CN104459081A - Determination method for activated sludge respiration rate and in-situ respiration rate instrument thereof - Google Patents
Determination method for activated sludge respiration rate and in-situ respiration rate instrument thereof Download PDFInfo
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- CN104459081A CN104459081A CN201310424943.2A CN201310424943A CN104459081A CN 104459081 A CN104459081 A CN 104459081A CN 201310424943 A CN201310424943 A CN 201310424943A CN 104459081 A CN104459081 A CN 104459081A
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- respiratory rate
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- dissolved oxygen
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- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000010802 sludge Substances 0.000 title claims abstract description 20
- 230000029058 respiratory gaseous exchange Effects 0.000 title abstract 6
- 238000011065 in-situ storage Methods 0.000 title abstract 4
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 33
- 239000001301 oxygen Substances 0.000 claims abstract description 33
- 238000003756 stirring Methods 0.000 claims abstract description 19
- 239000000523 sample Substances 0.000 claims abstract description 16
- 230000036387 respiratory rate Effects 0.000 claims description 71
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 31
- 230000008859 change Effects 0.000 claims description 12
- 238000005259 measurement Methods 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 18
- 238000005070 sampling Methods 0.000 abstract description 8
- 230000004044 response Effects 0.000 abstract description 4
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000003556 assay Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 3
- 230000036284 oxygen consumption Effects 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000009533 lab test Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011112 process operation Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000012956 testing procedure Methods 0.000 description 2
- 241001494479 Pecora Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Landscapes
- Activated Sludge Processes (AREA)
Abstract
The invention discloses a determination method for activated sludge respiration rate and an in-situ respiration rate instrument thereof. The respiration rate instrument comprises a reactor, a support rod, a stirring device, a dissolved-oxygen probe and a data acquisition device; the reactor comprises a foldable pedestal connected with the support rod; the stirring device is arranged in the reactor; and the dissolved-oxygen probe is arranged in the reactor and is connected with the data acquisition device. The method and the instrument can realize in-situ measuring at a test point in a biochemical pool, avoid measure result deviation caused by off-line measure after sampling. The method and the instrument realize in-situ rapid measuring on the respiration rate, are fast in response speed, and only need 1-3 min for each time of measuring. The method and the instrument realize hand-held portable measuring, operation is simple, price is low, and the method and the instrument are convenient for popularization and application.
Description
Technical field
The present invention relates to a kind of assay method and original position respiratory rate instrument thereof of active sludge respiratory rate.
Background technology
Wastewater treatment in China factory, in the urgent need to improving operation level, realizes process stabilizing and runs.Activity at present mainly through observing the result of the microbial reactions such as effluent quality carry out indirect analysis microorganism, lacks the index directly describing microbial activity.Therefore, the on-line monitoring method of exploitation microbial activity and together with, easily-testing and judge the activated state of microorganism, can make up the deficiency of existing process operation index, and to adjusting operational factor, reliable and stable operation, raising effluent quality are significant.The respiratory rate of active sludge refers to the amount of units activity mud at the consumption dissolved oxygen DO of unit interval, and unit is mg/ (L.min) or kg/ (m
3.hr), can microbial activity in concentrated expression Aerobic biological process process and organic matter degradation rate, therefore have a good application prospect.
The measuring method of respiratory rate is many, and laboratory and in-line meter all have maturation method.The method of laboratory comparative maturity is Wadi sheep, needs to use special instrument and equipment.In addition also have closed liquid phase measuring method, measure with dissolved oxygen instrument the rate of change closing dissolved oxygen DO in mixed liquor and calculate respiratory rate, generally only need dissolved oxygen instrument, conical flask and magnetic stirrer.In addition also have the method measuring gas phase carbon dioxide concentration or oxygen consumption volume, carry out indirect calculation dissolved oxygen DO zmount of oxygen consumption.Said method has the Laboratory Instruments of multiple domestic or import, and major defect needs to sample to mud and preserve, cannot portable use at the scene, is difficult to measure the instantaneous respiratory rate under process operation state.
