CN209024539U - Denitrification culture apparatus in multichannel room - Google Patents
Denitrification culture apparatus in multichannel room Download PDFInfo
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- CN209024539U CN209024539U CN201821675933.0U CN201821675933U CN209024539U CN 209024539 U CN209024539 U CN 209024539U CN 201821675933 U CN201821675933 U CN 201821675933U CN 209024539 U CN209024539 U CN 209024539U
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Abstract
Denitrification culture apparatus in a kind of multichannel room disclosed by the utility model, it includes culture tube assembly, pipe support, thermostat and magnetic stirring apparatus, tube assembly is cultivated to be arranged on pipe support, the inside of thermostat is arranged in pipe support, thermostatted water is provided in thermostat, magnetic stirring apparatus is arranged between adjacent culture tube assembly；The circumferential direction of the top cover and bottom cover of cultivating tube assembly is provided with card slot, and sealing ring is arranged between card slot and inboard wall of tube body；The strut of pipe support is arranged between upper disk and lower wall；Tube assembly is cultivated to pass through the limit hole on upper disk and be clamped by the supported hole on lower wall.Denitrification culture apparatus is for deposit denitrification, anaerobic ammonium oxidation and fixed nitrogen rate determination in a kind of multichannel room provided by the utility model, its is structurally reasonable, collection culture tube, magnetic agitation, sampling, temperature are controlled in one, denitrification culture in the state of totally-enclosed, avoid extraneous interference, to carry out Multi-example continuous culture simultaneously and continuous sampling measurement.
Description
Technical field
The utility model relates to denitrification culture apparatus in nitrogen cycle technical field more particularly to a kind of multichannel room.
Background technique
Nitrogen isotope tracer technique is the effective means of ocean nitrogen cycle research, has been widely used in marine organisms at present
Geochemical research.And the measurement of nitrogen isotope is the key point of tracer technique.Nitrogen cycle is by each of microorganism catalysis
Conversion process between kind nitrogenous compound.Nitrogen compound is one of important nutritive salt of marine organism growth, they regulate and control
The primary productivity in the most of sea areas in the world is horizontal.Ocean primary production process can absorb Atmospheric CO by the effect of bio-pump2,
The weather of the earth is influenced, while the organic matter synthesized by it is the substance source of petroleum deposit deposition, and some microbial metabolisms
Compound gas caused by process is also greenhouse gases, is equally also had an impact to climate change.Therefore, understand marine environment
In nitrogen cycle be of great significance.
Ocean Nitrogen budget whether balance to be in the studying for a long period of time of ocean nitrogen cycle it is up to the present one still unsolved
Problem.In traditional view, biological nitrogen fixation is considered as the main source of bioavailability nitrogen, and denitrification and
Newfound anaerobic ammonium oxidation process is considered as the main source of utilizability nitrogen in marine environment.
Therefore, the N for measurement is obtained2, the reasonable, reliable of culture apparatus, stability, which seem, to be even more important.It needs to set
Denitrification culture apparatus in a kind of multichannel room is counted, for deposit denitrification, anaerobic ammonium oxidation and fixed nitrogen rate determination.
Utility model content
The purpose of this utility model is anti-nitre in a kind of multichannel room provided by the utility model in view of the above technical problems
Change culture apparatus, it is structurally reasonable for deposit denitrification, anaerobic ammonium oxidation and fixed nitrogen rate determination, collect culture tube, magnetic force
Stirring, sampling, temperature are controlled in one, denitrification culture in the state of totally-enclosed, avoid extraneous interference, more to carry out
Sample continuous culture simultaneously and continuous sampling measurement.
In order to solve the above technical problems, denitrification culture apparatus in a kind of multichannel room provided by the utility model, packet
Culture tube assembly, pipe support, thermostat and magnetic stirring apparatus are included, the culture tube assembly is arranged on pipe support, and pipe support setting exists
The inside of thermostat, thermostatted water is provided in thermostat, and magnetic stirring apparatus is arranged between adjacent culture tube assembly；The training
Feeding tube assembly includes top cover, tube body and bottom cover, and the circumferential direction of top cover and bottom cover is provided with card slot, and sealing ring is arranged in card slot and tube body
Between inner wall；The pipe support includes upper disk, strut and lower wall, and strut is arranged between upper disk and lower wall；The culture tube assembly
Limit hole on upper disk is simultaneously clamped by the supported hole on lower wall；Magnetic stirring apparatus includes magnet and bar magnet, magnet setting
It is enclosed in loader and is placed in the tube body in culture tube assembly on upper disk in the drive shaft of motor, on the outside of bar magnet.
In some embodiments, the top cover is cylindrical body, is provided centrally with sampling hole, bottom is provided with bending
Face；It is provided with press mounting hole at the top of the top cover, is symmetricly set on the two sides of sampling hole.
