, area is coated with operation is put into practice often using instrument
Device or manually line are obtained, and the line thickness of its wire outward appearance can be ignored not for the length of whole test strips
Meter.
In test strips of the invention, Quality Control coating thing is selected from anti-rabbit IgG, rabbit igg, biotin, Avidin or is combined with life
Albumen of thing element or Avidin etc., preferably anti-rabbit IgG.
In test strips of the invention, the coating concentration of Quality Control coating thing is 0.1-10mg/mL, preferably 0.6-4mg/mL.
In test strips of the invention, the coating concentration of CRP monoclonal antibody A is 0.5-10mg/mL, preferably 1-4mg/mL.
In test strips of the invention, the coating concentration of CRP antigens is 0.1-10mg/mL, preferably 0.6-2mg/mL.
In test strips of the invention, the spacing distance between Quality Control thing coating area and CRP monoclonal antibody A coatings area is 2-
8mm, preferably 2-4mm.
In test strips of the invention, the spacing distance between CRP monoclonal antibody A coating areas and CRP antigen coats area is
2-8mm, preferably 2-4mm.
Spacing distance in test strips of the present invention between C lines, T1 lines, T2 lines can as needed be come by those skilled in the art
It is determined that.When preferably taking scope as described above, formation intersects so as to avoid and cannot obtain between three lines are avoided first
Take the situation of true detection data, and reactant mixture by regional time also will not difference it is too big, so as to avoid
Isolate whole relation, the situation of final influence testing result between three lines.
" test strips " alleged in context, refer to the tomographic system mode commonly used in immunochromatography technique, it is also possible to
It is the common types such as test card, reagent card, as long as it makes use of immunochromatography technique principle.
In test strips of the invention, base plate can be the various inertia base plates commonly used in immunochromatography technique, preferably polychlorostyrene
Ethene PVC base plates.
In test strips of the invention, fixation can be embodied in various ways using commonly used in the art, as long as various pieces
It is difficult to disengage the mutual state adhered under external force.Fixed form includes but is not limited to heat lamination techniques and fixes, bonds admittedly
It is fixed etc., preferably it is adhesively fixed.
" end interconnection " alleged in context, refers to sample pad, coated film, the end of the several parts of adsorptive pads
Physically interconnect, its connected mode includes but is not limited to overlap, is bonded, is laminated connection etc., as long as can allow for chromatographing sample
Product continuously sequentially pass through various pieces.
" adsorptive pads " alleged in context, refer to the sheet knot of the absorbent material composition commonly used in immunochromatography technique
Structure, causes the reaction solution containing sample to be moved to adsorptive pads from loading pad along chromatography direction because of the capillarity of its micro-structural.
Adsorptive pads may be selected from absorbent filter etc..
In test strips of the invention, membrane body can be the immobilon-p commonly used in immunochromatography technique, such as glass fibre
Plain film (fiberglass), nylon membrane (nylon), polyvinylidene fluoride film (polyvinylidene fluoride, PVDF) film,
Nitrocellulose filter (nitrocellulose, NC) etc., preferably nitrocellulose filter.The film can have 0.2-0.6 μm or
5-10 μm of aperture.
Present invention also offers a kind of kit for detecting CRP concentration, comprising test strips of the invention and independent bag
The example reaction liquid of dress, the example reaction liquid comprising with label CRP monoclonal antibody B, and with label with
The material of the Quality Control coating thing specific binding.
In kit for detecting CRP concentration of the invention, the material specifically bound with Quality Control coating thing is
Rabbit igg, anti-rabbit IgG, Avidin, biotin are combined with albumen of biotin or Avidin etc., preferably rabbit igg.
In kit for detecting CRP concentration of the invention, the label in example reaction liquid is fluorescence, chemiluminescence
And/or chromogenic label thing, more preferably preferably fluorescent microsphere, fluorescein, collaurum, rhodamine, fluorescent microsphere, most preferably wherein wrap
Bury the fluorescent microsphere of rare-earth europium Eu.