On-line instrument aspect, can be divided into open and closed two kinds of methods.When closed method is measured, inside reactor active sludge and oxygen are isolated, and the changing down of dissolved oxygen concentration equals the respiratory rate of mud.Open measuring method is then carry out aeration continuously, calculates the reactivity parameter of mud by measuring oxygen sag curve.Current existing main on-line instrument has the respirometers such as Merit20, RODTOX, RA-1000, mostly is offshore company's development, expensive, is difficult to apply.In addition, these methods also all need to sample and pre-service to mud, are difficult to test under actual process running status.
In prior art, Chinese patent application 201010579870.0 and 201020650138.3 discloses the converter plant using dissolved oxygen DO and sludge concentration signal to control fan blower, but not respiratory rate testing tool.Chinese patent application 201220526855.4,200810105908.3 and 200810167716.5 is and adopts laboratory method to measure micro-algae, oil pool microorganisms and metazoal respiratory rate.Chinese patent application 200810104254.2 and 200820080009.8 is the method adopting extra electric field to improve microbial activity, tests irrelevant with respiratory rate.Chinese patent application 200910082955.5 and 200910082957.4 is a kind of control method based on respiratory rate, respiratory rate carries out data analysis acquisition by the intermittent aerating in sbr reactor device, do not relate to special respiratory rate proving installation, can not arbitrarily change test position and condition.Chinese patent application 201110195841.9 is the assay method of river oxygen consumption rate constant, does not relate to the method for testing of active sludge, does not also relate to special proving installation.Chinese patent application 200920107719.X is a kind of instructional device utilizing laboratory test philosophy design, can only be used for laboratory demonstration, can not be used for actual field.Chinese patent application 200620004632.6 is a kind of respiratory rate device based on bubbler techniques, can be used for anaerobic and aerobic procedural test, belongs to laboratory test device, be not suitable for the on-the-spot test of sewage treatment plant.
Summary of the invention
The object of this invention is to provide a kind of assay method and original position respiratory rate instrument thereof of active sludge respiratory rate, the present invention can be implemented in the portable measurement in biochemistry pool, obtains microbial activity and influent load running parameter fast.Original position respiratory rate instrument of the present invention according to ripe test philosophy, have structure simple, cheap, test reliable feature, can be used for the multiple requirements such as respiratory rate space distribution, influent load change, oxygenation coefficient mensuration.
A kind of original position respiratory rate instrument provided by the present invention, it comprises a reactor, pole, stirring apparatus, dissolved oxygen probe and data collector;
Described reactor comprises can the base of folding; The base of described reactor is connected with described pole;
Described stirring stirring apparatus is located in described reactor;
Described dissolved oxygen probe is located in described reactor, and it is connected with described data collector.
In above-mentioned original position respiratory rate instrument, described pole is connected with described base by a wire rope.
In above-mentioned original position respiratory rate instrument, described stirring apparatus is located on described base.
In above-mentioned original position respiratory rate instrument, described dissolved oxygen probe is located at the middle and upper part of described reactor.
In above-mentioned original position respiratory rate instrument, the top of described reactor is provided with through hole, can be used for discharging air residual in the mixed liquor in described reactor.
Present invention also offers the method utilizing above-mentioned original position respiratory rate instrument to measure the respiratory rate of mud, comprise the steps:
Described original position respiratory rate instrument is placed in the measurement point of mud, transfers described pole, make the body portion of described base and described reactor from, then the active sludge intermixture of tested point enters in described reactor;
Promote described pole, the main body of described base and described reactor is closed;
Start described stirring apparatus to stir, and simultaneously by the dissolved oxygen concentration of active sludge in reactor described in described data collector record; The dissolved oxygen concentration rate over time of calculated activity mud, the numerical value of the rate of change when described rate of change is stablized is the respiratory rate measuring mud.
In above-mentioned method, the time of described stirring can be 1 ~ 3 minute, in whole whipping process, continues the dissolved oxygen concentration measuring active sludge.
The present invention has following beneficial effect:
Present invention can be implemented in the in site measurement of biochemistry pool build-in test point, avoiding the deviation causing measurement result because sampling rear off-line measurement.The present invention can realize the Quick Measurement of original position respiratory rate, fast response time, and each measurement only needs 1 ~ 3 minute.The present invention can measure by handhold portable, simple to operate, cheap, easy to utilize.
Accompanying drawing explanation
Fig. 1 is the structural representation of original position respiratory rate instrument of the present invention.