In some embodiments, the circumferential direction of cylindrical body is arranged in the card slot, and quantity is multiple.
In some embodiments, the sampling hole is through-hole, is communicated with the bending face of cap base；The press mounting hole is
Blind hole.
In some embodiments, the bottom cover includes cylinder, limit plate and positioning column, is integrated, limit plate
Top be provided with clamping groove, the end of tube body is arranged in clamping groove.
In some embodiments, the circumferential direction of cylinder is arranged in the card slot, and quantity is multiple.
In some embodiments, the tube body is quartz ampoule, and top cover and bottom cover are polytetrafluoroethylene (PTFE).
In some embodiments, the limit hole and supported hole are multiple groups, to fixed multiple groups culture tube assembly.
The utility model the utility model has the advantages that
Denitrification culture apparatus is used for deposit denitrification, anaerobic ammonium oxygen in a kind of multichannel room provided by the utility model
Change and fixed nitrogen rate determination, have following technical effect that
(1) the application collection culture tube, magnetic agitation, sampling, temperature are controlled in one, denitrification in the state of totally-enclosed
Culture avoids extraneous interference, it is ensured that good test effect；
(2) the application can carry out multiple groups sample and cultivate under same environment, and the top cover of innovation and its sealing take with piston
Sample design, ensure that the convenience of continuous sampling at any time, reduces error, provide condition for the research of denitrification process.
(3) unique agitating device can make water body in elongated culture tube uniform, and will not disturb to deposit,
Concentration of substrate will not be changed, keep the uniformity of aqueous phase system, improve the accuracy of sample detecting result.
Detailed description of the invention
It will be apparent and more hold by made detailed description, the above-mentioned advantage of the utility model in conjunction with the following drawings
Readily understood, these attached drawings are only schematical, are not intended to limit the utility model, in which:
Fig. 1 is the structural schematic diagram of denitrification culture apparatus in a kind of multichannel room described in the utility model；
Fig. 2 is the structural schematic diagram of denitrification culture apparatus in the corresponding multichannel room without thermostat Fig. 1；
Fig. 3 is the structural schematic diagram of the pipe support of the utility model；
Fig. 4 is the structural schematic diagram of the culture tube assembly of the utility model；
Fig. 5 is the corresponding explosive view of Fig. 4；
Fig. 6 is the structural schematic diagram of the top cover of the utility model；
Fig. 7 is the structural schematic diagram of the bottom cover of the utility model.
In figure:
10. cultivating tube assembly；11. top cover.111. sampling hole, 112. press mounting holes；12. tube body；13. bottom cover, 131. main bodys,
132. limit plate, 133. positioning columns, 134. clamping grooves；14. card slot；
20. pipe support；Disk on 21., 211. limit holes；22. pillar；23. lower wall, 231. supported holes；
30. thermostat；31. circulation line；32. temperature controller；
40. magnetic stirring apparatus；41. magnet；42. bar magnet；43. motor；44. loader；
50. sealing ring.
Specific embodiment
Fig. 1 to Fig. 7 is a kind of accompanying drawings of denitrification Culture in situ device described in the utility model, below with reference to tool
Body embodiment and attached drawing, are described in detail the application.
The embodiment recorded herein is the specific specific embodiment of the utility model, for illustrating the utility model
Design, be it is explanatory and illustrative, should not be construed as the limit to the utility model embodiment and the scope of the utility model
System.In addition to the embodiment recorded herein, those skilled in the art can also be public based on the claim of this application book and specification institute
For the content opened using obvious other technical solutions, these technical solutions include using doing to the embodiment recorded herein
The technical solution of any obvious substitutions and modifications out.
The attached drawing of this specification is schematic diagram, aids in illustrating the design of the utility model, it is schematically indicated each section
Shape and its correlation.It note that for the ease of clearly showing the structure of each component of the utility model embodiment, phase
Same reference marker is for indicating identical part.
The structural schematic diagram of denitrification culture apparatus in a kind of herein described multichannel room, as shown in Figure 1, its specific packet
Culture tube assembly 10, pipe support 20, thermostat 30 and magnetic stirring apparatus 40 are included, the culture tube assembly 10 is arranged in pipe support 20
On, the inside of thermostat 30 is arranged in pipe support 20, and thermostatted water is provided in thermostat 30, and magnetic stirring apparatus 40 is arranged adjacent
It cultivates between tube assembly 10；In the embodiment shown in fig. 1, thermostat 30 is internally provided with thermostatted water, in circulation line 31
There is recirculated water, temperature controller 32 is provided on circulation line 31, to accurately control thermostat temperature, ensures denitrification culture
Locating experimental condition.