In kit for detecting CRP concentration of the invention, the reaction solution includes 0.002-0.02OD600Fluorescence mark
The rabbit igg and 0.01-0.05OD of note600The CRP monoclonal antibody B of fluorescence labeling, preferably 0.003-0.01OD600Fluorescence labeling
Rabbit igg and 0.015-0.03OD600The CRP monoclonal antibody B of fluorescence labeling.It will be appreciated by those skilled in the art that described herein
" CRP monoclonal antibody A " and " CRP monoclonal antibody B " are not intended to carry out any kind of tool to the CRP monoclonal antibody
Body is limited, it is only for made a distinction between two kinds of CRP monoclonal antibodies.That is, in the example reaction liquid
Both CRP monoclonal antibody A for being used for coated film in CRP monoclonal antibody B and test strips may each be any CRP monoclonals and resist
Body, as long as they are respectively in connection with the different loci of CRP.
In an implementation method for detecting CRP concentration of the invention, diluted with example reaction liquid contain CRP first
Sample, now CRP combined with the CRP monoclonal antibody B of the fluorescence labeling in reaction solution, formed fluorescence labeling-monoclonal antibody B-CRP
Compound.After being loaded onto in sample pad, reactant mixture enters coated film along chromatography direction.Reactant mixture is chromatographed by C lines
When, the rabbit igg anti-rabbit IgG coated with C lines of fluorescence labeling forms fluorescence labeling-rabbit igg-anti-rabbit IgG compounds and stays in C lines
Place；Reactant mixture is chromatographed during by T1 lines, the CRP of fluorescence labeling-monoclonal antibody B-CRP compounds and T1 lines in reactant mixture
Monoclonal antibody A is combined, and is formed the double-antibody sandwich compound with fluorescence labeling and is stayed at T1 lines；Reactant mixture chromatography warp
When crossing T2 lines, the CRP antigens dissociated in reactant mixture be coated on it is glimmering in the CRP antigenic competition association reaction mixtures of T2 lines
The CRP monoclonal antibody B of signal forming fluorescence labeling-monoclonal antibody B-CRP compounds, be coated on the CRP antigens of T2 lines with it is glimmering
Fluorescence labeling-monoclonal antibody B-CRP compounds that the CRP monoclonal antibody B of signal is formed are left at T2 lines, remaining fluorescence mark
Note-monoclonal antibody B-CRP compounds are not left at T2 lines then.The fluorescence intensity signals value of coated film is detected by fluorescence detector,
Normative reference curve, you can obtain the CRP detectable concentrations in sample.
By the C lines described in leading portion, T1 lines, the special arrangement mode of T2 lines and its with example reaction liquid and sample in
The interaction mode of CRP, test strips of the invention overcome existing immuno-chromatographic test paper strip in differential responses temperature with kit
Degree is lower or carries out the defect that reading after the differential responses time is not sufficiently stable, and realization still ensures that inspection under various unfavorable testing conditions
Survey the technique effect of stability and precision.Test strips of the invention and kit thus there is more preferably extensive suitable environment,
For the CRP detections of basic hospital, chaos caused by war, unstable region, extreme environment provide preferably selection.
Brief description of the drawings
Fig. 1：A schematic diagram for the immuno-chromatographic test paper strip for detecting CRP concentration of specific embodiment of the invention.
1- base plates, 2- sample pads, 3- coated films, 4- adsorptive pads, 5- Quality Controls coating thing coating area (C lines), 6-CRP monoclonal antibody A bags
By area (T1 lines), 7-CRP antigen coats area (T2 lines).
Fig. 2：The signal value for calculating a CRP concentration-CRP concentration standard curve for specific embodiment of the invention.
Implementation method
Include following embodiment herein, for becoming apparent from, being explicitly described technical side of the invention in an exemplary manner
Case.Those skilled in the art should be appreciated that according to disclosure herein can be permitted in disclosed specific implementation method
It is change but still obtain similar or similar result more, without departing from thought of the invention and scope.Specific reality of the invention
The mode of applying is only used for explaining the present invention, and is not intended to limit the present invention by any mode.
Material and method
The technological means used in specific embodiment, refer to standard laboratory manual, for example《DNA techniques hand
Volume》, on April 1st, 2002, Science Press.
Unless otherwise instructed, the reagent for being used in specific embodiment is purchased from the Sichuan mikey biology limited public affairs of new material technology
Department.
Embodiment 1
Prepare test strips of the invention
First, the preparation of antibody
CRP antigens used, rabbit igg, anti-rabbit IgG, CRP monoclonal antibody A (article No.s in embodiment：) and CRP is mono- XCL1146
Clonal antibody B (article No.s：XCL1145) purchased from Sichuan mikey biology new material technology Co., Ltd.
