CN208917210U - A kind of cell culture apparatus - Google Patents
A kind of cell culture apparatus Download PDFInfo
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- CN208917210U CN208917210U CN201821602699.9U CN201821602699U CN208917210U CN 208917210 U CN208917210 U CN 208917210U CN 201821602699 U CN201821602699 U CN 201821602699U CN 208917210 U CN208917210 U CN 208917210U
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- biological respinse
- respinse bag
- bag
- cell culture
- discharge nozzle
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- 238000004113 cell culture Methods 0.000 title claims abstract description 40
- 238000001802 infusion Methods 0.000 claims abstract description 21
- 239000012530 fluid Substances 0.000 claims abstract description 17
- 238000011081 inoculation Methods 0.000 claims abstract description 17
- 239000000523 sample Substances 0.000 claims description 46
- 230000010412 perfusion Effects 0.000 claims description 39
- 238000003756 stirring Methods 0.000 claims description 28
- 239000002002 slurry Substances 0.000 claims description 12
- 239000000356 contaminant Substances 0.000 abstract description 3
- 238000011017 operating method Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 15
- 238000000034 method Methods 0.000 description 9
- 230000005484 gravity Effects 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 239000006143 cell culture medium Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 230000002572 peristaltic effect Effects 0.000 description 5
- 238000007599 discharging Methods 0.000 description 4
- 230000002427 irreversible effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000001394 metastastic effect Effects 0.000 description 4
- 206010061289 metastatic neoplasm Diseases 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000012549 training Methods 0.000 description 3
- 238000005299 abrasion Methods 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011169 microbiological contamination Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000002459 sustained effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a kind of cell culture apparatus.Specifically, the utility model provides a kind of cell culture apparatus, and described device includes fluid infusion portion, inoculation portion, culture portion；Fluid infusion portion includes the first biological respinse bag and the first container for being used to support the first biological respinse bag；Culture portion includes the second biological respinse bag of multiple and different volume sizes and the second container for being used to support each second biological respinse bag；Inoculation portion includes a vortex mixer, lays third biological respinse bag on vortex mixer；On the basis of horizontal plane, the height of the first biological respinse bag and third biological respinse bag is respectively higher than each second biological respinse bag, and the height of each second biological respinse bag is successively increased according to the sequence of volume from big to small.The utility model cell culture apparatus can reduce operating procedure, it is easy to operate, cut operating costs and can be avoided living contaminants.
Description
Technical field
The utility model relates to bio-pharmaceutical industries, more particularly to a kind of cell culture apparatus.
Background technique
During bio-pharmaceuticals, cell culture is often referred in cell fermentation, in existing industrial production mistake
Cheng Zhong is mounted on sustained height and puts by widened sequence step by step usually by the cell culture apparatus of different scales, using outer
Power acts on (such as peristaltic pump, diaphragm pump) metastatic cells culture solution and cell liquid, but this method often has many defects, main
Include:
1. needing to be transported through using external force equipment, influence to cause vulnerable to external force some irreversible and unpredictable
Damage.2. sustained height puts the cell culture apparatus of different scales before not eventually entering into production and closing, pipeline Yi Yuke
The place contact that can be polluted.3. it is complicated for operation, it needs first to make pipeline and pipeline sterilizes.Divide by pipeline both ends
It is not connected with two culture devices.Then, pipeline is placed in peristaltic pump to and is confirmed traffic direction and adjusts flow velocity.4. runing into
This height is transported through using external force equipment such as peristaltic pump, the pipe for needing use to match when pumping operating.Risk is reduced, it is long
Time easily causes abrasion to rupture using peristaltic pump in same position, causes microbiological contamination risk.5. risk is big, peristaltic pump is used for a long time
It easily causes abrasion to rupture in same position, causes microbiological contamination risk.
Therefore, this field need to develop one kind can reduce operating procedure, it is easy to operate, cut operating costs and can keep away
Exempt from living contaminants cell culture apparatus.
Utility model content
The purpose of this utility model is to provide one kind can reduce operating procedure, it is easy to operate, cut operating costs and
It can be avoided living contaminants cell culture apparatus.