Fig. 2 is the pictorial diagram of original position respiratory rate instrument of the present invention.
Fig. 3 is the change curve of the respiratory rate that method of the present invention (in embodiment 1) obtains with classic method.
Fig. 4 is the change curve of the respiratory rate that the embodiment of the present invention 2 obtains.
Fig. 5 is the change curve of the respiratory rate that the embodiment of the present invention 3 obtains.
In figure, each mark is as follows: 1 data collector, 2 dissolved oxygen probes, 3 reactors, 4 bases, 5 poles, 6 wire rope, 7 electric mixing devices.
Embodiment
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment 1, original position respiratory rate instrument
As shown in Figure 1, original position respiratory rate instrument provided by the invention comprises reactor 3, pole 5, electric mixing device 7, dissolved oxygen probe 2 and data collector 1; This reactor 3 comprises one can the base 4 of folding, and pole 5 is connected with base 4 by a wire rope 6, for control base 4 and reactor 3 body between folding; The top of this reactor 3 is provided with through hole (not shown).This electric mixing device 7 is located in the cavity of reactor 3, and is positioned on base 4.Dissolved oxygen probe 2 is wherein located in the cavity of reactor 3, and is positioned at the middle and upper part of reactor 3, and it is connected with data collector 1, for measuring the dissolved oxygen concentration of mud, and then calculates its respiratory rate.
The course of work of original position respiratory rate instrument of the present invention is as follows:
To transferring wire rope 6, the base 4 of reactor 3 declines due to gravity, and now reactor 3 times edges are opened wide from base 4, and internal liquid and external environment start to exchange.Mobile pole 5, is put into specified measurement point together with reactor 3, mentions wire rope 6, is closed in base 4 and reactor 3 times edges, and this process completes the sampling of this measurement point.After closed, open stirring apparatus 7 immediately, sampling liquid is stirred.Start the dissolved oxygen concentration recording sampling mixed liquor, continue 1 ~ 3 minute.Computing machine 1 calculates respiratory rate after recording whole mensuration process data.Close stirring apparatus 7, then wire rope 6 is transferred, mixed liquor in emptying reactor 3, for test is prepared next time.
Utilize original position respiratory rate instrument of the present invention to carry out measuring the respiratory rate of somewhere mud, curve is as shown in Figure 2 over time for it.
Adopt the standard method of hermetical testing in contrast, measure the respiratory rate of same place mud.Use sampling thief to obtain mixed liquor from measuring position, fill 250mL conical flask, in bottle, put into magnetic stir bar, insert dissolved oxygen probe at bottleneck and seal, make mixed liquor be full of bottle and and isolate from outer air.Entirety is placed on magnetic stirring apparatus, and adjustment magnetic agitation rotor speed, makes mixed liquor in bottle mix completely.Now gather the concentration data of dissolved oxygen probe, test result as shown in Figure 2.
As seen from Figure 2, method of the present invention and existing standard method have good consistance.Because within 0 ~ 20 second of test beginning, because dissolved oxygen instrument sonde response exists the problems such as delayed, dissolved oxygen DO rate of change presents quick decline.After 20 seconds, rate of change is stablized gradually, and dissolved oxygen instrument probe reaches steady-state response, and numerical value is now respiratory rate value, is 0.7mg/ (L.min) shown in figure.Therefore, 30 seconds later dissolved oxygen DO data can be got and calculate respiratory rate.
The respiratory rate of the active sludge of different depth in embodiment 2, mensuration biochemistry pool
The instantaneous respiratory rate at different depth place is measured at the aerobic section of certain sewage treatment plant AAO technique.
Three measurement points such as 0 meter, 1 meter, selected distance mixed liquor surface and 2 meters of, use the original position respiratory rate instrument in embodiment 1 to measure the instantaneous respiratory rate of above-mentioned measuring point, measure at every turn and completed at 60 seconds, after getting, 30 number of seconds are according to calculating respiratory rate.
Concrete testing procedure is according to the respiratory rate instrument use procedure in embodiment 1.Test result is respectively average 0.29mg/ (L.min), 0.14mg/ (L.min) and 0.39mg/ (L.min).
The respiratory rate difference that this embodiment reflects biochemistry pool different depth is obvious.
As shown in Figure 4 (a), the respiratory rate curve obtained as shown in Figure 4 (b) for the position view of the sampling spot of the present embodiment.