2nd, the preparation of coated film
1. coating buffer solution is prepared
Coating buffer solution C is the 10mM PBS solutions containing 2% trehalose and 0.5mg/mL anti-rabbit IgG, is coated with buffer solution
T1 is the 10mM PB solution containing 3% methyl alcohol and 2.0mg/mL CRP monoclonal antibodies A, and coating buffer solution T2 is to contain 2% sea
The 10mM PBS solutions of algae sugar and 1.0mg/mLCRP antigens.
2. film is sprayed
Using Membrane jetter (company：BioDot, model：BioDot xyz3060), sequentially coating buffer solution C (is coated with dense
Degree 0.5mg/mL), coating buffer solution T1 (coating concentration 2.0mg/mL) and coating buffer solution T2 (being coated with concentration 1.0mg/mL) exists
Rule on nitrocellulose filter, C lines, T1 lines and T2 lines are formed respectively.Adjacent coating area (i.e. C lines and T1 lines, T1 lines and T2 lines)
Between at intervals of 3mm, two, outside coating area (C lines and T2 lines) reserves enough spaces with the outermost edges of coated film,
For overlap joint sample pad and adsorptive pads (see Fig. 1).The nitrocellulose filter sprayed after film is put into 40 DEG C of vacuum drying chamber and is done
Taken out after dry 30 minutes, sealed standby.
3rd, the preparation of sample pad
With aqueous solution soaking glass fibre element film (being purchased from PALL) containing 0.1%TW-20.After being thoroughly impregnated with, film is put into
In 55 DEG C of vacuum drying chamber, taken out after drying 40 minutes, sealed standby.
4th, the preparation of test strips
The coated film that will be made is pasted onto the center (see Fig. 1) of PVC base plates.Sample pad is pasted on the outside of C lines, makes sample
Product pad 1-2mm Chong Die with coated film overlapping edges on the outside of C lines；Adsorptive pads are pasted on the outside of T2 lines, makes adsorptive pads with T2 lines outside
Coated film overlapping edges overlap 1-2mm.
Embodiment 2
Prepare example reaction liquid
First, rabbit igg and CRP monoclonal antibody B are marked with fluorescent microsphere
Using 0.5 μm of rare-earth fluorescent microballoon, it is coupled by covalent bond and rabbit igg and CRP monoclonal antibody B, fluorescence is obtained
The rabbit igg and CRP monoclonal antibody B of microballoon mark.
2nd, example reaction liquid is prepared
With containing 5%NaCl, 0.5% bovine serum albumin(BSA), 0.5% casein, 5% trehalose, 0.5%PEG20000,
Rabbit igg that 0.1M phosphate buffers (pH 7.6) dilution of 0.2%Tween20,0.5%PC300 fluorescent microsphere is marked with
The CRP monoclonal antibody B marked with fluorescent microsphere so that the rabbit igg of fluorescence labeling is final concentration of in final product
0.005OD600, the final concentration of 0.02OD of the CRP monoclonal antibody B of fluorescence labeling600.It is mounted in the volume integral of 0.745ml/ pipes
In EP pipes and seal.It is stored refrigerated at 4 DEG C.
Comparative example 1
TC formula test strips
Purchase Guangzhou Wondfo Biotech. Co., Ltd. whole process C reactive protein detection reagent (lot number：C15907
B), detected as TC formula test strips.The T lines of the test strips are coated with C reactive protein monoclonal antibody, and C lines are coated with anti-
Mouse IgG polyclonal antibodies.
Comparative example 2
Prepare TTT formula test strips
The test strips of comparative example 2 are prepared as described in example 1 above, and difference is sequentially to be arranged along chromatography direction on coated film
T1 lines apart from one another by 5mm, T2 lines and T3 lines are arranged, wherein T1 lines are coated with CRP monoclonal antibody A (3mg/mL), T2 line bags
There are CRP monoclonal antibody B (5mg/mL), T3 lines to be coated with CRP antigens (5mg/mL).The standard detection temperature of the test strips is
18 DEG C~30 DEG C, the standard detection time is 3 minutes.
Embodiment 3
CRP concentration is detected at different ambient temperatures
First, the standard curve of test paper of the present invention is drawn
Using 20M PB buffer solutions dilute concentration known commercially available CRP products (Sichuan mikey biology new material technology it is limited
Company), obtain the concentration gradient standard items as shown in table 1 from 0.00mg/L to 320mg/L.Test strips of the present invention after by loading
It is placed in fluorescent quantitation spectral detection system (R01 dry type fluorescence immunity analyzers, Sichuan Maker Biological Science and Technology Co., Ltd.)