The utility model in a first aspect, provide a kind of cell culture apparatus, the device includes fluid infusion portion, inoculation
Portion, culture portion；
The fluid infusion portion includes the first biological respinse bag 10 and the first container 11 for being used to support the first biological respinse bag,
The first container is equipped with the probe of primary heater 12, first 13, first stirring driver 14, the first biological respinse bag
Equipped with first port access aperture 15, the first stirring slurry 16, the first feed pipe 17 and multiple first discharge nozzles 18, each first discharging
Pipe is across the first container, and each first discharge nozzle is equipped with the first control valve 19；
The culture portion includes the second biological respinse bag 20 of multiple and different volume sizes and is used to support each second
The second container 21 of biological respinse bag, the second container are equipped with the probe of secondary heater 22, second 23, second stirring driver
24, the second biological respinse bag is equipped with second port access aperture 25, the second stirring slurry 26, the second feed pipe 27 and second and goes out
Expects pipe 28, second discharge nozzle pass through the second container, and second discharge nozzle is equipped with the second control valve 29；
The inoculation portion includes a vortex mixer 30, lays third biological respinse bag 31 on the vortex mixer, described
Vortex mixer be equipped with third heater 32 and third probe 33, the third biological respinse bag be equipped with third port access aperture
34, third feed pipe 35 and third discharge nozzle 36, the third discharge nozzle are equipped with third control valve 37；
On the basis of horizontal plane, the height of the first biological respinse bag and the third biological respinse bag is above institute
Each second biological respinse bag is stated, and the height of each second biological respinse bag successively rises according to the sequence of volume from big to small
It is high；
Each first discharge nozzle of the first biological respinse bag and the second feed pipe of each second biological respinse bag
It is connected respectively by the first perfusion tube 40, pressure gauge 41, filter 42 and flow measuring unit 43 is additionally provided on first perfusion tube；
Second biological respinse bag of the second discharge nozzle and adjacent lower height of the second biological respinse bag of high height
Second feed pipe passes sequentially through the second perfusion tube 50 and is connected；
Second charging of the third discharge nozzle and the highest second biological respinse bag of height of the third biological respinse bag
Pipe is connected by third perfusion tube 60.
In another preferred example, first probe, the second probe and third probe pass through the first port respectively
Access aperture, second port access aperture and third port access aperture.
In another preferred example, the first container, second container and it is respectively equipped with through-hole, the first container,
The feed pipe and discharge nozzle of second container pass through the through-hole respectively.
In another preferred example, the sealed membrane is rubber membrane.
In another preferred example, the number of the port access aperture is 1-6.
In another preferred example, the biological respinse bag is disposable biological reaction bag.
In another preferred example, the biological respinse bag is XDR disposable biological reaction bag.
In another preferred example, the container is bucket.
In another preferred example, first probe, the second probe and third probe include pH probe and/or temperature
Probe.
In another preferred example, the number of the probe is 1-6.
In another preferred example, the vortex mixer is wave vortex mixer.
Have in preference another, the wave vortex mixer is the control of U.S. Thermo Scientifical light sensation
Wave vortex mixer.
In another preferred example, the stirring slurry is agitator arm.
In another preferred example, the agitator arm is plastic stir impeller.
In another preferred example, first control valve, the first control valve and third control valve are pipe clamp.
In another preferred example, the number of the second biological respinse bag is 2-6.
In another preferred example, the pressure gauge that first perfusion tube is equipped with is located at what first perfusion tube was equipped with
Between the filter and the first biological respinse bag.
In another preferred example, first perfusion tube, the second perfusion tube and third perfusion tube set insulating layer respectively.
In another preferred example, the device further includes a control unit.
In another preferred example, the control unit can be realized the automatic operating of cell culture apparatus.
It should be understood that in the scope of the utility model, above-mentioned each technical characteristic of the utility model and below (such as implementation
Example) in specifically describe each technical characteristic between can be combined with each other, to form a new or preferred technical solution.It is limited to
Length, not repeated them here.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of cell culture assembling device in a preference of the utility model.
Wherein, each number represents in attached drawing:
10 be the first biological respinse bag, 11 be the first container, 12 be primary heater, 13 be the first probe, 14 be first
Stirring driver, 15 be first port access aperture, 16 be the first stirring slurry, 17 be the first feed pipe, 18 be the first discharge nozzle, 19
It is the second biological respinse bag for the first control valve, 20,21 be second container, 22 be secondary heater, 23 be the second probe, 24 is
Second stirring driver, 25 be second port access aperture, 26 be the second stirring slurry, 27 be the second feed pipe, 28 be the second discharging
Pipe, 29 for the second control valve, 30 be vortex mixer, 31 be third biological respinse bag, 32 be third heater, 33 be third probe,
34 it is third port access aperture, 35 be third feed pipe, 36 be third discharge nozzle, 37 be third control valve, 40 is the first infusion
Pipe, 41 be pressure gauge, 42 be filter, 43 be flow measuring unit, 50 be the second perfusion tube, 60 be third perfusion tube.