The respiratory rate of same degree of depth diverse location place active sludge in embodiment 3, mensuration biochemistry pool
, 1 meter, underwater is measured, the instantaneous respiratory rate of Different Plane position at the aerobic section of certain sewage treatment plant AAO technique.
Equally distributed 6 measuring points in selected aeration tank, use the original position respiratory rate instrument in embodiment 1 to measure the instantaneous respiratory rate of above-mentioned measuring point, and each measurement completed in 60 seconds, and after getting, 30 number of seconds are according to calculating respiratory rate.
Concrete testing procedure is according to the respiratory rate instrument use procedure in embodiment 1.Test result is respectively average 0.22mg/ (L.min), 0.23mg/ (L.min), 0.26mg/ (L.min), 0.31mg/ (L.min), 0.21mg/ (L.min) and 0.29mg/ (L.min).
This embodiment reflects the respiratory rate difference that biochemistry pool diverse location does not cause on an equal basis due to matrix mix variance, sludge concentration.
The position view of the sampling spot of the present embodiment is as shown in Fig. 5 (a), and the respiratory rate curve obtained is as shown in Fig. 5 (b).
Claims (7)
1. an original position respiratory rate instrument, is characterized in that: described respiratory rate instrument comprises a reactor, pole, stirring apparatus, dissolved oxygen probe and data collector;
Described reactor comprises can the base of folding; The base of described reactor is connected with described pole;
Described stirring stirring apparatus is located in described reactor;
Described dissolved oxygen probe is located in described reactor, and it is connected with described data collector.
2. original position respiratory rate instrument according to claim 1, is characterized in that: described pole is connected with described base by a wire rope.
3. original position respiratory rate instrument according to claim 1 and 2, is characterized in that: described stirring apparatus is located on described base.
4. the original position respiratory rate instrument according to any one of claim 1-3, is characterized in that: described dissolved oxygen probe is located at the middle and upper part of described reactor.
5. the original position respiratory rate instrument according to any one of claim 1-4, is characterized in that: the top of described reactor is provided with through hole.
6. utilize original position respiratory rate instrument according to any one of claim 1-5 to measure the method for the respiratory rate of active sludge, comprise the steps:
Described original position respiratory rate instrument is placed in the measurement point of active sludge, transfers described pole, make the body portion of described base and described reactor from, then the active sludge intermixture of tested point enters in described reactor;
Promote described pole, the main body of described base and described reactor is closed;
Start described stirring apparatus to stir, and simultaneously by the dissolved oxygen concentration of active sludge in reactor described in described data collector record; The dissolved oxygen concentration rate over time of calculated activity mud, the numerical value of the rate of change when described rate of change is stablized is the respiratory rate measuring active sludge.
7. method according to claim 6, is characterized in that: the time of described stirring is 1 ~ 3 minute.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107192800A (en) * | 2017-03-30 | 2017-09-22 | 南京大学盐城环保技术与工程研究院 | A kind of evaluation method of wastewater from chemical industry toxicity and biodegradability degree |
CN109266534A (en) * | 2018-08-28 | 2019-01-25 | 暨南大学 | A kind of bottle respirator for measuring biomembrane respiratory rate, measurement device and measuring method |
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CN102854221A (en) * | 2012-10-15 | 2013-01-02 | 中国科学院南京地理与湖泊研究所 | In-situ measurement device and method for sediment breathing and nitrogen endogenous release |
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Patent Citations (2)
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CN102645353A (en) * | 2012-05-09 | 2012-08-22 | 河海大学常州校区 | Rotary sealing bottom type superficial layer in-situ sediment sampler |
CN102854221A (en) * | 2012-10-15 | 2013-01-02 | 中国科学院南京地理与湖泊研究所 | In-situ measurement device and method for sediment breathing and nitrogen endogenous release |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107192800A (en) * | 2017-03-30 | 2017-09-22 | 南京大学盐城环保技术与工程研究院 | A kind of evaluation method of wastewater from chemical industry toxicity and biodegradability degree |
CN109266534A (en) * | 2018-08-28 | 2019-01-25 | 暨南大学 | A kind of bottle respirator for measuring biomembrane respiratory rate, measurement device and measuring method |
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