In the application, the culture tube assembly 10 includes top cover 11, tube body 12 and bottom cover 13, as shown in Fig. 4, top cover 11
And the circumferential direction of bottom cover 13 is provided with card slot 14, sealing ring 50 is arranged between 12 inner wall of card slot 14 and tube body；
In some embodiments, the pipe support 20 includes upper disk 21, strut 22 and lower wall 23, and strut 22 is arranged in upper disk
Between 21 and lower wall 23, as shown in Figure 2；The culture tube assembly 10 passes through the limit hole 211 on upper disk 21 and by lower wall 23
Supported hole 231 be clamped, as shown in Figure 3；
Traditional agitating mode is that mechanical stirring or lateral fixed point magnetic stirring, both modes are difficult the water in elongated tubular
It mixes, and since for deposit in order to measure the nitrogen cycle in water body disturbance should be avoided in the tube body lower part of this culture tube assembly
Deposit, traditional magnetic agitation are that bar magnet is made to move in a circle in container bottom, can not be done to elongated tubular upper space effectively
Stirring, and disturb acutely, the utility model is innovated in the following way, and magnetic stirring apparatus 40 includes magnet 41 and bar magnet
42, magnet 41 is arranged on upper disk 21 in the drive shaft of motor 43, and the outside of bar magnet 42 is enclosed in loader 44 and is placed on
It cultivates in the tube body 12 in tube assembly 10, as shown in Figs. 1-5.Bar magnet can choose it is half-moon-shaped so that bar magnet can be with tube wall veneer
It coincide, improves mixing effect, loader 44 can choose the shape for being adapted to bar magnet, and material is polytetrafluoroethylene (PTFE).It is shown in Fig. 1
Embodiment in, under the drive of 43 drive shaft of motor, the magnet 41 in drive shaft is being moved up and down, drive tube body 12 in
Bar magnet 42 moves up and down, to achieve the purpose that stir the culture solution in tube body 12.In this way, water body can be made uniform, and will not be right
Deposit is disturbed, and concentration of substrate will not be changed, and keeps the uniformity of aqueous phase system, improves the accurate of sample detecting result
Property.
And it is possible to the distance between culture tube assembly suitably be adjusted, so that the stirring of adjacent culture tube assembly only needs
It wants one group of motor 43 to drive by magnet 41, to improve efficiency, device complexity is reduced, convenient for the maintenance in later period.
In the application, the top cover 11 is cylindrical body, is provided centrally with sampling hole 111, and bottom is provided with bending face,
To guarantee to make after filling sample water full of tube body 12 without spacing；The top of the top cover 11 is provided with press mounting hole 112, symmetrical
The two sides of sampling hole 111 are set, as shown in Figure 6；The sampling hole 111 is through-hole, the bending face phase with 11 bottom of top cover
It is logical；The press mounting hole 112 is blind hole.The card slot 14 is arranged in the circumferential direction of cylindrical body, quantity be it is multiple, to guarantee top cover 11
With the leakproofness of tube body 12, in the embodiment shown in fig. 5, the quantity of card slot 14 is three, and sealing ring 50 is silica gel, is set
It sets between card slot 14 and the inner wall of tube body 12.
Fig. 7 is the structural schematic diagram of the bottom cover of the utility model, and the bottom cover 13 includes cylinder 131, limit plate 132 and determines
Position column 133, is integrated；The top of the limit plate 132 is provided with clamping groove 134, and the end setting of tube body 12 exists
In clamping groove 134, the setting of tube body 12 is in card section behaviour 134；The circumferential direction of cylinder 131 is arranged in the card slot 14, and quantity is more
A, to guarantee the leakproofness of bottom cover 13 Yu tube body 12, in the embodiment shown in fig. 5, the quantity of card slot 14 is three, sealing
Circle 50 is silica gel, is arranged between card slot 14 and the inner wall of tube body 12.
In some embodiments, the tube body 12 is quartz ampoule, and top cover 11 and bottom cover 13 are polytetrafluoroethylene (PTFE), sealing ring 50
Using silica gel, in this way can to avoid culture apparatus component on denitrifying influence, the smooth development of guarantee test.
The use step of denitrification culture apparatus in multichannel room is briefly stated below:
Firstly, one or more tube bodies 12 are inserted into deposit, insertion depth is about 10cm, and tube body 12 is extracted in rotation, fastly
Bottom cover 13 with sealing ring 50 is mounted on the lower part of tube body 12 by speed；
Then, marker is added, such as 99% high abundance15N potassium nitrate, then the top cover 11 with sealing ring 50 is mounted on
The top of tube body 12, light pressure go out air, tighten sample cock, are put on pipe support 20；
Then, start the temperature controller 32 on thermostat 30, set cultivation temperature, cultivated, sampling is opened in timing
Valve carries out water sampling；Sampling uses gland mode in the application, and light pressure sampling compression bar flows out water sample automatically into sampling
Bottle；It will specifically sample on the press mounting hole 112 on compression bar insertion top cover 11, open sample cock, top cover 11 is equivalent to one
Piston, water sample is upper with sampling hole 111, flow to the hose connecting with sample cock by sample cock, eventually enters into sampling
Bottle.