Specific embodiment
The present inventor develops a kind of cell culture apparatus, in the utility model institute by extensive and in-depth study
In the cell culture assembling device stated, on the basis of horizontal plane, the first biological respinse bag and the third biological respinse bag
Height be above each second biological respinse bag, and the height of each second biological respinse bag according to volume from big to small
Sequence successively increase, there are differences in height between biological respinse bag and biological respinse bag, pass through self gravity factor metastatic cells
Culture solution and cell liquid, avoid in transport process, are influenced to cause some irreversible unpredictable damages by external force,
And the cell cultivation equipments of different scales is put before not eventually entering into production and closing by different height, without any pipeline
It is contacted with the place that may cause pollution.On this basis, inventor completes the utility model.
Term
Unless otherwise defined, belonging to the meaning and the utility model of all technical and scientific terms used herein
The meaning that field those of ordinary skill is commonly understood by is identical.
As used herein, the terms "include", "comprise" are used interchangeably with " containing ", not only include open definition, also
Including semi-enclosed and closed definition.In other words, the term include " by ... constitute ", " substantially by ... structure
At ".
As used herein, the orientation or positional relationship of the instructions such as term " on ", " horizontal plane " is side based on the figure
Position or positional relationship be merely for convenience of description the utility model and create and simplify description, rather than indication or suggestion is signified
Device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as practical new to this
The restriction that type is created.
As used herein, term " first ", " second ", " third " etc. are used for description purposes only, and should not be understood as indicating
Or it implies relative importance or implicitly indicates the quantity of indicated technical characteristic.Define as a result, " first ", " second ",
Features such as " thirds " can explicitly or implicitly include one or more of the features.
As used herein, term " cell culture apparatus " and " device " are meant that two or more.
As used herein, the meaning of " plurality " is two or more for term.
As used herein, term " the first, second, and third biological respinse bag " and " the first biological respinse bag, the second biology
The meaning of reaction bag and third biological respinse bag " is identical, can be replaced mutually.
For ease of description, further describe the utility model below with reference to Fig. 1, in the utility model it should be understood that
It is that attached drawing does not limit the scope of the utility model.
Typically, the utility model provides a kind of cell culture apparatus, and the device includes fluid infusion portion, inoculation portion, training
The portion of supporting；
The fluid infusion portion includes the first biological respinse bag 10 and the first container 11 for being used to support the first biological respinse bag,
The first container is equipped with the probe of primary heater 12, first 13, first stirring driver 14, the first biological respinse bag
Equipped with first port access aperture 15, the first stirring slurry 16, the first feed pipe 17 and multiple first discharge nozzles 18, each first discharging
Pipe is across the first container, and each first discharge nozzle is equipped with the first control valve 19；
The culture portion includes the second biological respinse bag 20 of multiple and different volume sizes and is used to support each second
The second container 21 of biological respinse bag, the second container are equipped with the probe of secondary heater 22, second 23, second stirring driver
24, the second biological respinse bag is equipped with second port access aperture 25, the second stirring slurry 26, the second feed pipe 27 and second and goes out
Expects pipe 28, second discharge nozzle pass through the second container, and second discharge nozzle is equipped with the second control valve 29；
The inoculation portion includes a vortex mixer 30, lays third biological respinse bag 31 on the vortex mixer, described
Vortex mixer be equipped with third heater 32 and third probe 33, the third biological respinse bag be equipped with third port access aperture
34, third feed pipe 35 and third discharge nozzle 36, the third discharge nozzle are equipped with third control valve 37；
On the basis of horizontal plane, the height of the first biological respinse bag and the third biological respinse bag is above institute
Each second biological respinse bag is stated, and the height of each second biological respinse bag successively rises according to the sequence of volume from big to small
It is high；
Each first discharge nozzle of the first biological respinse bag and the second feed pipe of each second biological respinse bag
It is connected respectively by the first perfusion tube 40, pressure gauge 41, filter 42 and flow measuring unit 43 is additionally provided on first perfusion tube；
Second biological respinse bag of the second discharge nozzle and adjacent lower height of the second biological respinse bag of high height
Second feed pipe passes sequentially through the second perfusion tube 50 and is connected；
Second charging of the third discharge nozzle and the highest second biological respinse bag of height of the third biological respinse bag
Pipe is connected by third perfusion tube 60.