In, obtain fluorescence signal intensity signal value reading.With signal value as X-axis, concentration be Y-axis draw standard curve, obtain Fig. 2.
Table 1, test strips standard curve of the present invention
| Concentration mg/L | Signal value |
| 0.00 | 36 |
| 0.47 | 139 |
| 0.94 | 251 |
| 1.88 | 440 |
| 3.75 | 823 |
| 7.50 | 1637 |
| 15 | 3234 |
| 30 | 6662 |
| 60 | 12138 |
| 120 | 18889 |
| 240 | 29442 |
| 320 | 35149 |
2nd, the preparation of reference sample solution
Using the commercially available CRP products (Sichuan mikey biology new material technology Co., Ltd) of concentration known, table 2 below, 3 are prepared
The reference sample solution of CRP concentration (mg/L) listed by middle left column, for follow-up measure.
3rd, Sample Dilution
Using obtained example reaction liquid in embodiment 2, with 1：150 dilution proportion reference sample solution.
4th, detect
The reference sample solution that will be diluted by reaction solution is loaded in embodiment 1 (the μ L of loading volume 90), comparative example 1 respectively
On the loading pad of the test strips of (the μ L of loading volume 75) and comparative example 2 (the μ L of loading volume 90), chromatography is set to react respectively at three
Carried out at different temperature 3 minutes；By test strips be placed in fluorescent quantitation spectral detection system (R01 dry type fluorescence immunity analyzers,
Sichuan Maker Biological Science and Technology Co., Ltd.) in, read fluorescence signal value reading；The standard of fluorescence signal value and Fig. 2 is bent
Line is compared and obtains CRP measure concentration.The results are shown in Table 2.
Table 2
Even if can be seen that test strips of the invention under 6.2 DEG C and 37 DEG C of unfavorable temperature conditionss by the data of table 2
When being reacted, reliable and stable testing result is maintained to.But, comparative example 1 and comparative example 2 are deviateing environment temperature
Unfavorable temperature (6.2 DEG C and 37 DEG C) under, the CRP concentration that obtains of detection much deviates standard concentration, no longer with inspection
The reference value surveyed in meaning.
Embodiment 4
CRP concentration is detected after the differential responses time
Reference sample solution is prepared as described in example 3 above and it is diluted, the difference is that only detection part
It is carried out as follows.
The reference sample solution that will be diluted by reaction solution is loaded in embodiment 1 (the μ L of loading volume 90), comparative example 1 respectively
On the loading pad of (the μ L of loading volume 75) and comparative example 2 (the μ L of loading volume 90), make chromatography react carry out 2 respectively at 25 DEG C,
3rd, 4,5 or 6 minutes；Test strips are placed in fluorescent quantitation spectral detection system (R01 dry type fluorescence immunity analyzers, Sichuan mikey
Biotech inc) in, read fluorescence signal value；The standard curve of fluorescence signal value and Fig. 2 is compared
Concentration is determined to CRP.The results are shown in Table 3.
Table 3.
Even if can be seen that test strips of the invention than standard detection time 3min earlier by the data of table 3
When the time point of (2min) or more late (4,5,6min) carries out reading, the adverse effect to testing result is also far smaller than existing
CRP test strips.And for comparative example 1 and comparative example 2, in the time than standard detection time 3min much earlier or later
When point carries out reading, the CRP concentration for obtaining much has deviateed the concentration of standard items, no longer with the reference price on Clinical significance of detecting
Value.
As can be seen here, test strips of the present invention can be even broader anti-under 0 DEG C to 40 DEG C of temperature range, at 2-6 minutes
In the range of between seasonable, accurately whole process CRP Concentration Testings are realized.Test strips of the invention are in the temperature beyond normal operating range
Under time conditions, the detection performance with relatively reliable stabilization.