The sample of acquisition can send to laboratory and carry out15N isotope assay, and carry out denitrification rate estimation and related point
Analysis.
Compared with prior art, denitrification culture apparatus is used for deposit in a kind of multichannel room provided by the utility model
Denitrification, anaerobic ammonium oxidation and fixed nitrogen rate determination, structurally reasonable, collection culture tube, magnetic agitation, sampling, temperature are controlled in
One, denitrification culture in the state of totally-enclosed avoid extraneous interference, to carry out Multi-example continuous culture simultaneously, and
Continuous sampling measurement.
The utility model is not limited to above embodiment, anyone can obtain other under the enlightenment of the utility model
Various forms of products, however, make any variation in its shape or structure, it is all with identical or similar to the present application
Technical solution, all fall within the protection scope of the utility model.
Claims (8)
1. denitrification culture apparatus in a kind of multichannel room, which is characterized in that including culture tube assembly (10), pipe support (20), perseverance
Warm slot (30) and magnetic stirring apparatus (40), the culture tube assembly (10) are arranged on pipe support (20), and pipe support (20) setting exists
The inside of thermostat (30), thermostat (30) is interior to be provided with thermostatted water, and magnetic stirring apparatus (40) is arranged in adjacent culture tube group
Between part (10)；The culture tube assembly (10) includes top cover (11), tube body (12) and bottom cover (13), top cover (11) and bottom cover
(13) circumferential direction is provided with card slot (14), and sealing ring (50) is arranged between card slot (14) and tube body (12) inner wall；The pipe support
It (20) include upper disk (21), strut (22) and lower wall (23), strut (22) is arranged between upper disk (21) and lower wall (23)；It is described
Tube assembly (10) are cultivated to pass through the limit hole (211) on upper disk (21) and be clamped by the supported hole (231) on lower wall (23)；Magnetic force
Agitating device (40) includes magnet (41) and bar magnet (42), and the drive shaft of motor (43) is arranged on upper disk (21) in magnet (41)
On, it is enclosed in loader (44) and is placed in the tube body (12) in culture tube assembly (10) on the outside of bar magnet (42).
2. denitrification culture apparatus in multichannel room according to claim 1, which is characterized in that the top cover (11) is circle
Cylinder, is provided centrally with sampling hole (111), and bottom is provided with bending face；Press fitting is provided at the top of the top cover (11)
Hole (112), is symmetricly set on the two sides of sampling hole (111).
3. denitrification culture apparatus in multichannel room according to claim 2, which is characterized in that card slot (14) setting
In the circumferential direction of cylindrical body, quantity is multiple.
4. denitrification culture apparatus in multichannel room according to claim 2, which is characterized in that the sampling hole (111)
For through-hole, communicated with the bending face of top cover (11) bottom；The press mounting hole (112) is blind hole.
5. denitrification culture apparatus in multichannel room according to claim 1, which is characterized in that the bottom cover (13) includes
Cylinder (131), limit plate (132) and positioning column (133), are integrated, and the top of limit plate (132) is provided with clamping
Slot (134), the end setting of tube body (12) is in clamping groove (134).
6. denitrification culture apparatus in multichannel room according to claim 5, which is characterized in that card slot (14) setting
Circumferential direction in cylinder (131), quantity are multiple.
7. denitrification culture apparatus in multichannel room according to claim 1, which is characterized in that the tube body (12) is stone
Ying Guan, top cover (11) and bottom cover (13) are polytetrafluoroethylene (PTFE).
8. denitrification culture apparatus in multichannel room according to claim 1, which is characterized in that the limit hole (211)
It is multiple groups with supported hole (231), to fixed multiple groups culture tube assembly (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821675933.0U CN209024539U (en) | 2018-10-16 | 2018-10-16 | Denitrification culture apparatus in multichannel room |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821675933.0U CN209024539U (en) | 2018-10-16 | 2018-10-16 | Denitrification culture apparatus in multichannel room |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209024539U true CN209024539U (en) | 2019-06-25 |
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ID=66906401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821675933.0U Active CN209024539U (en) | 2018-10-16 | 2018-10-16 | Denitrification culture apparatus in multichannel room |
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| Country | Link |
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| CN (1) | CN209024539U (en) |
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2018
- 2018-10-16 CN CN201821675933.0U patent/CN209024539U/en active Active
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