In cell culture apparatus described in the utility model, on the basis of horizontal plane, the first biological respinse bag
Each second biological respinse bag, and each second biological respinse bag are above with the height point of the third biological respinse bag
Height successively increased according to the sequence of volume from big to small, between biological respinse bag and biological respinse bag there are difference in height,
It by self gravity metastatic cells culture solution and cell liquid, avoids in transport process, is influenced to cause by external force
Irreversible unpredictable damage.
In cell culture apparatus described in the utility model, heater can be to the first, second, and third biological respinse
Bag is heated, and maintains the temperature of biological respinse bag within the required range.
In cell culture apparatus described in the utility model, the probe on the first container, second container and vortex mixer is (such as
Probe) the first, second, and third biological respinse bag can be inserted by port access aperture, it is mended for detecting in biological respinse bag
Liquid, the pH of inoculation liquid and culture solution, temperature etc..Preferably, the probe includes pH probe and/or temp probe.Another
In preference, the first port access aperture, second port access aperture and third port access aperture are equipped with sealed membrane.It is described
First probe, second probe and third probe can be inserted by sealed membrane in biological respinse bag, in a typical case, warp
Probe after sterilizing is inserted into biological respinse bag by sealed membrane (when such as clinically fluid infusion, by the puncture needle of transfusion needle
Head is inserted into the infusion bottle with rubber stopper), to be further ensured that the aseptic of biological respinse bag.
In device described in the utility model, first perfusion tube, the second perfusion tube and third perfusion tube are equipped with
Insulating layer can guarantee fluid infusion, inoculation liquid and culture solution be not in during tube runs temperature reduction, to avoid
Temperature reduces the adverse effect generated to cell growth.
In device described in the utility model, first perfusion tube is equipped with filter, flow measuring unit and pressure gauge,
Flow measuring unit can control the first biological respinse bag fluid infusion to the amount of each second biological respinse bag, and filter can be to the first biology
The fluid infusion of reaction bag enters each second biological respinse bag after carrying out further sterilization treatment, to be further ensured that fluid infusion to the
The aseptic of two biological respinse bags, pressure gauge can be detected on the first perfusion tube by the pressure of the liquid of the first perfusion tube of induction
Whether damaged equipped with filter, such as the pressure gauge that the first perfusion tube is equipped with is located at the filtering that the first perfusion tube is equipped with
Between device and the first biological respinse bag, as the filter clogs, pressure gauge shows that pressure increases, when filter damage (filter hole
When becoming larger), pressure reduction.
In a preference of the utility model, the vortex mixer is wave vortex mixer.Wave vortex mixer can
It is waved by wave and the inoculation liquid in third biological respinse bag is mixed.
In another preferred example, the heater that the first container and second container are equipped with is electric heating collet.
In device described in the utility model, the stirring driver that the first container and second container are equipped with can drive
The stirring of the stirring slurry of one biological reaction bag and the second biological respinse bag, thus to liquid (fluid infusion and training in biological respinse bag
Nutrient solution) it is stirred, to improve efficiency.Preferably, the stirring slurry is agitator arm.It is highly preferred that the stirring
Impeller is plastic stir impeller.
In another preference of the utility model, the device further includes a control unit.Control unit can be realized carefully
The automatic operating of born of the same parents' culture apparatus.
The major advantage of the utility model includes:
1, in cell culture apparatus described in the utility model, on the basis of horizontal plane, first biological respinse
The height of bag and the third biological respinse bag is above each second biological respinse bag, and each second biological respinse bag
Height successively increased according to the sequence of volume from big to small, the high and low fall between biological respinse bag and biological respinse bag, lead to
Self gravity factor metastatic cells culture solution and cell liquid are crossed, is avoided in transport process, is influenced to cause one by external force
A little irreversible unpredictable damages, and do not eventually entered by the cell cultivation equipment that different height puts different scales
Before production is closed, contacted without any pipeline with the place that may cause pollution.
2, in cell culture apparatus described in the utility model, the first perfusion tube is equipped with flow measuring unit can accurate quantification control
It makes the fluid infusion in the first biological respinse bag and reaches amount in each second biological respinse bag.