Claims (11)
1. a kind of immuno-chromatographic test paper strip for detecting CRP concentration, is included on base plate sequentially fixed and end and interconnects
Sample pad, coated film, adsorptive pads, sequentially divide on membrane body along the direction from sample pad to adsorptive pads on the coated film
It is furnished with Quality Control coating thing coating area, CRP monoclonal antibody A coatings area and the CRP antigen coats area being spaced from.
2. test strips according to claim 1, wherein Quality Control coating thing is selected from anti-rabbit IgG, rabbit igg, biotin, parent
With element or be combined with the albumen of biotin or Avidin, preferably anti-rabbit IgG.
3. test strips according to claim 1 and 2, wherein the coating concentration of Quality Control coating thing is 0.1-10mg/mL,
It is preferred that 0.6-4mg/mL.
4. test strips according to any one of Claim 1-3, wherein the coating concentration of the CRP monoclonal antibody A is
0.5-10mg/mL, preferably 1-4mg/mL.
5. test strips according to any one of claim 1 to 4, wherein the coating concentration of the CRP antigens is 0.1-
10mg/mL, preferably 0.6-2mg/mL.
6. test strips according to any one of claim 1 to 5, wherein Quality Control coating thing coating area and CRP Dan Ke
Spacing distance between grand antibody A coating area is 2-8mm, preferably 2-4mm.
7. test strips according to any one of claim 1 to 6, wherein the CRP monoclonal antibody A coatings area and CRP
Spacing distance between antigen coat area is 2-8mm, preferably 2-4mm.
8. a kind of kit for detecting CRP concentration, comprising the test strips as any one of claim 1 to 7 and solely
The example reaction liquid of vertical packaging, the example reaction liquid includes the CRP monoclonal antibody B with label, and with label
With the Quality Control coating thing specifically bind material.
9. it is used to detect the kit of CRP concentration as claimed in claim 8, wherein described specific with Quality Control coating thing
With reference to material be rabbit igg, anti-rabbit IgG, Avidin, biotin or be combined with albumen of biotin or Avidin etc., preferably rabbit
IgG。
10. it is used to detect the kit of CRP concentration as claimed in claim 8 or 9, wherein the label is fluorescence, chemistry
Luminous and/or chromogenic label thing, preferably fluorescent microsphere, fluorescein, collaurum, rhodamine, more preferably fluorescent microsphere, most preferably its
In be embedded with the fluorescent microsphere of rare-earth europium Eu.
11. kit for detecting CRP concentration as any one of claim 8-10, wherein the example reaction liquid
Comprising 0.002-0.02OD600The rabbit igg and 0.01-0.05OD of fluorescence labeling600The CRP monoclonal antibody B of fluorescence labeling, preferably
Ground includes 0.003-0.01OD600The rabbit igg and 0.015-0.03OD of fluorescence labeling600The CRP monoclonal antibody B of fluorescence labeling.
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| CN108469518A (en) * | 2018-03-20 | 2018-08-31 | 迈克生物股份有限公司 | A kind of cystatin C fluorescence immune chromatography detection test strips and its application |
| CN109725154A (en) * | 2018-10-08 | 2019-05-07 | 杭州康知生物科技有限公司 | A kind of IgG4 fluorescence immune chromatography assay kit and measuring method |
| CN110261622A (en) * | 2019-07-23 | 2019-09-20 | 珠海科域生物工程股份有限公司 | A kind of fecal sample transferrins Test paper and preparation method thereof |
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| CN113588960A (en) * | 2020-11-04 | 2021-11-02 | 北京北方生物技术研究所有限公司 | Immunochromatography detection test strip by ratio fluorescence method and detection method thereof |
| CN113504377A (en) * | 2021-07-15 | 2021-10-15 | 中国科学院长春应用化学研究所 | Test strip for bimodal detection of CRP (C-reactive protein), preparation method and detection method |
| CN113391076A (en) * | 2021-07-20 | 2021-09-14 | 深圳健丰源科技有限公司 | Immunodetection method of 25-hydroxy vitamin D and application thereof |
| CN113759129A (en) * | 2021-09-26 | 2021-12-07 | 北京倍肯恒业科技发展股份有限公司 | Rapid detection card for leucocyte-bound C-reactive protein and preparation method thereof |
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