The present invention will be further illustrated below in conjunction with specific embodiments.It should be understood that these embodiments are merely to illustrate this
Utility model rather than limitation the scope of the utility model.In the following examples, the experimental methods for specific conditions are not specified, leads to
Often according to normal conditions, or according to the normal condition proposed by manufacturer.Unless otherwise stated, otherwise percentage and number be by weight
It calculates.
Embodiment 1
Embodiment 1 provides a kind of cell culture apparatus, and the device includes fluid infusion portion, inoculation portion, culture portion；
The fluid infusion portion includes the first biological respinse bag and the first container for being used to support the first biological respinse bag, described
The first container is equipped with primary heater, the first probe (containing pH probe and temp probe), first stirs driver, and described the
One biological reaction bag is equipped with first port access aperture, the first agitator arm, the first feed pipe and multiple first discharge nozzles, Ge Ge
One discharge nozzle passes through the first container, and each first discharge nozzle is equipped with pipe clamp；
The culture portion includes that the second biological respinse bag of multiple and different volume sizes is given birth to being used to support each second
The second container of object reaction bag, the second container be equipped with secondary heater, second probe (containing pH pop one's head in and temp probe),
Second stirring driver, the second biological respinse bag are equipped with second port access aperture, the second agitator arm, the second feed pipe
With the second discharge nozzle, second discharge nozzle passes through the second container, and second discharge nozzle is equipped with pipe clamp；
The inoculation portion includes a wave vortex mixer, and the wave vortex mixer is equipped with secondary heater, the
Two probes (pop one's head in containing pH and temp probe), lay third biological respinse bag on the wave vortex mixer, and described the
Three biological reaction bags are equipped with third port access aperture, third feed pipe and third discharge nozzle, and the third discharge nozzle is equipped with
Pipe clamp；
On the basis of horizontal plane, the height of the first biological respinse bag and the third biological respinse bag is above institute
Each second biological respinse bag is stated, and the height of each second biological respinse bag successively rises according to the sequence of volume from big to small
It is high；
Each first discharge nozzle of the first biological respinse bag and the second feed pipe of each second biological respinse bag
It is connected respectively by the first perfusion tube, is additionally provided with pressure gauge, filter and flow measuring unit on first perfusion tube；
Second biological respinse bag of the second discharge nozzle and adjacent lower height of the second biological respinse bag of high height
Second feed pipe passes sequentially through the second perfusion tube and is connected；
Second charging of the third discharge nozzle and the highest second biological respinse bag of height of the third biological respinse bag
Pipe is connected by third perfusion tube；
Insulating layer is respectively equipped on first perfusion tube, the second infusion pipeline and third infusion pipeline.
The cell culture mechanism of cell culture apparatus described in the present embodiment specifically includes that
1, cell culture medium is prepared in the first biological respinse bag, prepares connecing containing aim cell in third biological respinse bag
Kind liquid, wherein in process for preparation, the heater of the first container and wave vortex mixer can be heated for cell culture medium and inoculation liquid
Heat preservation, the pH value and temperature of pH probe and temp probe monitoring cell culture medium and inoculation liquid process for preparation, the first container are stirred
The effect of waving for mixing the stirring action and wave vortex mixer of impeller can be improved preparation.
2, the pipe clamp for opening the discharge nozzle of the first biological respinse bag passes through gravity, the training of the first biological respinse bag cell
It supports base to be quantitatively adding in the highest second biological respinse bag of height by the first perfusion tube, then opens third biological respinse bag
Discharge nozzle pipe clamp, by gravity, the inoculation liquid of the first biological respinse bag fully enter height it is highest second biology
Reaction bag, therefore, cell are cultivated in the highest second biological respinse bag of height first, the highest second biological respinse bag of height
After middle cell culture, the pipe clamp of the highest second biological respinse bag of height is opened, by gravity, height highest the
Cell culture fluid enters in time high second biological respinse bag in two biological respinse bags, then opens and time high second biological respinse bag
The discharging pipe clamp of first biological respinse bag of connection, by gravity, the cell culture medium of the first biological respinse bag quantitatively adds
Enter into secondary high second biological respinse bag, cell continues to cultivate in secondary high second biological respinse bag, then repeats the above process, secondary
The cell culture fluid of high second biological respinse bag enters time time high second biological respinse bag culture, until reaching the cell culture
It is required that the second biological respinse bag of different volume sizes is in cell cultivation process, the heater of second container can heating and thermal insulation,
The pH value and temperature of pH probe and temp probe monitoring cell culture medium and inoculation liquid process for preparation, in addition, each second biology
The height of reaction bag is successively increased according to the sequence of volume from big to small, and therefore, cell is successively terraced in the second biological respinse bag
Degree amplification culture, to preferably realize industrialized production.
It is incorporated herein by reference in all documents that the utility model refers to, just as each document quilt
It is individually recited as with reference to such.In addition, it should also be understood that, after having read the above-mentioned teaching content of the utility model, this field skill
Art personnel can make various changes or modifications the utility model, and such equivalent forms equally fall within the application appended claims
Book limited range.
Claims (9)
1. a kind of cell culture apparatus, which is characterized in that the device includes fluid infusion portion, inoculation portion, culture portion；
The fluid infusion portion includes the first biological respinse bag (10) and is used to support the first container (11) of the first biological respinse bag,
The first container is equipped with primary heater (12), the first probe (13), the first stirring driver (14), first biology
Reaction bag is equipped with first port access aperture (15), the first stirring slurry (16), the first feed pipe (17) and multiple first discharge nozzles
(18), each first discharge nozzle passes through the first container, and each first discharge nozzle is equipped with the first control valve (19)；
The culture portion includes the second biological respinse bag (20) of multiple and different volume sizes and to be used to support each second raw
The second container (21) of object reaction bag, the second container are equipped with secondary heater (22), the second probe (23), the second stirring drive
Dynamic device (24), the second biological respinse bag are equipped with second port access aperture (25), the second stirring slurry (26), the second feed pipe
(27) second container is passed through, is set on second discharge nozzle with the second discharge nozzle (28), second discharge nozzle
There are the second control valve (29)；
The inoculation portion includes a vortex mixer (30), lays third biological respinse bag (31) on the vortex mixer, described
Vortex mixer be equipped with third heater (32) and third probe (33), the third biological respinse bag equipped with third port access
Hole (34), third feed pipe (35) and third discharge nozzle (36), the third discharge nozzle are equipped with third control valve (37)；
On the basis of horizontal plane, the height of the first biological respinse bag and the third biological respinse bag is above described each
A second biological respinse bag, and the height of each second biological respinse bag is successively increased according to the sequence of volume from big to small；
Each first discharge nozzle of the first biological respinse bag and the second feed pipe of each second biological respinse bag are distinguished
It is connected by the first perfusion tube (40), pressure gauge (41), filter (42) and flow measuring unit is additionally provided on first perfusion tube
(43)；
The second of second biological respinse bag of the second discharge nozzle and adjacent lower height of the second biological respinse bag of high height
Feed pipe passes sequentially through the second perfusion tube (50) road and is connected；
The third discharge nozzle of the third biological respinse bag and the second feed pipe of the highest second biological respinse bag of height are logical
Third perfusion tube (60) are crossed to be connected.
2. cell culture apparatus as described in claim 1, which is characterized in that the first probe, the second probe and the third
Probe includes pH probe and/or temp probe.
3. cell culture apparatus as described in claim 1, which is characterized in that the vortex mixer is wave vortex mixer.
4. cell culture apparatus as described in claim 1, which is characterized in that the stirring slurry is agitator arm.
5. cell culture apparatus as described in claim 1, which is characterized in that first control valve, the first control valve and
Third control valve is pipe clamp.
6. cell culture apparatus as described in claim 1, which is characterized in that the number of the second biological respinse bag is 2-
6.
7. cell culture apparatus as described in claim 1, which is characterized in that the pressure gauge position that first perfusion tube is equipped with
Between the filter and the first biological respinse bag that first perfusion tube is equipped with.
8. cell culture apparatus as described in claim 1, which is characterized in that first perfusion tube, the second perfusion tube and
Third perfusion tube sets insulating layer respectively.
9. cell culture apparatus as described in claim 1, which is characterized in that the device further includes a control unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821602699.9U CN208917210U (en) | 2018-09-29 | 2018-09-29 | A kind of cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821602699.9U CN208917210U (en) | 2018-09-29 | 2018-09-29 | A kind of cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208917210U true CN208917210U (en) | 2019-05-31 |
Family
ID=66707818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821602699.9U Active CN208917210U (en) | 2018-09-29 | 2018-09-29 | A kind of cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208917210U (en) |
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2018
- 2018-09-29 CN CN201821602699.9U patent/CN208917210U/en active Active
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